CLSTN1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLSTN1
Entrez Gene ID	102407320
Full Name	calsyntenin 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025285859.1	XP_025141644.1	calsyntenin-1 isoform X2
XM_025285860.1	XP_025141645.1	calsyntenin-1 isoform X3
XM_006076796.1	XP_006076858.1	calsyntenin-1
XM_025285858.1	XP_025141643.1	calsyntenin-1 isoform X1
XM_025285861.1	XP_025141646.1	calsyntenin-1 isoform X4

Pathways from BioSystems
Gene Ontology

The following CLSTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLSTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu66270	XM_025285858.1 Latest version!	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu66271	XM_025285859.1 Latest version!	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu66272	XM_025285860.1 Latest version!	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu66273	XM_025285861.1 Latest version!	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu30264	XM_006076796.1 Latest version!	Bubalus bubalis calsyntenin 1 (CLSTN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLSTN1 ( XM_025285858.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141643.1 Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu66270
Clone ID Related Accession (Same CDS sequence)	XM_025285858.1
Accession Version	XM_025285858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	calsyntenin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGAGAGTT TTGAGGTGAC AGTCACCAAA 
GAAGGTGAGA TTTGTGGATT TAAGATTCAT GGACAGAATG TGCCCTTTGA CGCAGTGGTG 
GTGGATAAAT CCACTGGCGA GGGGGTCATT CGTTCCACAG AGAAGCTGGA CTGTGAGCTG 
CAGAAGGACT ACACGTTCAC CATCCAGGCC TATGACTGCG GGAAGGGGCC AGACGGGGCC 
AATGTGAAAA AATCTCACAA AGCAACTGTC CACATTCAGG TGAACGACGT CAACGAATAT 
GCGCCCGTGT TCAAGGAGAA GTCCTACAAA GCCACAGTGG TGGAGGGGAA GCAGTACGAC 
AGCATCTTGA GGGTGGAGGC GGTGGACGCA GACTGCTCCC CCCAGTTCAG CCAAATCTGC 
AACTATGAGA TCGTCACCCC GGATGTGCCA TTCACCGTTG ACAAAGATGG TTACATTAAA 
AACACGGAGA AGCTGAACTA TGGTAAAGAG CACCAGTACA AGCTGACTGT CACAGCCTAC 
GACTGCGGGA AGAAAAGAGC GGCAGAAGAC GTTTTGGTGA AGATCAGCAT CAAGCCCACC 
TGCACCCCTG GGTGGCAAGG ATGGAACAAC AGGGTGGAAT ACGAGCCGGG CACTGGTGCT 
CTGACACTCT TTCCGAATGT CCACCTGGAA ACGTGTGACG AGTCGGTGGC CTCGGCACAG 
GTGGTGGTGG AGCTGGAGAC CAGCCACATC GGGAAGGGTT GTGACCGTGA CACCTACTCC 
GAGAAGTCAC TTCACCGGCT CTGCGGTGCT GCGTCTGGCA CGGCTGAGCT GCTACCTTCC 
CCAGGAGGCT CCTGGAACTG GACTATTGGC CTTCCCACGG ACAACGGCCA TGACAGCGAC 
CAGGTCTTCG AGTTCAACGG CACTCAGGCA GTGAGGGTCC CAGACAGCAT CGTCTCTGTC 
AACCCCAAGG AGCCCTTTAC GATCTCTGTG TGGATGAGGC ATGGGCCATT TGGCAAGAAG 
AAGGAGACGA TTGTTTGCAG CTCAGACAAA ACAGAGATGA ACCGACACCA TTACTCACTC 
TATGTCCATG GCTGCCGGCT GGTTTTCCTC CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA 
TACAAACCTG CAGAGTTCCA CTGGAAGCTG AGTCAGGTCT GTGACGAGGA ATGGCATCAC 
TACGTCCTCA ACGTGGAAAT CCCCAGTGTG ACTCTCTACG TCGACGGTGT CCCTCATGAG 
CCCTTCTCTG TCACTGAAGA TTACCCACTT CATCCATCCA AGATCGAAAC CCAGCTCGTG 
GTTGGGGCTT GCTGGCAAGA GTATTCAGGA GTTGAAAGTG ACAATGAAAC TGAGCCTGTG 
CCTCTGGCCT CTGCAGGTGG TGACCTGCGT ATGACCCAGT TTTTCCGAGG TAATCTGGCT 
GGCCTGACCA TCCGTTCTGG GAAGCTCACA GATAAGAAGG TGATTGACTG TCTGTATACC 
TGCAAAGAGG GGCTAGACCT GCACACCCCC GAAGACAATG GCAGAGGTGT CAAGATCCAG 
GCCAACCCTA CTCAGTCGAT GTTGACCTTG GAGGGAGAGG ACGTGGGGGA GCTTGATAAG 
GCCATGCAGC ACATTTCCTA CCTTAATTCA CGACAGTTCC CCACCCCAGG GATCCGAAGA 
CTCAAAATCA CCAGCACAGT CAAGTGTTTT AACAAGGCCA CCTGCATCTC CATCCCCCCG 
GTGGATGGCT ACATAATGGT TTTGCAGCCA GAAGAGCCCA AGATCAGTCT GAGCGGCATC 
CACCATTTTG CTCGAGCGGC TTCCGAATTT GAAAGCTCGG AGGGGGTTTT CCTCTTCCCC 
GAGCTTCGGA TCATCAGCAC CATCACGAGA GAAGTGGAAC CCGAAGGGGA AGGGGATGAG 
GACCCAACAG TTCAAGAGTC ACTGGTGTCA GAGGAGATCG TGCACGACCT GGACACCTGT 
GAGGTCACAG TGGAGGGAGA AGAACTGAAT GGAGAGCAGG AGAGCCTGGA GGTGGACGCG 
GCCCGCCTGC AGCAGAAGGG CATCGAAATG AGCAGCTCTG ACATGGGCGT GGTCTTCACA 
GGTGTTGACA CCATGGCCAG CTATGAGGAG ATCTTACACC TACTGCGCTA TCGGAACTGG 
CACACCAGGT CCTTGCTTGA CCGCAAGTTC AAGCTTGTCT GCTCTGAGCT GAATGGTCGC 
TATGTCAGCA ACGAGTTCCA GGTGGAGGTG AATGTCATCC ACACAGCCAA CCCCATGGAG 
CACGCCAGCC ACATAGCTGC CCAGCCACAG TTCGTCCACC CCGAGCACCG CTCCTTCATT 
GACCTCTCGG GTCACAACCT GGCCAACCCC CACCCGTTTG CAGTCGTGCC CAGCACGGCC 
ACCGTCGTCA TCGTGGTGTG CGTCAGCTTC CTGGTTTTCA TGATCATCCT GGGGGTGTTC 
CGGATCCGGG CTGCACACCA GAGAACCATG CGGGACCAGG ACACCGGGAA AGAGAATGAG 
ATGGACTGGG ATGACTCTGC CCTGACCATC ACCGTGAACC CCATGGAGAC ATACGAGGAC 
CAGCATAGCA GTGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGAGCGAGGA TGGGGACGAG 
GAGGACGACA TCACCAGTGC TGAGTCGGAG AGCAGTGAGG AGGACGAGGG GGAGCACGGG 
GACCCGCAGA CCGCGAACCG GCAGCAGCAG CTGGAGTGGG ACGACTCCAC CCTCAGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141643.1
CDS	60..3002
Translation	MLRRPAPALAPAAWLLLAGLLCSGGVWASRVNKHKPWLEPTYHGLVTESDSTVLLDPPLIALDKDAPLRFAESFEVTVTKEGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSTEKLDCELQKDYTFTIQAYDCGKGPDGANVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVVEGKQYDSILRVEAVDADCSPQFSQICNYEIVTPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRAAEDVLVKISIKPTCTPGWQGWNNRVEYEPGTGALTLFPNVHLETCDESVASAQVVVELETSHIGKGCDRDTYSEKSLHRLCGAASGTAELLPSPGGSWNWTIGLPTDNGHDSDQVFEFNGTQAVRVPDSIVSVNPKEPFTISVWMRHGPFGKKKETIVCSSDKTEMNRHHYSLYVHGCRLVFLLRQDPSEEKKYKPAEFHWKLSQVCDEEWHHYVLNVEIPSVTLYVDGVPHEPFSVTEDYPLHPSKIETQLVVGACWQEYSGVESDNETEPVPLASAGGDLRMTQFFRGNLAGLTIRSGKLTDKKVIDCLYTCKEGLDLHTPEDNGRGVKIQANPTQSMLTLEGEDVGELDKAMQHISYLNSRQFPTPGIRRLKITSTVKCFNKATCISIPPVDGYIMVLQPEEPKISLSGIHHFARAASEFESSEGVFLFPELRIISTITREVEPEGEGDEDPTVQESLVSEEIVHDLDTCEVTVEGEELNGEQESLEVDAARLQQKGIEMSSSDMGVVFTGVDTMASYEEILHLLRYRNWHTRSLLDRKFKLVCSELNGRYVSNEFQVEVNVIHTANPMEHASHIAAQPQFVHPEHRSFIDLSGHNLANPHPFAVVPSTATVVIVVCVSFLVFMIILGVFRIRAAHQRTMRDQDTGKENEMDWDDSALTITVNPMETYEDQHSSEEEEEEEEEESEDGDEEDDITSAESESSEEDEGEHGDPQTANRQQQLEWDDSTLSY

Target ORF information:

RefSeq Version	XM_025285858.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285858.1

ORF Insert Sequence:

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGAGAGTT TTGAGGTGAC AGTCACCAAA 
GAAGGTGAGA TTTGTGGATT TAAGATTCAT GGACAGAATG TGCCCTTTGA CGCAGTGGTG 
GTGGATAAAT CCACTGGCGA GGGGGTCATT CGTTCCACAG AGAAGCTGGA CTGTGAGCTG 
CAGAAGGACT ACACGTTCAC CATCCAGGCC TATGACTGCG GGAAGGGGCC AGACGGGGCC 
AATGTGAAAA AATCTCACAA AGCAACTGTC CACATTCAGG TGAACGACGT CAACGAATAT 
GCGCCCGTGT TCAAGGAGAA GTCCTACAAA GCCACAGTGG TGGAGGGGAA GCAGTACGAC 
AGCATCTTGA GGGTGGAGGC GGTGGACGCA GACTGCTCCC CCCAGTTCAG CCAAATCTGC 
AACTATGAGA TCGTCACCCC GGATGTGCCA TTCACCGTTG ACAAAGATGG TTACATTAAA 
AACACGGAGA AGCTGAACTA TGGTAAAGAG CACCAGTACA AGCTGACTGT CACAGCCTAC 
GACTGCGGGA AGAAAAGAGC GGCAGAAGAC GTTTTGGTGA AGATCAGCAT CAAGCCCACC 
TGCACCCCTG GGTGGCAAGG ATGGAACAAC AGGGTGGAAT ACGAGCCGGG CACTGGTGCT 
CTGACACTCT TTCCGAATGT CCACCTGGAA ACGTGTGACG AGTCGGTGGC CTCGGCACAG 
GTGGTGGTGG AGCTGGAGAC CAGCCACATC GGGAAGGGTT GTGACCGTGA CACCTACTCC 
GAGAAGTCAC TTCACCGGCT CTGCGGTGCT GCGTCTGGCA CGGCTGAGCT GCTACCTTCC 
CCAGGAGGCT CCTGGAACTG GACTATTGGC CTTCCCACGG ACAACGGCCA TGACAGCGAC 
CAGGTCTTCG AGTTCAACGG CACTCAGGCA GTGAGGGTCC CAGACAGCAT CGTCTCTGTC 
AACCCCAAGG AGCCCTTTAC GATCTCTGTG TGGATGAGGC ATGGGCCATT TGGCAAGAAG 
AAGGAGACGA TTGTTTGCAG CTCAGACAAA ACAGAGATGA ACCGACACCA TTACTCACTC 
TATGTCCATG GCTGCCGGCT GGTTTTCCTC CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA 
TACAAACCTG CAGAGTTCCA CTGGAAGCTG AGTCAGGTCT GTGACGAGGA ATGGCATCAC 
TACGTCCTCA ACGTGGAAAT CCCCAGTGTG ACTCTCTACG TCGACGGTGT CCCTCATGAG 
CCCTTCTCTG TCACTGAAGA TTACCCACTT CATCCATCCA AGATCGAAAC CCAGCTCGTG 
GTTGGGGCTT GCTGGCAAGA GTATTCAGGA GTTGAAAGTG ACAATGAAAC TGAGCCTGTG 
CCTCTGGCCT CTGCAGGTGG TGACCTGCGT ATGACCCAGT TTTTCCGAGG TAATCTGGCT 
GGCCTGACCA TCCGTTCTGG GAAGCTCACA GATAAGAAGG TGATTGACTG TCTGTATACC 
TGCAAAGAGG GGCTAGACCT GCACACCCCC GAAGACAATG GCAGAGGTGT CAAGATCCAG 
GCCAACCCTA CTCAGTCGAT GTTGACCTTG GAGGGAGAGG ACGTGGGGGA GCTTGATAAG 
GCCATGCAGC ACATTTCCTA CCTTAATTCA CGACAGTTCC CCACCCCAGG GATCCGAAGA 
CTCAAAATCA CCAGCACAGT CAAGTGTTTT AACAAGGCCA CCTGCATCTC CATCCCCCCG 
GTGGATGGCT ACATAATGGT TTTGCAGCCA GAAGAGCCCA AGATCAGTCT GAGCGGCATC 
CACCATTTTG CTCGAGCGGC TTCCGAATTT GAAAGCTCGG AGGGGGTTTT CCTCTTCCCC 
GAGCTTCGGA TCATCAGCAC CATCACGAGA GAAGTGGAAC CCGAAGGGGA AGGGGATGAG 
GACCCAACAG TTCAAGAGTC ACTGGTGTCA GAGGAGATCG TGCACGACCT GGACACCTGT 
GAGGTCACAG TGGAGGGAGA AGAACTGAAT GGAGAGCAGG AGAGCCTGGA GGTGGACGCG 
GCCCGCCTGC AGCAGAAGGG CATCGAAATG AGCAGCTCTG ACATGGGCGT GGTCTTCACA 
GGTGTTGACA CCATGGCCAG CTATGAGGAG ATCTTACACC TACTGCGCTA TCGGAACTGG 
CACACCAGGT CCTTGCTTGA CCGCAAGTTC AAGCTTGTCT GCTCTGAGCT GAATGGTCGC 
TATGTCAGCA ACGAGTTCCA GGTGGAGGTG AATGTCATCC ACACAGCCAA CCCCATGGAG 
CACGCCAGCC ACATAGCTGC CCAGCCACAG TTCGTCCACC CCGAGCACCG CTCCTTCATT 
GACCTCTCGG GTCACAACCT GGCCAACCCC CACCCGTTTG CAGTCGTGCC CAGCACGGCC 
ACCGTCGTCA TCGTGGTGTG CGTCAGCTTC CTGGTTTTCA TGATCATCCT GGGGGTGTTC 
CGGATCCGGG CTGCACACCA GAGAACCATG CGGGACCAGG ACACCGGGAA AGAGAATGAG 
ATGGACTGGG ATGACTCTGC CCTGACCATC ACCGTGAACC CCATGGAGAC ATACGAGGAC 
CAGCATAGCA GTGAGGAGGA AGAGGAGGAG GAGGAAGAGG AGAGCGAGGA TGGGGACGAG 
GAGGACGACA TCACCAGTGC TGAGTCGGAG AGCAGTGAGG AGGACGAGGG GGAGCACGGG 
GACCCGCAGA CCGCGAACCG GCAGCAGCAG CTGGAGTGGG ACGACTCCAC CCTCAGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLSTN1 ( XM_025285859.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141644.1 Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu66271
Clone ID Related Accession (Same CDS sequence)	XM_025285859.1
Accession Version	XM_025285859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	calsyntenin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGGTGAGA TTTGTGGATT TAAGATTCAT 
GGACAGAATG TGCCCTTTGA CGCAGTGGTG GTGGATAAAT CCACTGGCGA GGGGGTCATT 
CGTTCCACAG AGAAGCTGGA CTGTGAGCTG CAGAAGGACT ACACGTTCAC CATCCAGGCC 
TATGACTGCG GGAAGGGGCC AGACGGGGCC AATGTGAAAA AATCTCACAA AGCAACTGTC 
CACATTCAGG TGAACGACGT CAACGAATAT GCGCCCGTGT TCAAGGAGAA GTCCTACAAA 
GCCACAGTGG TGGAGGGGAA GCAGTACGAC AGCATCTTGA GGGTGGAGGC GGTGGACGCA 
GACTGCTCCC CCCAGTTCAG CCAAATCTGC AACTATGAGA TCGTCACCCC GGATGTGCCA 
TTCACCGTTG ACAAAGATGG TTACATTAAA AACACGGAGA AGCTGAACTA TGGTAAAGAG 
CACCAGTACA AGCTGACTGT CACAGCCTAC GACTGCGGGA AGAAAAGAGC GGCAGAAGAC 
GTTTTGGTGA AGATCAGCAT CAAGCCCACC TGCACCCCTG GGTGGCAAGG ATGGAACAAC 
AGGGTGGAAT ACGAGCCGGG CACTGGTGCT CTGACACTCT TTCCGAATGT CCACCTGGAA 
ACGTGTGACG AGTCGGTGGC CTCGGCACAG GTGGTGGTGG AGCTGGAGAC CAGCCACATC 
GGGAAGGGTT GTGACCGTGA CACCTACTCC GAGAAGTCAC TTCACCGGCT CTGCGGTGCT 
GCGTCTGGCA CGGCTGAGCT GCTACCTTCC CCAGGAGGCT CCTGGAACTG GACTATTGGC 
CTTCCCACGG ACAACGGCCA TGACAGCGAC CAGGTCTTCG AGTTCAACGG CACTCAGGCA 
GTGAGGGTCC CAGACAGCAT CGTCTCTGTC AACCCCAAGG AGCCCTTTAC GATCTCTGTG 
TGGATGAGGC ATGGGCCATT TGGCAAGAAG AAGGAGACGA TTGTTTGCAG CTCAGACAAA 
ACAGAGATGA ACCGACACCA TTACTCACTC TATGTCCATG GCTGCCGGCT GGTTTTCCTC 
CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA TACAAACCTG CAGAGTTCCA CTGGAAGCTG 
AGTCAGGTCT GTGACGAGGA ATGGCATCAC TACGTCCTCA ACGTGGAAAT CCCCAGTGTG 
ACTCTCTACG TCGACGGTGT CCCTCATGAG CCCTTCTCTG TCACTGAAGA TTACCCACTT 
CATCCATCCA AGATCGAAAC CCAGCTCGTG GTTGGGGCTT GCTGGCAAGA GTATTCAGGA 
GTTGAAAGTG ACAATGAAAC TGAGCCTGTG CCTCTGGCCT CTGCAGGTGG TGACCTGCGT 
ATGACCCAGT TTTTCCGAGG TAATCTGGCT GGCCTGACCA TCCGTTCTGG GAAGCTCACA 
GATAAGAAGG TGATTGACTG TCTGTATACC TGCAAAGAGG GGCTAGACCT GCACACCCCC 
GAAGACAATG GCAGAGGTGT CAAGATCCAG GCCAACCCTA CTCAGTCGAT GTTGACCTTG 
GAGGGAGAGG ACGTGGGGGA GCTTGATAAG GCCATGCAGC ACATTTCCTA CCTTAATTCA 
CGACAGTTCC CCACCCCAGG GATCCGAAGA CTCAAAATCA CCAGCACAGT CAAGTGTTTT 
AACAAGGCCA CCTGCATCTC CATCCCCCCG GTGGATGGCT ACATAATGGT TTTGCAGCCA 
GAAGAGCCCA AGATCAGTCT GAGCGGCATC CACCATTTTG CTCGAGCGGC TTCCGAATTT 
GAAAGCTCGG AGGGGGTTTT CCTCTTCCCC GAGCTTCGGA TCATCAGCAC CATCACGAGA 
GAAGTGGAAC CCGAAGGGGA AGGGGATGAG GACCCAACAG TTCAAGAGTC ACTGGTGTCA 
GAGGAGATCG TGCACGACCT GGACACCTGT GAGGTCACAG TGGAGGGAGA AGAACTGAAT 
GGAGAGCAGG AGAGCCTGGA GGTGGACGCG GCCCGCCTGC AGCAGAAGGG CATCGAAATG 
AGCAGCTCTG ACATGGGCGT GGTCTTCACA GGTGTTGACA CCATGGCCAG CTATGAGGAG 
ATCTTACACC TACTGCGCTA TCGGAACTGG CACACCAGGT CCTTGCTTGA CCGCAAGTTC 
AAGCTTGTCT GCTCTGAGCT GAATGGTCGC TATGTCAGCA ACGAGTTCCA GGTGGAGGTG 
AATGTCATCC ACACAGCCAA CCCCATGGAG CACGCCAGCC ACATAGCTGC CCAGCCACAG 
TTCGTCCACC CCGAGCACCG CTCCTTCATT GACCTCTCGG GTCACAACCT GGCCAACCCC 
CACCCGTTTG CAGTCGTGCC CAGCACGGCC ACCGTCGTCA TCGTGGTGTG CGTCAGCTTC 
CTGGTTTTCA TGATCATCCT GGGGGTGTTC CGGATCCGGG CTGCACACCA GAGAACCATG 
CGGGACCAGG ACACCGGGAA AGAGAATGAG ATGGACTGGG ATGACTCTGC CCTGACCATC 
ACCGTGAACC CCATGGAGAC ATACGAGGAC CAGCATAGCA GTGAGGAGGA AGAGGAGGAG 
GAGGAAGAGG AGAGCGAGGA TGGGGACGAG GAGGACGACA TCACCAGTGC TGAGTCGGAG 
AGCAGTGAGG AGGACGAGGG GGAGCACGGG GACCCGCAGA CCGCGAACCG GCAGCAGCAG 
CTGGAGTGGG ACGACTCCAC CCTCAGCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141644.1
CDS	59..2971
Translation	MLRRPAPALAPAAWLLLAGLLCSGGVWASRVNKHKPWLEPTYHGLVTESDSTVLLDPPLIALDKDAPLRFAGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSTEKLDCELQKDYTFTIQAYDCGKGPDGANVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVVEGKQYDSILRVEAVDADCSPQFSQICNYEIVTPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRAAEDVLVKISIKPTCTPGWQGWNNRVEYEPGTGALTLFPNVHLETCDESVASAQVVVELETSHIGKGCDRDTYSEKSLHRLCGAASGTAELLPSPGGSWNWTIGLPTDNGHDSDQVFEFNGTQAVRVPDSIVSVNPKEPFTISVWMRHGPFGKKKETIVCSSDKTEMNRHHYSLYVHGCRLVFLLRQDPSEEKKYKPAEFHWKLSQVCDEEWHHYVLNVEIPSVTLYVDGVPHEPFSVTEDYPLHPSKIETQLVVGACWQEYSGVESDNETEPVPLASAGGDLRMTQFFRGNLAGLTIRSGKLTDKKVIDCLYTCKEGLDLHTPEDNGRGVKIQANPTQSMLTLEGEDVGELDKAMQHISYLNSRQFPTPGIRRLKITSTVKCFNKATCISIPPVDGYIMVLQPEEPKISLSGIHHFARAASEFESSEGVFLFPELRIISTITREVEPEGEGDEDPTVQESLVSEEIVHDLDTCEVTVEGEELNGEQESLEVDAARLQQKGIEMSSSDMGVVFTGVDTMASYEEILHLLRYRNWHTRSLLDRKFKLVCSELNGRYVSNEFQVEVNVIHTANPMEHASHIAAQPQFVHPEHRSFIDLSGHNLANPHPFAVVPSTATVVIVVCVSFLVFMIILGVFRIRAAHQRTMRDQDTGKENEMDWDDSALTITVNPMETYEDQHSSEEEEEEEEEESEDGDEEDDITSAESESSEEDEGEHGDPQTANRQQQLEWDDSTLSY

Target ORF information:

RefSeq Version	XM_025285859.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285859.1

ORF Insert Sequence:

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGGTGAGA TTTGTGGATT TAAGATTCAT 
GGACAGAATG TGCCCTTTGA CGCAGTGGTG GTGGATAAAT CCACTGGCGA GGGGGTCATT 
CGTTCCACAG AGAAGCTGGA CTGTGAGCTG CAGAAGGACT ACACGTTCAC CATCCAGGCC 
TATGACTGCG GGAAGGGGCC AGACGGGGCC AATGTGAAAA AATCTCACAA AGCAACTGTC 
CACATTCAGG TGAACGACGT CAACGAATAT GCGCCCGTGT TCAAGGAGAA GTCCTACAAA 
GCCACAGTGG TGGAGGGGAA GCAGTACGAC AGCATCTTGA GGGTGGAGGC GGTGGACGCA 
GACTGCTCCC CCCAGTTCAG CCAAATCTGC AACTATGAGA TCGTCACCCC GGATGTGCCA 
TTCACCGTTG ACAAAGATGG TTACATTAAA AACACGGAGA AGCTGAACTA TGGTAAAGAG 
CACCAGTACA AGCTGACTGT CACAGCCTAC GACTGCGGGA AGAAAAGAGC GGCAGAAGAC 
GTTTTGGTGA AGATCAGCAT CAAGCCCACC TGCACCCCTG GGTGGCAAGG ATGGAACAAC 
AGGGTGGAAT ACGAGCCGGG CACTGGTGCT CTGACACTCT TTCCGAATGT CCACCTGGAA 
ACGTGTGACG AGTCGGTGGC CTCGGCACAG GTGGTGGTGG AGCTGGAGAC CAGCCACATC 
GGGAAGGGTT GTGACCGTGA CACCTACTCC GAGAAGTCAC TTCACCGGCT CTGCGGTGCT 
GCGTCTGGCA CGGCTGAGCT GCTACCTTCC CCAGGAGGCT CCTGGAACTG GACTATTGGC 
CTTCCCACGG ACAACGGCCA TGACAGCGAC CAGGTCTTCG AGTTCAACGG CACTCAGGCA 
GTGAGGGTCC CAGACAGCAT CGTCTCTGTC AACCCCAAGG AGCCCTTTAC GATCTCTGTG 
TGGATGAGGC ATGGGCCATT TGGCAAGAAG AAGGAGACGA TTGTTTGCAG CTCAGACAAA 
ACAGAGATGA ACCGACACCA TTACTCACTC TATGTCCATG GCTGCCGGCT GGTTTTCCTC 
CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA TACAAACCTG CAGAGTTCCA CTGGAAGCTG 
AGTCAGGTCT GTGACGAGGA ATGGCATCAC TACGTCCTCA ACGTGGAAAT CCCCAGTGTG 
ACTCTCTACG TCGACGGTGT CCCTCATGAG CCCTTCTCTG TCACTGAAGA TTACCCACTT 
CATCCATCCA AGATCGAAAC CCAGCTCGTG GTTGGGGCTT GCTGGCAAGA GTATTCAGGA 
GTTGAAAGTG ACAATGAAAC TGAGCCTGTG CCTCTGGCCT CTGCAGGTGG TGACCTGCGT 
ATGACCCAGT TTTTCCGAGG TAATCTGGCT GGCCTGACCA TCCGTTCTGG GAAGCTCACA 
GATAAGAAGG TGATTGACTG TCTGTATACC TGCAAAGAGG GGCTAGACCT GCACACCCCC 
GAAGACAATG GCAGAGGTGT CAAGATCCAG GCCAACCCTA CTCAGTCGAT GTTGACCTTG 
GAGGGAGAGG ACGTGGGGGA GCTTGATAAG GCCATGCAGC ACATTTCCTA CCTTAATTCA 
CGACAGTTCC CCACCCCAGG GATCCGAAGA CTCAAAATCA CCAGCACAGT CAAGTGTTTT 
AACAAGGCCA CCTGCATCTC CATCCCCCCG GTGGATGGCT ACATAATGGT TTTGCAGCCA 
GAAGAGCCCA AGATCAGTCT GAGCGGCATC CACCATTTTG CTCGAGCGGC TTCCGAATTT 
GAAAGCTCGG AGGGGGTTTT CCTCTTCCCC GAGCTTCGGA TCATCAGCAC CATCACGAGA 
GAAGTGGAAC CCGAAGGGGA AGGGGATGAG GACCCAACAG TTCAAGAGTC ACTGGTGTCA 
GAGGAGATCG TGCACGACCT GGACACCTGT GAGGTCACAG TGGAGGGAGA AGAACTGAAT 
GGAGAGCAGG AGAGCCTGGA GGTGGACGCG GCCCGCCTGC AGCAGAAGGG CATCGAAATG 
AGCAGCTCTG ACATGGGCGT GGTCTTCACA GGTGTTGACA CCATGGCCAG CTATGAGGAG 
ATCTTACACC TACTGCGCTA TCGGAACTGG CACACCAGGT CCTTGCTTGA CCGCAAGTTC 
AAGCTTGTCT GCTCTGAGCT GAATGGTCGC TATGTCAGCA ACGAGTTCCA GGTGGAGGTG 
AATGTCATCC ACACAGCCAA CCCCATGGAG CACGCCAGCC ACATAGCTGC CCAGCCACAG 
TTCGTCCACC CCGAGCACCG CTCCTTCATT GACCTCTCGG GTCACAACCT GGCCAACCCC 
CACCCGTTTG CAGTCGTGCC CAGCACGGCC ACCGTCGTCA TCGTGGTGTG CGTCAGCTTC 
CTGGTTTTCA TGATCATCCT GGGGGTGTTC CGGATCCGGG CTGCACACCA GAGAACCATG 
CGGGACCAGG ACACCGGGAA AGAGAATGAG ATGGACTGGG ATGACTCTGC CCTGACCATC 
ACCGTGAACC CCATGGAGAC ATACGAGGAC CAGCATAGCA GTGAGGAGGA AGAGGAGGAG 
GAGGAAGAGG AGAGCGAGGA TGGGGACGAG GAGGACGACA TCACCAGTGC TGAGTCGGAG 
AGCAGTGAGG AGGACGAGGG GGAGCACGGG GACCCGCAGA CCGCGAACCG GCAGCAGCAG 
CTGGAGTGGG ACGACTCCAC CCTCAGCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLSTN1 ( XM_025285860.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141645.1 Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu66272
Clone ID Related Accession (Same CDS sequence)	XM_025285860.1
Accession Version	XM_025285860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	calsyntenin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGAGAGTT TTGAGGTGAC AGTCACCAAA 
GAAGGTGAGA TTTGTGGATT TAAGATTCAT GGACAGAATG TGCCCTTTGA CGCAGTGGTG 
GTGGATAAAT CCACTGGCGA GGGGGTCATT CGTTCCACAG AGAAGCTGGA CTGTGAGCTG 
CAGAAGGACT ACACGTTCAC CATCCAGGCC TATGACTGCG GGAAGGGGCC AGACGGGGCC 
AATGTGAAAA AATCTCACAA AGCAACTGTC CACATTCAGG TGAACGACGT CAACGAATAT 
GCGCCCGTGT TCAAGGAGAA GTCCTACAAA GCCACAGTGG TGGAGGGGAA GCAGTACGAC 
AGCATCTTGA GGGTGGAGGC GGTGGACGCA GACTGCTCCC CCCAGTTCAG CCAAATCTGC 
AACTATGAGA TCGTCACCCC GGATGTGCCA TTCACCGTTG ACAAAGATGG TTACATTAAA 
AACACGGAGA AGCTGAACTA TGGTAAAGAG CACCAGTACA AGCTGACTGT CACAGCCTAC 
GACTGCGGGA AGAAAAGAGC GGCAGAAGAC GTTTTGGTGA AGATCAGCAT CAAGCCCACC 
TGCACCCCTG GGTGGCAAGG ATGGAACAAC AGGGTGGAAT ACGAGCCGGG CACTGGTGCT 
CTGACACTCT TTCCGAATGT CCACCTGGAA ACGTGTGACG AGTCGGTGGC CTCGGCACAG 
GTGGTGGTGG AGCTGGAGAC CAGCCACATC GGGAAGGGTT GTGACCGTGA CACCTACTCC 
GAGAAGTCAC TTCACCGGCT CTGCGGTGCT GCGTCTGGCA CGGCTGAGCT GCTACCTTCC 
CCAGGAGGCT CCTGGAACTG GACTATTGGC CTTCCCACGG ACAACGGCCA TGACAGCGAC 
CAGGTCTTCG AGTTCAACGG CACTCAGGCA GTGAGGGTCC CAGACAGCAT CGTCTCTGTC 
AACCCCAAGG AGCCCTTTAC GATCTCTGTG TGGATGAGGC ATGGGCCATT TGGCAAGAAG 
AAGGAGACGA TTGTTTGCAG CTCAGACAAA ACAGAGATGA ACCGACACCA TTACTCACTC 
TATGTCCATG GCTGCCGGCT GGTTTTCCTC CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA 
TACAAACCTG CAGAGTTCCA CTGGAAGCTG AGTCAGGTCT GTGACGAGGA ATGGCATCAC 
TACGTCCTCA ACGTGGAAAT CCCCAGTGTG ACTCTCTACG TCGACGGTGT CCCTCATGAG 
CCCTTCTCTG TCACTGAAGA TTACCCACTT CATCCATCCA AGATCGAAAC CCAGCTCGTG 
GTTGGGGCTT GCTGGCAAGG TGGTGACCTG CGTATGACCC AGTTTTTCCG AGGTAATCTG 
GCTGGCCTGA CCATCCGTTC TGGGAAGCTC ACAGATAAGA AGGTGATTGA CTGTCTGTAT 
ACCTGCAAAG AGGGGCTAGA CCTGCACACC CCCGAAGACA ATGGCAGAGG TGTCAAGATC 
CAGGCCAACC CTACTCAGTC GATGTTGACC TTGGAGGGAG AGGACGTGGG GGAGCTTGAT 
AAGGCCATGC AGCACATTTC CTACCTTAAT TCACGACAGT TCCCCACCCC AGGGATCCGA 
AGACTCAAAA TCACCAGCAC AGTCAAGTGT TTTAACAAGG CCACCTGCAT CTCCATCCCC 
CCGGTGGATG GCTACATAAT GGTTTTGCAG CCAGAAGAGC CCAAGATCAG TCTGAGCGGC 
ATCCACCATT TTGCTCGAGC GGCTTCCGAA TTTGAAAGCT CGGAGGGGGT TTTCCTCTTC 
CCCGAGCTTC GGATCATCAG CACCATCACG AGAGAAGTGG AACCCGAAGG GGAAGGGGAT 
GAGGACCCAA CAGTTCAAGA GTCACTGGTG TCAGAGGAGA TCGTGCACGA CCTGGACACC 
TGTGAGGTCA CAGTGGAGGG AGAAGAACTG AATGGAGAGC AGGAGAGCCT GGAGGTGGAC 
GCGGCCCGCC TGCAGCAGAA GGGCATCGAA ATGAGCAGCT CTGACATGGG CGTGGTCTTC 
ACAGGTGTTG ACACCATGGC CAGCTATGAG GAGATCTTAC ACCTACTGCG CTATCGGAAC 
TGGCACACCA GGTCCTTGCT TGACCGCAAG TTCAAGCTTG TCTGCTCTGA GCTGAATGGT 
CGCTATGTCA GCAACGAGTT CCAGGTGGAG GTGAATGTCA TCCACACAGC CAACCCCATG 
GAGCACGCCA GCCACATAGC TGCCCAGCCA CAGTTCGTCC ACCCCGAGCA CCGCTCCTTC 
ATTGACCTCT CGGGTCACAA CCTGGCCAAC CCCCACCCGT TTGCAGTCGT GCCCAGCACG 
GCCACCGTCG TCATCGTGGT GTGCGTCAGC TTCCTGGTTT TCATGATCAT CCTGGGGGTG 
TTCCGGATCC GGGCTGCACA CCAGAGAACC ATGCGGGACC AGGACACCGG GAAAGAGAAT 
GAGATGGACT GGGATGACTC TGCCCTGACC ATCACCGTGA ACCCCATGGA GACATACGAG 
GACCAGCATA GCAGTGAGGA GGAAGAGGAG GAGGAGGAAG AGGAGAGCGA GGATGGGGAC 
GAGGAGGACG ACATCACCAG TGCTGAGTCG GAGAGCAGTG AGGAGGACGA GGGGGAGCAC 
GGGGACCCGC AGACCGCGAA CCGGCAGCAG CAGCTGGAGT GGGACGACTC CACCCTCAGC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141645.1
CDS	59..2944
Translation	MLRRPAPALAPAAWLLLAGLLCSGGVWASRVNKHKPWLEPTYHGLVTESDSTVLLDPPLIALDKDAPLRFAESFEVTVTKEGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSTEKLDCELQKDYTFTIQAYDCGKGPDGANVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVVEGKQYDSILRVEAVDADCSPQFSQICNYEIVTPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRAAEDVLVKISIKPTCTPGWQGWNNRVEYEPGTGALTLFPNVHLETCDESVASAQVVVELETSHIGKGCDRDTYSEKSLHRLCGAASGTAELLPSPGGSWNWTIGLPTDNGHDSDQVFEFNGTQAVRVPDSIVSVNPKEPFTISVWMRHGPFGKKKETIVCSSDKTEMNRHHYSLYVHGCRLVFLLRQDPSEEKKYKPAEFHWKLSQVCDEEWHHYVLNVEIPSVTLYVDGVPHEPFSVTEDYPLHPSKIETQLVVGACWQGGDLRMTQFFRGNLAGLTIRSGKLTDKKVIDCLYTCKEGLDLHTPEDNGRGVKIQANPTQSMLTLEGEDVGELDKAMQHISYLNSRQFPTPGIRRLKITSTVKCFNKATCISIPPVDGYIMVLQPEEPKISLSGIHHFARAASEFESSEGVFLFPELRIISTITREVEPEGEGDEDPTVQESLVSEEIVHDLDTCEVTVEGEELNGEQESLEVDAARLQQKGIEMSSSDMGVVFTGVDTMASYEEILHLLRYRNWHTRSLLDRKFKLVCSELNGRYVSNEFQVEVNVIHTANPMEHASHIAAQPQFVHPEHRSFIDLSGHNLANPHPFAVVPSTATVVIVVCVSFLVFMIILGVFRIRAAHQRTMRDQDTGKENEMDWDDSALTITVNPMETYEDQHSSEEEEEEEEEESEDGDEEDDITSAESESSEEDEGEHGDPQTANRQQQLEWDDSTLSY

Target ORF information:

RefSeq Version	XM_025285860.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285860.1

ORF Insert Sequence:

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGAGAGTT TTGAGGTGAC AGTCACCAAA 
GAAGGTGAGA TTTGTGGATT TAAGATTCAT GGACAGAATG TGCCCTTTGA CGCAGTGGTG 
GTGGATAAAT CCACTGGCGA GGGGGTCATT CGTTCCACAG AGAAGCTGGA CTGTGAGCTG 
CAGAAGGACT ACACGTTCAC CATCCAGGCC TATGACTGCG GGAAGGGGCC AGACGGGGCC 
AATGTGAAAA AATCTCACAA AGCAACTGTC CACATTCAGG TGAACGACGT CAACGAATAT 
GCGCCCGTGT TCAAGGAGAA GTCCTACAAA GCCACAGTGG TGGAGGGGAA GCAGTACGAC 
AGCATCTTGA GGGTGGAGGC GGTGGACGCA GACTGCTCCC CCCAGTTCAG CCAAATCTGC 
AACTATGAGA TCGTCACCCC GGATGTGCCA TTCACCGTTG ACAAAGATGG TTACATTAAA 
AACACGGAGA AGCTGAACTA TGGTAAAGAG CACCAGTACA AGCTGACTGT CACAGCCTAC 
GACTGCGGGA AGAAAAGAGC GGCAGAAGAC GTTTTGGTGA AGATCAGCAT CAAGCCCACC 
TGCACCCCTG GGTGGCAAGG ATGGAACAAC AGGGTGGAAT ACGAGCCGGG CACTGGTGCT 
CTGACACTCT TTCCGAATGT CCACCTGGAA ACGTGTGACG AGTCGGTGGC CTCGGCACAG 
GTGGTGGTGG AGCTGGAGAC CAGCCACATC GGGAAGGGTT GTGACCGTGA CACCTACTCC 
GAGAAGTCAC TTCACCGGCT CTGCGGTGCT GCGTCTGGCA CGGCTGAGCT GCTACCTTCC 
CCAGGAGGCT CCTGGAACTG GACTATTGGC CTTCCCACGG ACAACGGCCA TGACAGCGAC 
CAGGTCTTCG AGTTCAACGG CACTCAGGCA GTGAGGGTCC CAGACAGCAT CGTCTCTGTC 
AACCCCAAGG AGCCCTTTAC GATCTCTGTG TGGATGAGGC ATGGGCCATT TGGCAAGAAG 
AAGGAGACGA TTGTTTGCAG CTCAGACAAA ACAGAGATGA ACCGACACCA TTACTCACTC 
TATGTCCATG GCTGCCGGCT GGTTTTCCTC CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA 
TACAAACCTG CAGAGTTCCA CTGGAAGCTG AGTCAGGTCT GTGACGAGGA ATGGCATCAC 
TACGTCCTCA ACGTGGAAAT CCCCAGTGTG ACTCTCTACG TCGACGGTGT CCCTCATGAG 
CCCTTCTCTG TCACTGAAGA TTACCCACTT CATCCATCCA AGATCGAAAC CCAGCTCGTG 
GTTGGGGCTT GCTGGCAAGG TGGTGACCTG CGTATGACCC AGTTTTTCCG AGGTAATCTG 
GCTGGCCTGA CCATCCGTTC TGGGAAGCTC ACAGATAAGA AGGTGATTGA CTGTCTGTAT 
ACCTGCAAAG AGGGGCTAGA CCTGCACACC CCCGAAGACA ATGGCAGAGG TGTCAAGATC 
CAGGCCAACC CTACTCAGTC GATGTTGACC TTGGAGGGAG AGGACGTGGG GGAGCTTGAT 
AAGGCCATGC AGCACATTTC CTACCTTAAT TCACGACAGT TCCCCACCCC AGGGATCCGA 
AGACTCAAAA TCACCAGCAC AGTCAAGTGT TTTAACAAGG CCACCTGCAT CTCCATCCCC 
CCGGTGGATG GCTACATAAT GGTTTTGCAG CCAGAAGAGC CCAAGATCAG TCTGAGCGGC 
ATCCACCATT TTGCTCGAGC GGCTTCCGAA TTTGAAAGCT CGGAGGGGGT TTTCCTCTTC 
CCCGAGCTTC GGATCATCAG CACCATCACG AGAGAAGTGG AACCCGAAGG GGAAGGGGAT 
GAGGACCCAA CAGTTCAAGA GTCACTGGTG TCAGAGGAGA TCGTGCACGA CCTGGACACC 
TGTGAGGTCA CAGTGGAGGG AGAAGAACTG AATGGAGAGC AGGAGAGCCT GGAGGTGGAC 
GCGGCCCGCC TGCAGCAGAA GGGCATCGAA ATGAGCAGCT CTGACATGGG CGTGGTCTTC 
ACAGGTGTTG ACACCATGGC CAGCTATGAG GAGATCTTAC ACCTACTGCG CTATCGGAAC 
TGGCACACCA GGTCCTTGCT TGACCGCAAG TTCAAGCTTG TCTGCTCTGA GCTGAATGGT 
CGCTATGTCA GCAACGAGTT CCAGGTGGAG GTGAATGTCA TCCACACAGC CAACCCCATG 
GAGCACGCCA GCCACATAGC TGCCCAGCCA CAGTTCGTCC ACCCCGAGCA CCGCTCCTTC 
ATTGACCTCT CGGGTCACAA CCTGGCCAAC CCCCACCCGT TTGCAGTCGT GCCCAGCACG 
GCCACCGTCG TCATCGTGGT GTGCGTCAGC TTCCTGGTTT TCATGATCAT CCTGGGGGTG 
TTCCGGATCC GGGCTGCACA CCAGAGAACC ATGCGGGACC AGGACACCGG GAAAGAGAAT 
GAGATGGACT GGGATGACTC TGCCCTGACC ATCACCGTGA ACCCCATGGA GACATACGAG 
GACCAGCATA GCAGTGAGGA GGAAGAGGAG GAGGAGGAAG AGGAGAGCGA GGATGGGGAC 
GAGGAGGACG ACATCACCAG TGCTGAGTCG GAGAGCAGTG AGGAGGACGA GGGGGAGCAC 
GGGGACCCGC AGACCGCGAA CCGGCAGCAG CAGCTGGAGT GGGACGACTC CACCCTCAGC 
TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLSTN1 ( XM_025285861.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141646.1 Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu66273
Clone ID Related Accession (Same CDS sequence)	XM_025285861.1
Accession Version	XM_025285861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	calsyntenin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGGTGAGA TTTGTGGATT TAAGATTCAT 
GGACAGAATG TGCCCTTTGA CGCAGTGGTG GTGGATAAAT CCACTGGCGA GGGGGTCATT 
CGTTCCACAG AGAAGCTGGA CTGTGAGCTG CAGAAGGACT ACACGTTCAC CATCCAGGCC 
TATGACTGCG GGAAGGGGCC AGACGGGGCC AATGTGAAAA AATCTCACAA AGCAACTGTC 
CACATTCAGG TGAACGACGT CAACGAATAT GCGCCCGTGT TCAAGGAGAA GTCCTACAAA 
GCCACAGTGG TGGAGGGGAA GCAGTACGAC AGCATCTTGA GGGTGGAGGC GGTGGACGCA 
GACTGCTCCC CCCAGTTCAG CCAAATCTGC AACTATGAGA TCGTCACCCC GGATGTGCCA 
TTCACCGTTG ACAAAGATGG TTACATTAAA AACACGGAGA AGCTGAACTA TGGTAAAGAG 
CACCAGTACA AGCTGACTGT CACAGCCTAC GACTGCGGGA AGAAAAGAGC GGCAGAAGAC 
GTTTTGGTGA AGATCAGCAT CAAGCCCACC TGCACCCCTG GGTGGCAAGG ATGGAACAAC 
AGGGTGGAAT ACGAGCCGGG CACTGGTGCT CTGACACTCT TTCCGAATGT CCACCTGGAA 
ACGTGTGACG AGTCGGTGGC CTCGGCACAG GTGGTGGTGG AGCTGGAGAC CAGCCACATC 
GGGAAGGGTT GTGACCGTGA CACCTACTCC GAGAAGTCAC TTCACCGGCT CTGCGGTGCT 
GCGTCTGGCA CGGCTGAGCT GCTACCTTCC CCAGGAGGCT CCTGGAACTG GACTATTGGC 
CTTCCCACGG ACAACGGCCA TGACAGCGAC CAGGTCTTCG AGTTCAACGG CACTCAGGCA 
GTGAGGGTCC CAGACAGCAT CGTCTCTGTC AACCCCAAGG AGCCCTTTAC GATCTCTGTG 
TGGATGAGGC ATGGGCCATT TGGCAAGAAG AAGGAGACGA TTGTTTGCAG CTCAGACAAA 
ACAGAGATGA ACCGACACCA TTACTCACTC TATGTCCATG GCTGCCGGCT GGTTTTCCTC 
CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA TACAAACCTG CAGAGTTCCA CTGGAAGCTG 
AGTCAGGTCT GTGACGAGGA ATGGCATCAC TACGTCCTCA ACGTGGAAAT CCCCAGTGTG 
ACTCTCTACG TCGACGGTGT CCCTCATGAG CCCTTCTCTG TCACTGAAGA TTACCCACTT 
CATCCATCCA AGATCGAAAC CCAGCTCGTG GTTGGGGCTT GCTGGCAAGG TGGTGACCTG 
CGTATGACCC AGTTTTTCCG AGGTAATCTG GCTGGCCTGA CCATCCGTTC TGGGAAGCTC 
ACAGATAAGA AGGTGATTGA CTGTCTGTAT ACCTGCAAAG AGGGGCTAGA CCTGCACACC 
CCCGAAGACA ATGGCAGAGG TGTCAAGATC CAGGCCAACC CTACTCAGTC GATGTTGACC 
TTGGAGGGAG AGGACGTGGG GGAGCTTGAT AAGGCCATGC AGCACATTTC CTACCTTAAT 
TCACGACAGT TCCCCACCCC AGGGATCCGA AGACTCAAAA TCACCAGCAC AGTCAAGTGT 
TTTAACAAGG CCACCTGCAT CTCCATCCCC CCGGTGGATG GCTACATAAT GGTTTTGCAG 
CCAGAAGAGC CCAAGATCAG TCTGAGCGGC ATCCACCATT TTGCTCGAGC GGCTTCCGAA 
TTTGAAAGCT CGGAGGGGGT TTTCCTCTTC CCCGAGCTTC GGATCATCAG CACCATCACG 
AGAGAAGTGG AACCCGAAGG GGAAGGGGAT GAGGACCCAA CAGTTCAAGA GTCACTGGTG 
TCAGAGGAGA TCGTGCACGA CCTGGACACC TGTGAGGTCA CAGTGGAGGG AGAAGAACTG 
AATGGAGAGC AGGAGAGCCT GGAGGTGGAC GCGGCCCGCC TGCAGCAGAA GGGCATCGAA 
ATGAGCAGCT CTGACATGGG CGTGGTCTTC ACAGGTGTTG ACACCATGGC CAGCTATGAG 
GAGATCTTAC ACCTACTGCG CTATCGGAAC TGGCACACCA GGTCCTTGCT TGACCGCAAG 
TTCAAGCTTG TCTGCTCTGA GCTGAATGGT CGCTATGTCA GCAACGAGTT CCAGGTGGAG 
GTGAATGTCA TCCACACAGC CAACCCCATG GAGCACGCCA GCCACATAGC TGCCCAGCCA 
CAGTTCGTCC ACCCCGAGCA CCGCTCCTTC ATTGACCTCT CGGGTCACAA CCTGGCCAAC 
CCCCACCCGT TTGCAGCCGT CCTGTCTCCT CTTGCCCCAG TCGTGCCCAG CACGGCCACC 
GTCGTCATCG TGGTGTGCGT CAGCTTCCTG GTTTTCATGA TCATCCTGGG GGTGTTCCGG 
ATCCGGGCTG CACACCAGAG AACCATGCGG GACCAGGACA CCGGGAAAGA GAATGAGATG 
GACTGGGATG ACTCTGCCCT GACCATCACC GTGAACCCCA TGGAGACATA CGAGGACCAG 
CATAGCAGTG AGGAGGAAGA GGAGGAGGAG GAAGAGGAGA GCGAGGATGG GGACGAGGAG 
GACGACATCA CCAGTGCTGA GTCGGAGAGC AGTGAGGAGG ACGAGGGGGA GCACGGGGAC 
CCGCAGACCG CGAACCGGCA GCAGCAGCTG GAGTGGGACG ACTCCACCCT CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141646.1
CDS	60..2939
Translation	MLRRPAPALAPAAWLLLAGLLCSGGVWASRVNKHKPWLEPTYHGLVTESDSTVLLDPPLIALDKDAPLRFAGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSTEKLDCELQKDYTFTIQAYDCGKGPDGANVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVVEGKQYDSILRVEAVDADCSPQFSQICNYEIVTPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRAAEDVLVKISIKPTCTPGWQGWNNRVEYEPGTGALTLFPNVHLETCDESVASAQVVVELETSHIGKGCDRDTYSEKSLHRLCGAASGTAELLPSPGGSWNWTIGLPTDNGHDSDQVFEFNGTQAVRVPDSIVSVNPKEPFTISVWMRHGPFGKKKETIVCSSDKTEMNRHHYSLYVHGCRLVFLLRQDPSEEKKYKPAEFHWKLSQVCDEEWHHYVLNVEIPSVTLYVDGVPHEPFSVTEDYPLHPSKIETQLVVGACWQGGDLRMTQFFRGNLAGLTIRSGKLTDKKVIDCLYTCKEGLDLHTPEDNGRGVKIQANPTQSMLTLEGEDVGELDKAMQHISYLNSRQFPTPGIRRLKITSTVKCFNKATCISIPPVDGYIMVLQPEEPKISLSGIHHFARAASEFESSEGVFLFPELRIISTITREVEPEGEGDEDPTVQESLVSEEIVHDLDTCEVTVEGEELNGEQESLEVDAARLQQKGIEMSSSDMGVVFTGVDTMASYEEILHLLRYRNWHTRSLLDRKFKLVCSELNGRYVSNEFQVEVNVIHTANPMEHASHIAAQPQFVHPEHRSFIDLSGHNLANPHPFAAVLSPLAPVVPSTATVVIVVCVSFLVFMIILGVFRIRAAHQRTMRDQDTGKENEMDWDDSALTITVNPMETYEDQHSSEEEEEEEEEESEDGDEEDDITSAESESSEEDEGEHGDPQTANRQQQLEWDDSTLSY

Target ORF information:

RefSeq Version	XM_025285861.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis calsyntenin 1 (CLSTN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285861.1

ORF Insert Sequence:

ATGCTGCGCC GCCCTGCGCC CGCGCTGGCC CCTGCCGCCT GGCTGCTGCT GGCCGGGCTG 
CTCTGCAGCG GCGGGGTCTG GGCCTCGCGA GTCAACAAGC ACAAGCCGTG GCTGGAGCCC 
ACATATCATG GCTTAGTTAC GGAGAGCGAC AGCACTGTGC TCCTCGACCC CCCACTCATT 
GCCCTGGACA AAGATGCTCC TTTGCGCTTT GCAGGTGAGA TTTGTGGATT TAAGATTCAT 
GGACAGAATG TGCCCTTTGA CGCAGTGGTG GTGGATAAAT CCACTGGCGA GGGGGTCATT 
CGTTCCACAG AGAAGCTGGA CTGTGAGCTG CAGAAGGACT ACACGTTCAC CATCCAGGCC 
TATGACTGCG GGAAGGGGCC AGACGGGGCC AATGTGAAAA AATCTCACAA AGCAACTGTC 
CACATTCAGG TGAACGACGT CAACGAATAT GCGCCCGTGT TCAAGGAGAA GTCCTACAAA 
GCCACAGTGG TGGAGGGGAA GCAGTACGAC AGCATCTTGA GGGTGGAGGC GGTGGACGCA 
GACTGCTCCC CCCAGTTCAG CCAAATCTGC AACTATGAGA TCGTCACCCC GGATGTGCCA 
TTCACCGTTG ACAAAGATGG TTACATTAAA AACACGGAGA AGCTGAACTA TGGTAAAGAG 
CACCAGTACA AGCTGACTGT CACAGCCTAC GACTGCGGGA AGAAAAGAGC GGCAGAAGAC 
GTTTTGGTGA AGATCAGCAT CAAGCCCACC TGCACCCCTG GGTGGCAAGG ATGGAACAAC 
AGGGTGGAAT ACGAGCCGGG CACTGGTGCT CTGACACTCT TTCCGAATGT CCACCTGGAA 
ACGTGTGACG AGTCGGTGGC CTCGGCACAG GTGGTGGTGG AGCTGGAGAC CAGCCACATC 
GGGAAGGGTT GTGACCGTGA CACCTACTCC GAGAAGTCAC TTCACCGGCT CTGCGGTGCT 
GCGTCTGGCA CGGCTGAGCT GCTACCTTCC CCAGGAGGCT CCTGGAACTG GACTATTGGC 
CTTCCCACGG ACAACGGCCA TGACAGCGAC CAGGTCTTCG AGTTCAACGG CACTCAGGCA 
GTGAGGGTCC CAGACAGCAT CGTCTCTGTC AACCCCAAGG AGCCCTTTAC GATCTCTGTG 
TGGATGAGGC ATGGGCCATT TGGCAAGAAG AAGGAGACGA TTGTTTGCAG CTCAGACAAA 
ACAGAGATGA ACCGACACCA TTACTCACTC TATGTCCATG GCTGCCGGCT GGTTTTCCTC 
CTCCGTCAGG ATCCTTCAGA AGAGAAGAAA TACAAACCTG CAGAGTTCCA CTGGAAGCTG 
AGTCAGGTCT GTGACGAGGA ATGGCATCAC TACGTCCTCA ACGTGGAAAT CCCCAGTGTG 
ACTCTCTACG TCGACGGTGT CCCTCATGAG CCCTTCTCTG TCACTGAAGA TTACCCACTT 
CATCCATCCA AGATCGAAAC CCAGCTCGTG GTTGGGGCTT GCTGGCAAGG TGGTGACCTG 
CGTATGACCC AGTTTTTCCG AGGTAATCTG GCTGGCCTGA CCATCCGTTC TGGGAAGCTC 
ACAGATAAGA AGGTGATTGA CTGTCTGTAT ACCTGCAAAG AGGGGCTAGA CCTGCACACC 
CCCGAAGACA ATGGCAGAGG TGTCAAGATC CAGGCCAACC CTACTCAGTC GATGTTGACC 
TTGGAGGGAG AGGACGTGGG GGAGCTTGAT AAGGCCATGC AGCACATTTC CTACCTTAAT 
TCACGACAGT TCCCCACCCC AGGGATCCGA AGACTCAAAA TCACCAGCAC AGTCAAGTGT 
TTTAACAAGG CCACCTGCAT CTCCATCCCC CCGGTGGATG GCTACATAAT GGTTTTGCAG 
CCAGAAGAGC CCAAGATCAG TCTGAGCGGC ATCCACCATT TTGCTCGAGC GGCTTCCGAA 
TTTGAAAGCT CGGAGGGGGT TTTCCTCTTC CCCGAGCTTC GGATCATCAG CACCATCACG 
AGAGAAGTGG AACCCGAAGG GGAAGGGGAT GAGGACCCAA CAGTTCAAGA GTCACTGGTG 
TCAGAGGAGA TCGTGCACGA CCTGGACACC TGTGAGGTCA CAGTGGAGGG AGAAGAACTG 
AATGGAGAGC AGGAGAGCCT GGAGGTGGAC GCGGCCCGCC TGCAGCAGAA GGGCATCGAA 
ATGAGCAGCT CTGACATGGG CGTGGTCTTC ACAGGTGTTG ACACCATGGC CAGCTATGAG 
GAGATCTTAC ACCTACTGCG CTATCGGAAC TGGCACACCA GGTCCTTGCT TGACCGCAAG 
TTCAAGCTTG TCTGCTCTGA GCTGAATGGT CGCTATGTCA GCAACGAGTT CCAGGTGGAG 
GTGAATGTCA TCCACACAGC CAACCCCATG GAGCACGCCA GCCACATAGC TGCCCAGCCA 
CAGTTCGTCC ACCCCGAGCA CCGCTCCTTC ATTGACCTCT CGGGTCACAA CCTGGCCAAC 
CCCCACCCGT TTGCAGCCGT CCTGTCTCCT CTTGCCCCAG TCGTGCCCAG CACGGCCACC 
GTCGTCATCG TGGTGTGCGT CAGCTTCCTG GTTTTCATGA TCATCCTGGG GGTGTTCCGG 
ATCCGGGCTG CACACCAGAG AACCATGCGG GACCAGGACA CCGGGAAAGA GAATGAGATG 
GACTGGGATG ACTCTGCCCT GACCATCACC GTGAACCCCA TGGAGACATA CGAGGACCAG 
CATAGCAGTG AGGAGGAAGA GGAGGAGGAG GAAGAGGAGA GCGAGGATGG GGACGAGGAG 
GACGACATCA CCAGTGCTGA GTCGGAGAGC AGTGAGGAGG ACGAGGGGGA GCACGGGGAC 
CCGCAGACCG CGAACCGGCA GCAGCAGCTG GAGTGGGACG ACTCCACCCT CAGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLSTN1 ( XM_006076796.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006076858.1 Bubalus bubalis calsyntenin 1 (CLSTN1), mRNA.

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CloneID	OBu30264
Clone ID Related Accession (Same CDS sequence)	XM_006076796.1
Accession Version	XM_006076796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	calsyntenin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785703.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGT CCTCCACCCC TACCCCTAAC CCCCAGCAAG GCCTCGTCCG CCTGCAGCTC 
CAGGAGATCC TGGACCCACC TAACCCCACA CAGCACCCAA AGCCGCCCCC GCTGTTCCCT 
GACCTGGCTT GGCCACTCCT GGCCAGGCCT ACCATGCAGC CCCTGCGGTG CACAATCAGA 
GATGCTCTGC CCCCAGACTG CAGCCCACAG GAGGCCTGGG GCAGGTCCAG AGTCAACAAG 
CACAAGCCGT GGCTGGAGCC CACATATCAT GGCTTAGTTA CGGAGAGCGA CAGCACTGTG 
CTCCTCGACC CCCCACTCAT TGCCCTGGAC AAAGATGCTC CTTTGCGCTT TGCAGAGAGT 
TTTGAGGTGA CAGTCACCAA AGAAGGTGAG ATTTGTGGAT TTAAGATTCA TGGACAGAAT 
GTGCCCTTTG ACGCAGTGGT GGTGGATAAA TCCACTGGCG AGGGGGTCAT TCGTTCCACA 
GAGAAGCTGG ACTGTGAGCT GCAGAAGGAC TACACGTTCA CCATCCAGGC CTATGACTGC 
GGGAAGGGGC CAGACGGGGC CAATGTGAAA AAATCTCACA AAGCAACTGT CCACATTCAG 
GTGAACGACG TCAACGAATA TGCGCCCGTG TTCAAGGAGA AGTCCTACAA AGCCACAGTG 
GTGGAGGGGA AGCAGTACGA CAGCATCTTG AGGGTGGAGG CGGTGGACGC AGACTGCTCC 
CCCCAGTTCA GCCAAATCTG CAACTATGAG ATCGTCACCC CGGATGTGCC ATTCACCGTT 
GACAAAGATG GTTACATTAA AAACACGGAG AAGCTGAACT ATGGTAAAGA GCACCAGTAC 
AAGCTGACTG TCACAGCCTA CGACTGCGGG AAGAAAAGAG CGGCAGAAGA CGTTTTGGTG 
AAGATCAGCA TCAAGCCCAC CTGCACCCCT GGGTGGCAAG GATGGAACAA CAGGGTGGAA 
TACGAGCCGG GCACCGGCGC TCTGACACTC TTTCCGAATG TCCACCTGGA AACGTGTGAC 
GAGTCGGTGG CCTCGGCACA GGTGGTGGTG GAGCTGGAGA CCAGCCACAT CGGGAAGGGT 
TGTGACCGTG ACACCTACTC CGAGAAGTCA CTTCACCGGC TCTGCGGTGC TGCGTCTGGC 
ACGGCTGAGC TGCTACCTTC CCCAGGAGGC TCCTGGAACT GGACTATTGG CCTTCCCACG 
GACAACGGCC ATGACAGCGA CCAGGTCTTC GAGTTCAACG GCACTCAGGC AGTGAGGGTC 
CCAGACAGCA TCGTCTCTGT CAACCCCAAG GAGCCCTTTA CGATCTCTGT GTGGATGAGG 
CATGGGCCAT TTGGCAAGAA GAAGGAGACG ATTGTTTGCA GCTCAGACAA AACAGAGATG 
AACCGACACC ATTACTCACT CTATGTCCAT GGCTGCCGGC TGGTTTTCCT CCTCCGTCAG 
GATCCTTCAG AAGAGAAGAA ATACAAACCT GCAGAGTTCC ACTGGAAGCT GAGTCAGGTC 
TGTGACGAGG AATGGCATCA CTACGTCCTC AACGTGGAAA TCCCCAGTGT GACTCTCTAC 
GTCGACGGTG TCCCTCATGA GCCCTTCTCT GTCACTGAAG ATTACCCACT TCATCCATCC 
AAGATCGAAA CCCAGCTCGT GGTTGGGGCT TGCTGGCAAG AGTATTCAGG AGTTGAAAGT 
GACAATGAAA CTGAGCCTGT GCCTCTGGCC TCTGCAGGTG GTGACCTGCG TATGACCCAG 
TTTTTCCGAG GTAATCTGGC TGGCCTGACC ATCCGTTCTG GGAAGCTCAC AGATAAGAAG 
GTGATTGACT GTCTGTATAC CTGCAAAGAG GGGCTAGACC TGCACACCCC CGAAGACAAT 
GGCAGAGGTG TCAAGATCCA GGCCAACCCT ACTCAGTCGA TGTTGACCTT GGAGGGAGAG 
GACGTGGGGG AGCTTGATAA GGCCATGCAG CACATTTCCT ACCTTAATTC ACGACAGTTC 
CCCACCCCAG GGATCCGAAG ACTCAAAATC ACCAGCACAG TCAAGTGTTT TAACAAGGCC 
ACCTGCATCT CCATCCCCCC GGTGGATGGC TACATAATGG TTTTGCAGCC AGAAGAGCCC 
AAGATCAGTC TGAGCGGCAT CCACCATTTT GCTCGAGCGG CTTCCGAATT TGAAAGCTCG 
GAGGGGGTTT TCCTCTTCCC CGAGCTTCGG ATCATCAGCA CCATCACGAG AGAAGTGGAA 
CCCGAAGGGG AAGGGGATGA GGACCCAACA GTTCAAGAGT CACTGGTGTC AGAGGAGATC 
GTGCACGACC TGGACACCTG TGAGGTCACA GTGGAGGGAG AAGAACTGAA TGGAGAGCAG 
GAGAGCCTGG AGGTGGACGC GGCCCGCCTG CAGCAGAAGG GCATCGAAAT GAGCAGCTCT 
GACATGGGCG TGGTCTTCAC AGGTGTTGAC ACCATGGCCA GCTATGAGGA GATCTTACAC 
CTACTGCGCT ATCGGAACTG GCACACCAGG TCCTTGCTTG ACCGCAAGTT CAAGCTTGTC 
TGCTCTGAGC TGAATGGTCG CTATGTCAGC AACGAGTTCC AGGTGGAGGT GAATGTCATC 
CACACAGCCA ACCCCATGGA GCACGCCAGC CACATAGCTG CCCAGCCACA GTTCGTCCAC 
CCCGAGCACC GCTCCTTCAT TGACCTCTCG GGTCACAACC TGGCCAACCC CCACCCGTTT 
GCAGTCGTGC CCAGCACGGC CACCGTCGTC ATCGTGGTGT GCGTCAGCTT CCTGGTTTTC 
ATGATCATCC TGGGGGTGTT CCGGATCCGG GCTGCACACC AGAGAACCAT GCGGGACCAG 
GACACCGGGA AAGAGAATGA GATGGACTGG GATGACTCTG CCCTGACCAT CACCGTGAAC 
CCCATGGAGA CATACGAGGA CCAGCATAGC AGTGAGGAGG AAGAGGAGGA GGAGGAAGAG 
GAGAGCGAGG ATGGGGACGA GGAGGACGAC ATCACCAGTG CTGAGTCGGA GAGCAGTGAG 
GAGGACGAGG GGGAGCACGG GGACCCGCAG ACCGCGAACC GGCAGCAGCA GCTGGAGTGG 
GACGACTCCA CCCTCAGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006076858.1
CDS	1..3084
Translation	MMVSSTPTPNPQQGLVRLQLQEILDPPNPTQHPKPPPLFPDLAWPLLARPTMQPLRCTIRDALPPDCSPQEAWGRSRVNKHKPWLEPTYHGLVTESDSTVLLDPPLIALDKDAPLRFAESFEVTVTKEGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSTEKLDCELQKDYTFTIQAYDCGKGPDGANVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVVEGKQYDSILRVEAVDADCSPQFSQICNYEIVTPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRAAEDVLVKISIKPTCTPGWQGWNNRVEYEPGTGALTLFPNVHLETCDESVASAQVVVELETSHIGKGCDRDTYSEKSLHRLCGAASGTAELLPSPGGSWNWTIGLPTDNGHDSDQVFEFNGTQAVRVPDSIVSVNPKEPFTISVWMRHGPFGKKKETIVCSSDKTEMNRHHYSLYVHGCRLVFLLRQDPSEEKKYKPAEFHWKLSQVCDEEWHHYVLNVEIPSVTLYVDGVPHEPFSVTEDYPLHPSKIETQLVVGACWQEYSGVESDNETEPVPLASAGGDLRMTQFFRGNLAGLTIRSGKLTDKKVIDCLYTCKEGLDLHTPEDNGRGVKIQANPTQSMLTLEGEDVGELDKAMQHISYLNSRQFPTPGIRRLKITSTVKCFNKATCISIPPVDGYIMVLQPEEPKISLSGIHHFARAASEFESSEGVFLFPELRIISTITREVEPEGEGDEDPTVQESLVSEEIVHDLDTCEVTVEGEELNGEQESLEVDAARLQQKGIEMSSSDMGVVFTGVDTMASYEEILHLLRYRNWHTRSLLDRKFKLVCSELNGRYVSNEFQVEVNVIHTANPMEHASHIAAQPQFVHPEHRSFIDLSGHNLANPHPFAVVPSTATVVIVVCVSFLVFMIILGVFRIRAAHQRTMRDQDTGKENEMDWDDSALTITVNPMETYEDQHSSEEEEEEEEEESEDGDEEDDITSAESESSEEDEGEHGDPQTANRQQQLEWDDSTLSY

Target ORF information:

RefSeq Version	XM_006076796.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis calsyntenin 1 (CLSTN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076796.1

ORF Insert Sequence:

ATGATGGTGT CCTCCACCCC TACCCCTAAC CCCCAGCAAG GCCTCGTCCG CCTGCAGCTC 
CAGGAGATCC TGGACCCACC TAACCCCACA CAGCACCCAA AGCCGCCCCC GCTGTTCCCT 
GACCTGGCTT GGCCACTCCT GGCCAGGCCT ACCATGCAGC CCCTGCGGTG CACAATCAGA 
GATGCTCTGC CCCCAGACTG CAGCCCACAG GAGGCCTGGG GCAGGTCCAG AGTCAACAAG 
CACAAGCCGT GGCTGGAGCC CACATATCAT GGCTTAGTTA CGGAGAGCGA CAGCACTGTG 
CTCCTCGACC CCCCACTCAT TGCCCTGGAC AAAGATGCTC CTTTGCGCTT TGCAGAGAGT 
TTTGAGGTGA CAGTCACCAA AGAAGGTGAG ATTTGTGGAT TTAAGATTCA TGGACAGAAT 
GTGCCCTTTG ACGCAGTGGT GGTGGATAAA TCCACTGGCG AGGGGGTCAT TCGTTCCACA 
GAGAAGCTGG ACTGTGAGCT GCAGAAGGAC TACACGTTCA CCATCCAGGC CTATGACTGC 
GGGAAGGGGC CAGACGGGGC CAATGTGAAA AAATCTCACA AAGCAACTGT CCACATTCAG 
GTGAACGACG TCAACGAATA TGCGCCCGTG TTCAAGGAGA AGTCCTACAA AGCCACAGTG 
GTGGAGGGGA AGCAGTACGA CAGCATCTTG AGGGTGGAGG CGGTGGACGC AGACTGCTCC 
CCCCAGTTCA GCCAAATCTG CAACTATGAG ATCGTCACCC CGGATGTGCC ATTCACCGTT 
GACAAAGATG GTTACATTAA AAACACGGAG AAGCTGAACT ATGGTAAAGA GCACCAGTAC 
AAGCTGACTG TCACAGCCTA CGACTGCGGG AAGAAAAGAG CGGCAGAAGA CGTTTTGGTG 
AAGATCAGCA TCAAGCCCAC CTGCACCCCT GGGTGGCAAG GATGGAACAA CAGGGTGGAA 
TACGAGCCGG GCACCGGCGC TCTGACACTC TTTCCGAATG TCCACCTGGA AACGTGTGAC 
GAGTCGGTGG CCTCGGCACA GGTGGTGGTG GAGCTGGAGA CCAGCCACAT CGGGAAGGGT 
TGTGACCGTG ACACCTACTC CGAGAAGTCA CTTCACCGGC TCTGCGGTGC TGCGTCTGGC 
ACGGCTGAGC TGCTACCTTC CCCAGGAGGC TCCTGGAACT GGACTATTGG CCTTCCCACG 
GACAACGGCC ATGACAGCGA CCAGGTCTTC GAGTTCAACG GCACTCAGGC AGTGAGGGTC 
CCAGACAGCA TCGTCTCTGT CAACCCCAAG GAGCCCTTTA CGATCTCTGT GTGGATGAGG 
CATGGGCCAT TTGGCAAGAA GAAGGAGACG ATTGTTTGCA GCTCAGACAA AACAGAGATG 
AACCGACACC ATTACTCACT CTATGTCCAT GGCTGCCGGC TGGTTTTCCT CCTCCGTCAG 
GATCCTTCAG AAGAGAAGAA ATACAAACCT GCAGAGTTCC ACTGGAAGCT GAGTCAGGTC 
TGTGACGAGG AATGGCATCA CTACGTCCTC AACGTGGAAA TCCCCAGTGT GACTCTCTAC 
GTCGACGGTG TCCCTCATGA GCCCTTCTCT GTCACTGAAG ATTACCCACT TCATCCATCC 
AAGATCGAAA CCCAGCTCGT GGTTGGGGCT TGCTGGCAAG AGTATTCAGG AGTTGAAAGT 
GACAATGAAA CTGAGCCTGT GCCTCTGGCC TCTGCAGGTG GTGACCTGCG TATGACCCAG 
TTTTTCCGAG GTAATCTGGC TGGCCTGACC ATCCGTTCTG GGAAGCTCAC AGATAAGAAG 
GTGATTGACT GTCTGTATAC CTGCAAAGAG GGGCTAGACC TGCACACCCC CGAAGACAAT 
GGCAGAGGTG TCAAGATCCA GGCCAACCCT ACTCAGTCGA TGTTGACCTT GGAGGGAGAG 
GACGTGGGGG AGCTTGATAA GGCCATGCAG CACATTTCCT ACCTTAATTC ACGACAGTTC 
CCCACCCCAG GGATCCGAAG ACTCAAAATC ACCAGCACAG TCAAGTGTTT TAACAAGGCC 
ACCTGCATCT CCATCCCCCC GGTGGATGGC TACATAATGG TTTTGCAGCC AGAAGAGCCC 
AAGATCAGTC TGAGCGGCAT CCACCATTTT GCTCGAGCGG CTTCCGAATT TGAAAGCTCG 
GAGGGGGTTT TCCTCTTCCC CGAGCTTCGG ATCATCAGCA CCATCACGAG AGAAGTGGAA 
CCCGAAGGGG AAGGGGATGA GGACCCAACA GTTCAAGAGT CACTGGTGTC AGAGGAGATC 
GTGCACGACC TGGACACCTG TGAGGTCACA GTGGAGGGAG AAGAACTGAA TGGAGAGCAG 
GAGAGCCTGG AGGTGGACGC GGCCCGCCTG CAGCAGAAGG GCATCGAAAT GAGCAGCTCT 
GACATGGGCG TGGTCTTCAC AGGTGTTGAC ACCATGGCCA GCTATGAGGA GATCTTACAC 
CTACTGCGCT ATCGGAACTG GCACACCAGG TCCTTGCTTG ACCGCAAGTT CAAGCTTGTC 
TGCTCTGAGC TGAATGGTCG CTATGTCAGC AACGAGTTCC AGGTGGAGGT GAATGTCATC 
CACACAGCCA ACCCCATGGA GCACGCCAGC CACATAGCTG CCCAGCCACA GTTCGTCCAC 
CCCGAGCACC GCTCCTTCAT TGACCTCTCG GGTCACAACC TGGCCAACCC CCACCCGTTT 
GCAGTCGTGC CCAGCACGGC CACCGTCGTC ATCGTGGTGT GCGTCAGCTT CCTGGTTTTC 
ATGATCATCC TGGGGGTGTT CCGGATCCGG GCTGCACACC AGAGAACCAT GCGGGACCAG 
GACACCGGGA AAGAGAATGA GATGGACTGG GATGACTCTG CCCTGACCAT CACCGTGAAC 
CCCATGGAGA CATACGAGGA CCAGCATAGC AGTGAGGAGG AAGAGGAGGA GGAGGAAGAG 
GAGAGCGAGG ATGGGGACGA GGAGGACGAC ATCACCAGTG CTGAGTCGGA GAGCAGTGAG 
GAGGACGAGG GGGAGCACGG GGACCCGCAG ACCGCGAACC GGCAGCAGCA GCTGGAGTGG 
GACGACTCCA CCCTCAGCTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CLSTN1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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