PIWIL2 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following PIWIL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIWIL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu06442 XM_006047911.1
Latest version!
Bubalus bubalis piwi-like RNA-mediated gene silencing 2 (PIWIL2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBu63020 XM_025281861.1
Latest version!
Bubalus bubalis piwi like RNA-mediated gene silencing 2 (PIWIL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu63020 XM_006047911.2
Latest version!
Bubalus bubalis piwi like RNA-mediated gene silencing 2 (PIWIL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBu63020 XM_025281862.1
Latest version!
Bubalus bubalis piwi like RNA-mediated gene silencing 2 (PIWIL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu06442
    Clone ID Related Accession (Same CDS sequence) XM_006047911.1
    Accession Version XM_006047911.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product piwi-like protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784071.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTTTGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    CCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGCCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006047973.1
    CDS123..3032
    Translation

    Target ORF information:

    RefSeq Version XM_006047911.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis piwi-like RNA-mediated gene silencing 2 (PIWIL2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006047911.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTTTGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    CCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGCCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu63020
    Clone ID Related Accession (Same CDS sequence) XM_025281861.1 , XM_006047911.2 , XM_025281862.1
    Accession Version XM_025281861.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product piwi-like protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTATGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    TCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGTCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025137646.1
    CDS204..3113
    Translation

    Target ORF information:

    RefSeq Version XM_025281861.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis piwi like RNA-mediated gene silencing 2 (PIWIL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025281861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTATGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    TCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGTCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu63020
    Clone ID Related Accession (Same CDS sequence) XM_025281861.1 , XM_006047911.2 , XM_025281862.1
    Accession Version XM_006047911.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product piwi-like protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047911.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTATGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    TCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGTCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006047973.2
    CDS178..3087
    Translation

    Target ORF information:

    RefSeq Version XM_006047911.2
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis piwi like RNA-mediated gene silencing 2 (PIWIL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006047911.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTATGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    TCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGTCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu63020
    Clone ID Related Accession (Same CDS sequence) XM_025281861.1 , XM_006047911.2 , XM_025281862.1
    Accession Version XM_025281862.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product piwi-like protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037547.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTATGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    TCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGTCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025137647.1
    CDS173..3082
    Translation

    Target ORF information:

    RefSeq Version XM_025281862.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis piwi like RNA-mediated gene silencing 2 (PIWIL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025281862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGATCCTG TCCGACCACG CTTCAGGGGC CTGTCTCCCG TGCACCCATC TCAGTCTGTG 
    CGGATGCCAG GCTCTTGGCC ACAAGCTCCA AAACCTATGG ACCCAGCTCT GAGCAGTGCA
    GTACCTGCAG GCAGGGGCCA TGTGTTTGGA AAGCAAAAGG AGCCGAGTCC ACAGAGTGGG
    TCAGTGCAAA AGGAGTCTGT GGGTTTAGTC TCAATGTTCC GAGGACTGGG GCTTGAAACA
    GCATCCCAGA CCCCTCCGAA ACGGGAGATG CCTCCTTTAG GTAGAGGCAT TTTAGGTCTA
    GGCTTATCTT CCAGTATGGC ATGCAAGGAC AAAGAAGAAC CCCATCCCAC TCTTCTGGAT
    CCTTCATTGT TGGCAGCTGG GGATAGCAAG ATGGCAGAGG CCTCCGTTGG TTGGAATAGG
    ATGCTCGGAA GAGGGAATTT GGATGCATCT TTGTTACCAG TGGGAAGAGC AGCTGGTTTA
    GGCAGAGGAA TATACAAGGC TCCCGGTGCT CTCAGCCCGC CTTATCAGGA TCCTGCCCAG
    CTCTCACCTC TGCCCGCTCT GCCCCAGGCC CCACTATACT CTCTTGATAC CCCTCCACTT
    GGGACTGCAG AGCGCAAGAA AGAGCTTTTT GTGAAGCAAG GATCAAAAGG AATACCTCAG
    TCTTTGGGAT TGAACCTTAT CAAAATACAG TGTCATAATG AAGCAGTTTA TCAATATCAT
    GTGACTTTCA GCCCCAACGT GGAGTGCAAA AGCATGAGGT TTGGCATGTT GAAGGACCAT
    CAAGCTGTCA CCGGAAATGT CACTGCTTTT GATGGATCCA TTCTCTATCT ACCTGTTAGG
    CTTCAGCAAG TTCTTGAGTT AAAGAGTCAA AGGAAAACTG ACAGTGCTGA GATCACCATT
    AAGATTCAGT TGACAAAGAT CCTGGAGCCC TGTTCTGACT TGTGCATTCC CTTCTACAAT
    GTTGTATTCC GTCGGGTAAT GAAACTTTTA GATATGAAGC TTGTGGGAAG AAACTTCTAT
    GACCCTACAA GTGCTATGGT GCTGCAGCAA CACAGATTGC AGATCTGGCC GGGCTATGCA
    GCTAGCATCC GGAGGACAGA CGGAGGTCTT TTCCTGCTAG CGGATGTCTC CCATAAGGTC
    ATTCGGAATG ATTCTGTGCT GGACATCATG CACACCATGT ACTTGCAGAA CAAAGAAAAC
    TTCCAGGACG AGTGCACTAA GCTTCTGGTT GGCAATATTG TTATAACCCG ATACAACAAT
    CGTACCTATC GTATTGATGA TGTGGATTGG AATAAGACTC CAAAAGATAG CTTCACCATG
    TCTGATGGGA AGGAGATTAC ATTCTTGGAC TACTACAGCA AAAACTATGG GATCACAGTA
    AAGGAAGAGG ACCAGCCACT GCTGATCCAC AGGCCCAGTG AGAGACAGAA TAACCAAGGG
    ATGTTGCTAA AAAGTGAAAT CCTGCTGCTC CCTGAGCTTT CCTTCATGAC TGGAATCCCT
    GAGAAGATGA AGAAGGACTT CAGAGCAATG AAGGATTTGA CCCAGCAGAT CAACCTGAGC
    CCCAAACAGC ACCATAGTGC TTTGGAATGC TTGCTGCAGA GAATTTCGAA AAATGAGACA
    GCTAACAGTG AACTGACACG TTGGGGACTC TATCTGCAGA AAGATGTACA TAAGATTGAA
    GGACGTGTTC TGCCAATGGA AAGAATTAAC TTAAGAAATA TATCATTTAT CACATCTCCG
    GAACTAAACT GGATTAAGGA AGTAACCAGA GACCTTTCCA TCTTGACTGT CCCCTTGCAT
    TTCTGGGCAC TGTTTTACCC AAAGAGAGCA ATGGATCAAG CCCGAGAGCT GGTGAACATG
    TTGGAGAAGA TAGCCGGCCC CATTGGCATG CGTATGAGCC CACCGGCCTG GGTGGAACTG
    AAGGATGATC GCATAGAGAC CTATGTCAGA ACCATTCAAT CCATGCTGGG GGTTGAGGGG
    AAGATACAGA TGGTTGTTTG CATCATCATG GGTACACGTG ATGATCTCTA TGGGGCCATT
    AAAAAGCTGT GCTGTGTGCA GACTCCTGTA CCCTCACAGG TCATCAATGT CCGAACCATT
    GGTCAGCCCA CCAGGCTTCG AAGTGTGGCC CAGAAAATTT TACTTCAGAT TAACTGTAAA
    TTGGGTGGTG AGCTCTGGGG AGTGGATATT CCTCTGAAAC AGTTAATGGT GATTGGAATG
    GATGTTTACC ATGACCCCAG CAGAGGCATG CGCTCTGTGG TTGGCTTCGT CGCAAGCATC
    AATCTCACCC TCACAAAATG GTATTCACGA GTGGTATTCC AGATGCCTCA TCAGGAGATT
    GTGGATAGCC TGAAGCTATG CCTGGTGGGC TCCTTAAAGA AGTTTTATGA GGTGAACCAC
    TGCCTCCCAG ATAAGATTGT GGTGTACCGT GATGGAGTAT CTGATGGCCA ACTAAAGACA
    GTTGCCAGCT ATGAAATTCC TCAGCTACAG AAGTGTTTTG AAGCTTTTGA GAATTATCAT
    CCCAAGATGG TGGTGTTTGT AGTGCAGAAG AAAATCAGCA CCAACCTGTA CCTGGCGGCC
    ACTGAGAACT TTGCGACTCC CTCCCCTGGG ACTGTGGTAG ATCATACGAT AACAAGCTGT
    GAGTGGGTGG ACTTCTACCT TCTTGCCCAT CATGTGCGGC AGGGTTGTGG CATTCCAACC
    CATTATGTTT GTGTTCTCAA CACTGCAAAC CTGAGCCCTG ATCATATGCA GAGGTTGACT
    TTCAAACTGT GCCACATGTA CTGGAATTGG CCTGGTACCA TTAGAGTTCC AGCTCCTTGC
    AAGTATGCCC ACAAGCTAGC CTTCCTGTCA GGACAGATCT TGCACCACGA ACCAGCCATC
    CAGCTGTGTG AGAACCTGTT CTTCCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.