HLTF cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	HLTF
Entrez Gene ID	102405523
Full Name	helicase like transcription factor
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006063126.1	XP_006063188.1	helicase-like transcription factor isoform X1
XM_006063127.2	XP_006063189.1	helicase-like transcription factor isoform X2
XM_006063128.1	XP_006063190.1	helicase-like transcription factor isoform X3
XM_006063126.2	XP_006063188.1	helicase-like transcription factor isoform X1
XM_006063127.1	XP_006063189.1	helicase-like transcription factor isoform X2

Pathways from BioSystems
Gene Ontology

The following HLTF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HLTF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu19097	XM_006063126.2 Latest version!	Bubalus bubalis helicase like transcription factor (HLTF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu19098	XM_006063127.2 Latest version!	Bubalus bubalis helicase like transcription factor (HLTF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu19099	XM_006063128.1 Latest version!	Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBu19097	XM_006063126.1	Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu19098	XM_006063127.1	Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HLTF ( XM_006063126.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006063188.1 Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu19097
Clone ID Related Accession (Same CDS sequence)	XM_006063126.1 , XM_006063126.2
Accession Version	XM_006063126.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	helicase-like transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGCAGGA TCTTCTAAGG TTCAAGAAGA TGCAGAATTT 
GCATGTGCAC TTACTTCATC TACCCCTGTA ACAAAAAAGA AAATGTTGAA AAGGGGGGCG 
TCTGCAGTGG AGAGTTCAAA GAAAACTGAT GTTGAAGATA AACCAAGAAC AACACTGATC 
ATCTGTCCAC TTTCTGTATT AAGCAACTGG ATGGACCAGT TTGGACAACA CATAAAATCA 
GATGTGCACT TGAATTTTTA TGTTTATTAT GGTCCTGATC GTGTTAGAGA CCCAACATTG 
CTTTCAAAAC AGGATATTGT TTTGACGACA TACAATATTT TAACTCATGA CTATGGTACT 
AAGGGTGATA GTCCATTACA TAGCATAAGG TGGCTAAGAG TGATCCTGGA TGAAGGACAT 
GCCATACGGA ATCCAAATGC TCAGCAAACA AAAGCTGTAC TTGATTTAGA AGCAGAAAGA 
AGATGGGTTT TGACAGGCAC TCCAATTCAG AATTCTTTAA AGGACTTGTG GTCTCTTCTT 
TCCTTTTTAA AACTTAAACC ATTTGTTGAT AGAGAATGGT GGCATAGAAC AATACAGCGT 
CCTGTCACAA TGGGAGACGA AGCTGGACTT AGGCGTTTGC AGTCCCTAAT TAAAAATATT 
ACACTTAGAA GAACAAAGAC AAGCAAAATT AAAGGAAAAC CTGTTTTGGA GTTACCAGAA 
CGTAAAGTAT TTATTCAGCA CATTACACTT TCAGATGAAG AGAGAAAGAT TTACCAGTCT 
GTGAAAAATG AAGGCAAAGC TACTATTGGA AGGTATTTTA ATGAAGGGAC TGTCCTTGCA 
CACTATGCAG ATGTCCTGGG TCTTTTGCTT AGATTGCGGC AGATTTGTTG TCATACACAC 
CTTCTTGCAA ATGCAGTGTC TTCCAGTGGT CCCTCAGGGA ATGATACACC TGAAGAACTA 
CGAAAGAAGT TAATAAAGAA GATGAAGTTA ATTCTAAGCT CAGGTTCTGA TGAAGAATGT 
GCAATTTGCT TGGATTCTTT AATGGCCCCT GTCATAACAC ATTGTGCACA TGTCTTCTGT 
AAACCTTGTA TTTGCCAAGT CATTCAGAAT GAGCAGCCTC ATGCTAAATG CCCTTTATGC 
AGAAATGATA TACATGGAGA CAGTTTAATA GAATGTCCTC CAGAAGAATT GGCATGCAAC 
ACTGAGAAAA AGTCTAATAT GGAATGGACA TCCAGTTCGA AGATTAATGC TCTAATGCAT 
GCATTGATTG ATTTAAGAAA GAAGAACCCC AACATAAAAA GTTTAGTTGT TTCTCAGTTT 
ACAACATTTC TGTCTTTAAT AGAAACACCC CTCAGATCCT CTGGATTTGT ATTTACTCGT 
TTGGATGGTT CCATGGCCCA AAAAAAAAGA GTTGAATCAA TTCAGTGTTT TCAAAACACT 
GAAGCAGGGT CTCCAACTAT AATGCTTCTA TCCTTAAAAG CAGGTGGAGT TGGTTTGAAT 
CTTTCTGCAG CTTCTCGAGT TTTTTTAATG GATCCAGCCT GGAATCCAGC TGCTGAAGAT 
CAGTGCTTTG ACAGATGCCA TAGACTTGGC CAGAAGCAAG AAGTTATTAT CACAAAATTC 
ATTGTGAAGG ATTCTGTTGA AGAAAACATG CTGAAAATAC AAAACACGAA GAGAGAACTT 
GCAGCTGGAG CCTTTGGAAC TAAGAAAACA AATGCGAGTG AAATGAAACA AGCTAAAATC 
AATGAAATCA GAACTTTAAT TGATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006063188.1
CDS	203..3232
Translation	MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDIIPPDDFLTSDEELDSVLFGTLRGHVVGLRYYTGVVNNNEMVALQREPTNPYDKNAIKVNNVNGNQVGHIKKDLAAALAYIMDNKLAQIEGVVPFGANNTFTMPLQMTFWGKEENRKAVLDELKKHGFKLGPAPKSLGFSLESGWGSGRAGPSYSMPVHTAVQMTTEQLKTEFDKLFEDLKEDDKTQEMEPAEAIETPLLPHQKQALAWMVSRENSEELPPFWEQRSDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGKSLPVERIKKNQLKKECNVYDESMELGGNNTSEKAAGLIKGSRCSGEPSISDVKGKNKHPKSEFSSSRSKRRKPAAVQYTESSDSEEVEPSELPQKMKGKLKRTQSETKIRVKAGSSKVQEDAEFACALTSSTPVTKKKMLKRGASAVESSKKTDVEDKPRTTLIICPLSVLSNWMDQFGQHIKSDVHLNFYVYYGPDRVRDPTLLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFVDREWWHRTIQRPVTMGDEAGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGKATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTHLLANAVSSSGPSGNDTPEELRKKLIKKMKLILSSGSDEECAICLDSLMAPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHGDSLIECPPEELACNTEKKSNMEWTSSSKINALMHALIDLRKKNPNIKSLVVSQFTTFLSLIETPLRSSGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNTKRELAAGAFGTKKTNASEMKQAKINEIRTLIDL

Target ORF information:

RefSeq Version	XM_006063126.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006063126.1

ORF Insert Sequence:

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGCAGGA TCTTCTAAGG TTCAAGAAGA TGCAGAATTT 
GCATGTGCAC TTACTTCATC TACCCCTGTA ACAAAAAAGA AAATGTTGAA AAGGGGGGCG 
TCTGCAGTGG AGAGTTCAAA GAAAACTGAT GTTGAAGATA AACCAAGAAC AACACTGATC 
ATCTGTCCAC TTTCTGTATT AAGCAACTGG ATGGACCAGT TTGGACAACA CATAAAATCA 
GATGTGCACT TGAATTTTTA TGTTTATTAT GGTCCTGATC GTGTTAGAGA CCCAACATTG 
CTTTCAAAAC AGGATATTGT TTTGACGACA TACAATATTT TAACTCATGA CTATGGTACT 
AAGGGTGATA GTCCATTACA TAGCATAAGG TGGCTAAGAG TGATCCTGGA TGAAGGACAT 
GCCATACGGA ATCCAAATGC TCAGCAAACA AAAGCTGTAC TTGATTTAGA AGCAGAAAGA 
AGATGGGTTT TGACAGGCAC TCCAATTCAG AATTCTTTAA AGGACTTGTG GTCTCTTCTT 
TCCTTTTTAA AACTTAAACC ATTTGTTGAT AGAGAATGGT GGCATAGAAC AATACAGCGT 
CCTGTCACAA TGGGAGACGA AGCTGGACTT AGGCGTTTGC AGTCCCTAAT TAAAAATATT 
ACACTTAGAA GAACAAAGAC AAGCAAAATT AAAGGAAAAC CTGTTTTGGA GTTACCAGAA 
CGTAAAGTAT TTATTCAGCA CATTACACTT TCAGATGAAG AGAGAAAGAT TTACCAGTCT 
GTGAAAAATG AAGGCAAAGC TACTATTGGA AGGTATTTTA ATGAAGGGAC TGTCCTTGCA 
CACTATGCAG ATGTCCTGGG TCTTTTGCTT AGATTGCGGC AGATTTGTTG TCATACACAC 
CTTCTTGCAA ATGCAGTGTC TTCCAGTGGT CCCTCAGGGA ATGATACACC TGAAGAACTA 
CGAAAGAAGT TAATAAAGAA GATGAAGTTA ATTCTAAGCT CAGGTTCTGA TGAAGAATGT 
GCAATTTGCT TGGATTCTTT AATGGCCCCT GTCATAACAC ATTGTGCACA TGTCTTCTGT 
AAACCTTGTA TTTGCCAAGT CATTCAGAAT GAGCAGCCTC ATGCTAAATG CCCTTTATGC 
AGAAATGATA TACATGGAGA CAGTTTAATA GAATGTCCTC CAGAAGAATT GGCATGCAAC 
ACTGAGAAAA AGTCTAATAT GGAATGGACA TCCAGTTCGA AGATTAATGC TCTAATGCAT 
GCATTGATTG ATTTAAGAAA GAAGAACCCC AACATAAAAA GTTTAGTTGT TTCTCAGTTT 
ACAACATTTC TGTCTTTAAT AGAAACACCC CTCAGATCCT CTGGATTTGT ATTTACTCGT 
TTGGATGGTT CCATGGCCCA AAAAAAAAGA GTTGAATCAA TTCAGTGTTT TCAAAACACT 
GAAGCAGGGT CTCCAACTAT AATGCTTCTA TCCTTAAAAG CAGGTGGAGT TGGTTTGAAT 
CTTTCTGCAG CTTCTCGAGT TTTTTTAATG GATCCAGCCT GGAATCCAGC TGCTGAAGAT 
CAGTGCTTTG ACAGATGCCA TAGACTTGGC CAGAAGCAAG AAGTTATTAT CACAAAATTC 
ATTGTGAAGG ATTCTGTTGA AGAAAACATG CTGAAAATAC AAAACACGAA GAGAGAACTT 
GCAGCTGGAG CCTTTGGAAC TAAGAAAACA AATGCGAGTG AAATGAAACA AGCTAAAATC 
AATGAAATCA GAACTTTAAT TGATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLTF ( XM_006063126.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006063188.1 Bubalus bubalis helicase like transcription factor (HLTF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu19097
Clone ID Related Accession (Same CDS sequence)	XM_006063126.1 , XM_006063126.2
Accession Version	XM_006063126.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	helicase-like transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006063126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGCAGGA TCTTCTAAGG TTCAAGAAGA TGCAGAATTT 
GCATGTGCAC TTACTTCATC TACCCCTGTA ACAAAAAAGA AAATGTTGAA AAGGGGGGCG 
TCTGCAGTGG AGAGTTCAAA GAAAACTGAT GTTGAAGATA AACCAAGAAC AACACTGATC 
ATCTGTCCAC TTTCTGTATT AAGCAACTGG ATGGACCAGT TTGGACAACA CATAAAATCA 
GATGTGCACT TGAATTTTTA TGTTTATTAT GGTCCTGATC GTGTTAGAGA CCCAACATTG 
CTTTCAAAAC AGGATATTGT TTTGACGACA TACAATATTT TAACTCATGA CTATGGTACT 
AAGGGTGATA GTCCATTACA TAGCATAAGG TGGCTAAGAG TGATCCTGGA TGAAGGACAT 
GCCATACGGA ATCCAAATGC TCAGCAAACA AAAGCTGTAC TTGATTTAGA AGCAGAAAGA 
AGATGGGTTT TGACAGGCAC TCCAATTCAG AATTCTTTAA AGGACTTGTG GTCTCTTCTT 
TCCTTTTTAA AACTTAAACC ATTTGTTGAT AGAGAATGGT GGCATAGAAC AATACAGCGT 
CCTGTCACAA TGGGAGACGA AGCTGGACTT AGGCGTTTGC AGTCCCTAAT TAAAAATATT 
ACACTTAGAA GAACAAAGAC AAGCAAAATT AAAGGAAAAC CTGTTTTGGA GTTACCAGAA 
CGTAAAGTAT TTATTCAGCA CATTACACTT TCAGATGAAG AGAGAAAGAT TTACCAGTCT 
GTGAAAAATG AAGGCAAAGC TACTATTGGA AGGTATTTTA ATGAAGGGAC TGTCCTTGCA 
CACTATGCAG ATGTCCTGGG TCTTTTGCTT AGATTGCGGC AGATTTGTTG TCATACACAC 
CTTCTTGCAA ATGCAGTGTC TTCCAGTGGT CCCTCAGGGA ATGATACACC TGAAGAACTA 
CGAAAGAAGT TAATAAAGAA GATGAAGTTA ATTCTAAGCT CAGGTTCTGA TGAAGAATGT 
GCAATTTGCT TGGATTCTTT AATGGCCCCT GTCATAACAC ATTGTGCACA TGTCTTCTGT 
AAACCTTGTA TTTGCCAAGT CATTCAGAAT GAGCAGCCTC ATGCTAAATG CCCTTTATGC 
AGAAATGATA TACATGGAGA CAGTTTAATA GAATGTCCTC CAGAAGAATT GGCATGCAAC 
ACTGAGAAAA AGTCTAATAT GGAATGGACA TCCAGTTCGA AGATTAATGC TCTAATGCAT 
GCATTGATTG ATTTAAGAAA GAAGAACCCC AACATAAAAA GTTTAGTTGT TTCTCAGTTT 
ACAACATTTC TGTCTTTAAT AGAAACACCC CTCAGATCCT CTGGATTTGT ATTTACTCGT 
TTGGATGGTT CCATGGCCCA AAAAAAAAGA GTTGAATCAA TTCAGTGTTT TCAAAACACT 
GAAGCAGGGT CTCCAACTAT AATGCTTCTA TCCTTAAAAG CAGGTGGAGT TGGTTTGAAT 
CTTTCTGCAG CTTCTCGAGT TTTTTTAATG GATCCAGCCT GGAATCCAGC TGCTGAAGAT 
CAGTGCTTTG ACAGATGCCA TAGACTTGGC CAGAAGCAAG AAGTTATTAT CACAAAATTC 
ATTGTGAAGG ATTCTGTTGA AGAAAACATG CTGAAAATAC AAAACACGAA GAGAGAACTT 
GCAGCTGGAG CCTTTGGAAC TAAGAAAACA AATGCGAGTG AAATGAAACA AGCTAAAATC 
AATGAAATCA GAACTTTAAT TGATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006063188.1
CDS	215..3244
Translation	MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDIIPPDDFLTSDEELDSVLFGTLRGHVVGLRYYTGVVNNNEMVALQREPTNPYDKNAIKVNNVNGNQVGHIKKDLAAALAYIMDNKLAQIEGVVPFGANNTFTMPLQMTFWGKEENRKAVLDELKKHGFKLGPAPKSLGFSLESGWGSGRAGPSYSMPVHTAVQMTTEQLKTEFDKLFEDLKEDDKTQEMEPAEAIETPLLPHQKQALAWMVSRENSEELPPFWEQRSDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGKSLPVERIKKNQLKKECNVYDESMELGGNNTSEKAAGLIKGSRCSGEPSISDVKGKNKHPKSEFSSSRSKRRKPAAVQYTESSDSEEVEPSELPQKMKGKLKRTQSETKIRVKAGSSKVQEDAEFACALTSSTPVTKKKMLKRGASAVESSKKTDVEDKPRTTLIICPLSVLSNWMDQFGQHIKSDVHLNFYVYYGPDRVRDPTLLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFVDREWWHRTIQRPVTMGDEAGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGKATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTHLLANAVSSSGPSGNDTPEELRKKLIKKMKLILSSGSDEECAICLDSLMAPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHGDSLIECPPEELACNTEKKSNMEWTSSSKINALMHALIDLRKKNPNIKSLVVSQFTTFLSLIETPLRSSGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNTKRELAAGAFGTKKTNASEMKQAKINEIRTLIDL

Target ORF information:

RefSeq Version	XM_006063126.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis helicase like transcription factor (HLTF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006063126.2

ORF Insert Sequence:

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGCAGGA TCTTCTAAGG TTCAAGAAGA TGCAGAATTT 
GCATGTGCAC TTACTTCATC TACCCCTGTA ACAAAAAAGA AAATGTTGAA AAGGGGGGCG 
TCTGCAGTGG AGAGTTCAAA GAAAACTGAT GTTGAAGATA AACCAAGAAC AACACTGATC 
ATCTGTCCAC TTTCTGTATT AAGCAACTGG ATGGACCAGT TTGGACAACA CATAAAATCA 
GATGTGCACT TGAATTTTTA TGTTTATTAT GGTCCTGATC GTGTTAGAGA CCCAACATTG 
CTTTCAAAAC AGGATATTGT TTTGACGACA TACAATATTT TAACTCATGA CTATGGTACT 
AAGGGTGATA GTCCATTACA TAGCATAAGG TGGCTAAGAG TGATCCTGGA TGAAGGACAT 
GCCATACGGA ATCCAAATGC TCAGCAAACA AAAGCTGTAC TTGATTTAGA AGCAGAAAGA 
AGATGGGTTT TGACAGGCAC TCCAATTCAG AATTCTTTAA AGGACTTGTG GTCTCTTCTT 
TCCTTTTTAA AACTTAAACC ATTTGTTGAT AGAGAATGGT GGCATAGAAC AATACAGCGT 
CCTGTCACAA TGGGAGACGA AGCTGGACTT AGGCGTTTGC AGTCCCTAAT TAAAAATATT 
ACACTTAGAA GAACAAAGAC AAGCAAAATT AAAGGAAAAC CTGTTTTGGA GTTACCAGAA 
CGTAAAGTAT TTATTCAGCA CATTACACTT TCAGATGAAG AGAGAAAGAT TTACCAGTCT 
GTGAAAAATG AAGGCAAAGC TACTATTGGA AGGTATTTTA ATGAAGGGAC TGTCCTTGCA 
CACTATGCAG ATGTCCTGGG TCTTTTGCTT AGATTGCGGC AGATTTGTTG TCATACACAC 
CTTCTTGCAA ATGCAGTGTC TTCCAGTGGT CCCTCAGGGA ATGATACACC TGAAGAACTA 
CGAAAGAAGT TAATAAAGAA GATGAAGTTA ATTCTAAGCT CAGGTTCTGA TGAAGAATGT 
GCAATTTGCT TGGATTCTTT AATGGCCCCT GTCATAACAC ATTGTGCACA TGTCTTCTGT 
AAACCTTGTA TTTGCCAAGT CATTCAGAAT GAGCAGCCTC ATGCTAAATG CCCTTTATGC 
AGAAATGATA TACATGGAGA CAGTTTAATA GAATGTCCTC CAGAAGAATT GGCATGCAAC 
ACTGAGAAAA AGTCTAATAT GGAATGGACA TCCAGTTCGA AGATTAATGC TCTAATGCAT 
GCATTGATTG ATTTAAGAAA GAAGAACCCC AACATAAAAA GTTTAGTTGT TTCTCAGTTT 
ACAACATTTC TGTCTTTAAT AGAAACACCC CTCAGATCCT CTGGATTTGT ATTTACTCGT 
TTGGATGGTT CCATGGCCCA AAAAAAAAGA GTTGAATCAA TTCAGTGTTT TCAAAACACT 
GAAGCAGGGT CTCCAACTAT AATGCTTCTA TCCTTAAAAG CAGGTGGAGT TGGTTTGAAT 
CTTTCTGCAG CTTCTCGAGT TTTTTTAATG GATCCAGCCT GGAATCCAGC TGCTGAAGAT 
CAGTGCTTTG ACAGATGCCA TAGACTTGGC CAGAAGCAAG AAGTTATTAT CACAAAATTC 
ATTGTGAAGG ATTCTGTTGA AGAAAACATG CTGAAAATAC AAAACACGAA GAGAGAACTT 
GCAGCTGGAG CCTTTGGAAC TAAGAAAACA AATGCGAGTG AAATGAAACA AGCTAAAATC 
AATGAAATCA GAACTTTAAT TGATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLTF ( XM_006063127.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006063189.1 Bubalus bubalis helicase like transcription factor (HLTF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu19098
Clone ID Related Accession (Same CDS sequence)	XM_006063127.2 , XM_006063127.1
Accession Version	XM_006063127.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	helicase-like transcription factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037545.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006063127.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGGATCT TCTAAGGTTC AAGAAGATGC AGAATTTGCA 
TGTGCACTTA CTTCATCTAC CCCTGTAACA AAAAAGAAAA TGTTGAAAAG GGGGGCGTCT 
GCAGTGGAGA GTTCAAAGAA AACTGATGTT GAAGATAAAC CAAGAACAAC ACTGATCATC 
TGTCCACTTT CTGTATTAAG CAACTGGATG GACCAGTTTG GACAACACAT AAAATCAGAT 
GTGCACTTGA ATTTTTATGT TTATTATGGT CCTGATCGTG TTAGAGACCC AACATTGCTT 
TCAAAACAGG ATATTGTTTT GACGACATAC AATATTTTAA CTCATGACTA TGGTACTAAG 
GGTGATAGTC CATTACATAG CATAAGGTGG CTAAGAGTGA TCCTGGATGA AGGACATGCC 
ATACGGAATC CAAATGCTCA GCAAACAAAA GCTGTACTTG ATTTAGAAGC AGAAAGAAGA 
TGGGTTTTGA CAGGCACTCC AATTCAGAAT TCTTTAAAGG ACTTGTGGTC TCTTCTTTCC 
TTTTTAAAAC TTAAACCATT TGTTGATAGA GAATGGTGGC ATAGAACAAT ACAGCGTCCT 
GTCACAATGG GAGACGAAGC TGGACTTAGG CGTTTGCAGT CCCTAATTAA AAATATTACA 
CTTAGAAGAA CAAAGACAAG CAAAATTAAA GGAAAACCTG TTTTGGAGTT ACCAGAACGT 
AAAGTATTTA TTCAGCACAT TACACTTTCA GATGAAGAGA GAAAGATTTA CCAGTCTGTG 
AAAAATGAAG GCAAAGCTAC TATTGGAAGG TATTTTAATG AAGGGACTGT CCTTGCACAC 
TATGCAGATG TCCTGGGTCT TTTGCTTAGA TTGCGGCAGA TTTGTTGTCA TACACACCTT 
CTTGCAAATG CAGTGTCTTC CAGTGGTCCC TCAGGGAATG ATACACCTGA AGAACTACGA 
AAGAAGTTAA TAAAGAAGAT GAAGTTAATT CTAAGCTCAG GTTCTGATGA AGAATGTGCA 
ATTTGCTTGG ATTCTTTAAT GGCCCCTGTC ATAACACATT GTGCACATGT CTTCTGTAAA 
CCTTGTATTT GCCAAGTCAT TCAGAATGAG CAGCCTCATG CTAAATGCCC TTTATGCAGA 
AATGATATAC ATGGAGACAG TTTAATAGAA TGTCCTCCAG AAGAATTGGC ATGCAACACT 
GAGAAAAAGT CTAATATGGA ATGGACATCC AGTTCGAAGA TTAATGCTCT AATGCATGCA 
TTGATTGATT TAAGAAAGAA GAACCCCAAC ATAAAAAGTT TAGTTGTTTC TCAGTTTACA 
ACATTTCTGT CTTTAATAGA AACACCCCTC AGATCCTCTG GATTTGTATT TACTCGTTTG 
GATGGTTCCA TGGCCCAAAA AAAAAGAGTT GAATCAATTC AGTGTTTTCA AAACACTGAA 
GCAGGGTCTC CAACTATAAT GCTTCTATCC TTAAAAGCAG GTGGAGTTGG TTTGAATCTT 
TCTGCAGCTT CTCGAGTTTT TTTAATGGAT CCAGCCTGGA ATCCAGCTGC TGAAGATCAG 
TGCTTTGACA GATGCCATAG ACTTGGCCAG AAGCAAGAAG TTATTATCAC AAAATTCATT 
GTGAAGGATT CTGTTGAAGA AAACATGCTG AAAATACAAA ACACGAAGAG AGAACTTGCA 
GCTGGAGCCT TTGGAACTAA GAAAACAAAT GCGAGTGAAA TGAAACAAGC TAAAATCAAT 
GAAATCAGAA CTTTAATTGA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006063189.1
CDS	215..3241
Translation	MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDIIPPDDFLTSDEELDSVLFGTLRGHVVGLRYYTGVVNNNEMVALQREPTNPYDKNAIKVNNVNGNQVGHIKKDLAAALAYIMDNKLAQIEGVVPFGANNTFTMPLQMTFWGKEENRKAVLDELKKHGFKLGPAPKSLGFSLESGWGSGRAGPSYSMPVHTAVQMTTEQLKTEFDKLFEDLKEDDKTQEMEPAEAIETPLLPHQKQALAWMVSRENSEELPPFWEQRSDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGKSLPVERIKKNQLKKECNVYDESMELGGNNTSEKAAGLIKGSRCSGEPSISDVKGKNKHPKSEFSSSRSKRRKPAAVQYTESSDSEEVEPSELPQKMKGKLKRTQSETKIRVKGSSKVQEDAEFACALTSSTPVTKKKMLKRGASAVESSKKTDVEDKPRTTLIICPLSVLSNWMDQFGQHIKSDVHLNFYVYYGPDRVRDPTLLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFVDREWWHRTIQRPVTMGDEAGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGKATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTHLLANAVSSSGPSGNDTPEELRKKLIKKMKLILSSGSDEECAICLDSLMAPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHGDSLIECPPEELACNTEKKSNMEWTSSSKINALMHALIDLRKKNPNIKSLVVSQFTTFLSLIETPLRSSGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNTKRELAAGAFGTKKTNASEMKQAKINEIRTLIDL

Target ORF information:

RefSeq Version	XM_006063127.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis helicase like transcription factor (HLTF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006063127.2

ORF Insert Sequence:

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGGATCT TCTAAGGTTC AAGAAGATGC AGAATTTGCA 
TGTGCACTTA CTTCATCTAC CCCTGTAACA AAAAAGAAAA TGTTGAAAAG GGGGGCGTCT 
GCAGTGGAGA GTTCAAAGAA AACTGATGTT GAAGATAAAC CAAGAACAAC ACTGATCATC 
TGTCCACTTT CTGTATTAAG CAACTGGATG GACCAGTTTG GACAACACAT AAAATCAGAT 
GTGCACTTGA ATTTTTATGT TTATTATGGT CCTGATCGTG TTAGAGACCC AACATTGCTT 
TCAAAACAGG ATATTGTTTT GACGACATAC AATATTTTAA CTCATGACTA TGGTACTAAG 
GGTGATAGTC CATTACATAG CATAAGGTGG CTAAGAGTGA TCCTGGATGA AGGACATGCC 
ATACGGAATC CAAATGCTCA GCAAACAAAA GCTGTACTTG ATTTAGAAGC AGAAAGAAGA 
TGGGTTTTGA CAGGCACTCC AATTCAGAAT TCTTTAAAGG ACTTGTGGTC TCTTCTTTCC 
TTTTTAAAAC TTAAACCATT TGTTGATAGA GAATGGTGGC ATAGAACAAT ACAGCGTCCT 
GTCACAATGG GAGACGAAGC TGGACTTAGG CGTTTGCAGT CCCTAATTAA AAATATTACA 
CTTAGAAGAA CAAAGACAAG CAAAATTAAA GGAAAACCTG TTTTGGAGTT ACCAGAACGT 
AAAGTATTTA TTCAGCACAT TACACTTTCA GATGAAGAGA GAAAGATTTA CCAGTCTGTG 
AAAAATGAAG GCAAAGCTAC TATTGGAAGG TATTTTAATG AAGGGACTGT CCTTGCACAC 
TATGCAGATG TCCTGGGTCT TTTGCTTAGA TTGCGGCAGA TTTGTTGTCA TACACACCTT 
CTTGCAAATG CAGTGTCTTC CAGTGGTCCC TCAGGGAATG ATACACCTGA AGAACTACGA 
AAGAAGTTAA TAAAGAAGAT GAAGTTAATT CTAAGCTCAG GTTCTGATGA AGAATGTGCA 
ATTTGCTTGG ATTCTTTAAT GGCCCCTGTC ATAACACATT GTGCACATGT CTTCTGTAAA 
CCTTGTATTT GCCAAGTCAT TCAGAATGAG CAGCCTCATG CTAAATGCCC TTTATGCAGA 
AATGATATAC ATGGAGACAG TTTAATAGAA TGTCCTCCAG AAGAATTGGC ATGCAACACT 
GAGAAAAAGT CTAATATGGA ATGGACATCC AGTTCGAAGA TTAATGCTCT AATGCATGCA 
TTGATTGATT TAAGAAAGAA GAACCCCAAC ATAAAAAGTT TAGTTGTTTC TCAGTTTACA 
ACATTTCTGT CTTTAATAGA AACACCCCTC AGATCCTCTG GATTTGTATT TACTCGTTTG 
GATGGTTCCA TGGCCCAAAA AAAAAGAGTT GAATCAATTC AGTGTTTTCA AAACACTGAA 
GCAGGGTCTC CAACTATAAT GCTTCTATCC TTAAAAGCAG GTGGAGTTGG TTTGAATCTT 
TCTGCAGCTT CTCGAGTTTT TTTAATGGAT CCAGCCTGGA ATCCAGCTGC TGAAGATCAG 
TGCTTTGACA GATGCCATAG ACTTGGCCAG AAGCAAGAAG TTATTATCAC AAAATTCATT 
GTGAAGGATT CTGTTGAAGA AAACATGCTG AAAATACAAA ACACGAAGAG AGAACTTGCA 
GCTGGAGCCT TTGGAACTAA GAAAACAAAT GCGAGTGAAA TGAAACAAGC TAAAATCAAT 
GAAATCAGAA CTTTAATTGA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLTF ( XM_006063127.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006063189.1 Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu19098
Clone ID Related Accession (Same CDS sequence)	XM_006063127.2 , XM_006063127.1
Accession Version	XM_006063127.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	helicase-like transcription factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGGATCT TCTAAGGTTC AAGAAGATGC AGAATTTGCA 
TGTGCACTTA CTTCATCTAC CCCTGTAACA AAAAAGAAAA TGTTGAAAAG GGGGGCGTCT 
GCAGTGGAGA GTTCAAAGAA AACTGATGTT GAAGATAAAC CAAGAACAAC ACTGATCATC 
TGTCCACTTT CTGTATTAAG CAACTGGATG GACCAGTTTG GACAACACAT AAAATCAGAT 
GTGCACTTGA ATTTTTATGT TTATTATGGT CCTGATCGTG TTAGAGACCC AACATTGCTT 
TCAAAACAGG ATATTGTTTT GACGACATAC AATATTTTAA CTCATGACTA TGGTACTAAG 
GGTGATAGTC CATTACATAG CATAAGGTGG CTAAGAGTGA TCCTGGATGA AGGACATGCC 
ATACGGAATC CAAATGCTCA GCAAACAAAA GCTGTACTTG ATTTAGAAGC AGAAAGAAGA 
TGGGTTTTGA CAGGCACTCC AATTCAGAAT TCTTTAAAGG ACTTGTGGTC TCTTCTTTCC 
TTTTTAAAAC TTAAACCATT TGTTGATAGA GAATGGTGGC ATAGAACAAT ACAGCGTCCT 
GTCACAATGG GAGACGAAGC TGGACTTAGG CGTTTGCAGT CCCTAATTAA AAATATTACA 
CTTAGAAGAA CAAAGACAAG CAAAATTAAA GGAAAACCTG TTTTGGAGTT ACCAGAACGT 
AAAGTATTTA TTCAGCACAT TACACTTTCA GATGAAGAGA GAAAGATTTA CCAGTCTGTG 
AAAAATGAAG GCAAAGCTAC TATTGGAAGG TATTTTAATG AAGGGACTGT CCTTGCACAC 
TATGCAGATG TCCTGGGTCT TTTGCTTAGA TTGCGGCAGA TTTGTTGTCA TACACACCTT 
CTTGCAAATG CAGTGTCTTC CAGTGGTCCC TCAGGGAATG ATACACCTGA AGAACTACGA 
AAGAAGTTAA TAAAGAAGAT GAAGTTAATT CTAAGCTCAG GTTCTGATGA AGAATGTGCA 
ATTTGCTTGG ATTCTTTAAT GGCCCCTGTC ATAACACATT GTGCACATGT CTTCTGTAAA 
CCTTGTATTT GCCAAGTCAT TCAGAATGAG CAGCCTCATG CTAAATGCCC TTTATGCAGA 
AATGATATAC ATGGAGACAG TTTAATAGAA TGTCCTCCAG AAGAATTGGC ATGCAACACT 
GAGAAAAAGT CTAATATGGA ATGGACATCC AGTTCGAAGA TTAATGCTCT AATGCATGCA 
TTGATTGATT TAAGAAAGAA GAACCCCAAC ATAAAAAGTT TAGTTGTTTC TCAGTTTACA 
ACATTTCTGT CTTTAATAGA AACACCCCTC AGATCCTCTG GATTTGTATT TACTCGTTTG 
GATGGTTCCA TGGCCCAAAA AAAAAGAGTT GAATCAATTC AGTGTTTTCA AAACACTGAA 
GCAGGGTCTC CAACTATAAT GCTTCTATCC TTAAAAGCAG GTGGAGTTGG TTTGAATCTT 
TCTGCAGCTT CTCGAGTTTT TTTAATGGAT CCAGCCTGGA ATCCAGCTGC TGAAGATCAG 
TGCTTTGACA GATGCCATAG ACTTGGCCAG AAGCAAGAAG TTATTATCAC AAAATTCATT 
GTGAAGGATT CTGTTGAAGA AAACATGCTG AAAATACAAA ACACGAAGAG AGAACTTGCA 
GCTGGAGCCT TTGGAACTAA GAAAACAAAT GCGAGTGAAA TGAAACAAGC TAAAATCAAT 
GAAATCAGAA CTTTAATTGA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006063189.1
CDS	203..3229
Translation	MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDIIPPDDFLTSDEELDSVLFGTLRGHVVGLRYYTGVVNNNEMVALQREPTNPYDKNAIKVNNVNGNQVGHIKKDLAAALAYIMDNKLAQIEGVVPFGANNTFTMPLQMTFWGKEENRKAVLDELKKHGFKLGPAPKSLGFSLESGWGSGRAGPSYSMPVHTAVQMTTEQLKTEFDKLFEDLKEDDKTQEMEPAEAIETPLLPHQKQALAWMVSRENSEELPPFWEQRSDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGKSLPVERIKKNQLKKECNVYDESMELGGNNTSEKAAGLIKGSRCSGEPSISDVKGKNKHPKSEFSSSRSKRRKPAAVQYTESSDSEEVEPSELPQKMKGKLKRTQSETKIRVKGSSKVQEDAEFACALTSSTPVTKKKMLKRGASAVESSKKTDVEDKPRTTLIICPLSVLSNWMDQFGQHIKSDVHLNFYVYYGPDRVRDPTLLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFVDREWWHRTIQRPVTMGDEAGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGKATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTHLLANAVSSSGPSGNDTPEELRKKLIKKMKLILSSGSDEECAICLDSLMAPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHGDSLIECPPEELACNTEKKSNMEWTSSSKINALMHALIDLRKKNPNIKSLVVSQFTTFLSLIETPLRSSGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNTKRELAAGAFGTKKTNASEMKQAKINEIRTLIDL

Target ORF information:

RefSeq Version	XM_006063127.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006063127.1

ORF Insert Sequence:

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGGATC TAGATGTAGT 
GGAGAACCCA GTATTTCAGA TGTCAAGGGA AAGAATAAAC ATCCCAAGTC AGAATTCTCT 
AGCTCCCGCT CCAAAAGGAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGGATCT TCTAAGGTTC AAGAAGATGC AGAATTTGCA 
TGTGCACTTA CTTCATCTAC CCCTGTAACA AAAAAGAAAA TGTTGAAAAG GGGGGCGTCT 
GCAGTGGAGA GTTCAAAGAA AACTGATGTT GAAGATAAAC CAAGAACAAC ACTGATCATC 
TGTCCACTTT CTGTATTAAG CAACTGGATG GACCAGTTTG GACAACACAT AAAATCAGAT 
GTGCACTTGA ATTTTTATGT TTATTATGGT CCTGATCGTG TTAGAGACCC AACATTGCTT 
TCAAAACAGG ATATTGTTTT GACGACATAC AATATTTTAA CTCATGACTA TGGTACTAAG 
GGTGATAGTC CATTACATAG CATAAGGTGG CTAAGAGTGA TCCTGGATGA AGGACATGCC 
ATACGGAATC CAAATGCTCA GCAAACAAAA GCTGTACTTG ATTTAGAAGC AGAAAGAAGA 
TGGGTTTTGA CAGGCACTCC AATTCAGAAT TCTTTAAAGG ACTTGTGGTC TCTTCTTTCC 
TTTTTAAAAC TTAAACCATT TGTTGATAGA GAATGGTGGC ATAGAACAAT ACAGCGTCCT 
GTCACAATGG GAGACGAAGC TGGACTTAGG CGTTTGCAGT CCCTAATTAA AAATATTACA 
CTTAGAAGAA CAAAGACAAG CAAAATTAAA GGAAAACCTG TTTTGGAGTT ACCAGAACGT 
AAAGTATTTA TTCAGCACAT TACACTTTCA GATGAAGAGA GAAAGATTTA CCAGTCTGTG 
AAAAATGAAG GCAAAGCTAC TATTGGAAGG TATTTTAATG AAGGGACTGT CCTTGCACAC 
TATGCAGATG TCCTGGGTCT TTTGCTTAGA TTGCGGCAGA TTTGTTGTCA TACACACCTT 
CTTGCAAATG CAGTGTCTTC CAGTGGTCCC TCAGGGAATG ATACACCTGA AGAACTACGA 
AAGAAGTTAA TAAAGAAGAT GAAGTTAATT CTAAGCTCAG GTTCTGATGA AGAATGTGCA 
ATTTGCTTGG ATTCTTTAAT GGCCCCTGTC ATAACACATT GTGCACATGT CTTCTGTAAA 
CCTTGTATTT GCCAAGTCAT TCAGAATGAG CAGCCTCATG CTAAATGCCC TTTATGCAGA 
AATGATATAC ATGGAGACAG TTTAATAGAA TGTCCTCCAG AAGAATTGGC ATGCAACACT 
GAGAAAAAGT CTAATATGGA ATGGACATCC AGTTCGAAGA TTAATGCTCT AATGCATGCA 
TTGATTGATT TAAGAAAGAA GAACCCCAAC ATAAAAAGTT TAGTTGTTTC TCAGTTTACA 
ACATTTCTGT CTTTAATAGA AACACCCCTC AGATCCTCTG GATTTGTATT TACTCGTTTG 
GATGGTTCCA TGGCCCAAAA AAAAAGAGTT GAATCAATTC AGTGTTTTCA AAACACTGAA 
GCAGGGTCTC CAACTATAAT GCTTCTATCC TTAAAAGCAG GTGGAGTTGG TTTGAATCTT 
TCTGCAGCTT CTCGAGTTTT TTTAATGGAT CCAGCCTGGA ATCCAGCTGC TGAAGATCAG 
TGCTTTGACA GATGCCATAG ACTTGGCCAG AAGCAAGAAG TTATTATCAC AAAATTCATT 
GTGAAGGATT CTGTTGAAGA AAACATGCTG AAAATACAAA ACACGAAGAG AGAACTTGCA 
GCTGGAGCCT TTGGAACTAA GAAAACAAAT GCGAGTGAAA TGAAACAAGC TAAAATCAAT 
GAAATCAGAA CTTTAATTGA TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HLTF ( XM_006063128.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006063190.1 Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X3, mRNA.

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CloneID	OBu19099
Clone ID Related Accession (Same CDS sequence)	XM_006063128.1
Accession Version	XM_006063128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	helicase-like transcription factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785077.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGACAG ATCTAGATGT 
AGTGGAGAAC CCAGTATTTC AGATGTCAAG GGAAAGAATA AACATCCCAA GTCAGAATTC 
TCTAGCTCCC GCTCCAAAAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGCAGGA TCTTCTAAGG TTCAAGAAGA TGCAGAATTT 
GCATGTGCAC TTACTTCATC TACCCCTGTA ACAAAAAAGA AAATGTTGAA AAGGGGGGCG 
TCTGCAGTGG AGAGTTCAAA GAAAACTGAT GTTGAAGATA AACCAAGAAC AACACTGATC 
ATCTGTCCAC TTTCTGTATT AAGCAACTGG ATGGACCAGT TTGGACAACA CATAAAATCA 
GATGTGCACT TGAATTTTTA TGTTTATTAT GGTCCTGATC GTGTTAGAGA CCCAACATTG 
CTTTCAAAAC AGGATATTGT TTTGACGACA TACAATATTT TAACTCATGA CTATGGTACT 
AAGGGTGATA GTCCATTACA TAGCATAAGG TGGCTAAGAG TGATCCTGGA TGAAGGACAT 
GCCATACGGA ATCCAAATGC TCAGCAAACA AAAGCTGTAC TTGATTTAGA AGCAGAAAGA 
AGATGGGTTT TGACAGGCAC TCCAATTCAG AATTCTTTAA AGGACTTGTG GTCTCTTCTT 
TCCTTTTTAA AACTTAAACC ATTTGTTGAT AGAGAATGGT GGCATAGAAC AATACAGCGT 
CCTGTCACAA TGGGAGACGA AGCTGGACTT AGGCGTTTGC AGTCCCTAAT TAAAAATATT 
ACACTTAGAA GAACAAAGAC AAGCAAAATT AAAGGAAAAC CTGTTTTGGA GTTACCAGAA 
CGTAAAGTAT TTATTCAGCA CATTACACTT TCAGATGAAG AGAGAAAGAT TTACCAGTCT 
GTGAAAAATG AAGGCAAAGC TACTATTGGA AGGTATTTTA ATGAAGGGAC TGTCCTTGCA 
CACTATGCAG ATGTCCTGGG TCTTTTGCTT AGATTGCGGC AGATTTGTTG TCATACACAC 
CTTCTTGCAA ATGCAGTGTC TTCCAGTGGT CCCTCAGGGA ATGATACACC TGAAGAACTA 
CGAAAGAAGT TAATAAAGAA GATGAAGTTA ATTCTAAGCT CAGGTTCTGA TGAAGAATGT 
GCAATTTGCT TGGATTCTTT AATGGCCCCT GTCATAACAC ATTGTGCACA TGTCTTCTGT 
AAACCTTGTA TTTGCCAAGT CATTCAGAAT GAGCAGCCTC ATGCTAAATG CCCTTTATGC 
AGAAATGATA TACATGGAGA CAGTTTAATA GAATGTCCTC CAGAAGAATT GGCATGCAAC 
ACTGAGAAAA AGTCTAATAT GGAATGGACA TCCAGTTCGA AGATTAATGC TCTAATGCAT 
GCATTGATTG ATTTAAGAAA GAAGAACCCC AACATAAAAA GTTTAGTTGT TTCTCAGTTT 
ACAACATTTC TGTCTTTAAT AGAAACACCC CTCAGATCCT CTGGATTTGT ATTTACTCGT 
TTGGATGGTT CCATGGCCCA AAAAAAAAGA GTTGAATCAA TTCAGTGTTT TCAAAACACT 
GAAGCAGGGT CTCCAACTAT AATGCTTCTA TCCTTAAAAG CAGGTGGAGT TGGTTTGAAT 
CTTTCTGCAG CTTCTCGAGT TTTTTTAATG GATCCAGCCT GGAATCCAGC TGCTGAAGAT 
CAGTGCTTTG ACAGATGCCA TAGACTTGGC CAGAAGCAAG AAGTTATTAT CACAAAATTC 
ATTGTGAAGG ATTCTGTTGA AGAAAACATG CTGAAAATAC AAAACACGAA GAGAGAACTT 
GCAGCTGGAG CCTTTGGAAC TAAGAAAACA AATGCGAGTG AAATGAAACA AGCTAAAATC 
AATGAAATCA GAACTTTAAT TGATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006063190.1
CDS	203..3232
Translation	MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDIIPPDDFLTSDEELDSVLFGTLRGHVVGLRYYTGVVNNNEMVALQREPTNPYDKNAIKVNNVNGNQVGHIKKDLAAALAYIMDNKLAQIEGVVPFGANNTFTMPLQMTFWGKEENRKAVLDELKKHGFKLGPAPKSLGFSLESGWGSGRAGPSYSMPVHTAVQMTTEQLKTEFDKLFEDLKEDDKTQEMEPAEAIETPLLPHQKQALAWMVSRENSEELPPFWEQRSDLYYNTITNFSEKDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGKSLPVERIKKNQLKKECNVYDESMELGGNNTSEKAAGLIKDRSRCSGEPSISDVKGKNKHPKSEFSSSRSKRKPAAVQYTESSDSEEVEPSELPQKMKGKLKRTQSETKIRVKAGSSKVQEDAEFACALTSSTPVTKKKMLKRGASAVESSKKTDVEDKPRTTLIICPLSVLSNWMDQFGQHIKSDVHLNFYVYYGPDRVRDPTLLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHAIRNPNAQQTKAVLDLEAERRWVLTGTPIQNSLKDLWSLLSFLKLKPFVDREWWHRTIQRPVTMGDEAGLRRLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGKATIGRYFNEGTVLAHYADVLGLLLRLRQICCHTHLLANAVSSSGPSGNDTPEELRKKLIKKMKLILSSGSDEECAICLDSLMAPVITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHGDSLIECPPEELACNTEKKSNMEWTSSSKINALMHALIDLRKKNPNIKSLVVSQFTTFLSLIETPLRSSGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLSLKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNTKRELAAGAFGTKKTNASEMKQAKINEIRTLIDL

Target ORF information:

RefSeq Version	XM_006063128.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis helicase-like transcription factor (HLTF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006063128.1

ORF Insert Sequence:

ATGTCCTGGA TGTTCAAGAG AGATCCTGTT TGGAAGTACT TGCAGACTGT CCAGTATGGA 
GTCCATGGAA ATTTTCCACG CCTCTCATAT CCAACTTTCT TTCCTCGTTT TGAATTTCAA 
GATATTATTC CTCCAGATGA CTTCCTAACA AGTGATGAAG AGTTAGATTC AGTTTTATTT 
GGAACTTTGA GAGGCCATGT CGTTGGACTA CGCTATTACA CAGGAGTAGT TAATAATAAT 
GAAATGGTTG CATTACAACG AGAGCCGACT AACCCGTATG ATAAGAATGC AATTAAAGTA 
AACAATGTGA ATGGAAATCA GGTTGGTCAT ATAAAGAAAG ATCTTGCAGC TGCCTTGGCC 
TATATCATGG ACAACAAATT GGCACAAATT GAAGGGGTAG TTCCTTTTGG TGCAAACAAT 
ACTTTTACCA TGCCTCTGCA AATGACATTT TGGGGAAAAG AAGAAAATAG AAAAGCAGTT 
TTAGATGAGT TAAAGAAACA TGGATTTAAA TTGGGACCTG CACCAAAATC TTTAGGGTTC 
AGTTTGGAAA GTGGTTGGGG CTCTGGAAGA GCTGGACCAA GCTACAGTAT GCCAGTTCAT 
ACTGCAGTAC AGATGACAAC TGAACAGCTT AAGACTGAGT TTGACAAATT GTTTGAAGAT 
TTAAAAGAAG ATGATAAAAC TCAGGAAATG GAACCAGCTG AGGCTATTGA AACACCATTG 
CTTCCACATC AAAAACAAGC TCTAGCTTGG ATGGTTTCAC GGGAAAACAG TGAAGAACTT 
CCGCCATTCT GGGAACAGCG AAGTGACTTA TACTATAACA CAATAACAAA TTTTTCTGAA 
AAGGACCGAC CAGAAAATGT TCATGGAGGA ATTTTAGCTG ATGATATGGG TTTGGGCAAA 
ACTCTGACAG CCATTGCAGT GATTCTCACC AACTTCCATG ATGGCAAGTC TCTTCCTGTT 
GAAAGAATTA AGAAAAATCA ACTGAAGAAG GAATGTAATG TTTACGATGA ATCTATGGAA 
CTTGGAGGAA ACAATACCAG TGAAAAGGCA GCTGGACTAA TCAAAGACAG ATCTAGATGT 
AGTGGAGAAC CCAGTATTTC AGATGTCAAG GGAAAGAATA AACATCCCAA GTCAGAATTC 
TCTAGCTCCC GCTCCAAAAG AAAACCTGCT GCTGTACAGT ACACTGAAAG TAGTGATTCA 
GAGGAAGTTG AACCAAGTGA ATTGCCACAG AAAATGAAAG GCAAACTGAA AAGAACACAA 
TCAGAGACTA AAATCAGAGT AAAAGCAGGA TCTTCTAAGG TTCAAGAAGA TGCAGAATTT 
GCATGTGCAC TTACTTCATC TACCCCTGTA ACAAAAAAGA AAATGTTGAA AAGGGGGGCG 
TCTGCAGTGG AGAGTTCAAA GAAAACTGAT GTTGAAGATA AACCAAGAAC AACACTGATC 
ATCTGTCCAC TTTCTGTATT AAGCAACTGG ATGGACCAGT TTGGACAACA CATAAAATCA 
GATGTGCACT TGAATTTTTA TGTTTATTAT GGTCCTGATC GTGTTAGAGA CCCAACATTG 
CTTTCAAAAC AGGATATTGT TTTGACGACA TACAATATTT TAACTCATGA CTATGGTACT 
AAGGGTGATA GTCCATTACA TAGCATAAGG TGGCTAAGAG TGATCCTGGA TGAAGGACAT 
GCCATACGGA ATCCAAATGC TCAGCAAACA AAAGCTGTAC TTGATTTAGA AGCAGAAAGA 
AGATGGGTTT TGACAGGCAC TCCAATTCAG AATTCTTTAA AGGACTTGTG GTCTCTTCTT 
TCCTTTTTAA AACTTAAACC ATTTGTTGAT AGAGAATGGT GGCATAGAAC AATACAGCGT 
CCTGTCACAA TGGGAGACGA AGCTGGACTT AGGCGTTTGC AGTCCCTAAT TAAAAATATT 
ACACTTAGAA GAACAAAGAC AAGCAAAATT AAAGGAAAAC CTGTTTTGGA GTTACCAGAA 
CGTAAAGTAT TTATTCAGCA CATTACACTT TCAGATGAAG AGAGAAAGAT TTACCAGTCT 
GTGAAAAATG AAGGCAAAGC TACTATTGGA AGGTATTTTA ATGAAGGGAC TGTCCTTGCA 
CACTATGCAG ATGTCCTGGG TCTTTTGCTT AGATTGCGGC AGATTTGTTG TCATACACAC 
CTTCTTGCAA ATGCAGTGTC TTCCAGTGGT CCCTCAGGGA ATGATACACC TGAAGAACTA 
CGAAAGAAGT TAATAAAGAA GATGAAGTTA ATTCTAAGCT CAGGTTCTGA TGAAGAATGT 
GCAATTTGCT TGGATTCTTT AATGGCCCCT GTCATAACAC ATTGTGCACA TGTCTTCTGT 
AAACCTTGTA TTTGCCAAGT CATTCAGAAT GAGCAGCCTC ATGCTAAATG CCCTTTATGC 
AGAAATGATA TACATGGAGA CAGTTTAATA GAATGTCCTC CAGAAGAATT GGCATGCAAC 
ACTGAGAAAA AGTCTAATAT GGAATGGACA TCCAGTTCGA AGATTAATGC TCTAATGCAT 
GCATTGATTG ATTTAAGAAA GAAGAACCCC AACATAAAAA GTTTAGTTGT TTCTCAGTTT 
ACAACATTTC TGTCTTTAAT AGAAACACCC CTCAGATCCT CTGGATTTGT ATTTACTCGT 
TTGGATGGTT CCATGGCCCA AAAAAAAAGA GTTGAATCAA TTCAGTGTTT TCAAAACACT 
GAAGCAGGGT CTCCAACTAT AATGCTTCTA TCCTTAAAAG CAGGTGGAGT TGGTTTGAAT 
CTTTCTGCAG CTTCTCGAGT TTTTTTAATG GATCCAGCCT GGAATCCAGC TGCTGAAGAT 
CAGTGCTTTG ACAGATGCCA TAGACTTGGC CAGAAGCAAG AAGTTATTAT CACAAAATTC 
ATTGTGAAGG ATTCTGTTGA AGAAAACATG CTGAAAATAC AAAACACGAA GAGAGAACTT 
GCAGCTGGAG CCTTTGGAAC TAAGAAAACA AATGCGAGTG AAATGAAACA AGCTAAAATC 
AATGAAATCA GAACTTTAAT TGATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HLTF cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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