NUP188 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following NUP188 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUP188 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu20883 XM_006065325.1
Latest version!
Bubalus bubalis nucleoporin 188kDa (NUP188), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBu46690 XM_025261803.1
Latest version!
Bubalus bubalis nucleoporin 188 (NUP188), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu46691 XM_025261804.1
Latest version!
Bubalus bubalis nucleoporin 188 (NUP188), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu46692 XM_025261805.1
Latest version!
Bubalus bubalis nucleoporin 188 (NUP188), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBu20882 XM_006065324.1
Latest version!
Bubalus bubalis nucleoporin 188kDa (NUP188), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu20883
    Clone ID Related Accession (Same CDS sequence) XM_006065325.1
    Accession Version XM_006065325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nucleoporin NUP188 homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785166.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCCA GCCCTGCCTG GAGCAGTAGA GAACTGTGGA CAATTCTGCT AGGAAGGTCA 
    GCTCTTAGAG AACTGAATCA GATTGAGGCA GAGCTGAATA AACATTGGCA GCGGCTGTTA
    GAAGGACTTT CTTACTATAA ACCTCCCAGT CCAAGTTCAG CTGAAAAAGT GAAAGCTAGT
    AAAGATGTAG CTTCACCACT GAAGGAACTG GGTTTAAGAA TCAGCAAGTT TTTGGGTCTT
    GATGAAGAAC AGAGTGTGCA GTTACTCCAG TGTTACCTTC AAGAGGATTA CAGAGGTACT
    CGGGACTCAC TAAAGACGGT GCTGCAAGAT GAGAGGCAGA GCCAGGCCTT AATCCTGAAG
    ATTGCAGATT ATTACTATGA AGAAAGAACT TGTATTCTTC GTTGCGTCTT ACACCTTCTC
    ACTTATTTCC AAGATGAAAG ACACCCCTAT AGGGTTGAAT ATGCCGACTG TGTGGATAAA
    TTGGAAAAGG AGCTAGTTAC AAAATATAGA CAGCAGTTTG AAGAGCTTTA TAAAACTGAA
    GCACCAACGT GGGAGACACA TGGAAACCTC ATGACTGAGC GCCAGGTGTC TCGCTGGTTT
    GTTCAGTGCC TTCGGGAGCA GTCCATGCTG CTAGAGATTA TTTTCCTGTA TTATGCATAC
    TTTGAGATGG CACCTAGTGA CCTATTGGTG TTAACCAAGA TGTTTAAAGA GCAAGGATTT
    GGTAGTAGGC AGACTAACAG GCACCTGGTA GATGAGACCA TGGATCCTTT TGTGGATCGG
    ATTGGCTACT TCAGTGCCCT TATTCTGGTG GAAGGCATGG ACATTGAATC CTTGCATAAG
    TGTGCTTTGG ATGACAGAAG AGAACTGCAC CAGTTTGCCC AGGATGGACT TATTTGTCAG
    GATATGGACC GTTTGATGTT GACCTTTGGG GACATTCCGC ATCATGCCCC AGTGCTTTTG
    GCATGGGCCC TCCTCCGTCA CACCCTGAAT CCAGAAGAGA CGAGCAGTGT GGTCCGGAAG
    ATAGGGGGGA CAGCCATCCA ACTGAACGTC TTTCAGTACT TGACCCGACT GCTCCGCTCG
    CTGGCCAGTG GGGGAAATGA CTGCACCACC AGCACTGCCT GCATGTGTGT CTACGGACTC
    CTTTCTTTTG TCCTGACCTC CCTGGAGCTG CACACCCTGG GCAATCAGCA GGATGTAATT
    GACACAGCAT GTGAAGTCCT GGCAGACCCT TCTCTCCCAG AACTCTTCTG GGGAACAGAA
    CCAACCTCTG GTCTCGGGAT CATTCTGGAC AGTGTATGTG GAATGTTTCC CCATCTCCTC
    TCTCCACTCC TGCAGTTGCT TCGAGCCCTT GTATCAGGGA AGTCTACTGC TAAGAAGGTG
    TATAGTTTCT TGGATAAGAT GTCTTTCTAC AATGAACTCT ATAAGCACAA GCCCCATGAT
    GTGATCTCCC ATGAAGATGG AACCCTTTGG CGGAGGCAAA CACCCAAACT CCTTTACCCT
    CTAGGGGGTC AGACCAACCT TCGCATACCT CAAGGCACCG TGGGCCAAGT GATGCTGGAC
    GATAGGGCCT ACCTGGTGCG CTGGGAATAC TCGTACAGCA GCTGGACCCT CTTCACCTGC
    GAGATTGAGA TGCTGCTGCA TGTCGTTTCC ACTGCAGATG TGATTCAGCA CTGCCAGCGG
    GTCAAACCCA TCATCGACCT GGTCCATAAG GTCATCAGCA CAGACCTGTC GATAGCAGAC
    TGTCTCCTGC CCATCACGTC CCGCATCTAC ATGCTACTGC AGCGGTTAAC AACAGTCATC
    TCCCCGCCTG TGGATGTCAT TGCTTCTTGC GTCAATTGCT TGACTGTTTT GGCTGCCCGG
    AACCCAGCAA AGGTCTGGAC TGATCTTCTT CACACAGGCT TCTTGCCTTT CGTGGCCCAC
    CAGGTCTCCA GCATGAGTCA GATGATCAGT GCTGAGGGGA TGAATGCTGG TGGGTATGGA
    AACCTCTTGA TGAATAGTGA ACAGCCCCAG GGCGAGTATG GGGTCACTGT TGCCTTTCTG
    CGCTTGATCA CCACTCTTGT GAAGGGGCAG CTTGGTAGTA CCCAGAGCCA AGGGCTGGTG
    CCTTGTGTCA TGTTTGTGCT GAAGGAGATG CTGCCAAGTT ACCACAAGTG GCGCTACAAC
    TCCCATGGTG TGAGAGAACA GATTGGTTGC CTGATCCTGG AGCTGATTCA TTCAATACTG
    AACCTGTGCC ATGAGACAGA CCTGCATAGC AGTCACACCC CCAGCCTGCA GTCTCTCTGC
    ATCTGCAGCC TGGCCTACAC AGAAGCAGGA CAGACAGTAA TCAATATCAT GGGCATCGGT
    GTGGACACCA TCGACATGGT CATGGCTGCT CAGCCCCGCA GCGATGGGGC AGAGGGCCAG
    GGCCAGGGCC AGCTGCTGAT CAAGACAGTG AAATTGGCGT TCTCCGTCAC CAACAATGTC
    ATTCGGCTGA AACCGCCTTC TAATGTGGTG TCCCCTCTGG AACAGGCTCT CACACAGCAT
    GGTGCCCATG GGAACAACCT TATTGCTGTT CTTGCCAAAT ACATCTACCA CAAACACGAC
    CCAGCTCTGC CTCGTCTTGC CATCCAGCTG CTGAAACGTC TGGCCACGGT GGCCCCCATG
    TCAGTGTATG CCTGCCTGGG CAGTGATGCG GCTGCCATCC GAGACGCCTT CCTGACCCGA
    CTGCAGAGCA AGATTGAGGA TATGCGCATC AAAGTCATGA TTCTGGAATT CCTCACGGTT
    GCTGTAGAGA CGCAGCCAGG CCTCATTGAG CTGTTTCTGA ATCTGGAGGT GAAGGATGGC
    AGCGATGGCT CCAAGGAATT CAGCCTGGGG GTGTGGAGCT GTCTCCACGC GGTGCTGGAG
    CTGATCGGTT CCCAGCAGCA GGACCGCTAC TGGTGCCCCC CACTGCTGCA CCGGGCAGCC
    ATCGCCTTTT TGCACGCCCT GTGGCAGGAC CGGCGGGACA GTGCCATGCT GGTCCTCAGA
    ACCAAACCCA AGTTTTGGGA GAATCTAACG AGTCCGCTAT TTGGTACTCT TTCTCCTCCC
    TCGGAAACAT CTGAGCCCAG CATCCTGGAA ACCTGTGCCC TAATCATGAA GATAATTTGC
    TTGGAGATAT ACTATGTAGT TAAGGGCTCA TTAGACCAAT CATTAAAAGA TACACTCAAG
    AAATTTTCCA GCGAGAAGCG CTTTGCCTAC TGGTCGGGGT ATGTCAAGTC CTTGGCGGTT
    CACATGGCCG ACACAGAGGG CAGCAGCTGC ACCTCCCTGG TAGAGTATCA GATGCTGGTC
    TCTGCCTGGA GGATGTTTCT CATCATTGCC ACCAGTCACG CGGAGATAAC GCACCTGACA
    GACTCTGCAG TGCGTCGCCA GCTCTTTCTT GACGTGCTCG AGGGGACCAA GGCGTTACTC
    CTCGTCCCTA CCTCCGTGAA CTGCCTCCGC CTCGGCTCCA TGACGTGCAC CCTGCTGCTT
    ATTCTCCTCC GGCAGTGGAA AAGAGAGTTA GGGTCTGTGG ATGAAATCCT CGGGCCCCTG
    ACGGAGATCC TGGAGGGAGT GCTGCAGGCA GACCAGCAGC TTATGGAGAA GACCAAGGCC
    AAGGTGTTCT CAGCGTTCAT CACGGTGCTG CAGATGAAGG AGATGAGCGT GAATGACATC
    CCCCAGTACT CCCAGCTGGT GCTGAACGTC TGTGAGACCC TTCAGGAAGA GGTGATCGCA
    CTTTTCGACC ACACCCGCCA CAGCCTGGCG GCGGGCAGTG CCACCGAGGA CAAGGACAGC
    ATGGAGACCG ACGACTGCTC CCGGCTCCGG CACAAGGACC AGCGGGACGG GGTCTGTGTC
    CTGGGCCTGC ACCTGGCCAA GGAGCTGTGT GAGGTGGATG AGGACGGGGA CTCGTGGCTG
    CAGGTGACCC GCCGGCTCCC CATCCTGCCG ACCCTCTTCA CCACCTTGGA GGTGAGCCTG
    CGCGTGAAGC AGAACCTGCA CTTCACCGAG GCGGCGCTGC ACCTGCTCCT CACGCTGGCC
    CGCACCCAGC AGGGGGCCAC AGCCGTGGCC GGGGCCGGCG TCACCCAGAG CATTTGCTTG
    CCTCTCCTGA GTGTGTACCA GCTCAGCGCC AACGGGACAG TGCAGACACC CGGCACCTCT
    CGGAAGTCCC TGGACGCCCC CTCTTGGCCT GGCGTCTACC GTCTCTCCAT GTCCTTGATG
    GAGCGGCTCC TCAAAACCCT GCGCTACAAC TTCCTGACCG AGGCCCTGGA CTTCGTGGGG
    GTTCACCAGG AGCGGACCTT ACAGTGCCTC AATGCCGTGA GGACGGTGCA GAGTCTGGCG
    TGCCTGGAGG AGGCTGACCA CACCGTGGGC TTCCTCCTAC AGCTCTCCAG CTTCAGGAAG
    GAGTGGCACT TCCACCTGCC TCAGCTCATG CGGGATGTCC AGGTGAACCT CGGCTACCTG
    TGCCAGGCCT GCACCTCCCT CCTGCACAGC CGCAAGATGC TGCAGCACTA CCTGCAGAAC
    AAAAATGGGG AGGGCATCCC CTCAGCTGTC GCCCCCCGAG CTCAGCGGCC ACCCACAGCT
    GCCCCGGCTG CCCCCTCCTG CTCCTCCTCC AAGCAGCCTG CTCCCGACAC GGAGGCCTCG
    GAACAGCGGG CCTTGCGCAC CGTCCAGTAT GGCCTCCTGA GGATCCTCAG CAGGACCCTG
    GCCGCCCTGC GCCGCTTCAC CCCCGACGTC TGCCAGGTCC TGCTGGATCA GTCCCTGGAC
    CTTGCTGAGT ACAGCTTCCT GTTTGCCCTG AGCTTCACCA CTCCCACTTT TGACTCTGAA
    GGGGCCCCCT CCTTCGGGAC CCTTCTGGCC ACCGTGAATG TGGCCCTGAA CATGCTTGGA
    GAGCTGGACA AGAAAAAGGA GCCTCTCACC CAGGCTGTGG GGCTCAGCAC ACAAGCAGAA
    GGGACCAGAA CTTTGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065387.1
    CDS235..5175
    Translation

    Target ORF information:

    RefSeq Version XM_006065325.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nucleoporin 188kDa (NUP188), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGTGGTCCA GCCCTGCCTG GAGCAGTAGA GAACTGTGGA CAATTCTGCT AGGAAGGTCA 
    GCTCTTAGAG AACTGAATCA GATTGAGGCA GAGCTGAATA AACATTGGCA GCGGCTGTTA
    GAAGGACTTT CTTACTATAA ACCTCCCAGT CCAAGTTCAG CTGAAAAAGT GAAAGCTAGT
    AAAGATGTAG CTTCACCACT GAAGGAACTG GGTTTAAGAA TCAGCAAGTT TTTGGGTCTT
    GATGAAGAAC AGAGTGTGCA GTTACTCCAG TGTTACCTTC AAGAGGATTA CAGAGGTACT
    CGGGACTCAC TAAAGACGGT GCTGCAAGAT GAGAGGCAGA GCCAGGCCTT AATCCTGAAG
    ATTGCAGATT ATTACTATGA AGAAAGAACT TGTATTCTTC GTTGCGTCTT ACACCTTCTC
    ACTTATTTCC AAGATGAAAG ACACCCCTAT AGGGTTGAAT ATGCCGACTG TGTGGATAAA
    TTGGAAAAGG AGCTAGTTAC AAAATATAGA CAGCAGTTTG AAGAGCTTTA TAAAACTGAA
    GCACCAACGT GGGAGACACA TGGAAACCTC ATGACTGAGC GCCAGGTGTC TCGCTGGTTT
    GTTCAGTGCC TTCGGGAGCA GTCCATGCTG CTAGAGATTA TTTTCCTGTA TTATGCATAC
    TTTGAGATGG CACCTAGTGA CCTATTGGTG TTAACCAAGA TGTTTAAAGA GCAAGGATTT
    GGTAGTAGGC AGACTAACAG GCACCTGGTA GATGAGACCA TGGATCCTTT TGTGGATCGG
    ATTGGCTACT TCAGTGCCCT TATTCTGGTG GAAGGCATGG ACATTGAATC CTTGCATAAG
    TGTGCTTTGG ATGACAGAAG AGAACTGCAC CAGTTTGCCC AGGATGGACT TATTTGTCAG
    GATATGGACC GTTTGATGTT GACCTTTGGG GACATTCCGC ATCATGCCCC AGTGCTTTTG
    GCATGGGCCC TCCTCCGTCA CACCCTGAAT CCAGAAGAGA CGAGCAGTGT GGTCCGGAAG
    ATAGGGGGGA CAGCCATCCA ACTGAACGTC TTTCAGTACT TGACCCGACT GCTCCGCTCG
    CTGGCCAGTG GGGGAAATGA CTGCACCACC AGCACTGCCT GCATGTGTGT CTACGGACTC
    CTTTCTTTTG TCCTGACCTC CCTGGAGCTG CACACCCTGG GCAATCAGCA GGATGTAATT
    GACACAGCAT GTGAAGTCCT GGCAGACCCT TCTCTCCCAG AACTCTTCTG GGGAACAGAA
    CCAACCTCTG GTCTCGGGAT CATTCTGGAC AGTGTATGTG GAATGTTTCC CCATCTCCTC
    TCTCCACTCC TGCAGTTGCT TCGAGCCCTT GTATCAGGGA AGTCTACTGC TAAGAAGGTG
    TATAGTTTCT TGGATAAGAT GTCTTTCTAC AATGAACTCT ATAAGCACAA GCCCCATGAT
    GTGATCTCCC ATGAAGATGG AACCCTTTGG CGGAGGCAAA CACCCAAACT CCTTTACCCT
    CTAGGGGGTC AGACCAACCT TCGCATACCT CAAGGCACCG TGGGCCAAGT GATGCTGGAC
    GATAGGGCCT ACCTGGTGCG CTGGGAATAC TCGTACAGCA GCTGGACCCT CTTCACCTGC
    GAGATTGAGA TGCTGCTGCA TGTCGTTTCC ACTGCAGATG TGATTCAGCA CTGCCAGCGG
    GTCAAACCCA TCATCGACCT GGTCCATAAG GTCATCAGCA CAGACCTGTC GATAGCAGAC
    TGTCTCCTGC CCATCACGTC CCGCATCTAC ATGCTACTGC AGCGGTTAAC AACAGTCATC
    TCCCCGCCTG TGGATGTCAT TGCTTCTTGC GTCAATTGCT TGACTGTTTT GGCTGCCCGG
    AACCCAGCAA AGGTCTGGAC TGATCTTCTT CACACAGGCT TCTTGCCTTT CGTGGCCCAC
    CAGGTCTCCA GCATGAGTCA GATGATCAGT GCTGAGGGGA TGAATGCTGG TGGGTATGGA
    AACCTCTTGA TGAATAGTGA ACAGCCCCAG GGCGAGTATG GGGTCACTGT TGCCTTTCTG
    CGCTTGATCA CCACTCTTGT GAAGGGGCAG CTTGGTAGTA CCCAGAGCCA AGGGCTGGTG
    CCTTGTGTCA TGTTTGTGCT GAAGGAGATG CTGCCAAGTT ACCACAAGTG GCGCTACAAC
    TCCCATGGTG TGAGAGAACA GATTGGTTGC CTGATCCTGG AGCTGATTCA TTCAATACTG
    AACCTGTGCC ATGAGACAGA CCTGCATAGC AGTCACACCC CCAGCCTGCA GTCTCTCTGC
    ATCTGCAGCC TGGCCTACAC AGAAGCAGGA CAGACAGTAA TCAATATCAT GGGCATCGGT
    GTGGACACCA TCGACATGGT CATGGCTGCT CAGCCCCGCA GCGATGGGGC AGAGGGCCAG
    GGCCAGGGCC AGCTGCTGAT CAAGACAGTG AAATTGGCGT TCTCCGTCAC CAACAATGTC
    ATTCGGCTGA AACCGCCTTC TAATGTGGTG TCCCCTCTGG AACAGGCTCT CACACAGCAT
    GGTGCCCATG GGAACAACCT TATTGCTGTT CTTGCCAAAT ACATCTACCA CAAACACGAC
    CCAGCTCTGC CTCGTCTTGC CATCCAGCTG CTGAAACGTC TGGCCACGGT GGCCCCCATG
    TCAGTGTATG CCTGCCTGGG CAGTGATGCG GCTGCCATCC GAGACGCCTT CCTGACCCGA
    CTGCAGAGCA AGATTGAGGA TATGCGCATC AAAGTCATGA TTCTGGAATT CCTCACGGTT
    GCTGTAGAGA CGCAGCCAGG CCTCATTGAG CTGTTTCTGA ATCTGGAGGT GAAGGATGGC
    AGCGATGGCT CCAAGGAATT CAGCCTGGGG GTGTGGAGCT GTCTCCACGC GGTGCTGGAG
    CTGATCGGTT CCCAGCAGCA GGACCGCTAC TGGTGCCCCC CACTGCTGCA CCGGGCAGCC
    ATCGCCTTTT TGCACGCCCT GTGGCAGGAC CGGCGGGACA GTGCCATGCT GGTCCTCAGA
    ACCAAACCCA AGTTTTGGGA GAATCTAACG AGTCCGCTAT TTGGTACTCT TTCTCCTCCC
    TCGGAAACAT CTGAGCCCAG CATCCTGGAA ACCTGTGCCC TAATCATGAA GATAATTTGC
    TTGGAGATAT ACTATGTAGT TAAGGGCTCA TTAGACCAAT CATTAAAAGA TACACTCAAG
    AAATTTTCCA GCGAGAAGCG CTTTGCCTAC TGGTCGGGGT ATGTCAAGTC CTTGGCGGTT
    CACATGGCCG ACACAGAGGG CAGCAGCTGC ACCTCCCTGG TAGAGTATCA GATGCTGGTC
    TCTGCCTGGA GGATGTTTCT CATCATTGCC ACCAGTCACG CGGAGATAAC GCACCTGACA
    GACTCTGCAG TGCGTCGCCA GCTCTTTCTT GACGTGCTCG AGGGGACCAA GGCGTTACTC
    CTCGTCCCTA CCTCCGTGAA CTGCCTCCGC CTCGGCTCCA TGACGTGCAC CCTGCTGCTT
    ATTCTCCTCC GGCAGTGGAA AAGAGAGTTA GGGTCTGTGG ATGAAATCCT CGGGCCCCTG
    ACGGAGATCC TGGAGGGAGT GCTGCAGGCA GACCAGCAGC TTATGGAGAA GACCAAGGCC
    AAGGTGTTCT CAGCGTTCAT CACGGTGCTG CAGATGAAGG AGATGAGCGT GAATGACATC
    CCCCAGTACT CCCAGCTGGT GCTGAACGTC TGTGAGACCC TTCAGGAAGA GGTGATCGCA
    CTTTTCGACC ACACCCGCCA CAGCCTGGCG GCGGGCAGTG CCACCGAGGA CAAGGACAGC
    ATGGAGACCG ACGACTGCTC CCGGCTCCGG CACAAGGACC AGCGGGACGG GGTCTGTGTC
    CTGGGCCTGC ACCTGGCCAA GGAGCTGTGT GAGGTGGATG AGGACGGGGA CTCGTGGCTG
    CAGGTGACCC GCCGGCTCCC CATCCTGCCG ACCCTCTTCA CCACCTTGGA GGTGAGCCTG
    CGCGTGAAGC AGAACCTGCA CTTCACCGAG GCGGCGCTGC ACCTGCTCCT CACGCTGGCC
    CGCACCCAGC AGGGGGCCAC AGCCGTGGCC GGGGCCGGCG TCACCCAGAG CATTTGCTTG
    CCTCTCCTGA GTGTGTACCA GCTCAGCGCC AACGGGACAG TGCAGACACC CGGCACCTCT
    CGGAAGTCCC TGGACGCCCC CTCTTGGCCT GGCGTCTACC GTCTCTCCAT GTCCTTGATG
    GAGCGGCTCC TCAAAACCCT GCGCTACAAC TTCCTGACCG AGGCCCTGGA CTTCGTGGGG
    GTTCACCAGG AGCGGACCTT ACAGTGCCTC AATGCCGTGA GGACGGTGCA GAGTCTGGCG
    TGCCTGGAGG AGGCTGACCA CACCGTGGGC TTCCTCCTAC AGCTCTCCAG CTTCAGGAAG
    GAGTGGCACT TCCACCTGCC TCAGCTCATG CGGGATGTCC AGGTGAACCT CGGCTACCTG
    TGCCAGGCCT GCACCTCCCT CCTGCACAGC CGCAAGATGC TGCAGCACTA CCTGCAGAAC
    AAAAATGGGG AGGGCATCCC CTCAGCTGTC GCCCCCCGAG CTCAGCGGCC ACCCACAGCT
    GCCCCGGCTG CCCCCTCCTG CTCCTCCTCC AAGCAGCCTG CTCCCGACAC GGAGGCCTCG
    GAACAGCGGG CCTTGCGCAC CGTCCAGTAT GGCCTCCTGA GGATCCTCAG CAGGACCCTG
    GCCGCCCTGC GCCGCTTCAC CCCCGACGTC TGCCAGGTCC TGCTGGATCA GTCCCTGGAC
    CTTGCTGAGT ACAGCTTCCT GTTTGCCCTG AGCTTCACCA CTCCCACTTT TGACTCTGAA
    GGGGCCCCCT CCTTCGGGAC CCTTCTGGCC ACCGTGAATG TGGCCCTGAA CATGCTTGGA
    GAGCTGGACA AGAAAAAGGA GCCTCTCACC CAGGCTGTGG GGCTCAGCAC ACAAGCAGAA
    GGGACCAGAA CTTTGAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu46690
    Clone ID Related Accession (Same CDS sequence) XM_025261803.1
    Accession Version XM_025261803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nucleoporin NUP188 homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCTG CCGCCGGCGG TCCGTGTGTG AGGAGCAGTA GAGAACTGTG GACAATTCTG 
    CTAGGAAGGT CAGCTCTTAG AGAACTGAAT CAGATTGAGG CAGAGCTGAA TAAACATTGG
    CAGCGGCTGT TAGAAGGACT TTCTTACTAT AAACCTCCCA GTCCAAGTTC AGCTGAAAAA
    GTGAAAGCTA GTAAAGATGT AGCTTCACCA CTGAAGGAAC TGGGTTTAAG AATCAGCAAG
    TTTTTGGGTC TTGATGAAGA ACAGAGTGTG CAGTTACTCC AGTGTTACCT TCAAGAGGAT
    TACAGAGGTA CTCGGGACTC ACTAAAGACG GTGCTGCAAG ATGAGAGGCA GAGCCAGGCC
    TTAATCCTGA AGATTGCAGA TTATTACTAT GAAGAAAGAA CTTGTATTCT TCGTTGCGTC
    TTACACCTTC TCACTTATTT CCAAGATGAA AGACACCCCT ATAGGGTTGA ATATGCCGAC
    TGTGTGGATA AATTGGAAAA GGAGCTAGTT ACAAAATATA GACAGCAGTT TGAAGAGCTT
    TATAAAACTG AAGCACCAAC GTGGGAGACA CATGGAAACC TCATGACTGA GCGCCAGGTG
    TCTCGCTGGT TTGTTCAGTG CCTTCGGGAG CAGTCCATGC TGCTAGAGAT TATTTTCCTG
    TATTATGCAT ACTTTGAGAT GGCACCTAGT GACCTATTGG TGTTAACCAA GATGTTTAAA
    GAGCAAGGAT TTGGTAGTAG GCAGACTAAC AGGCACCTGG TAGATGAGAC CATGGATCCT
    TTTGTGGATC GGATTGGCTA CTTCAGTGCC CTTATTCTGG TGGAAGGCAT GGACATTGAA
    TCCTTGCATA AGTGTGCTTT GGATGACAGA AGAGAACTGC ACCAGTTTGC CCAGGATGGA
    CTTATTTGTC AGGATATGGA CCGTTTGATG TTGACCTTTG GGGACATTCC GCATCATGCC
    CCAGTGCTTT TGGCATGGGC CCTCCTCCGT CACACCCTGA ATCCAGAAGA GACGAGCAGT
    GTGGTCCGGA AGATAGGGGG GACAGCCATC CAACTGAACG TCTTTCAGTA CTTGACCCGA
    CTGCTCCGCT CGCTGGCCAG TGGGGGAAAT GACTGCACCA CCAGCACTGC CTGCATGTGT
    GTCTACGGAC TCCTTTCTTT TGTCCTGACC TCCCTGGAGC TGCACACCCT GGGCAATCAG
    CAGGATGTAA TTGACACAGC ATGTGAAGTC CTGGCAGACC CTTCTCTCCC AGAACTCTTC
    TGGGGAACAG AACCAACCTC TGGTCTCGGG ATCATTCTGG ACAGTGTATG TGGAATGTTT
    CCCCATCTCC TCTCTCCACT CCTGCAGTTG CTTCGAGCCC TTGTATCAGG GAAGTCTACT
    GCTAAGAAGG TGTATAGTTT CTTGGATAAG ATGTCTTTCT ACAATGAACT CTATAAGCAC
    AAGCCCCATG ATGTGATCTC CCATGAAGAT GGAACCCTTT GGCGGAGGCA AACACCCAAA
    CTCCTTTACC CTCTAGGGGG TCAGACCAAC CTTCGCATAC CTCAAGGCAC CGTGGGCCAA
    GTGATGCTGG ACGATAGGGC CTACCTGGTG CGCTGGGAAT ACTCGTACAG CAGCTGGACC
    CTCTTCACCT GCGAGATTGA GATGCTGCTG CATGTCGTTT CCACTGCAGA TGTGATTCAG
    CACTGCCAGC GGGTCAAACC CATCATCGAC CTGGTCCATA AGGTCATCAG CACAGACCTG
    TCGATAGCAG ACTGTCTCCT GCCCATCACG TCCCGCATCT ACATGCTACT GCAGCGGTTA
    ACAACAGTCA TCTCCCCGCC TGTGGATGTC ATTGCTTCTT GCGTCAATTG CTTGACTGTT
    TTGGCTGCCC GGAACCCAGC AAAGGTCTGG ACTGATCTTC TTCACACAGG CTTCTTGCCT
    TTCGTGGCCC ACCAGGTCTC CAGCATGAGT CAGATGATCA GTGCTGAGGG GATGAATGCT
    GGTGGGTATG GAAACCTCTT GATGAATAGT GAACAGCCCC AGGGCGAGTA TGGGGTCACT
    GTTGCCTTTC TGCGCTTGAT CACCACTCTT GTGAAGGGGC AGCTTGGTAG TACCCAGAGC
    CAAGGGCTGG TGCCTTGTGT CATGTTTGTG CTGAAGGAGA TGCTGCCAAG TTACCACAAG
    TGGCGCTACA ACTCCCATGG TGTGAGAGAA CAGATTGGTT GCCTGATCCT GGAGCTGATT
    CATTCAATAC TGAACCTGTG CCATGAGACA GACCTGCATA GCAGTCACAC CCCCAGCCTG
    CAGTCTCTCT GCATCTGCAG CCTGGCCTAC ACAGAAGCAG GACAGACAGT AATCAATATC
    ATGGGCATCG GTGTGGACAC CATCGACATG GTCATGGCTG CTCAGCCCCG CAGCGATGGG
    GCAGAGGGCC AGGGCCAGGG CCAGCTGCTG ATCAAGACAG TGAAATTGGC GTTCTCCGTC
    ACCAACAATG TCATTCGGCT GAAACCGCCT TCTAATGTGG TGTCCCCTCT GGAACAGGCT
    CTCACACAGC ATGGTGCCCA TGGGAACAAC CTTATTGCTG TTCTTGCCAA ATACATCTAC
    CACAAACACG ACCCAGCTCT GCCTCGTCTT GCCATCCAGC TGCTGAAACG TCTGGCCACG
    GTGGCCCCCA TGTCAGTGTA TGCCTGCCTG GGCAGTGATG CGGCTGCCAT CCGAGACGCC
    TTCCTGACCC GACTGCAGAG CAAGATTGAG GATATGCGCA TCAAAGTCAT GATTCTGGAA
    TTCCTCACGG TTGCTGTAGA GACGCAGCCA GGCCTCATTG AGCTGTTTCT GAATCTGGAG
    GTGAAGGATG GCAGCGATGG CTCCAAGGAA TTCAGCCTGG GGGTGTGGAG CTGTCTCCAC
    GCGGTGCTGG AGCTGATCGG TTCCCAGCAG CAGGACCGCT ACTGGTGCCC CCCACTGCTG
    CACCGGGCAG CCATCGCCTT TTTGCACGCC CTGTGGCAGG ACCGGCGGGA CAGTGCCATG
    CTGGTCCTCA GAACCAAACC CAAGTTTTGG GAGAATCTAA CGAGTCCGCT ATTTGGTACT
    CTTTCTCCTC CCTCGGAAAC ATCTGAGCCC AGCATCCTGG AAACCTGTGC CCTAATCATG
    AAGATAATTT GCTTGGAGAT ATACTATGTA GTTAAGGGCT CATTAGACCA ATCATTAAAA
    GATACACTCA AGAAATTTTC CAGCGAGAAG CGCTTTGCCT ACTGGTCGGG GTATGTCAAG
    TCCTTGGCGG TTCACATGGC CGACACAGAG GGCAGCAGCT GCACCTCCCT GGTAGAGTAT
    CAGATGCTGG TCTCTGCCTG GAGGATGTTT CTCATCATTG CCACCAGTCA CGCGGAGATA
    ACGCACCTGA CAGACTCTGC AGTGCGTCGC CAGCTCTTTC TTGACGTGCT CGAGGGGACC
    AAGGCGTTAC TCCTCGTCCC TACCTCCGTG AACTGCCTCC GCCTCGGCTC CATGACGTGC
    ACCCTGCTGC TTATTCTCCT CCGGCAGTGG AAAAGAGAGT TAGGGTCTGT GGATGAAATC
    CTCGGGCCCC TGACGGAGAT CCTGGAGGGA GTGCTGCAGG CAGACCAGCA GCTTATGGAG
    AAGACCAAGG CCAAGGTGTT CTCAGCGTTC ATCACGGTGC TGCAGATGAA GGAGATGAGC
    GTGAATGACA TCCCCCAGTA CTCCCAGCTG GTGCTGAACG TCTGTGAGAC CCTTCAGGAA
    GAGGTGATCG CACTTTTCGA CCACACCCGC CACAGCCTGG CGGCGGGCAG TGCCACCGAG
    GACAAGGACA GCATGGAGAC CGACGACTGC TCCCGGCTCC GGCACAAGGA CCAGCGGGAC
    GGGGTCTGTG TCCTGGGCCT GCACCTGGCC AAGGAGCTGT GTGAGGTGGA TGAGGACGGG
    GACTCGTGGC TGCAGGTGAC CCGCCGGCTC CCCATCCTGC CGACCCTCTT CACCACCTTG
    GAGGTGAGCC TGCGCGTGAA GCAGAACCTG CACTTCACCG AGGCGGCGCT GCACCTGCTC
    CTCACGCTGG CCCGCACCCA GCAGGGGGCC ACAGCCGTGG CCGGGGCCGG CGTCACCCAG
    AGCATTTGCT TGCCTCTCCT GAGTGTGTAC CAGCTCAGCG CCAACGGGAC AGTGCAGACA
    CCCGGCACCT CTCGGAAGTC CCTGGACGCC CCCTCTTGGC CTGGCGTCTA CCGTCTCTCC
    ATGTCCTTGA TGGAGCGGCT CCTCAAAACC CTGCGCTACA ACTTCCTGAC CGAGGCCCTG
    GACTTCGTGG GGGTTCACCA GGAGCGGACC TTACAGTGCC TCAATGCCGT GAGGACGGTG
    CAGAGTCTGG CGTGCCTGGA GGAGGCTGAC CACACCGTGG GCTTCCTCCT ACAGCTCTCC
    AGCTTCAGGA AGGAGTGGCA CTTCCACCTG CCTCAGCTCA TGCGGGATGT CCAGGTGAAC
    CTCGGCTACC TGTGCCAGGC CTGCACCTCC CTCCTGCACA GCCGCAAGAT GCTGCAGCAC
    TACCTGCAGA ACAAAAATGG GGAGGGCATC CCCTCAGCTG TCGCCCCCCG AGCTCAGCGG
    CCACCCACAG CTGCCCCGGC TGCCCCCTCC TGCTCCTCCT CCAAGCAGCC TGCTCCCGAC
    ACGGAGGCCT CGGAACAGCG GGCCTTGCGC ACCGTCCAGT ATGGCCTCCT GAGGATCCTC
    AGCAGGACCC TGGCCGCCCT GCGCCGCTTC ACCCCCGACG TCTGCCAGGT CCTGCTGGAT
    CAGTCTCATC TTGCTTTGCA GTCCCTGGAC CTTGCTGAGT ACAGCTTCCT GTTTGCCCTG
    AGCTTCACCA CTCCCACTTT TGACTCTGAA GGGGCCCCCT CCTTCGGGAC CCTTCTGGCC
    ACCGTGAATG TGGCCCTGAA CATGCTTGGA GAGCTGGACA AGAAAAAGGA GCCTCTCACC
    CAGGCTGTGG GGCTCAGCAC ACAAGCAGAA GGGACCAGAA CTTTGAAGTC GCTCCTGATG
    TTCACCATGG AGAACTGCTT CTACCTGCTC ATCTCCCAGG CGATGCGTTA CCTTCGGGAC
    CCGGCTGTGC ACCCCCGGGA CAAGCAGCGG ATGAAGCAGG AGCTCAGCTC AGAGTTGAGC
    ACTCTCCTCT CCAGCCTCTC CCGCTACTTC CGCCGGGGAG CCCCCAGCTC CCCTGCTGCT
    GGCGTCCTTC CCTCGCCTCA GGGCAAGTCC ACCTCTCTCT CCAAGGTCAG CCCTGAGAGT
    CAGGAGCCTC TGATCCAGCT GGTGCAGGCC TTTGTCCGAC ACGTGCAAAG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025117588.1
    CDS49..5322
    Translation

    Target ORF information:

    RefSeq Version XM_025261803.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nucleoporin 188 (NUP188), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025261803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCTG CCGCCGGCGG TCCGTGTGTG AGGAGCAGTA GAGAACTGTG GACAATTCTG 
    CTAGGAAGGT CAGCTCTTAG AGAACTGAAT CAGATTGAGG CAGAGCTGAA TAAACATTGG
    CAGCGGCTGT TAGAAGGACT TTCTTACTAT AAACCTCCCA GTCCAAGTTC AGCTGAAAAA
    GTGAAAGCTA GTAAAGATGT AGCTTCACCA CTGAAGGAAC TGGGTTTAAG AATCAGCAAG
    TTTTTGGGTC TTGATGAAGA ACAGAGTGTG CAGTTACTCC AGTGTTACCT TCAAGAGGAT
    TACAGAGGTA CTCGGGACTC ACTAAAGACG GTGCTGCAAG ATGAGAGGCA GAGCCAGGCC
    TTAATCCTGA AGATTGCAGA TTATTACTAT GAAGAAAGAA CTTGTATTCT TCGTTGCGTC
    TTACACCTTC TCACTTATTT CCAAGATGAA AGACACCCCT ATAGGGTTGA ATATGCCGAC
    TGTGTGGATA AATTGGAAAA GGAGCTAGTT ACAAAATATA GACAGCAGTT TGAAGAGCTT
    TATAAAACTG AAGCACCAAC GTGGGAGACA CATGGAAACC TCATGACTGA GCGCCAGGTG
    TCTCGCTGGT TTGTTCAGTG CCTTCGGGAG CAGTCCATGC TGCTAGAGAT TATTTTCCTG
    TATTATGCAT ACTTTGAGAT GGCACCTAGT GACCTATTGG TGTTAACCAA GATGTTTAAA
    GAGCAAGGAT TTGGTAGTAG GCAGACTAAC AGGCACCTGG TAGATGAGAC CATGGATCCT
    TTTGTGGATC GGATTGGCTA CTTCAGTGCC CTTATTCTGG TGGAAGGCAT GGACATTGAA
    TCCTTGCATA AGTGTGCTTT GGATGACAGA AGAGAACTGC ACCAGTTTGC CCAGGATGGA
    CTTATTTGTC AGGATATGGA CCGTTTGATG TTGACCTTTG GGGACATTCC GCATCATGCC
    CCAGTGCTTT TGGCATGGGC CCTCCTCCGT CACACCCTGA ATCCAGAAGA GACGAGCAGT
    GTGGTCCGGA AGATAGGGGG GACAGCCATC CAACTGAACG TCTTTCAGTA CTTGACCCGA
    CTGCTCCGCT CGCTGGCCAG TGGGGGAAAT GACTGCACCA CCAGCACTGC CTGCATGTGT
    GTCTACGGAC TCCTTTCTTT TGTCCTGACC TCCCTGGAGC TGCACACCCT GGGCAATCAG
    CAGGATGTAA TTGACACAGC ATGTGAAGTC CTGGCAGACC CTTCTCTCCC AGAACTCTTC
    TGGGGAACAG AACCAACCTC TGGTCTCGGG ATCATTCTGG ACAGTGTATG TGGAATGTTT
    CCCCATCTCC TCTCTCCACT CCTGCAGTTG CTTCGAGCCC TTGTATCAGG GAAGTCTACT
    GCTAAGAAGG TGTATAGTTT CTTGGATAAG ATGTCTTTCT ACAATGAACT CTATAAGCAC
    AAGCCCCATG ATGTGATCTC CCATGAAGAT GGAACCCTTT GGCGGAGGCA AACACCCAAA
    CTCCTTTACC CTCTAGGGGG TCAGACCAAC CTTCGCATAC CTCAAGGCAC CGTGGGCCAA
    GTGATGCTGG ACGATAGGGC CTACCTGGTG CGCTGGGAAT ACTCGTACAG CAGCTGGACC
    CTCTTCACCT GCGAGATTGA GATGCTGCTG CATGTCGTTT CCACTGCAGA TGTGATTCAG
    CACTGCCAGC GGGTCAAACC CATCATCGAC CTGGTCCATA AGGTCATCAG CACAGACCTG
    TCGATAGCAG ACTGTCTCCT GCCCATCACG TCCCGCATCT ACATGCTACT GCAGCGGTTA
    ACAACAGTCA TCTCCCCGCC TGTGGATGTC ATTGCTTCTT GCGTCAATTG CTTGACTGTT
    TTGGCTGCCC GGAACCCAGC AAAGGTCTGG ACTGATCTTC TTCACACAGG CTTCTTGCCT
    TTCGTGGCCC ACCAGGTCTC CAGCATGAGT CAGATGATCA GTGCTGAGGG GATGAATGCT
    GGTGGGTATG GAAACCTCTT GATGAATAGT GAACAGCCCC AGGGCGAGTA TGGGGTCACT
    GTTGCCTTTC TGCGCTTGAT CACCACTCTT GTGAAGGGGC AGCTTGGTAG TACCCAGAGC
    CAAGGGCTGG TGCCTTGTGT CATGTTTGTG CTGAAGGAGA TGCTGCCAAG TTACCACAAG
    TGGCGCTACA ACTCCCATGG TGTGAGAGAA CAGATTGGTT GCCTGATCCT GGAGCTGATT
    CATTCAATAC TGAACCTGTG CCATGAGACA GACCTGCATA GCAGTCACAC CCCCAGCCTG
    CAGTCTCTCT GCATCTGCAG CCTGGCCTAC ACAGAAGCAG GACAGACAGT AATCAATATC
    ATGGGCATCG GTGTGGACAC CATCGACATG GTCATGGCTG CTCAGCCCCG CAGCGATGGG
    GCAGAGGGCC AGGGCCAGGG CCAGCTGCTG ATCAAGACAG TGAAATTGGC GTTCTCCGTC
    ACCAACAATG TCATTCGGCT GAAACCGCCT TCTAATGTGG TGTCCCCTCT GGAACAGGCT
    CTCACACAGC ATGGTGCCCA TGGGAACAAC CTTATTGCTG TTCTTGCCAA ATACATCTAC
    CACAAACACG ACCCAGCTCT GCCTCGTCTT GCCATCCAGC TGCTGAAACG TCTGGCCACG
    GTGGCCCCCA TGTCAGTGTA TGCCTGCCTG GGCAGTGATG CGGCTGCCAT CCGAGACGCC
    TTCCTGACCC GACTGCAGAG CAAGATTGAG GATATGCGCA TCAAAGTCAT GATTCTGGAA
    TTCCTCACGG TTGCTGTAGA GACGCAGCCA GGCCTCATTG AGCTGTTTCT GAATCTGGAG
    GTGAAGGATG GCAGCGATGG CTCCAAGGAA TTCAGCCTGG GGGTGTGGAG CTGTCTCCAC
    GCGGTGCTGG AGCTGATCGG TTCCCAGCAG CAGGACCGCT ACTGGTGCCC CCCACTGCTG
    CACCGGGCAG CCATCGCCTT TTTGCACGCC CTGTGGCAGG ACCGGCGGGA CAGTGCCATG
    CTGGTCCTCA GAACCAAACC CAAGTTTTGG GAGAATCTAA CGAGTCCGCT ATTTGGTACT
    CTTTCTCCTC CCTCGGAAAC ATCTGAGCCC AGCATCCTGG AAACCTGTGC CCTAATCATG
    AAGATAATTT GCTTGGAGAT ATACTATGTA GTTAAGGGCT CATTAGACCA ATCATTAAAA
    GATACACTCA AGAAATTTTC CAGCGAGAAG CGCTTTGCCT ACTGGTCGGG GTATGTCAAG
    TCCTTGGCGG TTCACATGGC CGACACAGAG GGCAGCAGCT GCACCTCCCT GGTAGAGTAT
    CAGATGCTGG TCTCTGCCTG GAGGATGTTT CTCATCATTG CCACCAGTCA CGCGGAGATA
    ACGCACCTGA CAGACTCTGC AGTGCGTCGC CAGCTCTTTC TTGACGTGCT CGAGGGGACC
    AAGGCGTTAC TCCTCGTCCC TACCTCCGTG AACTGCCTCC GCCTCGGCTC CATGACGTGC
    ACCCTGCTGC TTATTCTCCT CCGGCAGTGG AAAAGAGAGT TAGGGTCTGT GGATGAAATC
    CTCGGGCCCC TGACGGAGAT CCTGGAGGGA GTGCTGCAGG CAGACCAGCA GCTTATGGAG
    AAGACCAAGG CCAAGGTGTT CTCAGCGTTC ATCACGGTGC TGCAGATGAA GGAGATGAGC
    GTGAATGACA TCCCCCAGTA CTCCCAGCTG GTGCTGAACG TCTGTGAGAC CCTTCAGGAA
    GAGGTGATCG CACTTTTCGA CCACACCCGC CACAGCCTGG CGGCGGGCAG TGCCACCGAG
    GACAAGGACA GCATGGAGAC CGACGACTGC TCCCGGCTCC GGCACAAGGA CCAGCGGGAC
    GGGGTCTGTG TCCTGGGCCT GCACCTGGCC AAGGAGCTGT GTGAGGTGGA TGAGGACGGG
    GACTCGTGGC TGCAGGTGAC CCGCCGGCTC CCCATCCTGC CGACCCTCTT CACCACCTTG
    GAGGTGAGCC TGCGCGTGAA GCAGAACCTG CACTTCACCG AGGCGGCGCT GCACCTGCTC
    CTCACGCTGG CCCGCACCCA GCAGGGGGCC ACAGCCGTGG CCGGGGCCGG CGTCACCCAG
    AGCATTTGCT TGCCTCTCCT GAGTGTGTAC CAGCTCAGCG CCAACGGGAC AGTGCAGACA
    CCCGGCACCT CTCGGAAGTC CCTGGACGCC CCCTCTTGGC CTGGCGTCTA CCGTCTCTCC
    ATGTCCTTGA TGGAGCGGCT CCTCAAAACC CTGCGCTACA ACTTCCTGAC CGAGGCCCTG
    GACTTCGTGG GGGTTCACCA GGAGCGGACC TTACAGTGCC TCAATGCCGT GAGGACGGTG
    CAGAGTCTGG CGTGCCTGGA GGAGGCTGAC CACACCGTGG GCTTCCTCCT ACAGCTCTCC
    AGCTTCAGGA AGGAGTGGCA CTTCCACCTG CCTCAGCTCA TGCGGGATGT CCAGGTGAAC
    CTCGGCTACC TGTGCCAGGC CTGCACCTCC CTCCTGCACA GCCGCAAGAT GCTGCAGCAC
    TACCTGCAGA ACAAAAATGG GGAGGGCATC CCCTCAGCTG TCGCCCCCCG AGCTCAGCGG
    CCACCCACAG CTGCCCCGGC TGCCCCCTCC TGCTCCTCCT CCAAGCAGCC TGCTCCCGAC
    ACGGAGGCCT CGGAACAGCG GGCCTTGCGC ACCGTCCAGT ATGGCCTCCT GAGGATCCTC
    AGCAGGACCC TGGCCGCCCT GCGCCGCTTC ACCCCCGACG TCTGCCAGGT CCTGCTGGAT
    CAGTCTCATC TTGCTTTGCA GTCCCTGGAC CTTGCTGAGT ACAGCTTCCT GTTTGCCCTG
    AGCTTCACCA CTCCCACTTT TGACTCTGAA GGGGCCCCCT CCTTCGGGAC CCTTCTGGCC
    ACCGTGAATG TGGCCCTGAA CATGCTTGGA GAGCTGGACA AGAAAAAGGA GCCTCTCACC
    CAGGCTGTGG GGCTCAGCAC ACAAGCAGAA GGGACCAGAA CTTTGAAGTC GCTCCTGATG
    TTCACCATGG AGAACTGCTT CTACCTGCTC ATCTCCCAGG CGATGCGTTA CCTTCGGGAC
    CCGGCTGTGC ACCCCCGGGA CAAGCAGCGG ATGAAGCAGG AGCTCAGCTC AGAGTTGAGC
    ACTCTCCTCT CCAGCCTCTC CCGCTACTTC CGCCGGGGAG CCCCCAGCTC CCCTGCTGCT
    GGCGTCCTTC CCTCGCCTCA GGGCAAGTCC ACCTCTCTCT CCAAGGTCAG CCCTGAGAGT
    CAGGAGCCTC TGATCCAGCT GGTGCAGGCC TTTGTCCGAC ACGTGCAAAG ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu46691
    Clone ID Related Accession (Same CDS sequence) XM_025261804.1
    Accession Version XM_025261804.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nucleoporin NUP188 homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCTG CCGCCGGCGG TCCGTGTGTG AGGAGCAGTA GAGAACTGTG GACAATTCTG 
    CTAGGAAGGT CAGCTCTTAG AGAACTGAAT CAGATTGAGG CAGAGCTGAA TAAACATTGG
    CAGCGGCTGT TAGAAGGACT TTCTTACTAT AAACCTCCCA GTCCAAGTTC AGCTGAAAAA
    GTGAAAGCTA GTAAAGATGT AGCTTCACCA CTGAAGGAAC TGGGTTTAAG AATCAGCAAG
    TTTTTGGGTC TTGATGAAGA ACAGAGTGTG CAGTTACTCC AGTGTTACCT TCAAGAGGAT
    TACAGAGGTA CTCGGGACTC ACTAAAGACG GTGCTGCAAG ATGAGAGGCA GAGCCAGGCC
    TTAATCCTGA AGATTGCAGA TTATTACTAT GAAGAAAGAA CTTGTATTCT TCGTTGCGTC
    TTACACCTTC TCACTTATTT CCAAGATGAA AGACACCCCT ATAGGGTTGA ATATGCCGAC
    TGTGTGGATA AATTGGAAAA GGAGCTAGTT ACAAAATATA GACAGCAGTT TGAAGAGCTT
    TATAAAACTG AAGCACCAAC GTGGGAGACA CATGGAAACC TCATGACTGA GCGCCAGGTG
    TCTCGCTGGT TTGTTCAGTG CCTTCGGGAG CAGTCCATGC TGCTAGAGAT TATTTTCCTG
    TATTATGCAT ACTTTGAGAT GGCACCTAGT GACCTATTGG TGTTAACCAA GATGTTTAAA
    GAGCAAGGAT TTGGTAGTAG GCAGACTAAC AGGCACCTGG TAGATGAGAC CATGGATCCT
    TTTGTGGATC GGATTGGCTA CTTCAGTGCC CTTATTCTGG TGGAAGGCAT GGACATTGAA
    TCCTTGCATA AGTGTGCTTT GGATGACAGA AGAGAACTGC ACCAGTTTGC CCAGGATGGA
    CTTATTTGTC AGGATATGGA CCGTTTGATG TTGACCTTTG GGGACATTCC GCATCATGCC
    CCAGTGCTTT TGGCATGGGC CCTCCTCCGT CACACCCTGA ATCCAGAAGA GACGAGCAGT
    GTGGTCCGGA AGATAGGGGG GACAGCCATC CAACTGAACG TCTTTCAGTA CTTGACCCGA
    CTGCTCCGCT CGCTGGCCAG TGGGGGAAAT GACTGCACCA CCAGCACTGC CTGCATGTGT
    GTCTACGGAC TCCTTTCTTT TGTCCTGACC TCCCTGGAGC TGCACACCCT GGGCAATCAG
    CAGGATGTAA TTGACACAGC ATGTGAAGTC CTGGCAGACC CTTCTCTCCC AGAACTCTTC
    TGGGGAACAG AACCAACCTC TGGTCTCGGG ATCATTCTGG ACAGTGTATG TGGAATGTTT
    CCCCATCTCC TCTCTCCACT CCTGCAGTTG CTTCGAGCCC TTGTATCAGG GAAGTCTACT
    GCTAAGAAGG TGTATAGTTT CTTGGATAAG ATGTCTTTCT ACAATGAACT CTATAAGCAC
    AAGCCCCATG ATGTGATCTC CCATGAAGAT GGAACCCTTT GGCGGAGGCA AACACCCAAA
    CTCCTTTACC CTCTAGGGGG TCAGACCAAC CTTCGCATAC CTCAAGGCAC CGTGGGCCAA
    GTGATGCTGG ACGATAGGGC CTACCTGGTG CGCTGGGAAT ACTCGTACAG CAGCTGGACC
    CTCTTCACCT GCGAGATTGA GATGCTGCTG CATGTCGTTT CCACTGCAGA TGTGATTCAG
    CACTGCCAGC GGGTCAAACC CATCATCGAC CTGGTCCATA AGGTCATCAG CACAGACCTG
    TCGATAGCAG ACTGTCTCCT GCCCATCACG TCCCGCATCT ACATGCTACT GCAGCGGTTA
    ACAACAGTCA TCTCCCCGCC TGTGGATGTC ATTGCTTCTT GCGTCAATTG CTTGACTGTT
    TTGGCTGCCC GGAACCCAGC AAAGGTCTGG ACTGATCTTC TTCACACAGG CTTCTTGCCT
    TTCGTGGCCC ACCAGGTCTC CAGCATGAGT CAGATGATCA GTGCTGAGGG GATGAATGCT
    GGTGGGTATG GAAACCTCTT GATGAATAGT GAACAGCCCC AGGGCGAGTA TGGGGTCACT
    GTTGCCTTTC TGCGCTTGAT CACCACTCTT GTGAAGGGGC AGCTTGGTAG TACCCAGAGC
    CAAGGGCTGG TGCCTTGTGT CATGTTTGTG CTGAAGGAGA TGCTGCCAAG TTACCACAAG
    TGGCGCTACA ACTCCCATGG TGTGAGAGAA CAGATTGGTT GCCTGATCCT GGAGCTGATT
    CATTCAATAC TGAACCTGTG CCATGAGACA GACCTGCATA GCAGTCACAC CCCCAGCCTG
    CAGTCTCTCT GCATCTGCAG CCTGGCCTAC ACAGAAGCAG GACAGACAGT AATCAATATC
    ATGGGCATCG GTGTGGACAC CATCGACATG GTCATGGCTG CTCAGCCCCG CAGCGATGGG
    GCAGAGGGCC AGGGCCAGGG CCAGCTGCTG ATCAAGACAG TGAAATTGGC GTTCTCCGTC
    ACCAACAATG TCATTCGGCT GAAACCGCCT TCTAATGTGG TGTCCCCTCT GGAACAGGCT
    CTCACACAGC ATGGTGCCCA TGGGAACAAC CTTATTGCTG TTCTTGCCAA ATACATCTAC
    CACAAACACG ACCCAGCTCT GCCTCGTCTT GCCATCCAGC TGCTGAAACG TCTGGCCACG
    GTGGCCCCCA TGTCAGTGTA TGCCTGCCTG GGCAGTGATG CGGCTGCCAT CCGAGACGCC
    TTCCTGACCC GACTGCAGAG CAAGATTGAG GATATGCGCA TCAAAGTCAT GATTCTGGAA
    TTCCTCACGG TTGCTGTAGA GACGCAGCCA GGCCTCATTG AGCTGTTTCT GAATCTGGAG
    GTGAAGGATG GCAGCGATGG CTCCAAGGAA TTCAGCCTGG GGGTGTGGAG CTGTCTCCAC
    GCGGTGCTGG AGCTGATCGG TTCCCAGCAG CAGGACCGCT ACTGGTGCCC CCCACTGCTG
    CACCGGGCAG CCATCGCCTT TTTGCACGCC CTGTGGCAGG ACCGGCGGGA CAGTGCCATG
    CTGGTCCTCA GAACCAAACC CAAGTTTTGG GAGAATCTAA CGAGTCCGCT ATTTGGTACT
    CTTTCTCCTC CCTCGGAAAC ATCTGAGCCC AGCATCCTGG AAACCTGTGC CCTAATCATG
    AAGATAATTT GCTTGGAGAT ATACTATGTA GTTAAGGGCT CATTAGACCA ATCATTAAAA
    GATACACTCA AGAAATTTTC CAGCGAGAAG CGCTTTGCCT ACTGGTCGGG GTATGTCAAG
    TCCTTGGCGG TTCACATGGC CGACACAGAG GGCAGCAGCT GCACCTCCCT GGTAGAGTAT
    CAGATGCTGG TCTCTGCCTG GAGGATGTTT CTCATCATTG CCACCAGTCA CGCGGAGATA
    ACGCACCTGA CAGACTCTGC AGTGCGTCGC CAGCTCTTTC TTGACGTGCT CGAGGGGACC
    AAGGCGTTAC TCCTCGTCCC TACCTCCGTG AACTGCCTCC GCCTCGGCTC CATGACGTGC
    ACCCTGCTGC TTATTCTCCT CCGGCAGTGG AAAAGAGAGT TAGGGTCTGT GGATGAAATC
    CTCGGGCCCC TGACGGAGAT CCTGGAGGGA GTGCTGCAGG CAGACCAGCA GCTTATGGAG
    AAGACCAAGG CCAAGGTGTT CTCAGCGTTC ATCACGGTGC TGCAGATGAA GGAGATGAGC
    GTGAATGACA TCCCCCAGTA CTCCCAGCTG GTGCTGAACG TCTGTGAGAC CCTTCAGGAA
    GAGGTGATCG CACTTTTCGA CCACACCCGC CACAGCCTGG CGGCGGGCAG TGCCACCGAG
    GACAAGGACA GCATGGAGAC CGACGACTGC TCCCGGCTCC GGCACAAGGA CCAGCGGGAC
    GGGGTCTGTG TCCTGGGCCT GCACCTGGCC AAGGAGCTGT GTGAGGTGGA TGAGGACGGG
    GACTCGTGGC TGCAGGTGAC CCGCCGGCTC CCCATCCTGC CGACCCTCTT CACCACCTTG
    GAGGTGAGCC TGCGCGTGAA GCAGAACCTG CACTTCACCG AGGCGGCGCT GCACCTGCTC
    CTCACGCTGG CCCGCACCCA GCAGGGGGCC ACAGCCGTGG CCGGGGCCGG CGTCACCCAG
    AGCATTTGCT TGCCTCTCCT GAGTGTGTAC CAGCTCAGCG CCAACGGGAC AGTGCAGACA
    CCCGGCACCT CTCGGAAGTC CCTGGACGCC CCCTCTTGGC CTGGCGTCTA CCGTCTCTCC
    ATGTCCTTGA TGGAGCGGCT CCTCAAAACC CTGCGCTACA ACTTCCTGAC CGAGGCCCTG
    GACTTCGTGG GGGTTCACCA GGAGCGGACC TTACAGTGCC TCAATGCCGT GAGGACGGTG
    CAGAGTCTGG CGTGCCTGGA GGAGGCTGAC CACACCGTGG GCTTCCTCCT ACAGCTCTCC
    AGCTTCAGGA AGGAGTGGCA CTTCCACCTG CCTCAGCTCA TGCGGGATGT CCAGGTGAAC
    CTCGGCTACC TGTGCCAGGC CTGCACCTCC CTCCTGCACA GCCGCAAGAT GCTGCAGCAC
    TACCTGCAGA ACAAAAATGG GGAGGGCATC CCCTCAGCTG TCGCCCCCCG AGCTCAGCGG
    CCACCCACAG CTGCCCCGGC TGCCCCCTCC TGCTCCTCCT CCAAGCAGCC TGCTCCCGAC
    ACGGAGGCCT CGGAACAGCG GGCCTTGCGC ACCGTCCAGT ATGGCCTCCT GAGGATCCTC
    AGCAGGACCC TGGCCGCCCT GCGCCGCTTC ACCCCCGACG TCTGCCAGGT CCTGCTGGAT
    CAGTCCCTGG ACCTTGCTGA GTACAGCTTC CTGTTTGCCC TGAGCTTCAC CACTCCCACT
    TTTGACTCTG AAGGGGCCCC CTCCTTCGGG ACCCTTCTGG CCACCGTGAA TGTGGCCCTG
    AACATGCTTG GAGAGCTGGA CAAGAAAAAG GAGCCTCTCA CCCAGGCTGT GGGGCTCAGC
    ACACAAGCAG AAGGGACCAG AACTTTGAAG TCGCTCCTGA TGTTCACCAT GGAGAACTGC
    TTCTACCTGC TCATCTCCCA GGCGATGCGT TACCTTCGGG ACCCGGCTGT GCACCCCCGG
    GACAAGCAGC GGATGAAGCA GGAGCTCAGC TCAGAGTTGA GCACTCTCCT CTCCAGCCTC
    TCCCGCTACT TCCGCCGGGG AGCCCCCAGC TCCCCTGCTG CTGGCGTCCT TCCCTCGCCT
    CAGGGCAAGT CCACCTCTCT CTCCAAGGTC AGCCCTGAGA GTCAGGAGCC TCTGATCCAG
    CTGGTGCAGG CCTTTGTCCG ACACGTGCAA AGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025117589.1
    CDS49..5304
    Translation

    Target ORF information:

    RefSeq Version XM_025261804.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nucleoporin 188 (NUP188), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025261804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCGGCTG CCGCCGGCGG TCCGTGTGTG AGGAGCAGTA GAGAACTGTG GACAATTCTG 
    CTAGGAAGGT CAGCTCTTAG AGAACTGAAT CAGATTGAGG CAGAGCTGAA TAAACATTGG
    CAGCGGCTGT TAGAAGGACT TTCTTACTAT AAACCTCCCA GTCCAAGTTC AGCTGAAAAA
    GTGAAAGCTA GTAAAGATGT AGCTTCACCA CTGAAGGAAC TGGGTTTAAG AATCAGCAAG
    TTTTTGGGTC TTGATGAAGA ACAGAGTGTG CAGTTACTCC AGTGTTACCT TCAAGAGGAT
    TACAGAGGTA CTCGGGACTC ACTAAAGACG GTGCTGCAAG ATGAGAGGCA GAGCCAGGCC
    TTAATCCTGA AGATTGCAGA TTATTACTAT GAAGAAAGAA CTTGTATTCT TCGTTGCGTC
    TTACACCTTC TCACTTATTT CCAAGATGAA AGACACCCCT ATAGGGTTGA ATATGCCGAC
    TGTGTGGATA AATTGGAAAA GGAGCTAGTT ACAAAATATA GACAGCAGTT TGAAGAGCTT
    TATAAAACTG AAGCACCAAC GTGGGAGACA CATGGAAACC TCATGACTGA GCGCCAGGTG
    TCTCGCTGGT TTGTTCAGTG CCTTCGGGAG CAGTCCATGC TGCTAGAGAT TATTTTCCTG
    TATTATGCAT ACTTTGAGAT GGCACCTAGT GACCTATTGG TGTTAACCAA GATGTTTAAA
    GAGCAAGGAT TTGGTAGTAG GCAGACTAAC AGGCACCTGG TAGATGAGAC CATGGATCCT
    TTTGTGGATC GGATTGGCTA CTTCAGTGCC CTTATTCTGG TGGAAGGCAT GGACATTGAA
    TCCTTGCATA AGTGTGCTTT GGATGACAGA AGAGAACTGC ACCAGTTTGC CCAGGATGGA
    CTTATTTGTC AGGATATGGA CCGTTTGATG TTGACCTTTG GGGACATTCC GCATCATGCC
    CCAGTGCTTT TGGCATGGGC CCTCCTCCGT CACACCCTGA ATCCAGAAGA GACGAGCAGT
    GTGGTCCGGA AGATAGGGGG GACAGCCATC CAACTGAACG TCTTTCAGTA CTTGACCCGA
    CTGCTCCGCT CGCTGGCCAG TGGGGGAAAT GACTGCACCA CCAGCACTGC CTGCATGTGT
    GTCTACGGAC TCCTTTCTTT TGTCCTGACC TCCCTGGAGC TGCACACCCT GGGCAATCAG
    CAGGATGTAA TTGACACAGC ATGTGAAGTC CTGGCAGACC CTTCTCTCCC AGAACTCTTC
    TGGGGAACAG AACCAACCTC TGGTCTCGGG ATCATTCTGG ACAGTGTATG TGGAATGTTT
    CCCCATCTCC TCTCTCCACT CCTGCAGTTG CTTCGAGCCC TTGTATCAGG GAAGTCTACT
    GCTAAGAAGG TGTATAGTTT CTTGGATAAG ATGTCTTTCT ACAATGAACT CTATAAGCAC
    AAGCCCCATG ATGTGATCTC CCATGAAGAT GGAACCCTTT GGCGGAGGCA AACACCCAAA
    CTCCTTTACC CTCTAGGGGG TCAGACCAAC CTTCGCATAC CTCAAGGCAC CGTGGGCCAA
    GTGATGCTGG ACGATAGGGC CTACCTGGTG CGCTGGGAAT ACTCGTACAG CAGCTGGACC
    CTCTTCACCT GCGAGATTGA GATGCTGCTG CATGTCGTTT CCACTGCAGA TGTGATTCAG
    CACTGCCAGC GGGTCAAACC CATCATCGAC CTGGTCCATA AGGTCATCAG CACAGACCTG
    TCGATAGCAG ACTGTCTCCT GCCCATCACG TCCCGCATCT ACATGCTACT GCAGCGGTTA
    ACAACAGTCA TCTCCCCGCC TGTGGATGTC ATTGCTTCTT GCGTCAATTG CTTGACTGTT
    TTGGCTGCCC GGAACCCAGC AAAGGTCTGG ACTGATCTTC TTCACACAGG CTTCTTGCCT
    TTCGTGGCCC ACCAGGTCTC CAGCATGAGT CAGATGATCA GTGCTGAGGG GATGAATGCT
    GGTGGGTATG GAAACCTCTT GATGAATAGT GAACAGCCCC AGGGCGAGTA TGGGGTCACT
    GTTGCCTTTC TGCGCTTGAT CACCACTCTT GTGAAGGGGC AGCTTGGTAG TACCCAGAGC
    CAAGGGCTGG TGCCTTGTGT CATGTTTGTG CTGAAGGAGA TGCTGCCAAG TTACCACAAG
    TGGCGCTACA ACTCCCATGG TGTGAGAGAA CAGATTGGTT GCCTGATCCT GGAGCTGATT
    CATTCAATAC TGAACCTGTG CCATGAGACA GACCTGCATA GCAGTCACAC CCCCAGCCTG
    CAGTCTCTCT GCATCTGCAG CCTGGCCTAC ACAGAAGCAG GACAGACAGT AATCAATATC
    ATGGGCATCG GTGTGGACAC CATCGACATG GTCATGGCTG CTCAGCCCCG CAGCGATGGG
    GCAGAGGGCC AGGGCCAGGG CCAGCTGCTG ATCAAGACAG TGAAATTGGC GTTCTCCGTC
    ACCAACAATG TCATTCGGCT GAAACCGCCT TCTAATGTGG TGTCCCCTCT GGAACAGGCT
    CTCACACAGC ATGGTGCCCA TGGGAACAAC CTTATTGCTG TTCTTGCCAA ATACATCTAC
    CACAAACACG ACCCAGCTCT GCCTCGTCTT GCCATCCAGC TGCTGAAACG TCTGGCCACG
    GTGGCCCCCA TGTCAGTGTA TGCCTGCCTG GGCAGTGATG CGGCTGCCAT CCGAGACGCC
    TTCCTGACCC GACTGCAGAG CAAGATTGAG GATATGCGCA TCAAAGTCAT GATTCTGGAA
    TTCCTCACGG TTGCTGTAGA GACGCAGCCA GGCCTCATTG AGCTGTTTCT GAATCTGGAG
    GTGAAGGATG GCAGCGATGG CTCCAAGGAA TTCAGCCTGG GGGTGTGGAG CTGTCTCCAC
    GCGGTGCTGG AGCTGATCGG TTCCCAGCAG CAGGACCGCT ACTGGTGCCC CCCACTGCTG
    CACCGGGCAG CCATCGCCTT TTTGCACGCC CTGTGGCAGG ACCGGCGGGA CAGTGCCATG
    CTGGTCCTCA GAACCAAACC CAAGTTTTGG GAGAATCTAA CGAGTCCGCT ATTTGGTACT
    CTTTCTCCTC CCTCGGAAAC ATCTGAGCCC AGCATCCTGG AAACCTGTGC CCTAATCATG
    AAGATAATTT GCTTGGAGAT ATACTATGTA GTTAAGGGCT CATTAGACCA ATCATTAAAA
    GATACACTCA AGAAATTTTC CAGCGAGAAG CGCTTTGCCT ACTGGTCGGG GTATGTCAAG
    TCCTTGGCGG TTCACATGGC CGACACAGAG GGCAGCAGCT GCACCTCCCT GGTAGAGTAT
    CAGATGCTGG TCTCTGCCTG GAGGATGTTT CTCATCATTG CCACCAGTCA CGCGGAGATA
    ACGCACCTGA CAGACTCTGC AGTGCGTCGC CAGCTCTTTC TTGACGTGCT CGAGGGGACC
    AAGGCGTTAC TCCTCGTCCC TACCTCCGTG AACTGCCTCC GCCTCGGCTC CATGACGTGC
    ACCCTGCTGC TTATTCTCCT CCGGCAGTGG AAAAGAGAGT TAGGGTCTGT GGATGAAATC
    CTCGGGCCCC TGACGGAGAT CCTGGAGGGA GTGCTGCAGG CAGACCAGCA GCTTATGGAG
    AAGACCAAGG CCAAGGTGTT CTCAGCGTTC ATCACGGTGC TGCAGATGAA GGAGATGAGC
    GTGAATGACA TCCCCCAGTA CTCCCAGCTG GTGCTGAACG TCTGTGAGAC CCTTCAGGAA
    GAGGTGATCG CACTTTTCGA CCACACCCGC CACAGCCTGG CGGCGGGCAG TGCCACCGAG
    GACAAGGACA GCATGGAGAC CGACGACTGC TCCCGGCTCC GGCACAAGGA CCAGCGGGAC
    GGGGTCTGTG TCCTGGGCCT GCACCTGGCC AAGGAGCTGT GTGAGGTGGA TGAGGACGGG
    GACTCGTGGC TGCAGGTGAC CCGCCGGCTC CCCATCCTGC CGACCCTCTT CACCACCTTG
    GAGGTGAGCC TGCGCGTGAA GCAGAACCTG CACTTCACCG AGGCGGCGCT GCACCTGCTC
    CTCACGCTGG CCCGCACCCA GCAGGGGGCC ACAGCCGTGG CCGGGGCCGG CGTCACCCAG
    AGCATTTGCT TGCCTCTCCT GAGTGTGTAC CAGCTCAGCG CCAACGGGAC AGTGCAGACA
    CCCGGCACCT CTCGGAAGTC CCTGGACGCC CCCTCTTGGC CTGGCGTCTA CCGTCTCTCC
    ATGTCCTTGA TGGAGCGGCT CCTCAAAACC CTGCGCTACA ACTTCCTGAC CGAGGCCCTG
    GACTTCGTGG GGGTTCACCA GGAGCGGACC TTACAGTGCC TCAATGCCGT GAGGACGGTG
    CAGAGTCTGG CGTGCCTGGA GGAGGCTGAC CACACCGTGG GCTTCCTCCT ACAGCTCTCC
    AGCTTCAGGA AGGAGTGGCA CTTCCACCTG CCTCAGCTCA TGCGGGATGT CCAGGTGAAC
    CTCGGCTACC TGTGCCAGGC CTGCACCTCC CTCCTGCACA GCCGCAAGAT GCTGCAGCAC
    TACCTGCAGA ACAAAAATGG GGAGGGCATC CCCTCAGCTG TCGCCCCCCG AGCTCAGCGG
    CCACCCACAG CTGCCCCGGC TGCCCCCTCC TGCTCCTCCT CCAAGCAGCC TGCTCCCGAC
    ACGGAGGCCT CGGAACAGCG GGCCTTGCGC ACCGTCCAGT ATGGCCTCCT GAGGATCCTC
    AGCAGGACCC TGGCCGCCCT GCGCCGCTTC ACCCCCGACG TCTGCCAGGT CCTGCTGGAT
    CAGTCCCTGG ACCTTGCTGA GTACAGCTTC CTGTTTGCCC TGAGCTTCAC CACTCCCACT
    TTTGACTCTG AAGGGGCCCC CTCCTTCGGG ACCCTTCTGG CCACCGTGAA TGTGGCCCTG
    AACATGCTTG GAGAGCTGGA CAAGAAAAAG GAGCCTCTCA CCCAGGCTGT GGGGCTCAGC
    ACACAAGCAG AAGGGACCAG AACTTTGAAG TCGCTCCTGA TGTTCACCAT GGAGAACTGC
    TTCTACCTGC TCATCTCCCA GGCGATGCGT TACCTTCGGG ACCCGGCTGT GCACCCCCGG
    GACAAGCAGC GGATGAAGCA GGAGCTCAGC TCAGAGTTGA GCACTCTCCT CTCCAGCCTC
    TCCCGCTACT TCCGCCGGGG AGCCCCCAGC TCCCCTGCTG CTGGCGTCCT TCCCTCGCCT
    CAGGGCAAGT CCACCTCTCT CTCCAAGGTC AGCCCTGAGA GTCAGGAGCC TCTGATCCAG
    CTGGTGCAGG CCTTTGTCCG ACACGTGCAA AGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu46692
    Clone ID Related Accession (Same CDS sequence) XM_025261805.1
    Accession Version XM_025261805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5196bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product nucleoporin NUP188 homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGGCTG CCGCCGGCGG TCCGTGTGTG AGGAGCAGTA GAGAACTGTG GACAATTCTG 
    CTAGGAAGGT CAGCTCTTAG AGAACTGAAT CAGATTGAGG CAGAGCTGAA TAAACATTGG
    CAGCGGCTGT TAGAAGGACT TTCTTACTAT AAACCTCCCA GTCCAAGTTC AGCTGAAAAA
    GTGAAAGCTA GTAAAGATGT AGCTTCACCA CTGAAGGAAC TGGGTTTAAG AATCAGCAAG
    TTTTTGGGTC TTGATGAAGA ACAGAGTGTG CAGTTACTCC AGTGTTACCT TCAAGAGGAT
    TACAGAGGTA CTCGGGACTC ACTAAAGACG GTGCTGCAAG ATGAGAGGCA GAGCCAGGCC
    TTAATCCTGA AGATTGCAGA TTATTACTAT GAAGAAAGAA CTTGTATTCT TCGTTGCGTC
    TTACACCTTC TCACTTATTT CCAAGATGAA AGACACCCCT ATAGGGTTGA ATATGCCGAC
    TGTGTGGATA AATTGGAAAA GGAGCTAGTT ACAAAATATA GACAGCAGTT TGAAGAGCTT
    TATAAAACTG AAGCACCAAC GTGGGAGACA CATGGAAACC TCATGACTGA GCGCCAGGTG
    TCTCGCTGGT TTGTTCAGTG CCTTCGGGAG CAGTCCATGC TGCTAGAGAT TATTTTCCTG
    TATTATGCAT ACTTTGAGAT GGCACCTAGT GACCTATTGG TGTTAACCAA GATGTTTAAA
    GAGCAAGGAT TTGGTAGTAG GCAGACTAAC AGGCACCTGG TAGATGAGAC CATGGATCCT
    TTTGTGGATC GGATTGGCTA CTTCAGTGCC CTTATTCTGG TGGAAGGCAT GGACATTGAA
    TCCTTGCATA AGTGTGCTTT GGATGACAGA AGAGAACTGC ACCAGTTTGC CCAGGATGGA
    CTTATTTGTC AGGATATGGA CCGTTTGATG TTGACCTTTG GGGACATTCC GCATCATGCC
    CCAGTGCTTT TGGCATGGGC CCTCCTCCGT CACACCCTGA ATCCAGAAGA GACGAGCAGT
    GTGGTCCGGA AGATAGGGGG GACAGCCATC CAACTGAACG TCTTTCAGTA CTTGACCCGA
    CTGCTCCGCT CGCTGGCCAG TGGGGGAAAT GACTGCACCA CCAGCACTGC CTGCATGTGT
    GTCTACGGAC TCCTTTCTTT TGTCCTGACC TCCCTGGAGC TGCACACCCT GGGCAATCAG
    CAGGATGTAA TTGACACAGC ATGTGAAGTC CTGGCAGACC CTTCTCTCCC AGAACTCTTC
    TGGGGAACAG AACCAACCTC TGGTCTCGGG ATCATTCTGG ACAGTGTATG TGGAATGTTT
    CCCCATCTCC TCTCTCCACT CCTGCAGTTG CTTCGAGCCC TTGTATCAGG GAAGTCTACT
    GCTAAGAAGG TGTATAGTTT CTTGGATAAG ATGTCTTTCT ACAATGAACT CTATAAGCAC
    AAGCCCCATG ATGTGATCTC CCATGAAGAT GGAACCCTTT GGCGGAGGCA AACACCCAAA
    CTCCTTTACC CTCTAGGGGG TCAGACCAAC CTTCGCATAC CTCAAGGCAC CGTGGGCCAA
    GTGATGCTGG ACGATAGGGC CTACCTGGTG CGCTGGGAAT ACTCGTACAG CAGCTGGACC
    CTCTTCACCT GCGAGATTGA GATGCTGCTG CATGTCGTTT CCACTGCAGA TGTGATTCAG
    CACTGCCAGC GGGTCAAACC CATCATCGAC CTGGTCCATA AGGTCATCAG CACAGACCTG
    TCGATAGCAG ACTGTCTCCT GCCCATCACG TCCCGCATCT ACATGCTACT GCAGCGGTTA
    ACAACAGTCA TCTCCCCGCC TGTGGATGTC ATTGCTTCTT GCGTCAATTG CTTGACTGTT
    TTGGCTGCCC GGAACCCAGC AAAGGTCTGG ACTGATCTTC TTCACACAGG CTTCTTGCCT
    TTCGTGGCCC ACCAGGTCTC CAGCATGAGT CAGATGATCA GTGCTGAGGG GATGAATGCT
    GGTGGGTATG GAAACCTCTT GATGAATAGT GAACAGCCCC AGGGCGAGTA TGGGGTCACT
    GTTGCCTTTC TGCGCTTGAT CACCACTCTT GTGAAGGGGC AGCTTGGTAG TACCCAGAGC
    CAAGGGCTGG TGCCTTGTGT CATGTTTGTG CTGAAGGAGA TGCTGCCAAG TTACCACAAG
    TGGCGCTACA ACTCCCATGG TGTGAGAGAA CAGATTGGTT GCCTGATCCT GGAGCTGATT
    CATTCAATAC TGAACCTGTG CCATGAGACA GACCTGCATA GCAGTCACAC CCCCAGCCTG
    CAGTCTCTCT GCATCTGCAG CCTGGCCTAC ACAGAAGCAG GACAGACAGT AATCAATATC
    ATGGGCATCG GTGTGGACAC CATCGACATG GTCATGGCTG CTCAGCCCCG CAGCGATGGG
    GCAGAGGGCC AGGGCCAGGG CCAGCTGCTG ATCAAGACAG TGAAATTGGC GTTCTCCGTC
    ACCAACAATG TCATTCGGCT GAAACCGCCT TCTAATGTGG TGTCCCCTCT GGAACAGGCT
    CTCACACAGC ATGGTGCCCA TGGGAACAAC CTTATTGCTG TTCTTGCCAA ATACATCTAC
    CACAAACACG ACCCAGCTCT GCCTCGTCTT GCCATCCAGC TGCTGAAACG TCTGGCCACG
    GTGGCCCCCA TGTCAGTGTA TGCCTGCCTG GGCAGTGATG CGGCTGCCAT CCGAGACGCC
    TTCCTGACCC GACTGCAGAG CAAGATTGAG GATATGCGCA TCAAAGTCAT GATTCTGGAA
    TTCCTCACGG TTGCTGTAGA GACGCAGCCA GGCCTCATTG AGCTGTTTCT GAATCTGGAG
    GTGAAGGATG GCAGCGATGG CTCCAAGGAA TTCAGCCTGG GGGTGTGGAG CTGTCTCCAC
    GCGGTGCTGG AGCTGATCGG TTCCCAGCAG CAGGACCGCT ACTGGTGCCC CCCACTGCTG
    CACCGGGCAG CCATCGCCTT TTTGCACGCC CTGTGGCAGG ACCGGCGGGA CAGTGCCATG
    CTGGTCCTCA GAACCAAACC CAAGTTTTGG GAGAATCTAA CGAGTCCGCT ATTTGGTACT
    CTTTCTCCTC CCTCGGAAAC ATCTGAGCCC AGCATCCTGG AAACCTGTGC CCTAATCATG
    AAGATAATTT GCTTGGAGAT ATACTATGTA GTTAAGGGCT CATTAGACCA ATCATTAAAA
    GATACACTCA AGAAATTTTC CAGCGAGAAG CGCTTTGCCT ACTGGTCGGG GTATGTCAAG
    TCCTTGGCGG TTCACATGGC CGACACAGAG GGCAGCAGCT GCACCTCCCT GGTAGAGTAT
    CAGATGCTGG TCTCTGCCTG GAGGATGTTT CTCATCATTG CCACCAGTCA CGCGGAGATA
    ACGCACCTGA CAGACTCTGC AGTGCGTCGC CAGCTCTTTC TTGACGTGCT CGAGGGGACC
    AAGGCGTTAC TCCTCGTCCC TACCTCCGTG AACTGCCTCC GCCTCGGCTC CATGACGTGC
    ACCCTGCTGC TTATTCTCCT CCGGCAGTGG AAAAGAGAGT TAGGGTCTGT GGATGAAATC
    CTCGGGCCCC TGACGGAGAT CCTGGAGGGA GTGCTGCAGG CAGACCAGCA GCTTATGGAG
    AAGACCAAGG CCAAGGTGTT CTCAGCGTTC ATCACGGTGC TGCAGATGAA GGAGATGAGC
    GTGAATGACA TCCCCCAGTA CTCCCAGCTG GTGCTGAACG TCTGTGAGAC CCTTCAGGAA
    GAGGTGATCG CACTTTTCGA CCACACCCGC CACAGCCTGG CGGCGGGCAG TGCCACCGAG
    GACAAGGACA GCATGGAGAC CGACGACTGC TCCCGGCTCC GGCACAAGGA CCAGCGGGAC
    GGGGTCTGTG TCCTGGGCCT GCACCTGGCC AAGGAGCTGT GTGAGGTGGA TGAGGACGGG
    GACTCGTGGC TGCAGGTGAC CCGCCGGCTC CCCATCCTGC CGACCCTCTT CACCACCTTG
    GAGGTGAGCC TGCGCGTGAA GCAGAACCTG CACTTCACCG AGGCGGCGCT GCACCTGCTC
    CTCACGCTGG CCCGCACCCA GCAGGGGGCC ACAGCCGTGG CCGGGGCCGG CGTCACCCAG
    AGCATTTGCT TGCCTCTCCT GAGTGTGTAC CAGCTCAGCG CCAACGGGAC AGTGCAGACA
    CCCGGCACCT CTCGGAAGTC CCTGGACGCC CCCTCTTGGC CTGGCGTCTA CCGTCTCTCC
    ATGTCCTTGA TGGAGCGGCT CCTCAAAACC CTGCGCTACA ACTTCCTGAC CGAGGCCCTG
    GACTTCGTGG GGGTTCACCA GGAGCGGACC TTACAGCTCT CCAGCTTCAG GAAGGAGTGG
    CACTTCCACC TGCCTCAGCT CATGCGGGAT GTCCAGGTGA ACCTCGGCTA CCTGTGCCAG
    GCCTGCACCT CCCTCCTGCA CAGCCGCAAG ATGCTGCAGC ACTACCTGCA GAACAAAAAT
    GGGGAGGGCA TCCCCTCAGC TGTCGCCCCC CGAGCTCAGC GGCCACCCAC AGCTGCCCCG
    GCTGCCCCCT CCTGCTCCTC CTCCAAGCAG CCTGCTCCCG ACACGGAGGC CTCGGAACAG
    CGGGCCTTGC GCACCGTCCA GTATGGCCTC CTGAGGATCC TCAGCAGGAC CCTGGCCGCC
    CTGCGCCGCT TCACCCCCGA CGTCTGCCAG GTCCTGCTGG ATCAGTCTCA TCTTGCTTTG
    CAGTCCCTGG ACCTTGCTGA GTACAGCTTC CTGTTTGCCC TGAGCTTCAC CACTCCCACT
    TTTGACTCTG AAGGGGCCCC CTCCTTCGGG ACCCTTCTGG CCACCGTGAA TGTGGCCCTG
    AACATGCTTG GAGAGCTGGA CAAGAAAAAG GAGCCTCTCA CCCAGGCTGT GGGGCTCAGC
    ACACAAGCAG AAGGGACCAG AACTTTGAAG TCGCTCCTGA TGTTCACCAT GGAGAACTGC
    TTCTACCTGC TCATCTCCCA GGCGATGCGT TACCTTCGGG ACCCGGCTGT GCACCCCCGG
    GACAAGCAGC GGATGAAGCA GGAGCTCAGC TCAGAGTTGA GCACTCTCCT CTCCAGCCTC
    TCCCGCTACT TCCGCCGGGG AGCCCCCAGC TCCCCTGCTG CTGGCGTCCT TCCCTCGCCT
    CAGGGCAAGT CCACCTCTCT CTCCAAGGTC AGCCCTGAGA GTCAGGAGCC TCTGATCCAG
    CTGGTGCAGG CCTTTGTCCG ACACGTGCAA AGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025117590.1
    CDS49..5244
    Translation

    Target ORF information:

    RefSeq Version XM_025261805.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nucleoporin 188 (NUP188), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025261805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCGGCTG CCGCCGGCGG TCCGTGTGTG AGGAGCAGTA GAGAACTGTG GACAATTCTG 
    CTAGGAAGGT CAGCTCTTAG AGAACTGAAT CAGATTGAGG CAGAGCTGAA TAAACATTGG
    CAGCGGCTGT TAGAAGGACT TTCTTACTAT AAACCTCCCA GTCCAAGTTC AGCTGAAAAA
    GTGAAAGCTA GTAAAGATGT AGCTTCACCA CTGAAGGAAC TGGGTTTAAG AATCAGCAAG
    TTTTTGGGTC TTGATGAAGA ACAGAGTGTG CAGTTACTCC AGTGTTACCT TCAAGAGGAT
    TACAGAGGTA CTCGGGACTC ACTAAAGACG GTGCTGCAAG ATGAGAGGCA GAGCCAGGCC
    TTAATCCTGA AGATTGCAGA TTATTACTAT GAAGAAAGAA CTTGTATTCT TCGTTGCGTC
    TTACACCTTC TCACTTATTT CCAAGATGAA AGACACCCCT ATAGGGTTGA ATATGCCGAC
    TGTGTGGATA AATTGGAAAA GGAGCTAGTT ACAAAATATA GACAGCAGTT TGAAGAGCTT
    TATAAAACTG AAGCACCAAC GTGGGAGACA CATGGAAACC TCATGACTGA GCGCCAGGTG
    TCTCGCTGGT TTGTTCAGTG CCTTCGGGAG CAGTCCATGC TGCTAGAGAT TATTTTCCTG
    TATTATGCAT ACTTTGAGAT GGCACCTAGT GACCTATTGG TGTTAACCAA GATGTTTAAA
    GAGCAAGGAT TTGGTAGTAG GCAGACTAAC AGGCACCTGG TAGATGAGAC CATGGATCCT
    TTTGTGGATC GGATTGGCTA CTTCAGTGCC CTTATTCTGG TGGAAGGCAT GGACATTGAA
    TCCTTGCATA AGTGTGCTTT GGATGACAGA AGAGAACTGC ACCAGTTTGC CCAGGATGGA
    CTTATTTGTC AGGATATGGA CCGTTTGATG TTGACCTTTG GGGACATTCC GCATCATGCC
    CCAGTGCTTT TGGCATGGGC CCTCCTCCGT CACACCCTGA ATCCAGAAGA GACGAGCAGT
    GTGGTCCGGA AGATAGGGGG GACAGCCATC CAACTGAACG TCTTTCAGTA CTTGACCCGA
    CTGCTCCGCT CGCTGGCCAG TGGGGGAAAT GACTGCACCA CCAGCACTGC CTGCATGTGT
    GTCTACGGAC TCCTTTCTTT TGTCCTGACC TCCCTGGAGC TGCACACCCT GGGCAATCAG
    CAGGATGTAA TTGACACAGC ATGTGAAGTC CTGGCAGACC CTTCTCTCCC AGAACTCTTC
    TGGGGAACAG AACCAACCTC TGGTCTCGGG ATCATTCTGG ACAGTGTATG TGGAATGTTT
    CCCCATCTCC TCTCTCCACT CCTGCAGTTG CTTCGAGCCC TTGTATCAGG GAAGTCTACT
    GCTAAGAAGG TGTATAGTTT CTTGGATAAG ATGTCTTTCT ACAATGAACT CTATAAGCAC
    AAGCCCCATG ATGTGATCTC CCATGAAGAT GGAACCCTTT GGCGGAGGCA AACACCCAAA
    CTCCTTTACC CTCTAGGGGG TCAGACCAAC CTTCGCATAC CTCAAGGCAC CGTGGGCCAA
    GTGATGCTGG ACGATAGGGC CTACCTGGTG CGCTGGGAAT ACTCGTACAG CAGCTGGACC
    CTCTTCACCT GCGAGATTGA GATGCTGCTG CATGTCGTTT CCACTGCAGA TGTGATTCAG
    CACTGCCAGC GGGTCAAACC CATCATCGAC CTGGTCCATA AGGTCATCAG CACAGACCTG
    TCGATAGCAG ACTGTCTCCT GCCCATCACG TCCCGCATCT ACATGCTACT GCAGCGGTTA
    ACAACAGTCA TCTCCCCGCC TGTGGATGTC ATTGCTTCTT GCGTCAATTG CTTGACTGTT
    TTGGCTGCCC GGAACCCAGC AAAGGTCTGG ACTGATCTTC TTCACACAGG CTTCTTGCCT
    TTCGTGGCCC ACCAGGTCTC CAGCATGAGT CAGATGATCA GTGCTGAGGG GATGAATGCT
    GGTGGGTATG GAAACCTCTT GATGAATAGT GAACAGCCCC AGGGCGAGTA TGGGGTCACT
    GTTGCCTTTC TGCGCTTGAT CACCACTCTT GTGAAGGGGC AGCTTGGTAG TACCCAGAGC
    CAAGGGCTGG TGCCTTGTGT CATGTTTGTG CTGAAGGAGA TGCTGCCAAG TTACCACAAG
    TGGCGCTACA ACTCCCATGG TGTGAGAGAA CAGATTGGTT GCCTGATCCT GGAGCTGATT
    CATTCAATAC TGAACCTGTG CCATGAGACA GACCTGCATA GCAGTCACAC CCCCAGCCTG
    CAGTCTCTCT GCATCTGCAG CCTGGCCTAC ACAGAAGCAG GACAGACAGT AATCAATATC
    ATGGGCATCG GTGTGGACAC CATCGACATG GTCATGGCTG CTCAGCCCCG CAGCGATGGG
    GCAGAGGGCC AGGGCCAGGG CCAGCTGCTG ATCAAGACAG TGAAATTGGC GTTCTCCGTC
    ACCAACAATG TCATTCGGCT GAAACCGCCT TCTAATGTGG TGTCCCCTCT GGAACAGGCT
    CTCACACAGC ATGGTGCCCA TGGGAACAAC CTTATTGCTG TTCTTGCCAA ATACATCTAC
    CACAAACACG ACCCAGCTCT GCCTCGTCTT GCCATCCAGC TGCTGAAACG TCTGGCCACG
    GTGGCCCCCA TGTCAGTGTA TGCCTGCCTG GGCAGTGATG CGGCTGCCAT CCGAGACGCC
    TTCCTGACCC GACTGCAGAG CAAGATTGAG GATATGCGCA TCAAAGTCAT GATTCTGGAA
    TTCCTCACGG TTGCTGTAGA GACGCAGCCA GGCCTCATTG AGCTGTTTCT GAATCTGGAG
    GTGAAGGATG GCAGCGATGG CTCCAAGGAA TTCAGCCTGG GGGTGTGGAG CTGTCTCCAC
    GCGGTGCTGG AGCTGATCGG TTCCCAGCAG CAGGACCGCT ACTGGTGCCC CCCACTGCTG
    CACCGGGCAG CCATCGCCTT TTTGCACGCC CTGTGGCAGG ACCGGCGGGA CAGTGCCATG
    CTGGTCCTCA GAACCAAACC CAAGTTTTGG GAGAATCTAA CGAGTCCGCT ATTTGGTACT
    CTTTCTCCTC CCTCGGAAAC ATCTGAGCCC AGCATCCTGG AAACCTGTGC CCTAATCATG
    AAGATAATTT GCTTGGAGAT ATACTATGTA GTTAAGGGCT CATTAGACCA ATCATTAAAA
    GATACACTCA AGAAATTTTC CAGCGAGAAG CGCTTTGCCT ACTGGTCGGG GTATGTCAAG
    TCCTTGGCGG TTCACATGGC CGACACAGAG GGCAGCAGCT GCACCTCCCT GGTAGAGTAT
    CAGATGCTGG TCTCTGCCTG GAGGATGTTT CTCATCATTG CCACCAGTCA CGCGGAGATA
    ACGCACCTGA CAGACTCTGC AGTGCGTCGC CAGCTCTTTC TTGACGTGCT CGAGGGGACC
    AAGGCGTTAC TCCTCGTCCC TACCTCCGTG AACTGCCTCC GCCTCGGCTC CATGACGTGC
    ACCCTGCTGC TTATTCTCCT CCGGCAGTGG AAAAGAGAGT TAGGGTCTGT GGATGAAATC
    CTCGGGCCCC TGACGGAGAT CCTGGAGGGA GTGCTGCAGG CAGACCAGCA GCTTATGGAG
    AAGACCAAGG CCAAGGTGTT CTCAGCGTTC ATCACGGTGC TGCAGATGAA GGAGATGAGC
    GTGAATGACA TCCCCCAGTA CTCCCAGCTG GTGCTGAACG TCTGTGAGAC CCTTCAGGAA
    GAGGTGATCG CACTTTTCGA CCACACCCGC CACAGCCTGG CGGCGGGCAG TGCCACCGAG
    GACAAGGACA GCATGGAGAC CGACGACTGC TCCCGGCTCC GGCACAAGGA CCAGCGGGAC
    GGGGTCTGTG TCCTGGGCCT GCACCTGGCC AAGGAGCTGT GTGAGGTGGA TGAGGACGGG
    GACTCGTGGC TGCAGGTGAC CCGCCGGCTC CCCATCCTGC CGACCCTCTT CACCACCTTG
    GAGGTGAGCC TGCGCGTGAA GCAGAACCTG CACTTCACCG AGGCGGCGCT GCACCTGCTC
    CTCACGCTGG CCCGCACCCA GCAGGGGGCC ACAGCCGTGG CCGGGGCCGG CGTCACCCAG
    AGCATTTGCT TGCCTCTCCT GAGTGTGTAC CAGCTCAGCG CCAACGGGAC AGTGCAGACA
    CCCGGCACCT CTCGGAAGTC CCTGGACGCC CCCTCTTGGC CTGGCGTCTA CCGTCTCTCC
    ATGTCCTTGA TGGAGCGGCT CCTCAAAACC CTGCGCTACA ACTTCCTGAC CGAGGCCCTG
    GACTTCGTGG GGGTTCACCA GGAGCGGACC TTACAGCTCT CCAGCTTCAG GAAGGAGTGG
    CACTTCCACC TGCCTCAGCT CATGCGGGAT GTCCAGGTGA ACCTCGGCTA CCTGTGCCAG
    GCCTGCACCT CCCTCCTGCA CAGCCGCAAG ATGCTGCAGC ACTACCTGCA GAACAAAAAT
    GGGGAGGGCA TCCCCTCAGC TGTCGCCCCC CGAGCTCAGC GGCCACCCAC AGCTGCCCCG
    GCTGCCCCCT CCTGCTCCTC CTCCAAGCAG CCTGCTCCCG ACACGGAGGC CTCGGAACAG
    CGGGCCTTGC GCACCGTCCA GTATGGCCTC CTGAGGATCC TCAGCAGGAC CCTGGCCGCC
    CTGCGCCGCT TCACCCCCGA CGTCTGCCAG GTCCTGCTGG ATCAGTCTCA TCTTGCTTTG
    CAGTCCCTGG ACCTTGCTGA GTACAGCTTC CTGTTTGCCC TGAGCTTCAC CACTCCCACT
    TTTGACTCTG AAGGGGCCCC CTCCTTCGGG ACCCTTCTGG CCACCGTGAA TGTGGCCCTG
    AACATGCTTG GAGAGCTGGA CAAGAAAAAG GAGCCTCTCA CCCAGGCTGT GGGGCTCAGC
    ACACAAGCAG AAGGGACCAG AACTTTGAAG TCGCTCCTGA TGTTCACCAT GGAGAACTGC
    TTCTACCTGC TCATCTCCCA GGCGATGCGT TACCTTCGGG ACCCGGCTGT GCACCCCCGG
    GACAAGCAGC GGATGAAGCA GGAGCTCAGC TCAGAGTTGA GCACTCTCCT CTCCAGCCTC
    TCCCGCTACT TCCGCCGGGG AGCCCCCAGC TCCCCTGCTG CTGGCGTCCT TCCCTCGCCT
    CAGGGCAAGT CCACCTCTCT CTCCAAGGTC AGCCCTGAGA GTCAGGAGCC TCTGATCCAG
    CTGGTGCAGG CCTTTGTCCG ACACGTGCAA AGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu20882
    Clone ID Related Accession (Same CDS sequence) XM_006065324.1
    Accession Version XM_006065324.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product nucleoporin NUP188 homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785166.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTTGGCCC CGCCCCCGCA GCCACGTGAC GAAAACCCAG TTCACGCCCT GGAAGCGCGC 
    GGTCACGTGG GCGTGGCGTC TGGGGGCGGG GCTGGGGCGC GCGCGCGCGC GAAGATGGCG
    GCTGCCGCCG GCGGTCCGTG TGTGAGGAGC AGTAGAGAAC TGTGGACAAT TCTGCTAGGA
    AGGTCAGCTC TTAGAGAACT GAATCAGATT GAGGCAGAGC TGAATAAACA TTGGCAGCGG
    CTGTTAGAAG GACTTTCTTA CTATAAACCT CCCAGTCCAA GTTCAGCTGA AAAAGTGAAA
    GCTAGTAAAG ATGTAGCTTC ACCACTGAAG GAACTGGGTT TAAGAATCAG CAAGTTTTTG
    GGTCTTGATG AAGAACAGAG TGTGCAGTTA CTCCAGTGTT ACCTTCAAGA GGATTACAGA
    GGTACTCGGG ACTCACTAAA GACGGTGCTG CAAGATGAGA GGCAGAGCCA GGCCTTAATC
    CTGAAGATTG CAGATTATTA CTATGAAGAA AGAACTTGTA TTCTTCGTTG CGTCTTACAC
    CTTCTCACTT ATTTCCAAGA TGAAAGACAC CCCTATAGGG TTGAATATGC CGACTGTGTG
    GATAAATTGG AAAAGGAGCT AGTTACAAAA TATAGACAGC AGTTTGAAGA GCTTTATAAA
    ACTGAAGCAC CAACGTGGGA GACACATGGA AACCTCATGA CTGAGCGCCA GGTGTCTCGC
    TGGTTTGTTC AGTGCCTTCG GGAGCAGTCC ATGCTGCTAG AGATTATTTT CCTGTATTAT
    GCATACTTTG AGATGGCACC TAGTGACCTA TTGGTGTTAA CCAAGATGTT TAAAGAGCAA
    GGATTTGGTA GTAGGCAGAC TAACAGGCAC CTGGTAGATG AGACCATGGA TCCTTTTGTG
    GATCGGATTG GCTACTTCAG TGCCCTTATT CTGGTGGAAG GCATGGACAT TGAATCCTTG
    CATAAGTGTG CTTTGGATGA CAGAAGAGAA CTGCACCAGT TTGCCCAGGA TGGACTTATT
    TGTCAGGATA TGGACCGTTT GATGTTGACC TTTGGGGACA TTCCGCATCA TGCCCCAGTG
    CTTTTGGCAT GGGCCCTCCT CCGTCACACC CTGAATCCAG AAGAGACGAG CAGTGTGGTC
    CGGAAGATAG GGGGGACAGC CATCCAACTG AACGTCTTTC AGTACTTGAC CCGACTGCTC
    CGCTCGCTGG CCAGTGGGGG AAATGACTGC ACCACCAGCA CTGCCTGCAT GTGTGTCTAC
    GGACTCCTTT CTTTTGTCCT GACCTCCCTG GAGCTGCACA CCCTGGGCAA TCAGCAGGAT
    GTAATTGACA CAGCATGTGA AGTCCTGGCA GACCCTTCTC TCCCAGAACT CTTCTGGGGA
    ACAGAACCAA CCTCTGGTCT CGGGATCATT CTGGACAGTG TATGTGGAAT GTTTCCCCAT
    CTCCTCTCTC CACTCCTGCA GTTGCTTCGA GCCCTTGTAT CAGGGAAGTC TACTGCTAAG
    AAGGTGTATA GTTTCTTGGA TAAGATGTCT TTCTACAATG AACTCTATAA GCACAAGCCC
    CATGATGTGA TCTCCCATGA AGATGGAACC CTTTGGCGGA GGCAAACACC CAAACTCCTT
    TACCCTCTAG GGGGTCAGAC CAACCTTCGC ATACCTCAAG GCACCGTGGG CCAAGTGATG
    CTGGACGATA GGGCCTACCT GGTGCGCTGG GAATACTCGT ACAGCAGCTG GACCCTCTTC
    ACCTGCGAGA TTGAGATGCT GCTGCATGTC GTTTCCACTG CAGATGTGAT TCAGCACTGC
    CAGCGGGTCA AACCCATCAT CGACCTGGTC CATAAGGTCA TCAGCACAGA CCTGTCGATA
    GCAGACTGTC TCCTGCCCAT CACGTCCCGC ATCTACATGC TACTGCAGCG GTTAACAACA
    GTCATCTCCC CGCCTGTGGA TGTCATTGCT TCTTGCGTCA ATTGCTTGAC TGTTTTGGCT
    GCCCGGAACC CAGCAAAGGT CTGGACTGAT CTTCTTCACA CAGGCTTCTT GCCTTTCGTG
    GCCCACCAGG TCTCCAGCAT GAGTCAGATG ATCAGTGCTG AGGGGATGAA TGCTGGTGGG
    TATGGAAACC TCTTGATGAA TAGTGAACAG CCCCAGGGCG AGTATGGGGT CACTGTTGCC
    TTTCTGCGCT TGATCACCAC TCTTGTGAAG GGGCAGCTTG GTAGTACCCA GAGCCAAGGG
    CTGGTGCCTT GTGTCATGTT TGTGCTGAAG GAGATGCTGC CAAGTTACCA CAAGTGGCGC
    TACAACTCCC ATGGTGTGAG AGAACAGATT GGTTGCCTGA TCCTGGAGCT GATTCATTCA
    ATACTGAACC TGTGCCATGA GACAGACCTG CATAGCAGTC ACACCCCCAG CCTGCAGTCT
    CTCTGCATCT GCAGCCTGGC CTACACAGAA GCAGGACAGA CAGTAATCAA TATCATGGGC
    ATCGGTGTGG ACACCATCGA CATGGTCATG GCTGCTCAGC CCCGCAGCGA TGGGGCAGAG
    GGCCAGGGCC AGGGCCAGCT GCTGATCAAG ACAGTGAAAT TGGCGTTCTC CGTCACCAAC
    AATGTCATTC GGCTGAAACC GCCTTCTAAT GTGGTGTCCC CTCTGGAACA GGCTCTCACA
    CAGCATGGTG CCCATGGGAA CAACCTTATT GCTGTTCTTG CCAAATACAT CTACCACAAA
    CACGACCCAG CTCTGCCTCG TCTTGCCATC CAGCTGCTGA AACGTCTGGC CACGGTGGCC
    CCCATGTCAG TGTATGCCTG CCTGGGCAGT GATGCGGCTG CCATCCGAGA CGCCTTCCTG
    ACCCGACTGC AGAGCAAGAT TGAGGATATG CGCATCAAAG TCATGATTCT GGAATTCCTC
    ACGGTTGCTG TAGAGACGCA GCCAGGCCTC ATTGAGCTGT TTCTGAATCT GGAGGTGAAG
    GATGGCAGCG ATGGCTCCAA GGAATTCAGC CTGGGGGTGT GGAGCTGTCT CCACGCGGTG
    CTGGAGCTGA TCGGTTCCCA GCAGCAGGAC CGCTACTGGT GCCCCCCACT GCTGCACCGG
    GCAGCCATCG CCTTTTTGCA CGCCCTGTGG CAGGACCGGC GGGACAGTGC CATGCTGGTC
    CTCAGAACCA AACCCAAGTT TTGGGAGAAT CTAACGAGTC CGCTATTTGG TACTCTTTCT
    CCTCCCTCGG AAACATCTGA GCCCAGCATC CTGGAAACCT GTGCCCTAAT CATGAAGATA
    ATTTGCTTGG AGATATACTA TGTAGTTAAG GGCTCATTAG ACCAATCATT AAAAGATACA
    CTCAAGAAAT TTTCCAGCGA GAAGCGCTTT GCCTACTGGT CGGGGTATGT CAAGTCCTTG
    GCGGTTCACA TGGCCGACAC AGAGGGCAGC AGCTGCACCT CCCTGGTAGA GTATCAGATG
    CTGGTCTCTG CCTGGAGGAT GTTTCTCATC ATTGCCACCA GTCACGCGGA GATAACGCAC
    CTGACAGACT CTGCAGTGCG TCGCCAGCTC TTTCTTGACG TGCTCGAGGG GACCAAGGCG
    TTACTCCTCG TCCCTACCTC CGTGAACTGC CTCCGCCTCG GCTCCATGAC GTGCACCCTG
    CTGCTTATTC TCCTCCGGCA GTGGAAAAGA GAGTTAGGGT CTGTGGATGA AATCCTCGGG
    CCCCTGACGG AGATCCTGGA GGGAGTGCTG CAGGCAGACC AGCAGCTTAT GGAGAAGACC
    AAGGCCAAGG TGTTCTCAGC GTTCATCACG GTGCTGCAGA TGAAGGAGAT GAGCGTGAAT
    GACATCCCCC AGTACTCCCA GCTGGTGCTG AACGTCTGTG AGACCCTTCA GGAAGAGGTG
    ATCGCACTTT TCGACCACAC CCGCCACAGC CTGGCGGCGG GCAGTGCCAC CGAGGACAAG
    GACAGCATGG AGACCGACGA CTGCTCCCGG CTCCGGCACA AGGACCAGCG GGACGGGGTC
    TGTGTCCTGG GCCTGCACCT GGCCAAGGAG CTGTGTGAGG TGGATGAGGA CGGGGACTCG
    TGGCTGCAGG TGACCCGCCG GCTCCCCATC CTGCCGACCC TCTTCACCAC CTTGGAGGTG
    AGCCTGCGCG TGAAGCAGAA CCTGCACTTC ACCGAGGCGG CGCTGCACCT GCTCCTCACG
    CTGGCCCGCA CCCAGCAGGG GGCCACAGCC GTGGCCGGGG CCGGCGTCAC CCAGAGCATT
    TGCTTGCCTC TCCTGAGTGT GTACCAGCTC AGCGCCAACG GGACAGTGCA GACACCCGGC
    ACCTCTCGGA AGTCCCTGGA CGCCCCCTCT TGGCCTGGCG TCTACCGTCT CTCCATGTCC
    TTGATGGAGC GGCTCCTCAA AACCCTGCGC TACAACTTCC TGACCGAGGC CCTGGACTTC
    GTGGGGGTTC ACCAGGAGCG GACCTTACAG TGCCTCAATG CCGTGAGGAC GGTGCAGAGT
    CTGGCGTGCC TGGAGGAGGC TGACCACACC GTGGGCTTCC TCCTACAGCT CTCCAGCTTC
    AGGAAGGAGT GGCACTTCCA CCTGCCTCAG CTCATGCGGG ATGTCCAGGT GAACCTCGGC
    TACCTGTGCC AGGCCTGCAC CTCCCTCCTG CACAGCCGCA AGATGCTGCA GCACTACCTG
    CAGAACAAAA ATGGGGAGGG CATCCCCTCA GCTGTCGCCC CCCGAGCTCA GCGGCCACCC
    ACAGCTGCCC CGGCTGCCCC CTCCTGCTCC TCCTCCAAGC AGCCTGCTCC CGACACGGAG
    GCCTCGGAAC AGCGGGCCTT GCGCACCGTC CAGTATGGCC TCCTGAGGAT CCTCAGCAGG
    ACCCTGGCCG CCCTGCGCCG CTTCACCCCC GACGTCTGCC AGGTCCTGCT GGATCAGTCC
    CTGGACCTTG CTGAGTACAG CTTCCTGTTT GCCCTGAGCT TCACCACTCC CACTTTTGAC
    TCTGAAGGGG CCCCCTCCTT CGGGACCCTT CTGGCCACCG TGAATGTGGC CCTGAACATG
    CTTGGAGAGC TGGACAAGAA AAAGGAGCCT CTCACCCAGG CTGTGGGGCT CAGCACACAA
    GCAGAAGGGA CCAGAACTTT GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006065386.1
    CDS96..5162
    Translation

    Target ORF information:

    RefSeq Version XM_006065324.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis nucleoporin 188kDa (NUP188), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006065324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTTGGCCC CGCCCCCGCA GCCACGTGAC GAAAACCCAG TTCACGCCCT GGAAGCGCGC 
    GGTCACGTGG GCGTGGCGTC TGGGGGCGGG GCTGGGGCGC GCGCGCGCGC GAAGATGGCG
    GCTGCCGCCG GCGGTCCGTG TGTGAGGAGC AGTAGAGAAC TGTGGACAAT TCTGCTAGGA
    AGGTCAGCTC TTAGAGAACT GAATCAGATT GAGGCAGAGC TGAATAAACA TTGGCAGCGG
    CTGTTAGAAG GACTTTCTTA CTATAAACCT CCCAGTCCAA GTTCAGCTGA AAAAGTGAAA
    GCTAGTAAAG ATGTAGCTTC ACCACTGAAG GAACTGGGTT TAAGAATCAG CAAGTTTTTG
    GGTCTTGATG AAGAACAGAG TGTGCAGTTA CTCCAGTGTT ACCTTCAAGA GGATTACAGA
    GGTACTCGGG ACTCACTAAA GACGGTGCTG CAAGATGAGA GGCAGAGCCA GGCCTTAATC
    CTGAAGATTG CAGATTATTA CTATGAAGAA AGAACTTGTA TTCTTCGTTG CGTCTTACAC
    CTTCTCACTT ATTTCCAAGA TGAAAGACAC CCCTATAGGG TTGAATATGC CGACTGTGTG
    GATAAATTGG AAAAGGAGCT AGTTACAAAA TATAGACAGC AGTTTGAAGA GCTTTATAAA
    ACTGAAGCAC CAACGTGGGA GACACATGGA AACCTCATGA CTGAGCGCCA GGTGTCTCGC
    TGGTTTGTTC AGTGCCTTCG GGAGCAGTCC ATGCTGCTAG AGATTATTTT CCTGTATTAT
    GCATACTTTG AGATGGCACC TAGTGACCTA TTGGTGTTAA CCAAGATGTT TAAAGAGCAA
    GGATTTGGTA GTAGGCAGAC TAACAGGCAC CTGGTAGATG AGACCATGGA TCCTTTTGTG
    GATCGGATTG GCTACTTCAG TGCCCTTATT CTGGTGGAAG GCATGGACAT TGAATCCTTG
    CATAAGTGTG CTTTGGATGA CAGAAGAGAA CTGCACCAGT TTGCCCAGGA TGGACTTATT
    TGTCAGGATA TGGACCGTTT GATGTTGACC TTTGGGGACA TTCCGCATCA TGCCCCAGTG
    CTTTTGGCAT GGGCCCTCCT CCGTCACACC CTGAATCCAG AAGAGACGAG CAGTGTGGTC
    CGGAAGATAG GGGGGACAGC CATCCAACTG AACGTCTTTC AGTACTTGAC CCGACTGCTC
    CGCTCGCTGG CCAGTGGGGG AAATGACTGC ACCACCAGCA CTGCCTGCAT GTGTGTCTAC
    GGACTCCTTT CTTTTGTCCT GACCTCCCTG GAGCTGCACA CCCTGGGCAA TCAGCAGGAT
    GTAATTGACA CAGCATGTGA AGTCCTGGCA GACCCTTCTC TCCCAGAACT CTTCTGGGGA
    ACAGAACCAA CCTCTGGTCT CGGGATCATT CTGGACAGTG TATGTGGAAT GTTTCCCCAT
    CTCCTCTCTC CACTCCTGCA GTTGCTTCGA GCCCTTGTAT CAGGGAAGTC TACTGCTAAG
    AAGGTGTATA GTTTCTTGGA TAAGATGTCT TTCTACAATG AACTCTATAA GCACAAGCCC
    CATGATGTGA TCTCCCATGA AGATGGAACC CTTTGGCGGA GGCAAACACC CAAACTCCTT
    TACCCTCTAG GGGGTCAGAC CAACCTTCGC ATACCTCAAG GCACCGTGGG CCAAGTGATG
    CTGGACGATA GGGCCTACCT GGTGCGCTGG GAATACTCGT ACAGCAGCTG GACCCTCTTC
    ACCTGCGAGA TTGAGATGCT GCTGCATGTC GTTTCCACTG CAGATGTGAT TCAGCACTGC
    CAGCGGGTCA AACCCATCAT CGACCTGGTC CATAAGGTCA TCAGCACAGA CCTGTCGATA
    GCAGACTGTC TCCTGCCCAT CACGTCCCGC ATCTACATGC TACTGCAGCG GTTAACAACA
    GTCATCTCCC CGCCTGTGGA TGTCATTGCT TCTTGCGTCA ATTGCTTGAC TGTTTTGGCT
    GCCCGGAACC CAGCAAAGGT CTGGACTGAT CTTCTTCACA CAGGCTTCTT GCCTTTCGTG
    GCCCACCAGG TCTCCAGCAT GAGTCAGATG ATCAGTGCTG AGGGGATGAA TGCTGGTGGG
    TATGGAAACC TCTTGATGAA TAGTGAACAG CCCCAGGGCG AGTATGGGGT CACTGTTGCC
    TTTCTGCGCT TGATCACCAC TCTTGTGAAG GGGCAGCTTG GTAGTACCCA GAGCCAAGGG
    CTGGTGCCTT GTGTCATGTT TGTGCTGAAG GAGATGCTGC CAAGTTACCA CAAGTGGCGC
    TACAACTCCC ATGGTGTGAG AGAACAGATT GGTTGCCTGA TCCTGGAGCT GATTCATTCA
    ATACTGAACC TGTGCCATGA GACAGACCTG CATAGCAGTC ACACCCCCAG CCTGCAGTCT
    CTCTGCATCT GCAGCCTGGC CTACACAGAA GCAGGACAGA CAGTAATCAA TATCATGGGC
    ATCGGTGTGG ACACCATCGA CATGGTCATG GCTGCTCAGC CCCGCAGCGA TGGGGCAGAG
    GGCCAGGGCC AGGGCCAGCT GCTGATCAAG ACAGTGAAAT TGGCGTTCTC CGTCACCAAC
    AATGTCATTC GGCTGAAACC GCCTTCTAAT GTGGTGTCCC CTCTGGAACA GGCTCTCACA
    CAGCATGGTG CCCATGGGAA CAACCTTATT GCTGTTCTTG CCAAATACAT CTACCACAAA
    CACGACCCAG CTCTGCCTCG TCTTGCCATC CAGCTGCTGA AACGTCTGGC CACGGTGGCC
    CCCATGTCAG TGTATGCCTG CCTGGGCAGT GATGCGGCTG CCATCCGAGA CGCCTTCCTG
    ACCCGACTGC AGAGCAAGAT TGAGGATATG CGCATCAAAG TCATGATTCT GGAATTCCTC
    ACGGTTGCTG TAGAGACGCA GCCAGGCCTC ATTGAGCTGT TTCTGAATCT GGAGGTGAAG
    GATGGCAGCG ATGGCTCCAA GGAATTCAGC CTGGGGGTGT GGAGCTGTCT CCACGCGGTG
    CTGGAGCTGA TCGGTTCCCA GCAGCAGGAC CGCTACTGGT GCCCCCCACT GCTGCACCGG
    GCAGCCATCG CCTTTTTGCA CGCCCTGTGG CAGGACCGGC GGGACAGTGC CATGCTGGTC
    CTCAGAACCA AACCCAAGTT TTGGGAGAAT CTAACGAGTC CGCTATTTGG TACTCTTTCT
    CCTCCCTCGG AAACATCTGA GCCCAGCATC CTGGAAACCT GTGCCCTAAT CATGAAGATA
    ATTTGCTTGG AGATATACTA TGTAGTTAAG GGCTCATTAG ACCAATCATT AAAAGATACA
    CTCAAGAAAT TTTCCAGCGA GAAGCGCTTT GCCTACTGGT CGGGGTATGT CAAGTCCTTG
    GCGGTTCACA TGGCCGACAC AGAGGGCAGC AGCTGCACCT CCCTGGTAGA GTATCAGATG
    CTGGTCTCTG CCTGGAGGAT GTTTCTCATC ATTGCCACCA GTCACGCGGA GATAACGCAC
    CTGACAGACT CTGCAGTGCG TCGCCAGCTC TTTCTTGACG TGCTCGAGGG GACCAAGGCG
    TTACTCCTCG TCCCTACCTC CGTGAACTGC CTCCGCCTCG GCTCCATGAC GTGCACCCTG
    CTGCTTATTC TCCTCCGGCA GTGGAAAAGA GAGTTAGGGT CTGTGGATGA AATCCTCGGG
    CCCCTGACGG AGATCCTGGA GGGAGTGCTG CAGGCAGACC AGCAGCTTAT GGAGAAGACC
    AAGGCCAAGG TGTTCTCAGC GTTCATCACG GTGCTGCAGA TGAAGGAGAT GAGCGTGAAT
    GACATCCCCC AGTACTCCCA GCTGGTGCTG AACGTCTGTG AGACCCTTCA GGAAGAGGTG
    ATCGCACTTT TCGACCACAC CCGCCACAGC CTGGCGGCGG GCAGTGCCAC CGAGGACAAG
    GACAGCATGG AGACCGACGA CTGCTCCCGG CTCCGGCACA AGGACCAGCG GGACGGGGTC
    TGTGTCCTGG GCCTGCACCT GGCCAAGGAG CTGTGTGAGG TGGATGAGGA CGGGGACTCG
    TGGCTGCAGG TGACCCGCCG GCTCCCCATC CTGCCGACCC TCTTCACCAC CTTGGAGGTG
    AGCCTGCGCG TGAAGCAGAA CCTGCACTTC ACCGAGGCGG CGCTGCACCT GCTCCTCACG
    CTGGCCCGCA CCCAGCAGGG GGCCACAGCC GTGGCCGGGG CCGGCGTCAC CCAGAGCATT
    TGCTTGCCTC TCCTGAGTGT GTACCAGCTC AGCGCCAACG GGACAGTGCA GACACCCGGC
    ACCTCTCGGA AGTCCCTGGA CGCCCCCTCT TGGCCTGGCG TCTACCGTCT CTCCATGTCC
    TTGATGGAGC GGCTCCTCAA AACCCTGCGC TACAACTTCC TGACCGAGGC CCTGGACTTC
    GTGGGGGTTC ACCAGGAGCG GACCTTACAG TGCCTCAATG CCGTGAGGAC GGTGCAGAGT
    CTGGCGTGCC TGGAGGAGGC TGACCACACC GTGGGCTTCC TCCTACAGCT CTCCAGCTTC
    AGGAAGGAGT GGCACTTCCA CCTGCCTCAG CTCATGCGGG ATGTCCAGGT GAACCTCGGC
    TACCTGTGCC AGGCCTGCAC CTCCCTCCTG CACAGCCGCA AGATGCTGCA GCACTACCTG
    CAGAACAAAA ATGGGGAGGG CATCCCCTCA GCTGTCGCCC CCCGAGCTCA GCGGCCACCC
    ACAGCTGCCC CGGCTGCCCC CTCCTGCTCC TCCTCCAAGC AGCCTGCTCC CGACACGGAG
    GCCTCGGAAC AGCGGGCCTT GCGCACCGTC CAGTATGGCC TCCTGAGGAT CCTCAGCAGG
    ACCCTGGCCG CCCTGCGCCG CTTCACCCCC GACGTCTGCC AGGTCCTGCT GGATCAGTCC
    CTGGACCTTG CTGAGTACAG CTTCCTGTTT GCCCTGAGCT TCACCACTCC CACTTTTGAC
    TCTGAAGGGG CCCCCTCCTT CGGGACCCTT CTGGCCACCG TGAATGTGGC CCTGAACATG
    CTTGGAGAGC TGGACAAGAA AAAGGAGCCT CTCACCCAGG CTGTGGGGCT CAGCACACAA
    GCAGAAGGGA CCAGAACTTT GAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.