DGKA gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006075067.1	XP_006075129.1	diacylglycerol kinase alpha isoform X1
XM_006075071.1	XP_006075133.1	diacylglycerol kinase alpha isoform X5
XM_006075067.2	XP_006075129.1	diacylglycerol kinase alpha isoform X1
XM_006075069.2	XP_006075131.1	diacylglycerol kinase alpha isoform X3
XM_006075068.1	XP_006075130.1	diacylglycerol kinase alpha isoform X2
XM_006075068.2	XP_006075130.1	diacylglycerol kinase alpha isoform X2
XM_025283707.1	XP_025139492.1	diacylglycerol kinase alpha isoform X3
XM_006075069.1	XP_006075131.1	diacylglycerol kinase alpha isoform X3
XM_006075070.1	XP_006075132.1	diacylglycerol kinase alpha isoform X4
XM_006075070.2	XP_006075132.1	diacylglycerol kinase alpha isoform X3
XM_025283709.1	XP_025139494.1	diacylglycerol kinase alpha isoform X3
XM_006075072.1	XP_006075134.1	diacylglycerol kinase alpha isoform X4
XM_025283708.1	XP_025139493.1	diacylglycerol kinase alpha isoform X3
XM_006075071.2	XP_006075133.1	diacylglycerol kinase alpha isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu28853	XM_006075067.2 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28854	XM_006075068.2 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_025283707.1 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_006075071.2 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_006075069.2 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_025283709.1 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_025283708.1 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_006075070.2 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28856	XM_006075072.1 Latest version!	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBu28853	XM_006075067.1	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28854	XM_006075068.1	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_006075069.1	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_006075070.1	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu28855	XM_006075071.1	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

DGKA ( XM_006075067.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075129.1 Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu28853
Clone ID Related Accession (Same CDS sequence)	XM_006075067.1 , XM_006075067.2
Accession Version	XM_006075067.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAGA TGGAGTCAAG GATGAGTCAG AGAAACAGAC ACAGAGAACA GACACTGTCA 
GGGAGAGATG GAGACACAAA GAACTGCCCT TGGAAGCAAA GTTCCAAGGC CCAGAAGTAC 
TACAAACAAG CTGCCTTTGT GTTCATCCTT AAGAAAAAAA GAAAGATGGC CAAGGAGAGG 
GGCCTAATAA GCCCCAGTGA TTTTGCCCAG CTGCAAAAAT ACATGGAATA CTCCACCAAA 
AAGGTCAGTG ATGTTCTGAA GCTCTTTGAG GATGGTGAAA TGGCTGAATA TCTCCAAGGA 
GATGCCATTG GATACGAGGG GTTCCAGCAA TTCCTGAAAA TCTACCTGGA AGTGGATAAT 
GTTCCAGATC ACCTAAGCCA GGCACTGTTT CAGTCCTTCC AGACTGGCTA CTACGTAGAA 
GATACTGTAA GAGAAGATGT GGTGTGTCTC AGTGATGTCT CCTGCTACTT TTCTCTTCTG 
GAGGGTGGCC GGCCGGAAGA CAAGCTAGAA TTCACCTTCA AGCTGTACGA CACGGACAGA 
AATGGGATCC TGGACAGCTC AGAAGTGGAC AGGATCATCA TACAGATGAT GCGAATGGCT 
GAATACCTGG ATTGGGATGT GTCTGAGCTG AGGCCGATTC TTCAAGAGAT GATGAAGGAG 
ATTGACTATG ATGGCAGTGG CTCTGTGTCC CTGGCTGAGT GGCTCCGGGC TGGGGCCACC 
ACCGTGCCAC TGCTCGTGCT GCTGGGCCTG GAGATGACCC TGAAGGACAA TGGGCAGCAC 
ATGTGGAGAC CCAAGAGATT CCCCCGACCA GTCTACTGCA ACCTGTGCGA GTCGAGCATT 
GGTCTTGGCA AACAGGGGCT GAGCTGTAAC CTGTGTAAAT ACATCGTTCA TGACCAATGT 
GCCATGAAGG CCCTGCCCTG TGAAGTCAGC ACCTATGCCA AGTCTCGGAA GGACATTGGT 
GTCCAATCAC ATGTGTGGGT GCGAGGAGGC TGTGAATCTG GACGGTGTGA CCGCTGTCAG 
AAAAAGATCC GGATCTACCA CAGTCTGGTT GGGCTGCATT GTGTGTGGTG CCACCTAGAG 
ATCCATGATG ACTGCCTTCC AGCCATGGGC CATGAGTGTG ACTGTGGGCT GCTCCGAGAT 
CACATCCTGC CTCCGTCTTC CATCTATCCC AGCGTCCTGG CCTCTGGACA GGAGCGTAAA 
ACTAGCAAAA TAAGCCAGAA GACCATGGAT GATTTAAGTT TGAGCACGTC TGAGGCTCTG 
CGGATTGATC CCGTTTCTAA CACCCACCCA CTTCTGGTCT TTGTGAACCC CAAGAGTGGC 
GGGAAGCAAG GCGAGAGGGT GCTTTGGAAA TTCCAGTATC TGCTGAACCC TCGACAGGTG 
TTCAACCTCC TAAAGGATGG TCCTGAGCCA GGACTCAGAT TCTTCAGAGA TGTTCCTGAT 
TACAGGATTT TGGTGTGCGG CGGAGATGGC ACAGTAGGCT GGATTCTAGA GTCCATTGAC 
AAAGCCAACT TGCCTTTTGT GCCTCCTGTC GCTGTGTTGC CCCTGGGCAC TGGAAATGAT 
CTGGCTCGTT GCCTAAGATG GGGAGGAGGG TATGAGGGAC AGAATCTGGG GAAGATCCTC 
AAGGATTTAG AGACAAGTAA GGTGGTACAT ATGGATCGAT GGTCTGTGGA GGTGATACCC 
CAACAAACTG AAGAAAAGAG TGACCCAGTC CCCTTTCAAA TCATCAATAA CTACTTCTCC 
ATCGGTGTGG ATGCCTCTAT TGCTCACCGA TTCCACATTA TGCGAGAGAA ATATCCTGAG 
AAGTTCAACA GCAGAATGAA GAACAAGCTA TGGTACTTCG AATTTGCCAC TTCTGAATCC 
ATCTTCTCAA CATGCAAAAA GCTGGAGGAA TCTTTGACAG TTGAGATCTG TGGGAAACCA 
CTGGATCTGA GCAACCTGTC CCTAGAAGGC ATTGCAGTGC TGAACATCCC AAGCACACAT 
GGTGGCTCCA ATCTCTGGGG TGACACCAAG AGACCACATG GCGATATCCA TGGGATCAAC 
CAGGCCTTAG GTGCTACTGC CAAAGTCATC ACCGACCCTG ATATCCTAAA GACCTGTGTA 
CCAGACCTAA GTGACAAGCG GCTGGAAGTA GTGGGGCTGG AGGGTGCAAT TGAGATAGGC 
CAGATCTATA CCAAGCTCAA GAATGCTGGA CATCGGCTGG CCAAGTGCTC TGAGATCACC 
TTCCACACCA CAAAAACCCT CCCCATGCAA ATTGACGGAG AACCCTGGAT GCAGACACCT 
TGTACAATCA AGATCACCCA CAGGAACCAG ATGCCCATGC TCGTGGGCCC ACCCCCTCGC 
TCCTCCAATT TCTTTGGCTT CTTGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075129.1
CDS	210..2579
Translation	MLEMESRMSQRNRHREQTLSGRDGDTKNCPWKQSSKAQKYYKQAAFVFILKKKRKMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075067.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075067.1

ORF Insert Sequence:

ATGTTGGAGA TGGAGTCAAG GATGAGTCAG AGAAACAGAC ACAGAGAACA GACACTGTCA 
GGGAGAGATG GAGACACAAA GAACTGCCCT TGGAAGCAAA GTTCCAAGGC CCAGAAGTAC 
TACAAACAAG CTGCCTTTGT GTTCATCCTT AAGAAAAAAA GAAAGATGGC CAAGGAGAGG 
GGCCTAATAA GCCCCAGTGA TTTTGCCCAG CTGCAAAAAT ACATGGAATA CTCCACCAAA 
AAGGTCAGTG ATGTTCTGAA GCTCTTTGAG GATGGTGAAA TGGCTGAATA TCTCCAAGGA 
GATGCCATTG GATACGAGGG GTTCCAGCAA TTCCTGAAAA TCTACCTGGA AGTGGATAAT 
GTTCCAGATC ACCTAAGCCA GGCACTGTTT CAGTCCTTCC AGACTGGCTA CTACGTAGAA 
GATACTGTAA GAGAAGATGT GGTGTGTCTC AGTGATGTCT CCTGCTACTT TTCTCTTCTG 
GAGGGTGGCC GGCCGGAAGA CAAGCTAGAA TTCACCTTCA AGCTGTACGA CACGGACAGA 
AATGGGATCC TGGACAGCTC AGAAGTGGAC AGGATCATCA TACAGATGAT GCGAATGGCT 
GAATACCTGG ATTGGGATGT GTCTGAGCTG AGGCCGATTC TTCAAGAGAT GATGAAGGAG 
ATTGACTATG ATGGCAGTGG CTCTGTGTCC CTGGCTGAGT GGCTCCGGGC TGGGGCCACC 
ACCGTGCCAC TGCTCGTGCT GCTGGGCCTG GAGATGACCC TGAAGGACAA TGGGCAGCAC 
ATGTGGAGAC CCAAGAGATT CCCCCGACCA GTCTACTGCA ACCTGTGCGA GTCGAGCATT 
GGTCTTGGCA AACAGGGGCT GAGCTGTAAC CTGTGTAAAT ACATCGTTCA TGACCAATGT 
GCCATGAAGG CCCTGCCCTG TGAAGTCAGC ACCTATGCCA AGTCTCGGAA GGACATTGGT 
GTCCAATCAC ATGTGTGGGT GCGAGGAGGC TGTGAATCTG GACGGTGTGA CCGCTGTCAG 
AAAAAGATCC GGATCTACCA CAGTCTGGTT GGGCTGCATT GTGTGTGGTG CCACCTAGAG 
ATCCATGATG ACTGCCTTCC AGCCATGGGC CATGAGTGTG ACTGTGGGCT GCTCCGAGAT 
CACATCCTGC CTCCGTCTTC CATCTATCCC AGCGTCCTGG CCTCTGGACA GGAGCGTAAA 
ACTAGCAAAA TAAGCCAGAA GACCATGGAT GATTTAAGTT TGAGCACGTC TGAGGCTCTG 
CGGATTGATC CCGTTTCTAA CACCCACCCA CTTCTGGTCT TTGTGAACCC CAAGAGTGGC 
GGGAAGCAAG GCGAGAGGGT GCTTTGGAAA TTCCAGTATC TGCTGAACCC TCGACAGGTG 
TTCAACCTCC TAAAGGATGG TCCTGAGCCA GGACTCAGAT TCTTCAGAGA TGTTCCTGAT 
TACAGGATTT TGGTGTGCGG CGGAGATGGC ACAGTAGGCT GGATTCTAGA GTCCATTGAC 
AAAGCCAACT TGCCTTTTGT GCCTCCTGTC GCTGTGTTGC CCCTGGGCAC TGGAAATGAT 
CTGGCTCGTT GCCTAAGATG GGGAGGAGGG TATGAGGGAC AGAATCTGGG GAAGATCCTC 
AAGGATTTAG AGACAAGTAA GGTGGTACAT ATGGATCGAT GGTCTGTGGA GGTGATACCC 
CAACAAACTG AAGAAAAGAG TGACCCAGTC CCCTTTCAAA TCATCAATAA CTACTTCTCC 
ATCGGTGTGG ATGCCTCTAT TGCTCACCGA TTCCACATTA TGCGAGAGAA ATATCCTGAG 
AAGTTCAACA GCAGAATGAA GAACAAGCTA TGGTACTTCG AATTTGCCAC TTCTGAATCC 
ATCTTCTCAA CATGCAAAAA GCTGGAGGAA TCTTTGACAG TTGAGATCTG TGGGAAACCA 
CTGGATCTGA GCAACCTGTC CCTAGAAGGC ATTGCAGTGC TGAACATCCC AAGCACACAT 
GGTGGCTCCA ATCTCTGGGG TGACACCAAG AGACCACATG GCGATATCCA TGGGATCAAC 
CAGGCCTTAG GTGCTACTGC CAAAGTCATC ACCGACCCTG ATATCCTAAA GACCTGTGTA 
CCAGACCTAA GTGACAAGCG GCTGGAAGTA GTGGGGCTGG AGGGTGCAAT TGAGATAGGC 
CAGATCTATA CCAAGCTCAA GAATGCTGGA CATCGGCTGG CCAAGTGCTC TGAGATCACC 
TTCCACACCA CAAAAACCCT CCCCATGCAA ATTGACGGAG AACCCTGGAT GCAGACACCT 
TGTACAATCA AGATCACCCA CAGGAACCAG ATGCCCATGC TCGTGGGCCC ACCCCCTCGC 
TCCTCCAATT TCTTTGGCTT CTTGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075067.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075129.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu28853
Clone ID Related Accession (Same CDS sequence)	XM_006075067.1 , XM_006075067.2
Accession Version	XM_006075067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006075067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAGA TGGAGTCAAG GATGAGTCAG AGAAACAGAC ACAGAGAACA GACACTGTCA 
GGGAGAGATG GAGACACAAA GAACTGCCCT TGGAAGCAAA GTTCCAAGGC CCAGAAGTAC 
TACAAACAAG CTGCCTTTGT GTTCATCCTT AAGAAAAAAA GAAAGATGGC CAAGGAGAGG 
GGCCTAATAA GCCCCAGTGA TTTTGCCCAG CTGCAAAAAT ACATGGAATA CTCCACCAAA 
AAGGTCAGTG ATGTTCTGAA GCTCTTTGAG GATGGTGAAA TGGCTGAATA TCTCCAAGGA 
GATGCCATTG GATACGAGGG GTTCCAGCAA TTCCTGAAAA TCTACCTGGA AGTGGATAAT 
GTTCCAGATC ACCTAAGCCA GGCACTGTTT CAGTCCTTCC AGACTGGCTA CTACGTAGAA 
GATACTGTAA GAGAAGATGT GGTGTGTCTC AGTGATGTCT CCTGCTACTT TTCTCTTCTG 
GAGGGTGGCC GGCCGGAAGA CAAGCTAGAA TTCACCTTCA AGCTGTACGA CACGGACAGA 
AATGGGATCC TGGACAGCTC AGAAGTGGAC AGGATCATCA TACAGATGAT GCGAATGGCT 
GAATACCTGG ATTGGGATGT GTCTGAGCTG AGGCCGATTC TTCAAGAGAT GATGAAGGAG 
ATTGACTATG ATGGCAGTGG CTCTGTGTCC CTGGCTGAGT GGCTCCGGGC TGGGGCCACC 
ACCGTGCCAC TGCTCGTGCT GCTGGGCCTG GAGATGACCC TGAAGGACAA TGGGCAGCAC 
ATGTGGAGAC CCAAGAGATT CCCCCGACCA GTCTACTGCA ACCTGTGCGA GTCGAGCATT 
GGTCTTGGCA AACAGGGGCT GAGCTGTAAC CTGTGTAAAT ACATCGTTCA TGACCAATGT 
GCCATGAAGG CCCTGCCCTG TGAAGTCAGC ACCTATGCCA AGTCTCGGAA GGACATTGGT 
GTCCAATCAC ATGTGTGGGT GCGAGGAGGC TGTGAATCTG GACGGTGTGA CCGCTGTCAG 
AAAAAGATCC GGATCTACCA CAGTCTGGTT GGGCTGCATT GTGTGTGGTG CCACCTAGAG 
ATCCATGATG ACTGCCTTCC AGCCATGGGC CATGAGTGTG ACTGTGGGCT GCTCCGAGAT 
CACATCCTGC CTCCGTCTTC CATCTATCCC AGCGTCCTGG CCTCTGGACA GGAGCGTAAA 
ACTAGCAAAA TAAGCCAGAA GACCATGGAT GATTTAAGTT TGAGCACGTC TGAGGCTCTG 
CGGATTGATC CCGTTTCTAA CACCCACCCA CTTCTGGTCT TTGTGAACCC CAAGAGTGGC 
GGGAAGCAAG GCGAGAGGGT GCTTTGGAAA TTCCAGTATC TGCTGAACCC TCGACAGGTG 
TTCAACCTCC TAAAGGATGG TCCTGAGCCA GGACTCAGAT TCTTCAGAGA TGTTCCTGAT 
TACAGGATTT TGGTGTGCGG CGGAGATGGC ACAGTAGGCT GGATTCTAGA GTCCATTGAC 
AAAGCCAACT TGCCTTTTGT GCCTCCTGTC GCTGTGTTGC CCCTGGGCAC TGGAAATGAT 
CTGGCTCGTT GCCTAAGATG GGGAGGAGGG TATGAGGGAC AGAATCTGGG GAAGATCCTC 
AAGGATTTAG AGACAAGTAA GGTGGTACAT ATGGATCGAT GGTCTGTGGA GGTGATACCC 
CAACAAACTG AAGAAAAGAG TGACCCAGTC CCCTTTCAAA TCATCAATAA CTACTTCTCC 
ATCGGTGTGG ATGCCTCTAT TGCTCACCGA TTCCACATTA TGCGAGAGAA ATATCCTGAG 
AAGTTCAACA GCAGAATGAA GAACAAGCTA TGGTACTTCG AATTTGCCAC TTCTGAATCC 
ATCTTCTCAA CATGCAAAAA GCTGGAGGAA TCTTTGACAG TTGAGATCTG TGGGAAACCA 
CTGGATCTGA GCAACCTGTC CCTAGAAGGC ATTGCAGTGC TGAACATCCC AAGCACACAT 
GGTGGCTCCA ATCTCTGGGG TGACACCAAG AGACCACATG GCGATATCCA TGGGATCAAC 
CAGGCCTTAG GTGCTACTGC CAAAGTCATC ACCGACCCTG ATATCCTAAA GACCTGTGTA 
CCAGACCTAA GTGACAAGCG GCTGGAAGTA GTGGGGCTGG AGGGTGCAAT TGAGATAGGC 
CAGATCTATA CCAAGCTCAA GAATGCTGGA CATCGGCTGG CCAAGTGCTC TGAGATCACC 
TTCCACACCA CAAAAACCCT CCCCATGCAA ATTGACGGAG AACCCTGGAT GCAGACACCT 
TGTACAATCA AGATCACCCA CAGGAACCAG ATGCCCATGC TCGTGGGCCC ACCCCCTCGC 
TCCTCCAATT TCTTTGGCTT CTTGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075129.1
CDS	70..2439
Translation	MLEMESRMSQRNRHREQTLSGRDGDTKNCPWKQSSKAQKYYKQAAFVFILKKKRKMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075067.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075067.2

ORF Insert Sequence:

ATGTTGGAGA TGGAGTCAAG GATGAGTCAG AGAAACAGAC ACAGAGAACA GACACTGTCA 
GGGAGAGATG GAGACACAAA GAACTGCCCT TGGAAGCAAA GTTCCAAGGC CCAGAAGTAC 
TACAAACAAG CTGCCTTTGT GTTCATCCTT AAGAAAAAAA GAAAGATGGC CAAGGAGAGG 
GGCCTAATAA GCCCCAGTGA TTTTGCCCAG CTGCAAAAAT ACATGGAATA CTCCACCAAA 
AAGGTCAGTG ATGTTCTGAA GCTCTTTGAG GATGGTGAAA TGGCTGAATA TCTCCAAGGA 
GATGCCATTG GATACGAGGG GTTCCAGCAA TTCCTGAAAA TCTACCTGGA AGTGGATAAT 
GTTCCAGATC ACCTAAGCCA GGCACTGTTT CAGTCCTTCC AGACTGGCTA CTACGTAGAA 
GATACTGTAA GAGAAGATGT GGTGTGTCTC AGTGATGTCT CCTGCTACTT TTCTCTTCTG 
GAGGGTGGCC GGCCGGAAGA CAAGCTAGAA TTCACCTTCA AGCTGTACGA CACGGACAGA 
AATGGGATCC TGGACAGCTC AGAAGTGGAC AGGATCATCA TACAGATGAT GCGAATGGCT 
GAATACCTGG ATTGGGATGT GTCTGAGCTG AGGCCGATTC TTCAAGAGAT GATGAAGGAG 
ATTGACTATG ATGGCAGTGG CTCTGTGTCC CTGGCTGAGT GGCTCCGGGC TGGGGCCACC 
ACCGTGCCAC TGCTCGTGCT GCTGGGCCTG GAGATGACCC TGAAGGACAA TGGGCAGCAC 
ATGTGGAGAC CCAAGAGATT CCCCCGACCA GTCTACTGCA ACCTGTGCGA GTCGAGCATT 
GGTCTTGGCA AACAGGGGCT GAGCTGTAAC CTGTGTAAAT ACATCGTTCA TGACCAATGT 
GCCATGAAGG CCCTGCCCTG TGAAGTCAGC ACCTATGCCA AGTCTCGGAA GGACATTGGT 
GTCCAATCAC ATGTGTGGGT GCGAGGAGGC TGTGAATCTG GACGGTGTGA CCGCTGTCAG 
AAAAAGATCC GGATCTACCA CAGTCTGGTT GGGCTGCATT GTGTGTGGTG CCACCTAGAG 
ATCCATGATG ACTGCCTTCC AGCCATGGGC CATGAGTGTG ACTGTGGGCT GCTCCGAGAT 
CACATCCTGC CTCCGTCTTC CATCTATCCC AGCGTCCTGG CCTCTGGACA GGAGCGTAAA 
ACTAGCAAAA TAAGCCAGAA GACCATGGAT GATTTAAGTT TGAGCACGTC TGAGGCTCTG 
CGGATTGATC CCGTTTCTAA CACCCACCCA CTTCTGGTCT TTGTGAACCC CAAGAGTGGC 
GGGAAGCAAG GCGAGAGGGT GCTTTGGAAA TTCCAGTATC TGCTGAACCC TCGACAGGTG 
TTCAACCTCC TAAAGGATGG TCCTGAGCCA GGACTCAGAT TCTTCAGAGA TGTTCCTGAT 
TACAGGATTT TGGTGTGCGG CGGAGATGGC ACAGTAGGCT GGATTCTAGA GTCCATTGAC 
AAAGCCAACT TGCCTTTTGT GCCTCCTGTC GCTGTGTTGC CCCTGGGCAC TGGAAATGAT 
CTGGCTCGTT GCCTAAGATG GGGAGGAGGG TATGAGGGAC AGAATCTGGG GAAGATCCTC 
AAGGATTTAG AGACAAGTAA GGTGGTACAT ATGGATCGAT GGTCTGTGGA GGTGATACCC 
CAACAAACTG AAGAAAAGAG TGACCCAGTC CCCTTTCAAA TCATCAATAA CTACTTCTCC 
ATCGGTGTGG ATGCCTCTAT TGCTCACCGA TTCCACATTA TGCGAGAGAA ATATCCTGAG 
AAGTTCAACA GCAGAATGAA GAACAAGCTA TGGTACTTCG AATTTGCCAC TTCTGAATCC 
ATCTTCTCAA CATGCAAAAA GCTGGAGGAA TCTTTGACAG TTGAGATCTG TGGGAAACCA 
CTGGATCTGA GCAACCTGTC CCTAGAAGGC ATTGCAGTGC TGAACATCCC AAGCACACAT 
GGTGGCTCCA ATCTCTGGGG TGACACCAAG AGACCACATG GCGATATCCA TGGGATCAAC 
CAGGCCTTAG GTGCTACTGC CAAAGTCATC ACCGACCCTG ATATCCTAAA GACCTGTGTA 
CCAGACCTAA GTGACAAGCG GCTGGAAGTA GTGGGGCTGG AGGGTGCAAT TGAGATAGGC 
CAGATCTATA CCAAGCTCAA GAATGCTGGA CATCGGCTGG CCAAGTGCTC TGAGATCACC 
TTCCACACCA CAAAAACCCT CCCCATGCAA ATTGACGGAG AACCCTGGAT GCAGACACCT 
TGTACAATCA AGATCACCCA CAGGAACCAG ATGCCCATGC TCGTGGGCCC ACCCCCTCGC 
TCCTCCAATT TCTTTGGCTT CTTGTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075068.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075130.1 Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu28854
Clone ID Related Accession (Same CDS sequence)	XM_006075068.1 , XM_006075068.2
Accession Version	XM_006075068.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075130.1
CDS	112..2436
Translation	MGSWPGRRAAISNCPWKQSSKAQKYYKQAAFVFILKKKRKMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075068.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075068.1

ORF Insert Sequence:

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075068.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075130.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu28854
Clone ID Related Accession (Same CDS sequence)	XM_006075068.1 , XM_006075068.2
Accession Version	XM_006075068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006075068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075130.1
CDS	105..2429
Translation	MGSWPGRRAAISNCPWKQSSKAQKYYKQAAFVFILKKKRKMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075068.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075068.2

ORF Insert Sequence:

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075071.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075133.1 Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_006075071.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075133.1
CDS	179..2383
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075071.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075071.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075069.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075131.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_006075069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006075069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075131.1
CDS	411..2615
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075069.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075069.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_025283707.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139492.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_025283707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139492.1
CDS	280..2484
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_025283707.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283707.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075069.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075131.1 Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_006075069.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075131.1
CDS	174..2378
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075069.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075069.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075070.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075132.1 Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_006075070.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785618.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075132.1
CDS	301..2505
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075070.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase, alpha 80kDa (DGKA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075070.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075070.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075132.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_006075070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006075070.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075132.1
CDS	309..2513
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075070.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075070.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_025283709.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139494.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_025283709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139494.1
CDS	267..2471
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_025283709.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283709.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_025283708.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025139493.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_025283708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025139493.1
CDS	349..2553
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_025283708.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025283708.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075071.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075133.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu28855
Clone ID Related Accession (Same CDS sequence)	XM_006075071.1 , XM_006075069.2 , XM_025283707.1 , XM_006075069.1 , XM_006075070.1 , XM_006075070.2 , XM_025283709.1 , XM_025283708.1 , XM_006075071.2
Accession Version	XM_006075071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006075071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075133.1
CDS	206..2410
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYVEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075071.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075071.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAAGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACG TAGAAGATAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCG GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_006075072.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006075134.1 Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu28856
Clone ID Related Accession (Same CDS sequence)	XM_006075072.1
Accession Version	XM_006075072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	diacylglycerol kinase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006075134.1
CDS	100..1884
Translation	MMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_006075072.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis diacylglycerol kinase alpha (DGKA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006075072.1

ORF Insert Sequence:

ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AGGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTGTG TAAATACATC 
GTTCATGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACATGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACGTCTGAGG CTCTGCGGAT TGATCCCGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACATGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTAAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC CATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTCTTT GGCTTCTTGT GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DGKA
Entrez Gene ID	102399428
Full Name	diacylglycerol kinase alpha
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DGKA cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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