PIWIL4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIWIL4
Entrez Gene ID	102398752
Full Name	piwi like RNA-mediated gene silencing 4
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025266963.1	XP_025122748.1	piwi-like protein 4
XM_006048173.1	XP_006048235.1	piwi-like protein 4

Pathways from BioSystems
Gene Ontology

The following PIWIL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIWIL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu50911	XM_025266963.1 Latest version!	Bubalus bubalis piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBu06655	XM_006048173.1 Latest version!	Bubalus bubalis piwi-like RNA-mediated gene silencing 4 (PIWIL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIWIL4 ( XM_025266963.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025122748.1 Bubalus bubalis piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Price & Availability↑

CloneID	OBu50911
Clone ID Related Accession (Same CDS sequence)	XM_025266963.1
Accession Version	XM_025266963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	piwi-like protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037560.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCGA AGAAGCGCCC TCACGGAGAC TTGGCTGCCT CTGGGCTCCG CGGGGACATG 
AGCGGCCGCG CCCGCGTGAG GGCGCGGGGC ATGGCCCGCA GCCCCGGCGC CGCGGAGCCT 
CCATCTGTTG CGCTTATTGA TAATGAAGCA TCCTCTAGCA GTGACTGTGA GAGAACAAGC 
AGGATTTCAC AGAAATGTGG TATATCTCCA AGTGATTCTG GATGTACCTT CCAGGAAAGA 
GGTGGGAAAG TCAGAAGGGA CATCATGGAT TTGGCTGTCT GTACAAGAGA GAAACTGGCC 
CATGTGAGAG ACTGCAAAAC AGGTTCCAGT GGAATACCTG TGAAACTGGT CACAAATCTC 
TTTAATTTAG ACGTGCCCCA GGACTGGCAG CTATACCAGT ATCATGTGAC ATATAGTCCG 
GATATAGAGT CTAGAAGGCT GAGAATTGCT CTGCTTTATA GTCACAGGGA ACTCTCCAAC 
AAAGCAAAAG CATTCGATGG TGTCATCCTT TTTCTCTCAC AAAAGCTAGA AGAAAAGGTC 
ACGGAGTTGT CAAGTGAAAC CCAAAGAGGC GAGACTGTAA AGATGACTAT CACTCTGACG 
AGAGAGCTGC CGGCAAGTTC TCCTGTGTGC ATCCAGGTCT TCAATATCAT CTTCAAAAAG 
ATCTTAAAAA AGTTATCCAT GTACCAAATT GGACGGAACT TCTATAAGCC TTCAGAGCCA 
GTGGAAATTC CCCAGCACAA ATTGTCTCTT TGGCCTGGGT TCGCCATTTC TGTATCACAT 
TTTGAAAGCA AACTCCTGTT GAGTGCTAAT GTGAGTTACA AAATACTGCG GAACGAGACA 
GTTCTGGAGT TCATGACGGA TCTCTGCCGC AAAACTAGCG AGTCCTGCTT TACCCAGACG 
TGTGAGAAAC AGCTGATTGG GCTCATTGTC CTCACAAGAT ACAATAATAA AACCTATCGC 
ATTGATGACA TCGACTGGTC GGTGAAGCCC ACACACACCT TCCGGAAGCG GGACGGCGCT 
GAGATCACCT ACGTGGATTA CTACAAGCAG CAATACGATA TTACCTTATC TGACCTGAAC 
CAGCCAGTTC TTGTTAGTCT GTTGAAAAAC AAGAAAAATG ACAACTCTGA GGCTCAGATG 
GCCCACCTGA TTCCCGAGCT CTGCTTTCTA ACGGGGATGC CTGGCCAGGC GACCTCTGAT 
TTCCAGCTGA TGAAGGCTGT GGCTAAAGAA ACTCGTCTCG ATCCTTTGGT AAGGCAGCAG 
CACCTGGCCA GGCTTGCAGA TGACTTCCAG AGAAACAAGG AGGCTCGTTT TGAGCTGGAG 
ACCTGGGGGC TGCATTTTGG ATGCCAGATG TCTCTGACTG GCCGTATTGC ACCTGCAGAA 
AAAATAATAA TGCAAGACCA CATATGTCAA CCTGCATCTG CAGCTGACTG GTCCAAGGAT 
ATACGAATTT GCAAGGTTTT AAGTGCACAG TCTTTGAATA AATGGCTGGT TATATGTAGT 
AACAGAGCTG AAAGTGTGAC AGAGAACTTT CTGAACTGCT TGAGAAGAGT TGGAGGTCCC 
ATGGGATTTA ATGTGGATTG CCCCAAAATC ATAAAAGTAC AAGAAAATCC AGCTGCATTT 
ATTCACGCTA TACAGAAGCA TGTTAAACAT GAGGTTCAGT TGGTGATGTG TATTCTGCCT 
TCTATTCAGA CCAGCTATTA TGATTCCATT AAAAAATATT TGAGCTCCGA CTGCCCAGTG 
CCCAGCCAGT GTGTGCTTGC TCAGACGTTG AATAAGCAGG GGATGATGCT GAGTGTCGCC 
ACCAAGATTG CCATGCAAAT GATCTGTAAA CTCGGGGGTG AGCTCTGGGC GGTGGAGATC 
CCGTTAAAGT CCCTGATGGT GGTCGGTATC AATGTCTGTA AAGATGCATT CAGCCAGGGA 
ACGATGGTGG TTGGATTTGT GGCCAGTATT AACCCCAGAA TCACCAGGTG GTTTTCCCGC 
TGTATCCTTC AGAGAACAAC AGCTCATGTT GCAGACTGCT TAAAAGTGTT CATGATCGGG 
GCACTGAACA GGTGGTATAC GCATAACCAG GGTTTGCCAG CACGGATAAT TGTGTACCGT 
GAAGGTGTCG GTGACGGTCA GCTCCAGACA CTCGTTGACT ATGAAGTCCC ACAACTGCTG 
AGCAGTGTGA TCGAAGCAAG TTCAAATACC AGCCCCAAAC TGTCGGTGAT TGTGGTCAGG 
AAGAAGGGCA TGCCACGATT CTTGGTGGAA ACTGGCCGAA ATCTGCAGAA CCCACCACTT 
GGCACTGTCG TGGATTCGGA AGCAACACGG CCAGAATGGT ATGACTTTTA CTTGATCAGC 
CAGATGGCCT GTCGGGGAAC CGTTAATCCC ACCTACTATA ATGTCATCTA TGATGACAAT 
GGCCTGAAGC CCGACCACAT GCAGAGACTC ACATTCAAAC TGTGTCACCT GTACTACAAC 
TGGATGGGCC TAATCAGTAT TCCAGCTCCC TGTCAGTATG CACGCAAGCT GACCTTCCTG 
GTGGCACAAA GCCTTCATAA AGAACCAAGT TTGGAATTAG CCAATTCTCT CTTCTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025122748.1
CDS	52..2634
Translation	MPAKKRPHGDLAASGLRGDMSGRARVRARGMARSPGAAEPPSVALIDNEASSSSDCERTSRISQKCGISPSDSGCTFQERGGKVRRDIMDLAVCTREKLAHVRDCKTGSSGIPVKLVTNLFNLDVPQDWQLYQYHVTYSPDIESRRLRIALLYSHRELSNKAKAFDGVILFLSQKLEEKVTELSSETQRGETVKMTITLTRELPASSPVCIQVFNIIFKKILKKLSMYQIGRNFYKPSEPVEIPQHKLSLWPGFAISVSHFESKLLLSANVSYKILRNETVLEFMTDLCRKTSESCFTQTCEKQLIGLIVLTRYNNKTYRIDDIDWSVKPTHTFRKRDGAEITYVDYYKQQYDITLSDLNQPVLVSLLKNKKNDNSEAQMAHLIPELCFLTGMPGQATSDFQLMKAVAKETRLDPLVRQQHLARLADDFQRNKEARFELETWGLHFGCQMSLTGRIAPAEKIIMQDHICQPASAADWSKDIRICKVLSAQSLNKWLVICSNRAESVTENFLNCLRRVGGPMGFNVDCPKIIKVQENPAAFIHAIQKHVKHEVQLVMCILPSIQTSYYDSIKKYLSSDCPVPSQCVLAQTLNKQGMMLSVATKIAMQMICKLGGELWAVEIPLKSLMVVGINVCKDAFSQGTMVVGFVASINPRITRWFSRCILQRTTAHVADCLKVFMIGALNRWYTHNQGLPARIIVYREGVGDGQLQTLVDYEVPQLLSSVIEASSNTSPKLSVIVVRKKGMPRFLVETGRNLQNPPLGTVVDSEATRPEWYDFYLISQMACRGTVNPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWMGLISIPAPCQYARKLTFLVAQSLHKEPSLELANSLFYL

Target ORF information:

RefSeq Version	XM_025266963.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis piwi like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025266963.1

ORF Insert Sequence:

ATGCCGGCGA AGAAGCGCCC TCACGGAGAC TTGGCTGCCT CTGGGCTCCG CGGGGACATG 
AGCGGCCGCG CCCGCGTGAG GGCGCGGGGC ATGGCCCGCA GCCCCGGCGC CGCGGAGCCT 
CCATCTGTTG CGCTTATTGA TAATGAAGCA TCCTCTAGCA GTGACTGTGA GAGAACAAGC 
AGGATTTCAC AGAAATGTGG TATATCTCCA AGTGATTCTG GATGTACCTT CCAGGAAAGA 
GGTGGGAAAG TCAGAAGGGA CATCATGGAT TTGGCTGTCT GTACAAGAGA GAAACTGGCC 
CATGTGAGAG ACTGCAAAAC AGGTTCCAGT GGAATACCTG TGAAACTGGT CACAAATCTC 
TTTAATTTAG ACGTGCCCCA GGACTGGCAG CTATACCAGT ATCATGTGAC ATATAGTCCG 
GATATAGAGT CTAGAAGGCT GAGAATTGCT CTGCTTTATA GTCACAGGGA ACTCTCCAAC 
AAAGCAAAAG CATTCGATGG TGTCATCCTT TTTCTCTCAC AAAAGCTAGA AGAAAAGGTC 
ACGGAGTTGT CAAGTGAAAC CCAAAGAGGC GAGACTGTAA AGATGACTAT CACTCTGACG 
AGAGAGCTGC CGGCAAGTTC TCCTGTGTGC ATCCAGGTCT TCAATATCAT CTTCAAAAAG 
ATCTTAAAAA AGTTATCCAT GTACCAAATT GGACGGAACT TCTATAAGCC TTCAGAGCCA 
GTGGAAATTC CCCAGCACAA ATTGTCTCTT TGGCCTGGGT TCGCCATTTC TGTATCACAT 
TTTGAAAGCA AACTCCTGTT GAGTGCTAAT GTGAGTTACA AAATACTGCG GAACGAGACA 
GTTCTGGAGT TCATGACGGA TCTCTGCCGC AAAACTAGCG AGTCCTGCTT TACCCAGACG 
TGTGAGAAAC AGCTGATTGG GCTCATTGTC CTCACAAGAT ACAATAATAA AACCTATCGC 
ATTGATGACA TCGACTGGTC GGTGAAGCCC ACACACACCT TCCGGAAGCG GGACGGCGCT 
GAGATCACCT ACGTGGATTA CTACAAGCAG CAATACGATA TTACCTTATC TGACCTGAAC 
CAGCCAGTTC TTGTTAGTCT GTTGAAAAAC AAGAAAAATG ACAACTCTGA GGCTCAGATG 
GCCCACCTGA TTCCCGAGCT CTGCTTTCTA ACGGGGATGC CTGGCCAGGC GACCTCTGAT 
TTCCAGCTGA TGAAGGCTGT GGCTAAAGAA ACTCGTCTCG ATCCTTTGGT AAGGCAGCAG 
CACCTGGCCA GGCTTGCAGA TGACTTCCAG AGAAACAAGG AGGCTCGTTT TGAGCTGGAG 
ACCTGGGGGC TGCATTTTGG ATGCCAGATG TCTCTGACTG GCCGTATTGC ACCTGCAGAA 
AAAATAATAA TGCAAGACCA CATATGTCAA CCTGCATCTG CAGCTGACTG GTCCAAGGAT 
ATACGAATTT GCAAGGTTTT AAGTGCACAG TCTTTGAATA AATGGCTGGT TATATGTAGT 
AACAGAGCTG AAAGTGTGAC AGAGAACTTT CTGAACTGCT TGAGAAGAGT TGGAGGTCCC 
ATGGGATTTA ATGTGGATTG CCCCAAAATC ATAAAAGTAC AAGAAAATCC AGCTGCATTT 
ATTCACGCTA TACAGAAGCA TGTTAAACAT GAGGTTCAGT TGGTGATGTG TATTCTGCCT 
TCTATTCAGA CCAGCTATTA TGATTCCATT AAAAAATATT TGAGCTCCGA CTGCCCAGTG 
CCCAGCCAGT GTGTGCTTGC TCAGACGTTG AATAAGCAGG GGATGATGCT GAGTGTCGCC 
ACCAAGATTG CCATGCAAAT GATCTGTAAA CTCGGGGGTG AGCTCTGGGC GGTGGAGATC 
CCGTTAAAGT CCCTGATGGT GGTCGGTATC AATGTCTGTA AAGATGCATT CAGCCAGGGA 
ACGATGGTGG TTGGATTTGT GGCCAGTATT AACCCCAGAA TCACCAGGTG GTTTTCCCGC 
TGTATCCTTC AGAGAACAAC AGCTCATGTT GCAGACTGCT TAAAAGTGTT CATGATCGGG 
GCACTGAACA GGTGGTATAC GCATAACCAG GGTTTGCCAG CACGGATAAT TGTGTACCGT 
GAAGGTGTCG GTGACGGTCA GCTCCAGACA CTCGTTGACT ATGAAGTCCC ACAACTGCTG 
AGCAGTGTGA TCGAAGCAAG TTCAAATACC AGCCCCAAAC TGTCGGTGAT TGTGGTCAGG 
AAGAAGGGCA TGCCACGATT CTTGGTGGAA ACTGGCCGAA ATCTGCAGAA CCCACCACTT 
GGCACTGTCG TGGATTCGGA AGCAACACGG CCAGAATGGT ATGACTTTTA CTTGATCAGC 
CAGATGGCCT GTCGGGGAAC CGTTAATCCC ACCTACTATA ATGTCATCTA TGATGACAAT 
GGCCTGAAGC CCGACCACAT GCAGAGACTC ACATTCAAAC TGTGTCACCT GTACTACAAC 
TGGATGGGCC TAATCAGTAT TCCAGCTCCC TGTCAGTATG CACGCAAGCT GACCTTCCTG 
GTGGCACAAA GCCTTCATAA AGAACCAAGT TTGGAATTAG CCAATTCTCT CTTCTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIWIL4 ( XM_006048173.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006048235.1 Bubalus bubalis piwi-like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Price & Availability↑

CloneID	OBu06655
Clone ID Related Accession (Same CDS sequence)	XM_006048173.1
Accession Version	XM_006048173.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	piwi-like protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784088.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGGCC GCGCCCGCGT GAGGGCGCGG GGCATGGCCC GCAGCCCCGG CGCCGCGGAG 
GTGGGGCGTG CCCGGGGGCC TCCACTTCCT CCATCTGTTG CGCTTATTGA TAATGAAGCA 
TCCTCTAGCA GTGACTGTGA GAGAACAAGC AGGATTTCAC AGAAATGTAA GTGTGGTATA 
TCTCCAAGTG ATTCTGGATG TACCTTCCAG GAAAGAGGTG GGAAAGTCAG AAGGGACATC 
ATGGATTTGG CTGTCTGTAC AAGAGAGAAA CTGGCCCATG TGAGAGACTG CAAAACAGGT 
TCCAGTGGAA TACCTGTGAA ACTGGTCACA AATCTCTTTA ATTTAGACGT GCCCCAGGAC 
TGGCAGCTAT ACCAGTATCA TGTGACATAT AGTCCGGATA TAGAGTCTAG AAGGCTGAGA 
ATTGCTCTGC TTTATAGTCA CAGGGAACTC TCCAACAAAG CAAAAGCATT CGATGGTGTC 
ATCCTTTTTC TCTCACAAAA GCTAGAAGAA AAGGTCACGG AGTTGTCAAG TGAAACCCAA 
AGAGGCGAGA CTGTAAAGAT GACTATCACT CTGACGAGAG AGCTGCCGGC AAGTTCTCCT 
GTGTGCATCC AGGTCTTCAA TATCATCTTC AAAAAGATCT TAAAAAAGTT ATCCATGTAC 
CAAATTGGAC GGAACTTCTA TAAGCCTTCA GAGCCAGTGG AAATTCCCCA GCACAAATTG 
TCTCTTTGGC CTGGGTTCGC CATTTCTGTA TCACATTTTG AAAGCAAACT CCTGTTGAGT 
GCTAATGTGA GTTACAAAAT ACTGCGGAAC GAGACAGTTC TGGAGTTCAT GACGGATCTC 
TGCCGCAAAA CTAGCGAGTC CTGCTTTACC CAGACGTGTG AGAAACAGCT GATTGGGCTC 
ATTGTCCTCA CAAGATACAA TAATAAAACC TATCGCATTG ATGACATCGA CTGGTCGGTG 
AAGCCCACAC ACACCTTCCG GAAGCGGGAC GGCGCTGAGA TCACCTACGT GGATTACTAC 
AAGCAGCAAT ACGATATTAC CTTATCTGAC CTGAACCAGC CAGTTCTTGT TAGTCTGTTG 
AAAAACAAGA AAAATGACAA CTCTGAGGCT CAGATGGCCC ACCTGATTCC CGAGCTCTGC 
TTTCTAACGG GGATGCCTGG CCAGGCGACC TCTGATTTCC AGCTGATGAA GGCTGTGGCT 
AAAGAAACTC GTCTCGATCC TTTGGTAAGG CAGCAGCACC TGGCCAGGCT TGCAGATGAC 
TTCCAGAGAA ACAAGGAGGC TCGTTTTGAG CTGGAGACCT GGGGGCTGCA TTTTGGATGC 
CAGATGTCTC TGACTGGCCG TATTGCACCT GCAGAAAAAA TAATAATGCA AGACCACATA 
TGTCAACCTG CATCTGCAGC TGACTGGTCC AAGGATATAC GAATTTGCAA GGTTTTAAGT 
GCACAGTCTT TGAATAAATG GCTGGTTATA TGTAGTAACA GAGCTGAAAG TGTGACAGAG 
AACTTTCTGA ACTGCTTGAG AAGAGTTGGA GGTCCCATGG GATTTAATGT GGATTGCCCC 
AAAATCATAA AAGTACAAGA AAATCCAGCT GCATTTATTC ACGCTATACA GAAGCATGTT 
AAACATGAGG TTCAGTTGGT GATGTGTATT CTGCCTTCTA TTCAGACCAG CTATTATGAT 
TCCATTAAAA AATATTTGAG CTCCGACTGC CCAGTGCCCA GCCAGTGTGT GCTTGCTCAG 
ACGTTGAATA AGCAGGGGAT GATGCTGAGT GTCGCCACCA AGATTGCCAT GCAAATGATC 
TGTAAACTCG GGGGTGAGCT CTGGGCGGTG GAGATCCCGT TAAAGTCCCT GATGGTGGTC 
GGTATCAATG TCTGTAAAGA TGCATTCAGC CAGGGAACGA TGGTGGTTGG ATTTGTGGCC 
AGTATTAACC CCAGAATCAC CAGGTGGTTT TCCCGCTGTA TCCTTCAGAG AACAACAGCT 
CATGTTGCAG ACTGCTTAAA AGTGTTCATG ATCGGGGCAC TGAACAGGTG GTATACGCAT 
AACCAGGGTT TGCCAGCACG GATAATTGTG TACCGTGAAG GTGTCGGTGA CGGTCAGCTC 
CAGACACTCG TTGACTATGA AGTCCCACAA CTGCTGAGCA GTGTGATCGA AGCAAGTTCA 
AATACCAGCC CCAAACTGTC GGTGATTGTG GTCAGGAAGA AGGGCATGCC ACGATTCTTG 
GTGGAAACTG GCCGAAATCT GCAGAACCCA CCACTTGGCA CTGTCGTGGA TTCGGAAGCA 
ACACGGCCAG AATGGTATGA CTTTTACTTG ATCAGCCAGA TGGCCTGTCG GGGAACCGTT 
AATCCCACCT ACTATAATGT CATCTATGAT GACAATGGCC TGAAGCCCGA CCACATGCAG 
AGACTCACAT TCAAACTGTG TCACCTGTAC TACAACTGGA TGGGCCTAAT CAGTATTCCA 
GCTCCCTGTC AGTATGCACG CAAGCTGACC TTCCTGGTGG CACAAAGCCT TCATAAAGAA 
CCAAGTTTGG AATTAGCCAA TTCTCTCTTC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006048235.1
CDS	1..2559
Translation	MSGRARVRARGMARSPGAAEVGRARGPPLPPSVALIDNEASSSSDCERTSRISQKCKCGISPSDSGCTFQERGGKVRRDIMDLAVCTREKLAHVRDCKTGSSGIPVKLVTNLFNLDVPQDWQLYQYHVTYSPDIESRRLRIALLYSHRELSNKAKAFDGVILFLSQKLEEKVTELSSETQRGETVKMTITLTRELPASSPVCIQVFNIIFKKILKKLSMYQIGRNFYKPSEPVEIPQHKLSLWPGFAISVSHFESKLLLSANVSYKILRNETVLEFMTDLCRKTSESCFTQTCEKQLIGLIVLTRYNNKTYRIDDIDWSVKPTHTFRKRDGAEITYVDYYKQQYDITLSDLNQPVLVSLLKNKKNDNSEAQMAHLIPELCFLTGMPGQATSDFQLMKAVAKETRLDPLVRQQHLARLADDFQRNKEARFELETWGLHFGCQMSLTGRIAPAEKIIMQDHICQPASAADWSKDIRICKVLSAQSLNKWLVICSNRAESVTENFLNCLRRVGGPMGFNVDCPKIIKVQENPAAFIHAIQKHVKHEVQLVMCILPSIQTSYYDSIKKYLSSDCPVPSQCVLAQTLNKQGMMLSVATKIAMQMICKLGGELWAVEIPLKSLMVVGINVCKDAFSQGTMVVGFVASINPRITRWFSRCILQRTTAHVADCLKVFMIGALNRWYTHNQGLPARIIVYREGVGDGQLQTLVDYEVPQLLSSVIEASSNTSPKLSVIVVRKKGMPRFLVETGRNLQNPPLGTVVDSEATRPEWYDFYLISQMACRGTVNPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWMGLISIPAPCQYARKLTFLVAQSLHKEPSLELANSLFYL

Target ORF information:

RefSeq Version	XM_006048173.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis piwi-like RNA-mediated gene silencing 4 (PIWIL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006048173.1

ORF Insert Sequence:

ATGAGCGGCC GCGCCCGCGT GAGGGCGCGG GGCATGGCCC GCAGCCCCGG CGCCGCGGAG 
GTGGGGCGTG CCCGGGGGCC TCCACTTCCT CCATCTGTTG CGCTTATTGA TAATGAAGCA 
TCCTCTAGCA GTGACTGTGA GAGAACAAGC AGGATTTCAC AGAAATGTAA GTGTGGTATA 
TCTCCAAGTG ATTCTGGATG TACCTTCCAG GAAAGAGGTG GGAAAGTCAG AAGGGACATC 
ATGGATTTGG CTGTCTGTAC AAGAGAGAAA CTGGCCCATG TGAGAGACTG CAAAACAGGT 
TCCAGTGGAA TACCTGTGAA ACTGGTCACA AATCTCTTTA ATTTAGACGT GCCCCAGGAC 
TGGCAGCTAT ACCAGTATCA TGTGACATAT AGTCCGGATA TAGAGTCTAG AAGGCTGAGA 
ATTGCTCTGC TTTATAGTCA CAGGGAACTC TCCAACAAAG CAAAAGCATT CGATGGTGTC 
ATCCTTTTTC TCTCACAAAA GCTAGAAGAA AAGGTCACGG AGTTGTCAAG TGAAACCCAA 
AGAGGCGAGA CTGTAAAGAT GACTATCACT CTGACGAGAG AGCTGCCGGC AAGTTCTCCT 
GTGTGCATCC AGGTCTTCAA TATCATCTTC AAAAAGATCT TAAAAAAGTT ATCCATGTAC 
CAAATTGGAC GGAACTTCTA TAAGCCTTCA GAGCCAGTGG AAATTCCCCA GCACAAATTG 
TCTCTTTGGC CTGGGTTCGC CATTTCTGTA TCACATTTTG AAAGCAAACT CCTGTTGAGT 
GCTAATGTGA GTTACAAAAT ACTGCGGAAC GAGACAGTTC TGGAGTTCAT GACGGATCTC 
TGCCGCAAAA CTAGCGAGTC CTGCTTTACC CAGACGTGTG AGAAACAGCT GATTGGGCTC 
ATTGTCCTCA CAAGATACAA TAATAAAACC TATCGCATTG ATGACATCGA CTGGTCGGTG 
AAGCCCACAC ACACCTTCCG GAAGCGGGAC GGCGCTGAGA TCACCTACGT GGATTACTAC 
AAGCAGCAAT ACGATATTAC CTTATCTGAC CTGAACCAGC CAGTTCTTGT TAGTCTGTTG 
AAAAACAAGA AAAATGACAA CTCTGAGGCT CAGATGGCCC ACCTGATTCC CGAGCTCTGC 
TTTCTAACGG GGATGCCTGG CCAGGCGACC TCTGATTTCC AGCTGATGAA GGCTGTGGCT 
AAAGAAACTC GTCTCGATCC TTTGGTAAGG CAGCAGCACC TGGCCAGGCT TGCAGATGAC 
TTCCAGAGAA ACAAGGAGGC TCGTTTTGAG CTGGAGACCT GGGGGCTGCA TTTTGGATGC 
CAGATGTCTC TGACTGGCCG TATTGCACCT GCAGAAAAAA TAATAATGCA AGACCACATA 
TGTCAACCTG CATCTGCAGC TGACTGGTCC AAGGATATAC GAATTTGCAA GGTTTTAAGT 
GCACAGTCTT TGAATAAATG GCTGGTTATA TGTAGTAACA GAGCTGAAAG TGTGACAGAG 
AACTTTCTGA ACTGCTTGAG AAGAGTTGGA GGTCCCATGG GATTTAATGT GGATTGCCCC 
AAAATCATAA AAGTACAAGA AAATCCAGCT GCATTTATTC ACGCTATACA GAAGCATGTT 
AAACATGAGG TTCAGTTGGT GATGTGTATT CTGCCTTCTA TTCAGACCAG CTATTATGAT 
TCCATTAAAA AATATTTGAG CTCCGACTGC CCAGTGCCCA GCCAGTGTGT GCTTGCTCAG 
ACGTTGAATA AGCAGGGGAT GATGCTGAGT GTCGCCACCA AGATTGCCAT GCAAATGATC 
TGTAAACTCG GGGGTGAGCT CTGGGCGGTG GAGATCCCGT TAAAGTCCCT GATGGTGGTC 
GGTATCAATG TCTGTAAAGA TGCATTCAGC CAGGGAACGA TGGTGGTTGG ATTTGTGGCC 
AGTATTAACC CCAGAATCAC CAGGTGGTTT TCCCGCTGTA TCCTTCAGAG AACAACAGCT 
CATGTTGCAG ACTGCTTAAA AGTGTTCATG ATCGGGGCAC TGAACAGGTG GTATACGCAT 
AACCAGGGTT TGCCAGCACG GATAATTGTG TACCGTGAAG GTGTCGGTGA CGGTCAGCTC 
CAGACACTCG TTGACTATGA AGTCCCACAA CTGCTGAGCA GTGTGATCGA AGCAAGTTCA 
AATACCAGCC CCAAACTGTC GGTGATTGTG GTCAGGAAGA AGGGCATGCC ACGATTCTTG 
GTGGAAACTG GCCGAAATCT GCAGAACCCA CCACTTGGCA CTGTCGTGGA TTCGGAAGCA 
ACACGGCCAG AATGGTATGA CTTTTACTTG ATCAGCCAGA TGGCCTGTCG GGGAACCGTT 
AATCCCACCT ACTATAATGT CATCTATGAT GACAATGGCC TGAAGCCCGA CCACATGCAG 
AGACTCACAT TCAAACTGTG TCACCTGTAC TACAACTGGA TGGGCCTAAT CAGTATTCCA 
GCTCCCTGTC AGTATGCACG CAAGCTGACC TTCCTGGTGG CACAAAGCCT TCATAAAGAA 
CCAAGTTTGG AATTAGCCAA TTCTCTCTTC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PIWIL4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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