RASAL2 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006071063.1	XP_006071125.1	ras GTPase-activating protein nGAP isoform X3
XM_025285576.1	XP_025141361.1	ras GTPase-activating protein nGAP isoform X3
XM_025285581.1	XP_025141366.1	ras GTPase-activating protein nGAP isoform X7
XM_025285582.1	XP_025141367.1	ras GTPase-activating protein nGAP isoform X8
XM_006071062.1	XP_006071124.1	ras GTPase-activating protein nGAP isoform X2
XM_025285574.1	XP_025141359.1	ras GTPase-activating protein nGAP isoform X1
XM_025285575.1	XP_025141360.1	ras GTPase-activating protein nGAP isoform X2
XM_025285580.1	XP_025141365.1	ras GTPase-activating protein nGAP isoform X6
XM_025285578.1	XP_025141363.1	ras GTPase-activating protein nGAP isoform X5
XM_006071061.1	XP_006071123.1	ras GTPase-activating protein nGAP isoform X1
XM_025285577.1	XP_025141362.1	ras GTPase-activating protein nGAP isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu25563	XM_006071061.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu25564	XM_006071062.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu25565	XM_006071063.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu66027	XM_025285574.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66028	XM_025285575.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66029	XM_025285576.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu66030	XM_025285577.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu66031	XM_025285578.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu66032	XM_025285580.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu66033	XM_025285581.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu66034	XM_025285582.1 Latest version!	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

RASAL2 ( XM_006071061.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006071123.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu25563
Clone ID Related Accession (Same CDS sequence)	XM_006071061.1
Accession Version	XM_006071061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785419.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCTTCTC TTCGCAATAC CGATGACAGG 
TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC 
AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TATCGGTAAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT 
CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATCT TCTGGGGTGA ACATTTTGAG 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA 
AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT 
GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC 
ATTCTTCAAA GCACGGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG 
GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGCT GGTGGGACAA CAGTATCTTC ACGACGCACT GGGGGAGTTC 
ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC 
GGCGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCTTGGAAG 
CACCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGTGAGC GGCTCATCAG CGCCTCACTG 
TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG 
GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTG 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACTATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA 
ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT 
AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT 
TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA 
TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG 
GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCG 
GCCCAAGCGG AGCATCCGTC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG 
CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCTTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG 
GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC 
AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG 
AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC 
GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG 
CTGGGCGCCC GAGCCAAAGC CCCGCCATCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC 
GGGAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG 
TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTAC CCAAAGTGAG AGCCATCCAG 
AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG 
AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG 
CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG 
GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG 
CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGAAAAA CGGATCGTGT CCCTGGATTC GGCCAACACC 
AGACTGATGA GCGCGCTGAC CCAGGTGAAG GAACGGTACA GCATGCAGGT CCGAAATGGC 
ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGAATT CAAAAACAGC 
AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006071123.1
CDS	713..4141
Translation	MGSSCDKEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSGELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_006071061.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006071061.1

ORF Insert Sequence:

ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCTTCTC TTCGCAATAC CGATGACAGG 
TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC 
AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TATCGGTAAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT 
CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATCT TCTGGGGTGA ACATTTTGAG 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA 
AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT 
GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC 
ATTCTTCAAA GCACGGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG 
GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGCT GGTGGGACAA CAGTATCTTC ACGACGCACT GGGGGAGTTC 
ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC 
GGCGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCTTGGAAG 
CACCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGTGAGC GGCTCATCAG CGCCTCACTG 
TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG 
GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTG 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACTATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA 
ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT 
AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT 
TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA 
TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG 
GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCG 
GCCCAAGCGG AGCATCCGTC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG 
CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCTTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG 
GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC 
AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG 
AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC 
GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG 
CTGGGCGCCC GAGCCAAAGC CCCGCCATCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC 
GGGAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG 
TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTAC CCAAAGTGAG AGCCATCCAG 
AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG 
AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG 
CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG 
GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG 
CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGAAAAA CGGATCGTGT CCCTGGATTC GGCCAACACC 
AGACTGATGA GCGCGCTGAC CCAGGTGAAG GAACGGTACA GCATGCAGGT CCGAAATGGC 
ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGAATT CAAAAACAGC 
AGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_006071062.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006071124.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu25564
Clone ID Related Accession (Same CDS sequence)	XM_006071062.1
Accession Version	XM_006071062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785419.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACCGAAACA CGAGCTTTCG GCTTCCTTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG 
CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG 
GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTATCGGTAA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT 
AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGACAATATC TTCTGGGGTG AACATTTTGA GTTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA 
CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA 
AGCACGGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT 
GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGC TGGTGGGACA ACAGTATCTT CACGACGCAC TGGGGGAGTT CATCAAAGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CGGCGAACTT 
CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCTTGGAA GCACCAGTGC 
CTGAACCGCG GCAAGCAGGA CATCAGTGAG CGGCTCATCA GCGCCTCACT GTTTCTCCGT 
TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT 
GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT GGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACTAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA 
AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA 
CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG 
GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC 
CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC GGCCCAAGCG 
GAGCATCCGT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA 
ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCC 
TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG 
GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC 
TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG 
AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG 
CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC 
CGAGCCAAAG CCCCGCCATC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG 
TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCGGTA CCCAAAGTGA GAGCCATCCA GAGGCAGCAG 
ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG 
GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006071124.1
CDS	114..3533
Translation	MQTPEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSGELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_006071062.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006071062.1

ORF Insert Sequence:

ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACCGAAACA CGAGCTTTCG GCTTCCTTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG 
CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG 
GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTATCGGTAA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT 
AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGACAATATC TTCTGGGGTG AACATTTTGA GTTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA 
CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA 
AGCACGGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT 
GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGC TGGTGGGACA ACAGTATCTT CACGACGCAC TGGGGGAGTT CATCAAAGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CGGCGAACTT 
CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCTTGGAA GCACCAGTGC 
CTGAACCGCG GCAAGCAGGA CATCAGTGAG CGGCTCATCA GCGCCTCACT GTTTCTCCGT 
TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT 
GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT GGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACTAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA 
AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA 
CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG 
GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC 
CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC GGCCCAAGCG 
GAGCATCCGT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA 
ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCC 
TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG 
GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC 
TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG 
AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG 
CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC 
CGAGCCAAAG CCCCGCCATC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG 
TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCGGTA CCCAAAGTGA GAGCCATCCA GAGGCAGCAG 
ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG 
GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGAAAA ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG 
AGCGCGCTGA CCCAGGTGAA GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC 
ACCAACCCCA CCAAGCTTTC CATCACGGAG AATGGTGAAT TCAAAAACAG CAGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_006071063.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006071125.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu25565
Clone ID Related Accession (Same CDS sequence)	XM_006071063.1
Accession Version	XM_006071063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785419.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCTTCTC TTCGCAATAC CGATGACAGG 
TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC 
AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TATCGGTAAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT 
CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATCT TCTGGGGTGA ACATTTTGAG 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA 
AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT 
GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC 
ATTCTTCAAA GCACGGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG 
GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGCT GGTGGGACAA CAGTATCTTC ACGACGCACT GGGGGAGTTC 
ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC 
GGCGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCTTGGAAG 
CACCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGTGAGC GGCTCATCAG CGCCTCACTG 
TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG 
GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTG 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACTATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGCAA CCGTGGCAAA ATTGGGACCT 
CTCCCTCGTG TTCTTGCTGA TATTACCAAA TCTCTGACTA ATCCTACACC AATACAACAG 
CAACTGAGAC GCTTCACTGA GCATAACTCC AGTCCAAATG TCAGTGGAAG CCTCTCCTCT 
GGGCTGCAGA AAATATTTGA AGACCCTACT GACAGTGATT TGCACAAACT AAAATCCCCA 
AGCCAGGACA ACACAGACAG TTATTTCAGA GGGAAGACAT TATTGCTGGT CCAGCAAGCC 
TCCTCCCAGA GCATGACTTA CTCGGAAAAG GATGAAAAGG AAAGTAGCCT CCCCAATGGC 
CGCAGCATCT CCCTCATGGA CCTTCAGGAG ACGCACGCGG CCCAAGCGGA GCATCCGTCT 
GTGATACTCG ACGTGCCCAT GCGCTTGACC GGAAGCCAGC TCTCCATAAC TCAGGTGGCC 
AGCATCAAGC AACTGCGGGA GACCCAGAGC ACCCCCCAGA GTGCACCCCA AGTGCGAAGG 
CCCCTGCACC CAGCCTTGAA CCAGCCAGGC AGCCTCCAGC CCTTGTCCTT CCAGAACCCT 
GTCTATCACC TCAGTAACCC CATTCCTGCA ATGCCAAAGG CCTCTGTGGA CTCCAGTTTG 
GAGAACCTAA GCACTGCCAG TTCCAGGAGC CAAAGTAACA GTGAAGACTT CAAACTCAGC 
GGACCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAACGGA GCACGCAGAG TGAGGACTTC 
TCCAGGCGCC ACACCATGCC CGACAGACAC ATACCTCTCG CCCTGCCGCG GCAGAACAGC 
ACGGGGCAGA CCCAGCTCCG GAAGATGGAC CCGGCGGGGC TGGGCGCCCG AGCCAAAGCC 
CCGCCATCCC TGCCGCACAG CGCCTCCCTG CGGAGCACCG GGAGCATGTC GGTGGCATCG 
GCGGCTCTGG TGGCCGAGCC CCTGCAGAAC GGGAGCCGGT CCCGACAGCA GTCCTCTTCC 
TCCAGAGAGA GCCCGGTACC CAAAGTGAGA GCCATCCAGA GGCAGCAGAC CCAGCAGGTT 
CAGTCACCTG TGGACTCCGC CACAATGTCC CCAGTCGAGA GGACAGCAGC CTGGGTTCTG 
AACAATGGGC AGTATGAAGA GGACGTGGAA GAGACTGAGC AAAATCAGGA CGAAGCCAAG 
CATGCTGAGA AGTACGAACA GGAAATCACC AAACTGAAGG AGCGCCTGCG AGTGTCCAGC 
CGGCGACTCG AGGAGTATGA GCGCCGGTTG CTAGTGCAGG AGCAGCAGAT GCAGAAGCTG 
CTGCTGGAGT ACAAGGCCCG GCTGGAGGAC AGCGAGGAGC GGCTGCGCAG GCAGCAGGAG 
GAGAAGGACA GCCAGATGAA GAGCATCATC AGCAGGCTAA TGGCTGTGGA AGAGGAACTG 
AAGAAGGATC ATGCTGAGAT GCAGGCAGTT ATTGATGCAA AGCAAAAAAT AATCGATGCA 
CAGGTCATAA ATGGAGATGA GATTATGCAA ACAGGAAAAA CGGATCGTGT CCCTGGATTC 
GGCCAACACC AGACTGATGA GCGCGCTGAC CCAGGTGAAG GAACGGTACA GCATGCAGGT 
CCGAAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006071125.1
CDS	713..4129
Translation	MGSSCDKEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSGELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIMQTGKTDRVPGFGQHQTDERADPGEGTVQHAGPKWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_006071063.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006071063.1

ORF Insert Sequence:

ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCTTCTC TTCGCAATAC CGATGACAGG 
TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC 
AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TATCGGTAAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT 
CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATCT TCTGGGGTGA ACATTTTGAG 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA 
AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT 
GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC 
ATTCTTCAAA GCACGGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG 
GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGCT GGTGGGACAA CAGTATCTTC ACGACGCACT GGGGGAGTTC 
ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC 
GGCGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCTTGGAAG 
CACCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGTGAGC GGCTCATCAG CGCCTCACTG 
TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG 
GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTG 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACTATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGCAA CCGTGGCAAA ATTGGGACCT 
CTCCCTCGTG TTCTTGCTGA TATTACCAAA TCTCTGACTA ATCCTACACC AATACAACAG 
CAACTGAGAC GCTTCACTGA GCATAACTCC AGTCCAAATG TCAGTGGAAG CCTCTCCTCT 
GGGCTGCAGA AAATATTTGA AGACCCTACT GACAGTGATT TGCACAAACT AAAATCCCCA 
AGCCAGGACA ACACAGACAG TTATTTCAGA GGGAAGACAT TATTGCTGGT CCAGCAAGCC 
TCCTCCCAGA GCATGACTTA CTCGGAAAAG GATGAAAAGG AAAGTAGCCT CCCCAATGGC 
CGCAGCATCT CCCTCATGGA CCTTCAGGAG ACGCACGCGG CCCAAGCGGA GCATCCGTCT 
GTGATACTCG ACGTGCCCAT GCGCTTGACC GGAAGCCAGC TCTCCATAAC TCAGGTGGCC 
AGCATCAAGC AACTGCGGGA GACCCAGAGC ACCCCCCAGA GTGCACCCCA AGTGCGAAGG 
CCCCTGCACC CAGCCTTGAA CCAGCCAGGC AGCCTCCAGC CCTTGTCCTT CCAGAACCCT 
GTCTATCACC TCAGTAACCC CATTCCTGCA ATGCCAAAGG CCTCTGTGGA CTCCAGTTTG 
GAGAACCTAA GCACTGCCAG TTCCAGGAGC CAAAGTAACA GTGAAGACTT CAAACTCAGC 
GGACCCAGCA ACAGCAGCAT GGAGGACTTC ACCAAACGGA GCACGCAGAG TGAGGACTTC 
TCCAGGCGCC ACACCATGCC CGACAGACAC ATACCTCTCG CCCTGCCGCG GCAGAACAGC 
ACGGGGCAGA CCCAGCTCCG GAAGATGGAC CCGGCGGGGC TGGGCGCCCG AGCCAAAGCC 
CCGCCATCCC TGCCGCACAG CGCCTCCCTG CGGAGCACCG GGAGCATGTC GGTGGCATCG 
GCGGCTCTGG TGGCCGAGCC CCTGCAGAAC GGGAGCCGGT CCCGACAGCA GTCCTCTTCC 
TCCAGAGAGA GCCCGGTACC CAAAGTGAGA GCCATCCAGA GGCAGCAGAC CCAGCAGGTT 
CAGTCACCTG TGGACTCCGC CACAATGTCC CCAGTCGAGA GGACAGCAGC CTGGGTTCTG 
AACAATGGGC AGTATGAAGA GGACGTGGAA GAGACTGAGC AAAATCAGGA CGAAGCCAAG 
CATGCTGAGA AGTACGAACA GGAAATCACC AAACTGAAGG AGCGCCTGCG AGTGTCCAGC 
CGGCGACTCG AGGAGTATGA GCGCCGGTTG CTAGTGCAGG AGCAGCAGAT GCAGAAGCTG 
CTGCTGGAGT ACAAGGCCCG GCTGGAGGAC AGCGAGGAGC GGCTGCGCAG GCAGCAGGAG 
GAGAAGGACA GCCAGATGAA GAGCATCATC AGCAGGCTAA TGGCTGTGGA AGAGGAACTG 
AAGAAGGATC ATGCTGAGAT GCAGGCAGTT ATTGATGCAA AGCAAAAAAT AATCGATGCA 
CAGGTCATAA ATGGAGATGA GATTATGCAA ACAGGAAAAA CGGATCGTGT CCCTGGATTC 
GGCCAACACC AGACTGATGA GCGCGCTGAC CCAGGTGAAG GAACGGTACA GCATGCAGGT 
CCGAAATGGC ATCTCCCCCA CCAACCCCAC CAAGCTTTCC ATCACGGAGA ATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285574.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141359.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu66027
Clone ID Related Accession (Same CDS sequence)	XM_025285574.1
Accession Version	XM_025285574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAT GGAAGATGAG CATCTTTAAG AGACAAACAT CACCGTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAACAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG 
GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCA 
GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAGTGAAGT CCTTGGGACA 
TCAATGGAGA AGCAGAACCT GGTAAACACT CGGTCACTTC GGGTTCCATT CAGCAGATCT 
CTTTCAGAGC CAGAACCCTG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG 
GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC 
AATGGCTTCT CCAGGATGCT GAATCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA 
GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACG 
ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG 
GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA 
TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG 
GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG 
ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA 
CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG 
CTTGACCGAA ACACGAGCTT TCGGCTTCCT TCTCTTCGCA ATACCGATGA CAGGTCTCGT 
GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC 
GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTATCGG TAAAGCCACT CCATAGCAGC 
ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT 
TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA 
AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG 
GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT 
CGTACAACCA GCAAGACCAA AGCAGACAAT ATCTTCTGGG GTGAACATTT TGAGTTCTAC 
AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG 
AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT 
CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA 
GGAGGACCTT CTATTCGGAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG 
CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT 
GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT 
CAAAGCACGG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT 
TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG 
GAATACCTCA AGCTGGTGGG ACAACAGTAT CTTCACGACG CACTGGGGGA GTTCATCAAA 
GCTTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA 
CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC 
AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCTTG GAAGCACCAG 
TGCCTGAACC GCGGCAAGCA GGACATCAGT GAGCGGCTCA TCAGCGCCTC ACTGTTTCTC 
CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT 
GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC 
TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTGGAACAT 
GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC TATCTCAAAC 
ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA 
CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG 
GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC 
AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG 
CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT 
AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCGGCCCAA 
GCGGAGCATC CGTCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC 
ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT 
GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA 
GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG 
CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG 
CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC 
GCCCGAGCCA AAGCCCCGCC ATCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC 
ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTACCCAAAG TGAGAGCCAT CCAGAGGCAG 
CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT 
CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT 
CGTGTCCCTG GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG 
GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC 
GGAGAATGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141359.1
CDS	314..4585
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAHSEIGTSLKSYPWRGPVKSKRKKNEQAVSSLGKPLGAYRWQTHPSLRLKGDKKEQLENNKGDLDEPQQQAVLPKMVGDNISSSGSEVLGTSMEKQNLVNTRSLRVPFSRSLSEPEPCSSVRFVRRWPTSVGSVESEQQGYFIRGIFSTLSNGFSRMLNLKGGSTSEPVREDVKGPPTHRLSCGQSPYTDTTTWERKYCILTDSQLVLLNKEKEIPVDGGQEQQADSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIMQTGKTDRVPGFGQHQTDERADPGEGTVQHAGPKWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_025285574.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285574.1

ORF Insert Sequence:

ATGGAGTCAT GGAAGATGAG CATCTTTAAG AGACAAACAT CACCGTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAACAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG 
GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCA 
GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAGTGAAGT CCTTGGGACA 
TCAATGGAGA AGCAGAACCT GGTAAACACT CGGTCACTTC GGGTTCCATT CAGCAGATCT 
CTTTCAGAGC CAGAACCCTG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG 
GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC 
AATGGCTTCT CCAGGATGCT GAATCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA 
GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACG 
ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG 
GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA 
TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG 
GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG 
ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA 
CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG 
CTTGACCGAA ACACGAGCTT TCGGCTTCCT TCTCTTCGCA ATACCGATGA CAGGTCTCGT 
GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC 
GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTATCGG TAAAGCCACT CCATAGCAGC 
ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT 
TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA 
AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG 
GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT 
CGTACAACCA GCAAGACCAA AGCAGACAAT ATCTTCTGGG GTGAACATTT TGAGTTCTAC 
AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG 
AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT 
CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA 
GGAGGACCTT CTATTCGGAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG 
CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT 
GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT 
CAAAGCACGG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT 
TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG 
GAATACCTCA AGCTGGTGGG ACAACAGTAT CTTCACGACG CACTGGGGGA GTTCATCAAA 
GCTTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA 
CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC 
AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCTTG GAAGCACCAG 
TGCCTGAACC GCGGCAAGCA GGACATCAGT GAGCGGCTCA TCAGCGCCTC ACTGTTTCTC 
CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT 
GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC 
TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTGGAACAT 
GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC TATCTCAAAC 
ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA 
CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG 
GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC 
AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG 
CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT 
AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCGGCCCAA 
GCGGAGCATC CGTCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC 
ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT 
GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA 
GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG 
CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG 
CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC 
GCCCGAGCCA AAGCCCCGCC ATCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC 
ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTACCCAAAG TGAGAGCCAT CCAGAGGCAG 
CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT 
CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGT CATAAATGGA GATGAGATTA TGCAAACAGG AAAAACGGAT 
CGTGTCCCTG GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG 
GTACAGCATG CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC 
GGAGAATGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285575.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141360.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

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CloneID	OBu66028
Clone ID Related Accession (Same CDS sequence)	XM_025285575.1
Accession Version	XM_025285575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAT GGAAGATGAG CATCTTTAAG AGACAAACAT CACCGTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAACAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG 
GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCA 
GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAGTGAAGT CCTTGGGACA 
TCAATGGAGA AGCAGAACCT GGTAAACACT CGGTCACTTC GGGTTCCATT CAGCAGATCT 
CTTTCAGAGC CAGAACCCTG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG 
GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC 
AATGGCTTCT CCAGGATGCT GAATCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA 
GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACG 
ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG 
GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA 
TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG 
GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG 
ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA 
CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG 
CTTGACCGAA ACACGAGCTT TCGGCTTCCT TCTCTTCGCA ATACCGATGA CAGGTCTCGT 
GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC 
GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTATCGG TAAAGCCACT CCATAGCAGC 
ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT 
TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA 
AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG 
GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT 
CGTACAACCA GCAAGACCAA AGCAGACAAT ATCTTCTGGG GTGAACATTT TGAGTTCTAC 
AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG 
AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT 
CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA 
GGAGGACCTT CTATTCGGAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG 
CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT 
GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT 
CAAAGCACGG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT 
TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG 
GAATACCTCA AGCTGGTGGG ACAACAGTAT CTTCACGACG CACTGGGGGA GTTCATCAAA 
GCTTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA 
CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC 
AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCTTG GAAGCACCAG 
TGCCTGAACC GCGGCAAGCA GGACATCAGT GAGCGGCTCA TCAGCGCCTC ACTGTTTCTC 
CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT 
GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC 
TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTGGAACAT 
GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC TATCTCAAAC 
ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA 
CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG 
GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC 
AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG 
CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT 
AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCGGCCCAA 
GCGGAGCATC CGTCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC 
ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT 
GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA 
GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG 
CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG 
CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC 
GCCCGAGCCA AAGCCCCGCC ATCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC 
ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTACCCAAAG TGAGAGCCAT CCAGAGGCAG 
CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT 
CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141360.1
CDS	310..4572
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAHSEIGTSLKSYPWRGPVKSKRKKNEQAVSSLGKPLGAYRWQTHPSLRLKGDKKEQLENNKGDLDEPQQQAVLPKMVGDNISSSGSEVLGTSMEKQNLVNTRSLRVPFSRSLSEPEPCSSVRFVRRWPTSVGSVESEQQGYFIRGIFSTLSNGFSRMLNLKGGSTSEPVREDVKGPPTHRLSCGQSPYTDTTTWERKYCILTDSQLVLLNKEKEIPVDGGQEQQADSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_025285575.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285575.1

ORF Insert Sequence:

ATGGAGTCAT GGAAGATGAG CATCTTTAAG AGACAAACAT CACCGTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAACAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG 
GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCA 
GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAGTGAAGT CCTTGGGACA 
TCAATGGAGA AGCAGAACCT GGTAAACACT CGGTCACTTC GGGTTCCATT CAGCAGATCT 
CTTTCAGAGC CAGAACCCTG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG 
GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC 
AATGGCTTCT CCAGGATGCT GAATCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA 
GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACG 
ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG 
GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA 
TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG 
GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG 
ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA 
CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG 
CTTGACCGAA ACACGAGCTT TCGGCTTCCT TCTCTTCGCA ATACCGATGA CAGGTCTCGT 
GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC 
GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTATCGG TAAAGCCACT CCATAGCAGC 
ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT 
TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA 
AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG 
GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT 
CGTACAACCA GCAAGACCAA AGCAGACAAT ATCTTCTGGG GTGAACATTT TGAGTTCTAC 
AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG 
AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT 
CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA 
GGAGGACCTT CTATTCGGAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG 
CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT 
GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT 
CAAAGCACGG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT 
TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG 
GAATACCTCA AGCTGGTGGG ACAACAGTAT CTTCACGACG CACTGGGGGA GTTCATCAAA 
GCTTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA 
CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC 
AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCTTG GAAGCACCAG 
TGCCTGAACC GCGGCAAGCA GGACATCAGT GAGCGGCTCA TCAGCGCCTC ACTGTTTCTC 
CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT 
GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC 
TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTGGAACAT 
GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC TATCTCAAAC 
ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA 
CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GGTGAAAATT CCTTCCTACA GGCAACCGTG 
GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC 
AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG 
CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT 
AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCGGCCCAA 
GCGGAGCATC CGTCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC 
ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT 
GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA 
GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG 
CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG 
CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC 
GCCCGAGCCA AAGCCCCGCC ATCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC 
ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTACCCAAAG TGAGAGCCAT CCAGAGGCAG 
CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT 
CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285576.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141361.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

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CloneID	OBu66029
Clone ID Related Accession (Same CDS sequence)	XM_025285576.1
Accession Version	XM_025285576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCAT GGAAGATGAG CATCTTTAAG AGACAAACAT CACCGTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAACAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG 
GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCA 
GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAGTGAAGT CCTTGGGACA 
TCAATGGAGA AGCAGAACCT GGTAAACACT CGGTCACTTC GGGTTCCATT CAGCAGATCT 
CTTTCAGAGC CAGAACCCTG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG 
GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC 
AATGGCTTCT CCAGGATGCT GAATCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA 
GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACG 
ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG 
GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA 
TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG 
GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG 
ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA 
CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG 
CTTGACCGAA ACACGAGCTT TCGGCTTCCT TCTCTTCGCA ATACCGATGA CAGGTCTCGT 
GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC 
GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTATCGG TAAAGCCACT CCATAGCAGC 
ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT 
TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA 
AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG 
GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT 
CGTACAACCA GCAAGACCAA AGCAGACAAT ATCTTCTGGG GTGAACATTT TGAGTTCTAC 
AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG 
AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT 
CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA 
GGAGGACCTT CTATTCGGAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG 
CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT 
GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT 
CAAAGCACGG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT 
TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG 
GAATACCTCA AGCTGGTGGG ACAACAGTAT CTTCACGACG CACTGGGGGA GTTCATCAAA 
GCTTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA 
CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC 
AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCTTG GAAGCACCAG 
TGCCTGAACC GCGGCAAGCA GGACATCAGT GAGCGGCTCA TCAGCGCCTC ACTGTTTCTC 
CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT 
GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC 
TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTGGAACAT 
GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC TATCTCAAAC 
ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA 
CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT 
CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG 
AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG 
CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG 
GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC 
CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC 
ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCGGCCCAAG CGGAGCATCC GTCTGTGATA 
CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC 
AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG 
CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCTTGT CCTTCCAGAA CCCTGTCTAT 
CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC 
CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC 
AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG 
CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG 
CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCA 
TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCGGCGGCT 
CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA 
GAGAGCCCGG TACCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA 
CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT TCTGAACAAT 
GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT 
GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGAGTGTC CAGCCGGCGA 
CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG 
GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG 
GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG 
GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC 
ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA 
CACCAGACTG ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA 
TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141361.1
CDS	316..4566
Translation	MESWKMSIFKRQTSPCPPSYRRSLSEPAYAHSEIGTSLKSYPWRGPVKSKRKKNEQAVSSLGKPLGAYRWQTHPSLRLKGDKKEQLENNKGDLDEPQQQAVLPKMVGDNISSSGSEVLGTSMEKQNLVNTRSLRVPFSRSLSEPEPCSSVRFVRRWPTSVGSVESEQQGYFIRGIFSTLSNGFSRMLNLKGGSTSEPVREDVKGPPTHRLSCGQSPYTDTTTWERKYCILTDSQLVLLNKEKEIPVDGGQEQQADSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIMQTGKTDRVPGFGQHQTDERADPGEGTVQHAGPKWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_025285576.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285576.1

ORF Insert Sequence:

ATGGAGTCAT GGAAGATGAG CATCTTTAAG AGACAAACAT CACCGTGTCC TCCAAGCTAT 
CGAAGATCTT TGTCAGAACC TGCTTATGCA CACTCAGAGA TAGGAACAAG CTTAAAGAGT 
TATCCATGGC GTGGTCCAGT GAAAAGCAAG AGGAAGAAAA ATGAACAGGC TGTTTCCAGT 
CTAGGAAAGC CACTGGGTGC TTATCGGTGG CAAACACACC CCAGCTTAAG GTTGAAAGGG 
GACAAAAAGG AGCAATTGGA AAATAATAAA GGAGACTTGG ATGAACCACA ACAACAAGCA 
GTCCTGCCAA AGATGGTTGG GGATAATATT TCTTCCTCTG GCAGTGAAGT CCTTGGGACA 
TCAATGGAGA AGCAGAACCT GGTAAACACT CGGTCACTTC GGGTTCCATT CAGCAGATCT 
CTTTCAGAGC CAGAACCCTG CTCCAGTGTC AGATTTGTTA GACGGTGGCC AACTTCAGTG 
GGGTCAGTGG AGTCTGAGCA GCAGGGATAT TTCATCCGTG GCATTTTCTC TACTCTTTCC 
AATGGCTTCT CCAGGATGCT GAATCTAAAA GGAGGGTCAA CCAGTGAGCC AGTAAGAGAA 
GATGTGAAAG GGCCACCTAC CCACCGTCTG TCTTGTGGCC AGTCGCCCTA CACCGACACG 
ACAACATGGG AGCGGAAGTA CTGCATCCTC ACAGACAGCC AGCTGGTGTT GCTCAACAAG 
GAGAAGGAGA TACCTGTGGA CGGAGGACAA GAACAGCAGG CGGATTCCAC CAAAGGGCGA 
TGCCTGCGGA GAACTGTCAG TGTTCCTTCC GAGGGTCAGT TTCCTGAATA CCCACCAGAG 
GGCGCCACTA AACTGGAGGT GCCAGCAGAA AGGTCCCCAC GCAGACGGAG TATCTCAGGG 
ACCACTACAT CTGAGAAACC CAACTCTATG GACACTGCGA ATACCTCACC CTTCAAAGTA 
CCAGGGTTCT TCAGCAAGCG CCTAAAAGGC TCCATCAAGA GGACCAAAAG CCAATCAAAG 
CTTGACCGAA ACACGAGCTT TCGGCTTCCT TCTCTTCGCA ATACCGATGA CAGGTCTCGT 
GGGCTGCCTA AACTAAAAGA GTCCCGTTCT CATGAATCTT TGCTGAGCCC ATGCAGTGCC 
GTGGAATGTC TGGATCTCGG TAGAGGGGAG CCTGTATCGG TAAAGCCACT CCATAGCAGC 
ATCCTTGGAC AAGACTTCTG CTTTGAGGTT ACCTACTTAA GTGGAAGTAA ATGCTTCAGT 
TGTAACTCTG CTTCAGAGAG AGATAAGTGG ATGGAAAACC TGCGCAGGAC AGTTCAACCA 
AATAAGGACA ATTGTAGACG AGCTGAAAAT GTTCTCCGTT TATGGATTAT TGAAGCCAAG 
GACCTTGCCC CTAAAAAGAA ATATTTCTGT GAACTGTGCC TTGATGATAC CCTCTTTGCT 
CGTACAACCA GCAAGACCAA AGCAGACAAT ATCTTCTGGG GTGAACATTT TGAGTTCTAC 
AGCCTTCCAC CTCTTCATAG CATCACAGTT CACATTTATA AAGATGTGGA AAAAAAGAAG 
AAAAAAGACA AGAATAATTA TGTAGGGCTA GTCAACATCC CCACTGCCAG TGTGACTGGT 
CGCCAGTTTG TAGAAAAGTG GTACCCAGTG AGTACACCTA CACCCAACAA AGGAAAGACA 
GGAGGACCTT CTATTCGGAT TAAATCACGT TTCCAAACCA TCACCATTCT GCCTATGGAG 
CAATACAAAG AGTTTGCAGA ATTTGTCACC AGCAACTATA CCATGTTATG TTCTGTTCTT 
GAACCAGTAA TCAGTGTGAG AAATAAAGAA GAGCTAGCCT GTGCCTTAGT GCACATTCTT 
CAAAGCACGG GAAGGGCCAA GGATTTTCTG ACGGACTTGG TGATGTCTGA GGTGGATCGT 
TGTGGAGAAC ATGATGTCTT GATCTTCAGA GAGAACACCA TTGCCACCAA ATCCATTGAG 
GAATACCTCA AGCTGGTGGG ACAACAGTAT CTTCACGACG CACTGGGGGA GTTCATCAAA 
GCTTTGTATG AGTCAGATGA AAACTGTGAA GTGGATCCCA GCAAATGTTC ATCCAGTGAA 
CTTCTGGACC ATCAGAGCAA CCTGAAAATG TGCTGTGAGC TGGCTTTCTG CAAGATCATC 
AACTCTTACT GTGTTTTTCC TCGTGAGCTG AAAGAAGTGT TTGCATCTTG GAAGCACCAG 
TGCCTGAACC GCGGCAAGCA GGACATCAGT GAGCGGCTCA TCAGCGCCTC ACTGTTTCTC 
CGTTTTCTGT GCCCGGCCAT CATGTCTCCC AGTCTCTTCA ACCTCATGCA GGAGTATCCT 
GATGACCGCA CATCTCGGAC TCTGACTCTC ATTGCCAAGG TCATCCAGAA CCTGGCCAAC 
TTTGCCAAAT TTGGTAACAA GGAGGAATAC ATGGCATTCA TGAATGATTT TTTGGAACAT 
GAATGGGGTG GAATGAAGCG CTTTCTTTTG GAGATCTCTA ATCCAGACAC TATCTCAAAC 
ACCCCAGGCT TTGATGGTTA CATTGATCTG GGCCGAGAGC TTTCAGTTTT GCATTCCTTA 
CTGTGGGAAG TAGTTTCCCA ACTTGATAAG GCAACCGTGG CAAAATTGGG ACCTCTCCCT 
CGTGTTCTTG CTGATATTAC CAAATCTCTG ACTAATCCTA CACCAATACA ACAGCAACTG 
AGACGCTTCA CTGAGCATAA CTCCAGTCCA AATGTCAGTG GAAGCCTCTC CTCTGGGCTG 
CAGAAAATAT TTGAAGACCC TACTGACAGT GATTTGCACA AACTAAAATC CCCAAGCCAG 
GACAACACAG ACAGTTATTT CAGAGGGAAG ACATTATTGC TGGTCCAGCA AGCCTCCTCC 
CAGAGCATGA CTTACTCGGA AAAGGATGAA AAGGAAAGTA GCCTCCCCAA TGGCCGCAGC 
ATCTCCCTCA TGGACCTTCA GGAGACGCAC GCGGCCCAAG CGGAGCATCC GTCTGTGATA 
CTCGACGTGC CCATGCGCTT GACCGGAAGC CAGCTCTCCA TAACTCAGGT GGCCAGCATC 
AAGCAACTGC GGGAGACCCA GAGCACCCCC CAGAGTGCAC CCCAAGTGCG AAGGCCCCTG 
CACCCAGCCT TGAACCAGCC AGGCAGCCTC CAGCCCTTGT CCTTCCAGAA CCCTGTCTAT 
CACCTCAGTA ACCCCATTCC TGCAATGCCA AAGGCCTCTG TGGACTCCAG TTTGGAGAAC 
CTAAGCACTG CCAGTTCCAG GAGCCAAAGT AACAGTGAAG ACTTCAAACT CAGCGGACCC 
AGCAACAGCA GCATGGAGGA CTTCACCAAA CGGAGCACGC AGAGTGAGGA CTTCTCCAGG 
CGCCACACCA TGCCCGACAG ACACATACCT CTCGCCCTGC CGCGGCAGAA CAGCACGGGG 
CAGACCCAGC TCCGGAAGAT GGACCCGGCG GGGCTGGGCG CCCGAGCCAA AGCCCCGCCA 
TCCCTGCCGC ACAGCGCCTC CCTGCGGAGC ACCGGGAGCA TGTCGGTGGC ATCGGCGGCT 
CTGGTGGCCG AGCCCCTGCA GAACGGGAGC CGGTCCCGAC AGCAGTCCTC TTCCTCCAGA 
GAGAGCCCGG TACCCAAAGT GAGAGCCATC CAGAGGCAGC AGACCCAGCA GGTTCAGTCA 
CCTGTGGACT CCGCCACAAT GTCCCCAGTC GAGAGGACAG CAGCCTGGGT TCTGAACAAT 
GGGCAGTATG AAGAGGACGT GGAAGAGACT GAGCAAAATC AGGACGAAGC CAAGCATGCT 
GAGAAGTACG AACAGGAAAT CACCAAACTG AAGGAGCGCC TGCGAGTGTC CAGCCGGCGA 
CTCGAGGAGT ATGAGCGCCG GTTGCTAGTG CAGGAGCAGC AGATGCAGAA GCTGCTGCTG 
GAGTACAAGG CCCGGCTGGA GGACAGCGAG GAGCGGCTGC GCAGGCAGCA GGAGGAGAAG 
GACAGCCAGA TGAAGAGCAT CATCAGCAGG CTAATGGCTG TGGAAGAGGA ACTGAAGAAG 
GATCATGCTG AGATGCAGGC AGTTATTGAT GCAAAGCAAA AAATAATCGA TGCACAGGTC 
ATAAATGGAG ATGAGATTAT GCAAACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA 
CACCAGACTG ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA 
TGGCATCTCC CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285577.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141362.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu66030
Clone ID Related Accession (Same CDS sequence)	XM_025285577.1
Accession Version	XM_025285577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
CCGGGATTCG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC 
AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC 
CAGTCGCCCT ACACCGACAC GACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC 
CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG 
GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG 
TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC TTCTCTTCGC 
AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT 
TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTATCG 
GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT 
TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATCTTCTGG 
GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT 
ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACC 
ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC 
TGTGCCTTAG TGCACATTCT TCAAAGCACG GGAAGGGCCA AGGATTTTCT GACGGACTTG 
GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGCTGGTGG GACAACAGTA TCTTCACGAC 
GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC 
AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG 
TTTGCATCTT GGAAGCACCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG TGAGCGGCTC 
ATCAGCGCCT CACTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC 
AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG 
GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC 
ATGAATGATT TTTTGGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCAGACA CTATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT 
ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT 
TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG 
GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT 
CAGGAGACGC ACGCGGCCCA AGCGGAGCAT CCGTCTGTGA TACTCGACGT GCCCATGCGC 
TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC 
CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT 
CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG 
GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC 
AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG 
ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CATCCCTGCC GCACAGCGCC 
TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG 
CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTACCCAAA 
GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA 
ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC 
GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA 
ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC 
CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG 
GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC 
ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT 
ATGCAAACAG GAAAAACGGA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141362.1
CDS	124..3996
Translation	MELSLSSGGAAEALSWPEMFPGFESDSPLPPEELDAVVPVSGAMAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTDTTTWERKYCILTDSQLVLLNKEKEIPVDGGQEQQADSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIMQTGKTDRVPGFGQHQTDERADPGEGTVQHAGPKWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_025285577.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285577.1

ORF Insert Sequence:

ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
CCGGGATTCG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC 
AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC 
CAGTCGCCCT ACACCGACAC GACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC 
CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG 
GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG 
TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC TTCTCTTCGC 
AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT 
TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTATCG 
GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT 
TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATCTTCTGG 
GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT 
ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACC 
ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC 
TGTGCCTTAG TGCACATTCT TCAAAGCACG GGAAGGGCCA AGGATTTTCT GACGGACTTG 
GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGCTGGTGG GACAACAGTA TCTTCACGAC 
GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC 
AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG 
TTTGCATCTT GGAAGCACCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG TGAGCGGCTC 
ATCAGCGCCT CACTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC 
AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG 
GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC 
ATGAATGATT TTTTGGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCAGACA CTATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT 
ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT 
TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG 
GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT 
CAGGAGACGC ACGCGGCCCA AGCGGAGCAT CCGTCTGTGA TACTCGACGT GCCCATGCGC 
TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC 
CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT 
CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG 
GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC 
AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG 
ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CATCCCTGCC GCACAGCGCC 
TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG 
CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTACCCAAA 
GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA 
ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC 
GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA 
ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC 
CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG 
GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC 
ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG TCATAAATGG AGATGAGATT 
ATGCAAACAG GAAAAACGGA TCGTGTCCCT GGATTCGGCC AACACCAGAC TGATGAGCGC 
GCTGACCCAG GTGAAGGAAC GGTACAGCAT GCAGGTCCGA AATGGCATCT CCCCCACCAA 
CCCCACCAAG CTTTCCATCA CGGAGAATGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285578.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141363.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu66031
Clone ID Related Accession (Same CDS sequence)	XM_025285578.1
Accession Version	XM_025285578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
CCGGGATTCG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC 
AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC 
CAGTCGCCCT ACACCGACAC GACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC 
CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG 
GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG 
TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC TTCTCTTCGC 
AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT 
TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTATCG 
GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT 
TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATCTTCTGG 
GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT 
ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACC 
ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC 
TGTGCCTTAG TGCACATTCT TCAAAGCACG GGAAGGGCCA AGGATTTTCT GACGGACTTG 
GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGCTGGTGG GACAACAGTA TCTTCACGAC 
GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC 
AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG 
TTTGCATCTT GGAAGCACCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG TGAGCGGCTC 
ATCAGCGCCT CACTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC 
AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG 
GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC 
ATGAATGATT TTTTGGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCAGACA CTATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT 
ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT 
TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG 
GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT 
CAGGAGACGC ACGCGGCCCA AGCGGAGCAT CCGTCTGTGA TACTCGACGT GCCCATGCGC 
TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC 
CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT 
CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG 
GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC 
AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG 
ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CATCCCTGCC GCACAGCGCC 
TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG 
CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTACCCAAA 
GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA 
ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC 
GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA 
ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC 
CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG 
GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC 
ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG GTACAGCATG 
CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141363.1
CDS	124..3987
Translation	MELSLSSGGAAEALSWPEMFPGFESDSPLPPEELDAVVPVSGAMAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTDTTTWERKYCILTDSQLVLLNKEKEIPVDGGQEQQADSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_025285578.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285578.1

ORF Insert Sequence:

ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
CCGGGATTCG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC 
AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC 
CAGTCGCCCT ACACCGACAC GACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC 
CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG 
GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG 
TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC TTCTCTTCGC 
AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT 
TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTATCG 
GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT 
TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATCTTCTGG 
GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT 
ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACC 
ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC 
TGTGCCTTAG TGCACATTCT TCAAAGCACG GGAAGGGCCA AGGATTTTCT GACGGACTTG 
GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGCTGGTGG GACAACAGTA TCTTCACGAC 
GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC 
AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG 
TTTGCATCTT GGAAGCACCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG TGAGCGGCTC 
ATCAGCGCCT CACTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC 
AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG 
GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC 
ATGAATGATT TTTTGGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCAGACA CTATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGGTGAAAAT 
TCCTTCCTAC AGGCAACCGT GGCAAAATTG GGACCTCTCC CTCGTGTTCT TGCTGATATT 
ACCAAATCTC TGACTAATCC TACACCAATA CAACAGCAAC TGAGACGCTT CACTGAGCAT 
AACTCCAGTC CAAATGTCAG TGGAAGCCTC TCCTCTGGGC TGCAGAAAAT ATTTGAAGAC 
CCTACTGACA GTGATTTGCA CAAACTAAAA TCCCCAAGCC AGGACAACAC AGACAGTTAT 
TTCAGAGGGA AGACATTATT GCTGGTCCAG CAAGCCTCCT CCCAGAGCAT GACTTACTCG 
GAAAAGGATG AAAAGGAAAG TAGCCTCCCC AATGGCCGCA GCATCTCCCT CATGGACCTT 
CAGGAGACGC ACGCGGCCCA AGCGGAGCAT CCGTCTGTGA TACTCGACGT GCCCATGCGC 
TTGACCGGAA GCCAGCTCTC CATAACTCAG GTGGCCAGCA TCAAGCAACT GCGGGAGACC 
CAGAGCACCC CCCAGAGTGC ACCCCAAGTG CGAAGGCCCC TGCACCCAGC CTTGAACCAG 
CCAGGCAGCC TCCAGCCCTT GTCCTTCCAG AACCCTGTCT ATCACCTCAG TAACCCCATT 
CCTGCAATGC CAAAGGCCTC TGTGGACTCC AGTTTGGAGA ACCTAAGCAC TGCCAGTTCC 
AGGAGCCAAA GTAACAGTGA AGACTTCAAA CTCAGCGGAC CCAGCAACAG CAGCATGGAG 
GACTTCACCA AACGGAGCAC GCAGAGTGAG GACTTCTCCA GGCGCCACAC CATGCCCGAC 
AGACACATAC CTCTCGCCCT GCCGCGGCAG AACAGCACGG GGCAGACCCA GCTCCGGAAG 
ATGGACCCGG CGGGGCTGGG CGCCCGAGCC AAAGCCCCGC CATCCCTGCC GCACAGCGCC 
TCCCTGCGGA GCACCGGGAG CATGTCGGTG GCATCGGCGG CTCTGGTGGC CGAGCCCCTG 
CAGAACGGGA GCCGGTCCCG ACAGCAGTCC TCTTCCTCCA GAGAGAGCCC GGTACCCAAA 
GTGAGAGCCA TCCAGAGGCA GCAGACCCAG CAGGTTCAGT CACCTGTGGA CTCCGCCACA 
ATGTCCCCAG TCGAGAGGAC AGCAGCCTGG GTTCTGAACA ATGGGCAGTA TGAAGAGGAC 
GTGGAAGAGA CTGAGCAAAA TCAGGACGAA GCCAAGCATG CTGAGAAGTA CGAACAGGAA 
ATCACCAAAC TGAAGGAGCG CCTGCGAGTG TCCAGCCGGC GACTCGAGGA GTATGAGCGC 
CGGTTGCTAG TGCAGGAGCA GCAGATGCAG AAGCTGCTGC TGGAGTACAA GGCCCGGCTG 
GAGGACAGCG AGGAGCGGCT GCGCAGGCAG CAGGAGGAGA AGGACAGCCA GATGAAGAGC 
ATCATCAGCA GGCTAATGGC TGTGGAAGAG GAACTGAAGA AGGATCATGC TGAGATGCAG 
GCAGTTATTG ATGCAAAGCA AAAAATAATC GATGCACAGG AAAAACGGAT CGTGTCCCTG 
GATTCGGCCA ACACCAGACT GATGAGCGCG CTGACCCAGG TGAAGGAACG GTACAGCATG 
CAGGTCCGAA ATGGCATCTC CCCCACCAAC CCCACCAAGC TTTCCATCAC GGAGAATGGT 
GAATTCAAAA ACAGCAGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285580.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141365.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

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CloneID	OBu66032
Clone ID Related Accession (Same CDS sequence)	XM_025285580.1
Accession Version	XM_025285580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
CCGGGATTCG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC 
AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC 
CAGTCGCCCT ACACCGACAC GACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC 
CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG 
GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG 
TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC TTCTCTTCGC 
AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT 
TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTATCG 
GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT 
TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATCTTCTGG 
GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT 
ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACC 
ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC 
TGTGCCTTAG TGCACATTCT TCAAAGCACG GGAAGGGCCA AGGATTTTCT GACGGACTTG 
GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGCTGGTGG GACAACAGTA TCTTCACGAC 
GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC 
AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG 
TTTGCATCTT GGAAGCACCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG TGAGCGGCTC 
ATCAGCGCCT CACTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC 
AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG 
GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC 
ATGAATGATT TTTTGGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCAGACA CTATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG 
GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC 
AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG 
CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT 
AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCGGCCCAA 
GCGGAGCATC CGTCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC 
ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT 
GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA 
GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG 
CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG 
CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC 
GCCCGAGCCA AAGCCCCGCC ATCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC 
ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTACCCAAAG TGAGAGCCAT CCAGAGGCAG 
CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT 
CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141365.1
CDS	124..3966
Translation	MELSLSSGGAAEALSWPEMFPGFESDSPLPPEELDAVVPVSGAMAGGMLDRILLESVCQQQSWVRVYDVKGPPTHRLSCGQSPYTDTTTWERKYCILTDSQLVLLNKEKEIPVDGGQEQQADSTKGRCLRRTVSVPSEGQFPEYPPEGATKLEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQEKRIVSLDSANTRLMSALTQVKERYSMQVRNGISPTNPTKLSITENGEFKNSSC

Target ORF information:

RefSeq Version	XM_025285580.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285580.1

ORF Insert Sequence:

ATGGAGCTCT CCCTGTCGTC CGGAGGAGCC GCGGAGGCGC TGTCCTGGCC AGAGATGTTC 
CCGGGATTCG AGTCCGACTC GCCGCTCCCC CCGGAGGAGC TGGACGCGGT TGTCCCGGTC 
AGTGGGGCCA TGGCCGGTGG CATGTTGGAT CGGATTCTTC TGGAGTCCGT GTGCCAGCAA 
CAGAGCTGGG TCCGCGTGTA CGATGTGAAA GGGCCACCTA CCCACCGTCT GTCTTGTGGC 
CAGTCGCCCT ACACCGACAC GACAACATGG GAGCGGAAGT ACTGCATCCT CACAGACAGC 
CAGCTGGTGT TGCTCAACAA GGAGAAGGAG ATACCTGTGG ACGGAGGACA AGAACAGCAG 
GCGGATTCCA CCAAAGGGCG ATGCCTGCGG AGAACTGTCA GTGTTCCTTC CGAGGGTCAG 
TTTCCTGAAT ACCCACCAGA GGGCGCCACT AAACTGGAGG TGCCAGCAGA AAGGTCCCCA 
CGCAGACGGA GTATCTCAGG GACCACTACA TCTGAGAAAC CCAACTCTAT GGACACTGCG 
AATACCTCAC CCTTCAAAGT ACCAGGGTTC TTCAGCAAGC GCCTAAAAGG CTCCATCAAG 
AGGACCAAAA GCCAATCAAA GCTTGACCGA AACACGAGCT TTCGGCTTCC TTCTCTTCGC 
AATACCGATG ACAGGTCTCG TGGGCTGCCT AAACTAAAAG AGTCCCGTTC TCATGAATCT 
TTGCTGAGCC CATGCAGTGC CGTGGAATGT CTGGATCTCG GTAGAGGGGA GCCTGTATCG 
GTAAAGCCAC TCCATAGCAG CATCCTTGGA CAAGACTTCT GCTTTGAGGT TACCTACTTA 
AGTGGAAGTA AATGCTTCAG TTGTAACTCT GCTTCAGAGA GAGATAAGTG GATGGAAAAC 
CTGCGCAGGA CAGTTCAACC AAATAAGGAC AATTGTAGAC GAGCTGAAAA TGTTCTCCGT 
TTATGGATTA TTGAAGCCAA GGACCTTGCC CCTAAAAAGA AATATTTCTG TGAACTGTGC 
CTTGATGATA CCCTCTTTGC TCGTACAACC AGCAAGACCA AAGCAGACAA TATCTTCTGG 
GGTGAACATT TTGAGTTCTA CAGCCTTCCA CCTCTTCATA GCATCACAGT TCACATTTAT 
AAAGATGTGG AAAAAAAGAA GAAAAAAGAC AAGAATAATT ATGTAGGGCT AGTCAACATC 
CCCACTGCCA GTGTGACTGG TCGCCAGTTT GTAGAAAAGT GGTACCCAGT GAGTACACCT 
ACACCCAACA AAGGAAAGAC AGGAGGACCT TCTATTCGGA TTAAATCACG TTTCCAAACC 
ATCACCATTC TGCCTATGGA GCAATACAAA GAGTTTGCAG AATTTGTCAC CAGCAACTAT 
ACCATGTTAT GTTCTGTTCT TGAACCAGTA ATCAGTGTGA GAAATAAAGA AGAGCTAGCC 
TGTGCCTTAG TGCACATTCT TCAAAGCACG GGAAGGGCCA AGGATTTTCT GACGGACTTG 
GTGATGTCTG AGGTGGATCG TTGTGGAGAA CATGATGTCT TGATCTTCAG AGAGAACACC 
ATTGCCACCA AATCCATTGA GGAATACCTC AAGCTGGTGG GACAACAGTA TCTTCACGAC 
GCACTGGGGG AGTTCATCAA AGCTTTGTAT GAGTCAGATG AAAACTGTGA AGTGGATCCC 
AGCAAATGTT CATCCAGTGA ACTTCTGGAC CATCAGAGCA ACCTGAAAAT GTGCTGTGAG 
CTGGCTTTCT GCAAGATCAT CAACTCTTAC TGTGTTTTTC CTCGTGAGCT GAAAGAAGTG 
TTTGCATCTT GGAAGCACCA GTGCCTGAAC CGCGGCAAGC AGGACATCAG TGAGCGGCTC 
ATCAGCGCCT CACTGTTTCT CCGTTTTCTG TGCCCGGCCA TCATGTCTCC CAGTCTCTTC 
AACCTCATGC AGGAGTATCC TGATGACCGC ACATCTCGGA CTCTGACTCT CATTGCCAAG 
GTCATCCAGA ACCTGGCCAA CTTTGCCAAA TTTGGTAACA AGGAGGAATA CATGGCATTC 
ATGAATGATT TTTTGGAACA TGAATGGGGT GGAATGAAGC GCTTTCTTTT GGAGATCTCT 
AATCCAGACA CTATCTCAAA CACCCCAGGC TTTGATGGTT ACATTGATCT GGGCCGAGAG 
CTTTCAGTTT TGCATTCCTT ACTGTGGGAA GTAGTTTCCC AACTTGATAA GGCAACCGTG 
GCAAAATTGG GACCTCTCCC TCGTGTTCTT GCTGATATTA CCAAATCTCT GACTAATCCT 
ACACCAATAC AACAGCAACT GAGACGCTTC ACTGAGCATA ACTCCAGTCC AAATGTCAGT 
GGAAGCCTCT CCTCTGGGCT GCAGAAAATA TTTGAAGACC CTACTGACAG TGATTTGCAC 
AAACTAAAAT CCCCAAGCCA GGACAACACA GACAGTTATT TCAGAGGGAA GACATTATTG 
CTGGTCCAGC AAGCCTCCTC CCAGAGCATG ACTTACTCGG AAAAGGATGA AAAGGAAAGT 
AGCCTCCCCA ATGGCCGCAG CATCTCCCTC ATGGACCTTC AGGAGACGCA CGCGGCCCAA 
GCGGAGCATC CGTCTGTGAT ACTCGACGTG CCCATGCGCT TGACCGGAAG CCAGCTCTCC 
ATAACTCAGG TGGCCAGCAT CAAGCAACTG CGGGAGACCC AGAGCACCCC CCAGAGTGCA 
CCCCAAGTGC GAAGGCCCCT GCACCCAGCC TTGAACCAGC CAGGCAGCCT CCAGCCCTTG 
TCCTTCCAGA ACCCTGTCTA TCACCTCAGT AACCCCATTC CTGCAATGCC AAAGGCCTCT 
GTGGACTCCA GTTTGGAGAA CCTAAGCACT GCCAGTTCCA GGAGCCAAAG TAACAGTGAA 
GACTTCAAAC TCAGCGGACC CAGCAACAGC AGCATGGAGG ACTTCACCAA ACGGAGCACG 
CAGAGTGAGG ACTTCTCCAG GCGCCACACC ATGCCCGACA GACACATACC TCTCGCCCTG 
CCGCGGCAGA ACAGCACGGG GCAGACCCAG CTCCGGAAGA TGGACCCGGC GGGGCTGGGC 
GCCCGAGCCA AAGCCCCGCC ATCCCTGCCG CACAGCGCCT CCCTGCGGAG CACCGGGAGC 
ATGTCGGTGG CATCGGCGGC TCTGGTGGCC GAGCCCCTGC AGAACGGGAG CCGGTCCCGA 
CAGCAGTCCT CTTCCTCCAG AGAGAGCCCG GTACCCAAAG TGAGAGCCAT CCAGAGGCAG 
CAGACCCAGC AGGTTCAGTC ACCTGTGGAC TCCGCCACAA TGTCCCCAGT CGAGAGGACA 
GCAGCCTGGG TTCTGAACAA TGGGCAGTAT GAAGAGGACG TGGAAGAGAC TGAGCAAAAT 
CAGGACGAAG CCAAGCATGC TGAGAAGTAC GAACAGGAAA TCACCAAACT GAAGGAGCGC 
CTGCGAGTGT CCAGCCGGCG ACTCGAGGAG TATGAGCGCC GGTTGCTAGT GCAGGAGCAG 
CAGATGCAGA AGCTGCTGCT GGAGTACAAG GCCCGGCTGG AGGACAGCGA GGAGCGGCTG 
CGCAGGCAGC AGGAGGAGAA GGACAGCCAG ATGAAGAGCA TCATCAGCAG GCTAATGGCT 
GTGGAAGAGG AACTGAAGAA GGATCATGCT GAGATGCAGG CAGTTATTGA TGCAAAGCAA 
AAAATAATCG ATGCACAGGA AAAACGGATC GTGTCCCTGG ATTCGGCCAA CACCAGACTG 
ATGAGCGCGC TGACCCAGGT GAAGGAACGG TACAGCATGC AGGTCCGAAA TGGCATCTCC 
CCCACCAACC CCACCAAGCT TTCCATCACG GAGAATGGTG AATTCAAAAA CAGCAGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285581.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141366.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu66033
Clone ID Related Accession (Same CDS sequence)	XM_025285581.1
Accession Version	XM_025285581.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCTTCTC TTCGCAATAC CGATGACAGG 
TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC 
AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TATCGGTAAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT 
CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATCT TCTGGGGTGA ACATTTTGAG 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA 
AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT 
GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC 
ATTCTTCAAA GCACGGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG 
GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGCT GGTGGGACAA CAGTATCTTC ACGACGCACT GGGGGAGTTC 
ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC 
AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCTTGGAAG 
CACCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGTGAGC GGCTCATCAG CGCCTCACTG 
TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG 
GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTG 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACTATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA 
ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT 
AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT 
TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA 
TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG 
GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCG 
GCCCAAGCGG AGCATCCGTC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG 
CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCTTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG 
GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC 
AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG 
AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC 
GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG 
CTGGGCGCCC GAGCCAAAGC CCCGCCATCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC 
GGGAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG 
TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTAC CCAAAGTGAG AGCCATCCAG 
AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG 
AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG 
CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG 
GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG 
CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA 
ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCGCTGA CCCAGGTGAA 
GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141366.1
CDS	16..3453
Translation	MGSSCDKEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIMQTGKTDRVPGFGQHQTDERADPGEGTVQHAGPKWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_025285581.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285581.1

ORF Insert Sequence:

ATGGGAAGCA GTTGTGATAA AGAGGTGCCA GCAGAAAGGT CCCCACGCAG ACGGAGTATC 
TCAGGGACCA CTACATCTGA GAAACCCAAC TCTATGGACA CTGCGAATAC CTCACCCTTC 
AAAGTACCAG GGTTCTTCAG CAAGCGCCTA AAAGGCTCCA TCAAGAGGAC CAAAAGCCAA 
TCAAAGCTTG ACCGAAACAC GAGCTTTCGG CTTCCTTCTC TTCGCAATAC CGATGACAGG 
TCTCGTGGGC TGCCTAAACT AAAAGAGTCC CGTTCTCATG AATCTTTGCT GAGCCCATGC 
AGTGCCGTGG AATGTCTGGA TCTCGGTAGA GGGGAGCCTG TATCGGTAAA GCCACTCCAT 
AGCAGCATCC TTGGACAAGA CTTCTGCTTT GAGGTTACCT ACTTAAGTGG AAGTAAATGC 
TTCAGTTGTA ACTCTGCTTC AGAGAGAGAT AAGTGGATGG AAAACCTGCG CAGGACAGTT 
CAACCAAATA AGGACAATTG TAGACGAGCT GAAAATGTTC TCCGTTTATG GATTATTGAA 
GCCAAGGACC TTGCCCCTAA AAAGAAATAT TTCTGTGAAC TGTGCCTTGA TGATACCCTC 
TTTGCTCGTA CAACCAGCAA GACCAAAGCA GACAATATCT TCTGGGGTGA ACATTTTGAG 
TTCTACAGCC TTCCACCTCT TCATAGCATC ACAGTTCACA TTTATAAAGA TGTGGAAAAA 
AAGAAGAAAA AAGACAAGAA TAATTATGTA GGGCTAGTCA ACATCCCCAC TGCCAGTGTG 
ACTGGTCGCC AGTTTGTAGA AAAGTGGTAC CCAGTGAGTA CACCTACACC CAACAAAGGA 
AAGACAGGAG GACCTTCTAT TCGGATTAAA TCACGTTTCC AAACCATCAC CATTCTGCCT 
ATGGAGCAAT ACAAAGAGTT TGCAGAATTT GTCACCAGCA ACTATACCAT GTTATGTTCT 
GTTCTTGAAC CAGTAATCAG TGTGAGAAAT AAAGAAGAGC TAGCCTGTGC CTTAGTGCAC 
ATTCTTCAAA GCACGGGAAG GGCCAAGGAT TTTCTGACGG ACTTGGTGAT GTCTGAGGTG 
GATCGTTGTG GAGAACATGA TGTCTTGATC TTCAGAGAGA ACACCATTGC CACCAAATCC 
ATTGAGGAAT ACCTCAAGCT GGTGGGACAA CAGTATCTTC ACGACGCACT GGGGGAGTTC 
ATCAAAGCTT TGTATGAGTC AGATGAAAAC TGTGAAGTGG ATCCCAGCAA ATGTTCATCC 
AGTGAACTTC TGGACCATCA GAGCAACCTG AAAATGTGCT GTGAGCTGGC TTTCTGCAAG 
ATCATCAACT CTTACTGTGT TTTTCCTCGT GAGCTGAAAG AAGTGTTTGC ATCTTGGAAG 
CACCAGTGCC TGAACCGCGG CAAGCAGGAC ATCAGTGAGC GGCTCATCAG CGCCTCACTG 
TTTCTCCGTT TTCTGTGCCC GGCCATCATG TCTCCCAGTC TCTTCAACCT CATGCAGGAG 
TATCCTGATG ACCGCACATC TCGGACTCTG ACTCTCATTG CCAAGGTCAT CCAGAACCTG 
GCCAACTTTG CCAAATTTGG TAACAAGGAG GAATACATGG CATTCATGAA TGATTTTTTG 
GAACATGAAT GGGGTGGAAT GAAGCGCTTT CTTTTGGAGA TCTCTAATCC AGACACTATC 
TCAAACACCC CAGGCTTTGA TGGTTACATT GATCTGGGCC GAGAGCTTTC AGTTTTGCAT 
TCCTTACTGT GGGAAGTAGT TTCCCAACTT GATAAGGGTG AAAATTCCTT CCTACAGGCA 
ACCGTGGCAA AATTGGGACC TCTCCCTCGT GTTCTTGCTG ATATTACCAA ATCTCTGACT 
AATCCTACAC CAATACAACA GCAACTGAGA CGCTTCACTG AGCATAACTC CAGTCCAAAT 
GTCAGTGGAA GCCTCTCCTC TGGGCTGCAG AAAATATTTG AAGACCCTAC TGACAGTGAT 
TTGCACAAAC TAAAATCCCC AAGCCAGGAC AACACAGACA GTTATTTCAG AGGGAAGACA 
TTATTGCTGG TCCAGCAAGC CTCCTCCCAG AGCATGACTT ACTCGGAAAA GGATGAAAAG 
GAAAGTAGCC TCCCCAATGG CCGCAGCATC TCCCTCATGG ACCTTCAGGA GACGCACGCG 
GCCCAAGCGG AGCATCCGTC TGTGATACTC GACGTGCCCA TGCGCTTGAC CGGAAGCCAG 
CTCTCCATAA CTCAGGTGGC CAGCATCAAG CAACTGCGGG AGACCCAGAG CACCCCCCAG 
AGTGCACCCC AAGTGCGAAG GCCCCTGCAC CCAGCCTTGA ACCAGCCAGG CAGCCTCCAG 
CCCTTGTCCT TCCAGAACCC TGTCTATCAC CTCAGTAACC CCATTCCTGC AATGCCAAAG 
GCCTCTGTGG ACTCCAGTTT GGAGAACCTA AGCACTGCCA GTTCCAGGAG CCAAAGTAAC 
AGTGAAGACT TCAAACTCAG CGGACCCAGC AACAGCAGCA TGGAGGACTT CACCAAACGG 
AGCACGCAGA GTGAGGACTT CTCCAGGCGC CACACCATGC CCGACAGACA CATACCTCTC 
GCCCTGCCGC GGCAGAACAG CACGGGGCAG ACCCAGCTCC GGAAGATGGA CCCGGCGGGG 
CTGGGCGCCC GAGCCAAAGC CCCGCCATCC CTGCCGCACA GCGCCTCCCT GCGGAGCACC 
GGGAGCATGT CGGTGGCATC GGCGGCTCTG GTGGCCGAGC CCCTGCAGAA CGGGAGCCGG 
TCCCGACAGC AGTCCTCTTC CTCCAGAGAG AGCCCGGTAC CCAAAGTGAG AGCCATCCAG 
AGGCAGCAGA CCCAGCAGGT TCAGTCACCT GTGGACTCCG CCACAATGTC CCCAGTCGAG 
AGGACAGCAG CCTGGGTTCT GAACAATGGG CAGTATGAAG AGGACGTGGA AGAGACTGAG 
CAAAATCAGG ACGAAGCCAA GCATGCTGAG AAGTACGAAC AGGAAATCAC CAAACTGAAG 
GAGCGCCTGC GAGTGTCCAG CCGGCGACTC GAGGAGTATG AGCGCCGGTT GCTAGTGCAG 
GAGCAGCAGA TGCAGAAGCT GCTGCTGGAG TACAAGGCCC GGCTGGAGGA CAGCGAGGAG 
CGGCTGCGCA GGCAGCAGGA GGAGAAGGAC AGCCAGATGA AGAGCATCAT CAGCAGGCTA 
ATGGCTGTGG AAGAGGAACT GAAGAAGGAT CATGCTGAGA TGCAGGCAGT TATTGATGCA 
AAGCAAAAAA TAATCGATGC ACAGGTCATA AATGGAGATG AGATTATGCA AACAGGAAAA 
ACGGATCGTG TCCCTGGATT CGGCCAACAC CAGACTGATG AGCGCGCTGA CCCAGGTGAA 
GGAACGGTAC AGCATGCAGG TCCGAAATGG CATCTCCCCC ACCAACCCCA CCAAGCTTTC 
CATCACGGAG AATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL2 ( XM_025285582.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025141367.1 Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBu66034
Clone ID Related Accession (Same CDS sequence)	XM_025285582.1
Accession Version	XM_025285582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ras GTPase-activating protein nGAP isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACCGAAACA CGAGCTTTCG GCTTCCTTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG 
CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG 
GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTATCGGTAA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT 
AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGACAATATC TTCTGGGGTG AACATTTTGA GTTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA 
CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA 
AGCACGGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT 
GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGC TGGTGGGACA ACAGTATCTT CACGACGCAC TGGGGGAGTT CATCAAAGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT 
CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCTTGGAA GCACCAGTGC 
CTGAACCGCG GCAAGCAGGA CATCAGTGAG CGGCTCATCA GCGCCTCACT GTTTCTCCGT 
TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT 
GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT GGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACTAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA 
AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA 
CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG 
GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC 
CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC GGCCCAAGCG 
GAGCATCCGT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA 
ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCC 
TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG 
GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC 
TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG 
AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG 
CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC 
CGAGCCAAAG CCCCGCCATC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG 
TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCGGTA CCCAAAGTGA GAGCCATCCA GAGGCAGCAG 
ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG 
GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA 
CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025141367.1
CDS	168..3596
Translation	MQTPEVPAERSPRRRSISGTTTSEKPNSMDTANTSPFKVPGFFSKRLKGSIKRTKSQSKLDRNTSFRLPSLRNTDDRSRGLPKLKESRSHESLLSPCSAVECLDLGRGEPVSVKPLHSSILGQDFCFEVTYLSGSKCFSCNSASERDKWMENLRRTVQPNKDNCRRAENVLRLWIIEAKDLAPKKKYFCELCLDDTLFARTTSKTKADNIFWGEHFEFYSLPPLHSITVHIYKDVEKKKKKDKNNYVGLVNIPTASVTGRQFVEKWYPVSTPTPNKGKTGGPSIRIKSRFQTITILPMEQYKEFAEFVTSNYTMLCSVLEPVISVRNKEELACALVHILQSTGRAKDFLTDLVMSEVDRCGEHDVLIFRENTIATKSIEEYLKLVGQQYLHDALGEFIKALYESDENCEVDPSKCSSSELLDHQSNLKMCCELAFCKIINSYCVFPRELKEVFASWKHQCLNRGKQDISERLISASLFLRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKGENSFLQATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDEKESSLPNGRSISLMDLQETHAAQAEHPSVILDVPMRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGSLQPLSFQNPVYHLSNPIPAMPKASVDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFTKRSTQSEDFSRRHTMPDRHIPLALPRQNSTGQTQLRKMDPAGLGARAKAPPSLPHSASLRSTGSMSVASAALVAEPLQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQYEEDVEETEQNQDEAKHAEKYEQEITKLKERLRVSSRRLEEYERRLLVQEQQMQKLLLEYKARLEDSEERLRRQQEEKDSQMKSIISRLMAVEEELKKDHAEMQAVIDAKQKIIDAQVINGDEIMQTGKTDRVPGFGQHQTDERADPGEGTVQHAGPKWHLPHQPHQAFHHGEW

Target ORF information:

RefSeq Version	XM_025285582.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis RAS protein activator like 2 (RASAL2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025285582.1

ORF Insert Sequence:

ATGCAGACCC CAGAGGTGCC AGCAGAAAGG TCCCCACGCA GACGGAGTAT CTCAGGGACC 
ACTACATCTG AGAAACCCAA CTCTATGGAC ACTGCGAATA CCTCACCCTT CAAAGTACCA 
GGGTTCTTCA GCAAGCGCCT AAAAGGCTCC ATCAAGAGGA CCAAAAGCCA ATCAAAGCTT 
GACCGAAACA CGAGCTTTCG GCTTCCTTCT CTTCGCAATA CCGATGACAG GTCTCGTGGG 
CTGCCTAAAC TAAAAGAGTC CCGTTCTCAT GAATCTTTGC TGAGCCCATG CAGTGCCGTG 
GAATGTCTGG ATCTCGGTAG AGGGGAGCCT GTATCGGTAA AGCCACTCCA TAGCAGCATC 
CTTGGACAAG ACTTCTGCTT TGAGGTTACC TACTTAAGTG GAAGTAAATG CTTCAGTTGT 
AACTCTGCTT CAGAGAGAGA TAAGTGGATG GAAAACCTGC GCAGGACAGT TCAACCAAAT 
AAGGACAATT GTAGACGAGC TGAAAATGTT CTCCGTTTAT GGATTATTGA AGCCAAGGAC 
CTTGCCCCTA AAAAGAAATA TTTCTGTGAA CTGTGCCTTG ATGATACCCT CTTTGCTCGT 
ACAACCAGCA AGACCAAAGC AGACAATATC TTCTGGGGTG AACATTTTGA GTTCTACAGC 
CTTCCACCTC TTCATAGCAT CACAGTTCAC ATTTATAAAG ATGTGGAAAA AAAGAAGAAA 
AAAGACAAGA ATAATTATGT AGGGCTAGTC AACATCCCCA CTGCCAGTGT GACTGGTCGC 
CAGTTTGTAG AAAAGTGGTA CCCAGTGAGT ACACCTACAC CCAACAAAGG AAAGACAGGA 
GGACCTTCTA TTCGGATTAA ATCACGTTTC CAAACCATCA CCATTCTGCC TATGGAGCAA 
TACAAAGAGT TTGCAGAATT TGTCACCAGC AACTATACCA TGTTATGTTC TGTTCTTGAA 
CCAGTAATCA GTGTGAGAAA TAAAGAAGAG CTAGCCTGTG CCTTAGTGCA CATTCTTCAA 
AGCACGGGAA GGGCCAAGGA TTTTCTGACG GACTTGGTGA TGTCTGAGGT GGATCGTTGT 
GGAGAACATG ATGTCTTGAT CTTCAGAGAG AACACCATTG CCACCAAATC CATTGAGGAA 
TACCTCAAGC TGGTGGGACA ACAGTATCTT CACGACGCAC TGGGGGAGTT CATCAAAGCT 
TTGTATGAGT CAGATGAAAA CTGTGAAGTG GATCCCAGCA AATGTTCATC CAGTGAACTT 
CTGGACCATC AGAGCAACCT GAAAATGTGC TGTGAGCTGG CTTTCTGCAA GATCATCAAC 
TCTTACTGTG TTTTTCCTCG TGAGCTGAAA GAAGTGTTTG CATCTTGGAA GCACCAGTGC 
CTGAACCGCG GCAAGCAGGA CATCAGTGAG CGGCTCATCA GCGCCTCACT GTTTCTCCGT 
TTTCTGTGCC CGGCCATCAT GTCTCCCAGT CTCTTCAACC TCATGCAGGA GTATCCTGAT 
GACCGCACAT CTCGGACTCT GACTCTCATT GCCAAGGTCA TCCAGAACCT GGCCAACTTT 
GCCAAATTTG GTAACAAGGA GGAATACATG GCATTCATGA ATGATTTTTT GGAACATGAA 
TGGGGTGGAA TGAAGCGCTT TCTTTTGGAG ATCTCTAATC CAGACACTAT CTCAAACACC 
CCAGGCTTTG ATGGTTACAT TGATCTGGGC CGAGAGCTTT CAGTTTTGCA TTCCTTACTG 
TGGGAAGTAG TTTCCCAACT TGATAAGGGT GAAAATTCCT TCCTACAGGC AACCGTGGCA 
AAATTGGGAC CTCTCCCTCG TGTTCTTGCT GATATTACCA AATCTCTGAC TAATCCTACA 
CCAATACAAC AGCAACTGAG ACGCTTCACT GAGCATAACT CCAGTCCAAA TGTCAGTGGA 
AGCCTCTCCT CTGGGCTGCA GAAAATATTT GAAGACCCTA CTGACAGTGA TTTGCACAAA 
CTAAAATCCC CAAGCCAGGA CAACACAGAC AGTTATTTCA GAGGGAAGAC ATTATTGCTG 
GTCCAGCAAG CCTCCTCCCA GAGCATGACT TACTCGGAAA AGGATGAAAA GGAAAGTAGC 
CTCCCCAATG GCCGCAGCAT CTCCCTCATG GACCTTCAGG AGACGCACGC GGCCCAAGCG 
GAGCATCCGT CTGTGATACT CGACGTGCCC ATGCGCTTGA CCGGAAGCCA GCTCTCCATA 
ACTCAGGTGG CCAGCATCAA GCAACTGCGG GAGACCCAGA GCACCCCCCA GAGTGCACCC 
CAAGTGCGAA GGCCCCTGCA CCCAGCCTTG AACCAGCCAG GCAGCCTCCA GCCCTTGTCC 
TTCCAGAACC CTGTCTATCA CCTCAGTAAC CCCATTCCTG CAATGCCAAA GGCCTCTGTG 
GACTCCAGTT TGGAGAACCT AAGCACTGCC AGTTCCAGGA GCCAAAGTAA CAGTGAAGAC 
TTCAAACTCA GCGGACCCAG CAACAGCAGC ATGGAGGACT TCACCAAACG GAGCACGCAG 
AGTGAGGACT TCTCCAGGCG CCACACCATG CCCGACAGAC ACATACCTCT CGCCCTGCCG 
CGGCAGAACA GCACGGGGCA GACCCAGCTC CGGAAGATGG ACCCGGCGGG GCTGGGCGCC 
CGAGCCAAAG CCCCGCCATC CCTGCCGCAC AGCGCCTCCC TGCGGAGCAC CGGGAGCATG 
TCGGTGGCAT CGGCGGCTCT GGTGGCCGAG CCCCTGCAGA ACGGGAGCCG GTCCCGACAG 
CAGTCCTCTT CCTCCAGAGA GAGCCCGGTA CCCAAAGTGA GAGCCATCCA GAGGCAGCAG 
ACCCAGCAGG TTCAGTCACC TGTGGACTCC GCCACAATGT CCCCAGTCGA GAGGACAGCA 
GCCTGGGTTC TGAACAATGG GCAGTATGAA GAGGACGTGG AAGAGACTGA GCAAAATCAG 
GACGAAGCCA AGCATGCTGA GAAGTACGAA CAGGAAATCA CCAAACTGAA GGAGCGCCTG 
CGAGTGTCCA GCCGGCGACT CGAGGAGTAT GAGCGCCGGT TGCTAGTGCA GGAGCAGCAG 
ATGCAGAAGC TGCTGCTGGA GTACAAGGCC CGGCTGGAGG ACAGCGAGGA GCGGCTGCGC 
AGGCAGCAGG AGGAGAAGGA CAGCCAGATG AAGAGCATCA TCAGCAGGCT AATGGCTGTG 
GAAGAGGAAC TGAAGAAGGA TCATGCTGAG ATGCAGGCAG TTATTGATGC AAAGCAAAAA 
ATAATCGATG CACAGGTCAT AAATGGAGAT GAGATTATGC AAACAGGAAA AACGGATCGT 
GTCCCTGGAT TCGGCCAACA CCAGACTGAT GAGCGCGCTG ACCCAGGTGA AGGAACGGTA 
CAGCATGCAG GTCCGAAATG GCATCTCCCC CACCAACCCC ACCAAGCTTT CCATCACGGA 
GAATGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RASAL2
Entrez Gene ID	102398436
Full Name	RAS protein activator like 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RASAL2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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