NSUN2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSUN2
Entrez Gene ID	102396321
Full Name	NOP2/Sun RNA methyltransferase family member 2
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006047713.1	XP_006047775.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform X2
XM_006047712.1	XP_006047774.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
XM_006047712.2	XP_006047774.1	tRNA (cytosine(34)-C(5))-methyltransferase
XM_006047714.1	XP_006047776.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform X3

Pathways from BioSystems
Gene Ontology

The following NSUN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSUN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu06273	XM_006047712.2 Latest version!	Bubalus bubalis NOP2/Sun RNA methyltransferase family member 2 (NSUN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBu06274	XM_006047713.1 Latest version!	Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OBu06275	XM_006047714.1 Latest version!	Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OBu06273	XM_006047712.1	Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSUN2 ( XM_006047712.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047774.1 Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu06273
Clone ID Related Accession (Same CDS sequence)	XM_006047712.1 , XM_006047712.2
Accession Version	XM_006047712.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784055.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGCC ATGCAAAAGA GATTCTCCAT TGCTTAAAGA ACAAGTATTT CAAGGAACTG 
GAGGACCTGG AGGTGGATGG GCAGAAAGTT GAAGTTCCAC AGCCCTTGAG TTGGTATCCT 
GAAGAACTTG CCTGGCACAC AAATTTAAGT CGAAAAATCT TGAGAAAGTC TCCACAGCTG 
GAAAAGTTTC ACCAGTTTCT GGTCAGCGAA ACTGAATCTG GAAACATCAG CCGTCAGGAA 
GCCGTCAGCA TGATTCCCCC GCTGTTGCTG AATGCTCACC CTCACCATAA GATCTTAGAT 
ATGTGTGCAG CTCCCGGGTC AAAGACCACA CAGTTGATTG AAATGCTGCA TGCGGACATG 
AACGTTCCCT TCCCAGAGGG CTTTGTCATC GCCAACGACG TGGACAACAA GCGCTGCTAC 
CTGCTGGTGC ACCAGGCCAA GCGGCTGGGC AGCCCCTGCA TCATGGTGGT CAACCACGAC 
GCGTCCTGCA TCCCGCGCCT GCAGGTGGAC GTCAACGGCA GGAAGGAAGT CCTCTTCTAC 
GACCGCATCC TCTGTGACGT CCCTTGCAGT GGAGATGGCA CAATGAGAAA AAACATTGAT 
GTCTGGAAGA AGTGGAGCAC TTTGAACAGC CTGCAGCTGC ATGGTTTACA GCTGCGGATC 
GCAACACGGG GTGCTGAGCA GCTAGTGGAG GGGGGCAGGA TGGTGTACTC TACGTGTTCG 
CTGAATCCCA TCGAGGATGA AGCCGTCATA GCGTCTCTAC TGGAAAAGAG TGAAGGTGCT 
TTGGAGCTCG CCGACGTGTC CTCAGAGTTG CCAGGACTGA AGTGGGTGCC TGGGCTCTCA 
CAGTGGAAGG TGATGACGAA AGATGGGCAG TGGTTCGCAT CCTGGGACGA TGTCCCCCAC 
AACAGACACA CCCAGATCCG GCCGACCATG TTCCCCCCGA AGGACCCCGA GAAGCTTCAG 
GCCATGCACC TGGAGCGGTG TCTTAGAATA TTACCACATC ATCAGAACAC TGGAGGGTTC 
TTTGTGGCGG TGTTGGTGAA GAACTCTTCA ATGCCATGGA ATAAACGTCC CCCAAAGCTG 
CAGGGTGAGC CGGTGGAGCT GCGGGCCCCC GTCCCCCCAA GCCCTGCGGA GCCCATGGCG 
GGCTGCACCT CCAACACCTC AGAGCTGGAG AGCAAGCTGG CACCTGGAAT CAGCGACACA 
GGAGCAGTGG AGAGAGCGGA GAACGTGGAC GACAGTGGGA GTAAGAGGGA CGGCGTGTGC 
GGCCCTCCTC CATCCAAGAA AATGAAGCTG TTTGGGTTTA AGGAAGATCC CTTTGTGTTT 
ATTCCCGAAG ACGACCCGCT GTTTCCACCT ATCCAGAAGT TTTATGCTTT GGACCCCTCG 
TTCCCAAAGG CGAATCTGTT AACCCGTACC ACGGAAGGAA AGAAGAGGCA GCTCTACATG 
GTGTCCAAGG AGCTGAGAAA CGTGCTGCTG AACAACAGCG AGAGGATGAA GGTCATTAAC 
ACCGGGATTA AAGTCTGGTG TCGAAATAAC AGCGGTGAAG AGTTCGACTG TGCTTTCCGG 
TTGGCACAGG AGGGAATATA TACGTTATAT CCGTTTATTA ACTCAAGAAT CATCACCGTA 
TCGATAGAAG ACGTCCAAGT TCTGTTAACC CAGGAAAACC CGTTCTTTAG GAAGCTCAGC 
AGCGAGGCCT ACAGCCAGGT CAAGGACATG GCCAAGGGCA GCGTCGTCCT GAAGTACGAG 
CCGGACCCCA CGAAGCCGGA CACGCTGCAG TGCCCCATCG TGCTGTGCGG CTGGCGCGGC 
AAGGCCTCCG TGCGGACTTT CGTGCCCAGG AACGAGCGGC TGCACTACCT GCGCATGATG 
GGGCTGGAGG TGCCGGTGGG AAAGAAGCGA GAGGGCGCTG CTGCGGTGCC CGGTGCGGCC 
AGCCCCGCGG AGCCGGAAGA CGGGCCAGGT GTGGGCAGCC CCATGGAGCC GGAAGACAGG 
CCGGGTGCGG CCAGCCCCGC GGAGCCGGAA GGTGGGCCGG GTGCCGGCAG CCCCGCGGAG 
CAGGAAGACG GGGCCGGTGC TGAGCAAGAG GCCGGCCCGG ACGCTTCCTC AGGAGGGGAC 
CCAGCGGAGG CTGGCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047774.1
CDS	28..2394
Translation	MGRRSRGRRLQQQQQQRPGSAEDGGKRNGAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWDQFMGALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLGSPCIMVVNHDASCIPRLQVDVNGRKEVLFYDRILCDVPCSGDGTMRKNIDVWKKWSTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWVPGLSQWKVMTKDGQWFASWDDVPHNRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKNSSMPWNKRPPKLQGEPVELRAPVPPSPAEPMAGCTSNTSELESKLAPGISDTGAVERAENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKANLLTRTTEGKKRQLYMVSKELRNVLLNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSIEDVQVLLTQENPFFRKLSSEAYSQVKDMAKGSVVLKYEPDPTKPDTLQCPIVLCGWRGKASVRTFVPRNERLHYLRMMGLEVPVGKKREGAAAVPGAASPAEPEDGPGVGSPMEPEDRPGAASPAEPEGGPGAGSPAEQEDGAGAEQEAGPDASSGGDPAEAGLPR

Target ORF information:

RefSeq Version	XM_006047712.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047712.1

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGCC ATGCAAAAGA GATTCTCCAT TGCTTAAAGA ACAAGTATTT CAAGGAACTG 
GAGGACCTGG AGGTGGATGG GCAGAAAGTT GAAGTTCCAC AGCCCTTGAG TTGGTATCCT 
GAAGAACTTG CCTGGCACAC AAATTTAAGT CGAAAAATCT TGAGAAAGTC TCCACAGCTG 
GAAAAGTTTC ACCAGTTTCT GGTCAGCGAA ACTGAATCTG GAAACATCAG CCGTCAGGAA 
GCCGTCAGCA TGATTCCCCC GCTGTTGCTG AATGCTCACC CTCACCATAA GATCTTAGAT 
ATGTGTGCAG CTCCCGGGTC AAAGACCACA CAGTTGATTG AAATGCTGCA TGCGGACATG 
AACGTTCCCT TCCCAGAGGG CTTTGTCATC GCCAACGACG TGGACAACAA GCGCTGCTAC 
CTGCTGGTGC ACCAGGCCAA GCGGCTGGGC AGCCCCTGCA TCATGGTGGT CAACCACGAC 
GCGTCCTGCA TCCCGCGCCT GCAGGTGGAC GTCAACGGCA GGAAGGAAGT CCTCTTCTAC 
GACCGCATCC TCTGTGACGT CCCTTGCAGT GGAGATGGCA CAATGAGAAA AAACATTGAT 
GTCTGGAAGA AGTGGAGCAC TTTGAACAGC CTGCAGCTGC ATGGTTTACA GCTGCGGATC 
GCAACACGGG GTGCTGAGCA GCTAGTGGAG GGGGGCAGGA TGGTGTACTC TACGTGTTCG 
CTGAATCCCA TCGAGGATGA AGCCGTCATA GCGTCTCTAC TGGAAAAGAG TGAAGGTGCT 
TTGGAGCTCG CCGACGTGTC CTCAGAGTTG CCAGGACTGA AGTGGGTGCC TGGGCTCTCA 
CAGTGGAAGG TGATGACGAA AGATGGGCAG TGGTTCGCAT CCTGGGACGA TGTCCCCCAC 
AACAGACACA CCCAGATCCG GCCGACCATG TTCCCCCCGA AGGACCCCGA GAAGCTTCAG 
GCCATGCACC TGGAGCGGTG TCTTAGAATA TTACCACATC ATCAGAACAC TGGAGGGTTC 
TTTGTGGCGG TGTTGGTGAA GAACTCTTCA ATGCCATGGA ATAAACGTCC CCCAAAGCTG 
CAGGGTGAGC CGGTGGAGCT GCGGGCCCCC GTCCCCCCAA GCCCTGCGGA GCCCATGGCG 
GGCTGCACCT CCAACACCTC AGAGCTGGAG AGCAAGCTGG CACCTGGAAT CAGCGACACA 
GGAGCAGTGG AGAGAGCGGA GAACGTGGAC GACAGTGGGA GTAAGAGGGA CGGCGTGTGC 
GGCCCTCCTC CATCCAAGAA AATGAAGCTG TTTGGGTTTA AGGAAGATCC CTTTGTGTTT 
ATTCCCGAAG ACGACCCGCT GTTTCCACCT ATCCAGAAGT TTTATGCTTT GGACCCCTCG 
TTCCCAAAGG CGAATCTGTT AACCCGTACC ACGGAAGGAA AGAAGAGGCA GCTCTACATG 
GTGTCCAAGG AGCTGAGAAA CGTGCTGCTG AACAACAGCG AGAGGATGAA GGTCATTAAC 
ACCGGGATTA AAGTCTGGTG TCGAAATAAC AGCGGTGAAG AGTTCGACTG TGCTTTCCGG 
TTGGCACAGG AGGGAATATA TACGTTATAT CCGTTTATTA ACTCAAGAAT CATCACCGTA 
TCGATAGAAG ACGTCCAAGT TCTGTTAACC CAGGAAAACC CGTTCTTTAG GAAGCTCAGC 
AGCGAGGCCT ACAGCCAGGT CAAGGACATG GCCAAGGGCA GCGTCGTCCT GAAGTACGAG 
CCGGACCCCA CGAAGCCGGA CACGCTGCAG TGCCCCATCG TGCTGTGCGG CTGGCGCGGC 
AAGGCCTCCG TGCGGACTTT CGTGCCCAGG AACGAGCGGC TGCACTACCT GCGCATGATG 
GGGCTGGAGG TGCCGGTGGG AAAGAAGCGA GAGGGCGCTG CTGCGGTGCC CGGTGCGGCC 
AGCCCCGCGG AGCCGGAAGA CGGGCCAGGT GTGGGCAGCC CCATGGAGCC GGAAGACAGG 
CCGGGTGCGG CCAGCCCCGC GGAGCCGGAA GGTGGGCCGG GTGCCGGCAG CCCCGCGGAG 
CAGGAAGACG GGGCCGGTGC TGAGCAAGAG GCCGGCCCGG ACGCTTCCTC AGGAGGGGAC 
CCAGCGGAGG CTGGCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( XM_006047712.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047774.1 Bubalus bubalis NOP2/Sun RNA methyltransferase family member 2 (NSUN2), mRNA.

Price & Availability↑

CloneID	OBu06273
Clone ID Related Accession (Same CDS sequence)	XM_006047712.1 , XM_006047712.2
Accession Version	XM_006047712.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	tRNA (cytosine(34)-C(5))-methyltransferase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037563.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006047712.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGCC ATGCAAAAGA GATTCTCCAT TGCTTAAAGA ACAAGTATTT CAAGGAACTG 
GAGGACCTGG AGGTGGATGG GCAGAAAGTT GAAGTTCCAC AGCCCTTGAG TTGGTATCCT 
GAAGAACTTG CCTGGCACAC AAATTTAAGT CGAAAAATCT TGAGAAAGTC TCCACAGCTG 
GAAAAGTTTC ACCAGTTTCT GGTCAGCGAA ACTGAATCTG GAAACATCAG CCGTCAGGAA 
GCCGTCAGCA TGATTCCCCC GCTGTTGCTG AATGCTCACC CTCACCATAA GATCTTAGAT 
ATGTGTGCAG CTCCCGGGTC AAAGACCACA CAGTTGATTG AAATGCTGCA TGCGGACATG 
AACGTTCCCT TCCCAGAGGG CTTTGTCATC GCCAACGACG TGGACAACAA GCGCTGCTAC 
CTGCTGGTGC ACCAGGCCAA GCGGCTGGGC AGCCCCTGCA TCATGGTGGT CAACCACGAC 
GCGTCCTGCA TCCCGCGCCT GCAGGTGGAC GTCAACGGCA GGAAGGAAGT CCTCTTCTAC 
GACCGCATCC TCTGTGACGT CCCTTGCAGT GGAGATGGCA CAATGAGAAA AAACATTGAT 
GTCTGGAAGA AGTGGAGCAC TTTGAACAGC CTGCAGCTGC ATGGTTTACA GCTGCGGATC 
GCAACACGGG GTGCTGAGCA GCTAGTGGAG GGGGGCAGGA TGGTGTACTC TACGTGTTCG 
CTGAATCCCA TCGAGGATGA AGCCGTCATA GCGTCTCTAC TGGAAAAGAG TGAAGGTGCT 
TTGGAGCTCG CCGACGTGTC CTCAGAGTTG CCAGGACTGA AGTGGGTGCC TGGGCTCTCA 
CAGTGGAAGG TGATGACGAA AGATGGGCAG TGGTTCGCAT CCTGGGACGA TGTCCCCCAC 
AACAGACACA CCCAGATCCG GCCGACCATG TTCCCCCCGA AGGACCCCGA GAAGCTTCAG 
GCCATGCACC TGGAGCGGTG TCTTAGAATA TTACCACATC ATCAGAACAC TGGAGGGTTC 
TTTGTGGCGG TGTTGGTGAA GAACTCTTCA ATGCCATGGA ATAAACGTCC CCCAAAGCTG 
CAGGGTGAGC CGGTGGAGCT GCGGGCCCCC GTCCCCCCAA GCCCTGCGGA GCCCATGGCG 
GGCTGCACCT CCAACACCTC AGAGCTGGAG AGCAAGCTGG CACCTGGAAT CAGCGACACA 
GGAGCAGTGG AGAGAGCGGA GAACGTGGAC GACAGTGGGA GTAAGAGGGA CGGCGTGTGC 
GGCCCTCCTC CATCCAAGAA AATGAAGCTG TTTGGGTTTA AGGAAGATCC CTTTGTGTTT 
ATTCCCGAAG ACGACCCGCT GTTTCCACCT ATCCAGAAGT TTTATGCTTT GGACCCCTCG 
TTCCCAAAGG CGAATCTGTT AACCCGTACC ACGGAAGGAA AGAAGAGGCA GCTCTACATG 
GTGTCCAAGG AGCTGAGAAA CGTGCTGCTG AACAACAGCG AGAGGATGAA GGTCATTAAC 
ACCGGGATTA AAGTCTGGTG TCGAAATAAC AGCGGTGAAG AGTTCGACTG TGCTTTCCGG 
TTGGCACAGG AGGGAATATA TACGTTATAT CCGTTTATTA ACTCAAGAAT CATCACCGTA 
TCGATAGAAG ACGTCCAAGT TCTGTTAACC CAGGAAAACC CGTTCTTTAG GAAGCTCAGC 
AGCGAGGCCT ACAGCCAGGT CAAGGACATG GCCAAGGGCA GCGTCGTCCT GAAGTACGAG 
CCGGACCCCA CGAAGCCGGA CACGCTGCAG TGCCCCATCG TGCTGTGCGG CTGGCGCGGC 
AAGGCCTCCG TGCGGACTTT CGTGCCCAGG AACGAGCGGC TGCACTACCT GCGCATGATG 
GGGCTGGAGG TGCCGGTGGG AAAGAAGCGA GAGGGCGCTG CTGCGGTGCC CGGTGCGGCC 
AGCCCCGCGG AGCCGGAAGA CGGGCCAGGT GTGGGCAGCC CCATGGAGCC GGAAGACAGG 
CCGGGTGCGG CCAGCCCCGC GGAGCCGGAA GGTGGGCCGG GTGCCGGCAG CCCCGCGGAG 
CAGGAAGACG GGGCCGGTGC TGAGCAAGAG GCCGGCCCGG ACGCTTCCTC AGGAGGGGAC 
CCAGCGGAGG CTGGCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047774.1
CDS	176..2542
Translation	MGRRSRGRRLQQQQQQRPGSAEDGGKRNGAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWDQFMGALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLGSPCIMVVNHDASCIPRLQVDVNGRKEVLFYDRILCDVPCSGDGTMRKNIDVWKKWSTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWVPGLSQWKVMTKDGQWFASWDDVPHNRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKNSSMPWNKRPPKLQGEPVELRAPVPPSPAEPMAGCTSNTSELESKLAPGISDTGAVERAENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKANLLTRTTEGKKRQLYMVSKELRNVLLNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSIEDVQVLLTQENPFFRKLSSEAYSQVKDMAKGSVVLKYEPDPTKPDTLQCPIVLCGWRGKASVRTFVPRNERLHYLRMMGLEVPVGKKREGAAAVPGAASPAEPEDGPGVGSPMEPEDRPGAASPAEPEGGPGAGSPAEQEDGAGAEQEAGPDASSGGDPAEAGLPR

Target ORF information:

RefSeq Version	XM_006047712.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NOP2/Sun RNA methyltransferase family member 2 (NSUN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047712.2

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGCC ATGCAAAAGA GATTCTCCAT TGCTTAAAGA ACAAGTATTT CAAGGAACTG 
GAGGACCTGG AGGTGGATGG GCAGAAAGTT GAAGTTCCAC AGCCCTTGAG TTGGTATCCT 
GAAGAACTTG CCTGGCACAC AAATTTAAGT CGAAAAATCT TGAGAAAGTC TCCACAGCTG 
GAAAAGTTTC ACCAGTTTCT GGTCAGCGAA ACTGAATCTG GAAACATCAG CCGTCAGGAA 
GCCGTCAGCA TGATTCCCCC GCTGTTGCTG AATGCTCACC CTCACCATAA GATCTTAGAT 
ATGTGTGCAG CTCCCGGGTC AAAGACCACA CAGTTGATTG AAATGCTGCA TGCGGACATG 
AACGTTCCCT TCCCAGAGGG CTTTGTCATC GCCAACGACG TGGACAACAA GCGCTGCTAC 
CTGCTGGTGC ACCAGGCCAA GCGGCTGGGC AGCCCCTGCA TCATGGTGGT CAACCACGAC 
GCGTCCTGCA TCCCGCGCCT GCAGGTGGAC GTCAACGGCA GGAAGGAAGT CCTCTTCTAC 
GACCGCATCC TCTGTGACGT CCCTTGCAGT GGAGATGGCA CAATGAGAAA AAACATTGAT 
GTCTGGAAGA AGTGGAGCAC TTTGAACAGC CTGCAGCTGC ATGGTTTACA GCTGCGGATC 
GCAACACGGG GTGCTGAGCA GCTAGTGGAG GGGGGCAGGA TGGTGTACTC TACGTGTTCG 
CTGAATCCCA TCGAGGATGA AGCCGTCATA GCGTCTCTAC TGGAAAAGAG TGAAGGTGCT 
TTGGAGCTCG CCGACGTGTC CTCAGAGTTG CCAGGACTGA AGTGGGTGCC TGGGCTCTCA 
CAGTGGAAGG TGATGACGAA AGATGGGCAG TGGTTCGCAT CCTGGGACGA TGTCCCCCAC 
AACAGACACA CCCAGATCCG GCCGACCATG TTCCCCCCGA AGGACCCCGA GAAGCTTCAG 
GCCATGCACC TGGAGCGGTG TCTTAGAATA TTACCACATC ATCAGAACAC TGGAGGGTTC 
TTTGTGGCGG TGTTGGTGAA GAACTCTTCA ATGCCATGGA ATAAACGTCC CCCAAAGCTG 
CAGGGTGAGC CGGTGGAGCT GCGGGCCCCC GTCCCCCCAA GCCCTGCGGA GCCCATGGCG 
GGCTGCACCT CCAACACCTC AGAGCTGGAG AGCAAGCTGG CACCTGGAAT CAGCGACACA 
GGAGCAGTGG AGAGAGCGGA GAACGTGGAC GACAGTGGGA GTAAGAGGGA CGGCGTGTGC 
GGCCCTCCTC CATCCAAGAA AATGAAGCTG TTTGGGTTTA AGGAAGATCC CTTTGTGTTT 
ATTCCCGAAG ACGACCCGCT GTTTCCACCT ATCCAGAAGT TTTATGCTTT GGACCCCTCG 
TTCCCAAAGG CGAATCTGTT AACCCGTACC ACGGAAGGAA AGAAGAGGCA GCTCTACATG 
GTGTCCAAGG AGCTGAGAAA CGTGCTGCTG AACAACAGCG AGAGGATGAA GGTCATTAAC 
ACCGGGATTA AAGTCTGGTG TCGAAATAAC AGCGGTGAAG AGTTCGACTG TGCTTTCCGG 
TTGGCACAGG AGGGAATATA TACGTTATAT CCGTTTATTA ACTCAAGAAT CATCACCGTA 
TCGATAGAAG ACGTCCAAGT TCTGTTAACC CAGGAAAACC CGTTCTTTAG GAAGCTCAGC 
AGCGAGGCCT ACAGCCAGGT CAAGGACATG GCCAAGGGCA GCGTCGTCCT GAAGTACGAG 
CCGGACCCCA CGAAGCCGGA CACGCTGCAG TGCCCCATCG TGCTGTGCGG CTGGCGCGGC 
AAGGCCTCCG TGCGGACTTT CGTGCCCAGG AACGAGCGGC TGCACTACCT GCGCATGATG 
GGGCTGGAGG TGCCGGTGGG AAAGAAGCGA GAGGGCGCTG CTGCGGTGCC CGGTGCGGCC 
AGCCCCGCGG AGCCGGAAGA CGGGCCAGGT GTGGGCAGCC CCATGGAGCC GGAAGACAGG 
CCGGGTGCGG CCAGCCCCGC GGAGCCGGAA GGTGGGCCGG GTGCCGGCAG CCCCGCGGAG 
CAGGAAGACG GGGCCGGTGC TGAGCAAGAG GCCGGCCCGG ACGCTTCCTC AGGAGGGGAC 
CCAGCGGAGG CTGGCCTGCC CCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( XM_006047713.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047775.1 Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X2, mRNA.

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CloneID	OBu06274
Clone ID Related Accession (Same CDS sequence)	XM_006047713.1
Accession Version	XM_006047713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784055.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGCC ATGCAAAAGA GATTCTCCAT TGCTTAAAGA ACAAGTATTT CAAGGAACTG 
GAGGACCTGG AGGTGGATGG GCAGAAAGTT GAAGTTCCAC AGCCCTTGAG TTGGTATCCT 
GAAGAACTTG CCTGGCACAC AAATTTAAGT CGAAAAATCT TGAGAAAGTC TCCACAGCTG 
GAAAAGTTTC ACCAGTTTCT GGTCAGCGAA ACTGAATCTG GAAACATCAG CCGTCAGGAA 
GCCGTCAGCA TGATTCCCCC GCTGTTGCTG AATGCTCACC CTCACCATAA GATCTTAGAT 
ATGTGTGCAG CTCCCGGGTC AAAGACCACA CAGTTGATTG AAATGCTGCA TGCGGACATG 
AACGTTCCCT TCCCAGAGGG CTTTGTCATC GCCAACGACG TGGACAACAA GCGCTGCTAC 
CTGCTGGTGC ACCAGGCCAA GCGGCTGGGC AGCCCCTGCA TCATGGTGGT CAACCACGAC 
GCGTCCTGCA TCCCGCGCCT GCAGGTGGAC GTCAACGGCA GGAAGGAAGT CCTCTTCTAC 
GACCGCATCC TCTGTGACGT CCCTTGCAGT GGAGATGGCA CAATGAGAAA AAACATTGAT 
GTCTGGAAGA AGTGGAGCAC TTTGAACAGC CTGCAGCTGC ATGGTTTACA GCTGCGGATC 
GCAACACGGG GTGCTGAGCA GCTAGTGGAG GGGGGCAGGA TGGTGTACTC TACGTGTTCG 
CTGAATCCCA TCGAGGATGA AGCCGTCATA GCGTCTCTAC TGGAAAAGAG TGAAGGTGCT 
TTGGAGCTCG CCGACGTGTC CTCAGAGTTG CCAGGACTGA AGTGGGTGCC TGGGCTCTCA 
CAGTGGAAGG TGATGACGAA AGATGGGCAG TGGTTCGCAT CCTGGGACGA TGTCCCCCAC 
AACAGACACA CCCAGATCCG GCCGACCATG TTCCCCCCGA AGGACCCCGA GAAGCTTCAG 
GCCATGCACC TGGAGCGGTG TCTTAGAATA TTACCACATC ATCAGAACAC TGGAGGGTTC 
TTTGTGGCGG TGTTGGTGAA GAACTCTTCA ATGCCATGGA ATAAACGTCC CCCAAAGCTG 
CAGGGTGAGC CGGTGGAGCT GCGGGCCCCC GTCCCCCCAA GCCCTGCGGA GCCCATGGCG 
GGCTGCACCT CCAACACCTC AGAGCTGGAG AGCAAGCTGG CACCTGGAAT CAGCGACACA 
GGAGCAGTGG AGAGAGCGGA GAACGTGGAC GACAGTGGGA GTAAGAGGGA CGGCGTGTGC 
GGCCCTCCTC CATCCAAGAA AATGAAGCTG TTTGGGTTTA AGGAAGATCC CTTTGTGTTT 
ATTCCCGAAG ACGACCCGCT GTTTCCACCT ATCCAGAAGT TTTATGCTTT GGACCCCTCG 
TTCCCAAAGG CGAATCTGTT AACCCGTACC ACGGAAGGAA AGAAGAGGCA GCTCTACATG 
GTGTCCAAGG AGCTGAGAAA CGTGCTGCTG AACAACAGCG AGAGGATGAA GGTCATTAAC 
ACCGGGATTA AAGTCTGGTG TCGAAATAAC AGCGGTGAAG AGTTCGACTG TGCTTTCCGG 
TTGGCACAGG AGGGAATATA TACGTTATAT CCGTTTATTA ACTCAAGAAT CATCACCGTA 
TCGATAGAAG ACGTCCAAGT TCTGTTAACC CAGGAAAACC CGTTCTTTAG GAAGCTCAGC 
AGCGAGGCCT ACAGCCAGGT CAAGGACATG GCCAAGGGCA GCGTCGTCCT GAAGTACGAG 
CCGGACCCCA CGAAGCCGGA CACGCTGCAG TGCCCCATCG TGCTGTGCGG CTGGCGCGGC 
AAGGCCTCCG TGCGGACTTT CGTGCCCAGG AACGAGCGGC TGCACTACCT GCGCATGATG 
GGGCTGGAGG TGCCGGTGGG AAAGAAGCGA GAGGGCGCTG CTGCGGTGCC CGGTGCGGCC 
AGCCCCGCGG AGCCGGAAGA CGGGCCGGGT GCGGCCAGCC CCGCGGAGCC GGAAGGTGGG 
CCGGGTGCCG GCAGCCCCGC GGAGCAGGAA GACGGGGCCG GTGCTGAGCA AGAGGCCGGC 
CCGGACGCTT CCTCAGGAGG GGACCCAGCG GAGGCTGGCC TGCCCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047775.1
CDS	29..2359
Translation	MGRRSRGRRLQQQQQQRPGSAEDGGKRNGAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWDQFMGALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLGSPCIMVVNHDASCIPRLQVDVNGRKEVLFYDRILCDVPCSGDGTMRKNIDVWKKWSTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWVPGLSQWKVMTKDGQWFASWDDVPHNRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKNSSMPWNKRPPKLQGEPVELRAPVPPSPAEPMAGCTSNTSELESKLAPGISDTGAVERAENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKANLLTRTTEGKKRQLYMVSKELRNVLLNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSIEDVQVLLTQENPFFRKLSSEAYSQVKDMAKGSVVLKYEPDPTKPDTLQCPIVLCGWRGKASVRTFVPRNERLHYLRMMGLEVPVGKKREGAAAVPGAASPAEPEDGPGAASPAEPEGGPGAGSPAEQEDGAGAEQEAGPDASSGGDPAEAGLPR

Target ORF information:

RefSeq Version	XM_006047713.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047713.1

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGCC ATGCAAAAGA GATTCTCCAT TGCTTAAAGA ACAAGTATTT CAAGGAACTG 
GAGGACCTGG AGGTGGATGG GCAGAAAGTT GAAGTTCCAC AGCCCTTGAG TTGGTATCCT 
GAAGAACTTG CCTGGCACAC AAATTTAAGT CGAAAAATCT TGAGAAAGTC TCCACAGCTG 
GAAAAGTTTC ACCAGTTTCT GGTCAGCGAA ACTGAATCTG GAAACATCAG CCGTCAGGAA 
GCCGTCAGCA TGATTCCCCC GCTGTTGCTG AATGCTCACC CTCACCATAA GATCTTAGAT 
ATGTGTGCAG CTCCCGGGTC AAAGACCACA CAGTTGATTG AAATGCTGCA TGCGGACATG 
AACGTTCCCT TCCCAGAGGG CTTTGTCATC GCCAACGACG TGGACAACAA GCGCTGCTAC 
CTGCTGGTGC ACCAGGCCAA GCGGCTGGGC AGCCCCTGCA TCATGGTGGT CAACCACGAC 
GCGTCCTGCA TCCCGCGCCT GCAGGTGGAC GTCAACGGCA GGAAGGAAGT CCTCTTCTAC 
GACCGCATCC TCTGTGACGT CCCTTGCAGT GGAGATGGCA CAATGAGAAA AAACATTGAT 
GTCTGGAAGA AGTGGAGCAC TTTGAACAGC CTGCAGCTGC ATGGTTTACA GCTGCGGATC 
GCAACACGGG GTGCTGAGCA GCTAGTGGAG GGGGGCAGGA TGGTGTACTC TACGTGTTCG 
CTGAATCCCA TCGAGGATGA AGCCGTCATA GCGTCTCTAC TGGAAAAGAG TGAAGGTGCT 
TTGGAGCTCG CCGACGTGTC CTCAGAGTTG CCAGGACTGA AGTGGGTGCC TGGGCTCTCA 
CAGTGGAAGG TGATGACGAA AGATGGGCAG TGGTTCGCAT CCTGGGACGA TGTCCCCCAC 
AACAGACACA CCCAGATCCG GCCGACCATG TTCCCCCCGA AGGACCCCGA GAAGCTTCAG 
GCCATGCACC TGGAGCGGTG TCTTAGAATA TTACCACATC ATCAGAACAC TGGAGGGTTC 
TTTGTGGCGG TGTTGGTGAA GAACTCTTCA ATGCCATGGA ATAAACGTCC CCCAAAGCTG 
CAGGGTGAGC CGGTGGAGCT GCGGGCCCCC GTCCCCCCAA GCCCTGCGGA GCCCATGGCG 
GGCTGCACCT CCAACACCTC AGAGCTGGAG AGCAAGCTGG CACCTGGAAT CAGCGACACA 
GGAGCAGTGG AGAGAGCGGA GAACGTGGAC GACAGTGGGA GTAAGAGGGA CGGCGTGTGC 
GGCCCTCCTC CATCCAAGAA AATGAAGCTG TTTGGGTTTA AGGAAGATCC CTTTGTGTTT 
ATTCCCGAAG ACGACCCGCT GTTTCCACCT ATCCAGAAGT TTTATGCTTT GGACCCCTCG 
TTCCCAAAGG CGAATCTGTT AACCCGTACC ACGGAAGGAA AGAAGAGGCA GCTCTACATG 
GTGTCCAAGG AGCTGAGAAA CGTGCTGCTG AACAACAGCG AGAGGATGAA GGTCATTAAC 
ACCGGGATTA AAGTCTGGTG TCGAAATAAC AGCGGTGAAG AGTTCGACTG TGCTTTCCGG 
TTGGCACAGG AGGGAATATA TACGTTATAT CCGTTTATTA ACTCAAGAAT CATCACCGTA 
TCGATAGAAG ACGTCCAAGT TCTGTTAACC CAGGAAAACC CGTTCTTTAG GAAGCTCAGC 
AGCGAGGCCT ACAGCCAGGT CAAGGACATG GCCAAGGGCA GCGTCGTCCT GAAGTACGAG 
CCGGACCCCA CGAAGCCGGA CACGCTGCAG TGCCCCATCG TGCTGTGCGG CTGGCGCGGC 
AAGGCCTCCG TGCGGACTTT CGTGCCCAGG AACGAGCGGC TGCACTACCT GCGCATGATG 
GGGCTGGAGG TGCCGGTGGG AAAGAAGCGA GAGGGCGCTG CTGCGGTGCC CGGTGCGGCC 
AGCCCCGCGG AGCCGGAAGA CGGGCCGGGT GCGGCCAGCC CCGCGGAGCC GGAAGGTGGG 
CCGGGTGCCG GCAGCCCCGC GGAGCAGGAA GACGGGGCCG GTGCTGAGCA AGAGGCCGGC 
CCGGACGCTT CCTCAGGAGG GGACCCAGCG GAGGCTGGCC TGCCCCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( XM_006047714.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006047776.1 Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X3, mRNA.

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CloneID	OBu06275
Clone ID Related Accession (Same CDS sequence)	XM_006047714.1
Accession Version	XM_006047714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784055.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGGT ATCCTGAAGA ACTTGCCTGG CACACAAATT TAAGTCGAAA AATCTTGAGA 
AAGTCTCCAC AGCTGGAAAA GTTTCACCAG TTTCTGGTCA GCGAAACTGA ATCTGGAAAC 
ATCAGCCGTC AGGAAGCCGT CAGCATGATT CCCCCGCTGT TGCTGAATGC TCACCCTCAC 
CATAAGATCT TAGATATGTG TGCAGCTCCC GGGTCAAAGA CCACACAGTT GATTGAAATG 
CTGCATGCGG ACATGAACGT TCCCTTCCCA GAGGGCTTTG TCATCGCCAA CGACGTGGAC 
AACAAGCGCT GCTACCTGCT GGTGCACCAG GCCAAGCGGC TGGGCAGCCC CTGCATCATG 
GTGGTCAACC ACGACGCGTC CTGCATCCCG CGCCTGCAGG TGGACGTCAA CGGCAGGAAG 
GAAGTCCTCT TCTACGACCG CATCCTCTGT GACGTCCCTT GCAGTGGAGA TGGCACAATG 
AGAAAAAACA TTGATGTCTG GAAGAAGTGG AGCACTTTGA ACAGCCTGCA GCTGCATGGT 
TTACAGCTGC GGATCGCAAC ACGGGGTGCT GAGCAGCTAG TGGAGGGGGG CAGGATGGTG 
TACTCTACGT GTTCGCTGAA TCCCATCGAG GATGAAGCCG TCATAGCGTC TCTACTGGAA 
AAGAGTGAAG GTGCTTTGGA GCTCGCCGAC GTGTCCTCAG AGTTGCCAGG ACTGAAGTGG 
GTGCCTGGGC TCTCACAGTG GAAGGTGATG ACGAAAGATG GGCAGTGGTT CGCATCCTGG 
GACGATGTCC CCCACAACAG ACACACCCAG ATCCGGCCGA CCATGTTCCC CCCGAAGGAC 
CCCGAGAAGC TTCAGGCCAT GCACCTGGAG CGGTGTCTTA GAATATTACC ACATCATCAG 
AACACTGGAG GGTTCTTTGT GGCGGTGTTG GTGAAGAACT CTTCAATGCC ATGGAATAAA 
CGTCCCCCAA AGCTGCAGGG TGAGCCGGTG GAGCTGCGGG CCCCCGTCCC CCCAAGCCCT 
GCGGAGCCCA TGGCGGGCTG CACCTCCAAC ACCTCAGAGC TGGAGAGCAA GCTGGCACCT 
GGAATCAGCG ACACAGGAGC AGTGGAGAGA GCGGAGAACG TGGACGACAG TGGGAGTAAG 
AGGGACGGCG TGTGCGGCCC TCCTCCATCC AAGAAAATGA AGCTGTTTGG GTTTAAGGAA 
GATCCCTTTG TGTTTATTCC CGAAGACGAC CCGCTGTTTC CACCTATCCA GAAGTTTTAT 
GCTTTGGACC CCTCGTTCCC AAAGGCGAAT CTGTTAACCC GTACCACGGA AGGAAAGAAG 
AGGCAGCTCT ACATGGTGTC CAAGGAGCTG AGAAACGTGC TGCTGAACAA CAGCGAGAGG 
ATGAAGGTCA TTAACACCGG GATTAAAGTC TGGTGTCGAA ATAACAGCGG TGAAGAGTTC 
GACTGTGCTT TCCGGTTGGC ACAGGAGGGA ATATATACGT TATATCCGTT TATTAACTCA 
AGAATCATCA CCGTATCGAT AGAAGACGTC CAAGTTCTGT TAACCCAGGA AAACCCGTTC 
TTTAGGAAGC TCAGCAGCGA GGCCTACAGC CAGGTCAAGG ACATGGCCAA GGGCAGCGTC 
GTCCTGAAGT ACGAGCCGGA CCCCACGAAG CCGGACACGC TGCAGTGCCC CATCGTGCTG 
TGCGGCTGGC GCGGCAAGGC CTCCGTGCGG ACTTTCGTGC CCAGGAACGA GCGGCTGCAC 
TACCTGCGCA TGATGGGGCT GGAGGTGCCG GTGGGAAAGA AGCGAGAGGG CGCTGCTGCG 
GTGCCCGGTG CGGCCAGCCC CGCGGAGCCG GAAGACGGGC CAGGTGTGGG CAGCCCCATG 
GAGCCGGAAG ACAGGCCGGG TGCGGCCAGC CCCGCGGAGC CGGAAGGTGG GCCGGGTGCC 
GGCAGCCCCG CGGAGCAGGA AGACGGGGCC GGTGCTGAGC AAGAGGCCGG CCCGGACGCT 
TCCTCAGGAG GGGACCCAGC GGAGGCTGGC CTGCCCCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006047776.1
CDS	29..2290
Translation	MGRRSRGRRLQQQQQQRPGSAEDGGKRNGAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWDQFMGALREPLPATLRITGYKRYPEELAWHTNLSRKILRKSPQLEKFHQFLVSETESGNISRQEAVSMIPPLLLNAHPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLGSPCIMVVNHDASCIPRLQVDVNGRKEVLFYDRILCDVPCSGDGTMRKNIDVWKKWSTLNSLQLHGLQLRIATRGAEQLVEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSSELPGLKWVPGLSQWKVMTKDGQWFASWDDVPHNRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKNSSMPWNKRPPKLQGEPVELRAPVPPSPAEPMAGCTSNTSELESKLAPGISDTGAVERAENVDDSGSKRDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIQKFYALDPSFPKANLLTRTTEGKKRQLYMVSKELRNVLLNNSERMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSIEDVQVLLTQENPFFRKLSSEAYSQVKDMAKGSVVLKYEPDPTKPDTLQCPIVLCGWRGKASVRTFVPRNERLHYLRMMGLEVPVGKKREGAAAVPGAASPAEPEDGPGVGSPMEPEDRPGAASPAEPEGGPGAGSPAEQEDGAGAEQEAGPDASSGGDPAEAGLPR

Target ORF information:

RefSeq Version	XM_006047714.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis NOP2/Sun RNA methyltransferase family, member 2 (NSUN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006047714.1

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG CCGGCGGCTC CAGCAGCAGC AGCAGCAGCG GCCCGGGAGC 
GCGGAGGACG GCGGAAAGCG CAATGGGGCG GGCTGGGAAG GCGGCTACCC CGAGATCGTC 
AAGGAGAACA AGCTGTTCGA GCACTACTAC CAGGAGCTCA AGATCGTGCC CGAGGGCGAG 
TGGGACCAGT TCATGGGAGC GCTCAGGGAG CCTCTCCCGG CCACTTTAAG AATTACCGGT 
TACAAGAGGT ATCCTGAAGA ACTTGCCTGG CACACAAATT TAAGTCGAAA AATCTTGAGA 
AAGTCTCCAC AGCTGGAAAA GTTTCACCAG TTTCTGGTCA GCGAAACTGA ATCTGGAAAC 
ATCAGCCGTC AGGAAGCCGT CAGCATGATT CCCCCGCTGT TGCTGAATGC TCACCCTCAC 
CATAAGATCT TAGATATGTG TGCAGCTCCC GGGTCAAAGA CCACACAGTT GATTGAAATG 
CTGCATGCGG ACATGAACGT TCCCTTCCCA GAGGGCTTTG TCATCGCCAA CGACGTGGAC 
AACAAGCGCT GCTACCTGCT GGTGCACCAG GCCAAGCGGC TGGGCAGCCC CTGCATCATG 
GTGGTCAACC ACGACGCGTC CTGCATCCCG CGCCTGCAGG TGGACGTCAA CGGCAGGAAG 
GAAGTCCTCT TCTACGACCG CATCCTCTGT GACGTCCCTT GCAGTGGAGA TGGCACAATG 
AGAAAAAACA TTGATGTCTG GAAGAAGTGG AGCACTTTGA ACAGCCTGCA GCTGCATGGT 
TTACAGCTGC GGATCGCAAC ACGGGGTGCT GAGCAGCTAG TGGAGGGGGG CAGGATGGTG 
TACTCTACGT GTTCGCTGAA TCCCATCGAG GATGAAGCCG TCATAGCGTC TCTACTGGAA 
AAGAGTGAAG GTGCTTTGGA GCTCGCCGAC GTGTCCTCAG AGTTGCCAGG ACTGAAGTGG 
GTGCCTGGGC TCTCACAGTG GAAGGTGATG ACGAAAGATG GGCAGTGGTT CGCATCCTGG 
GACGATGTCC CCCACAACAG ACACACCCAG ATCCGGCCGA CCATGTTCCC CCCGAAGGAC 
CCCGAGAAGC TTCAGGCCAT GCACCTGGAG CGGTGTCTTA GAATATTACC ACATCATCAG 
AACACTGGAG GGTTCTTTGT GGCGGTGTTG GTGAAGAACT CTTCAATGCC ATGGAATAAA 
CGTCCCCCAA AGCTGCAGGG TGAGCCGGTG GAGCTGCGGG CCCCCGTCCC CCCAAGCCCT 
GCGGAGCCCA TGGCGGGCTG CACCTCCAAC ACCTCAGAGC TGGAGAGCAA GCTGGCACCT 
GGAATCAGCG ACACAGGAGC AGTGGAGAGA GCGGAGAACG TGGACGACAG TGGGAGTAAG 
AGGGACGGCG TGTGCGGCCC TCCTCCATCC AAGAAAATGA AGCTGTTTGG GTTTAAGGAA 
GATCCCTTTG TGTTTATTCC CGAAGACGAC CCGCTGTTTC CACCTATCCA GAAGTTTTAT 
GCTTTGGACC CCTCGTTCCC AAAGGCGAAT CTGTTAACCC GTACCACGGA AGGAAAGAAG 
AGGCAGCTCT ACATGGTGTC CAAGGAGCTG AGAAACGTGC TGCTGAACAA CAGCGAGAGG 
ATGAAGGTCA TTAACACCGG GATTAAAGTC TGGTGTCGAA ATAACAGCGG TGAAGAGTTC 
GACTGTGCTT TCCGGTTGGC ACAGGAGGGA ATATATACGT TATATCCGTT TATTAACTCA 
AGAATCATCA CCGTATCGAT AGAAGACGTC CAAGTTCTGT TAACCCAGGA AAACCCGTTC 
TTTAGGAAGC TCAGCAGCGA GGCCTACAGC CAGGTCAAGG ACATGGCCAA GGGCAGCGTC 
GTCCTGAAGT ACGAGCCGGA CCCCACGAAG CCGGACACGC TGCAGTGCCC CATCGTGCTG 
TGCGGCTGGC GCGGCAAGGC CTCCGTGCGG ACTTTCGTGC CCAGGAACGA GCGGCTGCAC 
TACCTGCGCA TGATGGGGCT GGAGGTGCCG GTGGGAAAGA AGCGAGAGGG CGCTGCTGCG 
GTGCCCGGTG CGGCCAGCCC CGCGGAGCCG GAAGACGGGC CAGGTGTGGG CAGCCCCATG 
GAGCCGGAAG ACAGGCCGGG TGCGGCCAGC CCCGCGGAGC CGGAAGGTGG GCCGGGTGCC 
GGCAGCCCCG CGGAGCAGGA AGACGGGGCC GGTGCTGAGC AAGAGGCCGG CCCGGACGCT 
TCCTCAGGAG GGGACCCAGC GGAGGCTGGC CTGCCCCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NSUN2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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