HECTD4 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following HECTD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu10681 XM_006053051.1
Latest version!
Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBu51438 XM_025267534.1
Latest version!
Bubalus bubalis HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBu10681
    Clone ID Related Accession (Same CDS sequence) XM_006053051.1
    Accession Version XM_006053051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase HECTD4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784502.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
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    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAACTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CCTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT TTAGGGTCCC TGCCTGTAGC CGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCC GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCCGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGAAACGAG GAGCTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTTATTGACC AGAATACCCT TCAGGTGTGT CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAAGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCGAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CACCCCATTG AAGCACACCA TCTAAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTCTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CCTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCTG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGCGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA TGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT
    AGGAAGGTTG CTGAGTATGG AGGCAATACA CTGGGATATG GCAGCCGTAG TGTCTTAGGA
    ACTGGTTGGC CGAAAGACTT GGTGAAGTGC ATTTTCCCCC CTNTAAATTT TAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCGCTG CCTCTGACCA TTGAAGAAGA CAGAGAGTTC
    ACTTACCCAT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATAT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTGGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTTA ACGAGAACAA AATGAAGGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCCGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG CAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGTCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGTGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATTGCGTG TGCAAATAGC
    ATCGGACTCT TGTGTACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTTC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGTG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCAACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAC GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCCGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG CGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGGG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGG AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCCG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTGTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAATGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGAGCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAA CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCGGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAACTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAACGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCTC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC CCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGACGAT GACGACGATG ACGTCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCAAACAA GTTCACGATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTATC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTTG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCA GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAATGTGGCC ATCACACGGA CCCTGCACCA GGGCGAGGAG
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAATTC
    CCCTCCGAAG AGTTTCCGAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TGGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CGCCACCTCC CACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG TAACAAGCCC GAGGACATGC TGTGGTTCCA TCGTGCTCTG
    ACGTTGCTCA TCATCCTCCG CCACCTCACC AGGAAGGACC CGCAGGGGCT AGGCGTGACA
    AGTGACGCCA TCGCCGACGC CTGCCAGGCC CTAGTGGGCC CCACCGCCCA CAGCCGCTTG
    CTGGTGATCT CTGGGATCCC CACCCACCTG GACGAGGGCA TCGTCAGGGG TGCCATCCGC
    AAGGCCTGCA ATGCCCATGG CGGGGTCTTC AAGGATGAGA TCTACATCCC CCTGCAGGAC
    GAAGACCCCA AGAAGCCAAA AGATAAGGCC GAGGGGGGTG ATGGGAAAGC CGAGCCGGAA
    AAGACCCTCG GCTTCCCCAG CACGGACAGC TTAGAGGTCA GCACGTCCAG CAGCCTGACC
    CCTGCTATGA GCATCAGCGC ATCCGCGTCC ACCAGCCAGG CTTCCATCTG CAGCTCCCAG
    GGCATCTCGC AGACAGTCAG TGACCTCTCT GCGGATCCGT TGCCTGCAGG CCTGGAGCTG
    CCCATCCCTG CAGGCTTGTT GGAGCCCCAT GTGGTGTCCA GCCAGGAGAG CCTGGACATT
    TCCCTGTGTA GCACCGGCAG CCTGGGCAGC CTGGGGAGCC TGGGCGAGCC ACTGGACAAT
    GCAGAAACAG CCTCAGTGTC AGACATGGGC TCCATGTACA CAGTCACGTC CCTGGACAAC
    CAGCCCCTTG CAGCGCGCCC CATCAAAGGC TTCGCGGTCG TGGAGATAAG GTCCCGGGCC
    AAAATTGAGA AAATTCGAGC AAGTTTATTT AACAACAATG ACTTGATAGG TTTGTCAAGC
    TTGGATGGTG AGGATGAATT GATGGAAATG TCAACGGAAG AGATCCTCAC GGTGTCTGTA
    GTGAATCAGA GTCTATTCGA TACGCAGGGG AGCCCAGGGT TAGAAGATTA TTTCAATGAT
    AAGTCAATTA AAGGTGAGAA GCTGGTGCCT GGGGCCAGAG AGGTGCTGAC GGAGATATTT
    AAGAGCTGTG CCCACTCGGA GCAGATGCTG AGCCTGACGC CAGCAAAGCC CATCAAAGTG
    TCGGACATTT ATCTTAGCAA AGAGCAGATC AACTCCCAGA CACCGGGCAA CCTCCTCCAC
    CTTTTCTTCA CCAATGTCCG GCCCCCCAAA AAGGTGCTAG AGGACCAGCT CACCCAGATC
    CTGAGGAAGT ATGGTGTGCC GAAGCCCAAG TTTGACAAGA GCAAGTACAG CAAGGCGGGG
    AAGGAGCAAC ACCCCGGGAA GGTGGTGAGC ACCAAGCGGC CCATCACCAA GCCGCCCACC
    AAGGACAAGG CAGTGCTCAA CAGCGTCAGC AGGACTGCCT TGAGTGAGAA GAAGCCCACC
    GTGAAACCCA AGTCACCCGA AAAGAGCAAG CCAGATGAAA AGGACCCTGA AAAGTCACCC
    ACCAAGAAAC AAGAAGTCCC AGAGGAGAAG TACCTGACCT TAGAAGGATT TCACAAGTTT
    GTGATCGACC GAGCCAAGCA AGACATCCGC AGCGTCTGGA GAGCGATTTT ATCCTGTGGC
    TACGATCTTC ATTTTGAGAG GTGTGCCTGC ATCGACGTCC GGCATGCGCA CAAGGCCTCC
    AGGAAGTGGA CCCTGGAGAT GGATGTGGCG CTCGTGCAGT ACATCAACCG GCTGTGCCGC
    CACCTGGCCA TCACGCCCGC GCGTCTGCAT CCCCACGAGG TGTATCTGGA CCCTGCAGAC
    GCCGCTGACC CCAGAGTGGC CTGTCTCTTG AACGTGCCCA TCGAGAGCCT GCGCCTGCGC
    TTCGCTCTGC TGCAGTCCCT CAACACCACG CTCGAGACCT TCTTCCTGCC GCTGGTGGAG
    CTGCGCCAGA CTCCCATGTA CACGCACAGC ATCGCTGCCC TGCTCAAGGA GGCCAAAGGG
    CTGATCTTTT ATGACACAAA GGTGACCGTC ATGAATCGGG TGCTAAATGC CACGGTGCAG
    AGGACGGCCG ACCATGCGGC ACCAGAGATC ACTCTGGACC CACTGGAGAT CGTGGGAGGG
    GAGATCAGAG CCTCTGAGAA CTCCTACTTC TGCCAGGCAG CCAGGCAGCT GGCCTCAGTG
    CCCTCCTCTC AGCTTTGCGT CAAACTGGCG AGTGGTGGCG ACCCTACCTA TGCCTTCAAC
    ATCCGCTTCA CAGGGGAGGA GGTCCATGGC ACCAGTGGCT CCTTCCGCCA CTTCCTGTGG
    CAGGTGTGCA GAGAGCTGCA GAGCTCCTCG CTCTCCCTCC TCCTGCTGTG CCCCAGCTCC
    GCCGTCAATA AGAACAAGGG CAAGTACATC CTGACCCCCA GCCCCATCAC CTATGGGGAG
    GAGCAGCTGC TGCACTTCCT GGGCCAGTTG CTAGGGATCG CCATCAGAGC AGATGTCCCC
    CTCCCCCTGG ACCTCCTGCC CTCCTTCTGG AAGACACTGG TGGGCGAGCC CCTGGACCCT
    GACCAAGACC TGCAGGAAGC AGACGTACTC ACCTACAATT ACGTCAAGAA ATTTGAGAGT
    ATCAACGACG AGACGGAGTT GGAGGCCCTG TGTGCCGAGA TCGCCTCCCA GCACCTGGCC
    ACTGAGAGCC CCGAGGGGCC CAACAAGCCC TGCTGCCGGT TCACCTACCT GACCATGACC
    GGCGAGGAGG TGGAGCTGTG CAGCCGCGGC CGGCACATCC CCGTGGCGTG GGAGAACAAG
    GACATCTATG CGGCCGCCAT CCGGAGCTTG CGGCTGCGTG AGCTACAGAA CATGGAGTGT
    GTGACGGCCG TGCGCGCGGG CCTCGGGGCC ATCATCCCCC TGCAGCTGCT TACCACGCTC
    AGCCCACTGG AGATGGAGCT GCGCACCTGC GGCCTCCCAT ACATCAACCT CGAGTTCCTG
    AAGGCCCACA CCATGTACCA GGTGGGGCTG ATGGAGACCG ACCAGCACAT CGAGTTCTTC
    TGGGGGGCAC TGGAGATGTT TGCCCAGGAG GAGCTGTGCA AGTTCATCAA GTTTGCCTGC
    AACCAGGAGC GCATCCCGTT CACCTGCCCC TGCAAAGATG GGGGTCCTGA CACGGCCCAT
    GTGCCCCCGT ACCCCATGAA GATTGCCCCC CCGGATGGCA CAGCAGGTTC CCCAGATTCT
    CGCTACATCC GAGTGGAGAC CTGTATGTTC ATGATCAAGC TTCCCCAGTA CTCCTCGCTG
    GAAATCATGC TGGAGAAACT ACGTTGTGCC ATTCACTACC GCGAAGACCC CCTGAGTGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006053113.1
    CDS167..12709
    Translation

    Target ORF information:

    RefSeq Version XM_006053051.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006053051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAACTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CCTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT TTAGGGTCCC TGCCTGTAGC CGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCC GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCCGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGAAACGAG GAGCTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTTATTGACC AGAATACCCT TCAGGTGTGT CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAAGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCGAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CACCCCATTG AAGCACACCA TCTAAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTCTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CCTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCTG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGCGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA TGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT
    AGGAAGGTTG CTGAGTATGG AGGCAATACA CTGGGATATG GCAGCCGTAG TGTCTTAGGA
    ACTGGTTGGC CGAAAGACTT GGTGAAGTGC ATTTTCCCCC CTNTAAATTT TAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCGCTG CCTCTGACCA TTGAAGAAGA CAGAGAGTTC
    ACTTACCCAT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATAT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTGGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTTA ACGAGAACAA AATGAAGGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCCGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG CAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGTCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGTGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATTGCGTG TGCAAATAGC
    ATCGGACTCT TGTGTACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTTC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGTG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCAACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAC GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCCGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG CGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGGG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGG AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCCG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTGTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAATGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGAGCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAA CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCGGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAACTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAACGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCTC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC CCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGACGAT GACGACGATG ACGTCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCAAACAA GTTCACGATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTATC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTTG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCA GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAATGTGGCC ATCACACGGA CCCTGCACCA GGGCGAGGAG
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAATTC
    CCCTCCGAAG AGTTTCCGAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TGGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CGCCACCTCC CACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG TAACAAGCCC GAGGACATGC TGTGGTTCCA TCGTGCTCTG
    ACGTTGCTCA TCATCCTCCG CCACCTCACC AGGAAGGACC CGCAGGGGCT AGGCGTGACA
    AGTGACGCCA TCGCCGACGC CTGCCAGGCC CTAGTGGGCC CCACCGCCCA CAGCCGCTTG
    CTGGTGATCT CTGGGATCCC CACCCACCTG GACGAGGGCA TCGTCAGGGG TGCCATCCGC
    AAGGCCTGCA ATGCCCATGG CGGGGTCTTC AAGGATGAGA TCTACATCCC CCTGCAGGAC
    GAAGACCCCA AGAAGCCAAA AGATAAGGCC GAGGGGGGTG ATGGGAAAGC CGAGCCGGAA
    AAGACCCTCG GCTTCCCCAG CACGGACAGC TTAGAGGTCA GCACGTCCAG CAGCCTGACC
    CCTGCTATGA GCATCAGCGC ATCCGCGTCC ACCAGCCAGG CTTCCATCTG CAGCTCCCAG
    GGCATCTCGC AGACAGTCAG TGACCTCTCT GCGGATCCGT TGCCTGCAGG CCTGGAGCTG
    CCCATCCCTG CAGGCTTGTT GGAGCCCCAT GTGGTGTCCA GCCAGGAGAG CCTGGACATT
    TCCCTGTGTA GCACCGGCAG CCTGGGCAGC CTGGGGAGCC TGGGCGAGCC ACTGGACAAT
    GCAGAAACAG CCTCAGTGTC AGACATGGGC TCCATGTACA CAGTCACGTC CCTGGACAAC
    CAGCCCCTTG CAGCGCGCCC CATCAAAGGC TTCGCGGTCG TGGAGATAAG GTCCCGGGCC
    AAAATTGAGA AAATTCGAGC AAGTTTATTT AACAACAATG ACTTGATAGG TTTGTCAAGC
    TTGGATGGTG AGGATGAATT GATGGAAATG TCAACGGAAG AGATCCTCAC GGTGTCTGTA
    GTGAATCAGA GTCTATTCGA TACGCAGGGG AGCCCAGGGT TAGAAGATTA TTTCAATGAT
    AAGTCAATTA AAGGTGAGAA GCTGGTGCCT GGGGCCAGAG AGGTGCTGAC GGAGATATTT
    AAGAGCTGTG CCCACTCGGA GCAGATGCTG AGCCTGACGC CAGCAAAGCC CATCAAAGTG
    TCGGACATTT ATCTTAGCAA AGAGCAGATC AACTCCCAGA CACCGGGCAA CCTCCTCCAC
    CTTTTCTTCA CCAATGTCCG GCCCCCCAAA AAGGTGCTAG AGGACCAGCT CACCCAGATC
    CTGAGGAAGT ATGGTGTGCC GAAGCCCAAG TTTGACAAGA GCAAGTACAG CAAGGCGGGG
    AAGGAGCAAC ACCCCGGGAA GGTGGTGAGC ACCAAGCGGC CCATCACCAA GCCGCCCACC
    AAGGACAAGG CAGTGCTCAA CAGCGTCAGC AGGACTGCCT TGAGTGAGAA GAAGCCCACC
    GTGAAACCCA AGTCACCCGA AAAGAGCAAG CCAGATGAAA AGGACCCTGA AAAGTCACCC
    ACCAAGAAAC AAGAAGTCCC AGAGGAGAAG TACCTGACCT TAGAAGGATT TCACAAGTTT
    GTGATCGACC GAGCCAAGCA AGACATCCGC AGCGTCTGGA GAGCGATTTT ATCCTGTGGC
    TACGATCTTC ATTTTGAGAG GTGTGCCTGC ATCGACGTCC GGCATGCGCA CAAGGCCTCC
    AGGAAGTGGA CCCTGGAGAT GGATGTGGCG CTCGTGCAGT ACATCAACCG GCTGTGCCGC
    CACCTGGCCA TCACGCCCGC GCGTCTGCAT CCCCACGAGG TGTATCTGGA CCCTGCAGAC
    GCCGCTGACC CCAGAGTGGC CTGTCTCTTG AACGTGCCCA TCGAGAGCCT GCGCCTGCGC
    TTCGCTCTGC TGCAGTCCCT CAACACCACG CTCGAGACCT TCTTCCTGCC GCTGGTGGAG
    CTGCGCCAGA CTCCCATGTA CACGCACAGC ATCGCTGCCC TGCTCAAGGA GGCCAAAGGG
    CTGATCTTTT ATGACACAAA GGTGACCGTC ATGAATCGGG TGCTAAATGC CACGGTGCAG
    AGGACGGCCG ACCATGCGGC ACCAGAGATC ACTCTGGACC CACTGGAGAT CGTGGGAGGG
    GAGATCAGAG CCTCTGAGAA CTCCTACTTC TGCCAGGCAG CCAGGCAGCT GGCCTCAGTG
    CCCTCCTCTC AGCTTTGCGT CAAACTGGCG AGTGGTGGCG ACCCTACCTA TGCCTTCAAC
    ATCCGCTTCA CAGGGGAGGA GGTCCATGGC ACCAGTGGCT CCTTCCGCCA CTTCCTGTGG
    CAGGTGTGCA GAGAGCTGCA GAGCTCCTCG CTCTCCCTCC TCCTGCTGTG CCCCAGCTCC
    GCCGTCAATA AGAACAAGGG CAAGTACATC CTGACCCCCA GCCCCATCAC CTATGGGGAG
    GAGCAGCTGC TGCACTTCCT GGGCCAGTTG CTAGGGATCG CCATCAGAGC AGATGTCCCC
    CTCCCCCTGG ACCTCCTGCC CTCCTTCTGG AAGACACTGG TGGGCGAGCC CCTGGACCCT
    GACCAAGACC TGCAGGAAGC AGACGTACTC ACCTACAATT ACGTCAAGAA ATTTGAGAGT
    ATCAACGACG AGACGGAGTT GGAGGCCCTG TGTGCCGAGA TCGCCTCCCA GCACCTGGCC
    ACTGAGAGCC CCGAGGGGCC CAACAAGCCC TGCTGCCGGT TCACCTACCT GACCATGACC
    GGCGAGGAGG TGGAGCTGTG CAGCCGCGGC CGGCACATCC CCGTGGCGTG GGAGAACAAG
    GACATCTATG CGGCCGCCAT CCGGAGCTTG CGGCTGCGTG AGCTACAGAA CATGGAGTGT
    GTGACGGCCG TGCGCGCGGG CCTCGGGGCC ATCATCCCCC TGCAGCTGCT TACCACGCTC
    AGCCCACTGG AGATGGAGCT GCGCACCTGC GGCCTCCCAT ACATCAACCT CGAGTTCCTG
    AAGGCCCACA CCATGTACCA GGTGGGGCTG ATGGAGACCG ACCAGCACAT CGAGTTCTTC
    TGGGGGGCAC TGGAGATGTT TGCCCAGGAG GAGCTGTGCA AGTTCATCAA GTTTGCCTGC
    AACCAGGAGC GCATCCCGTT CACCTGCCCC TGCAAAGATG GGGGTCCTGA CACGGCCCAT
    GTGCCCCCGT ACCCCATGAA GATTGCCCCC CCGGATGGCA CAGCAGGTTC CCCAGATTCT
    CGCTACATCC GAGTGGAGAC CTGTATGTTC ATGATCAAGC TTCCCCAGTA CTCCTCGCTG
    GAAATCATGC TGGAGAAACT ACGTTGTGCC ATTCACTACC GCGAAGACCC CCTGAGTGGC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBu51438
    Clone ID Related Accession (Same CDS sequence) XM_025267534.1
    Accession Version XM_025267534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase HECTD4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037561.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAACTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CCTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT TTAGGGTCCC TGCCTGTAGC CGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCC GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCCGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGAAACGAG GAGCTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTTATTGACC AGAATACCCT TCAGGTGTGT CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAAGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCGAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CACCCCATTG AAGCACACCA TCTAAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTCTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CCTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCTG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGCGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA TGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT
    AGGAAGGTTG CTGAGTATGG AGGCAATACA CTGGGATATG GCAGCCGTAG TGTCTTAGGA
    ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA
    ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAGGCTA TGTGGGGCTT TGCTTGCACA
    GTTCGCGCTC AGGAGTCTTC GGAGGATGTG TCAGGAGGCT TACCCTTTCT GGTAGACCTG
    GCTTTAGGTC TATCTGTGTT AGCTTGTTCC ATGTTAAGAA TCCTATACAA CGGACCAGAA
    ATTACCAAAG AAGAAGAAAC CTGTCAGGAG CTGTTGCGGT CCAAACTTTT ACAAAGGTGC
    CAGTGGCAGG TAGAGGCCAA CGGTGTGATC TCCCCTGCCC TTACTCCAAG CCCATCTCCG
    CTGCCTCTGA CCATTGAAGA AGACAGAGAG TTCACTTACC CATCTGATGT CCTCGTGCCT
    CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG
    CTCAGGGAAA TATCTGGGCG TGCTAGACCA CAGTTTAGAC CAAGTATAAA AGAAGTAATT
    CAGCCAGATG TGATGGAGGA AATGGTGGTA TCATGTGTTA TTAAGCACTT GAACTTAGTG
    GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT
    TTACTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG
    CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAGAGTGAAG TTGATGATGC CATGCAGGGG
    AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TTAACGAGAA CAAAATGAAG
    GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC
    ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTTAT TCAGAAACCC
    TCGCACCCAC TGGCTAAAAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG
    TTGCTGCACG TCACCATTGC AGCCCAGTCT GGCCTCACGA GAAGCATCTC AGGGACCCCC
    GCGGAAACAC CGGCTTGTAA ATCAGCCTCC GAAACAAAGG TGATCTCTCA TGCTGTGCGT
    CAGCCTGTTT TCCTTCGCAG CATGTCGGCT CCCTCTGACC TGGAAATGAT CGGCAATGAA
    GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC
    TCCTTCACAC TCCTGCAGTC ATTGGCCATA GAGGACAGCA GGGACAAGCC CACCTACAGT
    GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC
    AGCAGCTTTC TTCTGGCCGC ACAGACGAGA TGGCGGCGGG GAAACACCCG CAAGCAGGCG
    TTGGTGCACA TGCGGGAGCT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACACCTT
    GTGGGTCCAG TGACAATGGT CCTGCAGGGA GGACCCAGAA TTGAAGAACT GACCTGTGGT
    GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT GGTGTCTTTG
    TGCGCACGTT ACCCTATTGC GTGTGCAAAT AGCATCGGAC TCTTGTGTAC TATACCTTAT
    ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG AGTGGCCTTG TTCAGCTCAT GGATCGACTA
    TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG
    GTGGCCACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA
    AAAGAAGAAG GTGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC
    CTTCTCACGA ATCACCTTGC CCGAGCAACC GAGTGCTGTG GCAACCAGGC TGCCGGGAAC
    GACGCCCTCC AGGACGTCCT GAGTCTCCTC AATGATCTCT CGAGGAGTCA TATAGGTAAA
    GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTACT TGACCAACGC
    CCATCCCCAA AGCTGGTTCT TATCATTCTT CAGCTGTGCC GGGCGGCACT CCCGCTGATG
    AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC CCACCCTGGA GCTACTCCGT CCCCTCCCTA
    AACAGCGAGC AGGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA
    AAGCTGGCAG ACTACGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCCGAA
    CCTGACACCA CATTGACAAA AACCAGCCCC AAGAATTCCT TAAAAGGCGA TAAAGATCCC
    GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TCATCCACAA GCGGGAAGAC
    CAGTCGTCCC ATGAAGTTCT GCAGCCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA
    CTTGGTACTG GTGCCAACAT GGAGAAAGTT GTGAAAATGG ACCGAGACAT GACGAAGGGT
    GGCTGCTGTG AGGTGATTAC GGAAGAGGCG GCAGCTGCTC TGCGAAAAGC GACCAAGTGG
    GCACAGTCAG GCCTCATTGT CAGCGTTGGG CCACCCGTGG AGTCCCTCAA CCCAGAGGCC
    GGGAGCGGGC TGTCCACAGG GGACAAGAAG AAGACTGCCC AAACCTCCAT TTGCCGAGAG
    AGGAACTCTG AACTCGCCAG GACTGACCCC GTCCGACCTT TCATCAGTGG GCACGTGGCA
    AACAGCATGG CCGCTGAGGT GATCGCCTTG CTTCATAGCT TGCTGATGGC ACCTGAGTCA
    AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC ACTGATGTAC
    ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA
    AAGCTTGCCA AACTTCAGAG AATTGCCCGC CAGGCTGTGG CTGCACTGTG TGCACTTGGA
    GGCTTCAAAG AGACAATCAA GATCGGGTCT GAGGTCCAGG TTTTAGGTAG AGGAATATCA
    GGAAGCATCG GAGTGGTGGC TTCTATCAAT GAACAGGAAG GTATAGCTAC GGTCAGATTC
    CCACCCATAG ACTGTAGAAA GACTTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT
    AGACTTTGTG TTCCAAGAGC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG
    GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC
    TCACTTCCTG CAACAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT
    GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT
    GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAAGTTC TTAACCTAGT AGCCCAGGAA
    TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC
    TACCGGGACT GTGCTCGGCC CCCGCCGCCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA
    GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCCTCG
    CACCTCACTT CTGTCACCTT CCTAGCAGAC CCCAGTGCGG GGGGCGGTCT GCCCAGGGGC
    ACATTTATCT ACGCCACCTC ACCACTGCCC GTTCAGGCCC CGTCTTTCTA CTGGGAAATT
    GAAATTGTTT CCTATGGAGA TACAGATGAT GATACTGGAC CAATAGTTTC CTTTGGCTTT
    GCTACAGAAG CAGAGAAAAG AGATGGGGCA TGGACTAATC CAGTGGGCAC CTGTCTTTTC
    CATAACAATG GCCGAGCTGT GCATTACAAT GGCTCCAGTT TATTACAGTG GAAGAGCGTT
    CGATTAGACG TAACTCTCTC TCCTGGTGAC GTGGCAGGGA TTGGCTGGGA GAGAACCGAG
    GGGACGCCTC CTCCACCCGG GCAGCCTGCC AAGGGCCGTG TGTACTTCAC ATACTGTGGG
    CAGCGCCTCT CACCGTATCT CGAAGATGTC TCGGGTGGGA TGTGGCCGGT GGTTCATATT
    CAGAAAAAGA ACACCAAGAC TCGCGCTAAC TTTGGCTCCC GCCCATTTGC CTACGCTGAA
    GGGCAAGCCC ACCGCAACGC TGCCGACCTG TGCACTGACC TGGCCGAGGA AATTAGCGCT
    AACTTCGAGG CTCTGCCCTT TGCCATGGCC TCTGACAGTG ATAACGATGC TGGCACCAGT
    ATTGCGTCAG ACCCGGGCAC TCATGGGCCA CCATGCCGAA TTGCTGCCGT AGCCACTGCT
    CAGCAACAAT ATGATAGTGA TACTTCCTGT CATTATAAAG TGGAGCTCAG CTATGAGAAT
    TTCATCACCT CTGGGCCAGA CCCCCACCCA CCCCCCATTG CAGATGACGA AAGTGATGAC
    GATGACGACG ATGACGTCCC ACAGGAGGAT CACTATGCCC TGCTGGTGAA AGCCTGGGAG
    ACCAAGGTTT TTCCTACCAT CCGAAGACGG TTCCGCAACG AAGCAGAGAG GAAATCAGGA
    CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT
    GAATTCCTCT ACGAGGAGAA TGGTGGCATC CCAAGAGACC TTTATCTTCC CACCATTGAA
    GACATTAAAG ATGAAGCAAA CAAGTTCACG ATTGATAAAG TTCGAAAAGG TCTCACGGTA
    GTAACACGCT CTCCAGACAG CAATAACGTA GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA
    AAATTTGCTA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT
    GATTCAGTTA ATGAACTAGT TCAGGTAGAA ACGTACCTCC GAAGTGAAGG TGTGCTGGTC
    CGATACTGGT ATCCTATTGA TATGTTGGAA AGGCCCCCCG CAGGCTACAG AAGGACTGCC
    ACCAATGGGC TTGTCACACT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC
    TGCGAGGCCG CGCTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC
    AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCCG CCATCCGGGA CATTACCCTG
    GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC
    AGCTCCAGCT CCATCCTCCT GGCGCAGTCC CTGCAGCACT GTGTCCATTC CCAGAACTGT
    TCAGCCACAG ACCTCTTCTA CCAGGGCAGC TCCCAGACGG TGCGAGAGTG GCTCAATGTG
    GCCATCACAC GGACCCTGCA CCAGGGCGAG GAGAGCCTTC TGGAGCTGAC CAAACAGATC
    TGCTCTTTCC TGCAGACAGC ACCAGAACAA TTCCCCTCCG AAGAGTTTCC GATTTCCGAG
    TCCAAAGTCA ACATGGACGT CAATTTCCCC GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA
    GAAAGTCAGT CTGGATTCCG CAAAGATTCC TCTCTGTACA AGGCACCATG GGCGCGTGTG
    CTGGTGTACG GGCTGGGCCA CAAAGTGAAG CGAAATGGCC AGCTGAACCT CATTGAGGCC
    GTCTGTTACC CGCGGGATGC GTCTCCGGCC AACACAGGGC TCACGCCACC TCCCACCACC
    AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTATCT
    CCCCCACCCG GAGCCGTGCT GGTGTTACAC TCCCTGCCCC TCGAGTTCCC CCTGGCGATG
    GCCTTTGCCG AGCAGCTGCT GTCCTGGAAA TCAGAGGATG GCGAAGGGAG AGCTGAAGAT
    GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTACAAGTGG TGGAGCTGCT CGGCAACTTC
    CTCTGGACCA CAGACATGGC GGCCTGCGTG AAGGAGCTGG TTTTCCACCT CCTGGCAGAG
    CTCCTGCGCA CGGTGCACAG CCTAGAGCAG AGGAAGCACC CAGCTGGCCT GTCCTCGTCC
    ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC GGAGCTTCAC
    AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GTGCATGCGG AGGCTCGGGG
    GCCACGGCGG CTGCTGACCA GGGCCGGTTC TCGACGTATT TCCATGCACT CATGGAAGGC
    TGCCTGGCGG TGGCCGAGGT GACCCTGCCC ACCAACATGA GTGTTACCGC CAGTGGGGTG
    ACGTCAGCGA CTGCCCCAAA CCTCAGCGAC TCGTCCTCCT CCTCATCATC CTCCCCAGGA
    CAGACTCCAC AGAGCCCCAG CCTCCTGTCC AAGAGAAAGA AAGTCAAGAT GAAGCGGGAA
    AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCACGCTCTG TGGACTCGGA CCCCGCCATG
    CTGAGCATTG GGGGCAGCAA GCCCGAGGAC ATGCTGTGGT TCCATCGTGC TCTGACGTTG
    CTCATCATCC TCCGCCACCT CACCAGGAAG GACCCGCAGG GGCTAGGCGT GACAAGTGAC
    GCCATCGCCG ACGCCTGCCA GGCCCTAGTG GGCCCCACCG CCCACAGCCG CTTGCTGGTG
    ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GGGGTGCCAT CCGCAAGGCC
    TGCAATGCCC ATGGCGGGGT CTTCAAGGAT GAGATCTACA TCCCCCTGCA GGACGAAGAC
    CCCAAGAAGC CAAAAGATAA GGCCGAGGGG GGTGATGGGA AAGCCGAGCC GGAAAAGACC
    CTCGGCTTCC CCAGCACGGA CAGCTTAGAG GTCAGCACGT CCAGCAGCCT GACCCCTGCT
    ATGAGCATCA GCGCATCCGC GTCCACCAGC CAGGCTTCCA TCTGCAGCTC CCAGGGCATC
    TCGCAGACAG TCAGTGACCT CTCCGCGGAT CCGTTGCCTG CAGGCCTGGA GCTGCCCATC
    CCTGCAGGCT TGTTGGAGCC CCATGTGGTG TCCAGCCAGG AGAGCCTGGA CATTTCCCTG
    TGTAGCACCG GCAGCCTGGG CAGCCTGGGG AGCCTGGGCG AGCCACTGGA CAATGCAGAA
    ACAGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC
    CTTGCAGCGC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TAAGGTCCCG GGCCAAAATT
    GAGAAAATTC GAGCAAGTTT ATTTAACAAC AATGACTTGA TAGGTTTGTC AAGCTTGGAT
    GGTGAGGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACGGTGTC TGTAGTGAAT
    CAGAGTCTAT TCGATACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA
    ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC
    TGTGCCCACT CGGAGCAGAT GCTGAGCCTG ACGCCAGCAA AGCCCATCAA AGTGTCGGAC
    ATTTATCTTA GCAAAGAGCA GATCAACTCC CAGACACCGG GCAACCTCCT CCACCTTTTC
    TTCACCAATG TCCGGCCCCC CAAAAAGGTG CTAGAGGACC AGCTCACCCA GATCCTGAGG
    AAGTATGGTG TGCCGAAGCC CAAGTTTGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG
    CAACACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCGCC CACCAAGGAC
    AAGGCAGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGTG AGAAGAAGCC CACCGTGAAA
    CCCAAGTCAC CCGAAAAGAG CAAGCCAGAT GAAAAGGACC CTGAAAAGTC ACCCACCAAG
    AAACAAGAAG TCCCAGAGGA GAAGTACCTG ACCTTAGAAG GATTTCACAA GTTTGTGATC
    GACCGAGCCA AGCAAGACAT CCGCAGCGTC TGGAGAGCGA TTTTATCCTG TGGCTACGAT
    CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCATG CGCACAAGGC CTCCAGGAAG
    TGGACCCTGG AGATGGATGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG
    GCCATCACGC CCGCGCGTCT GCATCCCCAC GAGGTGTATC TGGACCCTGC AGACGCCGCT
    GACCCCAGAG TGGCCTGTCT CTTGAACGTG CCCATCGAGA GCCTGCGCCT GCGCTTCGCT
    CTGCTGCAGT CCCTCAACAC CACGCTTGAG ACCTTCTTCC TGCCGCTGGT GGAGCTGCGC
    CAGACTCCCA TGTACACGCA CAGCATCGCT GCCCTGCTCA AGGAGGCCAA AGGGCTGATC
    TTTTATGACA CAAAGGTGAC TGTCATGAAT CGGGTGCTAA ATGCCACGGT GCAGAGGACG
    GCCGACCATG CGGCACCAGA GATCACTCTG GACCCACTGG AGATCGTGGG AGGGGAGATC
    AGAGCCTCTG AGAACTCCTA CTTCTGCCAG GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC
    TCTCAGCTTT GCGTCAAACT GGCGAGTGGT GGCGACCCTA CCTATGCCTT CAACATCCGC
    TTCNACGGGG AGGAGGTCCA TGGCACCAGT GGCTCCTTCC GCCACTTCCT GTGGCAGGTG
    TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC
    AACAAGAACA AGGGCAAGTA CATCCTGACC CCCAGCCCCA TCACCTATGG GGAGGAGCAG
    CTGCTGCACT TCCTGGGCCA GTTGCTAGGG ATCGCCATCA GAGCAGATGT CCCCCTCCCC
    CTGGACCTCC TGCCCTCCTT CTGGAAGACA CTGGTGGGCG AGCCCCTGGA CCCTGACCAA
    GACCTGCAGG AAGCAGACGT ACTCACCTAC AATTACGTCA AGAAATTTGA GAGTATCAAC
    GACGAGACGG AGTTGGAGGC CCTGTGTGCC GAGATCGCCT CCCAGCACCT GGCCACTGAG
    AGCCCCGAGG GGCCCAACAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GACCGGCGAG
    GAGGTGGAGC TGTGCAGCCG CGGCCGGCAC ATCCCCGTGG CGTGGGAGAA CAAGGACATC
    TATGCGGCCG CCATCCGGAG CTTGCGGCTG CGTGAGCTAC AGAACATGGA GTGTGTGACG
    GCCGTGCGCG CGGGCCTCGG GGCCATCATC CCCCTGCAGC TGCTTACCAC GCTCAGCCCA
    CTGGAGATGG AGCTGCGCAC CTGCGGCCTC CCATACATCA ACCTCGAGTT CCTGAAGGCC
    CACACCATGT ACCAGGTGGG GCTGATGGAG ACCGACCAGC ACATCGAGTT CTTCTGGGGG
    GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAG
    GAGCGCATCC CGTTCACCTG CCCCTGCAAA GATGGGGGTC CTGACACGGC CCATGTGCCC
    CCGTACCCCA TGAAGATTGC CCCCCCGGAT GGCACAGCAG GTTCCCCAGA TTCTCGCTAC
    ATCCGAGTGG AGACCTGTAT GTTCATGATC AAGCTTCCCC AGTACTCCTC GCTGGAAATC
    ATGCTGGAGA AACTACGTTG TGCCATTCAC TACCGCGAAG ACCCCCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025123319.1
    CDS213..13469
    Translation

    Target ORF information:

    RefSeq Version XM_025267534.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis HECT domain E3 ubiquitin protein ligase 4 (HECTD4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025267534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAACTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CCTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CGACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT TTAGGGTCCC TGCCTGTAGC CGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCC GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCCGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGAAACGAG GAGCTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTTATTGACC AGAATACCCT TCAGGTGTGT CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAAGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCGAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAGCAG CACCCCATTG AAGCACACCA TCTAAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTCTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CCTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCTG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGCGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA TGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT
    AGGAAGGTTG CTGAGTATGG AGGCAATACA CTGGGATATG GCAGCCGTAG TGTCTTAGGA
    ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA
    ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAGGCTA TGTGGGGCTT TGCTTGCACA
    GTTCGCGCTC AGGAGTCTTC GGAGGATGTG TCAGGAGGCT TACCCTTTCT GGTAGACCTG
    GCTTTAGGTC TATCTGTGTT AGCTTGTTCC ATGTTAAGAA TCCTATACAA CGGACCAGAA
    ATTACCAAAG AAGAAGAAAC CTGTCAGGAG CTGTTGCGGT CCAAACTTTT ACAAAGGTGC
    CAGTGGCAGG TAGAGGCCAA CGGTGTGATC TCCCCTGCCC TTACTCCAAG CCCATCTCCG
    CTGCCTCTGA CCATTGAAGA AGACAGAGAG TTCACTTACC CATCTGATGT CCTCGTGCCT
    CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG
    CTCAGGGAAA TATCTGGGCG TGCTAGACCA CAGTTTAGAC CAAGTATAAA AGAAGTAATT
    CAGCCAGATG TGATGGAGGA AATGGTGGTA TCATGTGTTA TTAAGCACTT GAACTTAGTG
    GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT
    TTACTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG
    CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAGAGTGAAG TTGATGATGC CATGCAGGGG
    AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TTAACGAGAA CAAAATGAAG
    GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC
    ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTTAT TCAGAAACCC
    TCGCACCCAC TGGCTAAAAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG
    TTGCTGCACG TCACCATTGC AGCCCAGTCT GGCCTCACGA GAAGCATCTC AGGGACCCCC
    GCGGAAACAC CGGCTTGTAA ATCAGCCTCC GAAACAAAGG TGATCTCTCA TGCTGTGCGT
    CAGCCTGTTT TCCTTCGCAG CATGTCGGCT CCCTCTGACC TGGAAATGAT CGGCAATGAA
    GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC
    TCCTTCACAC TCCTGCAGTC ATTGGCCATA GAGGACAGCA GGGACAAGCC CACCTACAGT
    GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC
    AGCAGCTTTC TTCTGGCCGC ACAGACGAGA TGGCGGCGGG GAAACACCCG CAAGCAGGCG
    TTGGTGCACA TGCGGGAGCT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACACCTT
    GTGGGTCCAG TGACAATGGT CCTGCAGGGA GGACCCAGAA TTGAAGAACT GACCTGTGGT
    GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT GGTGTCTTTG
    TGCGCACGTT ACCCTATTGC GTGTGCAAAT AGCATCGGAC TCTTGTGTAC TATACCTTAT
    ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG AGTGGCCTTG TTCAGCTCAT GGATCGACTA
    TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG
    GTGGCCACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA
    AAAGAAGAAG GTGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC
    CTTCTCACGA ATCACCTTGC CCGAGCAACC GAGTGCTGTG GCAACCAGGC TGCCGGGAAC
    GACGCCCTCC AGGACGTCCT GAGTCTCCTC AATGATCTCT CGAGGAGTCA TATAGGTAAA
    GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTACT TGACCAACGC
    CCATCCCCAA AGCTGGTTCT TATCATTCTT CAGCTGTGCC GGGCGGCACT CCCGCTGATG
    AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC CCACCCTGGA GCTACTCCGT CCCCTCCCTA
    AACAGCGAGC AGGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA
    AAGCTGGCAG ACTACGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCCGAA
    CCTGACACCA CATTGACAAA AACCAGCCCC AAGAATTCCT TAAAAGGCGA TAAAGATCCC
    GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TCATCCACAA GCGGGAAGAC
    CAGTCGTCCC ATGAAGTTCT GCAGCCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA
    CTTGGTACTG GTGCCAACAT GGAGAAAGTT GTGAAAATGG ACCGAGACAT GACGAAGGGT
    GGCTGCTGTG AGGTGATTAC GGAAGAGGCG GCAGCTGCTC TGCGAAAAGC GACCAAGTGG
    GCACAGTCAG GCCTCATTGT CAGCGTTGGG CCACCCGTGG AGTCCCTCAA CCCAGAGGCC
    GGGAGCGGGC TGTCCACAGG GGACAAGAAG AAGACTGCCC AAACCTCCAT TTGCCGAGAG
    AGGAACTCTG AACTCGCCAG GACTGACCCC GTCCGACCTT TCATCAGTGG GCACGTGGCA
    AACAGCATGG CCGCTGAGGT GATCGCCTTG CTTCATAGCT TGCTGATGGC ACCTGAGTCA
    AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC ACTGATGTAC
    ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA
    AAGCTTGCCA AACTTCAGAG AATTGCCCGC CAGGCTGTGG CTGCACTGTG TGCACTTGGA
    GGCTTCAAAG AGACAATCAA GATCGGGTCT GAGGTCCAGG TTTTAGGTAG AGGAATATCA
    GGAAGCATCG GAGTGGTGGC TTCTATCAAT GAACAGGAAG GTATAGCTAC GGTCAGATTC
    CCACCCATAG ACTGTAGAAA GACTTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT
    AGACTTTGTG TTCCAAGAGC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG
    GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC
    TCACTTCCTG CAACAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT
    GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT
    GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAAGTTC TTAACCTAGT AGCCCAGGAA
    TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC
    TACCGGGACT GTGCTCGGCC CCCGCCGCCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA
    GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCCTCG
    CACCTCACTT CTGTCACCTT CCTAGCAGAC CCCAGTGCGG GGGGCGGTCT GCCCAGGGGC
    ACATTTATCT ACGCCACCTC ACCACTGCCC GTTCAGGCCC CGTCTTTCTA CTGGGAAATT
    GAAATTGTTT CCTATGGAGA TACAGATGAT GATACTGGAC CAATAGTTTC CTTTGGCTTT
    GCTACAGAAG CAGAGAAAAG AGATGGGGCA TGGACTAATC CAGTGGGCAC CTGTCTTTTC
    CATAACAATG GCCGAGCTGT GCATTACAAT GGCTCCAGTT TATTACAGTG GAAGAGCGTT
    CGATTAGACG TAACTCTCTC TCCTGGTGAC GTGGCAGGGA TTGGCTGGGA GAGAACCGAG
    GGGACGCCTC CTCCACCCGG GCAGCCTGCC AAGGGCCGTG TGTACTTCAC ATACTGTGGG
    CAGCGCCTCT CACCGTATCT CGAAGATGTC TCGGGTGGGA TGTGGCCGGT GGTTCATATT
    CAGAAAAAGA ACACCAAGAC TCGCGCTAAC TTTGGCTCCC GCCCATTTGC CTACGCTGAA
    GGGCAAGCCC ACCGCAACGC TGCCGACCTG TGCACTGACC TGGCCGAGGA AATTAGCGCT
    AACTTCGAGG CTCTGCCCTT TGCCATGGCC TCTGACAGTG ATAACGATGC TGGCACCAGT
    ATTGCGTCAG ACCCGGGCAC TCATGGGCCA CCATGCCGAA TTGCTGCCGT AGCCACTGCT
    CAGCAACAAT ATGATAGTGA TACTTCCTGT CATTATAAAG TGGAGCTCAG CTATGAGAAT
    TTCATCACCT CTGGGCCAGA CCCCCACCCA CCCCCCATTG CAGATGACGA AAGTGATGAC
    GATGACGACG ATGACGTCCC ACAGGAGGAT CACTATGCCC TGCTGGTGAA AGCCTGGGAG
    ACCAAGGTTT TTCCTACCAT CCGAAGACGG TTCCGCAACG AAGCAGAGAG GAAATCAGGA
    CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT
    GAATTCCTCT ACGAGGAGAA TGGTGGCATC CCAAGAGACC TTTATCTTCC CACCATTGAA
    GACATTAAAG ATGAAGCAAA CAAGTTCACG ATTGATAAAG TTCGAAAAGG TCTCACGGTA
    GTAACACGCT CTCCAGACAG CAATAACGTA GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA
    AAATTTGCTA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT
    GATTCAGTTA ATGAACTAGT TCAGGTAGAA ACGTACCTCC GAAGTGAAGG TGTGCTGGTC
    CGATACTGGT ATCCTATTGA TATGTTGGAA AGGCCCCCCG CAGGCTACAG AAGGACTGCC
    ACCAATGGGC TTGTCACACT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC
    TGCGAGGCCG CGCTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC
    AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCCG CCATCCGGGA CATTACCCTG
    GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC
    AGCTCCAGCT CCATCCTCCT GGCGCAGTCC CTGCAGCACT GTGTCCATTC CCAGAACTGT
    TCAGCCACAG ACCTCTTCTA CCAGGGCAGC TCCCAGACGG TGCGAGAGTG GCTCAATGTG
    GCCATCACAC GGACCCTGCA CCAGGGCGAG GAGAGCCTTC TGGAGCTGAC CAAACAGATC
    TGCTCTTTCC TGCAGACAGC ACCAGAACAA TTCCCCTCCG AAGAGTTTCC GATTTCCGAG
    TCCAAAGTCA ACATGGACGT CAATTTCCCC GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA
    GAAAGTCAGT CTGGATTCCG CAAAGATTCC TCTCTGTACA AGGCACCATG GGCGCGTGTG
    CTGGTGTACG GGCTGGGCCA CAAAGTGAAG CGAAATGGCC AGCTGAACCT CATTGAGGCC
    GTCTGTTACC CGCGGGATGC GTCTCCGGCC AACACAGGGC TCACGCCACC TCCCACCACC
    AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTATCT
    CCCCCACCCG GAGCCGTGCT GGTGTTACAC TCCCTGCCCC TCGAGTTCCC CCTGGCGATG
    GCCTTTGCCG AGCAGCTGCT GTCCTGGAAA TCAGAGGATG GCGAAGGGAG AGCTGAAGAT
    GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTACAAGTGG TGGAGCTGCT CGGCAACTTC
    CTCTGGACCA CAGACATGGC GGCCTGCGTG AAGGAGCTGG TTTTCCACCT CCTGGCAGAG
    CTCCTGCGCA CGGTGCACAG CCTAGAGCAG AGGAAGCACC CAGCTGGCCT GTCCTCGTCC
    ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC GGAGCTTCAC
    AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GTGCATGCGG AGGCTCGGGG
    GCCACGGCGG CTGCTGACCA GGGCCGGTTC TCGACGTATT TCCATGCACT CATGGAAGGC
    TGCCTGGCGG TGGCCGAGGT GACCCTGCCC ACCAACATGA GTGTTACCGC CAGTGGGGTG
    ACGTCAGCGA CTGCCCCAAA CCTCAGCGAC TCGTCCTCCT CCTCATCATC CTCCCCAGGA
    CAGACTCCAC AGAGCCCCAG CCTCCTGTCC AAGAGAAAGA AAGTCAAGAT GAAGCGGGAA
    AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCACGCTCTG TGGACTCGGA CCCCGCCATG
    CTGAGCATTG GGGGCAGCAA GCCCGAGGAC ATGCTGTGGT TCCATCGTGC TCTGACGTTG
    CTCATCATCC TCCGCCACCT CACCAGGAAG GACCCGCAGG GGCTAGGCGT GACAAGTGAC
    GCCATCGCCG ACGCCTGCCA GGCCCTAGTG GGCCCCACCG CCCACAGCCG CTTGCTGGTG
    ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GGGGTGCCAT CCGCAAGGCC
    TGCAATGCCC ATGGCGGGGT CTTCAAGGAT GAGATCTACA TCCCCCTGCA GGACGAAGAC
    CCCAAGAAGC CAAAAGATAA GGCCGAGGGG GGTGATGGGA AAGCCGAGCC GGAAAAGACC
    CTCGGCTTCC CCAGCACGGA CAGCTTAGAG GTCAGCACGT CCAGCAGCCT GACCCCTGCT
    ATGAGCATCA GCGCATCCGC GTCCACCAGC CAGGCTTCCA TCTGCAGCTC CCAGGGCATC
    TCGCAGACAG TCAGTGACCT CTCCGCGGAT CCGTTGCCTG CAGGCCTGGA GCTGCCCATC
    CCTGCAGGCT TGTTGGAGCC CCATGTGGTG TCCAGCCAGG AGAGCCTGGA CATTTCCCTG
    TGTAGCACCG GCAGCCTGGG CAGCCTGGGG AGCCTGGGCG AGCCACTGGA CAATGCAGAA
    ACAGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC
    CTTGCAGCGC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TAAGGTCCCG GGCCAAAATT
    GAGAAAATTC GAGCAAGTTT ATTTAACAAC AATGACTTGA TAGGTTTGTC AAGCTTGGAT
    GGTGAGGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACGGTGTC TGTAGTGAAT
    CAGAGTCTAT TCGATACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA
    ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC
    TGTGCCCACT CGGAGCAGAT GCTGAGCCTG ACGCCAGCAA AGCCCATCAA AGTGTCGGAC
    ATTTATCTTA GCAAAGAGCA GATCAACTCC CAGACACCGG GCAACCTCCT CCACCTTTTC
    TTCACCAATG TCCGGCCCCC CAAAAAGGTG CTAGAGGACC AGCTCACCCA GATCCTGAGG
    AAGTATGGTG TGCCGAAGCC CAAGTTTGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG
    CAACACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCGCC CACCAAGGAC
    AAGGCAGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGTG AGAAGAAGCC CACCGTGAAA
    CCCAAGTCAC CCGAAAAGAG CAAGCCAGAT GAAAAGGACC CTGAAAAGTC ACCCACCAAG
    AAACAAGAAG TCCCAGAGGA GAAGTACCTG ACCTTAGAAG GATTTCACAA GTTTGTGATC
    GACCGAGCCA AGCAAGACAT CCGCAGCGTC TGGAGAGCGA TTTTATCCTG TGGCTACGAT
    CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCATG CGCACAAGGC CTCCAGGAAG
    TGGACCCTGG AGATGGATGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG
    GCCATCACGC CCGCGCGTCT GCATCCCCAC GAGGTGTATC TGGACCCTGC AGACGCCGCT
    GACCCCAGAG TGGCCTGTCT CTTGAACGTG CCCATCGAGA GCCTGCGCCT GCGCTTCGCT
    CTGCTGCAGT CCCTCAACAC CACGCTTGAG ACCTTCTTCC TGCCGCTGGT GGAGCTGCGC
    CAGACTCCCA TGTACACGCA CAGCATCGCT GCCCTGCTCA AGGAGGCCAA AGGGCTGATC
    TTTTATGACA CAAAGGTGAC TGTCATGAAT CGGGTGCTAA ATGCCACGGT GCAGAGGACG
    GCCGACCATG CGGCACCAGA GATCACTCTG GACCCACTGG AGATCGTGGG AGGGGAGATC
    AGAGCCTCTG AGAACTCCTA CTTCTGCCAG GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC
    TCTCAGCTTT GCGTCAAACT GGCGAGTGGT GGCGACCCTA CCTATGCCTT CAACATCCGC
    TTCNACGGGG AGGAGGTCCA TGGCACCAGT GGCTCCTTCC GCCACTTCCT GTGGCAGGTG
    TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC
    AACAAGAACA AGGGCAAGTA CATCCTGACC CCCAGCCCCA TCACCTATGG GGAGGAGCAG
    CTGCTGCACT TCCTGGGCCA GTTGCTAGGG ATCGCCATCA GAGCAGATGT CCCCCTCCCC
    CTGGACCTCC TGCCCTCCTT CTGGAAGACA CTGGTGGGCG AGCCCCTGGA CCCTGACCAA
    GACCTGCAGG AAGCAGACGT ACTCACCTAC AATTACGTCA AGAAATTTGA GAGTATCAAC
    GACGAGACGG AGTTGGAGGC CCTGTGTGCC GAGATCGCCT CCCAGCACCT GGCCACTGAG
    AGCCCCGAGG GGCCCAACAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GACCGGCGAG
    GAGGTGGAGC TGTGCAGCCG CGGCCGGCAC ATCCCCGTGG CGTGGGAGAA CAAGGACATC
    TATGCGGCCG CCATCCGGAG CTTGCGGCTG CGTGAGCTAC AGAACATGGA GTGTGTGACG
    GCCGTGCGCG CGGGCCTCGG GGCCATCATC CCCCTGCAGC TGCTTACCAC GCTCAGCCCA
    CTGGAGATGG AGCTGCGCAC CTGCGGCCTC CCATACATCA ACCTCGAGTT CCTGAAGGCC
    CACACCATGT ACCAGGTGGG GCTGATGGAG ACCGACCAGC ACATCGAGTT CTTCTGGGGG
    GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAG
    GAGCGCATCC CGTTCACCTG CCCCTGCAAA GATGGGGGTC CTGACACGGC CCATGTGCCC
    CCGTACCCCA TGAAGATTGC CCCCCCGGAT GGCACAGCAG GTTCCCCAGA TTCTCGCTAC
    ATCCGAGTGG AGACCTGTAT GTTCATGATC AAGCTTCCCC AGTACTCCTC GCTGGAAATC
    ATGCTGGAGA AACTACGTTG TGCCATTCAC TACCGCGAAG ACCCCCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.