CRB1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRB1
Entrez Gene ID	102394920
Full Name	crumbs 1, cell polarity complex component
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006064880.1	XP_006064942.1	protein crumbs homolog 1 isoform X2
XM_006064880.2	XP_006064942.2	protein crumbs homolog 1 isoform X2
XM_006064882.1	XP_006064944.1	protein crumbs homolog 1 isoform X4
XM_006064881.1	XP_006064943.1	protein crumbs homolog 1 isoform X3
XM_006064879.2	XP_006064941.2	protein crumbs homolog 1 isoform X1
XM_006064879.1	XP_006064941.1	protein crumbs homolog 1 isoform X1
XM_006064882.2	XP_006064944.2	protein crumbs homolog 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following CRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu20510	XM_006064879.1 Latest version!	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu20511	XM_006064880.1 Latest version!	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu20512	XM_006064881.1 Latest version!	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu20513	XM_006064882.1 Latest version!	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu65829	XM_006064879.2 Latest version!	Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu65830	XM_006064880.2 Latest version!	Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu65831	XM_006064882.2 Latest version!	Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRB1 ( XM_006064879.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064941.1 Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu20510
Clone ID Related Accession (Same CDS sequence)	XM_006064879.1
Accession Version	XM_006064879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein crumbs homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785151.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACC CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGGTGTGA CAAGGACATC 
GACGAGTGCG CGTCCGACCC CTGCCTGCAC GGCGGCCGGT GTCACGACCT GCTGAACGGG 
TTCCGGTGCG TGTGCGCGCT GGCCTTCGCA GGGCCGCGCT GCGAGCTGGA CTTGGCAGAT 
GACTTGCTGT CGGACATCTT CACCGCCATC GGCTCAGTGA CCTTAGCCTT GTTGCTGATC 
CTCTTTCTGG CAGTGGTGGT TTCTGTTCTC GCCTCCAACA GAAGGGCAAC CCAGGGCTCC 
TACAGCCCGA GCCGTCAGGA GAAGGACGGC TCTCGTGTGG AGATGTGGAG CGTGATGGGT 
GCCCCTGCAG CGGAGAGGCT GATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064941.1
CDS	205..4431
Translation	MALRTVNHRLLLCLSLSLLISIKSSFCNKNDTKCLSDSCQNNSTCKDFSKDKSCRCSDTAVHGDKDCREEEDPCLSSPCPGHASCVRVPGERRSRCRCPPGHSGAGCDTAAGPCGPHSCLHGGVCHQDSGPPVCVCPAGYAGRFCELDLDECASSPCLHGAVCQDRGDGYSCFCVPGYQGRHCDVEVDECVSEPCRNAATCLNEIGRYTCLCPRGFSGVNCELEVDECWSQPCLNGATCQDAAGAYFCDCAPGFLGDRCELNMDECASGPCLHGGLCVDGANRYSCTCAGSGFTGTRCETLLPPCWSEPCHNNATCEDSADRYPCRCRPGYTGAQCETDLDGCWSGPCQAGGRCVELSSETGRERLARLLSASRRPEASRYVCICPPGLTGVHCEEDVDECSSSPCRNGGTCENSPEGYTCHCPFDPHSGVFFGGRDCSDTLLGCADHPCLNNGTCVPHVHDGQHGFDCLCPPGYTGSRCGTVTTLSFLGSGFLRVSSGSALAQDSGCTVALSFQTIQPAALLLFRGDRDAFVMLEVLAGFVHLTIQVGDRPKVVLLIPHDTSDGGWHTVEATFAEAVTLALRDDSCMGTCVARAPSPFESDGSSACTLQNSFLGGLPEGRAHSGDALLNVYRIPSAPSLVGCLQDVHLDSNAITTENLSSDQSLNVRAGCARKDWCESQPCRDRGRCLDLWLGHQCECYRPYRGRGCHGEYVAGRFGQDDSPGYAVFHLNKDYEEDLTLSMFVRTRRPAGLLLALGNGTYQYLRVWLEHGRLAMLTPGSPKLLVTFVLSDGNVRLVSLKIKPNKIELYQSSQNLGFLSAPTWKLQRGDVLYVGGLPDRRETELSGGFFKGCIQDIRLNNENLEFFPNSTSNAAHSAVLVNVTEGCPGDNLCEPNPCHHGGVCYPLWDDFSCSCPANTAGKACQELRWCELGPCPPGAQCLQLPRGFECIANAVFNGQSHEIIFRSNGNITRELTNITFGFRTRDAHVVILHAEKEPEFLRIHIRDSRLLFQLRSGNSFYTLSLTSLQPVSDGVWYQVTISMTDPGAQASRWQMEVDGQTPPVTSAVAAGSLSFLKDDTDVYVGDRASDSTRALRGCLSTIAISGLYLSYFENARGLMHRPQEERFLKVSGPPVETGCLQLDACRSGPCLHGGRCEDAYSSHRCTCPAGRSGPHCELHVDECLSSPCIHGNCSAPGGAAYHCRCEPGFTGVNCESEVDHCQRHQCANGATCVSDANGYSCRCPGNFTGRFCRYLRLPSTVCGNEKTNLTCYNGGNCTEFQGEMKCACWPGFTGERCDKDIDECASDPCLHGGRCHDLLNGFRCVCALAFAGPRCELDLADDLLSDIFTAIGSVTLALLLILFLAVVVSVLASNRRATQGSYSPSRQEKDGSRVEMWSVMGAPAAERLI

Target ORF information:

RefSeq Version	XM_006064879.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064879.1

ORF Insert Sequence:

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACC CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGGTGTGA CAAGGACATC 
GACGAGTGCG CGTCCGACCC CTGCCTGCAC GGCGGCCGGT GTCACGACCT GCTGAACGGG 
TTCCGGTGCG TGTGCGCGCT GGCCTTCGCA GGGCCGCGCT GCGAGCTGGA CTTGGCAGAT 
GACTTGCTGT CGGACATCTT CACCGCCATC GGCTCAGTGA CCTTAGCCTT GTTGCTGATC 
CTCTTTCTGG CAGTGGTGGT TTCTGTTCTC GCCTCCAACA GAAGGGCAAC CCAGGGCTCC 
TACAGCCCGA GCCGTCAGGA GAAGGACGGC TCTCGTGTGG AGATGTGGAG CGTGATGGGT 
GCCCCTGCAG CGGAGAGGCT GATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB1 ( XM_006064880.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064942.1 Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu20511
Clone ID Related Accession (Same CDS sequence)	XM_006064880.1
Accession Version	XM_006064880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein crumbs homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785151.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGA CGGACCATCT GGTCCCGCAC CAGCACGGAA CACAGAACTC AGAGTGCACA 
GGTTCCTTTT GCAATAAAAA TGACACCAAG TGCCTCTCCG ATTCTTGCCA AAACAATTCT 
ACGTGCAAGG ATTTCTCAAA AGACAAGAGT TGCCGTTGTT CAGACACAGC CGTTCACGGG 
GACAAAGACT GTAGGGAGGA GGAGGACCCC TGCCTCTCCA GCCCCTGTCC TGGACACGCC 
TCGTGCGTCC GCGTCCCGGG GGAGAGGCGC TCTCGGTGCA GATGTCCTCC CGGGCACAGC 
GGGGCCGGCT GCGACACCGC CGCTGGCCCC TGCGGGCCCC ACTCCTGCCT GCACGGGGGC 
GTCTGCCACC AGGACTCTGG GCCCCCTGTG TGCGTCTGCC CCGCTGGCTA CGCGGGAAGG 
TTCTGCGAGC TGGACCTCGA TGAGTGCGCG TCTAGCCCCT GCCTGCACGG CGCCGTGTGC 
CAGGACAGAG GCGATGGCTA CTCCTGCTTC TGTGTCCCGG GCTATCAAGG CCGGCACTGC 
GACGTGGAAG TGGACGAGTG TGTGTCGGAG CCCTGCCGGA ACGCGGCCAC GTGCCTCAAC 
GAGATCGGAC GGTACACCTG CCTCTGCCCG CGCGGGTTCT CCGGTGTAAA CTGTGAACTG 
GAAGTTGATG AGTGTTGGTC CCAGCCCTGC CTCAATGGTG CCACTTGCCA GGACGCTGCG 
GGGGCCTACT TCTGTGACTG TGCCCCAGGA TTCCTGGGCG ATCGCTGTGA GCTCAACATG 
GACGAGTGTG CCAGTGGGCC ATGTCTCCAT GGCGGGCTGT GCGTGGATGG AGCCAACAGG 
TACAGCTGTA CCTGCGCAGG TAGTGGGTTC ACGGGGACAC GCTGTGAGAC CCTGCTGCCC 
CCGTGTTGGT CAGAGCCCTG CCACAACAAC GCCACCTGCG AGGACAGTGC TGACCGCTAC 
CCCTGCCGCT GCCGGCCCGG ATACACGGGC GCGCAGTGTG AGACCGACCT CGATGGATGC 
TGGAGCGGCC CCTGCCAGGC CGGCGGGCGG TGCGTGGAGC TGTCCTCGGA GACGGGGCGT 
GAGCGCCTGG CCCGGCTGCT GTCTGCATCC CGCCGCCCCG AAGCCTCCCG TTACGTCTGC 
ATCTGTCCAC CCGGACTCAC AGGAGTCCAC TGTGAAGAGG ACGTGGACGA GTGCTCGTCC 
AGCCCTTGCC GCAATGGGGG CACTTGTGAG AACTCGCCTG AGGGTTACAC TTGCCACTGC 
CCATTTGATC CCCATTCTGG AGTCTTCTTT GGAGGCAGGG ACTGCTCTGA TACTCTCCTG 
GGCTGTGCCG ATCATCCGTG TCTAAACAAC GGGACGTGCG TCCCTCACGT CCACGACGGC 
CAGCACGGAT TCGACTGCCT GTGCCCCCCT GGCTACACCG GGTCACGGTG TGGAACTGTC 
ACCACGCTGT CCTTTTTGGG CAGTGGCTTC CTGCGGGTCT CAAGTGGCTC AGCTCTGGCC 
CAGGACTCAG GCTGTACCGT AGCCCTCAGT TTCCAGACCA TCCAGCCAGC GGCCCTGCTG 
CTCTTCCGGG GTGACCGGGA CGCGTTTGTG ATGCTGGAGG TGCTGGCTGG CTTTGTCCAC 
TTGACCATTC AAGTTGGCGA TCGGCCGAAG GTGGTCCTGC TCATCCCCCA CGACACCAGC 
GACGGAGGGT GGCACACGGT GGAGGCGACG TTTGCAGAGG CTGTGACCCT CGCCCTACGC 
GACGACTCTT GCATGGGGAC ATGCGTTGCT AGGGCTCCTT CTCCATTTGA AAGTGATGGG 
TCATCAGCGT GTACTCTCCA GAACTCCTTT TTGGGGGGTT TGCCAGAAGG AAGAGCCCAC 
AGTGGTGATG CTCTGCTTAA CGTCTATAGG ATACCATCCG CACCTTCGCT CGTAGGCTGC 
CTCCAAGACG TGCACCTGGA CTCGAATGCC ATCACCACAG AGAACCTCTC ATCCGACCAG 
TCTCTGAACG TCAGGGCAGG CTGCGCCCGA AAAGACTGGT GTGAGAGCCA GCCGTGTCGC 
GACAGAGGAC GCTGCCTCGA CCTGTGGCTC GGCCACCAGT GTGAGTGCTA CAGGCCCTAC 
CGCGGCCGCG GCTGTCACGG AGAGTATGTG GCAGGCAGAT TTGGCCAAGA TGACTCTCCT 
GGATATGCTG TTTTTCATCT CAACAAGGAT TATGAAGAGG ACCTCACGCT CTCCATGTTT 
GTTCGAACAC GCCGTCCGGC CGGCTTGCTC TTAGCTCTGG GAAACGGCAC TTACCAGTAC 
CTCCGTGTCT GGCTAGAGCA TGGCAGGCTA GCGATGCTGA CTCCAGGTTC TCCCAAATTA 
TTAGTAACAT TTGTTCTCAG CGACGGAAAT GTCCGCTTAG TATCTTTGAA AATCAAGCCA 
AATAAAATTG AACTGTATCA GTCGTCACAA AATCTAGGAT TTCTTTCTGC TCCTACGTGG 
AAACTCCAAA GAGGAGATGT CCTCTATGTT GGTGGACTGC CTGACAGGCG AGAGACTGAG 
CTTAGTGGTG GATTCTTCAA AGGCTGTATC CAAGATATAA GGCTAAACAA CGAAAACCTG 
GAATTCTTCC CAAATTCAAC GAGCAACGCA GCCCACAGTG CCGTTCTTGT CAATGTGACC 
GAAGGCTGCC CCGGAGACAA CCTGTGTGAG CCCAACCCCT GTCACCACGG AGGCGTCTGC 
TACCCGCTGT GGGACGACTT CTCCTGCTCC TGCCCTGCGA ACACAGCGGG GAAGGCCTGC 
CAGGAGCTGC GCTGGTGTGA ACTCGGCCCG TGTCCTCCCG GCGCCCAGTG CCTGCAACTG 
CCTCGAGGCT TCGAATGTAT CGCAAATGCT GTTTTTAATG GACAAAGCCA TGAGATAATA 
TTCAGGAGCA ACGGGAACAT CACCAGAGAG CTCACCAACA TTACCTTCGG TTTCAGAACG 
AGGGATGCCC ACGTGGTCAT ACTGCACGCA GAGAAGGAGC CTGAATTCCT TCGTATTCAC 
ATTAGGGATT CCCGGTTGTT ATTTCAACTG CGAAGTGGCA ACAGCTTTTA CACGCTGAGT 
CTAACGAGTC TGCAGCCCGT CAGCGACGGC GTGTGGTACC AGGTGACCAT CTCCATGACA 
GACCCGGGGG CCCAGGCTTC CAGGTGGCAG ATGGAGGTGG ACGGGCAAAC GCCTCCTGTG 
ACCAGTGCAG TGGCTGCCGG GAGCCTCAGC TTCCTGAAGG ACGACACAGA CGTCTATGTG 
GGTGACCGAG CTAGCGACAG CACGCGGGCC CTGCGAGGGT GTCTAAGCAC GATAGCAATC 
AGCGGCCTCT ATCTCTCTTA CTTTGAAAAC GCCCGTGGCT TGATGCACAG ACCTCAGGAG 
GAGCGGTTTC TCAAAGTCTC CGGGCCTCCA GTGGAGACGG GCTGCCTGCA GCTCGACGCC 
TGCCGCTCCG GGCCCTGCCT GCACGGAGGA CGCTGTGAGG ACGCCTACAG CTCCCACCGC 
TGCACCTGTC CCGCGGGGCG GTCGGGGCCG CACTGCGAGC TCCACGTCGA TGAATGCCTT 
TCAAGCCCCT GTATCCACGG CAACTGCTCC GCCCCCGGCG GGGCAGCCTA TCACTGCAGG 
TGTGAGCCTG GCTTCACCGG TGTGAACTGC GAGTCGGAGG TGGACCACTG CCAGCGTCAC 
CAGTGTGCAA ACGGGGCCAC CTGCGTGAGT GACGCCAACG GCTACTCTTG CCGCTGTCCC 
GGAAATTTCA CGGGAAGGTT CTGCAGATAC CTCCGATTAC CCTCCACCGT CTGTGGGAAT 
GAGAAGACCA ACCTCACTTG CTACAACGGT GGCAACTGCA CGGAGTTCCA GGGAGAAATG 
AAATGCGCCT GCTGGCCAGG TTTTACGGGG GAACGGTGTG ACAAGGACAT CGACGAGTGC 
GCGTCCGACC CCTGCCTGCA CGGCGGCCGG TGTCACGACC TGCTGAACGG GTTCCGGTGC 
GTGTGCGCGC TGGCCTTCGC AGGGCCGCGC TGCGAGCTGG ACTTGGCAGA TGACTTGCTG 
TCGGACATCT TCACCGCCAT CGGCTCAGTG ACCTTAGCCT TGTTGCTGAT CCTCTTTCTG 
GCAGTGGTGG TTTCTGTTCT CGCCTCCAAC AGAAGGGCAA CCCAGGGCTC CTACAGCCCG 
AGCCGTCAGG AGAAGGACGG CTCTCGTGTG GAGATGTGGA GCGTGATGGG TGCCCCTGCA 
GCGGAGAGGC TGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064942.1
CDS	16..4233
Translation	MCLTDHLVPHQHGTQNSECTGSFCNKNDTKCLSDSCQNNSTCKDFSKDKSCRCSDTAVHGDKDCREEEDPCLSSPCPGHASCVRVPGERRSRCRCPPGHSGAGCDTAAGPCGPHSCLHGGVCHQDSGPPVCVCPAGYAGRFCELDLDECASSPCLHGAVCQDRGDGYSCFCVPGYQGRHCDVEVDECVSEPCRNAATCLNEIGRYTCLCPRGFSGVNCELEVDECWSQPCLNGATCQDAAGAYFCDCAPGFLGDRCELNMDECASGPCLHGGLCVDGANRYSCTCAGSGFTGTRCETLLPPCWSEPCHNNATCEDSADRYPCRCRPGYTGAQCETDLDGCWSGPCQAGGRCVELSSETGRERLARLLSASRRPEASRYVCICPPGLTGVHCEEDVDECSSSPCRNGGTCENSPEGYTCHCPFDPHSGVFFGGRDCSDTLLGCADHPCLNNGTCVPHVHDGQHGFDCLCPPGYTGSRCGTVTTLSFLGSGFLRVSSGSALAQDSGCTVALSFQTIQPAALLLFRGDRDAFVMLEVLAGFVHLTIQVGDRPKVVLLIPHDTSDGGWHTVEATFAEAVTLALRDDSCMGTCVARAPSPFESDGSSACTLQNSFLGGLPEGRAHSGDALLNVYRIPSAPSLVGCLQDVHLDSNAITTENLSSDQSLNVRAGCARKDWCESQPCRDRGRCLDLWLGHQCECYRPYRGRGCHGEYVAGRFGQDDSPGYAVFHLNKDYEEDLTLSMFVRTRRPAGLLLALGNGTYQYLRVWLEHGRLAMLTPGSPKLLVTFVLSDGNVRLVSLKIKPNKIELYQSSQNLGFLSAPTWKLQRGDVLYVGGLPDRRETELSGGFFKGCIQDIRLNNENLEFFPNSTSNAAHSAVLVNVTEGCPGDNLCEPNPCHHGGVCYPLWDDFSCSCPANTAGKACQELRWCELGPCPPGAQCLQLPRGFECIANAVFNGQSHEIIFRSNGNITRELTNITFGFRTRDAHVVILHAEKEPEFLRIHIRDSRLLFQLRSGNSFYTLSLTSLQPVSDGVWYQVTISMTDPGAQASRWQMEVDGQTPPVTSAVAAGSLSFLKDDTDVYVGDRASDSTRALRGCLSTIAISGLYLSYFENARGLMHRPQEERFLKVSGPPVETGCLQLDACRSGPCLHGGRCEDAYSSHRCTCPAGRSGPHCELHVDECLSSPCIHGNCSAPGGAAYHCRCEPGFTGVNCESEVDHCQRHQCANGATCVSDANGYSCRCPGNFTGRFCRYLRLPSTVCGNEKTNLTCYNGGNCTEFQGEMKCACWPGFTGERCDKDIDECASDPCLHGGRCHDLLNGFRCVCALAFAGPRCELDLADDLLSDIFTAIGSVTLALLLILFLAVVVSVLASNRRATQGSYSPSRQEKDGSRVEMWSVMGAPAAERLI

Target ORF information:

RefSeq Version	XM_006064880.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064880.1

ORF Insert Sequence:

ATGTGCTTGA CGGACCATCT GGTCCCGCAC CAGCACGGAA CACAGAACTC AGAGTGCACA 
GGTTCCTTTT GCAATAAAAA TGACACCAAG TGCCTCTCCG ATTCTTGCCA AAACAATTCT 
ACGTGCAAGG ATTTCTCAAA AGACAAGAGT TGCCGTTGTT CAGACACAGC CGTTCACGGG 
GACAAAGACT GTAGGGAGGA GGAGGACCCC TGCCTCTCCA GCCCCTGTCC TGGACACGCC 
TCGTGCGTCC GCGTCCCGGG GGAGAGGCGC TCTCGGTGCA GATGTCCTCC CGGGCACAGC 
GGGGCCGGCT GCGACACCGC CGCTGGCCCC TGCGGGCCCC ACTCCTGCCT GCACGGGGGC 
GTCTGCCACC AGGACTCTGG GCCCCCTGTG TGCGTCTGCC CCGCTGGCTA CGCGGGAAGG 
TTCTGCGAGC TGGACCTCGA TGAGTGCGCG TCTAGCCCCT GCCTGCACGG CGCCGTGTGC 
CAGGACAGAG GCGATGGCTA CTCCTGCTTC TGTGTCCCGG GCTATCAAGG CCGGCACTGC 
GACGTGGAAG TGGACGAGTG TGTGTCGGAG CCCTGCCGGA ACGCGGCCAC GTGCCTCAAC 
GAGATCGGAC GGTACACCTG CCTCTGCCCG CGCGGGTTCT CCGGTGTAAA CTGTGAACTG 
GAAGTTGATG AGTGTTGGTC CCAGCCCTGC CTCAATGGTG CCACTTGCCA GGACGCTGCG 
GGGGCCTACT TCTGTGACTG TGCCCCAGGA TTCCTGGGCG ATCGCTGTGA GCTCAACATG 
GACGAGTGTG CCAGTGGGCC ATGTCTCCAT GGCGGGCTGT GCGTGGATGG AGCCAACAGG 
TACAGCTGTA CCTGCGCAGG TAGTGGGTTC ACGGGGACAC GCTGTGAGAC CCTGCTGCCC 
CCGTGTTGGT CAGAGCCCTG CCACAACAAC GCCACCTGCG AGGACAGTGC TGACCGCTAC 
CCCTGCCGCT GCCGGCCCGG ATACACGGGC GCGCAGTGTG AGACCGACCT CGATGGATGC 
TGGAGCGGCC CCTGCCAGGC CGGCGGGCGG TGCGTGGAGC TGTCCTCGGA GACGGGGCGT 
GAGCGCCTGG CCCGGCTGCT GTCTGCATCC CGCCGCCCCG AAGCCTCCCG TTACGTCTGC 
ATCTGTCCAC CCGGACTCAC AGGAGTCCAC TGTGAAGAGG ACGTGGACGA GTGCTCGTCC 
AGCCCTTGCC GCAATGGGGG CACTTGTGAG AACTCGCCTG AGGGTTACAC TTGCCACTGC 
CCATTTGATC CCCATTCTGG AGTCTTCTTT GGAGGCAGGG ACTGCTCTGA TACTCTCCTG 
GGCTGTGCCG ATCATCCGTG TCTAAACAAC GGGACGTGCG TCCCTCACGT CCACGACGGC 
CAGCACGGAT TCGACTGCCT GTGCCCCCCT GGCTACACCG GGTCACGGTG TGGAACTGTC 
ACCACGCTGT CCTTTTTGGG CAGTGGCTTC CTGCGGGTCT CAAGTGGCTC AGCTCTGGCC 
CAGGACTCAG GCTGTACCGT AGCCCTCAGT TTCCAGACCA TCCAGCCAGC GGCCCTGCTG 
CTCTTCCGGG GTGACCGGGA CGCGTTTGTG ATGCTGGAGG TGCTGGCTGG CTTTGTCCAC 
TTGACCATTC AAGTTGGCGA TCGGCCGAAG GTGGTCCTGC TCATCCCCCA CGACACCAGC 
GACGGAGGGT GGCACACGGT GGAGGCGACG TTTGCAGAGG CTGTGACCCT CGCCCTACGC 
GACGACTCTT GCATGGGGAC ATGCGTTGCT AGGGCTCCTT CTCCATTTGA AAGTGATGGG 
TCATCAGCGT GTACTCTCCA GAACTCCTTT TTGGGGGGTT TGCCAGAAGG AAGAGCCCAC 
AGTGGTGATG CTCTGCTTAA CGTCTATAGG ATACCATCCG CACCTTCGCT CGTAGGCTGC 
CTCCAAGACG TGCACCTGGA CTCGAATGCC ATCACCACAG AGAACCTCTC ATCCGACCAG 
TCTCTGAACG TCAGGGCAGG CTGCGCCCGA AAAGACTGGT GTGAGAGCCA GCCGTGTCGC 
GACAGAGGAC GCTGCCTCGA CCTGTGGCTC GGCCACCAGT GTGAGTGCTA CAGGCCCTAC 
CGCGGCCGCG GCTGTCACGG AGAGTATGTG GCAGGCAGAT TTGGCCAAGA TGACTCTCCT 
GGATATGCTG TTTTTCATCT CAACAAGGAT TATGAAGAGG ACCTCACGCT CTCCATGTTT 
GTTCGAACAC GCCGTCCGGC CGGCTTGCTC TTAGCTCTGG GAAACGGCAC TTACCAGTAC 
CTCCGTGTCT GGCTAGAGCA TGGCAGGCTA GCGATGCTGA CTCCAGGTTC TCCCAAATTA 
TTAGTAACAT TTGTTCTCAG CGACGGAAAT GTCCGCTTAG TATCTTTGAA AATCAAGCCA 
AATAAAATTG AACTGTATCA GTCGTCACAA AATCTAGGAT TTCTTTCTGC TCCTACGTGG 
AAACTCCAAA GAGGAGATGT CCTCTATGTT GGTGGACTGC CTGACAGGCG AGAGACTGAG 
CTTAGTGGTG GATTCTTCAA AGGCTGTATC CAAGATATAA GGCTAAACAA CGAAAACCTG 
GAATTCTTCC CAAATTCAAC GAGCAACGCA GCCCACAGTG CCGTTCTTGT CAATGTGACC 
GAAGGCTGCC CCGGAGACAA CCTGTGTGAG CCCAACCCCT GTCACCACGG AGGCGTCTGC 
TACCCGCTGT GGGACGACTT CTCCTGCTCC TGCCCTGCGA ACACAGCGGG GAAGGCCTGC 
CAGGAGCTGC GCTGGTGTGA ACTCGGCCCG TGTCCTCCCG GCGCCCAGTG CCTGCAACTG 
CCTCGAGGCT TCGAATGTAT CGCAAATGCT GTTTTTAATG GACAAAGCCA TGAGATAATA 
TTCAGGAGCA ACGGGAACAT CACCAGAGAG CTCACCAACA TTACCTTCGG TTTCAGAACG 
AGGGATGCCC ACGTGGTCAT ACTGCACGCA GAGAAGGAGC CTGAATTCCT TCGTATTCAC 
ATTAGGGATT CCCGGTTGTT ATTTCAACTG CGAAGTGGCA ACAGCTTTTA CACGCTGAGT 
CTAACGAGTC TGCAGCCCGT CAGCGACGGC GTGTGGTACC AGGTGACCAT CTCCATGACA 
GACCCGGGGG CCCAGGCTTC CAGGTGGCAG ATGGAGGTGG ACGGGCAAAC GCCTCCTGTG 
ACCAGTGCAG TGGCTGCCGG GAGCCTCAGC TTCCTGAAGG ACGACACAGA CGTCTATGTG 
GGTGACCGAG CTAGCGACAG CACGCGGGCC CTGCGAGGGT GTCTAAGCAC GATAGCAATC 
AGCGGCCTCT ATCTCTCTTA CTTTGAAAAC GCCCGTGGCT TGATGCACAG ACCTCAGGAG 
GAGCGGTTTC TCAAAGTCTC CGGGCCTCCA GTGGAGACGG GCTGCCTGCA GCTCGACGCC 
TGCCGCTCCG GGCCCTGCCT GCACGGAGGA CGCTGTGAGG ACGCCTACAG CTCCCACCGC 
TGCACCTGTC CCGCGGGGCG GTCGGGGCCG CACTGCGAGC TCCACGTCGA TGAATGCCTT 
TCAAGCCCCT GTATCCACGG CAACTGCTCC GCCCCCGGCG GGGCAGCCTA TCACTGCAGG 
TGTGAGCCTG GCTTCACCGG TGTGAACTGC GAGTCGGAGG TGGACCACTG CCAGCGTCAC 
CAGTGTGCAA ACGGGGCCAC CTGCGTGAGT GACGCCAACG GCTACTCTTG CCGCTGTCCC 
GGAAATTTCA CGGGAAGGTT CTGCAGATAC CTCCGATTAC CCTCCACCGT CTGTGGGAAT 
GAGAAGACCA ACCTCACTTG CTACAACGGT GGCAACTGCA CGGAGTTCCA GGGAGAAATG 
AAATGCGCCT GCTGGCCAGG TTTTACGGGG GAACGGTGTG ACAAGGACAT CGACGAGTGC 
GCGTCCGACC CCTGCCTGCA CGGCGGCCGG TGTCACGACC TGCTGAACGG GTTCCGGTGC 
GTGTGCGCGC TGGCCTTCGC AGGGCCGCGC TGCGAGCTGG ACTTGGCAGA TGACTTGCTG 
TCGGACATCT TCACCGCCAT CGGCTCAGTG ACCTTAGCCT TGTTGCTGAT CCTCTTTCTG 
GCAGTGGTGG TTTCTGTTCT CGCCTCCAAC AGAAGGGCAA CCCAGGGCTC CTACAGCCCG 
AGCCGTCAGG AGAAGGACGG CTCTCGTGTG GAGATGTGGA GCGTGATGGG TGCCCCTGCA 
GCGGAGAGGC TGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB1 ( XM_006064881.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064943.1 Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X3, mRNA.

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CloneID	OBu20512
Clone ID Related Accession (Same CDS sequence)	XM_006064881.1
Accession Version	XM_006064881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein crumbs homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785151.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACC CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGGTGTGA CAAGGACATC 
GACGAGTGCG CGTCCGACCC CTGCCTGCAC GGCGGCCGGT GTCACGACCT GCTGAACGGG 
TTCCGGTGCG TGTGCGCGCT GGCCTTCGCA GGGCCGCGCT GCGAGCTGGA CATGGTGGTA 
CTGAGATGTC CATGCTGTGA AATCTGGAGT GAACATGCCA CCTGGAAACA GAGGCTGGGT 
TCTGCCAGGC TCCCAGCCAC CCTGCTGCTG GATGCAGCCA CACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064943.1
CDS	205..4332
Translation	MALRTVNHRLLLCLSLSLLISIKSSFCNKNDTKCLSDSCQNNSTCKDFSKDKSCRCSDTAVHGDKDCREEEDPCLSSPCPGHASCVRVPGERRSRCRCPPGHSGAGCDTAAGPCGPHSCLHGGVCHQDSGPPVCVCPAGYAGRFCELDLDECASSPCLHGAVCQDRGDGYSCFCVPGYQGRHCDVEVDECVSEPCRNAATCLNEIGRYTCLCPRGFSGVNCELEVDECWSQPCLNGATCQDAAGAYFCDCAPGFLGDRCELNMDECASGPCLHGGLCVDGANRYSCTCAGSGFTGTRCETLLPPCWSEPCHNNATCEDSADRYPCRCRPGYTGAQCETDLDGCWSGPCQAGGRCVELSSETGRERLARLLSASRRPEASRYVCICPPGLTGVHCEEDVDECSSSPCRNGGTCENSPEGYTCHCPFDPHSGVFFGGRDCSDTLLGCADHPCLNNGTCVPHVHDGQHGFDCLCPPGYTGSRCGTVTTLSFLGSGFLRVSSGSALAQDSGCTVALSFQTIQPAALLLFRGDRDAFVMLEVLAGFVHLTIQVGDRPKVVLLIPHDTSDGGWHTVEATFAEAVTLALRDDSCMGTCVARAPSPFESDGSSACTLQNSFLGGLPEGRAHSGDALLNVYRIPSAPSLVGCLQDVHLDSNAITTENLSSDQSLNVRAGCARKDWCESQPCRDRGRCLDLWLGHQCECYRPYRGRGCHGEYVAGRFGQDDSPGYAVFHLNKDYEEDLTLSMFVRTRRPAGLLLALGNGTYQYLRVWLEHGRLAMLTPGSPKLLVTFVLSDGNVRLVSLKIKPNKIELYQSSQNLGFLSAPTWKLQRGDVLYVGGLPDRRETELSGGFFKGCIQDIRLNNENLEFFPNSTSNAAHSAVLVNVTEGCPGDNLCEPNPCHHGGVCYPLWDDFSCSCPANTAGKACQELRWCELGPCPPGAQCLQLPRGFECIANAVFNGQSHEIIFRSNGNITRELTNITFGFRTRDAHVVILHAEKEPEFLRIHIRDSRLLFQLRSGNSFYTLSLTSLQPVSDGVWYQVTISMTDPGAQASRWQMEVDGQTPPVTSAVAAGSLSFLKDDTDVYVGDRASDSTRALRGCLSTIAISGLYLSYFENARGLMHRPQEERFLKVSGPPVETGCLQLDACRSGPCLHGGRCEDAYSSHRCTCPAGRSGPHCELHVDECLSSPCIHGNCSAPGGAAYHCRCEPGFTGVNCESEVDHCQRHQCANGATCVSDANGYSCRCPGNFTGRFCRYLRLPSTVCGNEKTNLTCYNGGNCTEFQGEMKCACWPGFTGERCDKDIDECASDPCLHGGRCHDLLNGFRCVCALAFAGPRCELDMVVLRCPCCEIWSEHATWKQRLGSARLPATLLLDAATR

Target ORF information:

RefSeq Version	XM_006064881.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064881.1

ORF Insert Sequence:

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACC CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGGTGTGA CAAGGACATC 
GACGAGTGCG CGTCCGACCC CTGCCTGCAC GGCGGCCGGT GTCACGACCT GCTGAACGGG 
TTCCGGTGCG TGTGCGCGCT GGCCTTCGCA GGGCCGCGCT GCGAGCTGGA CATGGTGGTA 
CTGAGATGTC CATGCTGTGA AATCTGGAGT GAACATGCCA CCTGGAAACA GAGGCTGGGT 
TCTGCCAGGC TCCCAGCCAC CCTGCTGCTG GATGCAGCCA CACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB1 ( XM_006064882.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064944.1 Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu20513
Clone ID Related Accession (Same CDS sequence)	XM_006064882.1
Accession Version	XM_006064882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein crumbs homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785151.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACC CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGTTGGCA GATGACTTGC 
TGTCGGACAT CTTCACCGCC ATCGGCTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064944.1
CDS	205..4137
Translation	MALRTVNHRLLLCLSLSLLISIKSSFCNKNDTKCLSDSCQNNSTCKDFSKDKSCRCSDTAVHGDKDCREEEDPCLSSPCPGHASCVRVPGERRSRCRCPPGHSGAGCDTAAGPCGPHSCLHGGVCHQDSGPPVCVCPAGYAGRFCELDLDECASSPCLHGAVCQDRGDGYSCFCVPGYQGRHCDVEVDECVSEPCRNAATCLNEIGRYTCLCPRGFSGVNCELEVDECWSQPCLNGATCQDAAGAYFCDCAPGFLGDRCELNMDECASGPCLHGGLCVDGANRYSCTCAGSGFTGTRCETLLPPCWSEPCHNNATCEDSADRYPCRCRPGYTGAQCETDLDGCWSGPCQAGGRCVELSSETGRERLARLLSASRRPEASRYVCICPPGLTGVHCEEDVDECSSSPCRNGGTCENSPEGYTCHCPFDPHSGVFFGGRDCSDTLLGCADHPCLNNGTCVPHVHDGQHGFDCLCPPGYTGSRCGTVTTLSFLGSGFLRVSSGSALAQDSGCTVALSFQTIQPAALLLFRGDRDAFVMLEVLAGFVHLTIQVGDRPKVVLLIPHDTSDGGWHTVEATFAEAVTLALRDDSCMGTCVARAPSPFESDGSSACTLQNSFLGGLPEGRAHSGDALLNVYRIPSAPSLVGCLQDVHLDSNAITTENLSSDQSLNVRAGCARKDWCESQPCRDRGRCLDLWLGHQCECYRPYRGRGCHGEYVAGRFGQDDSPGYAVFHLNKDYEEDLTLSMFVRTRRPAGLLLALGNGTYQYLRVWLEHGRLAMLTPGSPKLLVTFVLSDGNVRLVSLKIKPNKIELYQSSQNLGFLSAPTWKLQRGDVLYVGGLPDRRETELSGGFFKGCIQDIRLNNENLEFFPNSTSNAAHSAVLVNVTEGCPGDNLCEPNPCHHGGVCYPLWDDFSCSCPANTAGKACQELRWCELGPCPPGAQCLQLPRGFECIANAVFNGQSHEIIFRSNGNITRELTNITFGFRTRDAHVVILHAEKEPEFLRIHIRDSRLLFQLRSGNSFYTLSLTSLQPVSDGVWYQVTISMTDPGAQASRWQMEVDGQTPPVTSAVAAGSLSFLKDDTDVYVGDRASDSTRALRGCLSTIAISGLYLSYFENARGLMHRPQEERFLKVSGPPVETGCLQLDACRSGPCLHGGRCEDAYSSHRCTCPAGRSGPHCELHVDECLSSPCIHGNCSAPGGAAYHCRCEPGFTGVNCESEVDHCQRHQCANGATCVSDANGYSCRCPGNFTGRFCRYLRLPSTVCGNEKTNLTCYNGGNCTEFQGEMKCACWPGFTGERWQMTCCRTSSPPSAQ

Target ORF information:

RefSeq Version	XM_006064882.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis crumbs homolog 1 (Drosophila) (CRB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064882.1

ORF Insert Sequence:

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACC CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGTTGGCA GATGACTTGC 
TGTCGGACAT CTTCACCGCC ATCGGCTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB1 ( XM_006064879.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064941.2 Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu65829
Clone ID Related Accession (Same CDS sequence)	XM_006064879.2
Accession Version	XM_006064879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein crumbs homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064879.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACA CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGGTGTGA CAAGGACATC 
GACGAGTGCG CGTCCGACCC CTGCCTGCAC GGCGGCCGGT GTCACGACCT GCTGAACGGG 
TTCCGGTGCG TGTGCGCGCT GGCCTTCGCA GGGCCGCGCT GCGAGCTGGA CTTGGCAGAT 
GACTTGCTGT CGGACATCTT CACCGCCATC GGCTCAGTGA CCTTAGCCTT GTTGCTGATC 
CTCTTTCTGG CAGTGGTGGT TTCTGTTCTC GCCTCCAACA GAAGGGCAAC CCAGGGCTCC 
TACAGCCCGA GCCGTCAGGA GAAGGACGGC TCTCGTGTGG AGATGTGGAG CGTGATGGGT 
GCCCCTGCAG CGGAGAGGCT GATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064941.2
CDS	454..4680
Translation	MALRTVNHRLLLCLSLSLLISIKSSFCNKNDTKCLSDSCQNNSTCKDFSKDKSCRCSDTAVHGDKDCREEEDPCLSSPCPGHASCVRVPGERRSRCRCPPGHSGAGCDTAAGPCGPHSCLHGGVCHQDSGPPVCVCPAGYAGRFCELDLDECASSPCLHGAVCQDRGDGYSCFCVPGYQGRHCDVEVDECVSEPCRNAATCLNEIGRYTCLCPRGFSGVNCELEVDECWSQPCLNGATCQDAAGAYFCDCAPGFLGDRCELNMDECASGPCLHGGLCVDGANRYSCTCAGSGFTGTRCETLLPPCWSEPCHNNATCEDSADRYTCRCRPGYTGAQCETDLDGCWSGPCQAGGRCVELSSETGRERLARLLSASRRPEASRYVCICPPGLTGVHCEEDVDECSSSPCRNGGTCENSPEGYTCHCPFDPHSGVFFGGRDCSDTLLGCADHPCLNNGTCVPHVHDGQHGFDCLCPPGYTGSRCGTVTTLSFLGSGFLRVSSGSALAQDSGCTVALSFQTIQPAALLLFRGDRDAFVMLEVLAGFVHLTIQVGDRPKVVLLIPHDTSDGGWHTVEATFAEAVTLALRDDSCMGTCVARAPSPFESDGSSACTLQNSFLGGLPEGRAHSGDALLNVYRIPSAPSLVGCLQDVHLDSNAITTENLSSDQSLNVRAGCARKDWCESQPCRDRGRCLDLWLGHQCECYRPYRGRGCHGEYVAGRFGQDDSPGYAVFHLNKDYEEDLTLSMFVRTRRPAGLLLALGNGTYQYLRVWLEHGRLAMLTPGSPKLLVTFVLSDGNVRLVSLKIKPNKIELYQSSQNLGFLSAPTWKLQRGDVLYVGGLPDRRETELSGGFFKGCIQDIRLNNENLEFFPNSTSNAAHSAVLVNVTEGCPGDNLCEPNPCHHGGVCYPLWDDFSCSCPANTAGKACQELRWCELGPCPPGAQCLQLPRGFECIANAVFNGQSHEIIFRSNGNITRELTNITFGFRTRDAHVVILHAEKEPEFLRIHIRDSRLLFQLRSGNSFYTLSLTSLQPVSDGVWYQVTISMTDPGAQASRWQMEVDGQTPPVTSAVAAGSLSFLKDDTDVYVGDRASDSTRALRGCLSTIAISGLYLSYFENARGLMHRPQEERFLKVSGPPVETGCLQLDACRSGPCLHGGRCEDAYSSHRCTCPAGRSGPHCELHVDECLSSPCIHGNCSAPGGAAYHCRCEPGFTGVNCESEVDHCQRHQCANGATCVSDANGYSCRCPGNFTGRFCRYLRLPSTVCGNEKTNLTCYNGGNCTEFQGEMKCACWPGFTGERCDKDIDECASDPCLHGGRCHDLLNGFRCVCALAFAGPRCELDLADDLLSDIFTAIGSVTLALLLILFLAVVVSVLASNRRATQGSYSPSRQEKDGSRVEMWSVMGAPAAERLI

Target ORF information:

RefSeq Version	XM_006064879.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064879.2

ORF Insert Sequence:

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACA CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGGTGTGA CAAGGACATC 
GACGAGTGCG CGTCCGACCC CTGCCTGCAC GGCGGCCGGT GTCACGACCT GCTGAACGGG 
TTCCGGTGCG TGTGCGCGCT GGCCTTCGCA GGGCCGCGCT GCGAGCTGGA CTTGGCAGAT 
GACTTGCTGT CGGACATCTT CACCGCCATC GGCTCAGTGA CCTTAGCCTT GTTGCTGATC 
CTCTTTCTGG CAGTGGTGGT TTCTGTTCTC GCCTCCAACA GAAGGGCAAC CCAGGGCTCC 
TACAGCCCGA GCCGTCAGGA GAAGGACGGC TCTCGTGTGG AGATGTGGAG CGTGATGGGT 
GCCCCTGCAG CGGAGAGGCT GATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB1 ( XM_006064880.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064942.2 Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X2, mRNA.

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CloneID	OBu65830
Clone ID Related Accession (Same CDS sequence)	XM_006064880.2
Accession Version	XM_006064880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein crumbs homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGA CGGACCATCT GGTCCCGCAC CAGCACGGAA CACAGAACTC AGAGTGCACA 
GGTTCCTTTT GCAATAAAAA TGACACCAAG TGCCTCTCCG ATTCTTGCCA AAACAATTCT 
ACGTGCAAGG ATTTCTCAAA AGACAAGAGT TGCCGTTGTT CAGACACAGC CGTTCACGGG 
GACAAAGACT GTAGGGAGGA GGAGGACCCC TGCCTCTCCA GCCCCTGTCC TGGACACGCC 
TCGTGCGTCC GCGTCCCGGG GGAGAGGCGC TCTCGGTGCA GATGTCCTCC CGGGCACAGC 
GGGGCCGGCT GCGACACCGC CGCTGGCCCC TGCGGGCCCC ACTCCTGCCT GCACGGGGGC 
GTCTGCCACC AGGACTCTGG GCCCCCTGTG TGCGTCTGCC CCGCTGGCTA CGCGGGAAGG 
TTCTGCGAGC TGGACCTCGA TGAGTGCGCG TCTAGCCCCT GCCTGCACGG CGCCGTGTGC 
CAGGACAGAG GCGATGGCTA CTCCTGCTTC TGTGTCCCGG GCTATCAAGG CCGGCACTGC 
GACGTGGAAG TGGACGAGTG TGTGTCGGAG CCCTGCCGGA ACGCGGCCAC GTGCCTCAAC 
GAGATCGGAC GGTACACCTG CCTCTGCCCG CGCGGGTTCT CCGGTGTAAA CTGTGAACTG 
GAAGTTGATG AGTGTTGGTC CCAGCCCTGC CTCAATGGTG CCACTTGCCA GGACGCTGCG 
GGGGCCTACT TCTGTGACTG TGCCCCAGGA TTCCTGGGCG ATCGCTGTGA GCTCAACATG 
GACGAGTGTG CCAGTGGGCC ATGTCTCCAT GGCGGGCTGT GCGTGGATGG AGCCAACAGG 
TACAGCTGTA CCTGCGCAGG TAGTGGGTTC ACGGGGACAC GCTGTGAGAC CCTGCTGCCC 
CCGTGTTGGT CAGAGCCCTG CCACAACAAC GCCACCTGCG AGGACAGTGC TGACCGCTAC 
ACCTGCCGCT GCCGGCCCGG ATACACGGGC GCGCAGTGTG AGACCGACCT CGATGGATGC 
TGGAGCGGCC CCTGCCAGGC CGGCGGGCGG TGCGTGGAGC TGTCCTCGGA GACGGGGCGT 
GAGCGCCTGG CCCGGCTGCT GTCTGCATCC CGCCGCCCCG AAGCCTCCCG TTACGTCTGC 
ATCTGTCCAC CCGGACTCAC AGGAGTCCAC TGTGAAGAGG ACGTGGACGA GTGCTCGTCC 
AGCCCTTGCC GCAATGGGGG CACTTGTGAG AACTCGCCTG AGGGTTACAC TTGCCACTGC 
CCATTTGATC CCCATTCTGG AGTCTTCTTT GGAGGCAGGG ACTGCTCTGA TACTCTCCTG 
GGCTGTGCCG ATCATCCGTG TCTAAACAAC GGGACGTGCG TCCCTCACGT CCACGACGGC 
CAGCACGGAT TCGACTGCCT GTGCCCCCCT GGCTACACCG GGTCACGGTG TGGAACTGTC 
ACCACGCTGT CCTTTTTGGG CAGTGGCTTC CTGCGGGTCT CAAGTGGCTC AGCTCTGGCC 
CAGGACTCAG GCTGTACCGT AGCCCTCAGT TTCCAGACCA TCCAGCCAGC GGCCCTGCTG 
CTCTTCCGGG GTGACCGGGA CGCGTTTGTG ATGCTGGAGG TGCTGGCTGG CTTTGTCCAC 
TTGACCATTC AAGTTGGCGA TCGGCCGAAG GTGGTCCTGC TCATCCCCCA CGACACCAGC 
GACGGAGGGT GGCACACGGT GGAGGCGACG TTTGCAGAGG CTGTGACCCT CGCCCTACGC 
GACGACTCTT GCATGGGGAC ATGCGTTGCT AGGGCTCCTT CTCCATTTGA AAGTGATGGG 
TCATCAGCGT GTACTCTCCA GAACTCCTTT TTGGGGGGTT TGCCAGAAGG AAGAGCCCAC 
AGTGGTGATG CTCTGCTTAA CGTCTATAGG ATACCATCCG CACCTTCGCT CGTAGGCTGC 
CTCCAAGACG TGCACCTGGA CTCGAATGCC ATCACCACAG AGAACCTCTC ATCCGACCAG 
TCTCTGAACG TCAGGGCAGG CTGCGCCCGA AAAGACTGGT GTGAGAGCCA GCCGTGTCGC 
GACAGAGGAC GCTGCCTCGA CCTGTGGCTC GGCCACCAGT GTGAGTGCTA CAGGCCCTAC 
CGCGGCCGCG GCTGTCACGG AGAGTATGTG GCAGGCAGAT TTGGCCAAGA TGACTCTCCT 
GGATATGCTG TTTTTCATCT CAACAAGGAT TATGAAGAGG ACCTCACGCT CTCCATGTTT 
GTTCGAACAC GCCGTCCGGC CGGCTTGCTC TTAGCTCTGG GAAACGGCAC TTACCAGTAC 
CTCCGTGTCT GGCTAGAGCA TGGCAGGCTA GCGATGCTGA CTCCAGGTTC TCCCAAATTA 
TTAGTAACAT TTGTTCTCAG CGACGGAAAT GTCCGCTTAG TATCTTTGAA AATCAAGCCA 
AATAAAATTG AACTGTATCA GTCGTCACAA AATCTAGGAT TTCTTTCTGC TCCTACGTGG 
AAACTCCAAA GAGGAGATGT CCTCTATGTT GGTGGACTGC CTGACAGGCG AGAGACTGAG 
CTTAGTGGTG GATTCTTCAA AGGCTGTATC CAAGATATAA GGCTAAACAA CGAAAACCTG 
GAATTCTTCC CAAATTCAAC GAGCAACGCA GCCCACAGTG CCGTTCTTGT CAATGTGACC 
GAAGGCTGCC CCGGAGACAA CCTGTGTGAG CCCAACCCCT GTCACCACGG AGGCGTCTGC 
TACCCGCTGT GGGACGACTT CTCCTGCTCC TGCCCTGCGA ACACAGCGGG GAAGGCCTGC 
CAGGAGCTGC GCTGGTGTGA ACTCGGCCCG TGTCCTCCCG GCGCCCAGTG CCTGCAACTG 
CCTCGAGGCT TCGAATGTAT CGCAAATGCT GTTTTTAATG GACAAAGCCA TGAGATAATA 
TTCAGGAGCA ACGGGAACAT CACCAGAGAG CTCACCAACA TTACCTTCGG TTTCAGAACG 
AGGGATGCCC ACGTGGTCAT ACTGCACGCA GAGAAGGAGC CTGAATTCCT TCGTATTCAC 
ATTAGGGATT CCCGGTTGTT ATTTCAACTG CGAAGTGGCA ACAGCTTTTA CACGCTGAGT 
CTAACGAGTC TGCAGCCCGT CAGCGACGGC GTGTGGTACC AGGTGACCAT CTCCATGACA 
GACCCGGGGG CCCAGGCTTC CAGGTGGCAG ATGGAGGTGG ACGGGCAAAC GCCTCCTGTG 
ACCAGTGCAG TGGCTGCCGG GAGCCTCAGC TTCCTGAAGG ACGACACAGA CGTCTATGTG 
GGTGACCGAG CTAGCGACAG CACGCGGGCC CTGCGAGGGT GTCTAAGCAC GATAGCAATC 
AGCGGCCTCT ATCTCTCTTA CTTTGAAAAC GCCCGTGGCT TGATGCACAG ACCTCAGGAG 
GAGCGGTTTC TCAAAGTCTC CGGGCCTCCA GTGGAGACGG GCTGCCTGCA GCTCGACGCC 
TGCCGCTCCG GGCCCTGCCT GCACGGAGGA CGCTGTGAGG ACGCCTACAG CTCCCACCGC 
TGCACCTGTC CCGCGGGGCG GTCGGGGCCG CACTGCGAGC TCCACGTCGA TGAATGCCTT 
TCAAGCCCCT GTATCCACGG CAACTGCTCC GCCCCCGGCG GGGCAGCCTA TCACTGCAGG 
TGTGAGCCTG GCTTCACCGG TGTGAACTGC GAGTCGGAGG TGGACCACTG CCAGCGTCAC 
CAGTGTGCAA ACGGGGCCAC CTGCGTGAGT GACGCCAACG GCTACTCTTG CCGCTGTCCC 
GGAAATTTCA CGGGAAGGTT CTGCAGATAC CTCCGATTAC CCTCCACCGT CTGTGGGAAT 
GAGAAGACCA ACCTCACTTG CTACAACGGT GGCAACTGCA CGGAGTTCCA GGGAGAAATG 
AAATGCGCCT GCTGGCCAGG TTTTACGGGG GAACGGTGTG ACAAGGACAT CGACGAGTGC 
GCGTCCGACC CCTGCCTGCA CGGCGGCCGG TGTCACGACC TGCTGAACGG GTTCCGGTGC 
GTGTGCGCGC TGGCCTTCGC AGGGCCGCGC TGCGAGCTGG ACTTGGCAGA TGACTTGCTG 
TCGGACATCT TCACCGCCAT CGGCTCAGTG ACCTTAGCCT TGTTGCTGAT CCTCTTTCTG 
GCAGTGGTGG TTTCTGTTCT CGCCTCCAAC AGAAGGGCAA CCCAGGGCTC CTACAGCCCG 
AGCCGTCAGG AGAAGGACGG CTCTCGTGTG GAGATGTGGA GCGTGATGGG TGCCCCTGCA 
GCGGAGAGGC TGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064942.2
CDS	99..4316
Translation	MCLTDHLVPHQHGTQNSECTGSFCNKNDTKCLSDSCQNNSTCKDFSKDKSCRCSDTAVHGDKDCREEEDPCLSSPCPGHASCVRVPGERRSRCRCPPGHSGAGCDTAAGPCGPHSCLHGGVCHQDSGPPVCVCPAGYAGRFCELDLDECASSPCLHGAVCQDRGDGYSCFCVPGYQGRHCDVEVDECVSEPCRNAATCLNEIGRYTCLCPRGFSGVNCELEVDECWSQPCLNGATCQDAAGAYFCDCAPGFLGDRCELNMDECASGPCLHGGLCVDGANRYSCTCAGSGFTGTRCETLLPPCWSEPCHNNATCEDSADRYTCRCRPGYTGAQCETDLDGCWSGPCQAGGRCVELSSETGRERLARLLSASRRPEASRYVCICPPGLTGVHCEEDVDECSSSPCRNGGTCENSPEGYTCHCPFDPHSGVFFGGRDCSDTLLGCADHPCLNNGTCVPHVHDGQHGFDCLCPPGYTGSRCGTVTTLSFLGSGFLRVSSGSALAQDSGCTVALSFQTIQPAALLLFRGDRDAFVMLEVLAGFVHLTIQVGDRPKVVLLIPHDTSDGGWHTVEATFAEAVTLALRDDSCMGTCVARAPSPFESDGSSACTLQNSFLGGLPEGRAHSGDALLNVYRIPSAPSLVGCLQDVHLDSNAITTENLSSDQSLNVRAGCARKDWCESQPCRDRGRCLDLWLGHQCECYRPYRGRGCHGEYVAGRFGQDDSPGYAVFHLNKDYEEDLTLSMFVRTRRPAGLLLALGNGTYQYLRVWLEHGRLAMLTPGSPKLLVTFVLSDGNVRLVSLKIKPNKIELYQSSQNLGFLSAPTWKLQRGDVLYVGGLPDRRETELSGGFFKGCIQDIRLNNENLEFFPNSTSNAAHSAVLVNVTEGCPGDNLCEPNPCHHGGVCYPLWDDFSCSCPANTAGKACQELRWCELGPCPPGAQCLQLPRGFECIANAVFNGQSHEIIFRSNGNITRELTNITFGFRTRDAHVVILHAEKEPEFLRIHIRDSRLLFQLRSGNSFYTLSLTSLQPVSDGVWYQVTISMTDPGAQASRWQMEVDGQTPPVTSAVAAGSLSFLKDDTDVYVGDRASDSTRALRGCLSTIAISGLYLSYFENARGLMHRPQEERFLKVSGPPVETGCLQLDACRSGPCLHGGRCEDAYSSHRCTCPAGRSGPHCELHVDECLSSPCIHGNCSAPGGAAYHCRCEPGFTGVNCESEVDHCQRHQCANGATCVSDANGYSCRCPGNFTGRFCRYLRLPSTVCGNEKTNLTCYNGGNCTEFQGEMKCACWPGFTGERCDKDIDECASDPCLHGGRCHDLLNGFRCVCALAFAGPRCELDLADDLLSDIFTAIGSVTLALLLILFLAVVVSVLASNRRATQGSYSPSRQEKDGSRVEMWSVMGAPAAERLI

Target ORF information:

RefSeq Version	XM_006064880.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064880.2

ORF Insert Sequence:

ATGTGCTTGA CGGACCATCT GGTCCCGCAC CAGCACGGAA CACAGAACTC AGAGTGCACA 
GGTTCCTTTT GCAATAAAAA TGACACCAAG TGCCTCTCCG ATTCTTGCCA AAACAATTCT 
ACGTGCAAGG ATTTCTCAAA AGACAAGAGT TGCCGTTGTT CAGACACAGC CGTTCACGGG 
GACAAAGACT GTAGGGAGGA GGAGGACCCC TGCCTCTCCA GCCCCTGTCC TGGACACGCC 
TCGTGCGTCC GCGTCCCGGG GGAGAGGCGC TCTCGGTGCA GATGTCCTCC CGGGCACAGC 
GGGGCCGGCT GCGACACCGC CGCTGGCCCC TGCGGGCCCC ACTCCTGCCT GCACGGGGGC 
GTCTGCCACC AGGACTCTGG GCCCCCTGTG TGCGTCTGCC CCGCTGGCTA CGCGGGAAGG 
TTCTGCGAGC TGGACCTCGA TGAGTGCGCG TCTAGCCCCT GCCTGCACGG CGCCGTGTGC 
CAGGACAGAG GCGATGGCTA CTCCTGCTTC TGTGTCCCGG GCTATCAAGG CCGGCACTGC 
GACGTGGAAG TGGACGAGTG TGTGTCGGAG CCCTGCCGGA ACGCGGCCAC GTGCCTCAAC 
GAGATCGGAC GGTACACCTG CCTCTGCCCG CGCGGGTTCT CCGGTGTAAA CTGTGAACTG 
GAAGTTGATG AGTGTTGGTC CCAGCCCTGC CTCAATGGTG CCACTTGCCA GGACGCTGCG 
GGGGCCTACT TCTGTGACTG TGCCCCAGGA TTCCTGGGCG ATCGCTGTGA GCTCAACATG 
GACGAGTGTG CCAGTGGGCC ATGTCTCCAT GGCGGGCTGT GCGTGGATGG AGCCAACAGG 
TACAGCTGTA CCTGCGCAGG TAGTGGGTTC ACGGGGACAC GCTGTGAGAC CCTGCTGCCC 
CCGTGTTGGT CAGAGCCCTG CCACAACAAC GCCACCTGCG AGGACAGTGC TGACCGCTAC 
ACCTGCCGCT GCCGGCCCGG ATACACGGGC GCGCAGTGTG AGACCGACCT CGATGGATGC 
TGGAGCGGCC CCTGCCAGGC CGGCGGGCGG TGCGTGGAGC TGTCCTCGGA GACGGGGCGT 
GAGCGCCTGG CCCGGCTGCT GTCTGCATCC CGCCGCCCCG AAGCCTCCCG TTACGTCTGC 
ATCTGTCCAC CCGGACTCAC AGGAGTCCAC TGTGAAGAGG ACGTGGACGA GTGCTCGTCC 
AGCCCTTGCC GCAATGGGGG CACTTGTGAG AACTCGCCTG AGGGTTACAC TTGCCACTGC 
CCATTTGATC CCCATTCTGG AGTCTTCTTT GGAGGCAGGG ACTGCTCTGA TACTCTCCTG 
GGCTGTGCCG ATCATCCGTG TCTAAACAAC GGGACGTGCG TCCCTCACGT CCACGACGGC 
CAGCACGGAT TCGACTGCCT GTGCCCCCCT GGCTACACCG GGTCACGGTG TGGAACTGTC 
ACCACGCTGT CCTTTTTGGG CAGTGGCTTC CTGCGGGTCT CAAGTGGCTC AGCTCTGGCC 
CAGGACTCAG GCTGTACCGT AGCCCTCAGT TTCCAGACCA TCCAGCCAGC GGCCCTGCTG 
CTCTTCCGGG GTGACCGGGA CGCGTTTGTG ATGCTGGAGG TGCTGGCTGG CTTTGTCCAC 
TTGACCATTC AAGTTGGCGA TCGGCCGAAG GTGGTCCTGC TCATCCCCCA CGACACCAGC 
GACGGAGGGT GGCACACGGT GGAGGCGACG TTTGCAGAGG CTGTGACCCT CGCCCTACGC 
GACGACTCTT GCATGGGGAC ATGCGTTGCT AGGGCTCCTT CTCCATTTGA AAGTGATGGG 
TCATCAGCGT GTACTCTCCA GAACTCCTTT TTGGGGGGTT TGCCAGAAGG AAGAGCCCAC 
AGTGGTGATG CTCTGCTTAA CGTCTATAGG ATACCATCCG CACCTTCGCT CGTAGGCTGC 
CTCCAAGACG TGCACCTGGA CTCGAATGCC ATCACCACAG AGAACCTCTC ATCCGACCAG 
TCTCTGAACG TCAGGGCAGG CTGCGCCCGA AAAGACTGGT GTGAGAGCCA GCCGTGTCGC 
GACAGAGGAC GCTGCCTCGA CCTGTGGCTC GGCCACCAGT GTGAGTGCTA CAGGCCCTAC 
CGCGGCCGCG GCTGTCACGG AGAGTATGTG GCAGGCAGAT TTGGCCAAGA TGACTCTCCT 
GGATATGCTG TTTTTCATCT CAACAAGGAT TATGAAGAGG ACCTCACGCT CTCCATGTTT 
GTTCGAACAC GCCGTCCGGC CGGCTTGCTC TTAGCTCTGG GAAACGGCAC TTACCAGTAC 
CTCCGTGTCT GGCTAGAGCA TGGCAGGCTA GCGATGCTGA CTCCAGGTTC TCCCAAATTA 
TTAGTAACAT TTGTTCTCAG CGACGGAAAT GTCCGCTTAG TATCTTTGAA AATCAAGCCA 
AATAAAATTG AACTGTATCA GTCGTCACAA AATCTAGGAT TTCTTTCTGC TCCTACGTGG 
AAACTCCAAA GAGGAGATGT CCTCTATGTT GGTGGACTGC CTGACAGGCG AGAGACTGAG 
CTTAGTGGTG GATTCTTCAA AGGCTGTATC CAAGATATAA GGCTAAACAA CGAAAACCTG 
GAATTCTTCC CAAATTCAAC GAGCAACGCA GCCCACAGTG CCGTTCTTGT CAATGTGACC 
GAAGGCTGCC CCGGAGACAA CCTGTGTGAG CCCAACCCCT GTCACCACGG AGGCGTCTGC 
TACCCGCTGT GGGACGACTT CTCCTGCTCC TGCCCTGCGA ACACAGCGGG GAAGGCCTGC 
CAGGAGCTGC GCTGGTGTGA ACTCGGCCCG TGTCCTCCCG GCGCCCAGTG CCTGCAACTG 
CCTCGAGGCT TCGAATGTAT CGCAAATGCT GTTTTTAATG GACAAAGCCA TGAGATAATA 
TTCAGGAGCA ACGGGAACAT CACCAGAGAG CTCACCAACA TTACCTTCGG TTTCAGAACG 
AGGGATGCCC ACGTGGTCAT ACTGCACGCA GAGAAGGAGC CTGAATTCCT TCGTATTCAC 
ATTAGGGATT CCCGGTTGTT ATTTCAACTG CGAAGTGGCA ACAGCTTTTA CACGCTGAGT 
CTAACGAGTC TGCAGCCCGT CAGCGACGGC GTGTGGTACC AGGTGACCAT CTCCATGACA 
GACCCGGGGG CCCAGGCTTC CAGGTGGCAG ATGGAGGTGG ACGGGCAAAC GCCTCCTGTG 
ACCAGTGCAG TGGCTGCCGG GAGCCTCAGC TTCCTGAAGG ACGACACAGA CGTCTATGTG 
GGTGACCGAG CTAGCGACAG CACGCGGGCC CTGCGAGGGT GTCTAAGCAC GATAGCAATC 
AGCGGCCTCT ATCTCTCTTA CTTTGAAAAC GCCCGTGGCT TGATGCACAG ACCTCAGGAG 
GAGCGGTTTC TCAAAGTCTC CGGGCCTCCA GTGGAGACGG GCTGCCTGCA GCTCGACGCC 
TGCCGCTCCG GGCCCTGCCT GCACGGAGGA CGCTGTGAGG ACGCCTACAG CTCCCACCGC 
TGCACCTGTC CCGCGGGGCG GTCGGGGCCG CACTGCGAGC TCCACGTCGA TGAATGCCTT 
TCAAGCCCCT GTATCCACGG CAACTGCTCC GCCCCCGGCG GGGCAGCCTA TCACTGCAGG 
TGTGAGCCTG GCTTCACCGG TGTGAACTGC GAGTCGGAGG TGGACCACTG CCAGCGTCAC 
CAGTGTGCAA ACGGGGCCAC CTGCGTGAGT GACGCCAACG GCTACTCTTG CCGCTGTCCC 
GGAAATTTCA CGGGAAGGTT CTGCAGATAC CTCCGATTAC CCTCCACCGT CTGTGGGAAT 
GAGAAGACCA ACCTCACTTG CTACAACGGT GGCAACTGCA CGGAGTTCCA GGGAGAAATG 
AAATGCGCCT GCTGGCCAGG TTTTACGGGG GAACGGTGTG ACAAGGACAT CGACGAGTGC 
GCGTCCGACC CCTGCCTGCA CGGCGGCCGG TGTCACGACC TGCTGAACGG GTTCCGGTGC 
GTGTGCGCGC TGGCCTTCGC AGGGCCGCGC TGCGAGCTGG ACTTGGCAGA TGACTTGCTG 
TCGGACATCT TCACCGCCAT CGGCTCAGTG ACCTTAGCCT TGTTGCTGAT CCTCTTTCTG 
GCAGTGGTGG TTTCTGTTCT CGCCTCCAAC AGAAGGGCAA CCCAGGGCTC CTACAGCCCG 
AGCCGTCAGG AGAAGGACGG CTCTCGTGTG GAGATGTGGA GCGTGATGGG TGCCCCTGCA 
GCGGAGAGGC TGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRB1 ( XM_006064882.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006064944.2 Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X3, mRNA.

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CloneID	OBu65831
Clone ID Related Accession (Same CDS sequence)	XM_006064882.2
Accession Version	XM_006064882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein crumbs homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006064882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACA CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGTTGGCA GATGACTTGC 
TGTCGGACAT CTTCACCGCC ATCGGCTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006064944.2
CDS	465..4397
Translation	MALRTVNHRLLLCLSLSLLISIKSSFCNKNDTKCLSDSCQNNSTCKDFSKDKSCRCSDTAVHGDKDCREEEDPCLSSPCPGHASCVRVPGERRSRCRCPPGHSGAGCDTAAGPCGPHSCLHGGVCHQDSGPPVCVCPAGYAGRFCELDLDECASSPCLHGAVCQDRGDGYSCFCVPGYQGRHCDVEVDECVSEPCRNAATCLNEIGRYTCLCPRGFSGVNCELEVDECWSQPCLNGATCQDAAGAYFCDCAPGFLGDRCELNMDECASGPCLHGGLCVDGANRYSCTCAGSGFTGTRCETLLPPCWSEPCHNNATCEDSADRYTCRCRPGYTGAQCETDLDGCWSGPCQAGGRCVELSSETGRERLARLLSASRRPEASRYVCICPPGLTGVHCEEDVDECSSSPCRNGGTCENSPEGYTCHCPFDPHSGVFFGGRDCSDTLLGCADHPCLNNGTCVPHVHDGQHGFDCLCPPGYTGSRCGTVTTLSFLGSGFLRVSSGSALAQDSGCTVALSFQTIQPAALLLFRGDRDAFVMLEVLAGFVHLTIQVGDRPKVVLLIPHDTSDGGWHTVEATFAEAVTLALRDDSCMGTCVARAPSPFESDGSSACTLQNSFLGGLPEGRAHSGDALLNVYRIPSAPSLVGCLQDVHLDSNAITTENLSSDQSLNVRAGCARKDWCESQPCRDRGRCLDLWLGHQCECYRPYRGRGCHGEYVAGRFGQDDSPGYAVFHLNKDYEEDLTLSMFVRTRRPAGLLLALGNGTYQYLRVWLEHGRLAMLTPGSPKLLVTFVLSDGNVRLVSLKIKPNKIELYQSSQNLGFLSAPTWKLQRGDVLYVGGLPDRRETELSGGFFKGCIQDIRLNNENLEFFPNSTSNAAHSAVLVNVTEGCPGDNLCEPNPCHHGGVCYPLWDDFSCSCPANTAGKACQELRWCELGPCPPGAQCLQLPRGFECIANAVFNGQSHEIIFRSNGNITRELTNITFGFRTRDAHVVILHAEKEPEFLRIHIRDSRLLFQLRSGNSFYTLSLTSLQPVSDGVWYQVTISMTDPGAQASRWQMEVDGQTPPVTSAVAAGSLSFLKDDTDVYVGDRASDSTRALRGCLSTIAISGLYLSYFENARGLMHRPQEERFLKVSGPPVETGCLQLDACRSGPCLHGGRCEDAYSSHRCTCPAGRSGPHCELHVDECLSSPCIHGNCSAPGGAAYHCRCEPGFTGVNCESEVDHCQRHQCANGATCVSDANGYSCRCPGNFTGRFCRYLRLPSTVCGNEKTNLTCYNGGNCTEFQGEMKCACWPGFTGERWQMTCCRTSSPPSAQ

Target ORF information:

RefSeq Version	XM_006064882.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis crumbs 1, cell polarity complex component (CRB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006064882.2

ORF Insert Sequence:

ATGGCGCTAC GGACAGTTAA CCACCGTCTC CTCCTCTGCC TCAGTCTCTC ACTGCTCATC 
TCCATTAAAA GTTCCTTTTG CAATAAAAAT GACACCAAGT GCCTCTCCGA TTCTTGCCAA 
AACAATTCTA CGTGCAAGGA TTTCTCAAAA GACAAGAGTT GCCGTTGTTC AGACACAGCC 
GTTCACGGGG ACAAAGACTG TAGGGAGGAG GAGGACCCCT GCCTCTCCAG CCCCTGTCCT 
GGACACGCCT CGTGCGTCCG CGTCCCGGGG GAGAGGCGCT CTCGGTGCAG ATGTCCTCCC 
GGGCACAGCG GGGCCGGCTG CGACACCGCC GCTGGCCCCT GCGGGCCCCA CTCCTGCCTG 
CACGGGGGCG TCTGCCACCA GGACTCTGGG CCCCCTGTGT GCGTCTGCCC CGCTGGCTAC 
GCGGGAAGGT TCTGCGAGCT GGACCTCGAT GAGTGCGCGT CTAGCCCCTG CCTGCACGGC 
GCCGTGTGCC AGGACAGAGG CGATGGCTAC TCCTGCTTCT GTGTCCCGGG CTATCAAGGC 
CGGCACTGCG ACGTGGAAGT GGACGAGTGT GTGTCGGAGC CCTGCCGGAA CGCGGCCACG 
TGCCTCAACG AGATCGGACG GTACACCTGC CTCTGCCCGC GCGGGTTCTC CGGTGTAAAC 
TGTGAACTGG AAGTTGATGA GTGTTGGTCC CAGCCCTGCC TCAATGGTGC CACTTGCCAG 
GACGCTGCGG GGGCCTACTT CTGTGACTGT GCCCCAGGAT TCCTGGGCGA TCGCTGTGAG 
CTCAACATGG ACGAGTGTGC CAGTGGGCCA TGTCTCCATG GCGGGCTGTG CGTGGATGGA 
GCCAACAGGT ACAGCTGTAC CTGCGCAGGT AGTGGGTTCA CGGGGACACG CTGTGAGACC 
CTGCTGCCCC CGTGTTGGTC AGAGCCCTGC CACAACAACG CCACCTGCGA GGACAGTGCT 
GACCGCTACA CCTGCCGCTG CCGGCCCGGA TACACGGGCG CGCAGTGTGA GACCGACCTC 
GATGGATGCT GGAGCGGCCC CTGCCAGGCC GGCGGGCGGT GCGTGGAGCT GTCCTCGGAG 
ACGGGGCGTG AGCGCCTGGC CCGGCTGCTG TCTGCATCCC GCCGCCCCGA AGCCTCCCGT 
TACGTCTGCA TCTGTCCACC CGGACTCACA GGAGTCCACT GTGAAGAGGA CGTGGACGAG 
TGCTCGTCCA GCCCTTGCCG CAATGGGGGC ACTTGTGAGA ACTCGCCTGA GGGTTACACT 
TGCCACTGCC CATTTGATCC CCATTCTGGA GTCTTCTTTG GAGGCAGGGA CTGCTCTGAT 
ACTCTCCTGG GCTGTGCCGA TCATCCGTGT CTAAACAACG GGACGTGCGT CCCTCACGTC 
CACGACGGCC AGCACGGATT CGACTGCCTG TGCCCCCCTG GCTACACCGG GTCACGGTGT 
GGAACTGTCA CCACGCTGTC CTTTTTGGGC AGTGGCTTCC TGCGGGTCTC AAGTGGCTCA 
GCTCTGGCCC AGGACTCAGG CTGTACCGTA GCCCTCAGTT TCCAGACCAT CCAGCCAGCG 
GCCCTGCTGC TCTTCCGGGG TGACCGGGAC GCGTTTGTGA TGCTGGAGGT GCTGGCTGGC 
TTTGTCCACT TGACCATTCA AGTTGGCGAT CGGCCGAAGG TGGTCCTGCT CATCCCCCAC 
GACACCAGCG ACGGAGGGTG GCACACGGTG GAGGCGACGT TTGCAGAGGC TGTGACCCTC 
GCCCTACGCG ACGACTCTTG CATGGGGACA TGCGTTGCTA GGGCTCCTTC TCCATTTGAA 
AGTGATGGGT CATCAGCGTG TACTCTCCAG AACTCCTTTT TGGGGGGTTT GCCAGAAGGA 
AGAGCCCACA GTGGTGATGC TCTGCTTAAC GTCTATAGGA TACCATCCGC ACCTTCGCTC 
GTAGGCTGCC TCCAAGACGT GCACCTGGAC TCGAATGCCA TCACCACAGA GAACCTCTCA 
TCCGACCAGT CTCTGAACGT CAGGGCAGGC TGCGCCCGAA AAGACTGGTG TGAGAGCCAG 
CCGTGTCGCG ACAGAGGACG CTGCCTCGAC CTGTGGCTCG GCCACCAGTG TGAGTGCTAC 
AGGCCCTACC GCGGCCGCGG CTGTCACGGA GAGTATGTGG CAGGCAGATT TGGCCAAGAT 
GACTCTCCTG GATATGCTGT TTTTCATCTC AACAAGGATT ATGAAGAGGA CCTCACGCTC 
TCCATGTTTG TTCGAACACG CCGTCCGGCC GGCTTGCTCT TAGCTCTGGG AAACGGCACT 
TACCAGTACC TCCGTGTCTG GCTAGAGCAT GGCAGGCTAG CGATGCTGAC TCCAGGTTCT 
CCCAAATTAT TAGTAACATT TGTTCTCAGC GACGGAAATG TCCGCTTAGT ATCTTTGAAA 
ATCAAGCCAA ATAAAATTGA ACTGTATCAG TCGTCACAAA ATCTAGGATT TCTTTCTGCT 
CCTACGTGGA AACTCCAAAG AGGAGATGTC CTCTATGTTG GTGGACTGCC TGACAGGCGA 
GAGACTGAGC TTAGTGGTGG ATTCTTCAAA GGCTGTATCC AAGATATAAG GCTAAACAAC 
GAAAACCTGG AATTCTTCCC AAATTCAACG AGCAACGCAG CCCACAGTGC CGTTCTTGTC 
AATGTGACCG AAGGCTGCCC CGGAGACAAC CTGTGTGAGC CCAACCCCTG TCACCACGGA 
GGCGTCTGCT ACCCGCTGTG GGACGACTTC TCCTGCTCCT GCCCTGCGAA CACAGCGGGG 
AAGGCCTGCC AGGAGCTGCG CTGGTGTGAA CTCGGCCCGT GTCCTCCCGG CGCCCAGTGC 
CTGCAACTGC CTCGAGGCTT CGAATGTATC GCAAATGCTG TTTTTAATGG ACAAAGCCAT 
GAGATAATAT TCAGGAGCAA CGGGAACATC ACCAGAGAGC TCACCAACAT TACCTTCGGT 
TTCAGAACGA GGGATGCCCA CGTGGTCATA CTGCACGCAG AGAAGGAGCC TGAATTCCTT 
CGTATTCACA TTAGGGATTC CCGGTTGTTA TTTCAACTGC GAAGTGGCAA CAGCTTTTAC 
ACGCTGAGTC TAACGAGTCT GCAGCCCGTC AGCGACGGCG TGTGGTACCA GGTGACCATC 
TCCATGACAG ACCCGGGGGC CCAGGCTTCC AGGTGGCAGA TGGAGGTGGA CGGGCAAACG 
CCTCCTGTGA CCAGTGCAGT GGCTGCCGGG AGCCTCAGCT TCCTGAAGGA CGACACAGAC 
GTCTATGTGG GTGACCGAGC TAGCGACAGC ACGCGGGCCC TGCGAGGGTG TCTAAGCACG 
ATAGCAATCA GCGGCCTCTA TCTCTCTTAC TTTGAAAACG CCCGTGGCTT GATGCACAGA 
CCTCAGGAGG AGCGGTTTCT CAAAGTCTCC GGGCCTCCAG TGGAGACGGG CTGCCTGCAG 
CTCGACGCCT GCCGCTCCGG GCCCTGCCTG CACGGAGGAC GCTGTGAGGA CGCCTACAGC 
TCCCACCGCT GCACCTGTCC CGCGGGGCGG TCGGGGCCGC ACTGCGAGCT CCACGTCGAT 
GAATGCCTTT CAAGCCCCTG TATCCACGGC AACTGCTCCG CCCCCGGCGG GGCAGCCTAT 
CACTGCAGGT GTGAGCCTGG CTTCACCGGT GTGAACTGCG AGTCGGAGGT GGACCACTGC 
CAGCGTCACC AGTGTGCAAA CGGGGCCACC TGCGTGAGTG ACGCCAACGG CTACTCTTGC 
CGCTGTCCCG GAAATTTCAC GGGAAGGTTC TGCAGATACC TCCGATTACC CTCCACCGTC 
TGTGGGAATG AGAAGACCAA CCTCACTTGC TACAACGGTG GCAACTGCAC GGAGTTCCAG 
GGAGAAATGA AATGCGCCTG CTGGCCAGGT TTTACGGGGG AACGTTGGCA GATGACTTGC 
TGTCGGACAT CTTCACCGCC ATCGGCTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CRB1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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