LOC102392316 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC102392316
Entrez Gene ID	102392316
Full Name	cilia and flagella associated protein 221
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006057171.1	XP_006057233.1	primary ciliary dyskinesia protein 1-like isoform X2
XM_006057170.1	XP_006057232.1	primary ciliary dyskinesia protein 1-like isoform X1
XM_006057170.2	XP_006057232.2	LOW QUALITY PROTEIN: cilia- and flagella-associated protein 221
XM_006057172.1	XP_006057234.1	primary ciliary dyskinesia protein 1-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC102392316 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC102392316 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu14175	XM_006057170.1 Latest version!	Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu14176	XM_006057171.1 Latest version!	Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu14177	XM_006057172.1 Latest version!	Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBu55673	XM_006057170.2 Latest version!	Bubalus bubalis cilia and flagella associated protein 221 (CFAP221), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC102392316 ( XM_006057170.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006057232.1 Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu14175
Clone ID Related Accession (Same CDS sequence)	XM_006057170.1
Accession Version	XM_006057170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	primary ciliary dyskinesia protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784797.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC GCAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTCGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGATGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCATGGTCAT CACCCTGCCC 
AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC 
GAGGCCTTGG CCCAAGCTCC AGATTATGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA 
TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC 
TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCCTCACC 
CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC 
CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT 
GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG 
GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AGCAGAGTAT GGACATTATG 
CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG AATACAGGGA CACCAAGAAG 
GAGAAAGAGG TGAAAGACCA AGCACAGCCA ACGGAGAAGG CAGGAGAGAA GGTGCTCGAG 
GAGCTGAAGA ACCTGCGGAG CAAAGCGCTC AGCTCCTACC TGATCCTAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006057232.1
CDS	105..2618
Translation	MAAVKTPSREVKNAEEPFQNVSPLLLRNLVEEPKRPKEVPNHLLESKVYANLLNNKVIQAKPGILHFGGYQVEKQHQQILHLVNICDEDIHVHVLPPQTKYFQIKYVKKEHRLVPGLSLRITITFSPDEWRYYYDCIRVHCKGDETLLIPIHAYPVVNTLDFPSFINLSNVLLGESKSYVIPLWCSCPVDFEFHVTVVHPHQAFTVEPTSGIIPANGKMNVTVKFTPFQYGMAQIKVQLWVSQFNSQPYECVFTGTCYPNVALKLEEFERVNTLSKKVVVPPEKAMTRVPFPQLPAKAKPPKIKEIEYHNLRFPVDLLNPFAVATVLNQEPGKLKIKELREVLDQGTEISKTRQMKEALFEQKVRQDTCEEIENHLKWQVHLGKDHMSFKFKKDIYEERQRVNNKYKLDRGDPVLYEEFQRCKTEVHPKRILHDLKEKIQEFHPNFDPLVNNTWVNRFRAQRRFQQAARKVLIHRRLLTMLNAVRNIQKDIIVKRFTQERQSMAQDKSFKYHLGRVSKDASRFSVSPQEVLPFAFPSYSPPQDSNELAPGGLGPVPIKSSEIQVKQPHSFFNLQVPQLYKIKRYQPVSVQESSASYRPQKLARALRQGAEDEATMVITLPKQDSSPQLPGETSVLSIKLPEALAQAPDYDPLYIFNPSPGLFAVKHPLTYVETLIDYHLCSHPKYKFTKECHCGSSIPLTQKQFLHHTDIIPGVMTWKRFQSLVFLSLPETSKTEVLPSCDSFNLVMLPVDVPAVLDALPEEDRIETVERELCEQSMDIMLTPEMIEAEFPMLEYRDTKKEKEVKDQAQPTEKAGEKVLEELKNLRSKALSSYLILE

Target ORF information:

RefSeq Version	XM_006057170.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006057170.1

ORF Insert Sequence:

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC GCAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTCGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGATGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCATGGTCAT CACCCTGCCC 
AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC 
GAGGCCTTGG CCCAAGCTCC AGATTATGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA 
TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC 
TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCCTCACC 
CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC 
CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT 
GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG 
GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AGCAGAGTAT GGACATTATG 
CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG AATACAGGGA CACCAAGAAG 
GAGAAAGAGG TGAAAGACCA AGCACAGCCA ACGGAGAAGG CAGGAGAGAA GGTGCTCGAG 
GAGCTGAAGA ACCTGCGGAG CAAAGCGCTC AGCTCCTACC TGATCCTAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102392316 ( XM_006057171.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006057233.1 Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu14176
Clone ID Related Accession (Same CDS sequence)	XM_006057171.1
Accession Version	XM_006057171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	primary ciliary dyskinesia protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784797.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC GCAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTCGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGATGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCATGGTCAT CACCCTGCCC 
AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC 
GAGGCCTTGG CCCAAGCTCC AGATTATGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA 
TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC 
TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCCTCACC 
CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC 
CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT 
GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG 
GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AGCAGAGTAT GGACATTATG 
CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG AATACAGGGA CACCAAGAAG 
GAGAACTTCA GCTGGAAGCA GACAGAGTGT AAAGCTTGGA CAGACAGGGA CAGAAATAAA 
AAACACATCA GTGATACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006057233.1
CDS	105..2585
Translation	MAAVKTPSREVKNAEEPFQNVSPLLLRNLVEEPKRPKEVPNHLLESKVYANLLNNKVIQAKPGILHFGGYQVEKQHQQILHLVNICDEDIHVHVLPPQTKYFQIKYVKKEHRLVPGLSLRITITFSPDEWRYYYDCIRVHCKGDETLLIPIHAYPVVNTLDFPSFINLSNVLLGESKSYVIPLWCSCPVDFEFHVTVVHPHQAFTVEPTSGIIPANGKMNVTVKFTPFQYGMAQIKVQLWVSQFNSQPYECVFTGTCYPNVALKLEEFERVNTLSKKVVVPPEKAMTRVPFPQLPAKAKPPKIKEIEYHNLRFPVDLLNPFAVATVLNQEPGKLKIKELREVLDQGTEISKTRQMKEALFEQKVRQDTCEEIENHLKWQVHLGKDHMSFKFKKDIYEERQRVNNKYKLDRGDPVLYEEFQRCKTEVHPKRILHDLKEKIQEFHPNFDPLVNNTWVNRFRAQRRFQQAARKVLIHRRLLTMLNAVRNIQKDIIVKRFTQERQSMAQDKSFKYHLGRVSKDASRFSVSPQEVLPFAFPSYSPPQDSNELAPGGLGPVPIKSSEIQVKQPHSFFNLQVPQLYKIKRYQPVSVQESSASYRPQKLARALRQGAEDEATMVITLPKQDSSPQLPGETSVLSIKLPEALAQAPDYDPLYIFNPSPGLFAVKHPLTYVETLIDYHLCSHPKYKFTKECHCGSSIPLTQKQFLHHTDIIPGVMTWKRFQSLVFLSLPETSKTEVLPSCDSFNLVMLPVDVPAVLDALPEEDRIETVERELCEQSMDIMLTPEMIEAEFPMLEYRDTKKENFSWKQTECKAWTDRDRNKKHISDT

Target ORF information:

RefSeq Version	XM_006057171.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006057171.1

ORF Insert Sequence:

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC GCAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTCGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGATGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCATGGTCAT CACCCTGCCC 
AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC 
GAGGCCTTGG CCCAAGCTCC AGATTATGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA 
TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC 
TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCCTCACC 
CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC 
CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT 
GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG 
GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AGCAGAGTAT GGACATTATG 
CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG AATACAGGGA CACCAAGAAG 
GAGAACTTCA GCTGGAAGCA GACAGAGTGT AAAGCTTGGA CAGACAGGGA CAGAAATAAA 
AAACACATCA GTGATACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102392316 ( XM_006057172.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006057234.1 Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X3, mRNA.

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CloneID	OBu14177
Clone ID Related Accession (Same CDS sequence)	XM_006057172.1
Accession Version	XM_006057172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2379bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	primary ciliary dyskinesia protein 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784797.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC GCAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTCGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGATGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG AATCCCAGCC CGGGATTGTT TGCTGTGAAG 
CACCCTCTGA CTTACGTGGA AACCTTGATC GATTACCACC TGTGCTCCCA CCCCAAGTAC 
AAGTTCACCA AGGAGTGCCA CTGCGGCTCC AGCATCCCCC TCACCCAGAA GCAATTTCTC 
CATCACACGG ACATAATACC TGGAGTGATG ACCTGGAAGA GGTTCCAGAG CCTGGTGTTC 
TTATCCCTGC CCGAAACTTC TAAGACAGAG GTGTTGCCGA GCTGTGATTC CTTCAACTTA 
GTCATGCTGC CTGTCGACGT CCCTGCCGTT CTTGACGCCT TGCCGGAAGA AGACAGGATA 
GAGACAGTTG AACGTGAGCT CTGTGAGCAG AGTATGGACA TTATGCTGAC TCCAGAAATG 
ATCGAAGCGG AATTCCCAAT GCTGGAATAC AGGGACACCA AGAAGGAGAA AGAGGTGAAA 
GACCAAGCAC AGCCAACGGA GAAGGCAGGA GAGAAGGTGC TCGAGGAGCT GAAGAACCTG 
CGGAGCAAAG CGCTCAGCTC CTACCTGATC CTAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006057234.1
CDS	105..2483
Translation	MAAVKTPSREVKNAEEPFQNVSPLLLRNLVEEPKRPKEVPNHLLESKVYANLLNNKVIQAKPGILHFGGYQVEKQHQQILHLVNICDEDIHVHVLPPQTKYFQIKYVKKEHRLVPGLSLRITITFSPDEWRYYYDCIRVHCKGDETLLIPIHAYPVVNTLDFPSFINLSNVLLGESKSYVIPLWCSCPVDFEFHVTVVHPHQAFTVEPTSGIIPANGKMNVTVKFTPFQYGMAQIKVQLWVSQFNSQPYECVFTGTCYPNVALKLEEFERVNTLSKKVVVPPEKAMTRVPFPQLPAKAKPPKIKEIEYHNLRFPVDLLNPFAVATVLNQEPGKLKIKELREVLDQGTEISKTRQMKEALFEQKVRQDTCEEIENHLKWQVHLGKDHMSFKFKKDIYEERQRVNNKYKLDRGDPVLYEEFQRCKTEVHPKRILHDLKEKIQEFHPNFDPLVNNTWVNRFRAQRRFQQAARKVLIHRRLLTMLNAVRNIQKDIIVKRFTQERQSMAQDKSFKYHLGRVSKDASRFSVSPQEVLPFAFPSYSPPQDSNELAPGGLGPVPIKSSEIQVKQPHSFFNLQVPQLYKIKRYQPVSVQESSASYRPQKLARALRQGAENPSPGLFAVKHPLTYVETLIDYHLCSHPKYKFTKECHCGSSIPLTQKQFLHHTDIIPGVMTWKRFQSLVFLSLPETSKTEVLPSCDSFNLVMLPVDVPAVLDALPEEDRIETVERELCEQSMDIMLTPEMIEAEFPMLEYRDTKKEKEVKDQAQPTEKAGEKVLEELKNLRSKALSSYLILE

Target ORF information:

RefSeq Version	XM_006057172.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis primary ciliary dyskinesia protein 1-like (LOC102392316), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006057172.1

ORF Insert Sequence:

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC GCAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTCGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTTCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGATGCAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG AATCCCAGCC CGGGATTGTT TGCTGTGAAG 
CACCCTCTGA CTTACGTGGA AACCTTGATC GATTACCACC TGTGCTCCCA CCCCAAGTAC 
AAGTTCACCA AGGAGTGCCA CTGCGGCTCC AGCATCCCCC TCACCCAGAA GCAATTTCTC 
CATCACACGG ACATAATACC TGGAGTGATG ACCTGGAAGA GGTTCCAGAG CCTGGTGTTC 
TTATCCCTGC CCGAAACTTC TAAGACAGAG GTGTTGCCGA GCTGTGATTC CTTCAACTTA 
GTCATGCTGC CTGTCGACGT CCCTGCCGTT CTTGACGCCT TGCCGGAAGA AGACAGGATA 
GAGACAGTTG AACGTGAGCT CTGTGAGCAG AGTATGGACA TTATGCTGAC TCCAGAAATG 
ATCGAAGCGG AATTCCCAAT GCTGGAATAC AGGGACACCA AGAAGGAGAA AGAGGTGAAA 
GACCAAGCAC AGCCAACGGA GAAGGCAGGA GAGAAGGTGC TCGAGGAGCT GAAGAACCTG 
CGGAGCAAAG CGCTCAGCTC CTACCTGATC CTAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC102392316 ( XM_006057170.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006057232.2 Bubalus bubalis cilia and flagella associated protein 221 (CFAP221), mRNA.

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CloneID	OBu55673
Clone ID Related Accession (Same CDS sequence)	XM_006057170.2
Accession Version	XM_006057170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: cilia- and flagella-associated protein 221
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006057170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC ACAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTCCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGACTGAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCATGGTCAT CACCCTGCCC 
AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC 
GAGGCCTTGG CCCAAGCTCC AGATTATGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA 
TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC 
TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCCTCACC 
CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC 
CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT 
GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG 
GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AGCAGAGTAT GGACATTATG 
CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG ACTACAGGGA CACCAAGAAG 
GAGAAAGAGG TGAAAGACCA AGCACAGCCA ACGGAGAAGG CAGGAGAGAA GGTGCTCGAG 
GAGCTGAAGA ACCTGCGGAG CAAAGCGCTC AGCTCCTACC TGATCCTAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006057232.2
CDS	157..2670
Translation	MAAVKTPSREVKNAEEPFQNVSPLLLRNLVEEPKRPKEVPNHLLESKVYANLLNNKVIQAKPGILHFGGYQVEKQHQQILHLVNICDEDIHVHVLPPQTKYFQIKYVKKEHRLVPGLSLRITITFSPDEWRYYYDCIRVHCKGDETLLIPIHAYPVVNTLDFPSFINLSNVLLGESKSYVIPLWCSCPVDFEFHVTVVHPHQAFTVEPTSGIIPANGKMNVTVKFTPFQYGMAQIKVQLWVSQFNSQPYECVFTGTCYPNVALKLEEFERVNTLSKKVVVPPEKAMTRVPFPQLPAKAKPPKIKEIEYHNLRFPVDLLNPFAVATVLNQEPGKLKIKELREVLDQGTEISKTRQMKEALFEQKVRQDTCEEIENHLKWQVHLGKDHMSFKFKKDIYEERQRVNNKYKLDRGDPVLYEEFQRLKTEVHPKRILHDLKEKIQEFHPNFDPLVNNTWVNRFRAQRRFQQAARKVLIHRRLLTMLNAVRNIQKDIIVKRFTQERQSMAQDKSFKYHLGRVSKDASRFSVSPQEVLPFAFPSYSPPQDSNELAPGGLGPVPIKSSEIQVKQPHSFFNLQVPQLYKIKRYQPVSVQESSASYRPQKLARALRQGAEDEATMVITLPKQDSSPQLPGETSVLSIKLPEALAQAPDYDPLYIFNPSPGLFAVKHPLTYVETLIDYHLCSHPKYKFTKECHCGSSIPLTQKQFLHHTDIIPGVMTWKRFQSLVFLSLPETSKTEVLPSCDSFNLVMLPVDVPAVLDALPEEDRIETVERELCEQSMDIMLTPEMIEAEFPMLDYRDTKKEKEVKDQAQPTEKAGEKVLEELKNLRSKALSSYLILE

Target ORF information:

RefSeq Version	XM_006057170.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis cilia and flagella associated protein 221 (CFAP221), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006057170.2

ORF Insert Sequence:

ATGGCAGCGG TGAAAACCCC CAGCAGAGAA GTAAAGAATG CTGAAGAACC ATTTCAAAAT 
GTGTCACCCC TCCTTTTAAG GAACCTAGTG GAGGAACCCA AGAGACCAAA AGAAGTACCC 
AATCATCTCC TAGAGTCAAA GGTTTATGCG AATCTTCTGA ATAATAAGGT CATACAGGCA 
AAACCTGGCA TATTACATTT TGGAGGCTAT CAAGTAGAAA AACAACACCA GCAGATATTG 
CATCTGGTCA ATATTTGTGA TGAAGACATA CATGTTCATG TTTTACCCCC ACAAACCAAA 
TACTTTCAGA TCAAGTATGT GAAAAAGGAG CACCGCCTTG TCCCTGGCTT GTCTCTCAGG 
ATCACCATTA CATTTTCTCC AGATGAGTGG CGATACTATT ATGATTGCAT CCGTGTTCAC 
TGTAAGGGAG ATGAGACTTT GCTTATTCCC ATTCATGCCT ATCCTGTCGT GAACACACTG 
GACTTTCCCT CATTTATAAA TCTATCAAAT GTTCTACTTG GTGAAAGCAA AAGCTACGTG 
ATTCCTTTGT GGTGCAGTTG CCCTGTGGAT TTTGAGTTTC ATGTCACTGT GGTTCATCCT 
CATCAGGCCT TTACTGTGGA GCCAACATCA GGAATAATCC CGGCGAATGG GAAGATGAAT 
GTGACAGTGA AGTTTACCCC GTTTCAGTAT GGGATGGCAC AGATAAAAGT GCAGCTGTGG 
GTTTCACAGT TCAACTCGCA ACCGTACGAG TGTGTCTTCA CCGGGACATG CTACCCCAAT 
GTGGCTTTAA AGTTGGAAGA ATTTGAAAGA GTAAATACTC TTTCTAAGAA AGTAGTTGTT 
CCTCCAGAAA AGGCAATGAC ACGTGTACCT TTCCCCCAAT TGCCAGCAAA AGCAAAGCCT 
CCAAAGATTA AGGAAATCGA ATATCACAAC TTGAGATTTC CAGTAGATTT ATTGAATCCA 
TTTGCTGTGG CAACTGTTTT AAACCAAGAA CCAGGAAAAT TAAAGATTAA AGAATTGAGA 
GAAGTTTTGG ACCAAGGCAC TGAGATTTCA AAAACAAGAC AAATGAAAGA AGCGCTCTTT 
GAACAGAAAG TCAGACAGGA CACTTGTGAA GAGATTGAAA ATCACCTGAA GTGGCAGGTG 
CACCTCGGTA AGGATCATAT GTCCTTTAAA TTTAAAAAAG ACATTTATGA GGAGCGGCAA 
AGAGTAAACA ACAAATACAA GCTAGATAGA GGAGATCCTG TTTTGTATGA GGAATTCCAG 
CGACTGAAGA CAGAAGTTCA CCCCAAACGG ATTTTACACG ACCTGAAAGA AAAAATCCAA 
GAATTTCATC CTAATTTTGA TCCACTCGTT AATAATACTT GGGTCAACAG ATTCCGAGCA 
CAGAGACGGT TTCAACAAGC AGCTCGCAAG GTCCTGATTC ATCGCCGCTT GCTTACTATG 
CTGAATGCCG TCCGTAACAT ACAGAAAGAC ATCATCGTGA AAAGGTTCAC TCAGGAAAGA 
CAGTCCATGG CACAAGACAA GAGCTTCAAA TACCACCTGG GGCGAGTCAG TAAGGACGCC 
AGCCGGTTCT CTGTGTCTCC CCAGGAAGTA CTGCCCTTTG CCTTTCCATC CTACAGCCCT 
CCCCAGGACT CCAACGAACT GGCTCCTGGT GGCCTTGGAC CTGTCCCAAT TAAATCTTCA 
GAAATTCAAG TCAAGCAGCC CCATTCCTTC TTCAATCTGC AGGTTCCTCA GCTATACAAG 
ATCAAGAGGT ACCAGCCGGT CTCTGTGCAG GAGTCTTCAG CCAGCTACAG ACCTCAGAAG 
CTTGCTCGGG CCCTGAGGCA AGGAGCTGAG GACGAAGCCA CCATGGTCAT CACCCTGCCC 
AAGCAGGATT CCTCGCCCCA GCTGCCCGGT GAGACCTCCG TCCTGAGCAT AAAACTCCCC 
GAGGCCTTGG CCCAAGCTCC AGATTATGAC CCGCTTTATA TTTTTAATCC CAGCCCGGGA 
TTGTTTGCTG TGAAGCACCC TCTGACTTAC GTGGAAACCT TGATCGATTA CCACCTGTGC 
TCCCACCCCA AGTACAAGTT CACCAAGGAG TGCCACTGCG GCTCCAGCAT CCCCCTCACC 
CAGAAGCAAT TTCTCCATCA CACGGACATA ATACCTGGAG TGATGACCTG GAAGAGGTTC 
CAGAGCCTGG TGTTCTTATC CCTGCCCGAA ACTTCTAAGA CAGAGGTGTT GCCGAGCTGT 
GATTCCTTCA ACTTAGTCAT GCTGCCTGTC GACGTCCCTG CCGTTCTTGA CGCCTTGCCG 
GAAGAAGACA GGATAGAGAC AGTTGAACGT GAGCTCTGTG AGCAGAGTAT GGACATTATG 
CTGACTCCAG AAATGATCGA AGCGGAATTC CCAATGCTGG ACTACAGGGA CACCAAGAAG 
GAGAAAGAGG TGAAAGACCA AGCACAGCCA ACGGAGAAGG CAGGAGAGAA GGTGCTCGAG 
GAGCTGAAGA ACCTGCGGAG CAAAGCGCTC AGCTCCTACC TGATCCTAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC102392316 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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