ATAD2B gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_006050619.1	XP_006050681.1	ATPase family AAA domain-containing protein 2B isoform X2
XM_006050620.1	XP_006050682.1	ATPase family AAA domain-containing protein 2B isoform X3
XM_006050622.1	XP_006050684.1	ATPase family AAA domain-containing protein 2B isoform X5
XM_025261499.1	XP_025117284.1	ATPase family AAA domain-containing protein 2B isoform X2
XM_025261500.1	XP_025117285.1	ATPase family AAA domain-containing protein 2B isoform X3
XM_006050618.2	XP_006050680.1	ATPase family AAA domain-containing protein 2B isoform X4
XM_006050621.2	XP_006050683.1	ATPase family AAA domain-containing protein 2B isoform X8
XM_006050621.1	XP_006050683.1	ATPase family AAA domain-containing protein 2B isoform X4
XM_025261503.1	XP_025117288.1	ATPase family AAA domain-containing protein 2B isoform X9
XM_006050618.1	XP_006050680.1	ATPase family AAA domain-containing protein 2B isoform X1
XM_006050619.2	XP_006050681.1	ATPase family AAA domain-containing protein 2B isoform X6
XM_025261504.1	XP_025117289.1	ATPase family AAA domain-containing protein 2B isoform X10
XM_025261498.1	XP_025117283.1	ATPase family AAA domain-containing protein 2B isoform X1
XM_025261501.1	XP_025117286.1	ATPase family AAA domain-containing protein 2B isoform X5
XM_006050620.2	XP_006050682.1	ATPase family AAA domain-containing protein 2B isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu08666	XM_006050618.2 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu08667	XM_006050619.2 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu08668	XM_006050620.2 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu08669	XM_006050621.2 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu08670	XM_006050622.1 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu46438	XM_025261500.1 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu46439	XM_025261501.1 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu46440	XM_025261503.1 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu46441	XM_025261504.1 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46436	XM_025261498.1 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBu46437	XM_025261499.1 Latest version!	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OBu08669	XM_006050621.1	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu08666	XM_006050618.1	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu08667	XM_006050619.1	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBu08668	XM_006050620.1	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

ATAD2B ( XM_006050618.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050680.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu08666
Clone ID Related Accession (Same CDS sequence)	XM_006050618.2 , XM_006050618.1
Accession Version	XM_006050618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGGAG 
ATGGAAAGAA CAGTTCATAT GTTTGAGACA TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050680.1
CDS	320..4738
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050618.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050618.2

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGGAG 
ATGGAAAGAA CAGTTCATAT GTTTGAGACA TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050618.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050680.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu08666
Clone ID Related Accession (Same CDS sequence)	XM_006050618.2 , XM_006050618.1
Accession Version	XM_006050618.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784308.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGGAG 
ATGGAAAGAA CAGTTCATAT GTTTGAGACA TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050680.1
CDS	368..4786
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050618.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050618.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGGAG 
ATGGAAAGAA CAGTTCATAT GTTTGAGACA TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050619.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050681.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X2, mRNA.

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CloneID	OBu08667
Clone ID Related Accession (Same CDS sequence)	XM_006050619.1 , XM_006050619.2
Accession Version	XM_006050619.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784308.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTTTCAGATT ATCTTGAAGT AATCAAGGAA CCAATGGACT TATCAACAGT AATAACTAAA 
ATTGATAAGC ATAATTACCT GACTGCTAAG GATTTCCTAA AAGATATTGA CCTCATCTGT 
AGCAATGCTT TGGAGTACAA TCCAGATAAG GACCCAGGAG ACAAAATAAT TAGGCACAGG 
GCTTGTACTC TGAAGGATAC TGCACATGCC ATCATTGCAG CTGAGTTGGA TCCAGAATTT 
AATAAACTCT GTGAAGAAAT TAAGGAAGCA AGAATAAAAA GAGGCTTATC GGTAACAGCA 
GAACAAATAA ATCCTCATGG TACTGGAGCT CGAAAGACAG AAACTAGAGT TGAAGAGGCA 
TTTCGGCACA AACAAAGAAA TCCAATGGAT GTCTGGCACA GTTCTGCAAA TAAATGTGCA 
TTTCGAGTTC GGAGAAAATC ACGGCGGCGA TCACAGTGGG GTAAAGGAAT TATTAAGAAA 
AGGAAAGTCA ATAATTTAAA AAAAGATGAA GAGGACACCA AATTTACTGA CTATGAGAAT 
CATATGGAGG ACAGGAAATT GCTCGAAAAT GGAGAGTTTG AGGTAAGCAC TGACTGCCAT 
GAAGAAAATG GAGAAGAGAC TGGAGACTTA TCTATGACCA ATGATGAATC ATCCTGTGAC 
ATCATGGACA TGGACCAGGG GCAGAGGCTT AACAATGGAG CAAGCACAAA AGAGAACTTT 
GCATCTACTG AAGAGGAAAG TTCAAATGAG TCTCTACTCA TCAACAGCAG CAGTTCTCTA 
AATCCAGAAC AGACATCTCG GAAAGAGCCT TTCCTTAAAG AAGGCTGTCT AAATGGTGAG 
GCCTCCACAG ACAGTTTTGA AGGAATACCA GTTCTGGAGT GTCAGAATGG CAAAGCTGAA 
GTAGTTTCTT GCTGTGGCAG TGGAGATAAA TGTAGTTCTG AACAAAAGAC TCTCCTGGAG 
GACCACTCAA AAGAAAAACC AGAAACTTCA AATGAAAATC ATGGAGATGA TCCTGAGAAA 
CTAGAGGCAC TGGAATGTAG CACTAACGAG AAGTTAGAGC CTGGCCCTGA TGTAGAGGTT 
AAAGATGCAG AACTGGATAA AGAAGGTGCT TCTAAAGTAA AGAAATATCG TAAGTTAATT 
TTAGAACAGG CAAAAACAAC AAGCCTGGAA CTGGTTCCAG AAGAGCCATC TGAGCCTGTG 
CCACCTCTTA TAGTTGATCA TGAGAGATTG AAGAAATTGC TTGATTTGTT GGTAGATAAA 
AGCAACAATC TGGCAGTTGA TCAACTTGAG AGATTATATT CTCTTCTTAG TCAGTGCATC 
TACCGTCACC GTAAAGATTA TGACAAATCA CAACTTGTAG AGGAGATGGA AAGAACAGTT 
CATATGTTTG AGACATTCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050681.1
CDS	368..4771
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050619.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050619.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTTTCAGATT ATCTTGAAGT AATCAAGGAA CCAATGGACT TATCAACAGT AATAACTAAA 
ATTGATAAGC ATAATTACCT GACTGCTAAG GATTTCCTAA AAGATATTGA CCTCATCTGT 
AGCAATGCTT TGGAGTACAA TCCAGATAAG GACCCAGGAG ACAAAATAAT TAGGCACAGG 
GCTTGTACTC TGAAGGATAC TGCACATGCC ATCATTGCAG CTGAGTTGGA TCCAGAATTT 
AATAAACTCT GTGAAGAAAT TAAGGAAGCA AGAATAAAAA GAGGCTTATC GGTAACAGCA 
GAACAAATAA ATCCTCATGG TACTGGAGCT CGAAAGACAG AAACTAGAGT TGAAGAGGCA 
TTTCGGCACA AACAAAGAAA TCCAATGGAT GTCTGGCACA GTTCTGCAAA TAAATGTGCA 
TTTCGAGTTC GGAGAAAATC ACGGCGGCGA TCACAGTGGG GTAAAGGAAT TATTAAGAAA 
AGGAAAGTCA ATAATTTAAA AAAAGATGAA GAGGACACCA AATTTACTGA CTATGAGAAT 
CATATGGAGG ACAGGAAATT GCTCGAAAAT GGAGAGTTTG AGGTAAGCAC TGACTGCCAT 
GAAGAAAATG GAGAAGAGAC TGGAGACTTA TCTATGACCA ATGATGAATC ATCCTGTGAC 
ATCATGGACA TGGACCAGGG GCAGAGGCTT AACAATGGAG CAAGCACAAA AGAGAACTTT 
GCATCTACTG AAGAGGAAAG TTCAAATGAG TCTCTACTCA TCAACAGCAG CAGTTCTCTA 
AATCCAGAAC AGACATCTCG GAAAGAGCCT TTCCTTAAAG AAGGCTGTCT AAATGGTGAG 
GCCTCCACAG ACAGTTTTGA AGGAATACCA GTTCTGGAGT GTCAGAATGG CAAAGCTGAA 
GTAGTTTCTT GCTGTGGCAG TGGAGATAAA TGTAGTTCTG AACAAAAGAC TCTCCTGGAG 
GACCACTCAA AAGAAAAACC AGAAACTTCA AATGAAAATC ATGGAGATGA TCCTGAGAAA 
CTAGAGGCAC TGGAATGTAG CACTAACGAG AAGTTAGAGC CTGGCCCTGA TGTAGAGGTT 
AAAGATGCAG AACTGGATAA AGAAGGTGCT TCTAAAGTAA AGAAATATCG TAAGTTAATT 
TTAGAACAGG CAAAAACAAC AAGCCTGGAA CTGGTTCCAG AAGAGCCATC TGAGCCTGTG 
CCACCTCTTA TAGTTGATCA TGAGAGATTG AAGAAATTGC TTGATTTGTT GGTAGATAAA 
AGCAACAATC TGGCAGTTGA TCAACTTGAG AGATTATATT CTCTTCTTAG TCAGTGCATC 
TACCGTCACC GTAAAGATTA TGACAAATCA CAACTTGTAG AGGAGATGGA AAGAACAGTT 
CATATGTTTG AGACATTCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050619.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050681.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X6, mRNA.

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CloneID	OBu08667
Clone ID Related Accession (Same CDS sequence)	XM_006050619.1 , XM_006050619.2
Accession Version	XM_006050619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTTTCAGATT ATCTTGAAGT AATCAAGGAA CCAATGGACT TATCAACAGT AATAACTAAA 
ATTGATAAGC ATAATTACCT GACTGCTAAG GATTTCCTAA AAGATATTGA CCTCATCTGT 
AGCAATGCTT TGGAGTACAA TCCAGATAAG GACCCAGGAG ACAAAATAAT TAGGCACAGG 
GCTTGTACTC TGAAGGATAC TGCACATGCC ATCATTGCAG CTGAGTTGGA TCCAGAATTT 
AATAAACTCT GTGAAGAAAT TAAGGAAGCA AGAATAAAAA GAGGCTTATC GGTAACAGCA 
GAACAAATAA ATCCTCATGG TACTGGAGCT CGAAAGACAG AAACTAGAGT TGAAGAGGCA 
TTTCGGCACA AACAAAGAAA TCCAATGGAT GTCTGGCACA GTTCTGCAAA TAAATGTGCA 
TTTCGAGTTC GGAGAAAATC ACGGCGGCGA TCACAGTGGG GTAAAGGAAT TATTAAGAAA 
AGGAAAGTCA ATAATTTAAA AAAAGATGAA GAGGACACCA AATTTACTGA CTATGAGAAT 
CATATGGAGG ACAGGAAATT GCTCGAAAAT GGAGAGTTTG AGGTAAGCAC TGACTGCCAT 
GAAGAAAATG GAGAAGAGAC TGGAGACTTA TCTATGACCA ATGATGAATC ATCCTGTGAC 
ATCATGGACA TGGACCAGGG GCAGAGGCTT AACAATGGAG CAAGCACAAA AGAGAACTTT 
GCATCTACTG AAGAGGAAAG TTCAAATGAG TCTCTACTCA TCAACAGCAG CAGTTCTCTA 
AATCCAGAAC AGACATCTCG GAAAGAGCCT TTCCTTAAAG AAGGCTGTCT AAATGGTGAG 
GCCTCCACAG ACAGTTTTGA AGGAATACCA GTTCTGGAGT GTCAGAATGG CAAAGCTGAA 
GTAGTTTCTT GCTGTGGCAG TGGAGATAAA TGTAGTTCTG AACAAAAGAC TCTCCTGGAG 
GACCACTCAA AAGAAAAACC AGAAACTTCA AATGAAAATC ATGGAGATGA TCCTGAGAAA 
CTAGAGGCAC TGGAATGTAG CACTAACGAG AAGTTAGAGC CTGGCCCTGA TGTAGAGGTT 
AAAGATGCAG AACTGGATAA AGAAGGTGCT TCTAAAGTAA AGAAATATCG TAAGTTAATT 
TTAGAACAGG CAAAAACAAC AAGCCTGGAA CTGGTTCCAG AAGAGCCATC TGAGCCTGTG 
CCACCTCTTA TAGTTGATCA TGAGAGATTG AAGAAATTGC TTGATTTGTT GGTAGATAAA 
AGCAACAATC TGGCAGTTGA TCAACTTGAG AGATTATATT CTCTTCTTAG TCAGTGCATC 
TACCGTCACC GTAAAGATTA TGACAAATCA CAACTTGTAG AGGAGATGGA AAGAACAGTT 
CATATGTTTG AGACATTCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050681.1
CDS	320..4723
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050619.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050619.2

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTTTCAGATT ATCTTGAAGT AATCAAGGAA CCAATGGACT TATCAACAGT AATAACTAAA 
ATTGATAAGC ATAATTACCT GACTGCTAAG GATTTCCTAA AAGATATTGA CCTCATCTGT 
AGCAATGCTT TGGAGTACAA TCCAGATAAG GACCCAGGAG ACAAAATAAT TAGGCACAGG 
GCTTGTACTC TGAAGGATAC TGCACATGCC ATCATTGCAG CTGAGTTGGA TCCAGAATTT 
AATAAACTCT GTGAAGAAAT TAAGGAAGCA AGAATAAAAA GAGGCTTATC GGTAACAGCA 
GAACAAATAA ATCCTCATGG TACTGGAGCT CGAAAGACAG AAACTAGAGT TGAAGAGGCA 
TTTCGGCACA AACAAAGAAA TCCAATGGAT GTCTGGCACA GTTCTGCAAA TAAATGTGCA 
TTTCGAGTTC GGAGAAAATC ACGGCGGCGA TCACAGTGGG GTAAAGGAAT TATTAAGAAA 
AGGAAAGTCA ATAATTTAAA AAAAGATGAA GAGGACACCA AATTTACTGA CTATGAGAAT 
CATATGGAGG ACAGGAAATT GCTCGAAAAT GGAGAGTTTG AGGTAAGCAC TGACTGCCAT 
GAAGAAAATG GAGAAGAGAC TGGAGACTTA TCTATGACCA ATGATGAATC ATCCTGTGAC 
ATCATGGACA TGGACCAGGG GCAGAGGCTT AACAATGGAG CAAGCACAAA AGAGAACTTT 
GCATCTACTG AAGAGGAAAG TTCAAATGAG TCTCTACTCA TCAACAGCAG CAGTTCTCTA 
AATCCAGAAC AGACATCTCG GAAAGAGCCT TTCCTTAAAG AAGGCTGTCT AAATGGTGAG 
GCCTCCACAG ACAGTTTTGA AGGAATACCA GTTCTGGAGT GTCAGAATGG CAAAGCTGAA 
GTAGTTTCTT GCTGTGGCAG TGGAGATAAA TGTAGTTCTG AACAAAAGAC TCTCCTGGAG 
GACCACTCAA AAGAAAAACC AGAAACTTCA AATGAAAATC ATGGAGATGA TCCTGAGAAA 
CTAGAGGCAC TGGAATGTAG CACTAACGAG AAGTTAGAGC CTGGCCCTGA TGTAGAGGTT 
AAAGATGCAG AACTGGATAA AGAAGGTGCT TCTAAAGTAA AGAAATATCG TAAGTTAATT 
TTAGAACAGG CAAAAACAAC AAGCCTGGAA CTGGTTCCAG AAGAGCCATC TGAGCCTGTG 
CCACCTCTTA TAGTTGATCA TGAGAGATTG AAGAAATTGC TTGATTTGTT GGTAGATAAA 
AGCAACAATC TGGCAGTTGA TCAACTTGAG AGATTATATT CTCTTCTTAG TCAGTGCATC 
TACCGTCACC GTAAAGATTA TGACAAATCA CAACTTGTAG AGGAGATGGA AAGAACAGTT 
CATATGTTTG AGACATTCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050620.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050682.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu08668
Clone ID Related Accession (Same CDS sequence)	XM_006050620.1 , XM_006050620.2
Accession Version	XM_006050620.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784308.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT GGATATTGAA GAGGTTTCAG ATTATCTTGA AGTAATCAAG 
GAACCAATGG ACTTATCAAC AGTAATAACT AAAATTGATA AGCATAATTA CCTGACTGCT 
AAGGATTTCC TAAAAGATAT TGACCTCATC TGTAGCAATG CTTTGGAGTA CAATCCAGAT 
AAGGACCCAG GAGACAAAAT AATTAGGCAC AGGGCTTGTA CTCTGAAGGA TACTGCACAT 
GCCATCATTG CAGCTGAGTT GGATCCAGAA TTTAATAAAC TCTGTGAAGA AATTAAGGAA 
GCAAGAATAA AAAGAGGCTT ATCGGTAACA GCAGAACAAA TAAATCCTCA TGGTACTGGA 
GCTCGAAAGA CAGAAACTAG AGTTGAAGAG GCATTTCGGC ACAAACAAAG AAATCCAATG 
GATGTCTGGC ACAGTTCTGC AAATAAATGT GCATTTCGAG TTCGGAGAAA ATCACGGCGG 
CGATCACAGT GGGGTAAAGG AATTATTAAG AAAAGGAAAG TCAATAATTT AAAAAAAGAT 
GAAGAGGACA CCAAATTTAC TGACTATGAG AATCATATGG AGGACAGGAA ATTGCTCGAA 
AATGGAGAGT TTGAGGTAAG CACTGACTGC CATGAAGAAA ATGGAGAAGA GACTGGAGAC 
TTATCTATGA CCAATGATGA ATCATCCTGT GACATCATGG ACATGGACCA GGGGCAGAGG 
CTTAACAATG GAGCAAGCAC AAAAGAGAAC TTTGCATCTA CTGAAGAGGA AAGTTCAAAT 
GAGTCTCTAC TCATCAACAG CAGCAGTTCT CTAAATCCAG AACAGACATC TCGGAAAGAG 
CCTTTCCTTA AAGAAGGCTG TCTAAATGGT GAGGCCTCCA CAGACAGTTT TGAAGGAATA 
CCAGTTCTGG AGTGTCAGAA TGGCAAAGCT GAAGTAGTTT CTTGCTGTGG CAGTGGAGAT 
AAATGTAGTT CTGAACAAAA GACTCTCCTG GAGGACCACT CAAAAGAAAA ACCAGAAACT 
TCAAATGAAA ATCATGGAGA TGATCCTGAG AAACTAGAGG CACTGGAATG TAGCACTAAC 
GAGAAGTTAG AGCCTGGCCC TGATGTAGAG GTTAAAGATG CAGAACTGGA TAAAGAAGGT 
GCTTCTAAAG TAAAGAAATA TCGTAAGTTA ATTTTAGAAC AGGCAAAAAC AACAAGCCTG 
GAACTGGTTC CAGAAGAGCC ATCTGAGCCT GTGCCACCTC TTATAGTTGA TCATGAGAGA 
TTGAAGAAAT TGCTTGATTT GTTGGTAGAT AAAAGCAACA ATCTGGCAGT TGATCAACTT 
GAGAGATTAT ATTCTCTTCT TAGTCAGTGC ATCTACCGTC ACCGTAAAGA TTATGACAAA 
TCACAACTTG TAGAGGAGAT GGAAAGAACA GTTCATATGT TTGAGACATT CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050682.1
CDS	367..4743
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050620.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050620.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT GGATATTGAA GAGGTTTCAG ATTATCTTGA AGTAATCAAG 
GAACCAATGG ACTTATCAAC AGTAATAACT AAAATTGATA AGCATAATTA CCTGACTGCT 
AAGGATTTCC TAAAAGATAT TGACCTCATC TGTAGCAATG CTTTGGAGTA CAATCCAGAT 
AAGGACCCAG GAGACAAAAT AATTAGGCAC AGGGCTTGTA CTCTGAAGGA TACTGCACAT 
GCCATCATTG CAGCTGAGTT GGATCCAGAA TTTAATAAAC TCTGTGAAGA AATTAAGGAA 
GCAAGAATAA AAAGAGGCTT ATCGGTAACA GCAGAACAAA TAAATCCTCA TGGTACTGGA 
GCTCGAAAGA CAGAAACTAG AGTTGAAGAG GCATTTCGGC ACAAACAAAG AAATCCAATG 
GATGTCTGGC ACAGTTCTGC AAATAAATGT GCATTTCGAG TTCGGAGAAA ATCACGGCGG 
CGATCACAGT GGGGTAAAGG AATTATTAAG AAAAGGAAAG TCAATAATTT AAAAAAAGAT 
GAAGAGGACA CCAAATTTAC TGACTATGAG AATCATATGG AGGACAGGAA ATTGCTCGAA 
AATGGAGAGT TTGAGGTAAG CACTGACTGC CATGAAGAAA ATGGAGAAGA GACTGGAGAC 
TTATCTATGA CCAATGATGA ATCATCCTGT GACATCATGG ACATGGACCA GGGGCAGAGG 
CTTAACAATG GAGCAAGCAC AAAAGAGAAC TTTGCATCTA CTGAAGAGGA AAGTTCAAAT 
GAGTCTCTAC TCATCAACAG CAGCAGTTCT CTAAATCCAG AACAGACATC TCGGAAAGAG 
CCTTTCCTTA AAGAAGGCTG TCTAAATGGT GAGGCCTCCA CAGACAGTTT TGAAGGAATA 
CCAGTTCTGG AGTGTCAGAA TGGCAAAGCT GAAGTAGTTT CTTGCTGTGG CAGTGGAGAT 
AAATGTAGTT CTGAACAAAA GACTCTCCTG GAGGACCACT CAAAAGAAAA ACCAGAAACT 
TCAAATGAAA ATCATGGAGA TGATCCTGAG AAACTAGAGG CACTGGAATG TAGCACTAAC 
GAGAAGTTAG AGCCTGGCCC TGATGTAGAG GTTAAAGATG CAGAACTGGA TAAAGAAGGT 
GCTTCTAAAG TAAAGAAATA TCGTAAGTTA ATTTTAGAAC AGGCAAAAAC AACAAGCCTG 
GAACTGGTTC CAGAAGAGCC ATCTGAGCCT GTGCCACCTC TTATAGTTGA TCATGAGAGA 
TTGAAGAAAT TGCTTGATTT GTTGGTAGAT AAAAGCAACA ATCTGGCAGT TGATCAACTT 
GAGAGATTAT ATTCTCTTCT TAGTCAGTGC ATCTACCGTC ACCGTAAAGA TTATGACAAA 
TCACAACTTG TAGAGGAGAT GGAAAGAACA GTTCATATGT TTGAGACATT CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050620.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050682.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBu08668
Clone ID Related Accession (Same CDS sequence)	XM_006050620.1 , XM_006050620.2
Accession Version	XM_006050620.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050620.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT GGATATTGAA GAGGTTTCAG ATTATCTTGA AGTAATCAAG 
GAACCAATGG ACTTATCAAC AGTAATAACT AAAATTGATA AGCATAATTA CCTGACTGCT 
AAGGATTTCC TAAAAGATAT TGACCTCATC TGTAGCAATG CTTTGGAGTA CAATCCAGAT 
AAGGACCCAG GAGACAAAAT AATTAGGCAC AGGGCTTGTA CTCTGAAGGA TACTGCACAT 
GCCATCATTG CAGCTGAGTT GGATCCAGAA TTTAATAAAC TCTGTGAAGA AATTAAGGAA 
GCAAGAATAA AAAGAGGCTT ATCGGTAACA GCAGAACAAA TAAATCCTCA TGGTACTGGA 
GCTCGAAAGA CAGAAACTAG AGTTGAAGAG GCATTTCGGC ACAAACAAAG AAATCCAATG 
GATGTCTGGC ACAGTTCTGC AAATAAATGT GCATTTCGAG TTCGGAGAAA ATCACGGCGG 
CGATCACAGT GGGGTAAAGG AATTATTAAG AAAAGGAAAG TCAATAATTT AAAAAAAGAT 
GAAGAGGACA CCAAATTTAC TGACTATGAG AATCATATGG AGGACAGGAA ATTGCTCGAA 
AATGGAGAGT TTGAGGTAAG CACTGACTGC CATGAAGAAA ATGGAGAAGA GACTGGAGAC 
TTATCTATGA CCAATGATGA ATCATCCTGT GACATCATGG ACATGGACCA GGGGCAGAGG 
CTTAACAATG GAGCAAGCAC AAAAGAGAAC TTTGCATCTA CTGAAGAGGA AAGTTCAAAT 
GAGTCTCTAC TCATCAACAG CAGCAGTTCT CTAAATCCAG AACAGACATC TCGGAAAGAG 
CCTTTCCTTA AAGAAGGCTG TCTAAATGGT GAGGCCTCCA CAGACAGTTT TGAAGGAATA 
CCAGTTCTGG AGTGTCAGAA TGGCAAAGCT GAAGTAGTTT CTTGCTGTGG CAGTGGAGAT 
AAATGTAGTT CTGAACAAAA GACTCTCCTG GAGGACCACT CAAAAGAAAA ACCAGAAACT 
TCAAATGAAA ATCATGGAGA TGATCCTGAG AAACTAGAGG CACTGGAATG TAGCACTAAC 
GAGAAGTTAG AGCCTGGCCC TGATGTAGAG GTTAAAGATG CAGAACTGGA TAAAGAAGGT 
GCTTCTAAAG TAAAGAAATA TCGTAAGTTA ATTTTAGAAC AGGCAAAAAC AACAAGCCTG 
GAACTGGTTC CAGAAGAGCC ATCTGAGCCT GTGCCACCTC TTATAGTTGA TCATGAGAGA 
TTGAAGAAAT TGCTTGATTT GTTGGTAGAT AAAAGCAACA ATCTGGCAGT TGATCAACTT 
GAGAGATTAT ATTCTCTTCT TAGTCAGTGC ATCTACCGTC ACCGTAAAGA TTATGACAAA 
TCACAACTTG TAGAGGAGAT GGAAAGAACA GTTCATATGT TTGAGACATT CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050682.1
CDS	319..4695
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050620.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050620.2

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT GGATATTGAA GAGGTTTCAG ATTATCTTGA AGTAATCAAG 
GAACCAATGG ACTTATCAAC AGTAATAACT AAAATTGATA AGCATAATTA CCTGACTGCT 
AAGGATTTCC TAAAAGATAT TGACCTCATC TGTAGCAATG CTTTGGAGTA CAATCCAGAT 
AAGGACCCAG GAGACAAAAT AATTAGGCAC AGGGCTTGTA CTCTGAAGGA TACTGCACAT 
GCCATCATTG CAGCTGAGTT GGATCCAGAA TTTAATAAAC TCTGTGAAGA AATTAAGGAA 
GCAAGAATAA AAAGAGGCTT ATCGGTAACA GCAGAACAAA TAAATCCTCA TGGTACTGGA 
GCTCGAAAGA CAGAAACTAG AGTTGAAGAG GCATTTCGGC ACAAACAAAG AAATCCAATG 
GATGTCTGGC ACAGTTCTGC AAATAAATGT GCATTTCGAG TTCGGAGAAA ATCACGGCGG 
CGATCACAGT GGGGTAAAGG AATTATTAAG AAAAGGAAAG TCAATAATTT AAAAAAAGAT 
GAAGAGGACA CCAAATTTAC TGACTATGAG AATCATATGG AGGACAGGAA ATTGCTCGAA 
AATGGAGAGT TTGAGGTAAG CACTGACTGC CATGAAGAAA ATGGAGAAGA GACTGGAGAC 
TTATCTATGA CCAATGATGA ATCATCCTGT GACATCATGG ACATGGACCA GGGGCAGAGG 
CTTAACAATG GAGCAAGCAC AAAAGAGAAC TTTGCATCTA CTGAAGAGGA AAGTTCAAAT 
GAGTCTCTAC TCATCAACAG CAGCAGTTCT CTAAATCCAG AACAGACATC TCGGAAAGAG 
CCTTTCCTTA AAGAAGGCTG TCTAAATGGT GAGGCCTCCA CAGACAGTTT TGAAGGAATA 
CCAGTTCTGG AGTGTCAGAA TGGCAAAGCT GAAGTAGTTT CTTGCTGTGG CAGTGGAGAT 
AAATGTAGTT CTGAACAAAA GACTCTCCTG GAGGACCACT CAAAAGAAAA ACCAGAAACT 
TCAAATGAAA ATCATGGAGA TGATCCTGAG AAACTAGAGG CACTGGAATG TAGCACTAAC 
GAGAAGTTAG AGCCTGGCCC TGATGTAGAG GTTAAAGATG CAGAACTGGA TAAAGAAGGT 
GCTTCTAAAG TAAAGAAATA TCGTAAGTTA ATTTTAGAAC AGGCAAAAAC AACAAGCCTG 
GAACTGGTTC CAGAAGAGCC ATCTGAGCCT GTGCCACCTC TTATAGTTGA TCATGAGAGA 
TTGAAGAAAT TGCTTGATTT GTTGGTAGAT AAAAGCAACA ATCTGGCAGT TGATCAACTT 
GAGAGATTAT ATTCTCTTCT TAGTCAGTGC ATCTACCGTC ACCGTAAAGA TTATGACAAA 
TCACAACTTG TAGAGGAGAT GGAAAGAACA GTTCATATGT TTGAGACATT CCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050621.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050683.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X8, mRNA.

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CloneID	OBu08669
Clone ID Related Accession (Same CDS sequence)	XM_006050621.2 , XM_006050621.1
Accession Version	XM_006050621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006050621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT TTCAGATTAT CTTGAAGTAA TCAAGGAACC AATGGACTTA 
TCAACAGTAA TAACTAAAAT TGATAAGCAT AATTACCTGA CTGCTAAGGA TTTCCTAAAA 
GATATTGACC TCATCTGTAG CAATGCTTTG GAGTACAATC CAGATAAGGA CCCAGGAGAC 
AAAATAATTA GGCACAGGGC TTGTACTCTG AAGGATACTG CACATGCCAT CATTGCAGCT 
GAGTTGGATC CAGAATTTAA TAAACTCTGT GAAGAAATTA AGGAAGCAAG AATAAAAAGA 
GGCTTATCGG TAACAGCAGA ACAAATAAAT CCTCATGGTA CTGGAGCTCG AAAGACAGAA 
ACTAGAGTTG AAGAGGCATT TCGGCACAAA CAAAGAAATC CAATGGATGT CTGGCACAGT 
TCTGCAAATA AATGTGCATT TCGAGTTCGG AGAAAATCAC GGCGGCGATC ACAGTGGGGT 
AAAGGAATTA TTAAGAAAAG GAAAGTCAAT AATTTAAAAA AAGATGAAGA GGACACCAAA 
TTTACTGACT ATGAGAATCA TATGGAGGAC AGGAAATTGC TCGAAAATGG AGAGTTTGAG 
GTAAGCACTG ACTGCCATGA AGAAAATGGA GAAGAGACTG GAGACTTATC TATGACCAAT 
GATGAATCAT CCTGTGACAT CATGGACATG GACCAGGGGC AGAGGCTTAA CAATGGAGCA 
AGCACAAAAG AGAACTTTGC ATCTACTGAA GAGGAAAGTT CAAATGAGTC TCTACTCATC 
AACAGCAGCA GTTCTCTAAA TCCAGAACAG ACATCTCGGA AAGAGCCTTT CCTTAAAGAA 
GGCTGTCTAA ATGGTGAGGC CTCCACAGAC AGTTTTGAAG GAATACCAGT TCTGGAGTGT 
CAGAATGGCA AAGCTGAAGT AGTTTCTTGC TGTGGCAGTG GAGATAAATG TAGTTCTGAA 
CAAAAGACTC TCCTGGAGGA CCACTCAAAA GAAAAACCAG AAACTTCAAA TGAAAATCAT 
GGAGATGATC CTGAGAAACT AGAGGCACTG GAATGTAGCA CTAACGAGAA GTTAGAGCCT 
GGCCCTGATG TAGAGGTTAA AGATGCAGAA CTGGATAAAG AAGGTGCTTC TAAAGTAAAG 
AAATATCGTA AGTTAATTTT AGAACAGGCA AAAACAACAA GCCTGGAACT GGTTCCAGAA 
GAGCCATCTG AGCCTGTGCC ACCTCTTATA GTTGATCATG AGAGATTGAA GAAATTGCTT 
GATTTGTTGG TAGATAAAAG CAACAATCTG GCAGTTGATC AACTTGAGAG ATTATATTCT 
CTTCTTAGTC AGTGCATCTA CCGTCACCGT AAAGATTATG ACAAATCACA ACTTGTAGAG 
GAGATGGAAA GAACAGTTCA TATGTTTGAG ACATTCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050683.1
CDS	320..4681
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050621.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050621.2

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT TTCAGATTAT CTTGAAGTAA TCAAGGAACC AATGGACTTA 
TCAACAGTAA TAACTAAAAT TGATAAGCAT AATTACCTGA CTGCTAAGGA TTTCCTAAAA 
GATATTGACC TCATCTGTAG CAATGCTTTG GAGTACAATC CAGATAAGGA CCCAGGAGAC 
AAAATAATTA GGCACAGGGC TTGTACTCTG AAGGATACTG CACATGCCAT CATTGCAGCT 
GAGTTGGATC CAGAATTTAA TAAACTCTGT GAAGAAATTA AGGAAGCAAG AATAAAAAGA 
GGCTTATCGG TAACAGCAGA ACAAATAAAT CCTCATGGTA CTGGAGCTCG AAAGACAGAA 
ACTAGAGTTG AAGAGGCATT TCGGCACAAA CAAAGAAATC CAATGGATGT CTGGCACAGT 
TCTGCAAATA AATGTGCATT TCGAGTTCGG AGAAAATCAC GGCGGCGATC ACAGTGGGGT 
AAAGGAATTA TTAAGAAAAG GAAAGTCAAT AATTTAAAAA AAGATGAAGA GGACACCAAA 
TTTACTGACT ATGAGAATCA TATGGAGGAC AGGAAATTGC TCGAAAATGG AGAGTTTGAG 
GTAAGCACTG ACTGCCATGA AGAAAATGGA GAAGAGACTG GAGACTTATC TATGACCAAT 
GATGAATCAT CCTGTGACAT CATGGACATG GACCAGGGGC AGAGGCTTAA CAATGGAGCA 
AGCACAAAAG AGAACTTTGC ATCTACTGAA GAGGAAAGTT CAAATGAGTC TCTACTCATC 
AACAGCAGCA GTTCTCTAAA TCCAGAACAG ACATCTCGGA AAGAGCCTTT CCTTAAAGAA 
GGCTGTCTAA ATGGTGAGGC CTCCACAGAC AGTTTTGAAG GAATACCAGT TCTGGAGTGT 
CAGAATGGCA AAGCTGAAGT AGTTTCTTGC TGTGGCAGTG GAGATAAATG TAGTTCTGAA 
CAAAAGACTC TCCTGGAGGA CCACTCAAAA GAAAAACCAG AAACTTCAAA TGAAAATCAT 
GGAGATGATC CTGAGAAACT AGAGGCACTG GAATGTAGCA CTAACGAGAA GTTAGAGCCT 
GGCCCTGATG TAGAGGTTAA AGATGCAGAA CTGGATAAAG AAGGTGCTTC TAAAGTAAAG 
AAATATCGTA AGTTAATTTT AGAACAGGCA AAAACAACAA GCCTGGAACT GGTTCCAGAA 
GAGCCATCTG AGCCTGTGCC ACCTCTTATA GTTGATCATG AGAGATTGAA GAAATTGCTT 
GATTTGTTGG TAGATAAAAG CAACAATCTG GCAGTTGATC AACTTGAGAG ATTATATTCT 
CTTCTTAGTC AGTGCATCTA CCGTCACCGT AAAGATTATG ACAAATCACA ACTTGTAGAG 
GAGATGGAAA GAACAGTTCA TATGTTTGAG ACATTCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050621.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050683.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X4, mRNA.

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CloneID	OBu08669
Clone ID Related Accession (Same CDS sequence)	XM_006050621.2 , XM_006050621.1
Accession Version	XM_006050621.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784308.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT TTCAGATTAT CTTGAAGTAA TCAAGGAACC AATGGACTTA 
TCAACAGTAA TAACTAAAAT TGATAAGCAT AATTACCTGA CTGCTAAGGA TTTCCTAAAA 
GATATTGACC TCATCTGTAG CAATGCTTTG GAGTACAATC CAGATAAGGA CCCAGGAGAC 
AAAATAATTA GGCACAGGGC TTGTACTCTG AAGGATACTG CACATGCCAT CATTGCAGCT 
GAGTTGGATC CAGAATTTAA TAAACTCTGT GAAGAAATTA AGGAAGCAAG AATAAAAAGA 
GGCTTATCGG TAACAGCAGA ACAAATAAAT CCTCATGGTA CTGGAGCTCG AAAGACAGAA 
ACTAGAGTTG AAGAGGCATT TCGGCACAAA CAAAGAAATC CAATGGATGT CTGGCACAGT 
TCTGCAAATA AATGTGCATT TCGAGTTCGG AGAAAATCAC GGCGGCGATC ACAGTGGGGT 
AAAGGAATTA TTAAGAAAAG GAAAGTCAAT AATTTAAAAA AAGATGAAGA GGACACCAAA 
TTTACTGACT ATGAGAATCA TATGGAGGAC AGGAAATTGC TCGAAAATGG AGAGTTTGAG 
GTAAGCACTG ACTGCCATGA AGAAAATGGA GAAGAGACTG GAGACTTATC TATGACCAAT 
GATGAATCAT CCTGTGACAT CATGGACATG GACCAGGGGC AGAGGCTTAA CAATGGAGCA 
AGCACAAAAG AGAACTTTGC ATCTACTGAA GAGGAAAGTT CAAATGAGTC TCTACTCATC 
AACAGCAGCA GTTCTCTAAA TCCAGAACAG ACATCTCGGA AAGAGCCTTT CCTTAAAGAA 
GGCTGTCTAA ATGGTGAGGC CTCCACAGAC AGTTTTGAAG GAATACCAGT TCTGGAGTGT 
CAGAATGGCA AAGCTGAAGT AGTTTCTTGC TGTGGCAGTG GAGATAAATG TAGTTCTGAA 
CAAAAGACTC TCCTGGAGGA CCACTCAAAA GAAAAACCAG AAACTTCAAA TGAAAATCAT 
GGAGATGATC CTGAGAAACT AGAGGCACTG GAATGTAGCA CTAACGAGAA GTTAGAGCCT 
GGCCCTGATG TAGAGGTTAA AGATGCAGAA CTGGATAAAG AAGGTGCTTC TAAAGTAAAG 
AAATATCGTA AGTTAATTTT AGAACAGGCA AAAACAACAA GCCTGGAACT GGTTCCAGAA 
GAGCCATCTG AGCCTGTGCC ACCTCTTATA GTTGATCATG AGAGATTGAA GAAATTGCTT 
GATTTGTTGG TAGATAAAAG CAACAATCTG GCAGTTGATC AACTTGAGAG ATTATATTCT 
CTTCTTAGTC AGTGCATCTA CCGTCACCGT AAAGATTATG ACAAATCACA ACTTGTAGAG 
GAGATGGAAA GAACAGTTCA TATGTTTGAG ACATTCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050683.1
CDS	367..4728
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050621.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050621.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT TTCAGATTAT CTTGAAGTAA TCAAGGAACC AATGGACTTA 
TCAACAGTAA TAACTAAAAT TGATAAGCAT AATTACCTGA CTGCTAAGGA TTTCCTAAAA 
GATATTGACC TCATCTGTAG CAATGCTTTG GAGTACAATC CAGATAAGGA CCCAGGAGAC 
AAAATAATTA GGCACAGGGC TTGTACTCTG AAGGATACTG CACATGCCAT CATTGCAGCT 
GAGTTGGATC CAGAATTTAA TAAACTCTGT GAAGAAATTA AGGAAGCAAG AATAAAAAGA 
GGCTTATCGG TAACAGCAGA ACAAATAAAT CCTCATGGTA CTGGAGCTCG AAAGACAGAA 
ACTAGAGTTG AAGAGGCATT TCGGCACAAA CAAAGAAATC CAATGGATGT CTGGCACAGT 
TCTGCAAATA AATGTGCATT TCGAGTTCGG AGAAAATCAC GGCGGCGATC ACAGTGGGGT 
AAAGGAATTA TTAAGAAAAG GAAAGTCAAT AATTTAAAAA AAGATGAAGA GGACACCAAA 
TTTACTGACT ATGAGAATCA TATGGAGGAC AGGAAATTGC TCGAAAATGG AGAGTTTGAG 
GTAAGCACTG ACTGCCATGA AGAAAATGGA GAAGAGACTG GAGACTTATC TATGACCAAT 
GATGAATCAT CCTGTGACAT CATGGACATG GACCAGGGGC AGAGGCTTAA CAATGGAGCA 
AGCACAAAAG AGAACTTTGC ATCTACTGAA GAGGAAAGTT CAAATGAGTC TCTACTCATC 
AACAGCAGCA GTTCTCTAAA TCCAGAACAG ACATCTCGGA AAGAGCCTTT CCTTAAAGAA 
GGCTGTCTAA ATGGTGAGGC CTCCACAGAC AGTTTTGAAG GAATACCAGT TCTGGAGTGT 
CAGAATGGCA AAGCTGAAGT AGTTTCTTGC TGTGGCAGTG GAGATAAATG TAGTTCTGAA 
CAAAAGACTC TCCTGGAGGA CCACTCAAAA GAAAAACCAG AAACTTCAAA TGAAAATCAT 
GGAGATGATC CTGAGAAACT AGAGGCACTG GAATGTAGCA CTAACGAGAA GTTAGAGCCT 
GGCCCTGATG TAGAGGTTAA AGATGCAGAA CTGGATAAAG AAGGTGCTTC TAAAGTAAAG 
AAATATCGTA AGTTAATTTT AGAACAGGCA AAAACAACAA GCCTGGAACT GGTTCCAGAA 
GAGCCATCTG AGCCTGTGCC ACCTCTTATA GTTGATCATG AGAGATTGAA GAAATTGCTT 
GATTTGTTGG TAGATAAAAG CAACAATCTG GCAGTTGATC AACTTGAGAG ATTATATTCT 
CTTCTTAGTC AGTGCATCTA CCGTCACCGT AAAGATTATG ACAAATCACA ACTTGTAGAG 
GAGATGGAAA GAACAGTTCA TATGTTTGAG ACATTCCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_006050622.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006050684.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X5, mRNA.

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CloneID	OBu08670
Clone ID Related Accession (Same CDS sequence)	XM_006050622.1
Accession Version	XM_006050622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784308.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACAAA GTTCAGAATC TCAAGAGGAA GATGGAGATA TAGAGGTTGA AGAGGCAGAA 
GGAGAAGAAA ATGATAGGCC ATATAATTTG AGACAGAGAA AAACAGTGGA TCGATACCAA 
GCACCTCCAA TAGTACCAGC TCATCAAAAA AAGAGGGAAA ATACCCTTTT TGATATTCAC 
AGATCTCCAG CAAGAAGAAG TCATATCAGG AGAAAGAAGC ATGCTATTCA CAGTAGTGAC 
ACAACGTCTT CTGATGAAGA ACGATTTGAA AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA 
AGAAATAGAT GTTTGCCTAT GAACTTAAGA GCAGAAGATT TAGCTAGTGG TATTCTCCGA 
GAACGTGTTA AAGTGGGTGC AAGCTTGGCT GATGTTGATC CAATGAATAT TGATAAATCA 
GTGCGGTTTG ATAGCATAGG TGGATTGAGT CATCATATTC ATGCACTAAA GGAAATGGTA 
GTATTCCCAC TTTTATATCC AGAAATTTTT GAAAAATTTA AAATTCAGCC TCCAAGAGGC 
TGTTTGTTTT ATGGTCCTCC TGGCACAGGT AAAACCTTGG TCGCCAGAGC GTTAGCTAAC 
GAGTGCAGTC AGGGAGACAA AAAGGTGGCT TTTTTTATGC GAAAAGGAGC GGATTGTCTG 
AGTAAGTGGG TTGGTGAATC TGAGCGGCAG CTTAGGCTTC TTTTTGATCA GGCATATTTG 
ATGAGACCTT CTATAATATT TTTTGATGAA ATAGATGGAT TAGCTCCAGT TCGCTCTAGC 
AGGCAAGATC AGATCCACAG CTCTATAGTG TCAACCCTCC TTGCTCTTAT GGATGGATTA 
GACAATAGGG GCGAAATTGT TGTTATTGGT GCTACAAATA GACTTGATTC TATAGATCCT 
GCTCTTAGGA GACCTGGTCG TTTTGACAGA GAATTTCTTT TCAACCTTCC AGATCAAAAG 
GCAAGAAAAC ACATTTTACA GATCCATACC AGGGACTGGA ATCCAAAATT GTCAGATGCT 
TTTTTAGGTG AATTGGCTGA AAAATGTGTT GGCTATTGTG GAGCTGATAT CAAGGCCCTG 
TGCACTGAAG CTGCCCTGAT TGCCCTCCGG AGGCGTTATC CCCAGATCTA TGCTAGCAGT 
CATAAACTGC AGCTAGATGT TTCCTCAATC GTGCTCAACG CCCAAGATTT TTATCATGCA 
ATGCAGAATA TTGTGCCTGC TTCCCAACGT GCTGTCATGT CTTCGGGACA TGCACTGTCT 
CCCATCATAA AACCACTGCT GGAAAGAAGC TTCAACAACA TCCTAGCCGT CCTGCAAAAA 
GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT GACAAGAAAG AAGATATAGA AACTTTGATT 
TTAGATGACA GTGAAGATGA GAATGCTGTA TCAATTTTCG AGACAAGTTG TCACTCAGGA 
TCACCAAAGA AACAGTCATC AGCTGCTGCT GTACATAAAC CTTATCTTCA TTTTACAATG 
TCACCATATC ATCAGCCAAC CTCTTACAGG CCAAGGTTAT TGCTGTCTGG AGAACGAGGC 
TCAGGTCAAA CTTCTCACCT TGCTCCAGCA CTTTTGCACA CTTTAGAAAA ATTCTCTGTG 
CATAGACTAG ATCTCCCAGC ACTTTATTCA GTTAGTGCCA AAACACCTGA AGAATCGTGT 
GCACAGATTT TTCGTGAAGC TCGAAGAACA GTACCTAGTA TTGTTTACAT GCCTCACATT 
GGTGATTGGT GGGAAGCTGT CAGTGAAACT GTGAGAGCAA CTTTTCTGAC TCTGCTACAA 
GATATACCGT CATTTTCACC CATATTTCTG TTGTCTACCT CTGAAACCAT GTACAGTGAA 
CTGCCTGAAG AGGTTAAATG TATCTTTAGA ATACAGTACG AAGAGGTCTT GTATATTCAA 
AGGCCTATTG AAGAGGACAG AAGAAAATTT TTCCAAGAAC TGATTCTCAA TCAGGCCTCA 
ATGGCTCCAC CACGAAGGAA ACACACTGGT CTTTGTGCAA TGGAGGTACT TCCACTTGCA 
TTACCTTCTC CACCTCGTCA GTTATCAGAA TCAGAGAAAA TTCGGATGGA GGACCAAGAG 
GAAAATACTT TAAGAGAGTT GCGGTTGTTT CTCAGGGATG TAACCAAGAG GCTGGCGACA 
GATAAACGCT TTAACATCTT CAGCAAACCG GTGGATATTG AAGAGGTTTC AGATTATCTT 
GAAGTAATCA AGGAACCAAT GGACTTATCA ACAGTAATAA CTAAAATTGA TAAGCATAAT 
TACCTGACTG CTAAGGATTT CCTAAAAGAT ATTGACCTCA TCTGTAGCAA TGCTTTGGAG 
TACAATCCAG ATAAGGACCC AGGAGACAAA ATAATTAGGC ACAGGGCTTG TACTCTGAAG 
GATACTGCAC ATGCCATCAT TGCAGCTGAG TTGGATCCAG AATTTAATAA ACTCTGTGAA 
GAAATTAAGG AAGCAAGAAT AAAAAGAGGC TTATCGGTAA CAGCAGAACA AATAAATCCT 
CATGGTACTG GAGCTCGAAA GACAGAAACT AGAGTTGAAG AGGCATTTCG GCACAAACAA 
AGAAATCCAA TGGATGTCTG GCACAGTTCT GCAAATAAAT GTGCATTTCG AGTTCGGAGA 
AAATCACGGC GGCGATCACA GTGGGGTAAA GGAATTATTA AGAAAAGGAA AGTCAATAAT 
TTAAAAAAAG ATGAAGAGGA CACCAAATTT ACTGACTATG AGAATCATAT GGAGGACAGG 
AAATTGCTCG AAAATGGAGA GTTTGAGGTA AGCACTGACT GCCATGAAGA AAATGGAGAA 
GAGACTGGAG ACTTATCTAT GACCAATGAT GAATCATCCT GTGACATCAT GGACATGGAC 
CAGGGGCAGA GGCTTAACAA TGGAGCAAGC ACAAAAGAGA ACTTTGCATC TACTGAAGAG 
GAAAGTTCAA ATGAGTCTCT ACTCATCAAC AGCAGCAGTT CTCTAAATCC AGAACAGACA 
TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC TGTCTAAATG GTGAGGCCTC CACAGACAGT 
TTTGAAGGAA TACCAGTTCT GGAGTGTCAG AATGGCAAAG CTGAAGTAGT TTCTTGCTGT 
GGCAGTGGAG ATAAATGTAG TTCTGAACAA AAGACTCTCC TGGAGGACCA CTCAAAAGAA 
AAACCAGAAA CTTCAAATGA AAATCATGGA GATGATCCTG AGAAACTAGA GGCACTGGAA 
TGTAGCACTA ACGAGAAGTT AGAGCCTGGC CCTGATGTAG AGGTTAAAGA TGCAGAACTG 
GATAAAGAAG GTGCTTCTAA AGTAAAGAAA TATCGTAAGT TAATTTTAGA ACAGGCAAAA 
ACAACAAGCC TGGAACTGGT TCCAGAAGAG CCATCTGAGC CTGTGCCACC TCTTATAGTT 
GATCATGAGA GATTGAAGAA ATTGCTTGAT TTGTTGGTAG ATAAAAGCAA CAATCTGGCA 
GTTGATCAAC TTGAGAGATT ATATTCTCTT CTTAGTCAGT GCATCTACCG TCACCGTAAA 
GATTATGACA AATCACAACT TGTAGAGGAG ATGGAAAGAA CAGTTCATAT GTTTGAGACA 
TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006050684.1
CDS	27..3635
Translation	MLQSSESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEEMERTVHMFETFL

Target ORF information:

RefSeq Version	XM_006050622.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006050622.1

ORF Insert Sequence:

ATGCTACAAA GTTCAGAATC TCAAGAGGAA GATGGAGATA TAGAGGTTGA AGAGGCAGAA 
GGAGAAGAAA ATGATAGGCC ATATAATTTG AGACAGAGAA AAACAGTGGA TCGATACCAA 
GCACCTCCAA TAGTACCAGC TCATCAAAAA AAGAGGGAAA ATACCCTTTT TGATATTCAC 
AGATCTCCAG CAAGAAGAAG TCATATCAGG AGAAAGAAGC ATGCTATTCA CAGTAGTGAC 
ACAACGTCTT CTGATGAAGA ACGATTTGAA AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA 
AGAAATAGAT GTTTGCCTAT GAACTTAAGA GCAGAAGATT TAGCTAGTGG TATTCTCCGA 
GAACGTGTTA AAGTGGGTGC AAGCTTGGCT GATGTTGATC CAATGAATAT TGATAAATCA 
GTGCGGTTTG ATAGCATAGG TGGATTGAGT CATCATATTC ATGCACTAAA GGAAATGGTA 
GTATTCCCAC TTTTATATCC AGAAATTTTT GAAAAATTTA AAATTCAGCC TCCAAGAGGC 
TGTTTGTTTT ATGGTCCTCC TGGCACAGGT AAAACCTTGG TCGCCAGAGC GTTAGCTAAC 
GAGTGCAGTC AGGGAGACAA AAAGGTGGCT TTTTTTATGC GAAAAGGAGC GGATTGTCTG 
AGTAAGTGGG TTGGTGAATC TGAGCGGCAG CTTAGGCTTC TTTTTGATCA GGCATATTTG 
ATGAGACCTT CTATAATATT TTTTGATGAA ATAGATGGAT TAGCTCCAGT TCGCTCTAGC 
AGGCAAGATC AGATCCACAG CTCTATAGTG TCAACCCTCC TTGCTCTTAT GGATGGATTA 
GACAATAGGG GCGAAATTGT TGTTATTGGT GCTACAAATA GACTTGATTC TATAGATCCT 
GCTCTTAGGA GACCTGGTCG TTTTGACAGA GAATTTCTTT TCAACCTTCC AGATCAAAAG 
GCAAGAAAAC ACATTTTACA GATCCATACC AGGGACTGGA ATCCAAAATT GTCAGATGCT 
TTTTTAGGTG AATTGGCTGA AAAATGTGTT GGCTATTGTG GAGCTGATAT CAAGGCCCTG 
TGCACTGAAG CTGCCCTGAT TGCCCTCCGG AGGCGTTATC CCCAGATCTA TGCTAGCAGT 
CATAAACTGC AGCTAGATGT TTCCTCAATC GTGCTCAACG CCCAAGATTT TTATCATGCA 
ATGCAGAATA TTGTGCCTGC TTCCCAACGT GCTGTCATGT CTTCGGGACA TGCACTGTCT 
CCCATCATAA AACCACTGCT GGAAAGAAGC TTCAACAACA TCCTAGCCGT CCTGCAAAAA 
GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT GACAAGAAAG AAGATATAGA AACTTTGATT 
TTAGATGACA GTGAAGATGA GAATGCTGTA TCAATTTTCG AGACAAGTTG TCACTCAGGA 
TCACCAAAGA AACAGTCATC AGCTGCTGCT GTACATAAAC CTTATCTTCA TTTTACAATG 
TCACCATATC ATCAGCCAAC CTCTTACAGG CCAAGGTTAT TGCTGTCTGG AGAACGAGGC 
TCAGGTCAAA CTTCTCACCT TGCTCCAGCA CTTTTGCACA CTTTAGAAAA ATTCTCTGTG 
CATAGACTAG ATCTCCCAGC ACTTTATTCA GTTAGTGCCA AAACACCTGA AGAATCGTGT 
GCACAGATTT TTCGTGAAGC TCGAAGAACA GTACCTAGTA TTGTTTACAT GCCTCACATT 
GGTGATTGGT GGGAAGCTGT CAGTGAAACT GTGAGAGCAA CTTTTCTGAC TCTGCTACAA 
GATATACCGT CATTTTCACC CATATTTCTG TTGTCTACCT CTGAAACCAT GTACAGTGAA 
CTGCCTGAAG AGGTTAAATG TATCTTTAGA ATACAGTACG AAGAGGTCTT GTATATTCAA 
AGGCCTATTG AAGAGGACAG AAGAAAATTT TTCCAAGAAC TGATTCTCAA TCAGGCCTCA 
ATGGCTCCAC CACGAAGGAA ACACACTGGT CTTTGTGCAA TGGAGGTACT TCCACTTGCA 
TTACCTTCTC CACCTCGTCA GTTATCAGAA TCAGAGAAAA TTCGGATGGA GGACCAAGAG 
GAAAATACTT TAAGAGAGTT GCGGTTGTTT CTCAGGGATG TAACCAAGAG GCTGGCGACA 
GATAAACGCT TTAACATCTT CAGCAAACCG GTGGATATTG AAGAGGTTTC AGATTATCTT 
GAAGTAATCA AGGAACCAAT GGACTTATCA ACAGTAATAA CTAAAATTGA TAAGCATAAT 
TACCTGACTG CTAAGGATTT CCTAAAAGAT ATTGACCTCA TCTGTAGCAA TGCTTTGGAG 
TACAATCCAG ATAAGGACCC AGGAGACAAA ATAATTAGGC ACAGGGCTTG TACTCTGAAG 
GATACTGCAC ATGCCATCAT TGCAGCTGAG TTGGATCCAG AATTTAATAA ACTCTGTGAA 
GAAATTAAGG AAGCAAGAAT AAAAAGAGGC TTATCGGTAA CAGCAGAACA AATAAATCCT 
CATGGTACTG GAGCTCGAAA GACAGAAACT AGAGTTGAAG AGGCATTTCG GCACAAACAA 
AGAAATCCAA TGGATGTCTG GCACAGTTCT GCAAATAAAT GTGCATTTCG AGTTCGGAGA 
AAATCACGGC GGCGATCACA GTGGGGTAAA GGAATTATTA AGAAAAGGAA AGTCAATAAT 
TTAAAAAAAG ATGAAGAGGA CACCAAATTT ACTGACTATG AGAATCATAT GGAGGACAGG 
AAATTGCTCG AAAATGGAGA GTTTGAGGTA AGCACTGACT GCCATGAAGA AAATGGAGAA 
GAGACTGGAG ACTTATCTAT GACCAATGAT GAATCATCCT GTGACATCAT GGACATGGAC 
CAGGGGCAGA GGCTTAACAA TGGAGCAAGC ACAAAAGAGA ACTTTGCATC TACTGAAGAG 
GAAAGTTCAA ATGAGTCTCT ACTCATCAAC AGCAGCAGTT CTCTAAATCC AGAACAGACA 
TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC TGTCTAAATG GTGAGGCCTC CACAGACAGT 
TTTGAAGGAA TACCAGTTCT GGAGTGTCAG AATGGCAAAG CTGAAGTAGT TTCTTGCTGT 
GGCAGTGGAG ATAAATGTAG TTCTGAACAA AAGACTCTCC TGGAGGACCA CTCAAAAGAA 
AAACCAGAAA CTTCAAATGA AAATCATGGA GATGATCCTG AGAAACTAGA GGCACTGGAA 
TGTAGCACTA ACGAGAAGTT AGAGCCTGGC CCTGATGTAG AGGTTAAAGA TGCAGAACTG 
GATAAAGAAG GTGCTTCTAA AGTAAAGAAA TATCGTAAGT TAATTTTAGA ACAGGCAAAA 
ACAACAAGCC TGGAACTGGT TCCAGAAGAG CCATCTGAGC CTGTGCCACC TCTTATAGTT 
GATCATGAGA GATTGAAGAA ATTGCTTGAT TTGTTGGTAG ATAAAAGCAA CAATCTGGCA 
GTTGATCAAC TTGAGAGATT ATATTCTCTT CTTAGTCAGT GCATCTACCG TCACCGTAAA 
GATTATGACA AATCACAACT TGTAGAGGAG ATGGAAAGAA CAGTTCATAT GTTTGAGACA 
TTCCTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_025261500.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117285.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu46438
Clone ID Related Accession (Same CDS sequence)	XM_025261500.1
Accession Version	XM_025261500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT GGATATTGAA GAGGTTTCAG ATTATCTTGA AGTAATCAAG 
GAACCAATGG ACTTATCAAC AGTAATAACT AAAATTGATA AGCATAATTA CCTGACTGCT 
AAGGATTTCC TAAAAGATAT TGACCTCATC TGTAGCAATG CTTTGGAGTA CAATCCAGAT 
AAGGACCCAG GAGACAAAAT AATTAGGCAC AGGGCTTGTA CTCTGAAGGA TACTGCACAT 
GCCATCATTG CAGCTGAGTT GGATCCAGAA TTTAATAAAC TCTGTGAAGA AATTAAGGAA 
GCAAGAATAA AAAGAGGCTT ATCGGTAACA GCAGAACAAA TAAATCCTCA TGGTACTGGA 
GCTCGAAAGA CAGAAACTAG AGTTGAAGAG GCATTTCGGC ACAAACAAAG AAATCCAATG 
GATGTCTGGC ACAGTTCTGC AAATAAATGT GCATTTCGAG TTCGGAGAAA ATCACGGCGG 
CGATCACAGT GGGGTAAAGG AATTATTAAG AAAAGGAAAG TCAATAATTT AAAAAAAGAT 
GAAGAGGACA CCAAATTTAC TGACTATGAG AATCATATGG AGGACAGGAA ATTGCTCGAA 
AATGGAGAGT TTGAGGTAAG CACTGACTGC CATGAAGAAA ATGGAGAAGA GACTGGAGAC 
TTATCTATGA CCAATGATGA ATCATCCTGT GACATCATGG ACATGGACCA GGGGCAGAGG 
CTTAACAATG GAGCAAGCAC AAAAGAGAAC TTTGCATCTA CTGAAGAGGA AAGTTCAAAT 
GAGTCTCTAC TCATCAACAG CAGCAGTTCT CTAAATCCAG AACAGACATC TCGGAAAGAG 
CCTTTCCTTA AAGAAGGCTG TCTAAATGGT GAGGCCTCCA CAGACAGTTT TGAAGGAATA 
CCAGTTCTGG AGTGTCAGAA TGGCAAAGCT GAAGTAGTTT CTTGCTGTGG CAGTGGAGAT 
AAATGTAGTT CTGAACAAAA GACTCTCCTG GAGGACCACT CAAAAGAAAA ACCAGAAACT 
TCAAATGAAA ATCATGGAGA TGATCCTGAG AAACTAGAGG CACTGGAATG TAGCACTAAC 
GAGAAGTTAG AGCCTGGCCC TGATGTAGAG GTTAAAGATG CAGAACTGGA TAAAGAAGGT 
GCTTCTAAAG TAAAGAAATA TCGTAAGTTA ATTTTAGAAC AGGCAAAAAC AACAAGCCTG 
GAACTGGTTC CAGAAGAGCC ATCTGAGCCT GTGCCACCTC TTATAGTTGA TCATGAGAGA 
TTGAAGAAAT TGCTTGATTT GTTGGTAGAT AAAAGCAACA ATCTGGCAGT TGATCAACTT 
GAGAGATTAT ATTCTCTTCT TAGTCAGTGC ATCTACCGTC ACCGTAAAGA TTATGACAAA 
TCACAACTTG TAGAGAACAG AAGTAATCTG GGTTTCTTTG TTTATCAAAA CACAGAAATC 
CATGGTTGGC AGACACACGG AATAACATGG CGCAGTTCTC CCCTCCCTTG CTTCTCGGCG 
ACTTCACAGA TGATGCATAT CACGTGGGCC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117285.1
CDS	320..4795
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVENRSNLGFFVYQNTEIHGWQTHGITWRSSPLPCFSATSQMMHITWAC

Target ORF information:

RefSeq Version	XM_025261500.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261500.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGAATCTC AAGAGGAAGA TGGAGATATA GAGGTTGAAG AGGCAGAAGG AGAAGAAAAT 
GATAGGCCAT ATAATTTGAG ACAGAGAAAA ACAGTGGATC GATACCAAGC ACCTCCAATA 
GTACCAGCTC ATCAAAAAAA GAGGGAAAAT ACCCTTTTTG ATATTCACAG ATCTCCAGCA 
AGAAGAAGTC ATATCAGGAG AAAGAAGCAT GCTATTCACA GTAGTGACAC AACGTCTTCT 
GATGAAGAAC GATTTGAAAG AAGGAAATCA AAGAGCATGG CAAGAGCAAG AAATAGATGT 
TTGCCTATGA ACTTAAGAGC AGAAGATTTA GCTAGTGGTA TTCTCCGAGA ACGTGTTAAA 
GTGGGTGCAA GCTTGGCTGA TGTTGATCCA ATGAATATTG ATAAATCAGT GCGGTTTGAT 
AGCATAGGTG GATTGAGTCA TCATATTCAT GCACTAAAGG AAATGGTAGT ATTCCCACTT 
TTATATCCAG AAATTTTTGA AAAATTTAAA ATTCAGCCTC CAAGAGGCTG TTTGTTTTAT 
GGTCCTCCTG GCACAGGTAA AACCTTGGTC GCCAGAGCGT TAGCTAACGA GTGCAGTCAG 
GGAGACAAAA AGGTGGCTTT TTTTATGCGA AAAGGAGCGG ATTGTCTGAG TAAGTGGGTT 
GGTGAATCTG AGCGGCAGCT TAGGCTTCTT TTTGATCAGG CATATTTGAT GAGACCTTCT 
ATAATATTTT TTGATGAAAT AGATGGATTA GCTCCAGTTC GCTCTAGCAG GCAAGATCAG 
ATCCACAGCT CTATAGTGTC AACCCTCCTT GCTCTTATGG ATGGATTAGA CAATAGGGGC 
GAAATTGTTG TTATTGGTGC TACAAATAGA CTTGATTCTA TAGATCCTGC TCTTAGGAGA 
CCTGGTCGTT TTGACAGAGA ATTTCTTTTC AACCTTCCAG ATCAAAAGGC AAGAAAACAC 
ATTTTACAGA TCCATACCAG GGACTGGAAT CCAAAATTGT CAGATGCTTT TTTAGGTGAA 
TTGGCTGAAA AATGTGTTGG CTATTGTGGA GCTGATATCA AGGCCCTGTG CACTGAAGCT 
GCCCTGATTG CCCTCCGGAG GCGTTATCCC CAGATCTATG CTAGCAGTCA TAAACTGCAG 
CTAGATGTTT CCTCAATCGT GCTCAACGCC CAAGATTTTT ATCATGCAAT GCAGAATATT 
GTGCCTGCTT CCCAACGTGC TGTCATGTCT TCGGGACATG CACTGTCTCC CATCATAAAA 
CCACTGCTGG AAAGAAGCTT CAACAACATC CTAGCCGTCC TGCAAAAAGT GTTTCCTCAT 
GCTGAGATTA GCCAGAGTGA CAAGAAAGAA GATATAGAAA CTTTGATTTT AGATGACAGT 
GAAGATGAGA ATGCTGTATC AATTTTCGAG ACAAGTTGTC ACTCAGGATC ACCAAAGAAA 
CAGTCATCAG CTGCTGCTGT ACATAAACCT TATCTTCATT TTACAATGTC ACCATATCAT 
CAGCCAACCT CTTACAGGCC AAGGTTATTG CTGTCTGGAG AACGAGGCTC AGGTCAAACT 
TCTCACCTTG CTCCAGCACT TTTGCACACT TTAGAAAAAT TCTCTGTGCA TAGACTAGAT 
CTCCCAGCAC TTTATTCAGT TAGTGCCAAA ACACCTGAAG AATCGTGTGC ACAGATTTTT 
CGTGAAGCTC GAAGAACAGT ACCTAGTATT GTTTACATGC CTCACATTGG TGATTGGTGG 
GAAGCTGTCA GTGAAACTGT GAGAGCAACT TTTCTGACTC TGCTACAAGA TATACCGTCA 
TTTTCACCCA TATTTCTGTT GTCTACCTCT GAAACCATGT ACAGTGAACT GCCTGAAGAG 
GTTAAATGTA TCTTTAGAAT ACAGTACGAA GAGGTCTTGT ATATTCAAAG GCCTATTGAA 
GAGGACAGAA GAAAATTTTT CCAAGAACTG ATTCTCAATC AGGCCTCAAT GGCTCCACCA 
CGAAGGAAAC ACACTGGTCT TTGTGCAATG GAGGTACTTC CACTTGCATT ACCTTCTCCA 
CCTCGTCAGT TATCAGAATC AGAGAAAATT CGGATGGAGG ACCAAGAGGA AAATACTTTA 
AGAGAGTTGC GGTTGTTTCT CAGGGATGTA ACCAAGAGGC TGGCGACAGA TAAACGCTTT 
AACATCTTCA GCAAACCGGT GGATATTGAA GAGGTTTCAG ATTATCTTGA AGTAATCAAG 
GAACCAATGG ACTTATCAAC AGTAATAACT AAAATTGATA AGCATAATTA CCTGACTGCT 
AAGGATTTCC TAAAAGATAT TGACCTCATC TGTAGCAATG CTTTGGAGTA CAATCCAGAT 
AAGGACCCAG GAGACAAAAT AATTAGGCAC AGGGCTTGTA CTCTGAAGGA TACTGCACAT 
GCCATCATTG CAGCTGAGTT GGATCCAGAA TTTAATAAAC TCTGTGAAGA AATTAAGGAA 
GCAAGAATAA AAAGAGGCTT ATCGGTAACA GCAGAACAAA TAAATCCTCA TGGTACTGGA 
GCTCGAAAGA CAGAAACTAG AGTTGAAGAG GCATTTCGGC ACAAACAAAG AAATCCAATG 
GATGTCTGGC ACAGTTCTGC AAATAAATGT GCATTTCGAG TTCGGAGAAA ATCACGGCGG 
CGATCACAGT GGGGTAAAGG AATTATTAAG AAAAGGAAAG TCAATAATTT AAAAAAAGAT 
GAAGAGGACA CCAAATTTAC TGACTATGAG AATCATATGG AGGACAGGAA ATTGCTCGAA 
AATGGAGAGT TTGAGGTAAG CACTGACTGC CATGAAGAAA ATGGAGAAGA GACTGGAGAC 
TTATCTATGA CCAATGATGA ATCATCCTGT GACATCATGG ACATGGACCA GGGGCAGAGG 
CTTAACAATG GAGCAAGCAC AAAAGAGAAC TTTGCATCTA CTGAAGAGGA AAGTTCAAAT 
GAGTCTCTAC TCATCAACAG CAGCAGTTCT CTAAATCCAG AACAGACATC TCGGAAAGAG 
CCTTTCCTTA AAGAAGGCTG TCTAAATGGT GAGGCCTCCA CAGACAGTTT TGAAGGAATA 
CCAGTTCTGG AGTGTCAGAA TGGCAAAGCT GAAGTAGTTT CTTGCTGTGG CAGTGGAGAT 
AAATGTAGTT CTGAACAAAA GACTCTCCTG GAGGACCACT CAAAAGAAAA ACCAGAAACT 
TCAAATGAAA ATCATGGAGA TGATCCTGAG AAACTAGAGG CACTGGAATG TAGCACTAAC 
GAGAAGTTAG AGCCTGGCCC TGATGTAGAG GTTAAAGATG CAGAACTGGA TAAAGAAGGT 
GCTTCTAAAG TAAAGAAATA TCGTAAGTTA ATTTTAGAAC AGGCAAAAAC AACAAGCCTG 
GAACTGGTTC CAGAAGAGCC ATCTGAGCCT GTGCCACCTC TTATAGTTGA TCATGAGAGA 
TTGAAGAAAT TGCTTGATTT GTTGGTAGAT AAAAGCAACA ATCTGGCAGT TGATCAACTT 
GAGAGATTAT ATTCTCTTCT TAGTCAGTGC ATCTACCGTC ACCGTAAAGA TTATGACAAA 
TCACAACTTG TAGAGAACAG AAGTAATCTG GGTTTCTTTG TTTATCAAAA CACAGAAATC 
CATGGTTGGC AGACACACGG AATAACATGG CGCAGTTCTC CCCTCCCTTG CTTCTCGGCG 
ACTTCACAGA TGATGCATAT CACGTGGGCC TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_025261501.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117286.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X5, mRNA.

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CloneID	OBu46439
Clone ID Related Accession (Same CDS sequence)	XM_025261501.1
Accession Version	XM_025261501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGAAA 
TCCATGGTTG GCAGACACAC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117286.1
CDS	320..4726
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVEKSMVGRHTE

Target ORF information:

RefSeq Version	XM_025261501.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261501.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGAAA 
TCCATGGTTG GCAGACACAC GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_025261503.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117288.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBu46440
Clone ID Related Accession (Same CDS sequence)	XM_025261503.1
Accession Version	XM_025261503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGTCA GAAAAAGCTG TCGTTCCAGG AAAAACAGAT TTGAAAGTGT GAACCAGAGT 
TTGTTATTTG ATCAACTGGT AAATAGTACT GCTGAAGCTG TACTACAGGA AATGGACAAC 
ATAAACATTA GACGGAATCG TCGATCAGGA GAAGTAGAAC GGCTTCGAAT GTGGACAGAT 
ACAGAATTTG AAAACATGGA TATGTATTCA AGAGTGAAAA GGCGAAGAAA GTCACTGAGA 
AGAAATAGTT ATGGTATACA AAATCATCAT GAAGTCTCTA CTGAGGGTGA AGAAGAAGCT 
AGAACCTCTA TATTGCTTCC TTTGGAGAAA ATCAGTATAG AATCTCAAGA GGAAGATGGA 
GATATAGAGG TTGAAGAGGC AGAAGGAGAA GAAAATGATA GGCCATATAA TTTGAGACAG 
AGAAAAACAG TGGATCGATA CCAAGCACCT CCAATAGTAC CAGCTCATCA AAAAAAGAGG 
GAAAATACCC TTTTTGATAT TCACAGATCT CCAGCAAGAA GAAGTCATAT CAGGAGAAAG 
AAGCATGCTA TTCACAGTAG TGACACAACG TCTTCTGATG AAGAACGATT TGAAAGAAGG 
AAATCAAAGA GCATGGCAAG AGCAAGAAAT AGATGTTTGC CTATGAACTT AAGAGCAGAA 
GATTTAGCTA GTGGTATTCT CCGAGAACGT GTTAAAGTGG GTGCAAGCTT GGCTGATGTT 
GATCCAATGA ATATTGATAA ATCAGTGCGG TTTGATAGCA TAGGTGGATT GAGTCATCAT 
ATTCATGCAC TAAAGGAAAT GGTAGTATTC CCACTTTTAT ATCCAGAAAT TTTTGAAAAA 
TTTAAAATTC AGCCTCCAAG AGGCTGTTTG TTTTATGGTC CTCCTGGCAC AGGTAAAACC 
TTGGTCGCCA GAGCGTTAGC TAACGAGTGC AGTCAGGGAG ACAAAAAGGT GGCTTTTTTT 
ATGCGAAAAG GAGCGGATTG TCTGAGTAAG TGGGTTGGTG AATCTGAGCG GCAGCTTAGG 
CTTCTTTTTG ATCAGGCATA TTTGATGAGA CCTTCTATAA TATTTTTTGA TGAAATAGAT 
GGATTAGCTC CAGTTCGCTC TAGCAGGCAA GATCAGATCC ACAGCTCTAT AGTGTCAACC 
CTCCTTGCTC TTATGGATGG ATTAGACAAT AGGGGCGAAA TTGTTGTTAT TGGTGCTACA 
AATAGACTTG ATTCTATAGA TCCTGCTCTT AGGAGACCTG GTCGTTTTGA CAGAGAATTT 
CTTTTCAACC TTCCAGATCA AAAGGCAAGA AAACACATTT TACAGATCCA TACCAGGGAC 
TGGAATCCAA AATTGTCAGA TGCTTTTTTA GGTGAATTGG CTGAAAAATG TGTTGGCTAT 
TGTGGAGCTG ATATCAAGGC CCTGTGCACT GAAGCTGCCC TGATTGCCCT CCGGAGGCGT 
TATCCCCAGA TCTATGCTAG CAGTCATAAA CTGCAGCTAG ATGTTTCCTC AATCGTGCTC 
AACGCCCAAG ATTTTTATCA TGCAATGCAG AATATTGTGC CTGCTTCCCA ACGTGCTGTC 
ATGTCTTCGG GACATGCACT GTCTCCCATC ATAAAACCAC TGCTGGAAAG AAGCTTCAAC 
AACATCCTAG CCGTCCTGCA AAAAGTGTTT CCTCATGCTG AGATTAGCCA GAGTGACAAG 
AAAGAAGATA TAGAAACTTT GATTTTAGAT GACAGTGAAG ATGAGAATGC TGTATCAATT 
TTCGAGACAA GTTGTCACTC AGGATCACCA AAGAAACAGT CATCAGCTGC TGCTGTACAT 
AAACCTTATC TTCATTTTAC AATGTCACCA TATCATCAGC CAACCTCTTA CAGGCCAAGG 
TTATTGCTGT CTGGAGAACG AGGCTCAGGT CAAACTTCTC ACCTTGCTCC AGCACTTTTG 
CACACTTTAG AAAAATTCTC TGTGCATAGA CTAGATCTCC CAGCACTTTA TTCAGTTAGT 
GCCAAAACAC CTGAAGAATC GTGTGCACAG ATTTTTCGTG AAGCTCGAAG AACAGTACCT 
AGTATTGTTT ACATGCCTCA CATTGGTGAT TGGTGGGAAG CTGTCAGTGA AACTGTGAGA 
GCAACTTTTC TGACTCTGCT ACAAGATATA CCGTCATTTT CACCCATATT TCTGTTGTCT 
ACCTCTGAAA CCATGTACAG TGAACTGCCT GAAGAGGTTA AATGTATCTT TAGAATACAG 
TACGAAGAGG TCTTGTATAT TCAAAGGCCT ATTGAAGAGG ACAGAAGAAA ATTTTTCCAA 
GAACTGATTC TCAATCAGGC CTCAATGGCT CCACCACGAA GGAAACACAC TGGTCTTTGT 
GCAATGGAGG TACTTCCACT TGCATTACCT TCTCCACCTC GTCAGTTATC AGAATCAGAG 
AAAATTCGGA TGGAGGACCA AGAGGAAAAT ACTTTAAGAG AGTTGCGGTT GTTTCTCAGG 
GATGTAACCA AGAGGCTGGC GACAGATAAA CGCTTTAACA TCTTCAGCAA ACCGGTGGAT 
ATTGAAGAGG TTTCAGATTA TCTTGAAGTA ATCAAGGAAC CAATGGACTT ATCAACAGTA 
ATAACTAAAA TTGATAAGCA TAATTACCTG ACTGCTAAGG ATTTCCTAAA AGATATTGAC 
CTCATCTGTA GCAATGCTTT GGAGTACAAT CCAGATAAGG ACCCAGGAGA CAAAATAATT 
AGGCACAGGG CTTGTACTCT GAAGGATACT GCACATGCCA TCATTGCAGC TGAGTTGGAT 
CCAGAATTTA ATAAACTCTG TGAAGAAATT AAGGAAGCAA GAATAAAAAG AGGCTTATCG 
GTAACAGCAG AACAAATAAA TCCTCATGGT ACTGGAGCTC GAAAGACAGA AACTAGAGTT 
GAAGAGGCAT TTCGGCACAA ACAAAGAAAT CCAATGGATG TCTGGCACAG TTCTGCAAAT 
AAATGTGCAT TTCGAGTTCG GAGAAAATCA CGGCGGCGAT CACAGTGGGG TAAAGGAATT 
ATTAAGAAAA GGAAAGTCAA TAATTTAAAA AAAGATGAAG AGGACACCAA ATTTACTGAC 
TATGAGAATC ATATGGAGGA CAGGAAATTG CTCGAAAATG GAGAGTTTGA GGTAAGCACT 
GACTGCCATG AAGAAAATGG AGAAGAGACT GGAGACTTAT CTATGACCAA TGATGAATCA 
TCCTGTGACA TCATGGACAT GGACCAGGGG CAGAGGCTTA ACAATGGAGC AAGCACAAAA 
GAGAACTTTG CATCTACTGA AGAGGAAAGT TCAAATGAGT CTCTACTCAT CAACAGCAGC 
AGTTCTCTAA ATCCAGAACA GACATCTCGG AAAGAGCCTT TCCTTAAAGA AGGCTGTCTA 
AATGGTGAGG CCTCCACAGA CAGTTTTGAA GGAATACCAG TTCTGGAGTG TCAGAATGGC 
AAAGCTGAAG TAGTTTCTTG CTGTGGCAGT GGAGATAAAT GTAGTTCTGA ACAAAAGACT 
CTCCTGGAGG ACCACTCAAA AGAAAAACCA GAAACTTCAA ATGAAAATCA TGGAGATGAT 
CCTGAGAAAC TAGAGGCACT GGAATGTAGC ACTAACGAGA AGTTAGAGCC TGGCCCTGAT 
GTAGAGGTTA AAGATGCAGA ACTGGATAAA GAAGGTGCTT CTAAAGTAAA GAAATATCGT 
AAGTTAATTT TAGAACAGGC AAAAACAACA AGCCTGGAAC TGGTTCCAGA AGAGCCATCT 
GAGCCTGTGC CACCTCTTAT AGTTGATCAT GAGAGATTGA AGAAATTGCT TGATTTGTTG 
GTAGATAAAA GCAACAATCT GGCAGTTGAT CAACTTGAGA GATTATATTC TCTTCTTAGT 
CAGTGCATCT ACCGTCACCG TAAAGATTAT GACAAATCAC AACTTGTAGA GAACAGAAGT 
AATCTGGGTT TCTTTGTTTA TCAAAACACA GAAATCCATG GTTGGCAGAC ACACGGAATA 
ACATGGCGCA GTTCTCCCCT CCCTTGCTTC TCGGCGACTT CACAGATGAT GCATATCACG 
TGGGCCTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117288.1
CDS	443..4474
Translation	MEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVENRSNLGFFVYQNTEIHGWQTHGITWRSSPLPCFSATSQMMHITWAC

Target ORF information:

RefSeq Version	XM_025261503.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261503.1

ORF Insert Sequence:

ATGGAAGTCA GAAAAAGCTG TCGTTCCAGG AAAAACAGAT TTGAAAGTGT GAACCAGAGT 
TTGTTATTTG ATCAACTGGT AAATAGTACT GCTGAAGCTG TACTACAGGA AATGGACAAC 
ATAAACATTA GACGGAATCG TCGATCAGGA GAAGTAGAAC GGCTTCGAAT GTGGACAGAT 
ACAGAATTTG AAAACATGGA TATGTATTCA AGAGTGAAAA GGCGAAGAAA GTCACTGAGA 
AGAAATAGTT ATGGTATACA AAATCATCAT GAAGTCTCTA CTGAGGGTGA AGAAGAAGCT 
AGAACCTCTA TATTGCTTCC TTTGGAGAAA ATCAGTATAG AATCTCAAGA GGAAGATGGA 
GATATAGAGG TTGAAGAGGC AGAAGGAGAA GAAAATGATA GGCCATATAA TTTGAGACAG 
AGAAAAACAG TGGATCGATA CCAAGCACCT CCAATAGTAC CAGCTCATCA AAAAAAGAGG 
GAAAATACCC TTTTTGATAT TCACAGATCT CCAGCAAGAA GAAGTCATAT CAGGAGAAAG 
AAGCATGCTA TTCACAGTAG TGACACAACG TCTTCTGATG AAGAACGATT TGAAAGAAGG 
AAATCAAAGA GCATGGCAAG AGCAAGAAAT AGATGTTTGC CTATGAACTT AAGAGCAGAA 
GATTTAGCTA GTGGTATTCT CCGAGAACGT GTTAAAGTGG GTGCAAGCTT GGCTGATGTT 
GATCCAATGA ATATTGATAA ATCAGTGCGG TTTGATAGCA TAGGTGGATT GAGTCATCAT 
ATTCATGCAC TAAAGGAAAT GGTAGTATTC CCACTTTTAT ATCCAGAAAT TTTTGAAAAA 
TTTAAAATTC AGCCTCCAAG AGGCTGTTTG TTTTATGGTC CTCCTGGCAC AGGTAAAACC 
TTGGTCGCCA GAGCGTTAGC TAACGAGTGC AGTCAGGGAG ACAAAAAGGT GGCTTTTTTT 
ATGCGAAAAG GAGCGGATTG TCTGAGTAAG TGGGTTGGTG AATCTGAGCG GCAGCTTAGG 
CTTCTTTTTG ATCAGGCATA TTTGATGAGA CCTTCTATAA TATTTTTTGA TGAAATAGAT 
GGATTAGCTC CAGTTCGCTC TAGCAGGCAA GATCAGATCC ACAGCTCTAT AGTGTCAACC 
CTCCTTGCTC TTATGGATGG ATTAGACAAT AGGGGCGAAA TTGTTGTTAT TGGTGCTACA 
AATAGACTTG ATTCTATAGA TCCTGCTCTT AGGAGACCTG GTCGTTTTGA CAGAGAATTT 
CTTTTCAACC TTCCAGATCA AAAGGCAAGA AAACACATTT TACAGATCCA TACCAGGGAC 
TGGAATCCAA AATTGTCAGA TGCTTTTTTA GGTGAATTGG CTGAAAAATG TGTTGGCTAT 
TGTGGAGCTG ATATCAAGGC CCTGTGCACT GAAGCTGCCC TGATTGCCCT CCGGAGGCGT 
TATCCCCAGA TCTATGCTAG CAGTCATAAA CTGCAGCTAG ATGTTTCCTC AATCGTGCTC 
AACGCCCAAG ATTTTTATCA TGCAATGCAG AATATTGTGC CTGCTTCCCA ACGTGCTGTC 
ATGTCTTCGG GACATGCACT GTCTCCCATC ATAAAACCAC TGCTGGAAAG AAGCTTCAAC 
AACATCCTAG CCGTCCTGCA AAAAGTGTTT CCTCATGCTG AGATTAGCCA GAGTGACAAG 
AAAGAAGATA TAGAAACTTT GATTTTAGAT GACAGTGAAG ATGAGAATGC TGTATCAATT 
TTCGAGACAA GTTGTCACTC AGGATCACCA AAGAAACAGT CATCAGCTGC TGCTGTACAT 
AAACCTTATC TTCATTTTAC AATGTCACCA TATCATCAGC CAACCTCTTA CAGGCCAAGG 
TTATTGCTGT CTGGAGAACG AGGCTCAGGT CAAACTTCTC ACCTTGCTCC AGCACTTTTG 
CACACTTTAG AAAAATTCTC TGTGCATAGA CTAGATCTCC CAGCACTTTA TTCAGTTAGT 
GCCAAAACAC CTGAAGAATC GTGTGCACAG ATTTTTCGTG AAGCTCGAAG AACAGTACCT 
AGTATTGTTT ACATGCCTCA CATTGGTGAT TGGTGGGAAG CTGTCAGTGA AACTGTGAGA 
GCAACTTTTC TGACTCTGCT ACAAGATATA CCGTCATTTT CACCCATATT TCTGTTGTCT 
ACCTCTGAAA CCATGTACAG TGAACTGCCT GAAGAGGTTA AATGTATCTT TAGAATACAG 
TACGAAGAGG TCTTGTATAT TCAAAGGCCT ATTGAAGAGG ACAGAAGAAA ATTTTTCCAA 
GAACTGATTC TCAATCAGGC CTCAATGGCT CCACCACGAA GGAAACACAC TGGTCTTTGT 
GCAATGGAGG TACTTCCACT TGCATTACCT TCTCCACCTC GTCAGTTATC AGAATCAGAG 
AAAATTCGGA TGGAGGACCA AGAGGAAAAT ACTTTAAGAG AGTTGCGGTT GTTTCTCAGG 
GATGTAACCA AGAGGCTGGC GACAGATAAA CGCTTTAACA TCTTCAGCAA ACCGGTGGAT 
ATTGAAGAGG TTTCAGATTA TCTTGAAGTA ATCAAGGAAC CAATGGACTT ATCAACAGTA 
ATAACTAAAA TTGATAAGCA TAATTACCTG ACTGCTAAGG ATTTCCTAAA AGATATTGAC 
CTCATCTGTA GCAATGCTTT GGAGTACAAT CCAGATAAGG ACCCAGGAGA CAAAATAATT 
AGGCACAGGG CTTGTACTCT GAAGGATACT GCACATGCCA TCATTGCAGC TGAGTTGGAT 
CCAGAATTTA ATAAACTCTG TGAAGAAATT AAGGAAGCAA GAATAAAAAG AGGCTTATCG 
GTAACAGCAG AACAAATAAA TCCTCATGGT ACTGGAGCTC GAAAGACAGA AACTAGAGTT 
GAAGAGGCAT TTCGGCACAA ACAAAGAAAT CCAATGGATG TCTGGCACAG TTCTGCAAAT 
AAATGTGCAT TTCGAGTTCG GAGAAAATCA CGGCGGCGAT CACAGTGGGG TAAAGGAATT 
ATTAAGAAAA GGAAAGTCAA TAATTTAAAA AAAGATGAAG AGGACACCAA ATTTACTGAC 
TATGAGAATC ATATGGAGGA CAGGAAATTG CTCGAAAATG GAGAGTTTGA GGTAAGCACT 
GACTGCCATG AAGAAAATGG AGAAGAGACT GGAGACTTAT CTATGACCAA TGATGAATCA 
TCCTGTGACA TCATGGACAT GGACCAGGGG CAGAGGCTTA ACAATGGAGC AAGCACAAAA 
GAGAACTTTG CATCTACTGA AGAGGAAAGT TCAAATGAGT CTCTACTCAT CAACAGCAGC 
AGTTCTCTAA ATCCAGAACA GACATCTCGG AAAGAGCCTT TCCTTAAAGA AGGCTGTCTA 
AATGGTGAGG CCTCCACAGA CAGTTTTGAA GGAATACCAG TTCTGGAGTG TCAGAATGGC 
AAAGCTGAAG TAGTTTCTTG CTGTGGCAGT GGAGATAAAT GTAGTTCTGA ACAAAAGACT 
CTCCTGGAGG ACCACTCAAA AGAAAAACCA GAAACTTCAA ATGAAAATCA TGGAGATGAT 
CCTGAGAAAC TAGAGGCACT GGAATGTAGC ACTAACGAGA AGTTAGAGCC TGGCCCTGAT 
GTAGAGGTTA AAGATGCAGA ACTGGATAAA GAAGGTGCTT CTAAAGTAAA GAAATATCGT 
AAGTTAATTT TAGAACAGGC AAAAACAACA AGCCTGGAAC TGGTTCCAGA AGAGCCATCT 
GAGCCTGTGC CACCTCTTAT AGTTGATCAT GAGAGATTGA AGAAATTGCT TGATTTGTTG 
GTAGATAAAA GCAACAATCT GGCAGTTGAT CAACTTGAGA GATTATATTC TCTTCTTAGT 
CAGTGCATCT ACCGTCACCG TAAAGATTAT GACAAATCAC AACTTGTAGA GAACAGAAGT 
AATCTGGGTT TCTTTGTTTA TCAAAACACA GAAATCCATG GTTGGCAGAC ACACGGAATA 
ACATGGCGCA GTTCTCCCCT CCCTTGCTTC TCGGCGACTT CACAGATGAT GCATATCACG 
TGGGCCTGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_025261504.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117289.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu46441
Clone ID Related Accession (Same CDS sequence)	XM_025261504.1
Accession Version	XM_025261504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACAAA GTTCAGAATC TCAAGAGGAA GATGGAGATA TAGAGGTTGA AGAGGCAGAA 
GGAGAAGAAA ATGATAGGCC ATATAATTTG AGACAGAGAA AAACAGTGGA TCGATACCAA 
GCACCTCCAA TAGTACCAGC TCATCAAAAA AAGAGGGAAA ATACCCTTTT TGATATTCAC 
AGATCTCCAG CAAGAAGAAG TCATATCAGG AGAAAGAAGC ATGCTATTCA CAGTAGTGAC 
ACAACGTCTT CTGATGAAGA ACGATTTGAA AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA 
AGAAATAGAT GTTTGCCTAT GAACTTAAGA GCAGAAGATT TAGCTAGTGG TATTCTCCGA 
GAACGTGTTA AAGTGGGTGC AAGCTTGGCT GATGTTGATC CAATGAATAT TGATAAATCA 
GTGCGGTTTG ATAGCATAGG TGGATTGAGT CATCATATTC ATGCACTAAA GGAAATGGTA 
GTATTCCCAC TTTTATATCC AGAAATTTTT GAAAAATTTA AAATTCAGCC TCCAAGAGGC 
TGTTTGTTTT ATGGTCCTCC TGGCACAGGT AAAACCTTGG TCGCCAGAGC GTTAGCTAAC 
GAGTGCAGTC AGGGAGACAA AAAGGTGGCT TTTTTTATGC GAAAAGGAGC GGATTGTCTG 
AGTAAGTGGG TTGGTGAATC TGAGCGGCAG CTTAGGCTTC TTTTTGATCA GGCATATTTG 
ATGAGACCTT CTATAATATT TTTTGATGAA ATAGATGGAT TAGCTCCAGT TCGCTCTAGC 
AGGCAAGATC AGATCCACAG CTCTATAGTG TCAACCCTCC TTGCTCTTAT GGATGGATTA 
GACAATAGGG GCGAAATTGT TGTTATTGGT GCTACAAATA GACTTGATTC TATAGATCCT 
GCTCTTAGGA GACCTGGTCG TTTTGACAGA GAATTTCTTT TCAACCTTCC AGATCAAAAG 
GCAAGAAAAC ACATTTTACA GATCCATACC AGGGACTGGA ATCCAAAATT GTCAGATGCT 
TTTTTAGGTG AATTGGCTGA AAAATGTGTT GGCTATTGTG GAGCTGATAT CAAGGCCCTG 
TGCACTGAAG CTGCCCTGAT TGCCCTCCGG AGGCGTTATC CCCAGATCTA TGCTAGCAGT 
CATAAACTGC AGCTAGATGT TTCCTCAATC GTGCTCAACG CCCAAGATTT TTATCATGCA 
ATGCAGAATA TTGTGCCTGC TTCCCAACGT GCTGTCATGT CTTCGGGACA TGCACTGTCT 
CCCATCATAA AACCACTGCT GGAAAGAAGC TTCAACAACA TCCTAGCCGT CCTGCAAAAA 
GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT GACAAGAAAG AAGATATAGA AACTTTGATT 
TTAGATGACA GTGAAGATGA GAATGCTGTA TCAATTTTCG AGACAAGTTG TCACTCAGGA 
TCACCAAAGA AACAGTCATC AGCTGCTGCT GTACATAAAC CTTATCTTCA TTTTACAATG 
TCACCATATC ATCAGCCAAC CTCTTACAGG CCAAGGTTAT TGCTGTCTGG AGAACGAGGC 
TCAGGTCAAA CTTCTCACCT TGCTCCAGCA CTTTTGCACA CTTTAGAAAA ATTCTCTGTG 
CATAGACTAG ATCTCCCAGC ACTTTATTCA GTTAGTGCCA AAACACCTGA AGAATCGTGT 
GCACAGATTT TTCGTGAAGC TCGAAGAACA GTACCTAGTA TTGTTTACAT GCCTCACATT 
GGTGATTGGT GGGAAGCTGT CAGTGAAACT GTGAGAGCAA CTTTTCTGAC TCTGCTACAA 
GATATACCGT CATTTTCACC CATATTTCTG TTGTCTACCT CTGAAACCAT GTACAGTGAA 
CTGCCTGAAG AGGTTAAATG TATCTTTAGA ATACAGTACG AAGAGGTCTT GTATATTCAA 
AGGCCTATTG AAGAGGACAG AAGAAAATTT TTCCAAGAAC TGATTCTCAA TCAGGCCTCA 
ATGGCTCCAC CACGAAGGAA ACACACTGGT CTTTGTGCAA TGGAGGTACT TCCACTTGCA 
TTACCTTCTC CACCTCGTCA GTTATCAGAA TCAGAGAAAA TTCGGATGGA GGACCAAGAG 
GAAAATACTT TAAGAGAGTT GCGGTTGTTT CTCAGGGATG TAACCAAGAG GCTGGCGACA 
GATAAACGCT TTAACATCTT CAGCAAACCG GTGGATATTG AAGAGGTTTC AGATTATCTT 
GAAGTAATCA AGGAACCAAT GGACTTATCA ACAGTAATAA CTAAAATTGA TAAGCATAAT 
TACCTGACTG CTAAGGATTT CCTAAAAGAT ATTGACCTCA TCTGTAGCAA TGCTTTGGAG 
TACAATCCAG ATAAGGACCC AGGAGACAAA ATAATTAGGC ACAGGGCTTG TACTCTGAAG 
GATACTGCAC ATGCCATCAT TGCAGCTGAG TTGGATCCAG AATTTAATAA ACTCTGTGAA 
GAAATTAAGG AAGCAAGAAT AAAAAGAGGC TTATCGGTAA CAGCAGAACA AATAAATCCT 
CATGGTACTG GAGCTCGAAA GACAGAAACT AGAGTTGAAG AGGCATTTCG GCACAAACAA 
AGAAATCCAA TGGATGTCTG GCACAGTTCT GCAAATAAAT GTGCATTTCG AGTTCGGAGA 
AAATCACGGC GGCGATCACA GTGGGGTAAA GGAATTATTA AGAAAAGGAA AGTCAATAAT 
TTAAAAAAAG ATGAAGAGGA CACCAAATTT ACTGACTATG AGAATCATAT GGAGGACAGG 
AAATTGCTCG AAAATGGAGA GTTTGAGGTA AGCACTGACT GCCATGAAGA AAATGGAGAA 
GAGACTGGAG ACTTATCTAT GACCAATGAT GAATCATCCT GTGACATCAT GGACATGGAC 
CAGGGGCAGA GGCTTAACAA TGGAGCAAGC ACAAAAGAGA ACTTTGCATC TACTGAAGAG 
GAAAGTTCAA ATGAGTCTCT ACTCATCAAC AGCAGCAGTT CTCTAAATCC AGAACAGACA 
TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC TGTCTAAATG GTGAGGCCTC CACAGACAGT 
TTTGAAGGAA TACCAGTTCT GGAGTGTCAG AATGGCAAAG CTGAAGTAGT TTCTTGCTGT 
GGCAGTGGAG ATAAATGTAG TTCTGAACAA AAGACTCTCC TGGAGGACCA CTCAAAAGAA 
AAACCAGAAA CTTCAAATGA AAATCATGGA GATGATCCTG AGAAACTAGA GGCACTGGAA 
TGTAGCACTA ACGAGAAGTT AGAGCCTGGC CCTGATGTAG AGGTTAAAGA TGCAGAACTG 
GATAAAGAAG GTGCTTCTAA AGTAAAGAAA TATCGTAAGT TAATTTTAGA ACAGGCAAAA 
ACAACAAGCC TGGAACTGGT TCCAGAAGAG CCATCTGAGC CTGTGCCACC TCTTATAGTT 
GATCATGAGA GATTGAAGAA ATTGCTTGAT TTGTTGGTAG ATAAAAGCAA CAATCTGGCA 
GTTGATCAAC TTGAGAGATT ATATTCTCTT CTTAGTCAGT GCATCTACCG TCACCGTAAA 
GATTATGACA AATCACAACT TGTAGAGAAC AGAAGTAATC TGGGTTTCTT TGTTTATCAA 
AACACAGAAA TCCATGGTTG GCAGACACAC GGAATAACAT GGCGCAGTTC TCCCCTCCCT 
TGCTTCTCGG CGACTTCACA GATGATGCAT ATCACGTGGG CCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117289.1
CDS	103..3810
Translation	MLQSSESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVENRSNLGFFVYQNTEIHGWQTHGITWRSSPLPCFSATSQMMHITWAC

Target ORF information:

RefSeq Version	XM_025261504.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261504.1

ORF Insert Sequence:

ATGCTACAAA GTTCAGAATC TCAAGAGGAA GATGGAGATA TAGAGGTTGA AGAGGCAGAA 
GGAGAAGAAA ATGATAGGCC ATATAATTTG AGACAGAGAA AAACAGTGGA TCGATACCAA 
GCACCTCCAA TAGTACCAGC TCATCAAAAA AAGAGGGAAA ATACCCTTTT TGATATTCAC 
AGATCTCCAG CAAGAAGAAG TCATATCAGG AGAAAGAAGC ATGCTATTCA CAGTAGTGAC 
ACAACGTCTT CTGATGAAGA ACGATTTGAA AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA 
AGAAATAGAT GTTTGCCTAT GAACTTAAGA GCAGAAGATT TAGCTAGTGG TATTCTCCGA 
GAACGTGTTA AAGTGGGTGC AAGCTTGGCT GATGTTGATC CAATGAATAT TGATAAATCA 
GTGCGGTTTG ATAGCATAGG TGGATTGAGT CATCATATTC ATGCACTAAA GGAAATGGTA 
GTATTCCCAC TTTTATATCC AGAAATTTTT GAAAAATTTA AAATTCAGCC TCCAAGAGGC 
TGTTTGTTTT ATGGTCCTCC TGGCACAGGT AAAACCTTGG TCGCCAGAGC GTTAGCTAAC 
GAGTGCAGTC AGGGAGACAA AAAGGTGGCT TTTTTTATGC GAAAAGGAGC GGATTGTCTG 
AGTAAGTGGG TTGGTGAATC TGAGCGGCAG CTTAGGCTTC TTTTTGATCA GGCATATTTG 
ATGAGACCTT CTATAATATT TTTTGATGAA ATAGATGGAT TAGCTCCAGT TCGCTCTAGC 
AGGCAAGATC AGATCCACAG CTCTATAGTG TCAACCCTCC TTGCTCTTAT GGATGGATTA 
GACAATAGGG GCGAAATTGT TGTTATTGGT GCTACAAATA GACTTGATTC TATAGATCCT 
GCTCTTAGGA GACCTGGTCG TTTTGACAGA GAATTTCTTT TCAACCTTCC AGATCAAAAG 
GCAAGAAAAC ACATTTTACA GATCCATACC AGGGACTGGA ATCCAAAATT GTCAGATGCT 
TTTTTAGGTG AATTGGCTGA AAAATGTGTT GGCTATTGTG GAGCTGATAT CAAGGCCCTG 
TGCACTGAAG CTGCCCTGAT TGCCCTCCGG AGGCGTTATC CCCAGATCTA TGCTAGCAGT 
CATAAACTGC AGCTAGATGT TTCCTCAATC GTGCTCAACG CCCAAGATTT TTATCATGCA 
ATGCAGAATA TTGTGCCTGC TTCCCAACGT GCTGTCATGT CTTCGGGACA TGCACTGTCT 
CCCATCATAA AACCACTGCT GGAAAGAAGC TTCAACAACA TCCTAGCCGT CCTGCAAAAA 
GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT GACAAGAAAG AAGATATAGA AACTTTGATT 
TTAGATGACA GTGAAGATGA GAATGCTGTA TCAATTTTCG AGACAAGTTG TCACTCAGGA 
TCACCAAAGA AACAGTCATC AGCTGCTGCT GTACATAAAC CTTATCTTCA TTTTACAATG 
TCACCATATC ATCAGCCAAC CTCTTACAGG CCAAGGTTAT TGCTGTCTGG AGAACGAGGC 
TCAGGTCAAA CTTCTCACCT TGCTCCAGCA CTTTTGCACA CTTTAGAAAA ATTCTCTGTG 
CATAGACTAG ATCTCCCAGC ACTTTATTCA GTTAGTGCCA AAACACCTGA AGAATCGTGT 
GCACAGATTT TTCGTGAAGC TCGAAGAACA GTACCTAGTA TTGTTTACAT GCCTCACATT 
GGTGATTGGT GGGAAGCTGT CAGTGAAACT GTGAGAGCAA CTTTTCTGAC TCTGCTACAA 
GATATACCGT CATTTTCACC CATATTTCTG TTGTCTACCT CTGAAACCAT GTACAGTGAA 
CTGCCTGAAG AGGTTAAATG TATCTTTAGA ATACAGTACG AAGAGGTCTT GTATATTCAA 
AGGCCTATTG AAGAGGACAG AAGAAAATTT TTCCAAGAAC TGATTCTCAA TCAGGCCTCA 
ATGGCTCCAC CACGAAGGAA ACACACTGGT CTTTGTGCAA TGGAGGTACT TCCACTTGCA 
TTACCTTCTC CACCTCGTCA GTTATCAGAA TCAGAGAAAA TTCGGATGGA GGACCAAGAG 
GAAAATACTT TAAGAGAGTT GCGGTTGTTT CTCAGGGATG TAACCAAGAG GCTGGCGACA 
GATAAACGCT TTAACATCTT CAGCAAACCG GTGGATATTG AAGAGGTTTC AGATTATCTT 
GAAGTAATCA AGGAACCAAT GGACTTATCA ACAGTAATAA CTAAAATTGA TAAGCATAAT 
TACCTGACTG CTAAGGATTT CCTAAAAGAT ATTGACCTCA TCTGTAGCAA TGCTTTGGAG 
TACAATCCAG ATAAGGACCC AGGAGACAAA ATAATTAGGC ACAGGGCTTG TACTCTGAAG 
GATACTGCAC ATGCCATCAT TGCAGCTGAG TTGGATCCAG AATTTAATAA ACTCTGTGAA 
GAAATTAAGG AAGCAAGAAT AAAAAGAGGC TTATCGGTAA CAGCAGAACA AATAAATCCT 
CATGGTACTG GAGCTCGAAA GACAGAAACT AGAGTTGAAG AGGCATTTCG GCACAAACAA 
AGAAATCCAA TGGATGTCTG GCACAGTTCT GCAAATAAAT GTGCATTTCG AGTTCGGAGA 
AAATCACGGC GGCGATCACA GTGGGGTAAA GGAATTATTA AGAAAAGGAA AGTCAATAAT 
TTAAAAAAAG ATGAAGAGGA CACCAAATTT ACTGACTATG AGAATCATAT GGAGGACAGG 
AAATTGCTCG AAAATGGAGA GTTTGAGGTA AGCACTGACT GCCATGAAGA AAATGGAGAA 
GAGACTGGAG ACTTATCTAT GACCAATGAT GAATCATCCT GTGACATCAT GGACATGGAC 
CAGGGGCAGA GGCTTAACAA TGGAGCAAGC ACAAAAGAGA ACTTTGCATC TACTGAAGAG 
GAAAGTTCAA ATGAGTCTCT ACTCATCAAC AGCAGCAGTT CTCTAAATCC AGAACAGACA 
TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC TGTCTAAATG GTGAGGCCTC CACAGACAGT 
TTTGAAGGAA TACCAGTTCT GGAGTGTCAG AATGGCAAAG CTGAAGTAGT TTCTTGCTGT 
GGCAGTGGAG ATAAATGTAG TTCTGAACAA AAGACTCTCC TGGAGGACCA CTCAAAAGAA 
AAACCAGAAA CTTCAAATGA AAATCATGGA GATGATCCTG AGAAACTAGA GGCACTGGAA 
TGTAGCACTA ACGAGAAGTT AGAGCCTGGC CCTGATGTAG AGGTTAAAGA TGCAGAACTG 
GATAAAGAAG GTGCTTCTAA AGTAAAGAAA TATCGTAAGT TAATTTTAGA ACAGGCAAAA 
ACAACAAGCC TGGAACTGGT TCCAGAAGAG CCATCTGAGC CTGTGCCACC TCTTATAGTT 
GATCATGAGA GATTGAAGAA ATTGCTTGAT TTGTTGGTAG ATAAAAGCAA CAATCTGGCA 
GTTGATCAAC TTGAGAGATT ATATTCTCTT CTTAGTCAGT GCATCTACCG TCACCGTAAA 
GATTATGACA AATCACAACT TGTAGAGAAC AGAAGTAATC TGGGTTTCTT TGTTTATCAA 
AACACAGAAA TCCATGGTTG GCAGACACAC GGAATAACAT GGCGCAGTTC TCCCCTCCCT 
TGCTTCTCGG CGACTTCACA GATGATGCAT ATCACGTGGG CCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_025261498.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117283.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X1, mRNA.

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CloneID	OBu46436
Clone ID Related Accession (Same CDS sequence)	XM_025261498.1
Accession Version	XM_025261498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGAAC 
AGAAGTAATC TGGGTTTCTT TGTTTATCAA AACACAGAAA TCCATGGTTG GCAGACACAC 
GGAATAACAT GGCGCAGTTC TCCCCTCCCT TGCTTCTCGG CGACTTCACA GATGATGCAT 
ATCACGTGGG CCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117283.1
CDS	320..4837
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVDIEEVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVENRSNLGFFVYQNTEIHGWQTHGITWRSSPLPCFSATSQMMHITWAC

Target ORF information:

RefSeq Version	XM_025261498.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261498.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTGGATATTG AAGAGGTTTC AGATTATCTT GAAGTAATCA AGGAACCAAT GGACTTATCA 
ACAGTAATAA CTAAAATTGA TAAGCATAAT TACCTGACTG CTAAGGATTT CCTAAAAGAT 
ATTGACCTCA TCTGTAGCAA TGCTTTGGAG TACAATCCAG ATAAGGACCC AGGAGACAAA 
ATAATTAGGC ACAGGGCTTG TACTCTGAAG GATACTGCAC ATGCCATCAT TGCAGCTGAG 
TTGGATCCAG AATTTAATAA ACTCTGTGAA GAAATTAAGG AAGCAAGAAT AAAAAGAGGC 
TTATCGGTAA CAGCAGAACA AATAAATCCT CATGGTACTG GAGCTCGAAA GACAGAAACT 
AGAGTTGAAG AGGCATTTCG GCACAAACAA AGAAATCCAA TGGATGTCTG GCACAGTTCT 
GCAAATAAAT GTGCATTTCG AGTTCGGAGA AAATCACGGC GGCGATCACA GTGGGGTAAA 
GGAATTATTA AGAAAAGGAA AGTCAATAAT TTAAAAAAAG ATGAAGAGGA CACCAAATTT 
ACTGACTATG AGAATCATAT GGAGGACAGG AAATTGCTCG AAAATGGAGA GTTTGAGGTA 
AGCACTGACT GCCATGAAGA AAATGGAGAA GAGACTGGAG ACTTATCTAT GACCAATGAT 
GAATCATCCT GTGACATCAT GGACATGGAC CAGGGGCAGA GGCTTAACAA TGGAGCAAGC 
ACAAAAGAGA ACTTTGCATC TACTGAAGAG GAAAGTTCAA ATGAGTCTCT ACTCATCAAC 
AGCAGCAGTT CTCTAAATCC AGAACAGACA TCTCGGAAAG AGCCTTTCCT TAAAGAAGGC 
TGTCTAAATG GTGAGGCCTC CACAGACAGT TTTGAAGGAA TACCAGTTCT GGAGTGTCAG 
AATGGCAAAG CTGAAGTAGT TTCTTGCTGT GGCAGTGGAG ATAAATGTAG TTCTGAACAA 
AAGACTCTCC TGGAGGACCA CTCAAAAGAA AAACCAGAAA CTTCAAATGA AAATCATGGA 
GATGATCCTG AGAAACTAGA GGCACTGGAA TGTAGCACTA ACGAGAAGTT AGAGCCTGGC 
CCTGATGTAG AGGTTAAAGA TGCAGAACTG GATAAAGAAG GTGCTTCTAA AGTAAAGAAA 
TATCGTAAGT TAATTTTAGA ACAGGCAAAA ACAACAAGCC TGGAACTGGT TCCAGAAGAG 
CCATCTGAGC CTGTGCCACC TCTTATAGTT GATCATGAGA GATTGAAGAA ATTGCTTGAT 
TTGTTGGTAG ATAAAAGCAA CAATCTGGCA GTTGATCAAC TTGAGAGATT ATATTCTCTT 
CTTAGTCAGT GCATCTACCG TCACCGTAAA GATTATGACA AATCACAACT TGTAGAGAAC 
AGAAGTAATC TGGGTTTCTT TGTTTATCAA AACACAGAAA TCCATGGTTG GCAGACACAC 
GGAATAACAT GGCGCAGTTC TCCCCTCCCT TGCTTCTCGG CGACTTCACA GATGATGCAT 
ATCACGTGGG CCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATAD2B ( XM_025261499.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117284.1 Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X2, mRNA.

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CloneID	OBu46437
Clone ID Related Accession (Same CDS sequence)	XM_025261499.1
Accession Version	XM_025261499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	ATPase family AAA domain-containing protein 2B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTTTCAGATT ATCTTGAAGT AATCAAGGAA CCAATGGACT TATCAACAGT AATAACTAAA 
ATTGATAAGC ATAATTACCT GACTGCTAAG GATTTCCTAA AAGATATTGA CCTCATCTGT 
AGCAATGCTT TGGAGTACAA TCCAGATAAG GACCCAGGAG ACAAAATAAT TAGGCACAGG 
GCTTGTACTC TGAAGGATAC TGCACATGCC ATCATTGCAG CTGAGTTGGA TCCAGAATTT 
AATAAACTCT GTGAAGAAAT TAAGGAAGCA AGAATAAAAA GAGGCTTATC GGTAACAGCA 
GAACAAATAA ATCCTCATGG TACTGGAGCT CGAAAGACAG AAACTAGAGT TGAAGAGGCA 
TTTCGGCACA AACAAAGAAA TCCAATGGAT GTCTGGCACA GTTCTGCAAA TAAATGTGCA 
TTTCGAGTTC GGAGAAAATC ACGGCGGCGA TCACAGTGGG GTAAAGGAAT TATTAAGAAA 
AGGAAAGTCA ATAATTTAAA AAAAGATGAA GAGGACACCA AATTTACTGA CTATGAGAAT 
CATATGGAGG ACAGGAAATT GCTCGAAAAT GGAGAGTTTG AGGTAAGCAC TGACTGCCAT 
GAAGAAAATG GAGAAGAGAC TGGAGACTTA TCTATGACCA ATGATGAATC ATCCTGTGAC 
ATCATGGACA TGGACCAGGG GCAGAGGCTT AACAATGGAG CAAGCACAAA AGAGAACTTT 
GCATCTACTG AAGAGGAAAG TTCAAATGAG TCTCTACTCA TCAACAGCAG CAGTTCTCTA 
AATCCAGAAC AGACATCTCG GAAAGAGCCT TTCCTTAAAG AAGGCTGTCT AAATGGTGAG 
GCCTCCACAG ACAGTTTTGA AGGAATACCA GTTCTGGAGT GTCAGAATGG CAAAGCTGAA 
GTAGTTTCTT GCTGTGGCAG TGGAGATAAA TGTAGTTCTG AACAAAAGAC TCTCCTGGAG 
GACCACTCAA AAGAAAAACC AGAAACTTCA AATGAAAATC ATGGAGATGA TCCTGAGAAA 
CTAGAGGCAC TGGAATGTAG CACTAACGAG AAGTTAGAGC CTGGCCCTGA TGTAGAGGTT 
AAAGATGCAG AACTGGATAA AGAAGGTGCT TCTAAAGTAA AGAAATATCG TAAGTTAATT 
TTAGAACAGG CAAAAACAAC AAGCCTGGAA CTGGTTCCAG AAGAGCCATC TGAGCCTGTG 
CCACCTCTTA TAGTTGATCA TGAGAGATTG AAGAAATTGC TTGATTTGTT GGTAGATAAA 
AGCAACAATC TGGCAGTTGA TCAACTTGAG AGATTATATT CTCTTCTTAG TCAGTGCATC 
TACCGTCACC GTAAAGATTA TGACAAATCA CAACTTGTAG AGAACAGAAG TAATCTGGGT 
TTCTTTGTTT ATCAAAACAC AGAAATCCAT GGTTGGCAGA CACACGGAAT AACATGGCGC 
AGTTCTCCCC TCCCTTGCTT CTCGGCGACT TCACAGATGA TGCATATCAC GTGGGCCTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117284.1
CDS	321..4823
Translation	MVNTRKSSLRLLGSKSPGPGPGPGAGVEPGATGGSSHFISSRTRSSKTRAASCPAAKAGGSGGAGVALDEARKAEADGSLSDSHVSPPAKRTLKQPDSVCKDKSKSRTTGQREEWNISSGQARLTSQPGATLPNGHSSLSLRSHPHRGEKKGDGELSCINGDMEVRKSCRSRKNRFESVNQSLLFDQLVNSTAEAVLQEMDNINIRRNRRSGEVERLRMWTDTEFENMDMYSRVKRRRKSLRRNSYGIQNHHEVSTEGEEEARTSILLPLEKISIESQEEDGDIEVEEAEGEENDRPYNLRQRKTVDRYQAPPIVPAHQKKRENTLFDIHRSPARRSHIRRKKHAIHSSDTTSSDEERFERRKSKSMARARNRCLPMNLRAEDLASGILRERVKVGASLADVDPMNIDKSVRFDSIGGLSHHIHALKEMVVFPLLYPEIFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDNRGEIVVIGATNRLDSIDPALRRPGRFDREFLFNLPDQKARKHILQIHTRDWNPKLSDAFLGELAEKCVGYCGADIKALCTEAALIALRRRYPQIYASSHKLQLDVSSIVLNAQDFYHAMQNIVPASQRAVMSSGHALSPIIKPLLERSFNNILAVLQKVFPHAEISQSDKKEDIETLILDDSEDENAVSIFETSCHSGSPKKQSSAAAVHKPYLHFTMSPYHQPTSYRPRLLLSGERGSGQTSHLAPALLHTLEKFSVHRLDLPALYSVSAKTPEESCAQIFREARRTVPSIVYMPHIGDWWEAVSETVRATFLTLLQDIPSFSPIFLLSTSETMYSELPEEVKCIFRIQYEEVLYIQRPIEEDRRKFFQELILNQASMAPPRRKHTGLCAMEVLPLALPSPPRQLSESEKIRMEDQEENTLRELRLFLRDVTKRLATDKRFNIFSKPVSDYLEVIKEPMDLSTVITKIDKHNYLTAKDFLKDIDLICSNALEYNPDKDPGDKIIRHRACTLKDTAHAIIAAELDPEFNKLCEEIKEARIKRGLSVTAEQINPHGTGARKTETRVEEAFRHKQRNPMDVWHSSANKCAFRVRRKSRRRSQWGKGIIKKRKVNNLKKDEEDTKFTDYENHMEDRKLLENGEFEVSTDCHEENGEETGDLSMTNDESSCDIMDMDQGQRLNNGASTKENFASTEEESSNESLLINSSSSLNPEQTSRKEPFLKEGCLNGEASTDSFEGIPVLECQNGKAEVVSCCGSGDKCSSEQKTLLEDHSKEKPETSNENHGDDPEKLEALECSTNEKLEPGPDVEVKDAELDKEGASKVKKYRKLILEQAKTTSLELVPEEPSEPVPPLIVDHERLKKLLDLLVDKSNNLAVDQLERLYSLLSQCIYRHRKDYDKSQLVENRSNLGFFVYQNTEIHGWQTHGITWRSSPLPCFSATSQMMHITWAC

Target ORF information:

RefSeq Version	XM_025261499.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis ATPase family, AAA domain containing 2B (ATAD2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261499.1

ORF Insert Sequence:

ATGGTGAACA CCCGGAAGAG CTCTCTGCGC CTTCTCGGAT CTAAGTCGCC TGGGCCTGGG 
CCTGGGCCTG GGGCCGGAGT AGAGCCCGGG GCGACCGGAG GCAGCAGCCA TTTCATCTCC 
TCTCGGACCC GCTCCTCCAA GACCCGTGCT GCCAGCTGCC CCGCCGCCAA GGCCGGGGGA 
AGCGGCGGCG CCGGCGTCGC TCTGGATGAG GCCCGGAAAG CTGAAGCTGA TGGTAGTTTA 
AGTGATAGCC ATGTGTCTCC TCCAGCCAAA CGCACTTTGA AACAACCTGA CTCTGTTTGC 
AAAGACAAAT CAAAATCACG AACTACTGGT CAGCGAGAAG AATGGAACAT ATCAAGTGGA 
CAGGCCAGGT TAACTTCTCA GCCTGGTGCT ACATTACCAA ACGGACATAG TAGCTTATCC 
CTTCGAAGCC ATCCCCATCG AGGGGAAAAG AAAGGAGATG GGGAACTTTC TTGTATAAAT 
GGTGACATGG AAGTCAGAAA AAGCTGTCGT TCCAGGAAAA ACAGATTTGA AAGTGTGAAC 
CAGAGTTTGT TATTTGATCA ACTGGTAAAT AGTACTGCTG AAGCTGTACT ACAGGAAATG 
GACAACATAA ACATTAGACG GAATCGTCGA TCAGGAGAAG TAGAACGGCT TCGAATGTGG 
ACAGATACAG AATTTGAAAA CATGGATATG TATTCAAGAG TGAAAAGGCG AAGAAAGTCA 
CTGAGAAGAA ATAGTTATGG TATACAAAAT CATCATGAAG TCTCTACTGA GGGTGAAGAA 
GAAGCTAGAA CCTCTATATT GCTTCCTTTG GAGAAAATCA GTATAGAATC TCAAGAGGAA 
GATGGAGATA TAGAGGTTGA AGAGGCAGAA GGAGAAGAAA ATGATAGGCC ATATAATTTG 
AGACAGAGAA AAACAGTGGA TCGATACCAA GCACCTCCAA TAGTACCAGC TCATCAAAAA 
AAGAGGGAAA ATACCCTTTT TGATATTCAC AGATCTCCAG CAAGAAGAAG TCATATCAGG 
AGAAAGAAGC ATGCTATTCA CAGTAGTGAC ACAACGTCTT CTGATGAAGA ACGATTTGAA 
AGAAGGAAAT CAAAGAGCAT GGCAAGAGCA AGAAATAGAT GTTTGCCTAT GAACTTAAGA 
GCAGAAGATT TAGCTAGTGG TATTCTCCGA GAACGTGTTA AAGTGGGTGC AAGCTTGGCT 
GATGTTGATC CAATGAATAT TGATAAATCA GTGCGGTTTG ATAGCATAGG TGGATTGAGT 
CATCATATTC ATGCACTAAA GGAAATGGTA GTATTCCCAC TTTTATATCC AGAAATTTTT 
GAAAAATTTA AAATTCAGCC TCCAAGAGGC TGTTTGTTTT ATGGTCCTCC TGGCACAGGT 
AAAACCTTGG TCGCCAGAGC GTTAGCTAAC GAGTGCAGTC AGGGAGACAA AAAGGTGGCT 
TTTTTTATGC GAAAAGGAGC GGATTGTCTG AGTAAGTGGG TTGGTGAATC TGAGCGGCAG 
CTTAGGCTTC TTTTTGATCA GGCATATTTG ATGAGACCTT CTATAATATT TTTTGATGAA 
ATAGATGGAT TAGCTCCAGT TCGCTCTAGC AGGCAAGATC AGATCCACAG CTCTATAGTG 
TCAACCCTCC TTGCTCTTAT GGATGGATTA GACAATAGGG GCGAAATTGT TGTTATTGGT 
GCTACAAATA GACTTGATTC TATAGATCCT GCTCTTAGGA GACCTGGTCG TTTTGACAGA 
GAATTTCTTT TCAACCTTCC AGATCAAAAG GCAAGAAAAC ACATTTTACA GATCCATACC 
AGGGACTGGA ATCCAAAATT GTCAGATGCT TTTTTAGGTG AATTGGCTGA AAAATGTGTT 
GGCTATTGTG GAGCTGATAT CAAGGCCCTG TGCACTGAAG CTGCCCTGAT TGCCCTCCGG 
AGGCGTTATC CCCAGATCTA TGCTAGCAGT CATAAACTGC AGCTAGATGT TTCCTCAATC 
GTGCTCAACG CCCAAGATTT TTATCATGCA ATGCAGAATA TTGTGCCTGC TTCCCAACGT 
GCTGTCATGT CTTCGGGACA TGCACTGTCT CCCATCATAA AACCACTGCT GGAAAGAAGC 
TTCAACAACA TCCTAGCCGT CCTGCAAAAA GTGTTTCCTC ATGCTGAGAT TAGCCAGAGT 
GACAAGAAAG AAGATATAGA AACTTTGATT TTAGATGACA GTGAAGATGA GAATGCTGTA 
TCAATTTTCG AGACAAGTTG TCACTCAGGA TCACCAAAGA AACAGTCATC AGCTGCTGCT 
GTACATAAAC CTTATCTTCA TTTTACAATG TCACCATATC ATCAGCCAAC CTCTTACAGG 
CCAAGGTTAT TGCTGTCTGG AGAACGAGGC TCAGGTCAAA CTTCTCACCT TGCTCCAGCA 
CTTTTGCACA CTTTAGAAAA ATTCTCTGTG CATAGACTAG ATCTCCCAGC ACTTTATTCA 
GTTAGTGCCA AAACACCTGA AGAATCGTGT GCACAGATTT TTCGTGAAGC TCGAAGAACA 
GTACCTAGTA TTGTTTACAT GCCTCACATT GGTGATTGGT GGGAAGCTGT CAGTGAAACT 
GTGAGAGCAA CTTTTCTGAC TCTGCTACAA GATATACCGT CATTTTCACC CATATTTCTG 
TTGTCTACCT CTGAAACCAT GTACAGTGAA CTGCCTGAAG AGGTTAAATG TATCTTTAGA 
ATACAGTACG AAGAGGTCTT GTATATTCAA AGGCCTATTG AAGAGGACAG AAGAAAATTT 
TTCCAAGAAC TGATTCTCAA TCAGGCCTCA ATGGCTCCAC CACGAAGGAA ACACACTGGT 
CTTTGTGCAA TGGAGGTACT TCCACTTGCA TTACCTTCTC CACCTCGTCA GTTATCAGAA 
TCAGAGAAAA TTCGGATGGA GGACCAAGAG GAAAATACTT TAAGAGAGTT GCGGTTGTTT 
CTCAGGGATG TAACCAAGAG GCTGGCGACA GATAAACGCT TTAACATCTT CAGCAAACCG 
GTTTCAGATT ATCTTGAAGT AATCAAGGAA CCAATGGACT TATCAACAGT AATAACTAAA 
ATTGATAAGC ATAATTACCT GACTGCTAAG GATTTCCTAA AAGATATTGA CCTCATCTGT 
AGCAATGCTT TGGAGTACAA TCCAGATAAG GACCCAGGAG ACAAAATAAT TAGGCACAGG 
GCTTGTACTC TGAAGGATAC TGCACATGCC ATCATTGCAG CTGAGTTGGA TCCAGAATTT 
AATAAACTCT GTGAAGAAAT TAAGGAAGCA AGAATAAAAA GAGGCTTATC GGTAACAGCA 
GAACAAATAA ATCCTCATGG TACTGGAGCT CGAAAGACAG AAACTAGAGT TGAAGAGGCA 
TTTCGGCACA AACAAAGAAA TCCAATGGAT GTCTGGCACA GTTCTGCAAA TAAATGTGCA 
TTTCGAGTTC GGAGAAAATC ACGGCGGCGA TCACAGTGGG GTAAAGGAAT TATTAAGAAA 
AGGAAAGTCA ATAATTTAAA AAAAGATGAA GAGGACACCA AATTTACTGA CTATGAGAAT 
CATATGGAGG ACAGGAAATT GCTCGAAAAT GGAGAGTTTG AGGTAAGCAC TGACTGCCAT 
GAAGAAAATG GAGAAGAGAC TGGAGACTTA TCTATGACCA ATGATGAATC ATCCTGTGAC 
ATCATGGACA TGGACCAGGG GCAGAGGCTT AACAATGGAG CAAGCACAAA AGAGAACTTT 
GCATCTACTG AAGAGGAAAG TTCAAATGAG TCTCTACTCA TCAACAGCAG CAGTTCTCTA 
AATCCAGAAC AGACATCTCG GAAAGAGCCT TTCCTTAAAG AAGGCTGTCT AAATGGTGAG 
GCCTCCACAG ACAGTTTTGA AGGAATACCA GTTCTGGAGT GTCAGAATGG CAAAGCTGAA 
GTAGTTTCTT GCTGTGGCAG TGGAGATAAA TGTAGTTCTG AACAAAAGAC TCTCCTGGAG 
GACCACTCAA AAGAAAAACC AGAAACTTCA AATGAAAATC ATGGAGATGA TCCTGAGAAA 
CTAGAGGCAC TGGAATGTAG CACTAACGAG AAGTTAGAGC CTGGCCCTGA TGTAGAGGTT 
AAAGATGCAG AACTGGATAA AGAAGGTGCT TCTAAAGTAA AGAAATATCG TAAGTTAATT 
TTAGAACAGG CAAAAACAAC AAGCCTGGAA CTGGTTCCAG AAGAGCCATC TGAGCCTGTG 
CCACCTCTTA TAGTTGATCA TGAGAGATTG AAGAAATTGC TTGATTTGTT GGTAGATAAA 
AGCAACAATC TGGCAGTTGA TCAACTTGAG AGATTATATT CTCTTCTTAG TCAGTGCATC 
TACCGTCACC GTAAAGATTA TGACAAATCA CAACTTGTAG AGAACAGAAG TAATCTGGGT 
TTCTTTGTTT ATCAAAACAC AGAAATCCAT GGTTGGCAGA CACACGGAAT AACATGGCGC 
AGTTCTCCCC TCCCTTGCTT CTCGGCGACT TCACAGATGA TGCATATCAC GTGGGCCTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ATAD2B
Entrez Gene ID	102391831
Full Name	ATPase family, AAA domain containing 2B
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ATAD2B cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

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