OPLAH cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	OPLAH
Entrez Gene ID	102391226
Full Name	5-oxoprolinase, ATP-hydrolysing
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025265602.1	XP_025121387.1	5-oxoprolinase isoform X4
XM_006041072.1	XP_006041134.1	5-oxoprolinase isoform X2
XM_025265599.1	XP_025121384.1	5-oxoprolinase isoform X1
XM_025265601.1	XP_025121386.1	5-oxoprolinase isoform X3
XM_006041071.1	XP_006041133.1	5-oxoprolinase isoform X1
XM_025265600.1	XP_025121385.1	5-oxoprolinase isoform X2

Pathways from BioSystems
Gene Ontology

The following OPLAH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OPLAH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu00883	XM_006041072.1 Latest version!	Bubalus bubalis 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu49696	XM_025265599.1 Latest version!	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu49697	XM_025265600.1 Latest version!	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBu49698	XM_025265601.1 Latest version!	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu49699	XM_025265602.1 Latest version!	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu00882	XM_006041071.1 Latest version!	Bubalus bubalis 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OPLAH ( XM_006041072.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006041134.1 Bubalus bubalis 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu00883
Clone ID Related Accession (Same CDS sequence)	XM_006041072.1
Accession Version	XM_006041072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	5-oxoprolinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005781533.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT 
GCCAACTATG CGGATGCCCC CACTGAGGGC ATCCGCCGTG TCCTGGAGCA GGAGGGGGGC 
GTGCTGCTGC CGCGGGACCG GCCACTGGAC ACAAGTCGCA TCGCCAGCAT CCGCATGGGC 
ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGAGGGGAG AGCGGGTGGC ACTGCTGGTG 
ACGCGTGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCTCGGA CCTCTTTGAC 
CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG 
GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCTGTGA AAGGCTGCAC AGGGGACCTA 
CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGGCTCCTG 
TCCCGGGGCA TCCGCAGCTT GGCTGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC 
GAGCAGCAGG TGGGAGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG 
GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC CGACGCCTAC 
CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGCC GTGGCTTCCA GGGTCAGCTC 
AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC 
AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCTGGGGGCG TGGTTGGTTA CTCAGCCACC 
ACCTACCGAG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC 
GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC 
ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACGGTGGCAG CGGGTGGGGG CTCCCGCCTC 
TTCTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCCGGCCCT 
GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG 
GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACAGAGG TCAACAGCTT CCTGACCAAT 
GGGCCTTACC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG 
GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC 
TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC 
CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG 
GGGCTGGCCC TGGCCGACGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATACT 
CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCACC TGGAGGAGCA GTGTGTGGAG 
GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG 
CGCTACCAGG GCACGGACTG CGCCTTGATG GTGTCTGCCC ACCAGCACCC AGCCAGTGCC 
CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC 
GGCTTCATTA TTCCCGAGCG GCAGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC 
AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT 
AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA 
GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC 
ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT 
GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACACAGCTTG ACCCCATCCA CCTTTCCATC 
TTCTCCCACC GCTTCATGAG CATCGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC 
ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG 
GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG 
CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGACGTGCT GCTGAGCAAC 
CACCCCAGCG CGGGGGGCAG CCACCTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG 
CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG 
GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC 
TTCCTGTCCT TTAAACTTGT GCAGGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC 
CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG 
TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC 
ATCGGGCAGT ACGGCCTGGA CGTGGTACAG GCCTACATGG GCCACATTCA GGCAAACGCG 
GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGCT CCCCTATCCG ACTCCGAGTG 
CAGATCAACA TGAGTCAGCG GCTCAGGGCC AGAGGTGTTC GGCAACCTCA ACGCGCCGCG 
GGCCATCACG CTGTCTGCGC TCATCTACTG TCTTCGCTGT CTGGTCGGCC GCGACATTCC 
ACTCAACCAG GGCTGCCTGG CTCCGGTGCG CGTGGTGATT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006041134.1
CDS	102..3266
Translation	MGGPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRVLEQEGGVLLPRDRPLDTSRIASIRMGTTVATNALLERRGERVALLVTRGFRDLLHVGTQARSDLFDLAVPMPETLYEEVLEVDERVVLYRGEPGAGTPVKGCTGDLLEVQQPVDLGGLRGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGYSATTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVATEVNSFLTNGPYPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLPYTPETFAQLDQRLGHLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPASARSPRAGDFGAAFVERYMREFGFIIPERQVVVDDVRVRGTGSSSLRLEDVPKAHSGPPRVDKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDIRISVGAETASVVGTQLDPIHLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKIPGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQRLRARGVRQPQRAAGHHAVCAHLLSSLSGRPRHSTQPGLPGSGARGDS

Target ORF information:

RefSeq Version	XM_006041072.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006041072.1

ORF Insert Sequence:

ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT 
GCCAACTATG CGGATGCCCC CACTGAGGGC ATCCGCCGTG TCCTGGAGCA GGAGGGGGGC 
GTGCTGCTGC CGCGGGACCG GCCACTGGAC ACAAGTCGCA TCGCCAGCAT CCGCATGGGC 
ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGAGGGGAG AGCGGGTGGC ACTGCTGGTG 
ACGCGTGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCTCGGA CCTCTTTGAC 
CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG 
GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCTGTGA AAGGCTGCAC AGGGGACCTA 
CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGGCTCCTG 
TCCCGGGGCA TCCGCAGCTT GGCTGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC 
GAGCAGCAGG TGGGAGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG 
GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC CGACGCCTAC 
CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGCC GTGGCTTCCA GGGTCAGCTC 
AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC 
AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCTGGGGGCG TGGTTGGTTA CTCAGCCACC 
ACCTACCGAG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC 
GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC 
ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACGGTGGCAG CGGGTGGGGG CTCCCGCCTC 
TTCTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCCGGCCCT 
GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG 
GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACAGAGG TCAACAGCTT CCTGACCAAT 
GGGCCTTACC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG 
GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC 
TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC 
CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG 
GGGCTGGCCC TGGCCGACGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATACT 
CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCACC TGGAGGAGCA GTGTGTGGAG 
GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG 
CGCTACCAGG GCACGGACTG CGCCTTGATG GTGTCTGCCC ACCAGCACCC AGCCAGTGCC 
CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC 
GGCTTCATTA TTCCCGAGCG GCAGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC 
AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT 
AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA 
GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC 
ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT 
GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACACAGCTTG ACCCCATCCA CCTTTCCATC 
TTCTCCCACC GCTTCATGAG CATCGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC 
ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG 
GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG 
CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGACGTGCT GCTGAGCAAC 
CACCCCAGCG CGGGGGGCAG CCACCTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG 
CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG 
GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC 
TTCCTGTCCT TTAAACTTGT GCAGGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC 
CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG 
TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC 
ATCGGGCAGT ACGGCCTGGA CGTGGTACAG GCCTACATGG GCCACATTCA GGCAAACGCG 
GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGCT CCCCTATCCG ACTCCGAGTG 
CAGATCAACA TGAGTCAGCG GCTCAGGGCC AGAGGTGTTC GGCAACCTCA ACGCGCCGCG 
GGCCATCACG CTGTCTGCGC TCATCTACTG TCTTCGCTGT CTGGTCGGCC GCGACATTCC 
ACTCAACCAG GGCTGCCTGG CTCCGGTGCG CGTGGTGATT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPLAH ( XM_025265599.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025121384.1 Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu49696
Clone ID Related Accession (Same CDS sequence)	XM_025265599.1
Accession Version	XM_025265599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	5-oxoprolinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTCC TCAGCAGCTA CGAGGGCCTG CGGCAGGAGA TCCAGCGGCT GGCACAGGAG 
AACGAGGAGC TGAGGCGGCT GGTGCAGCTC ATCCAGGAGA ACCAGGAGCT GAAGCTGGTG 
CTCAGGAACC GGGGCAGCAG CCTGAGCTTC TGTGGCCCCG GGCTCCTGGC TGAGGTGGCT 
GCCAGTCCCC GGCTCCCCCG GCGCAGAACC ATCAAGTTCA AGGATGTGGA GAGAGTTCTC 
CCAGGACTGC CGGCTGAGGA GGCGCTGCTG GACTCCAGCC CCATTACCAT GGGTGGCCCG 
GAGGGGCGCT TCCATTTTGC CATCGACCGT GGAGGCACCT TCACAGATGT CTTTGCCCAG 
TGCCCAGGGG GGCACGTGAG GGTCCTAAAG CTGCTTTCAG AAGACCCTGC CAACTATGCG 
GATGCCCCCA CTGAGGGCAT CCGCCGTGTC CTGGAGCAGG AGGGGGGCGT GCTGCTGCCG 
CGGGACCGGC CACTGGACAC AAGTCGCATC GCCAGCATCC GCATGGGCAC CACGGTGGCG 
ACCAATGCGC TGTTGGAACG GAGGGGAGAG CGGGTGGCAC TGCTGGTGAC GCGTGGGTTC 
CGAGACCTGC TGCACGTGGG CACCCAGGCC CGCTCGGACC TCTTTGACCT GGCCGTGCCC 
ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
GGGGAGCCGG GTGCTGGGAC ACCTGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG 
CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GGCTCCTGTC CCGGGGCATC 
CGCAGCTTGG CTGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG 
GGAGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC 
ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCCG ACGCCTACCT CACGCCCACC 
ATCCAGCGCT ACGTGCAGGG CTTCCGCCGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG 
GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC 
GCTGTGCTCT CCGGCCCTGC TGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGAGTG 
GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC 
TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC 
CCCCAGCTGG ATATCAACAC GGTGGCAGCG GGTGGGGGCT CCCGCCTCTT CTTCAGGTCG 
GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CCGGCCCTGC CTGCTACCGA 
AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA 
AAGGCCCTGG AGGCTGTGGC CACAGAGGTC AACAGCTTCC TGACCAATGG GCCTTACCCG 
GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC 
ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG 
CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC 
ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG 
GCCGACGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATACTCC CGAGACCTTT 
GCGCAGCTGG ACCAGAGGCT GGGCCACCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC 
CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC 
ACGGACTGCG CCTTGATGGT GTCTGCCCAC CAGCACCCAG CCAGTGCCCG CTCACCCCGA 
GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT 
CCCGAGCGGC AGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT 
CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG 
TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT 
GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG 
CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG 
ACAGCCAGCG TGGTGGGCAC ACAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC 
TTCATGAGCA TCGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC 
ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC 
AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT 
CAGCAGTTGG GGGCTGATCT CCACCCCGGC GACGTGCTGC TGAGCAACCA CCCCAGCGCG 
GGGGGCAGCC ACCTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA 
CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA 
GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTGTCCTTT 
AAACTTGTGC AGGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA 
GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC 
GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC 
GGCCTGGACG TGGTACAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG 
AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ACCACATGGA CGACGGCTCC CCTATCCGAC TCCGAGTGCA GATCAACATG 
AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC 
AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTCGGC 
CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC 
TCCATCCTAG ACCCTTCCCC CGATGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG 
CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CAGCTTCCCA GGGCTGCATG 
AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGCGCG 
GGCGCGGGCC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC 
ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA 
AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ACGGCATTAT CCGTGAGCTG 
CTTTTCCGCG AGGAGGCGCT GCTTTCCGTG CTGACCGAGC GCCGCGCCTT CCAGCCGTAT 
GGCCTGATGG GGGGTGAGCC TGGTGCCCGA GGCCTAAACC TACTCATCCG GAAGGACGGC 
CGGACAGTGA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCTGGGGA CGTGTTCTGT 
CTCCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAGG ACCCCGCCCC ACTGCCAGGT 
TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG CGGGGCAGCG TGTATGAGTA CCGCAGGGCC 
CAGGAGGCTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025121384.1
CDS	34..4188
Translation	MSLLSSYEGLRQEIQRLAQENEELRRLVQLIQENQELKLVLRNRGSSLSFCGPGLLAEVAASPRLPRRRTIKFKDVERVLPGLPAEEALLDSSPITMGGPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRVLEQEGGVLLPRDRPLDTSRIASIRMGTTVATNALLERRGERVALLVTRGFRDLLHVGTQARSDLFDLAVPMPETLYEEVLEVDERVVLYRGEPGAGTPVKGCTGDLLEVQQPVDLGGLRGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGYSATTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVATEVNSFLTNGPYPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLPYTPETFAQLDQRLGHLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPASARSPRAGDFGAAFVERYMREFGFIIPERQVVVDDVRVRGTGSSSLRLEDVPKAHSGPPRVDKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDIRISVGAETASVVGTQLDPIHLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKIPGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQGSAVFDFSGSGPEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPKGSILDPSPDAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRLGSGGRGRFRGGDGIIRELLFREEALLSVLTERRAFQPYGLMGGEPGARGLNLLIRKDGRTVNLGGKTSVPVYPGDVFCLHTPGGGGYGDPEDPAPLPGSPLQPLAFPERGSVYEYRRAQEAV

Target ORF information:

RefSeq Version	XM_025265599.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025265599.1

ORF Insert Sequence:

ATGTCGCTCC TCAGCAGCTA CGAGGGCCTG CGGCAGGAGA TCCAGCGGCT GGCACAGGAG 
AACGAGGAGC TGAGGCGGCT GGTGCAGCTC ATCCAGGAGA ACCAGGAGCT GAAGCTGGTG 
CTCAGGAACC GGGGCAGCAG CCTGAGCTTC TGTGGCCCCG GGCTCCTGGC TGAGGTGGCT 
GCCAGTCCCC GGCTCCCCCG GCGCAGAACC ATCAAGTTCA AGGATGTGGA GAGAGTTCTC 
CCAGGACTGC CGGCTGAGGA GGCGCTGCTG GACTCCAGCC CCATTACCAT GGGTGGCCCG 
GAGGGGCGCT TCCATTTTGC CATCGACCGT GGAGGCACCT TCACAGATGT CTTTGCCCAG 
TGCCCAGGGG GGCACGTGAG GGTCCTAAAG CTGCTTTCAG AAGACCCTGC CAACTATGCG 
GATGCCCCCA CTGAGGGCAT CCGCCGTGTC CTGGAGCAGG AGGGGGGCGT GCTGCTGCCG 
CGGGACCGGC CACTGGACAC AAGTCGCATC GCCAGCATCC GCATGGGCAC CACGGTGGCG 
ACCAATGCGC TGTTGGAACG GAGGGGAGAG CGGGTGGCAC TGCTGGTGAC GCGTGGGTTC 
CGAGACCTGC TGCACGTGGG CACCCAGGCC CGCTCGGACC TCTTTGACCT GGCCGTGCCC 
ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
GGGGAGCCGG GTGCTGGGAC ACCTGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG 
CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GGCTCCTGTC CCGGGGCATC 
CGCAGCTTGG CTGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG 
GGAGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC 
ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCCG ACGCCTACCT CACGCCCACC 
ATCCAGCGCT ACGTGCAGGG CTTCCGCCGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG 
GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC 
GCTGTGCTCT CCGGCCCTGC TGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGAGTG 
GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC 
TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC 
CCCCAGCTGG ATATCAACAC GGTGGCAGCG GGTGGGGGCT CCCGCCTCTT CTTCAGGTCG 
GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CCGGCCCTGC CTGCTACCGA 
AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA 
AAGGCCCTGG AGGCTGTGGC CACAGAGGTC AACAGCTTCC TGACCAATGG GCCTTACCCG 
GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC 
ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG 
CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC 
ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG 
GCCGACGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATACTCC CGAGACCTTT 
GCGCAGCTGG ACCAGAGGCT GGGCCACCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC 
CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC 
ACGGACTGCG CCTTGATGGT GTCTGCCCAC CAGCACCCAG CCAGTGCCCG CTCACCCCGA 
GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT 
CCCGAGCGGC AGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT 
CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG 
TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT 
GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG 
CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG 
ACAGCCAGCG TGGTGGGCAC ACAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC 
TTCATGAGCA TCGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC 
ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC 
AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT 
CAGCAGTTGG GGGCTGATCT CCACCCCGGC GACGTGCTGC TGAGCAACCA CCCCAGCGCG 
GGGGGCAGCC ACCTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA 
CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA 
GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTGTCCTTT 
AAACTTGTGC AGGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA 
GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC 
GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC 
GGCCTGGACG TGGTACAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG 
AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ACCACATGGA CGACGGCTCC CCTATCCGAC TCCGAGTGCA GATCAACATG 
AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC 
AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTCGGC 
CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC 
TCCATCCTAG ACCCTTCCCC CGATGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG 
CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CAGCTTCCCA GGGCTGCATG 
AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGCGCG 
GGCGCGGGCC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC 
ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA 
AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ACGGCATTAT CCGTGAGCTG 
CTTTTCCGCG AGGAGGCGCT GCTTTCCGTG CTGACCGAGC GCCGCGCCTT CCAGCCGTAT 
GGCCTGATGG GGGGTGAGCC TGGTGCCCGA GGCCTAAACC TACTCATCCG GAAGGACGGC 
CGGACAGTGA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCTGGGGA CGTGTTCTGT 
CTCCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAGG ACCCCGCCCC ACTGCCAGGT 
TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG CGGGGCAGCG TGTATGAGTA CCGCAGGGCC 
CAGGAGGCTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPLAH ( XM_025265600.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025121385.1 Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X2, mRNA.

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CloneID	OBu49697
Clone ID Related Accession (Same CDS sequence)	XM_025265600.1
Accession Version	XM_025265600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	5-oxoprolinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTCC TCAGCAGCTA CGAGGGCCTG CGGCAGGAGA TCCAGCGGCT GGCACAGGAG 
AACGAGGAGC TGAGGCGGCT GGTGCAGCTC ATCCAGGAGA ACCAGGAGCT GAAGCTGGTG 
CTCAGGAACC GGGGCAGCAG CCTGAGCTTC TGTGGCCCCG GGCTCCTGGC TGAGGTGGCT 
GCCAGTCCCC GGCTCCCCCG GCGCAGAACC ATCAAGTTCA AGGATGTGGA GAGAGTTCTC 
CCAGGACTGC CGGCTGAGGA GGCGCTGCTG GACTCCAGCC CCATTACCAT GGGTGGCCCG 
GAGGGGCGCT TCCATTTTGC CATCGACCGT GGAGGCACCT TCACAGATGT CTTTGCCCAG 
TGCCCAGGGG GGCACGTGAG GGTCCTAAAG CTGCTTTCAG AAGACCCTGC CAACTATGCG 
GATGCCCCCA CTGAGGGCAT CCGCCGTGTC CTGGAGCAGG AGGGGGGCGT GCTGCTGCCG 
CGGGACCGGC CACTGGACAC AAGTCGCATC GCCAGCATCC GCATGGGCAC CACGGTGGCG 
ACCAATGCGC TGTTGGAACG GAGGGGAGAG CGGGTGGCAC TGCTGGTGAC GCGTGGGTTC 
CGAGACCTGC TGCACGTGGG CACCCAGGCC CGCTCGGACC TCTTTGACCT GGCCGTGCCC 
ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
GGGGAGCCGG GTGCTGGGAC ACCTGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG 
CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GGCTCCTGTC CCGGGGCATC 
CGCAGCTTGG CTGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG 
GGAGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC 
ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCCG ACGCCTACCT CACGCCCACC 
ATCCAGCGCT ACGTGCAGGG CTTCCGCCGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG 
GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC 
GCTGTGCTCT CCGGCCCTGC TGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGAGTG 
GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC 
TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC 
CCCCAGCTGG ATATCAACAC GGTGGCAGCG GGTGGGGGCT CCCGCCTCTT CTTCAGGTCG 
GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CCGGCCCTGC CTGCTACCGA 
AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA 
AAGGCCCTGG AGGCTGTGGC CACAGAGGTC AACAGCTTCC TGACCAATGG GCCTTACCCG 
GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC 
ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG 
CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC 
ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG 
GCCGACGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATACTCC CGAGACCTTT 
GCGCAGCTGG ACCAGAGGCT GGGCCACCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC 
CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC 
ACGGACTGCG CCTTGATGGT GTCTGCCCAC CAGCACCCAG CCAGTGCCCG CTCACCCCGA 
GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT 
CCCGAGCGGC AGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT 
CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG 
TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT 
GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG 
CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG 
ACAGCCAGCG TGGTGGGCAC ACAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC 
TTCATGAGCA TCGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC 
ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC 
AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT 
CAGCAGTTGG GGGCTGATCT CCACCCCGGC GACGTGCTGC TGAGCAACCA CCCCAGCGCG 
GGGGGCAGCC ACCTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA 
CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA 
GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTGTCCTTT 
AAACTTGTGC AGGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA 
GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC 
GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC 
GGCCTGGACG TGGTACAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG 
AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ACCACATGGA CGACGGCTCC CCTATCCGAC TCCGAGTGCA GATCAACATG 
AGTCAGGGCT GCCTGGCTCC GGTGCGCGTG GTGATTCCTA AAGGCTCCAT CCTAGACCCT 
TCCCCCGATG CGGCCGTGGT GGGCGGCAAC GTGCTCACGT CGCAGCGCGT GGTGGACGTC 
ATCCTGGGGG CCTTCGGGGC CTGCGCAGCT TCCCAGGGCT GCATGAACAA CGTGACCTTG 
GGCAACGCCC ACATGGGCTA CTACGAGACG GTGGCGGGCG GCGCGGGCGC GGGCCCGGGT 
TGGCACGGGC GCAGCGGCGT GCACAGCCAC ATGACCAACA CGCGCATCAC CGACCCCGAG 
ATCCTGGAGA GCAGGTACCC GGTTATCCTG CGCCGCTTCG AGCTAAGGCT GGGGTCCGGG 
GGTCGCGGCC GCTTCCGGGG CGGCGACGGC ATTATCCGTG AGCTGCTTTT CCGCGAGGAG 
GCGCTGCTTT CCGTGCTGAC CGAGCGCCGC GCCTTCCAGC CGTATGGCCT GATGGGGGGT 
GAGCCTGGTG CCCGAGGCCT AAACCTACTC ATCCGGAAGG ACGGCCGGAC AGTGAACCTG 
GGTGGGAAGA CCTCGGTGCC CGTGTACCCT GGGGACGTGT TCTGTCTCCA CACACCAGGC 
GGTGGGGGCT ACGGGGACCC GGAGGACCCC GCCCCACTGC CAGGTTCGCC CTTGCAGCCC 
CTAGCCTTCC CGGAGCGGGG CAGCGTGTAT GAGTACCGCA GGGCCCAGGA GGCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025121385.1
CDS	35..4054
Translation	MSLLSSYEGLRQEIQRLAQENEELRRLVQLIQENQELKLVLRNRGSSLSFCGPGLLAEVAASPRLPRRRTIKFKDVERVLPGLPAEEALLDSSPITMGGPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRVLEQEGGVLLPRDRPLDTSRIASIRMGTTVATNALLERRGERVALLVTRGFRDLLHVGTQARSDLFDLAVPMPETLYEEVLEVDERVVLYRGEPGAGTPVKGCTGDLLEVQQPVDLGGLRGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGYSATTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVATEVNSFLTNGPYPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLPYTPETFAQLDQRLGHLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPASARSPRAGDFGAAFVERYMREFGFIIPERQVVVDDVRVRGTGSSSLRLEDVPKAHSGPPRVDKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDIRISVGAETASVVGTQLDPIHLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKIPGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQGCLAPVRVVIPKGSILDPSPDAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRLGSGGRGRFRGGDGIIRELLFREEALLSVLTERRAFQPYGLMGGEPGARGLNLLIRKDGRTVNLGGKTSVPVYPGDVFCLHTPGGGGYGDPEDPAPLPGSPLQPLAFPERGSVYEYRRAQEAV

Target ORF information:

RefSeq Version	XM_025265600.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025265600.1

ORF Insert Sequence:

ATGTCGCTCC TCAGCAGCTA CGAGGGCCTG CGGCAGGAGA TCCAGCGGCT GGCACAGGAG 
AACGAGGAGC TGAGGCGGCT GGTGCAGCTC ATCCAGGAGA ACCAGGAGCT GAAGCTGGTG 
CTCAGGAACC GGGGCAGCAG CCTGAGCTTC TGTGGCCCCG GGCTCCTGGC TGAGGTGGCT 
GCCAGTCCCC GGCTCCCCCG GCGCAGAACC ATCAAGTTCA AGGATGTGGA GAGAGTTCTC 
CCAGGACTGC CGGCTGAGGA GGCGCTGCTG GACTCCAGCC CCATTACCAT GGGTGGCCCG 
GAGGGGCGCT TCCATTTTGC CATCGACCGT GGAGGCACCT TCACAGATGT CTTTGCCCAG 
TGCCCAGGGG GGCACGTGAG GGTCCTAAAG CTGCTTTCAG AAGACCCTGC CAACTATGCG 
GATGCCCCCA CTGAGGGCAT CCGCCGTGTC CTGGAGCAGG AGGGGGGCGT GCTGCTGCCG 
CGGGACCGGC CACTGGACAC AAGTCGCATC GCCAGCATCC GCATGGGCAC CACGGTGGCG 
ACCAATGCGC TGTTGGAACG GAGGGGAGAG CGGGTGGCAC TGCTGGTGAC GCGTGGGTTC 
CGAGACCTGC TGCACGTGGG CACCCAGGCC CGCTCGGACC TCTTTGACCT GGCCGTGCCC 
ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
GGGGAGCCGG GTGCTGGGAC ACCTGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG 
CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GGCTCCTGTC CCGGGGCATC 
CGCAGCTTGG CTGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG 
GGAGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC 
ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCCG ACGCCTACCT CACGCCCACC 
ATCCAGCGCT ACGTGCAGGG CTTCCGCCGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG 
GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC 
GCTGTGCTCT CCGGCCCTGC TGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGAGTG 
GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC 
TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC 
CCCCAGCTGG ATATCAACAC GGTGGCAGCG GGTGGGGGCT CCCGCCTCTT CTTCAGGTCG 
GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CCGGCCCTGC CTGCTACCGA 
AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA 
AAGGCCCTGG AGGCTGTGGC CACAGAGGTC AACAGCTTCC TGACCAATGG GCCTTACCCG 
GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC 
ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG 
CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC 
ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG 
GCCGACGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATACTCC CGAGACCTTT 
GCGCAGCTGG ACCAGAGGCT GGGCCACCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC 
CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC 
ACGGACTGCG CCTTGATGGT GTCTGCCCAC CAGCACCCAG CCAGTGCCCG CTCACCCCGA 
GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT 
CCCGAGCGGC AGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT 
CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG 
TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT 
GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG 
CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG 
ACAGCCAGCG TGGTGGGCAC ACAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC 
TTCATGAGCA TCGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC 
ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC 
AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT 
CAGCAGTTGG GGGCTGATCT CCACCCCGGC GACGTGCTGC TGAGCAACCA CCCCAGCGCG 
GGGGGCAGCC ACCTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA 
CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA 
GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTGTCCTTT 
AAACTTGTGC AGGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA 
GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC 
GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC 
GGCCTGGACG TGGTACAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG 
AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ACCACATGGA CGACGGCTCC CCTATCCGAC TCCGAGTGCA GATCAACATG 
AGTCAGGGCT GCCTGGCTCC GGTGCGCGTG GTGATTCCTA AAGGCTCCAT CCTAGACCCT 
TCCCCCGATG CGGCCGTGGT GGGCGGCAAC GTGCTCACGT CGCAGCGCGT GGTGGACGTC 
ATCCTGGGGG CCTTCGGGGC CTGCGCAGCT TCCCAGGGCT GCATGAACAA CGTGACCTTG 
GGCAACGCCC ACATGGGCTA CTACGAGACG GTGGCGGGCG GCGCGGGCGC GGGCCCGGGT 
TGGCACGGGC GCAGCGGCGT GCACAGCCAC ATGACCAACA CGCGCATCAC CGACCCCGAG 
ATCCTGGAGA GCAGGTACCC GGTTATCCTG CGCCGCTTCG AGCTAAGGCT GGGGTCCGGG 
GGTCGCGGCC GCTTCCGGGG CGGCGACGGC ATTATCCGTG AGCTGCTTTT CCGCGAGGAG 
GCGCTGCTTT CCGTGCTGAC CGAGCGCCGC GCCTTCCAGC CGTATGGCCT GATGGGGGGT 
GAGCCTGGTG CCCGAGGCCT AAACCTACTC ATCCGGAAGG ACGGCCGGAC AGTGAACCTG 
GGTGGGAAGA CCTCGGTGCC CGTGTACCCT GGGGACGTGT TCTGTCTCCA CACACCAGGC 
GGTGGGGGCT ACGGGGACCC GGAGGACCCC GCCCCACTGC CAGGTTCGCC CTTGCAGCCC 
CTAGCCTTCC CGGAGCGGGG CAGCGTGTAT GAGTACCGCA GGGCCCAGGA GGCTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPLAH ( XM_025265601.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025121386.1 Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu49698
Clone ID Related Accession (Same CDS sequence)	XM_025265601.1
Accession Version	XM_025265601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	5-oxoprolinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT 
GCCAACTATG CGGATGCCCC CACTGAGGGC ATCCGCCGTG TCCTGGAGCA GGAGGGGGGC 
GTGCTGCTGC CGCGGGACCG GCCACTGGAC ACAAGTCGCA TCGCCAGCAT CCGCATGGGC 
ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGAGGGGAG AGCGGGTGGC ACTGCTGGTG 
ACGCGTGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCTCGGA CCTCTTTGAC 
CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG 
GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCTGTGA AAGGCTGCAC AGGGGACCTA 
CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGGCTCCTG 
TCCCGGGGCA TCCGCAGCTT GGCTGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC 
GAGCAGCAGG TGGGAGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG 
GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC CGACGCCTAC 
CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGCC GTGGCTTCCA GGGTCAGCTC 
AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC 
AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCTGGGGGCG TGGTTGGTTA CTCAGCCACC 
ACCTACCGAG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC 
GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC 
ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACGGTGGCAG CGGGTGGGGG CTCCCGCCTC 
TTCTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCCGGCCCT 
GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG 
GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACAGAGG TCAACAGCTT CCTGACCAAT 
GGGCCTTACC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG 
GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC 
TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC 
CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG 
GGGCTGGCCC TGGCCGACGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATACT 
CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCACC TGGAGGAGCA GTGTGTGGAG 
GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG 
CGCTACCAGG GCACGGACTG CGCCTTGATG GTGTCTGCCC ACCAGCACCC AGCCAGTGCC 
CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC 
GGCTTCATTA TTCCCGAGCG GCAGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC 
AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT 
AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA 
GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC 
ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT 
GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACACAGCTTG ACCCCATCCA CCTTTCCATC 
TTCTCCCACC GCTTCATGAG CATCGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC 
ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG 
GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG 
CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGACGTGCT GCTGAGCAAC 
CACCCCAGCG CGGGGGGCAG CCACCTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG 
CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG 
GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC 
TTCCTGTCCT TTAAACTTGT GCAGGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC 
CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG 
TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC 
ATCGGGCAGT ACGGCCTGGA CGTGGTACAG GCCTACATGG GCCACATTCA GGCAAACGCG 
GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGCT CCCCTATCCG ACTCCGAGTG 
CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG 
TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC 
TGTCTGGTCG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG 
ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGATGCGG CCGTGGTGGG CGGCAACGTG 
CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCAGCTTCC 
CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG 
GCGGGCGGCG CGGGCGCGGG CCCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG 
ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC 
CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGACGGCATT 
ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTTTCCG TGCTGACCGA GCGCCGCGCC 
TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GAGGCCTAAA CCTACTCATC 
CGGAAGGACG GCCGGACAGT GAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCTGGG 
GACGTGTTCT GTCTCCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA GGACCCCGCC 
CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGCGGGGCAG CGTGTATGAG 
TACCGCAGGG CCCAGGAGGC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025121386.1
CDS	206..4072
Translation	MGGPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRVLEQEGGVLLPRDRPLDTSRIASIRMGTTVATNALLERRGERVALLVTRGFRDLLHVGTQARSDLFDLAVPMPETLYEEVLEVDERVVLYRGEPGAGTPVKGCTGDLLEVQQPVDLGGLRGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGYSATTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVATEVNSFLTNGPYPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLPYTPETFAQLDQRLGHLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPASARSPRAGDFGAAFVERYMREFGFIIPERQVVVDDVRVRGTGSSSLRLEDVPKAHSGPPRVDKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDIRISVGAETASVVGTQLDPIHLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKIPGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQGSAVFDFSGSGPEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPKGSILDPSPDAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRLGSGGRGRFRGGDGIIRELLFREEALLSVLTERRAFQPYGLMGGEPGARGLNLLIRKDGRTVNLGGKTSVPVYPGDVFCLHTPGGGGYGDPEDPAPLPGSPLQPLAFPERGSVYEYRRAQEAV

Target ORF information:

RefSeq Version	XM_025265601.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025265601.1

ORF Insert Sequence:

ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT 
GCCAACTATG CGGATGCCCC CACTGAGGGC ATCCGCCGTG TCCTGGAGCA GGAGGGGGGC 
GTGCTGCTGC CGCGGGACCG GCCACTGGAC ACAAGTCGCA TCGCCAGCAT CCGCATGGGC 
ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGAGGGGAG AGCGGGTGGC ACTGCTGGTG 
ACGCGTGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCTCGGA CCTCTTTGAC 
CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG 
GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCTGTGA AAGGCTGCAC AGGGGACCTA 
CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGGCTCCTG 
TCCCGGGGCA TCCGCAGCTT GGCTGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC 
GAGCAGCAGG TGGGAGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG 
GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC CGACGCCTAC 
CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGCC GTGGCTTCCA GGGTCAGCTC 
AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC 
AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCTGGGGGCG TGGTTGGTTA CTCAGCCACC 
ACCTACCGAG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC 
GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC 
ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACGGTGGCAG CGGGTGGGGG CTCCCGCCTC 
TTCTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCCGGCCCT 
GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG 
GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACAGAGG TCAACAGCTT CCTGACCAAT 
GGGCCTTACC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG 
GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC 
TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC 
CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG 
GGGCTGGCCC TGGCCGACGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATACT 
CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCACC TGGAGGAGCA GTGTGTGGAG 
GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG 
CGCTACCAGG GCACGGACTG CGCCTTGATG GTGTCTGCCC ACCAGCACCC AGCCAGTGCC 
CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC 
GGCTTCATTA TTCCCGAGCG GCAGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC 
AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT 
AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA 
GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC 
ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT 
GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACACAGCTTG ACCCCATCCA CCTTTCCATC 
TTCTCCCACC GCTTCATGAG CATCGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC 
ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG 
GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG 
CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGACGTGCT GCTGAGCAAC 
CACCCCAGCG CGGGGGGCAG CCACCTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG 
CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG 
GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC 
TTCCTGTCCT TTAAACTTGT GCAGGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC 
CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG 
TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC 
ATCGGGCAGT ACGGCCTGGA CGTGGTACAG GCCTACATGG GCCACATTCA GGCAAACGCG 
GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGCT CCCCTATCCG ACTCCGAGTG 
CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG 
TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC 
TGTCTGGTCG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG 
ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGATGCGG CCGTGGTGGG CGGCAACGTG 
CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCAGCTTCC 
CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG 
GCGGGCGGCG CGGGCGCGGG CCCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG 
ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC 
CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGACGGCATT 
ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTTTCCG TGCTGACCGA GCGCCGCGCC 
TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GAGGCCTAAA CCTACTCATC 
CGGAAGGACG GCCGGACAGT GAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCTGGG 
GACGTGTTCT GTCTCCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA GGACCCCGCC 
CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGCGGGGCAG CGTGTATGAG 
TACCGCAGGG CCCAGGAGGC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPLAH ( XM_025265602.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025121387.1 Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu49699
Clone ID Related Accession (Same CDS sequence)	XM_025265602.1
Accession Version	XM_025265602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	5-oxoprolinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
GGGGAGCCGG GTGCTGGGAC ACCTGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG 
CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GGCTCCTGTC CCGGGGCATC 
CGCAGCTTGG CTGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG 
GGAGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC 
ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCCG ACGCCTACCT CACGCCCACC 
ATCCAGCGCT ACGTGCAGGG CTTCCGCCGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG 
GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC 
GCTGTGCTCT CCGGCCCTGC TGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGAGTG 
GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC 
TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC 
CCCCAGCTGG ATATCAACAC GGTGGCAGCG GGTGGGGGCT CCCGCCTCTT CTTCAGGTCG 
GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CCGGCCCTGC CTGCTACCGA 
AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA 
AAGGCCCTGG AGGCTGTGGC CACAGAGGTC AACAGCTTCC TGACCAATGG GCCTTACCCG 
GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC 
ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG 
CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC 
ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG 
GCCGACGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATACTCC CGAGACCTTT 
GCGCAGCTGG ACCAGAGGCT GGGCCACCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC 
CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC 
ACGGACTGCG CCTTGATGGT GTCTGCCCAC CAGCACCCAG CCAGTGCCCG CTCACCCCGA 
GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT 
CCCGAGCGGC AGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT 
CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG 
TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT 
GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG 
CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG 
ACAGCCAGCG TGGTGGGCAC ACAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC 
TTCATGAGCA TCGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC 
ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC 
AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT 
CAGCAGTTGG GGGCTGATCT CCACCCCGGC GACGTGCTGC TGAGCAACCA CCCCAGCGCG 
GGGGGCAGCC ACCTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA 
CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA 
GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTGTCCTTT 
AAACTTGTGC AGGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA 
GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC 
GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC 
GGCCTGGACG TGGTACAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG 
AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ACCACATGGA CGACGGCTCC CCTATCCGAC TCCGAGTGCA GATCAACATG 
AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC 
AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTCGGC 
CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC 
TCCATCCTAG ACCCTTCCCC CGATGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG 
CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CAGCTTCCCA GGGCTGCATG 
AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGCGCG 
GGCGCGGGCC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC 
ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA 
AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ACGGCATTAT CCGTGAGCTG 
CTTTTCCGCG AGGAGGCGCT GCTTTCCGTG CTGACCGAGC GCCGCGCCTT CCAGCCGTAT 
GGCCTGATGG GGGGTGAGCC TGGTGCCCGA GGCCTAAACC TACTCATCCG GAAGGACGGC 
CGGACAGTGA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCTGGGGA CGTGTTCTGT 
CTCCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAGG ACCCCGCCCC ACTGCCAGGT 
TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG CGGGGCAGCG TGTATGAGTA CCGCAGGGCC 
CAGGAGGCTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025121387.1
CDS	198..3692
Translation	MPETLYEEVLEVDERVVLYRGEPGAGTPVKGCTGDLLEVQQPVDLGGLRGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGYSATTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVATEVNSFLTNGPYPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLPYTPETFAQLDQRLGHLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPASARSPRAGDFGAAFVERYMREFGFIIPERQVVVDDVRVRGTGSSSLRLEDVPKAHSGPPRVDKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDIRISVGAETASVVGTQLDPIHLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKIPGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQGSAVFDFSGSGPEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPKGSILDPSPDAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRLGSGGRGRFRGGDGIIRELLFREEALLSVLTERRAFQPYGLMGGEPGARGLNLLIRKDGRTVNLGGKTSVPVYPGDVFCLHTPGGGGYGDPEDPAPLPGSPLQPLAFPERGSVYEYRRAQEAV

Target ORF information:

RefSeq Version	XM_025265602.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025265602.1

ORF Insert Sequence:

ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
GGGGAGCCGG GTGCTGGGAC ACCTGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG 
CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GGCTCCTGTC CCGGGGCATC 
CGCAGCTTGG CTGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG 
GGAGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC 
ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCCG ACGCCTACCT CACGCCCACC 
ATCCAGCGCT ACGTGCAGGG CTTCCGCCGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG 
GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC 
GCTGTGCTCT CCGGCCCTGC TGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGAGTG 
GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC 
TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC 
CCCCAGCTGG ATATCAACAC GGTGGCAGCG GGTGGGGGCT CCCGCCTCTT CTTCAGGTCG 
GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CCGGCCCTGC CTGCTACCGA 
AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC 
TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA 
AAGGCCCTGG AGGCTGTGGC CACAGAGGTC AACAGCTTCC TGACCAATGG GCCTTACCCG 
GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC 
ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG 
CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC 
ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG 
GCCGACGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATACTCC CGAGACCTTT 
GCGCAGCTGG ACCAGAGGCT GGGCCACCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC 
CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC 
ACGGACTGCG CCTTGATGGT GTCTGCCCAC CAGCACCCAG CCAGTGCCCG CTCACCCCGA 
GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT 
CCCGAGCGGC AGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT 
CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG 
TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT 
GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG 
CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG 
ACAGCCAGCG TGGTGGGCAC ACAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC 
TTCATGAGCA TCGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC 
ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC 
AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT 
CAGCAGTTGG GGGCTGATCT CCACCCCGGC GACGTGCTGC TGAGCAACCA CCCCAGCGCG 
GGGGGCAGCC ACCTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA 
CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA 
GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTGTCCTTT 
AAACTTGTGC AGGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA 
GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC 
GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC 
GGCCTGGACG TGGTACAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG 
AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG 
TCTGCAGAGG ACCACATGGA CGACGGCTCC CCTATCCGAC TCCGAGTGCA GATCAACATG 
AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC 
AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTCGGC 
CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC 
TCCATCCTAG ACCCTTCCCC CGATGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG 
CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CAGCTTCCCA GGGCTGCATG 
AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGCGCG 
GGCGCGGGCC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC 
ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA 
AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ACGGCATTAT CCGTGAGCTG 
CTTTTCCGCG AGGAGGCGCT GCTTTCCGTG CTGACCGAGC GCCGCGCCTT CCAGCCGTAT 
GGCCTGATGG GGGGTGAGCC TGGTGCCCGA GGCCTAAACC TACTCATCCG GAAGGACGGC 
CGGACAGTGA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCTGGGGA CGTGTTCTGT 
CTCCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAGG ACCCCGCCCC ACTGCCAGGT 
TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG CGGGGCAGCG TGTATGAGTA CCGCAGGGCC 
CAGGAGGCTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OPLAH ( XM_006041071.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006041133.1 Bubalus bubalis 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X1, mRNA.

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CloneID	OBu00882
Clone ID Related Accession (Same CDS sequence)	XM_006041071.1
Accession Version	XM_006041071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	5-oxoprolinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005781533.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT 
GCCAACTATG CGGATGCCCC CACTGAGGGC ATCCGCCGTG TCCTGGAGCA GGAGGGGGGC 
GTGCTGCTGC CGCGGGACCG GCCACTGGAC ACAAGTCGCA TCGCCAGCAT CCGCATGGGC 
ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGAGGGGAG AGCGGGTGGC ACTGCTGGTG 
ACGCGTGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCTCGGA CCTCTTTGAC 
CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG 
GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCTGTGA AAGGCTGCAC AGGGGACCTA 
CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGGCTCCTG 
TCCCGGGGCA TCCGCAGCTT GGCTGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC 
GAGCAGCAGG TGGGAGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG 
GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC CGACGCCTAC 
CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGCC GTGGCTTCCA GGGTCAGCTC 
AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC 
AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCTGGGGGCG TGGTTGGTTA CTCAGCCACC 
ACCTACCGAG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC 
GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC 
ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACGGTGGCAG CGGGTGGGGG CTCCCGCCTC 
TTCTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCCGGCCCT 
GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG 
GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACAGAGG TCAACAGCTT CCTGACCAAT 
GGGCCTTACC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG 
GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC 
TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC 
CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG 
GGGCTGGCCC TGGCCGACGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATACT 
CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCACC TGGAGGAGCA GTGTGTGGAG 
GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG 
CGCTACCAGG GCACGGACTG CGCCTTGATG GTGTCTGCCC ACCAGCACCC AGCCAGTGCC 
CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC 
GGCTTCATTA TTCCCGAGCG GCAGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC 
AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT 
AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA 
GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC 
ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT 
GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACACAGCTTG ACCCCATCCA CCTTTCCATC 
TTCTCCCACC GCTTCATGAG CATCGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC 
ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG 
GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG 
CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGACGTGCT GCTGAGCAAC 
CACCCCAGCG CGGGGGGCAG CCACCTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG 
CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG 
GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC 
TTCCTGTCCT TTAAACTTGT GCAGGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC 
CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG 
TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC 
ATCGGGCAGT ACGGCCTGGA CGTGGTACAG GCCTACATGG GCCACATTCA GGCAAACGCG 
GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGCT CCCCTATCCG ACTCCGAGTG 
CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG 
TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC 
TGTCTGGTCG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG 
ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGATGCGG CCGTGGTGGG CGGCAACGTG 
CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCAGCTTCC 
CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG 
GCGGGCGGCG CGGGCGCGGG CCCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG 
ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC 
CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGACGGCATT 
ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTTTCCG TGCTGACCGA GCGCCGCGCC 
TTCCAGCCGT ATGGCCNNNC AGGGGGTGAG CCTGGTGCCC GAGGCCTAAA CCTACTCATC 
CGGAAGGACG GCCGGACAGT GAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCTGGG 
GACGTGTTCT GTCTCCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA GGACCCCGCC 
CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGCGGGGCAG CGTGTATGAG 
TACCGCAGGG CCCAGGAGGC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006041133.1
CDS	102..3968
Translation	MGGPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRVLEQEGGVLLPRDRPLDTSRIASIRMGTTVATNALLERRGERVALLVTRGFRDLLHVGTQARSDLFDLAVPMPETLYEEVLEVDERVVLYRGEPGAGTPVKGCTGDLLEVQQPVDLGGLRGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGALARELGFTHVSLSSEAMPMVRIVPRGHTACADAYLTPTIQRYVQGFRRGFQGQLKDVQVLFMRSDGGLAPMDSFSGSRAVLSGPAGGVVGYSATTYRVEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDINTVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGEDQPLSPEASRKALEAVATEVNSFLTNGPYPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGGAGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLPYTPETFAQLDQRLGHLEEQCVEALRAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPASARSPRAGDFGAAFVERYMREFGFIIPERQVVVDDVRVRGTGSSSLRLEDVPKAHSGPPRVDKMTQCYFEGGYQETPVYLLGELGCGHKLQGPCLIIDSNSTILVEPGCQAEVTETGDIRISVGAETASVVGTQLDPIHLSIFSHRFMSIAEQMGRILQRTAISTNIKERLDFSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQQLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFWPGQTRPVFYVASRGHHADIGGITPGSMPPHSTSLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKIPGCSGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTARQARGLPLEVSAEDHMDDGSPIRLRVQINMSQGSAVFDFSGSGPEVFGNLNAPRAITLSALIYCLRCLVGRDIPLNQGCLAPVRVVIPKGSILDPSPDAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETVAGGAGAGPGWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRLGSGGRGRFRGGDGIIRELLFREEALLSVLTERRAFQPYGnullnullGGEPGARGLNLLIRKDGRTVNLGGKTSVPVYPGDVFCLHTPGGGGYGDPEDPAPLPGSPLQPLAFPERGSVYEYRRAQEAV

Target ORF information:

RefSeq Version	XM_006041071.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006041071.1

ORF Insert Sequence:

ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT 
GCCAACTATG CGGATGCCCC CACTGAGGGC ATCCGCCGTG TCCTGGAGCA GGAGGGGGGC 
GTGCTGCTGC CGCGGGACCG GCCACTGGAC ACAAGTCGCA TCGCCAGCAT CCGCATGGGC 
ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGAGGGGAG AGCGGGTGGC ACTGCTGGTG 
ACGCGTGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCTCGGA CCTCTTTGAC 
CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG 
GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCTGTGA AAGGCTGCAC AGGGGACCTA 
CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGGCTCCTG 
TCCCGGGGCA TCCGCAGCTT GGCTGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC 
GAGCAGCAGG TGGGAGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG 
GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC CGACGCCTAC 
CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGCC GTGGCTTCCA GGGTCAGCTC 
AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC 
AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCTGGGGGCG TGGTTGGTTA CTCAGCCACC 
ACCTACCGAG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC 
GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC 
ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACGGTGGCAG CGGGTGGGGG CTCCCGCCTC 
TTCTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCCGGCCCT 
GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC 
CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG 
GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACAGAGG TCAACAGCTT CCTGACCAAT 
GGGCCTTACC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG 
GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC 
TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC 
CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG 
GGGCTGGCCC TGGCCGACGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATACT 
CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCACC TGGAGGAGCA GTGTGTGGAG 
GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG 
CGCTACCAGG GCACGGACTG CGCCTTGATG GTGTCTGCCC ACCAGCACCC AGCCAGTGCC 
CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC 
GGCTTCATTA TTCCCGAGCG GCAGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC 
AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT 
AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA 
GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC 
ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT 
GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACACAGCTTG ACCCCATCCA CCTTTCCATC 
TTCTCCCACC GCTTCATGAG CATCGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC 
ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG 
GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG 
CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGACGTGCT GCTGAGCAAC 
CACCCCAGCG CGGGGGGCAG CCACCTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG 
CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG 
GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC 
TTCCTGTCCT TTAAACTTGT GCAGGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC 
CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG 
TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC 
ATCGGGCAGT ACGGCCTGGA CGTGGTACAG GCCTACATGG GCCACATTCA GGCAAACGCG 
GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG 
CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGCT CCCCTATCCG ACTCCGAGTG 
CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG 
TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC 
TGTCTGGTCG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG 
ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGATGCGG CCGTGGTGGG CGGCAACGTG 
CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCAGCTTCC 
CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG 
GCGGGCGGCG CGGGCGCGGG CCCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG 
ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC 
CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGACGGCATT 
ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTTTCCG TGCTGACCGA GCGCCGCGCC 
TTCCAGCCGT ATGGCCNNNC AGGGGGTGAG CCTGGTGCCC GAGGCCTAAA CCTACTCATC 
CGGAAGGACG GCCGGACAGT GAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCTGGG 
GACGTGTTCT GTCTCCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA GGACCCCGCC 
CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGCGGGGCAG CGTGTATGAG 
TACCGCAGGG CCCAGGAGGC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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OPLAH cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム