LOC103865560 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103865560
Entrez Gene ID	103865560
Full Name	calcium-dependent protein kinase 12-like
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009143365.2	XP_009141613.1	calcium-dependent protein kinase 12-like

Pathways from BioSystems
Gene Ontology

The following LOC103865560 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103865560 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr127880	XM_009143365.2 Latest version!	Brassica rapa calcium-dependent protein kinase 12-like (LOC103865560), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103865560 ( XM_009143365.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009141613.1 Brassica rapa calcium-dependent protein kinase 12-like (LOC103865560), mRNA.

Price & Availability↑

CloneID	OBr127880
Clone ID Related Accession (Same CDS sequence)	XM_009143365.2
Accession Version	XM_009143365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	calcium-dependent protein kinase 12-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024795.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009143365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAACA AATCAAGAAC CAGATGGGTT CTCCCTTACA CAACCAAGAA CGTGGAAGAC 
GATTACTTCC TCGGTCAAAT CCTCGGACAA GGACAATTCG GAACCACTTT CCTCTGCACC 
CACAAAAAGA CAGCTCAAAA GCTCGCCTGC AAATCCATAC CCAAAAGGAA GCTCCTCTGC 
CAAGAAGACT ACGACGACGT TTTGAGGGAG ATTCAGATAA TGCATCACTT GTCGGAATTC 
CCCAACGTTG TCCGGATAGA AGGCGCCTAC GAGGATACCA GCAGCGTCCA TATCGTGATG 
GAGCTCTGTG AAGGCGGCGA GCTGTTTGAT AGGATCGTGA AGAGAGGTCA TTACAGTGAG 
AGGGAAGCAG CTAAGCTTAT CAAGACGATT GTTGGTGTTG TTGAGGCTTG TCACTCTCTT 
GGTGTTATGC ATAGAGATCT CAAGCCTGAG AATTTTCTCT TTGCTTCTTG TGATGAAGAT 
GCTTCCCTTA AGTCTACTGA CTTTGGCCTC TCTGTTTTCT GCAAGCCAGG GGCAACGTTT 
TCAGAACTAG TTGGGAGTGC ATACTATGTA GCACCTGAGG TTTTACATAA GCACTACAGC 
CGTGAATGTG ATGTATGGAG CGCTGGAGTT ATCCTCTACA TTCTCTTATG TGGTTTCCCT 
CCTTTCTGGG ATGAAAGTGA GTTTGGCATC TTCAGAAAGA TTCTACAGGG AAAAATTGAC 
TTTGAGACAA GTCCCTGGCC TAGCATCTCA GAGAGTGCCA AGGATCTTAT AATGAAAATG 
CTCGAGAAAG ATCCAAAGAA AAGGCTAACT GCTCATCAAG TGTTGTGTCA TCCCTGGATT 
GTGGATGATA AGGTTGCTCC AGATAAACCA TTGGACTGTG CAGTACTGTC ACGCTTGAAG 
AACTTCTCTG CAATGAACAA ACTTAAGAAG ATGGCTTTTC GAGTGATCGC AGAGAGACTA 
TCTGAGGAAG AGATAGGTGG ACTCAAAGAG CTCTTCAGAA TGATAGACAC AGATAACAGT 
GGAACCATCA CTTTTGAAGA GTTGAAAGAC ACTGTGAAGC GTGTTGGTGC AGACCTTATG 
GAATCAGAGA TCCAAGAACT CTTGCGCTCA GCTGATGTTG ATGAAAATGG ATCAATAGAC 
TACGGAGAGT TTTTAGCTGC AACAATCCAC TTGAACAAGC TGGAGAGAGA GGAGAATCTA 
GTGGCTGCAT TCTCTTTCTT TGACAAAGAT GGAAGTGGCT ACATCACTGT GGATGAGCTT 
CAGCAGGCTT TGAAGGAGTT TGGTATAAAT GATTCACATC TTGATGAAAT GATCAAAGAC 
ATTGATCAAG ACAATGATGG ACAAATAGAC TATGGAGAGT TTGTGGCAAT GATGAGGAAA 
GGAAATGGCA GTGGAGGGAT TGGCAGGAGA ACAATGAGGA ACACTCTCAA CTTTGCAAAT 
TGGAAAGTAG CCTCTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009141613.1
CDS	179..1639
Translation	MANKSRTRWVLPYTTKNVEDDYFLGQILGQGQFGTTFLCTHKKTAQKLACKSIPKRKLLCQEDYDDVLREIQIMHHLSEFPNVVRIEGAYEDTSSVHIVMELCEGGELFDRIVKRGHYSEREAAKLIKTIVGVVEACHSLGVMHRDLKPENFLFASCDEDASLKSTDFGLSVFCKPGATFSELVGSAYYVAPEVLHKHYSRECDVWSAGVILYILLCGFPPFWDESEFGIFRKILQGKIDFETSPWPSISESAKDLIMKMLEKDPKKRLTAHQVLCHPWIVDDKVAPDKPLDCAVLSRLKNFSAMNKLKKMAFRVIAERLSEEEIGGLKELFRMIDTDNSGTITFEELKDTVKRVGADLMESEIQELLRSADVDENGSIDYGEFLAATIHLNKLEREENLVAAFSFFDKDGSGYITVDELQQALKEFGINDSHLDEMIKDIDQDNDGQIDYGEFVAMMRKGNGSGGIGRRTMRNTLNFANWKVASS

Target ORF information:

RefSeq Version	XM_009143365.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa calcium-dependent protein kinase 12-like (LOC103865560), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009143365.2

ORF Insert Sequence:

ATGGCTAACA AATCAAGAAC CAGATGGGTT CTCCCTTACA CAACCAAGAA CGTGGAAGAC 
GATTACTTCC TCGGTCAAAT CCTCGGACAA GGACAATTCG GAACCACTTT CCTCTGCACC 
CACAAAAAGA CAGCTCAAAA GCTCGCCTGC AAATCCATAC CCAAAAGGAA GCTCCTCTGC 
CAAGAAGACT ACGACGACGT TTTGAGGGAG ATTCAGATAA TGCATCACTT GTCGGAATTC 
CCCAACGTTG TCCGGATAGA AGGCGCCTAC GAGGATACCA GCAGCGTCCA TATCGTGATG 
GAGCTCTGTG AAGGCGGCGA GCTGTTTGAT AGGATCGTGA AGAGAGGTCA TTACAGTGAG 
AGGGAAGCAG CTAAGCTTAT CAAGACGATT GTTGGTGTTG TTGAGGCTTG TCACTCTCTT 
GGTGTTATGC ATAGAGATCT CAAGCCTGAG AATTTTCTCT TTGCTTCTTG TGATGAAGAT 
GCTTCCCTTA AGTCTACTGA CTTTGGCCTC TCTGTTTTCT GCAAGCCAGG GGCAACGTTT 
TCAGAACTAG TTGGGAGTGC ATACTATGTA GCACCTGAGG TTTTACATAA GCACTACAGC 
CGTGAATGTG ATGTATGGAG CGCTGGAGTT ATCCTCTACA TTCTCTTATG TGGTTTCCCT 
CCTTTCTGGG ATGAAAGTGA GTTTGGCATC TTCAGAAAGA TTCTACAGGG AAAAATTGAC 
TTTGAGACAA GTCCCTGGCC TAGCATCTCA GAGAGTGCCA AGGATCTTAT AATGAAAATG 
CTCGAGAAAG ATCCAAAGAA AAGGCTAACT GCTCATCAAG TGTTGTGTCA TCCCTGGATT 
GTGGATGATA AGGTTGCTCC AGATAAACCA TTGGACTGTG CAGTACTGTC ACGCTTGAAG 
AACTTCTCTG CAATGAACAA ACTTAAGAAG ATGGCTTTTC GAGTGATCGC AGAGAGACTA 
TCTGAGGAAG AGATAGGTGG ACTCAAAGAG CTCTTCAGAA TGATAGACAC AGATAACAGT 
GGAACCATCA CTTTTGAAGA GTTGAAAGAC ACTGTGAAGC GTGTTGGTGC AGACCTTATG 
GAATCAGAGA TCCAAGAACT CTTGCGCTCA GCTGATGTTG ATGAAAATGG ATCAATAGAC 
TACGGAGAGT TTTTAGCTGC AACAATCCAC TTGAACAAGC TGGAGAGAGA GGAGAATCTA 
GTGGCTGCAT TCTCTTTCTT TGACAAAGAT GGAAGTGGCT ACATCACTGT GGATGAGCTT 
CAGCAGGCTT TGAAGGAGTT TGGTATAAAT GATTCACATC TTGATGAAAT GATCAAAGAC 
ATTGATCAAG ACAATGATGG ACAAATAGAC TATGGAGAGT TTGTGGCAAT GATGAGGAAA 
GGAAATGGCA GTGGAGGGAT TGGCAGGAGA ACAATGAGGA ACACTCTCAA CTTTGCAAAT 
TGGAAAGTAG CCTCTTCCTG A