LOC103861305 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103861305
Entrez Gene ID	103861305
Full Name	alkaline ceramidase 3-like
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009139008.2	XP_009137256.1	alkaline ceramidase 3-like

Pathways from BioSystems
Gene Ontology

The following LOC103861305 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103861305 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr142750	XM_009139008.2 Latest version!	Brassica rapa alkaline ceramidase 3-like (LOC103861305), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103861305 ( XM_009139008.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009137256.1 Brassica rapa alkaline ceramidase 3-like (LOC103861305), mRNA.

Price & Availability↑

CloneID	OBr142750
Clone ID Related Accession (Same CDS sequence)	XM_009139008.2
Accession Version	XM_009139008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	alkaline ceramidase 3-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024797.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009139008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG GGATATCTAG CTTTTGGGGT CCCGTGACTT CCACCATAGA GTGTTGTGAG 
AAGAACTACG CATACTCCTC TTACATTGCA GAGTTCTACA ACACCATCTC CAACGTCCCT 
GGAATCCTCT TGGCCCTCAT TGGTCTCGTC AATGCGTTGA GGCAACGGTT CGAGAAGAGG 
TTCAGCGTCC TCCACATATC CAACATGATC CTCGCTATCG GCAGCATGCT CTACCATGCC 
ACTTTGCAGC ACGTGCAACA GCAGAGTGAC GAGACGCCGA TGGTGTGGGA GATACTTCTC 
TACATGTACA TCCTTTACTC ACCAGACTGG CATTACAGAA GCACTATGCC AACTTTTCTC 
TTCCTCTACG GAGCTGCCTT TGCTGCTGCC CACGCTTTCC TCAGGTTTGG GATCGGTTTC 
AAGGTCCATT ACGTGATCCT CTGCCTTCTC TGCATCCCTC GGATGTACAA GTACTACATT 
CACACGGAGG ACACTGCGGC TAAAAGGATT GCGAAGTGGT ATGTTGCCAC GATCCTTGTG 
GGTAGTGTTT GCTGGTTCTG TGACCGTGTT TTCTGCAAGA GGATATCTCA GTGGCCTGTG 
AACCCTCAGG GACATGCTCT GTGGCATGTG TTTATGAGTT TCAACTCGTA CTGTGCGAAC 
ACGTTCTTGA TGTTCTGTCG AGCTCAGCAG CGTGGATGGA ATCCAAAGGT TAAGTACTTT 
CTGGGGGTTC TTCCTTACGT CAAGATCGAG AAGCCAAAAG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009137256.1
CDS	217..984
Translation	MADGISSFWGPVTSTIECCEKNYAYSSYIAEFYNTISNVPGILLALIGLVNALRQRFEKRFSVLHISNMILAIGSMLYHATLQHVQQQSDETPMVWEILLYMYILYSPDWHYRSTMPTFLFLYGAAFAAAHAFLRFGIGFKVHYVILCLLCIPRMYKYYIHTEDTAAKRIAKWYVATILVGSVCWFCDRVFCKRISQWPVNPQGHALWHVFMSFNSYCANTFLMFCRAQQRGWNPKVKYFLGVLPYVKIEKPKEQ

Target ORF information:

RefSeq Version	XM_009139008.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa alkaline ceramidase 3-like (LOC103861305), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009139008.2

ORF Insert Sequence:

ATGGCAGATG GGATATCTAG CTTTTGGGGT CCCGTGACTT CCACCATAGA GTGTTGTGAG 
AAGAACTACG CATACTCCTC TTACATTGCA GAGTTCTACA ACACCATCTC CAACGTCCCT 
GGAATCCTCT TGGCCCTCAT TGGTCTCGTC AATGCGTTGA GGCAACGGTT CGAGAAGAGG 
TTCAGCGTCC TCCACATATC CAACATGATC CTCGCTATCG GCAGCATGCT CTACCATGCC 
ACTTTGCAGC ACGTGCAACA GCAGAGTGAC GAGACGCCGA TGGTGTGGGA GATACTTCTC 
TACATGTACA TCCTTTACTC ACCAGACTGG CATTACAGAA GCACTATGCC AACTTTTCTC 
TTCCTCTACG GAGCTGCCTT TGCTGCTGCC CACGCTTTCC TCAGGTTTGG GATCGGTTTC 
AAGGTCCATT ACGTGATCCT CTGCCTTCTC TGCATCCCTC GGATGTACAA GTACTACATT 
CACACGGAGG ACACTGCGGC TAAAAGGATT GCGAAGTGGT ATGTTGCCAC GATCCTTGTG 
GGTAGTGTTT GCTGGTTCTG TGACCGTGTT TTCTGCAAGA GGATATCTCA GTGGCCTGTG 
AACCCTCAGG GACATGCTCT GTGGCATGTG TTTATGAGTT TCAACTCGTA CTGTGCGAAC 
ACGTTCTTGA TGTTCTGTCG AGCTCAGCAG CGTGGATGGA ATCCAAAGGT TAAGTACTTT 
CTGGGGGTTC TTCCTTACGT CAAGATCGAG AAGCCAAAAG AACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.