LOC109481725 cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109481725
Entrez Gene ID	109481725
Full Name	collagen alpha-1(I) chain-like
Gene Type	protein-coding
Organism	Branchiostoma belcheri(Belcher's lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019784316.1	XP_019639875.1	collagen alpha-1(I) chain-like

Pathways from BioSystems
Gene Ontology

The following LOC109481725 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109481725 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr191856	XM_019784316.1 Latest version!	Branchiostoma belcheri collagen alpha-1(I) chain-like (LOC109481725), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109481725 ( XM_019784316.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019639875.1 Branchiostoma belcheri collagen alpha-1(I) chain-like (LOC109481725), mRNA.

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CloneID	OBr191856
Clone ID Related Accession (Same CDS sequence)	XM_019784316.1
Accession Version	XM_019784316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	collagen alpha-1(I) chain-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017804209.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGCTGA CGATTGACCA GATCCTGTCG GAGACGAAGA AGCTGGTTGA CCGGCTCCGA 
GACCACGACA CGGCCGCGGA CGGCCTGATT GGTCAGGCTC AGGTCCTCAA CAAGCGCATT 
GATGCCATGA AACAGTACCA GGAGGACATG AATGAGCTGA ACGAAGTCGC CCGACACAAA 
CCGCGATCAA CGCTGATCCT CGGCATCCAG CAGGAGAACA GGCAGATACG GGAACTGCAG 
CAGGAAAACA AGGAGCTGCG GGTTTCCCTG CAGGAGCACC AGGCTGCCCT GGAGCTCATC 
ATGAGCAAAT ATCGGGAGCA GATCCTGAAA CTGGTCGTCG CAAATCGACT CGAGAAGCCC 
TGGACGAGAC TTCAGCTGGC TGCAACACCG CAGCACTCTG CCGAGGTCCA AACAAAGATT 
GAGAAGATTT GTGAGATGGC AGCTGTCATG GCCAAAGCCG TTGCTATGGA CGATGAGTAT 
GTTGCTAAGG AACAGGAGAG AGTGGCACAG CTAGAGGTGG AGAACAAGGG ACTGAGGGAA 
CTTCTGGAGA TCAGCACGAA ACTCAAGGTC ACACACTCGG GTGAAGGTCG CAGGGAGGAC 
AAGCCCCCTC CCCCGTACGT CCCCCGACAG ACCCACGTAC TTCTCAAAGA CAAGGAGCCA 
CCGGCAACAA CTGTGTCTGA CAGGACAGCT CAACCAAAGG GGCCACCTAG CAGGGATGGG 
CTTCCTAGCA GGGATGGGCC ACCTAGCAGG GATGGGCCAC CCAACAGGGA TGGGCCACCT 
AGCAGGGTTG GACTGCCTAG CAGCGATGGA CCACCTAGCG TAGAGGGGTT ACCTAGCAGG 
GATGCACCAC CTAGCAGGGT TGGACCGCCT AGCATAGAGG GGCCACCTAG CAGGGATGGA 
CCACCCAGCA GGGATGGACC ACCTGGACCA CCTAGCAGGG ATGGGCCACC TAGCAGGGAT 
GAGCTGCCTA GCAGGGATGG ACCACCTAGC AGGGATGGGC CACCTACCAG TGGTGGGCCA 
CCTAGCATAG AGGGGCCACC TAGCAGGGAT GGGGCACCTA GCAGGGATGG GCCACCTAGC 
AGGGATGGAC CACCTAGCAG GGATGGGCCA CCTAGCGTAG AGGGGCCACC TAGCAGAGAT 
GGGCCAACTA GCAGGGATGG ACCACCTAGC GTAGAGGGGC CACCTAGCAG GGATGGGCCA 
CCTAGTACAG GGGGTCTACA TGGCAGGGAC AGGCCACAGG GTTCAGAAGG GTCAGAACAG 
ACAGGAGAAG TAGTGAGTAG CATTGTAGAG GTCAGTCTTG TAGAACAGCT AAGGAGGGAG 
GCGTTAGCTG GTTCACAGGA CATCTCCAGC GAGAAGGTAC CTACCCTTCC GGCGGGCCTT 
GTCTCTCCTC AAGCCAACAC TCAGGAACAA ACAGACATGG GAGAAACAAC TACAGATACA 
AGACAAGACG CTTCACCACC AACGATGCAG GAAACCGGCA CCGAAACAGG ACAAGATGCA 
ACAGAAACAA GACATGTCTC GGGTGCACAA CTTCAGGTGC AGGCAGAGGT GACTGTCTTG 
TCAGAGGATG AAAGCGAGAC AGACATCTTG GAAAGTGAGA GTGACACTAC TATAGAGAGT 
GAAACTGAAG TAGAAAGTGA CAGTGAACTA GTTGTAAATA AAACGGAAGT TATGGAGAGT 
GAGTCCACGT GTACAGCGCC AGATGTGGGC AGCTCAGGGA GAAACACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019639875.1
CDS	76..1806
Translation	MTLTIDQILSETKKLVDRLRDHDTAADGLIGQAQVLNKRIDAMKQYQEDMNELNEVARHKPRSTLILGIQQENRQIRELQQENKELRVSLQEHQAALELIMSKYREQILKLVVANRLEKPWTRLQLAATPQHSAEVQTKIEKICEMAAVMAKAVAMDDEYVAKEQERVAQLEVENKGLRELLEISTKLKVTHSGEGRREDKPPPPYVPRQTHVLLKDKEPPATTVSDRTAQPKGPPSRDGLPSRDGPPSRDGPPNRDGPPSRVGLPSSDGPPSVEGLPSRDAPPSRVGPPSIEGPPSRDGPPSRDGPPGPPSRDGPPSRDELPSRDGPPSRDGPPTSGGPPSIEGPPSRDGAPSRDGPPSRDGPPSRDGPPSVEGPPSRDGPTSRDGPPSVEGPPSRDGPPSTGGLHGRDRPQGSEGSEQTGEVVSSIVEVSLVEQLRREALAGSQDISSEKVPTLPAGLVSPQANTQEQTDMGETTTDTRQDASPPTMQETGTETGQDATETRHVSGAQLQVQAEVTVLSEDESETDILESESDTTIESETEVESDSELVVNKTEVMESESTCTAPDVGSSGRNT

Target ORF information:

RefSeq Version	XM_019784316.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri collagen alpha-1(I) chain-like (LOC109481725), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019784316.1

ORF Insert Sequence:

ATGACGCTGA CGATTGACCA GATCCTGTCG GAGACGAAGA AGCTGGTTGA CCGGCTCCGA 
GACCACGACA CGGCCGCGGA CGGCCTGATT GGTCAGGCTC AGGTCCTCAA CAAGCGCATT 
GATGCCATGA AACAGTACCA GGAGGACATG AATGAGCTGA ACGAAGTCGC CCGACACAAA 
CCGCGATCAA CGCTGATCCT CGGCATCCAG CAGGAGAACA GGCAGATACG GGAACTGCAG 
CAGGAAAACA AGGAGCTGCG GGTTTCCCTG CAGGAGCACC AGGCTGCCCT GGAGCTCATC 
ATGAGCAAAT ATCGGGAGCA GATCCTGAAA CTGGTCGTCG CAAATCGACT CGAGAAGCCC 
TGGACGAGAC TTCAGCTGGC TGCAACACCG CAGCACTCTG CCGAGGTCCA AACAAAGATT 
GAGAAGATTT GTGAGATGGC AGCTGTCATG GCCAAAGCCG TTGCTATGGA CGATGAGTAT 
GTTGCTAAGG AACAGGAGAG AGTGGCACAG CTAGAGGTGG AGAACAAGGG ACTGAGGGAA 
CTTCTGGAGA TCAGCACGAA ACTCAAGGTC ACACACTCGG GTGAAGGTCG CAGGGAGGAC 
AAGCCCCCTC CCCCGTACGT CCCCCGACAG ACCCACGTAC TTCTCAAAGA CAAGGAGCCA 
CCGGCAACAA CTGTGTCTGA CAGGACAGCT CAACCAAAGG GGCCACCTAG CAGGGATGGG 
CTTCCTAGCA GGGATGGGCC ACCTAGCAGG GATGGGCCAC CCAACAGGGA TGGGCCACCT 
AGCAGGGTTG GACTGCCTAG CAGCGATGGA CCACCTAGCG TAGAGGGGTT ACCTAGCAGG 
GATGCACCAC CTAGCAGGGT TGGACCGCCT AGCATAGAGG GGCCACCTAG CAGGGATGGA 
CCACCCAGCA GGGATGGACC ACCTGGACCA CCTAGCAGGG ATGGGCCACC TAGCAGGGAT 
GAGCTGCCTA GCAGGGATGG ACCACCTAGC AGGGATGGGC CACCTACCAG TGGTGGGCCA 
CCTAGCATAG AGGGGCCACC TAGCAGGGAT GGGGCACCTA GCAGGGATGG GCCACCTAGC 
AGGGATGGAC CACCTAGCAG GGATGGGCCA CCTAGCGTAG AGGGGCCACC TAGCAGAGAT 
GGGCCAACTA GCAGGGATGG ACCACCTAGC GTAGAGGGGC CACCTAGCAG GGATGGGCCA 
CCTAGTACAG GGGGTCTACA TGGCAGGGAC AGGCCACAGG GTTCAGAAGG GTCAGAACAG 
ACAGGAGAAG TAGTGAGTAG CATTGTAGAG GTCAGTCTTG TAGAACAGCT AAGGAGGGAG 
GCGTTAGCTG GTTCACAGGA CATCTCCAGC GAGAAGGTAC CTACCCTTCC GGCGGGCCTT 
GTCTCTCCTC AAGCCAACAC TCAGGAACAA ACAGACATGG GAGAAACAAC TACAGATACA 
AGACAAGACG CTTCACCACC AACGATGCAG GAAACCGGCA CCGAAACAGG ACAAGATGCA 
ACAGAAACAA GACATGTCTC GGGTGCACAA CTTCAGGTGC AGGCAGAGGT GACTGTCTTG 
TCAGAGGATG AAAGCGAGAC AGACATCTTG GAAAGTGAGA GTGACACTAC TATAGAGAGT 
GAAACTGAAG TAGAAAGTGA CAGTGAACTA GTTGTAAATA AAACGGAAGT TATGGAGAGT 
GAGTCCACGT GTACAGCGCC AGATGTGGGC AGCTCAGGGA GAAACACATG A