LOC109470275 cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109470275
Entrez Gene ID	109470275
Full Name	AP-2 complex subunit alpha-2-like
Gene Type	protein-coding
Organism	Branchiostoma belcheri(Belcher's lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019769160.1	XP_019624719.1	AP-2 complex subunit alpha-2-like isoform X1
XM_019769161.1	XP_019624720.1	AP-2 complex subunit alpha-2-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC109470275 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109470275 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr179608	XM_019769160.1 Latest version!	Branchiostoma belcheri AP-2 complex subunit alpha-2-like (LOC109470275), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBr179609	XM_019769161.1 Latest version!	Branchiostoma belcheri AP-2 complex subunit alpha-2-like (LOC109470275), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109470275 ( XM_019769160.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019624719.1 Branchiostoma belcheri AP-2 complex subunit alpha-2-like (LOC109470275), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBr179608
Clone ID Related Accession (Same CDS sequence)	XM_019769160.1
Accession Version	XM_019769160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	AP-2 complex subunit alpha-2-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017803713.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAACG TGGCGAAGGG AGACGGGATG CGCGGCCTCG CCGTGTTCAT CTCCGACATC 
AGAAACTGTA AAAGCAAAGA AGCAGAGCAG AAGCGGATAA ACAAGGAACT GGCAAACATT 
AGATCCAAAT TCAAAGGTGA TAAGACCCTG GATGGATACA GTAAGAAGAA GTATGTGTGT 
AAACTGCTCT TCATTTTCCT GCTGGGACAT GACATTGACT TTGGGCACAT GGAGGCCGTC 
AACCTCCTGT CTTCCAACAA GTACACCGAG AAACAGATCG GTTATCTGTT TATCTCCGTG 
CTGGTCAACA CCAACAGTGA CCTGATCCGT CTGATTATCG GCTCCATCAA GAACGACCTC 
CTGTCCCGTA ACGCTGTCCA CATCTGCCTG GCCCTGCAGT GTATCGCCAA CATCGGCAGT 
AAGGAGATGG CCGAGTCACT CGCCCCCGAG ATCCCCAAAC TCATCGTGTC AGGGGAGACC 
ATTGACGCGG TGAAGCAGAG CGCCTGCCTG TGTCTGCTGC GGCTCTTCCG CACATCGCCC 
GACACCGTGC CATCCGGCGA ATGGACGCAG AGGGTCGGCC ATCTTCTCAA CGACCAACAT 
CTGGGTGTAG TAACAGCTGC CACCAGTCTG ATCCACACTC TGGCACAGAA GACCCCGGAG 
GACTTCAAGA ATTGTGTTTC CCTGGCCATC TCTCGACTCA GTAGGATTGT GACCTCATCA 
TACACAGACC TCCAGGACTA CACCTACTAC TTTGTCCCTG CCCCCTGGCT GTCAGTAAAG 
CTACTCAGGC TGCTGCAAGT CTACCCACCA CCAGAGGACC CAGCGGCACG TGGTCGGCTG 
AACGAGTGTC TGGAGACCAT CCTGAACAAG GCCCAGGAAC CTCCCAAGTC TAAGAAGGTG 
CAGCACTCCA ACGCTAAGAA CGCTGTGCTG TTTGAGGCCA TCAACCTTAT TATTCACCAT 
GACACAGAAC CCACCCTGTT GGTGCGTGCG TGTAACCAGC TGGGCCAGTT CTTGCAGCAC 
AGAGAGACTA ACCTGCGGTA CCTGGCCCTG GAGAGCATGT GTCTGCTGGC CTCCTCCGAG 
TTCTCACACG AGGCAGTCAA GAAACACATG GACACGGTCA TCACCGCACT CAAGACTGAG 
CGTGACGTGA GCGTACGACA GCGAGCTGTA GATCTGCTGT ACGGGATGTG TGACAAGTCC 
AACGCTGAGG AGATCGTGGC CGAGATGCTG GCCTACCTGG AGACGGCCGA CTACTCCATC 
AGAGAGGAAA TGGTGTTGAA GGTGGCCATC TTGGCAGAAA AGTACCCCGT TGACTACACC 
TGGTACGTGG ACACCATCCT GAACCTGATC CGAATTGCTG GAGACTACGT CAGTGACGAG 
GTGTGGTTCC GTGTCATCCA GATTGTCATC AACAGGGACG ATGTGCAAGG GTATGCTGCA 
AAGGTCGTGT TTGAGGCACT CCAAGCGCCA GCCTGCCATG AGAACATGGT GAAAGTAGGA 
GGATATATCC TCGGGGAGTT CGGCAATCTC ATCGCAGGAG ACCAGAGATC CAGCCCGATG 
GTCCAGTTCC AGCTGCTGCA CTCCAAGTAC CACCTGTGCA GCGCCCCGAC CAGGGGACTT 
CTCCTCTCCA CATACATCAA GTTCATCAAC CTGTTCCCAG AGATCAAGGG CCAGCTCCAG 
GACGTGTTGC GGAATAACAA CAACCTGCGA AATGCGGACG CAGAGCTGCA GCAGAGAGCA 
ATCGAGTACC TACAGCTCAG TACGGTAGCC AGTCAGGACG TCCTGGCGAC AGTTCTAGAA 
GAAATGCCCC CCTTCCCTGA GAGAGAGTCG TCCATCCTGG CCAAGCTGAA GAAGAAGAAG 
CCCAACGTCT CAGACATCGA GGTGGTGAAG GAGGGCAAGA AGTCATCCAA CCCCCCCACC 
CTCAACAACA GCGCAGCCAA CACACCTGAG GCAACACCTG CATCAGCACC TGTACCTGTC 
ATGGCGGCTG CACCTGCTCC TGTCCAGAGT GCCTCTGATG ACCTGCTTGG TCTGAGCGCC 
GGTCCGGCCG CCGCTGCGCC CATTACCAAC AACTTCGCAG CCCCGGCGTC GTTCGCGGCT 
CCGGCGGCGC CCGCGGGAGG TGGCGGACTG CTCGTGGACG TGTTCGACAC GCCGCCTGCC 
CAGAACAACG TGAGCTCAGC GTTAGCGCCA CAAGCCGAGG AGAACTTCTT TAATGCGCCC 
GCCGGCGAAA CTGCGCCCTC CGGTGACACC CCTGCTTCGG AGCTTGACGG AAGGTTTGCG 
ATTAAGAACA ACGGTGTCCT GTTTGAGAAT GAACTCCTGC AGATCGGGGT CAAAGCAGAG 
TACAAGGGCA ACCTGGGACG CCTGGGCCTG TTCTATGGAA ACAAGACGTC GTACCCCTTC 
CAGTTCTTCA CTCCCACCGT GTCTTGTCCA GGAGAGCTGG CCTCTGCGTT GAATGTCCAG 
ACCAAACCCC TCGACGTGAA CACAGTGGAG GGAGGAGCGC AGGTCCAACA AATCATCAAC 
GTGGAGTGTA TATCAGACTT TGTGGACGCA CCCGTCCTCA GCATTAAGTT CAGTTACTCA 
GGCAGTATGC AGCAGATCAA CCTGATGCTG CCTGTCATGT TGAACAAGTT CTTTGAACCC 
GTGGAGATGG ACGCCACCAC TTTCTTCAAC AGGTGGAAAC AGCTCAACAG CCCTGGCCAG 
GAGGAGCAAA AGATTTTCAA GGCTACGTTC CCAATAGACT CGGATACCTC CAGGACAAAG 
CTTCTTGGCT TTGGGTTTGG ACTCCTGGAC GGGGTAGATC CGAACCCAGA CAACTTTGTC 
GGAGCAGGAA TCATCAAGGC ACAGTCCTCA CAGGTCGGCT GTCTGCTCAG ACTAGAGCCC 
AACATGCAAG CACAGATGTA CCGGTTAACT TTGCGAACCA GCAGCACGTC TGTCACCACA 
AGGCTGGCTG ATCTCCTTCT CAAGCTTTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019624719.1
CDS	281..3253
Translation	MPNVAKGDGMRGLAVFISDIRNCKSKEAEQKRINKELANIRSKFKGDKTLDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNTNSDLIRLIIGSIKNDLLSRNAVHICLALQCIANIGSKEMAESLAPEIPKLIVSGETIDAVKQSACLCLLRLFRTSPDTVPSGEWTQRVGHLLNDQHLGVVTAATSLIHTLAQKTPEDFKNCVSLAISRLSRIVTSSYTDLQDYTYYFVPAPWLSVKLLRLLQVYPPPEDPAARGRLNECLETILNKAQEPPKSKKVQHSNAKNAVLFEAINLIIHHDTEPTLLVRACNQLGQFLQHRETNLRYLALESMCLLASSEFSHEAVKKHMDTVITALKTERDVSVRQRAVDLLYGMCDKSNAEEIVAEMLAYLETADYSIREEMVLKVAILAEKYPVDYTWYVDTILNLIRIAGDYVSDEVWFRVIQIVINRDDVQGYAAKVVFEALQAPACHENMVKVGGYILGEFGNLIAGDQRSSPMVQFQLLHSKYHLCSAPTRGLLLSTYIKFINLFPEIKGQLQDVLRNNNNLRNADAELQQRAIEYLQLSTVASQDVLATVLEEMPPFPERESSILAKLKKKKPNVSDIEVVKEGKKSSNPPTLNNSAANTPEATPASAPVPVMAAAPAPVQSASDDLLGLSAGPAAAAPITNNFAAPASFAAPAAPAGGGGLLVDVFDTPPAQNNVSSALAPQAEENFFNAPAGETAPSGDTPASELDGRFAIKNNGVLFENELLQIGVKAEYKGNLGRLGLFYGNKTSYPFQFFTPTVSCPGELASALNVQTKPLDVNTVEGGAQVQQIINVECISDFVDAPVLSIKFSYSGSMQQINLMLPVMLNKFFEPVEMDATTFFNRWKQLNSPGQEEQKIFKATFPIDSDTSRTKLLGFGFGLLDGVDPNPDNFVGAGIIKAQSSQVGCLLRLEPNMQAQMYRLTLRTSSTSVTTRLADLLLKLF

Target ORF information:

RefSeq Version	XM_019769160.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri AP-2 complex subunit alpha-2-like (LOC109470275), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019769160.1

ORF Insert Sequence:

ATGCCCAACG TGGCGAAGGG AGACGGGATG CGCGGCCTCG CCGTGTTCAT CTCCGACATC 
AGAAACTGTA AAAGCAAAGA AGCAGAGCAG AAGCGGATAA ACAAGGAACT GGCAAACATT 
AGATCCAAAT TCAAAGGTGA TAAGACCCTG GATGGATACA GTAAGAAGAA GTATGTGTGT 
AAACTGCTCT TCATTTTCCT GCTGGGACAT GACATTGACT TTGGGCACAT GGAGGCCGTC 
AACCTCCTGT CTTCCAACAA GTACACCGAG AAACAGATCG GTTATCTGTT TATCTCCGTG 
CTGGTCAACA CCAACAGTGA CCTGATCCGT CTGATTATCG GCTCCATCAA GAACGACCTC 
CTGTCCCGTA ACGCTGTCCA CATCTGCCTG GCCCTGCAGT GTATCGCCAA CATCGGCAGT 
AAGGAGATGG CCGAGTCACT CGCCCCCGAG ATCCCCAAAC TCATCGTGTC AGGGGAGACC 
ATTGACGCGG TGAAGCAGAG CGCCTGCCTG TGTCTGCTGC GGCTCTTCCG CACATCGCCC 
GACACCGTGC CATCCGGCGA ATGGACGCAG AGGGTCGGCC ATCTTCTCAA CGACCAACAT 
CTGGGTGTAG TAACAGCTGC CACCAGTCTG ATCCACACTC TGGCACAGAA GACCCCGGAG 
GACTTCAAGA ATTGTGTTTC CCTGGCCATC TCTCGACTCA GTAGGATTGT GACCTCATCA 
TACACAGACC TCCAGGACTA CACCTACTAC TTTGTCCCTG CCCCCTGGCT GTCAGTAAAG 
CTACTCAGGC TGCTGCAAGT CTACCCACCA CCAGAGGACC CAGCGGCACG TGGTCGGCTG 
AACGAGTGTC TGGAGACCAT CCTGAACAAG GCCCAGGAAC CTCCCAAGTC TAAGAAGGTG 
CAGCACTCCA ACGCTAAGAA CGCTGTGCTG TTTGAGGCCA TCAACCTTAT TATTCACCAT 
GACACAGAAC CCACCCTGTT GGTGCGTGCG TGTAACCAGC TGGGCCAGTT CTTGCAGCAC 
AGAGAGACTA ACCTGCGGTA CCTGGCCCTG GAGAGCATGT GTCTGCTGGC CTCCTCCGAG 
TTCTCACACG AGGCAGTCAA GAAACACATG GACACGGTCA TCACCGCACT CAAGACTGAG 
CGTGACGTGA GCGTACGACA GCGAGCTGTA GATCTGCTGT ACGGGATGTG TGACAAGTCC 
AACGCTGAGG AGATCGTGGC CGAGATGCTG GCCTACCTGG AGACGGCCGA CTACTCCATC 
AGAGAGGAAA TGGTGTTGAA GGTGGCCATC TTGGCAGAAA AGTACCCCGT TGACTACACC 
TGGTACGTGG ACACCATCCT GAACCTGATC CGAATTGCTG GAGACTACGT CAGTGACGAG 
GTGTGGTTCC GTGTCATCCA GATTGTCATC AACAGGGACG ATGTGCAAGG GTATGCTGCA 
AAGGTCGTGT TTGAGGCACT CCAAGCGCCA GCCTGCCATG AGAACATGGT GAAAGTAGGA 
GGATATATCC TCGGGGAGTT CGGCAATCTC ATCGCAGGAG ACCAGAGATC CAGCCCGATG 
GTCCAGTTCC AGCTGCTGCA CTCCAAGTAC CACCTGTGCA GCGCCCCGAC CAGGGGACTT 
CTCCTCTCCA CATACATCAA GTTCATCAAC CTGTTCCCAG AGATCAAGGG CCAGCTCCAG 
GACGTGTTGC GGAATAACAA CAACCTGCGA AATGCGGACG CAGAGCTGCA GCAGAGAGCA 
ATCGAGTACC TACAGCTCAG TACGGTAGCC AGTCAGGACG TCCTGGCGAC AGTTCTAGAA 
GAAATGCCCC CCTTCCCTGA GAGAGAGTCG TCCATCCTGG CCAAGCTGAA GAAGAAGAAG 
CCCAACGTCT CAGACATCGA GGTGGTGAAG GAGGGCAAGA AGTCATCCAA CCCCCCCACC 
CTCAACAACA GCGCAGCCAA CACACCTGAG GCAACACCTG CATCAGCACC TGTACCTGTC 
ATGGCGGCTG CACCTGCTCC TGTCCAGAGT GCCTCTGATG ACCTGCTTGG TCTGAGCGCC 
GGTCCGGCCG CCGCTGCGCC CATTACCAAC AACTTCGCAG CCCCGGCGTC GTTCGCGGCT 
CCGGCGGCGC CCGCGGGAGG TGGCGGACTG CTCGTGGACG TGTTCGACAC GCCGCCTGCC 
CAGAACAACG TGAGCTCAGC GTTAGCGCCA CAAGCCGAGG AGAACTTCTT TAATGCGCCC 
GCCGGCGAAA CTGCGCCCTC CGGTGACACC CCTGCTTCGG AGCTTGACGG AAGGTTTGCG 
ATTAAGAACA ACGGTGTCCT GTTTGAGAAT GAACTCCTGC AGATCGGGGT CAAAGCAGAG 
TACAAGGGCA ACCTGGGACG CCTGGGCCTG TTCTATGGAA ACAAGACGTC GTACCCCTTC 
CAGTTCTTCA CTCCCACCGT GTCTTGTCCA GGAGAGCTGG CCTCTGCGTT GAATGTCCAG 
ACCAAACCCC TCGACGTGAA CACAGTGGAG GGAGGAGCGC AGGTCCAACA AATCATCAAC 
GTGGAGTGTA TATCAGACTT TGTGGACGCA CCCGTCCTCA GCATTAAGTT CAGTTACTCA 
GGCAGTATGC AGCAGATCAA CCTGATGCTG CCTGTCATGT TGAACAAGTT CTTTGAACCC 
GTGGAGATGG ACGCCACCAC TTTCTTCAAC AGGTGGAAAC AGCTCAACAG CCCTGGCCAG 
GAGGAGCAAA AGATTTTCAA GGCTACGTTC CCAATAGACT CGGATACCTC CAGGACAAAG 
CTTCTTGGCT TTGGGTTTGG ACTCCTGGAC GGGGTAGATC CGAACCCAGA CAACTTTGTC 
GGAGCAGGAA TCATCAAGGC ACAGTCCTCA CAGGTCGGCT GTCTGCTCAG ACTAGAGCCC 
AACATGCAAG CACAGATGTA CCGGTTAACT TTGCGAACCA GCAGCACGTC TGTCACCACA 
AGGCTGGCTG ATCTCCTTCT CAAGCTTTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109470275 ( XM_019769161.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019624720.1 Branchiostoma belcheri AP-2 complex subunit alpha-2-like (LOC109470275), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBr179609
Clone ID Related Accession (Same CDS sequence)	XM_019769161.1
Accession Version	XM_019769161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	AP-2 complex subunit alpha-2-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017803713.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAACG TGGCGAAGGG AGACGGGATG CGCGGCCTCG CCGTGTTCAT CTCCGACATC 
AGAAACTGTA AAAGCAAAGA AGCAGAGCAG AAGCGGATAA ACAAGGAACT GGCAAACATT 
AGATCCAAAT TCAAAGGTGA TAAGACCCTG GATGGATACA GTAAGAAGAA GTATGTGTGT 
AAACTGCTCT TCATTTTCCT GCTGGGACAT GACATTGACT TTGGGCACAT GGAGGCCGTC 
AACCTCCTGT CTTCCAACAA GTACACCGAG AAACAGATCG GTTATCTGTT TATCTCCGTG 
CTGGTCAACA CCAACAGTGA CCTGATCCGT CTGATTATCG GCTCCATCAA GAACGACCTC 
CTGTCCCGTA ACGCTGTCCA CATCTGCCTG GCCCTGCAGT GTATCGCCAA CATCGGCAGT 
AAGGAGATGG CCGAGTCACT CGCCCCCGAG ATCCCCAAAC TCATCGTGTC AGGGGAGACC 
ATTGACGCGG TGAAGCAGAG CGCCTGCCTG TGTCTGCTGC GGCTCTTCCG CACATCGCCC 
GACACCGTGC CATCCGGCGA ATGGACGCAG AGGGTCGGCC ATCTTCTCAA CGACCAACAT 
CTGGGTGTAG TAACAGCTGC CACCAGTCTG ATCCACACTC TGGCACAGAA GACCCCGGAG 
GACTTCAAGA ATTGTGTTTC CCTGGCCATC TCTCGACTCA GTAGGATTGT GACCTCATCA 
TACACAGACC TCCAGGACTA CACCTACTAC TTTGTCCCTG CCCCCTGGCT GTCAGTAAAG 
CTACTCAGGC TGCTGCAAGT CTACCCACCA CCAGAGGACC CAGCGGCACG TGGTCGGCTG 
AACGAGTGTC TGGAGACCAT CCTGAACAAG GCCCAGGAAC CTCCCAAGTC TAAGAAGGTG 
CAGCACTCCA ACGCTAAGAA CGCTGTGCTG TTTGAGGCCA TCAACCTTAT TATTCACCAT 
GACACAGAAC CCACCCTGTT GGTGCGTGCG TGTAACCAGC TGGGCCAGTT CTTGCAGCAC 
AGAGAGACTA ACCTGCGGTA CCTGGCCCTG GAGAGCATGT GTCTGCTGGC CTCCTCCGAG 
TTCTCACACG AGGCAGTCAA GAAACACATG GACACGGTCA TCACCGCACT CAAGACTGAG 
CGTGACGTGA GCGTACGACA GCGAGCTGTA GATCTGCTGT ACGGGATGTG TGACAAGTCC 
AACGCTGAGG AGATCGTGGC CGAGATGCTG GCCTACCTGG AGACGGCCGA CTACTCCATC 
AGAGAGGAAA TGGTGTTGAA GGTGGCCATC TTGGCAGAAA AGTACCCCGT TGACTACACC 
TGGTACGTGG ACACCATCCT GAACCTGATC CGAATTGCTG GAGACTACGT CAGTGACGAG 
GTGTGGTTCC GTGTCATCCA GATTGTCATC AACAGGGACG ATGTGCAAGG GTATGCTGCA 
AAGGTCGTGT TTGAGGCACT CCAAGCGCCA GCCTGCCATG AGAACATGGT GAAAGTAGGA 
GGATATATCC TCGGGGAGTT CGGCAATCTC ATCGCAGGAG ACCAGAGATC CAGCCCGATG 
GTCCAGTTCC AGCTGCTGCA CTCCAAGTAC CACCTGTGCA GCGCCCCGAC CAGGGGACTT 
CTCCTCTCCA CATACATCAA GTTCATCAAC CTGTTCCCAG AGATCAAGGG CCAGCTCCAG 
GACGTGTTGC GGAATAACAA CAACCTGCGA AATGCGGACG CAGAGCTGCA GCAGAGAGCA 
ATCGAGTACC TACAGCTCAG TACGGTAGCC AGTCAGGACG TCCTGGCGAC AGTTCTAGAA 
GAAATGCCCC CCTTCCCTGA GAGAGAGTCG TCCATCCTGG CCAAGCTGAA GAAGAAGAAG 
CCCAACGTCT CAGACATCGA GGTGGTGAAG GAGGGCAAGA AGTCATCCAA CCCCCCCACC 
CTCAACAACA GCGCAGCCAA CACACCTGAG GCAACACCTG CATCAGCACC TGTACCTGTC 
ATGGCGGCTG CACCTGCTCC TGTCCAGAGT GCCTCTGATG ACCTGCTTGG TCTGAGCGCC 
GGTCCGGCCG CCGCTGCGCC CATTACCAAC AACTTCGCAG CCCCGGCGTC GTTCGCGGCT 
CCGGCGGCGC CCGCGGGAGG TGGCGGACTG CTCGTGGACG TGTTCGACAC GCCGCCTGCC 
CAGAACAACG TGAGCTCAGC GTTAGCGCCA CAAGCCGAGG AGAACTTCTT TAAGTTTGCG 
ATTAAGAACA ACGGTGTCCT GTTTGAGAAT GAACTCCTGC AGATCGGGGT CAAAGCAGAG 
TACAAGGGCA ACCTGGGACG CCTGGGCCTG TTCTATGGAA ACAAGACGTC GTACCCCTTC 
CAGTTCTTCA CTCCCACCGT GTCTTGTCCA GGAGAGCTGG CCTCTGCGTT GAATGTCCAG 
ACCAAACCCC TCGACGTGAA CACAGTGGAG GGAGGAGCGC AGGTCCAACA AATCATCAAC 
GTGGAGTGTA TATCAGACTT TGTGGACGCA CCCGTCCTCA GCATTAAGTT CAGTTACTCA 
GGCAGTATGC AGCAGATCAA CCTGATGCTG CCTGTCATGT TGAACAAGTT CTTTGAACCC 
GTGGAGATGG ACGCCACCAC TTTCTTCAAC AGGTGGAAAC AGCTCAACAG CCCTGGCCAG 
GAGGAGCAAA AGATTTTCAA GGCTACGTTC CCAATAGACT CGGATACCTC CAGGACAAAG 
CTTCTTGGCT TTGGGTTTGG ACTCCTGGAC GGGGTAGATC CGAACCCAGA CAACTTTGTC 
GGAGCAGGAA TCATCAAGGC ACAGTCCTCA CAGGTCGGCT GTCTGCTCAG ACTAGAGCCC 
AACATGCAAG CACAGATGTA CCGGTTAACT TTGCGAACCA GCAGCACGTC TGTCACCACA 
AGGCTGGCTG ATCTCCTTCT CAAGCTTTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019624720.1
CDS	281..3193
Translation	MPNVAKGDGMRGLAVFISDIRNCKSKEAEQKRINKELANIRSKFKGDKTLDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNTNSDLIRLIIGSIKNDLLSRNAVHICLALQCIANIGSKEMAESLAPEIPKLIVSGETIDAVKQSACLCLLRLFRTSPDTVPSGEWTQRVGHLLNDQHLGVVTAATSLIHTLAQKTPEDFKNCVSLAISRLSRIVTSSYTDLQDYTYYFVPAPWLSVKLLRLLQVYPPPEDPAARGRLNECLETILNKAQEPPKSKKVQHSNAKNAVLFEAINLIIHHDTEPTLLVRACNQLGQFLQHRETNLRYLALESMCLLASSEFSHEAVKKHMDTVITALKTERDVSVRQRAVDLLYGMCDKSNAEEIVAEMLAYLETADYSIREEMVLKVAILAEKYPVDYTWYVDTILNLIRIAGDYVSDEVWFRVIQIVINRDDVQGYAAKVVFEALQAPACHENMVKVGGYILGEFGNLIAGDQRSSPMVQFQLLHSKYHLCSAPTRGLLLSTYIKFINLFPEIKGQLQDVLRNNNNLRNADAELQQRAIEYLQLSTVASQDVLATVLEEMPPFPERESSILAKLKKKKPNVSDIEVVKEGKKSSNPPTLNNSAANTPEATPASAPVPVMAAAPAPVQSASDDLLGLSAGPAAAAPITNNFAAPASFAAPAAPAGGGGLLVDVFDTPPAQNNVSSALAPQAEENFFKFAIKNNGVLFENELLQIGVKAEYKGNLGRLGLFYGNKTSYPFQFFTPTVSCPGELASALNVQTKPLDVNTVEGGAQVQQIINVECISDFVDAPVLSIKFSYSGSMQQINLMLPVMLNKFFEPVEMDATTFFNRWKQLNSPGQEEQKIFKATFPIDSDTSRTKLLGFGFGLLDGVDPNPDNFVGAGIIKAQSSQVGCLLRLEPNMQAQMYRLTLRTSSTSVTTRLADLLLKLF

Target ORF information:

RefSeq Version	XM_019769161.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri AP-2 complex subunit alpha-2-like (LOC109470275), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019769161.1

ORF Insert Sequence:

ATGCCCAACG TGGCGAAGGG AGACGGGATG CGCGGCCTCG CCGTGTTCAT CTCCGACATC 
AGAAACTGTA AAAGCAAAGA AGCAGAGCAG AAGCGGATAA ACAAGGAACT GGCAAACATT 
AGATCCAAAT TCAAAGGTGA TAAGACCCTG GATGGATACA GTAAGAAGAA GTATGTGTGT 
AAACTGCTCT TCATTTTCCT GCTGGGACAT GACATTGACT TTGGGCACAT GGAGGCCGTC 
AACCTCCTGT CTTCCAACAA GTACACCGAG AAACAGATCG GTTATCTGTT TATCTCCGTG 
CTGGTCAACA CCAACAGTGA CCTGATCCGT CTGATTATCG GCTCCATCAA GAACGACCTC 
CTGTCCCGTA ACGCTGTCCA CATCTGCCTG GCCCTGCAGT GTATCGCCAA CATCGGCAGT 
AAGGAGATGG CCGAGTCACT CGCCCCCGAG ATCCCCAAAC TCATCGTGTC AGGGGAGACC 
ATTGACGCGG TGAAGCAGAG CGCCTGCCTG TGTCTGCTGC GGCTCTTCCG CACATCGCCC 
GACACCGTGC CATCCGGCGA ATGGACGCAG AGGGTCGGCC ATCTTCTCAA CGACCAACAT 
CTGGGTGTAG TAACAGCTGC CACCAGTCTG ATCCACACTC TGGCACAGAA GACCCCGGAG 
GACTTCAAGA ATTGTGTTTC CCTGGCCATC TCTCGACTCA GTAGGATTGT GACCTCATCA 
TACACAGACC TCCAGGACTA CACCTACTAC TTTGTCCCTG CCCCCTGGCT GTCAGTAAAG 
CTACTCAGGC TGCTGCAAGT CTACCCACCA CCAGAGGACC CAGCGGCACG TGGTCGGCTG 
AACGAGTGTC TGGAGACCAT CCTGAACAAG GCCCAGGAAC CTCCCAAGTC TAAGAAGGTG 
CAGCACTCCA ACGCTAAGAA CGCTGTGCTG TTTGAGGCCA TCAACCTTAT TATTCACCAT 
GACACAGAAC CCACCCTGTT GGTGCGTGCG TGTAACCAGC TGGGCCAGTT CTTGCAGCAC 
AGAGAGACTA ACCTGCGGTA CCTGGCCCTG GAGAGCATGT GTCTGCTGGC CTCCTCCGAG 
TTCTCACACG AGGCAGTCAA GAAACACATG GACACGGTCA TCACCGCACT CAAGACTGAG 
CGTGACGTGA GCGTACGACA GCGAGCTGTA GATCTGCTGT ACGGGATGTG TGACAAGTCC 
AACGCTGAGG AGATCGTGGC CGAGATGCTG GCCTACCTGG AGACGGCCGA CTACTCCATC 
AGAGAGGAAA TGGTGTTGAA GGTGGCCATC TTGGCAGAAA AGTACCCCGT TGACTACACC 
TGGTACGTGG ACACCATCCT GAACCTGATC CGAATTGCTG GAGACTACGT CAGTGACGAG 
GTGTGGTTCC GTGTCATCCA GATTGTCATC AACAGGGACG ATGTGCAAGG GTATGCTGCA 
AAGGTCGTGT TTGAGGCACT CCAAGCGCCA GCCTGCCATG AGAACATGGT GAAAGTAGGA 
GGATATATCC TCGGGGAGTT CGGCAATCTC ATCGCAGGAG ACCAGAGATC CAGCCCGATG 
GTCCAGTTCC AGCTGCTGCA CTCCAAGTAC CACCTGTGCA GCGCCCCGAC CAGGGGACTT 
CTCCTCTCCA CATACATCAA GTTCATCAAC CTGTTCCCAG AGATCAAGGG CCAGCTCCAG 
GACGTGTTGC GGAATAACAA CAACCTGCGA AATGCGGACG CAGAGCTGCA GCAGAGAGCA 
ATCGAGTACC TACAGCTCAG TACGGTAGCC AGTCAGGACG TCCTGGCGAC AGTTCTAGAA 
GAAATGCCCC CCTTCCCTGA GAGAGAGTCG TCCATCCTGG CCAAGCTGAA GAAGAAGAAG 
CCCAACGTCT CAGACATCGA GGTGGTGAAG GAGGGCAAGA AGTCATCCAA CCCCCCCACC 
CTCAACAACA GCGCAGCCAA CACACCTGAG GCAACACCTG CATCAGCACC TGTACCTGTC 
ATGGCGGCTG CACCTGCTCC TGTCCAGAGT GCCTCTGATG ACCTGCTTGG TCTGAGCGCC 
GGTCCGGCCG CCGCTGCGCC CATTACCAAC AACTTCGCAG CCCCGGCGTC GTTCGCGGCT 
CCGGCGGCGC CCGCGGGAGG TGGCGGACTG CTCGTGGACG TGTTCGACAC GCCGCCTGCC 
CAGAACAACG TGAGCTCAGC GTTAGCGCCA CAAGCCGAGG AGAACTTCTT TAAGTTTGCG 
ATTAAGAACA ACGGTGTCCT GTTTGAGAAT GAACTCCTGC AGATCGGGGT CAAAGCAGAG 
TACAAGGGCA ACCTGGGACG CCTGGGCCTG TTCTATGGAA ACAAGACGTC GTACCCCTTC 
CAGTTCTTCA CTCCCACCGT GTCTTGTCCA GGAGAGCTGG CCTCTGCGTT GAATGTCCAG 
ACCAAACCCC TCGACGTGAA CACAGTGGAG GGAGGAGCGC AGGTCCAACA AATCATCAAC 
GTGGAGTGTA TATCAGACTT TGTGGACGCA CCCGTCCTCA GCATTAAGTT CAGTTACTCA 
GGCAGTATGC AGCAGATCAA CCTGATGCTG CCTGTCATGT TGAACAAGTT CTTTGAACCC 
GTGGAGATGG ACGCCACCAC TTTCTTCAAC AGGTGGAAAC AGCTCAACAG CCCTGGCCAG 
GAGGAGCAAA AGATTTTCAA GGCTACGTTC CCAATAGACT CGGATACCTC CAGGACAAAG 
CTTCTTGGCT TTGGGTTTGG ACTCCTGGAC GGGGTAGATC CGAACCCAGA CAACTTTGTC 
GGAGCAGGAA TCATCAAGGC ACAGTCCTCA CAGGTCGGCT GTCTGCTCAG ACTAGAGCCC 
AACATGCAAG CACAGATGTA CCGGTTAACT TTGCGAACCA GCAGCACGTC TGTCACCACA 
AGGCTGGCTG ATCTCCTTCT CAAGCTTTTC TGA