RAPGEF6 cDNA ORF clone, Bos taurus(cattle)

The following RAPGEF6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236589 XM_005209079.4
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236590 XM_024995048.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236591 XM_024995049.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236592 XM_024995050.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236581 XM_003586275.5
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236582 XM_002688965.6
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236583 XM_024995044.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236584 XM_024995045.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236585 XM_015472120.2
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236586 XM_005209077.4
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236587 XM_024995046.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236588 XM_024995047.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa90291 XM_015472120.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa90291 XM_015464187.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa21248 XM_003586275.4
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa21248 XM_015464185.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa21249 XM_015464186.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa21249 XM_002688965.5
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa21251 XM_005209077.3
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa21251 XM_015464188.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa21253 XM_015464189.1
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21253 XM_005209079.3
Latest version!
Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236589
    Clone ID Related Accession (Same CDS sequence) XM_005209079.4
    Accession Version XM_005209079.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209079.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209136.2
    CDS98..4483
    Translation

    Target ORF information:

    RefSeq Version XM_005209079.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209079.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236590
    Clone ID Related Accession (Same CDS sequence) XM_024995048.1
    Accession Version XM_024995048.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGTTGGC
    AGTATCCTAT CTGATCACAG CATCCTGCTG CGCCTCTCAG GGTCTTCCTC ATCCCCTCCC
    CGTGAGTCCA GCACCAACAT CAGTGGCCAG AGCTGCCCCG GAATAGGTGG GGTTTACTTG
    CAAAAGAAAA TCCTTCAGAT TACCAGGAGC ACAGCCAAAA GAACAGACAG GACCGAGAAG
    GCCACTGAGG AGAATAGAGA CAGGACAAGC TGTGACAACA CAGCCAGAAA AAGAATGACA
    TCCCCGTTTA GAAGATTTAG GGAAAGAACT CTTTCAAGGG AGAGACTAGC CAACAGCAAA
    AAAGAGGACG CAGATCACAA TCAAGCTACA GAAAGCTGTG AGAAAGTGAA GGATGTTGGA
    AGCAACATCA AAGATGAGAA AGGGAGTGAC ATCTTCAATT CAAATAGCCG AGGCAACAGT
    AACACTTTAC ACTGCTTCTA TACGCGATTC ACGTCTAAAA GGAGAAAGAC ACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850816.1
    CDS193..4449
    Translation

    Target ORF information:

    RefSeq Version XM_024995048.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995048.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGTTGGC
    AGTATCCTAT CTGATCACAG CATCCTGCTG CGCCTCTCAG GGTCTTCCTC ATCCCCTCCC
    CGTGAGTCCA GCACCAACAT CAGTGGCCAG AGCTGCCCCG GAATAGGTGG GGTTTACTTG
    CAAAAGAAAA TCCTTCAGAT TACCAGGAGC ACAGCCAAAA GAACAGACAG GACCGAGAAG
    GCCACTGAGG AGAATAGAGA CAGGACAAGC TGTGACAACA CAGCCAGAAA AAGAATGACA
    TCCCCGTTTA GAAGATTTAG GGAAAGAACT CTTTCAAGGG AGAGACTAGC CAACAGCAAA
    AAAGAGGACG CAGATCACAA TCAAGCTACA GAAAGCTGTG AGAAAGTGAA GGATGTTGGA
    AGCAACATCA AAGATGAGAA AGGGAGTGAC ATCTTCAATT CAAATAGCCG AGGCAACAGT
    AACACTTTAC ACTGCTTCTA TACGCGATTC ACGTCTAAAA GGAGAAAGAC ACTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236591
    Clone ID Related Accession (Same CDS sequence) XM_024995049.1
    Accession Version XM_024995049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4233bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GTTGGCAGTA TCCTATCTGA TCACAGCATC
    CTGCTGCGCC TCTCAGGGTC TTCCTCATCC CCTCCCCGTG AGTCCAGCAC CAACATCAGT
    GGCCAGAGCT GCCCCGGAAT AGGTGGGGTT TACTTGCAAA AGAAAATCCT TCAGATTACC
    AGGAGCACAG CCAAAAGAAC AGACAGGACC GAGAAGGCCA CTGAGGAGAA TAGAGACAGG
    ACAAGCTGTG ACAACACAGC CAGAAAAAGA ATGACATCCC CGTTTAGAAG ATTTAGGGAA
    AGAACTCTTT CAAGGGAGAG ACTAGCCAAC AGCAAAAAAG AGGACGCAGA TCACAATCAA
    GCTACAGAAA GCTGTGAGAA AGTGAAGGAT GTTGGAAGCA ACATCAAAGA TGAGAAAGGG
    AGTGACATCT TCAATTCAAA TAGCCGAGGC AACAGTAACA CTTTACACTG CTTCTATACG
    CGATTCACGT CTAAAAGGAG AAAGACACTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850817.1
    CDS193..4425
    Translation

    Target ORF information:

    RefSeq Version XM_024995049.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GTTGGCAGTA TCCTATCTGA TCACAGCATC
    CTGCTGCGCC TCTCAGGGTC TTCCTCATCC CCTCCCCGTG AGTCCAGCAC CAACATCAGT
    GGCCAGAGCT GCCCCGGAAT AGGTGGGGTT TACTTGCAAA AGAAAATCCT TCAGATTACC
    AGGAGCACAG CCAAAAGAAC AGACAGGACC GAGAAGGCCA CTGAGGAGAA TAGAGACAGG
    ACAAGCTGTG ACAACACAGC CAGAAAAAGA ATGACATCCC CGTTTAGAAG ATTTAGGGAA
    AGAACTCTTT CAAGGGAGAG ACTAGCCAAC AGCAAAAAAG AGGACGCAGA TCACAATCAA
    GCTACAGAAA GCTGTGAGAA AGTGAAGGAT GTTGGAAGCA ACATCAAAGA TGAGAAAGGG
    AGTGACATCT TCAATTCAAA TAGCCGAGGC AACAGTAACA CTTTACACTG CTTCTATACG
    CGATTCACGT CTAAAAGGAG AAAGACACTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236592
    Clone ID Related Accession (Same CDS sequence) XM_024995050.1
    Accession Version XM_024995050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATTGTGG TAGAGAATTC CAAAGATAAT GAAGATAATA TTCTACAAAG AGAAATTCCT 
    GCCAGACAGT CTCGAAGAAG ATTTCGGAAA ATTAATTGTA AAGGAGAACG CCAGACTATT
    ACTGATGATG TGGATATTAA CAGCTATCTT TCTCTTCCAG CCGATCTTAC CAAGATGCAC
    ATTGCAGACA ACCCTCATCC ACAGGTGACT CACGTGTCTT CTAGTCAGTC TGGCTGTAGC
    ATCGCCAGTG ACTCTGGAAG CAGCAGTTTA TCTGATATCT ATCAGGCTAC CGAAAGTGAG
    GTAGGAGATG TCGATTTAAC ACGTCTTCCA GAAGGACCTG TTGATTCTGA AGATGAAGAA
    GAGGAAGATG AAGACATTGA TCGAACAGAT CCTCTGCAAG GGCGGGATCT TGTCCGAGAA
    TGTCTTGAAA AAGAACCTGC AGATAAAACT GATGATGACA TTGAACAATT GCTGGAGTTT
    ATGCACCAGC TTCCCGCTTT TGCAAATATG ACCATGTCTG TAAGGAGAGA ACTCTGCTCA
    GTGATGATTT TTGAAGTGGT AGAGCAGGCT GGAGCTGTCA TTCTTGAAGA TGGGCAAGAG
    CTTGATTCAT GGTATGTTAT TTTAAATGGC ACTGTGGAAA TCAGTCATCC AGATGGAAAA
    GTTGACAATC TCTTTATGGG AAATAGTTTT GGAATCACAC CCACTCTGGA TAAACAGTAC
    ATGCATGGAG TTGTTAGGAC TAAAGTAGAT GACTGTCAGT TTGTCTGCAT AGCCCAGCAG
    GATTATTGGA GAATTTTAAA CCATGTTGAA AAAAATACCC ATAAAGTTGA GGAAGAGGGA
    GAAATTGTTA TGGTACATGA GCATCGTGAA CTGGATCGAA GTGGAACCAG GAAAGGACAC
    ATTGTAATTA AGGGAACACC TGAGCGTCTC ATCATGCATT TAATAGAAGA GCATTCCATT
    GTGGACCCAA CTTATATAGA AGATTTCCTA TTAACTTACA GAACATTTCT TGCAAGTCCT
    TTGGATGTTG GGACCAAACT GTTGGAATGG TTTAAAATTG ACAGCTTAAG GGATAAGGTA
    ACACGGATTG TATTATTATG GGTAAATAAT CATTTCAATG ATTTTGAAGG TGATCCTGCT
    ATGACTCGAT TTCTAGAGGA ATTTGAAAAA AATCTGGAAG ATACAAAAAT GAATGGTCAT
    CTCCGGTTAT TGAATATTGC GTGTGCTGCA AAGGCTAAGT GGAGACAGGT TGTGCTGCAG
    AAAGCTTCCC GGGAGTCACC TCTGCATTTC AGCCTAAGTG GAGGAAGTGA GAAGGGATTT
    GGTATTTTTG TTGAAAGCGT AGAACCTAGT AGCAAAGCTG CTGATGCAGG ACTGAAACGT
    GGTGATCAAG TAATGGAAGT AAATGGACAA AATTTTGAGA ATATAACGTT TGTGAAGGCC
    CTTGAAATTT TGAGGAATAA TACTCATCTT GCACTTACTG TAAAGACCAA CATTTTTGTG
    TTCAAAGAGT TACTTAGTAG GACTGAACAA GAGAAATCTG GTGTTCCTCA TATTCCCAAA
    ATTGCTGAAA AAAAAAGTAA TCGCCACTCA ATCCAGGATG TACCAGGAGA TCTTGAACAG
    ACATCACAGG AGAAAGGAAA TAAGAAAATA AAAGCAAATA CCGTTTCAGG TGGAAGAAAC
    AAAATCAGGA AAATTTTGGA TAAAACACGA TTTAGTATCT TGCCTCCAAA ACTTTTTAGT
    GATGGAGGCC TGAGCCAGTC ACAGGATGAC AGCATTGTGG GAACGAGACA CTGCAGGCAC
    AGTCTGGCCA TCATGCCTAT CCCTGGAACA CTCTCGTCCA GCAGCCCCGA CCTCCTCCAG
    CCTACCACCA GCATGTTGGA TTTTTCTAAT CCCTCAGATA TTCCTGATCA AGTTATAAGA
    GTTTTCAAAG CAGATCAGCA AAGTTGCTAC ATTATCATCA GTAAAGACAC TACAGCTAAG
    GAAGTTGTCT GTCAAGCTAT TCATGAATTT GGTTTGACTG GTGCATCTGA CACATACTCT
    CTCTGTGAAG TTTCTGTTAC TCCTGAGGGT GTTATAAAAC AGAGAAGACT TCCAGACCAA
    TTCTCCAAAT TAGCTGATAG AATTCAGCTC AATGGAAGGT ATTATTTGAA AAATAACATG
    GAAACAGAAA CCCTATGTTC AGATGAAGAT GCTCAAGAAC TAGTTAAGGA AAGCCAGCTG
    TCCATGCTAC AACTAAGTAC TATTGAGGTG GCCACTCAGC TGTCAATGAG GGACTTTGAT
    TTATTTCGTA ATATTGAGCC TACTGAATAT ATTGATGACC TTTTTAAGTT AGAGTCCAAA
    ACAGGAAATA CTCACTTGAA AGAATTTGAA GACATTGTAA ACCAAGAGAC ATTCTGGGTT
    GCATCAGAGA TTTTAACTGA ATCAAATCAG CTCAAACGAA TGAAGATTAT TAAACATTTT
    ATTAAAATTG CACTTCATTG TCGAGAATGT AAGAACTTCA ATTCCATGTT TGCAATAATA
    AGTGGTTTGA ACTTGGCATC TGTAGCACGA CTCAGAGGTA CTTGGGAAAA GTTACCAAGC
    AAGTATGATA AACACTTCCA AGATCTGCAA GACCTTTTTG ATCCATCCAG AAACATGGCA
    AAATACAGAA ATATTCTTAG TAGTCAAAGC ATGCAGCCTC CAATTATTCC ACTCTTTCCT
    GTTGTCAAGA AAGATATGAC ATTTCTACAT GAAGGAAATG ATTCCAAAGT TGATGGTTTG
    GTAAATTTCG AGAAACTCAG AATGATTGCC AAGGAAATTC GTCAGGTTGT TCGAATGACT
    TCTGCTAACA TGGATCCAGC TATGATGTTC CGACAGAGGA AGAAGAGGTG GCGGAGTCTG
    GGGTCACTGA GTCAAGGCAG CACAAATTCA AACATGCTGG ATGTTCAGGG AGGTGCTCAC
    AAAAAAAGGG CACGCCGTAG CTCTCTGCTA AATGCCAAGA AGCTATATGA AGATGCCCAA
    ATGGCAAGAA AAGTGAAGCA GTATCTTTCC AGTCTGGATG TCGAGACAGA TGAAGAGAAG
    TTCCAGATGA TGTCATTGCA ATGGGAGCCT GCATATGGTA CCTTGACCAA GAATTTAAAT
    GAGAAAAAAT CAATGAAGTC ATCAGAAATG TCTCCGGTGC CACTGAGGTC AGCTGGCCAA
    ACAACTAAAG CTCATTTGCA TCAGCCTCAC AGAGGAAGCC AGGTGCTTCA GGTGCCAGCT
    GTAAATTTGC ACCCTATCAG GAAGAAGGGC CAAGGAAAAG ACCCTGCCAC CCTGAGTACA
    GGTTTACCTC AGAAAATTTT AGGAACAGCT GAAGAAGTAA GTGGTGGTAA GAAACACACA
    GAAGACACCG TTTCTGTGGC ATCATCCTTA CATTCCAGCC CTCCTGTGTC TCCTCAAGGT
    TCCCCCCGCA AAGGTTATAC ACTTATACCA TCAGCTAAAT CTGACAACTT GTCTGACTCC
    AGCCACAGTG AGATTTCTTC ACGGTCCAGC ATTGTGAGCA ATTGTTCTGT TGACTCCATG
    TCTACAGCTC TACAGGATGA ACGGTGTTCC TCCCAGGCTG TGGCAGTCCC TGAATCCACT
    GGAGCATTGG AAAAGACAGA GCACTCCTCA GGGATAGGAG ACCATAGTCA GCTTGGCCCT
    GGGTGGACAC TCTCAAAGCC ATCTCTAATC AACAAGAGTT TAGCTGTCTC ATCTTCTGTG
    AGCAATGAAG AGATTTCTCA TGAGCACATC ATTATAGAAA CAGCTGATAG TGGTCGTGGA
    AGTTGGACTT CCTGCTCAAG CAGTTCCCAT GACAACTTCC AAAGTCTTCC AAACCCCAAA
    GGCTGGGAGT TTTTGAACTC TTACCGACCT ACCCATTTGG ATGGTCCCAT AGCTGAAGTT
    GAACCCACTG ACTGTGAGCA CTACAGCTGT CCCAAAGGCT GCTCTCGAAC TTGTGGTCAG
    TGTAAAGGAA GCCTAGAGAC TAATCAGATG AGACAGAGTT GGGCTTCCTC TAGTTCTCTG
    TCTGACACAT ACGAACCAAA CTATGGGACA GTTAAACGGA GAGTGCTGGA GAACACCCCA
    GCTGAATCAT CTGAAGGCTT GGACCACAAA GAGGGCATCG ACCCAGTCTA TAAAACTGTT
    ACTTCAAGTA CAGAAAAGGG CTTGATTGTG TACTGTGTCA CCTCACCCAA GAAGGAGGAT
    AGGTATAGGG AGCCACCTCC CACTCCTCCA GGATACATGG GGATTTCTTT AGCGGACCTA
    AAGGAAGGAC CCCACCTCCA CCTAAAACCT CCAGAGTATA GTGTGGCGGT GCAGAGGTCA
    AAGATGATGC ATAACAGCCT CTCTAGACTG CCACCAGCTT CTCTCAGTAG CAGCCCTGTG
    GCCTGTGTTC CATCGAAGAT TGTAATTCAG CCTCAGAGGC ATAAACTAGA TGTGACTGAT
    GGAGATAGCG AAGCAGATGA AAATGAACAG GTTTCAGCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850818.1
    CDS124..4608
    Translation

    Target ORF information:

    RefSeq Version XM_024995050.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATTGTGG TAGAGAATTC CAAAGATAAT GAAGATAATA TTCTACAAAG AGAAATTCCT 
    GCCAGACAGT CTCGAAGAAG ATTTCGGAAA ATTAATTGTA AAGGAGAACG CCAGACTATT
    ACTGATGATG TGGATATTAA CAGCTATCTT TCTCTTCCAG CCGATCTTAC CAAGATGCAC
    ATTGCAGACA ACCCTCATCC ACAGGTGACT CACGTGTCTT CTAGTCAGTC TGGCTGTAGC
    ATCGCCAGTG ACTCTGGAAG CAGCAGTTTA TCTGATATCT ATCAGGCTAC CGAAAGTGAG
    GTAGGAGATG TCGATTTAAC ACGTCTTCCA GAAGGACCTG TTGATTCTGA AGATGAAGAA
    GAGGAAGATG AAGACATTGA TCGAACAGAT CCTCTGCAAG GGCGGGATCT TGTCCGAGAA
    TGTCTTGAAA AAGAACCTGC AGATAAAACT GATGATGACA TTGAACAATT GCTGGAGTTT
    ATGCACCAGC TTCCCGCTTT TGCAAATATG ACCATGTCTG TAAGGAGAGA ACTCTGCTCA
    GTGATGATTT TTGAAGTGGT AGAGCAGGCT GGAGCTGTCA TTCTTGAAGA TGGGCAAGAG
    CTTGATTCAT GGTATGTTAT TTTAAATGGC ACTGTGGAAA TCAGTCATCC AGATGGAAAA
    GTTGACAATC TCTTTATGGG AAATAGTTTT GGAATCACAC CCACTCTGGA TAAACAGTAC
    ATGCATGGAG TTGTTAGGAC TAAAGTAGAT GACTGTCAGT TTGTCTGCAT AGCCCAGCAG
    GATTATTGGA GAATTTTAAA CCATGTTGAA AAAAATACCC ATAAAGTTGA GGAAGAGGGA
    GAAATTGTTA TGGTACATGA GCATCGTGAA CTGGATCGAA GTGGAACCAG GAAAGGACAC
    ATTGTAATTA AGGGAACACC TGAGCGTCTC ATCATGCATT TAATAGAAGA GCATTCCATT
    GTGGACCCAA CTTATATAGA AGATTTCCTA TTAACTTACA GAACATTTCT TGCAAGTCCT
    TTGGATGTTG GGACCAAACT GTTGGAATGG TTTAAAATTG ACAGCTTAAG GGATAAGGTA
    ACACGGATTG TATTATTATG GGTAAATAAT CATTTCAATG ATTTTGAAGG TGATCCTGCT
    ATGACTCGAT TTCTAGAGGA ATTTGAAAAA AATCTGGAAG ATACAAAAAT GAATGGTCAT
    CTCCGGTTAT TGAATATTGC GTGTGCTGCA AAGGCTAAGT GGAGACAGGT TGTGCTGCAG
    AAAGCTTCCC GGGAGTCACC TCTGCATTTC AGCCTAAGTG GAGGAAGTGA GAAGGGATTT
    GGTATTTTTG TTGAAAGCGT AGAACCTAGT AGCAAAGCTG CTGATGCAGG ACTGAAACGT
    GGTGATCAAG TAATGGAAGT AAATGGACAA AATTTTGAGA ATATAACGTT TGTGAAGGCC
    CTTGAAATTT TGAGGAATAA TACTCATCTT GCACTTACTG TAAAGACCAA CATTTTTGTG
    TTCAAAGAGT TACTTAGTAG GACTGAACAA GAGAAATCTG GTGTTCCTCA TATTCCCAAA
    ATTGCTGAAA AAAAAAGTAA TCGCCACTCA ATCCAGGATG TACCAGGAGA TCTTGAACAG
    ACATCACAGG AGAAAGGAAA TAAGAAAATA AAAGCAAATA CCGTTTCAGG TGGAAGAAAC
    AAAATCAGGA AAATTTTGGA TAAAACACGA TTTAGTATCT TGCCTCCAAA ACTTTTTAGT
    GATGGAGGCC TGAGCCAGTC ACAGGATGAC AGCATTGTGG GAACGAGACA CTGCAGGCAC
    AGTCTGGCCA TCATGCCTAT CCCTGGAACA CTCTCGTCCA GCAGCCCCGA CCTCCTCCAG
    CCTACCACCA GCATGTTGGA TTTTTCTAAT CCCTCAGATA TTCCTGATCA AGTTATAAGA
    GTTTTCAAAG CAGATCAGCA AAGTTGCTAC ATTATCATCA GTAAAGACAC TACAGCTAAG
    GAAGTTGTCT GTCAAGCTAT TCATGAATTT GGTTTGACTG GTGCATCTGA CACATACTCT
    CTCTGTGAAG TTTCTGTTAC TCCTGAGGGT GTTATAAAAC AGAGAAGACT TCCAGACCAA
    TTCTCCAAAT TAGCTGATAG AATTCAGCTC AATGGAAGGT ATTATTTGAA AAATAACATG
    GAAACAGAAA CCCTATGTTC AGATGAAGAT GCTCAAGAAC TAGTTAAGGA AAGCCAGCTG
    TCCATGCTAC AACTAAGTAC TATTGAGGTG GCCACTCAGC TGTCAATGAG GGACTTTGAT
    TTATTTCGTA ATATTGAGCC TACTGAATAT ATTGATGACC TTTTTAAGTT AGAGTCCAAA
    ACAGGAAATA CTCACTTGAA AGAATTTGAA GACATTGTAA ACCAAGAGAC ATTCTGGGTT
    GCATCAGAGA TTTTAACTGA ATCAAATCAG CTCAAACGAA TGAAGATTAT TAAACATTTT
    ATTAAAATTG CACTTCATTG TCGAGAATGT AAGAACTTCA ATTCCATGTT TGCAATAATA
    AGTGGTTTGA ACTTGGCATC TGTAGCACGA CTCAGAGGTA CTTGGGAAAA GTTACCAAGC
    AAGTATGATA AACACTTCCA AGATCTGCAA GACCTTTTTG ATCCATCCAG AAACATGGCA
    AAATACAGAA ATATTCTTAG TAGTCAAAGC ATGCAGCCTC CAATTATTCC ACTCTTTCCT
    GTTGTCAAGA AAGATATGAC ATTTCTACAT GAAGGAAATG ATTCCAAAGT TGATGGTTTG
    GTAAATTTCG AGAAACTCAG AATGATTGCC AAGGAAATTC GTCAGGTTGT TCGAATGACT
    TCTGCTAACA TGGATCCAGC TATGATGTTC CGACAGAGGA AGAAGAGGTG GCGGAGTCTG
    GGGTCACTGA GTCAAGGCAG CACAAATTCA AACATGCTGG ATGTTCAGGG AGGTGCTCAC
    AAAAAAAGGG CACGCCGTAG CTCTCTGCTA AATGCCAAGA AGCTATATGA AGATGCCCAA
    ATGGCAAGAA AAGTGAAGCA GTATCTTTCC AGTCTGGATG TCGAGACAGA TGAAGAGAAG
    TTCCAGATGA TGTCATTGCA ATGGGAGCCT GCATATGGTA CCTTGACCAA GAATTTAAAT
    GAGAAAAAAT CAATGAAGTC ATCAGAAATG TCTCCGGTGC CACTGAGGTC AGCTGGCCAA
    ACAACTAAAG CTCATTTGCA TCAGCCTCAC AGAGGAAGCC AGGTGCTTCA GGTGCCAGCT
    GTAAATTTGC ACCCTATCAG GAAGAAGGGC CAAGGAAAAG ACCCTGCCAC CCTGAGTACA
    GGTTTACCTC AGAAAATTTT AGGAACAGCT GAAGAAGTAA GTGGTGGTAA GAAACACACA
    GAAGACACCG TTTCTGTGGC ATCATCCTTA CATTCCAGCC CTCCTGTGTC TCCTCAAGGT
    TCCCCCCGCA AAGGTTATAC ACTTATACCA TCAGCTAAAT CTGACAACTT GTCTGACTCC
    AGCCACAGTG AGATTTCTTC ACGGTCCAGC ATTGTGAGCA ATTGTTCTGT TGACTCCATG
    TCTACAGCTC TACAGGATGA ACGGTGTTCC TCCCAGGCTG TGGCAGTCCC TGAATCCACT
    GGAGCATTGG AAAAGACAGA GCACTCCTCA GGGATAGGAG ACCATAGTCA GCTTGGCCCT
    GGGTGGACAC TCTCAAAGCC ATCTCTAATC AACAAGAGTT TAGCTGTCTC ATCTTCTGTG
    AGCAATGAAG AGATTTCTCA TGAGCACATC ATTATAGAAA CAGCTGATAG TGGTCGTGGA
    AGTTGGACTT CCTGCTCAAG CAGTTCCCAT GACAACTTCC AAAGTCTTCC AAACCCCAAA
    GGCTGGGAGT TTTTGAACTC TTACCGACCT ACCCATTTGG ATGGTCCCAT AGCTGAAGTT
    GAACCCACTG ACTGTGAGCA CTACAGCTGT CCCAAAGGCT GCTCTCGAAC TTGTGGTCAG
    TGTAAAGGAA GCCTAGAGAC TAATCAGATG AGACAGAGTT GGGCTTCCTC TAGTTCTCTG
    TCTGACACAT ACGAACCAAA CTATGGGACA GTTAAACGGA GAGTGCTGGA GAACACCCCA
    GCTGAATCAT CTGAAGGCTT GGACCACAAA GAGGGCATCG ACCCAGTCTA TAAAACTGTT
    ACTTCAAGTA CAGAAAAGGG CTTGATTGTG TACTGTGTCA CCTCACCCAA GAAGGAGGAT
    AGGTATAGGG AGCCACCTCC CACTCCTCCA GGATACATGG GGATTTCTTT AGCGGACCTA
    AAGGAAGGAC CCCACCTCCA CCTAAAACCT CCAGAGTATA GTGTGGCGGT GCAGAGGTCA
    AAGATGATGC ATAACAGCCT CTCTAGACTG CCACCAGCTT CTCTCAGTAG CAGCCCTGTG
    GCCTGTGTTC CATCGAAGAT TGTAATTCAG CCTCAGAGGC ATAAACTAGA TGTGACTGAT
    GGAGATAGCG AAGCAGATGA AAATGAACAG GTTTCAGCAG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236581
    Clone ID Related Accession (Same CDS sequence) XM_003586275.5
    Accession Version XM_003586275.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586275.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT
    CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC
    CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC
    CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG
    ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT
    GAAAATGAAC AGGTTTCAGC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586323.2
    CDS237..5063
    Translation

    Target ORF information:

    RefSeq Version XM_003586275.5
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586275.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT
    CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC
    CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC
    CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG
    ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT
    GAAAATGAAC AGGTTTCAGC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236582
    Clone ID Related Accession (Same CDS sequence) XM_002688965.6
    Accession Version XM_002688965.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002688965.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689011.3
    CDS211..5013
    Translation

    Target ORF information:

    RefSeq Version XM_002688965.6
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002688965.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236583
    Clone ID Related Accession (Same CDS sequence) XM_024995044.1
    Accession Version XM_024995044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT
    GTATTAGAGC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGATAATATT
    CTACAAAGAG AAATTCCTGC CAGACAGTCT CGAAGAAGAT TTCGGAAAAT TAATTGTAAA
    GGAGAACGCC AGACTATTAC TGATGATGTG GATATTAACA GCTATCTTTC TCTTCCAGCC
    GATCTTACCA AGATGCACAT TGCAGACAAC CCTCATCCAC AGGTGACTCA CGTGTCTTCT
    AGTCAGTCTG GCTGTAGCAT CGCCAGTGAC TCTGGAAGCA GCAGTTTATC TGATATCTAT
    CAGGCTACCG AAAGTGAGGT AGGAGATGTC GATTTAACAC GTCTTCCAGA AGGACCTGTT
    GATTCTGAAG ATGAAGAAGA GGAAGATGAA GACATTGATC GAACAGATCC TCTGCAAGGG
    CGGGATCTTG TCCGAGAATG TCTTGAAAAA GAACCTGCAG ATAAAACTGA TGATGACATT
    GAACAATTGC TGGAGTTTAT GCACCAGCTT CCCGCTTTTG CAAATATGAC CATGTCTGTA
    AGGAGAGAAC TCTGCTCAGT GATGATTTTT GAAGTGGTAG AGCAGGCTGG AGCTGTCATT
    CTTGAAGATG GGCAAGAGCT TGATTCATGG TATGTTATTT TAAATGGCAC TGTGGAAATC
    AGTCATCCAG ATGGAAAAGT TGACAATCTC TTTATGGGAA ATAGTTTTGG AATCACACCC
    ACTCTGGATA AACAGTACAT GCATGGAGTT GTTAGGACTA AAGTAGATGA CTGTCAGTTT
    GTCTGCATAG CCCAGCAGGA TTATTGGAGA ATTTTAAACC ATGTTGAAAA AAATACCCAT
    AAAGTTGAGG AAGAGGGAGA AATTGTTATG GTACATGAGC ATCGTGAACT GGATCGAAGT
    GGAACCAGGA AAGGACACAT TGTAATTAAG GGAACACCTG AGCGTCTCAT CATGCATTTA
    ATAGAAGAGC ATTCCATTGT GGACCCAACT TATATAGAAG ATTTCCTATT AACTTACAGA
    ACATTTCTTG CAAGTCCTTT GGATGTTGGG ACCAAACTGT TGGAATGGTT TAAAATTGAC
    AGCTTAAGGG ATAAGGTAAC ACGGATTGTA TTATTATGGG TAAATAATCA TTTCAATGAT
    TTTGAAGGTG ATCCTGCTAT GACTCGATTT CTAGAGGAAT TTGAAAAAAA TCTGGAAGAT
    ACAAAAATGA ATGGTCATCT CCGGTTATTG AATATTGCGT GTGCTGCAAA GGCTAAGTGG
    AGACAGGTTG TGCTGCAGAA AGCTTCCCGG GAGTCACCTC TGCATTTCAG CCTAAGTGGA
    GGAAGTGAGA AGGGATTTGG TATTTTTGTT GAAAGCGTAG AACCTAGTAG CAAAGCTGCT
    GATGCAGGAC TGAAACGTGG TGATCAAGTA ATGGAAGTAA ATGGACAAAA TTTTGAGAAT
    ATAACGTTTG TGAAGGCCCT TGAAATTTTG AGGAATAATA CTCATCTTGC ACTTACTGTA
    AAGACCAACA TTTTTGTGTT CAAAGAGTTA CTTAGTAGGA CTGAACAAGA GAAATCTGGT
    GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCACTCAAT CCAGGATGTA
    CCAGGAGATC TTGAACAGAC ATCACAGGAG AAAGGAAATA AGAAAATAAA AGCAAATACC
    GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG
    CCTCCAAAAC TTTTTAGTGA TGGAGGCCTG AGCCAGTCAC AGGATGACAG CATTGTGGGA
    ACGAGACACT GCAGGCACAG TCTGGCCATC ATGCCTATCC CTGGAACACT CTCGTCCAGC
    AGCCCCGACC TCCTCCAGCC TACCACCAGC ATGTTGGATT TTTCTAATCC CTCAGATATT
    CCTGATCAAG TTATAAGAGT TTTCAAAGCA GATCAGCAAA GTTGCTACAT TATCATCAGT
    AAAGACACTA CAGCTAAGGA AGTTGTCTGT CAAGCTATTC ATGAATTTGG TTTGACTGGT
    GCATCTGACA CATACTCTCT CTGTGAAGTT TCTGTTACTC CTGAGGGTGT TATAAAACAG
    AGAAGACTTC CAGACCAATT CTCCAAATTA GCTGATAGAA TTCAGCTCAA TGGAAGGTAT
    TATTTGAAAA ATAACATGGA AACAGAAACC CTATGTTCAG ATGAAGATGC TCAAGAACTA
    GTTAAGGAAA GCCAGCTGTC CATGCTACAA CTAAGTACTA TTGAGGTGGC CACTCAGCTG
    TCAATGAGGG ACTTTGATTT ATTTCGTAAT ATTGAGCCTA CTGAATATAT TGATGACCTT
    TTTAAGTTAG AGTCCAAAAC AGGAAATACT CACTTGAAAG AATTTGAAGA CATTGTAAAC
    CAAGAGACAT TCTGGGTTGC ATCAGAGATT TTAACTGAAT CAAATCAGCT CAAACGAATG
    AAGATTATTA AACATTTTAT TAAAATTGCA CTTCATTGTC GAGAATGTAA GAACTTCAAT
    TCCATGTTTG CAATAATAAG TGGTTTGAAC TTGGCATCTG TAGCACGACT CAGAGGTACT
    TGGGAAAAGT TACCAAGCAA GTATGATAAA CACTTCCAAG ATCTGCAAGA CCTTTTTGAT
    CCATCCAGAA ACATGGCAAA ATACAGAAAT ATTCTTAGTA GTCAAAGCAT GCAGCCTCCA
    ATTATTCCAC TCTTTCCTGT TGTCAAGAAA GATATGACAT TTCTACATGA AGGAAATGAT
    TCCAAAGTTG ATGGTTTGGT AAATTTCGAG AAACTCAGAA TGATTGCCAA GGAAATTCGT
    CAGGTTGTTC GAATGACTTC TGCTAACATG GATCCAGCTA TGATGTTCCG ACAGAGGAAG
    AAGAGGTGGC GGAGTCTGGG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850812.1
    CDS237..4979
    Translation

    Target ORF information:

    RefSeq Version XM_024995044.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT
    GTATTAGAGC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGATAATATT
    CTACAAAGAG AAATTCCTGC CAGACAGTCT CGAAGAAGAT TTCGGAAAAT TAATTGTAAA
    GGAGAACGCC AGACTATTAC TGATGATGTG GATATTAACA GCTATCTTTC TCTTCCAGCC
    GATCTTACCA AGATGCACAT TGCAGACAAC CCTCATCCAC AGGTGACTCA CGTGTCTTCT
    AGTCAGTCTG GCTGTAGCAT CGCCAGTGAC TCTGGAAGCA GCAGTTTATC TGATATCTAT
    CAGGCTACCG AAAGTGAGGT AGGAGATGTC GATTTAACAC GTCTTCCAGA AGGACCTGTT
    GATTCTGAAG ATGAAGAAGA GGAAGATGAA GACATTGATC GAACAGATCC TCTGCAAGGG
    CGGGATCTTG TCCGAGAATG TCTTGAAAAA GAACCTGCAG ATAAAACTGA TGATGACATT
    GAACAATTGC TGGAGTTTAT GCACCAGCTT CCCGCTTTTG CAAATATGAC CATGTCTGTA
    AGGAGAGAAC TCTGCTCAGT GATGATTTTT GAAGTGGTAG AGCAGGCTGG AGCTGTCATT
    CTTGAAGATG GGCAAGAGCT TGATTCATGG TATGTTATTT TAAATGGCAC TGTGGAAATC
    AGTCATCCAG ATGGAAAAGT TGACAATCTC TTTATGGGAA ATAGTTTTGG AATCACACCC
    ACTCTGGATA AACAGTACAT GCATGGAGTT GTTAGGACTA AAGTAGATGA CTGTCAGTTT
    GTCTGCATAG CCCAGCAGGA TTATTGGAGA ATTTTAAACC ATGTTGAAAA AAATACCCAT
    AAAGTTGAGG AAGAGGGAGA AATTGTTATG GTACATGAGC ATCGTGAACT GGATCGAAGT
    GGAACCAGGA AAGGACACAT TGTAATTAAG GGAACACCTG AGCGTCTCAT CATGCATTTA
    ATAGAAGAGC ATTCCATTGT GGACCCAACT TATATAGAAG ATTTCCTATT AACTTACAGA
    ACATTTCTTG CAAGTCCTTT GGATGTTGGG ACCAAACTGT TGGAATGGTT TAAAATTGAC
    AGCTTAAGGG ATAAGGTAAC ACGGATTGTA TTATTATGGG TAAATAATCA TTTCAATGAT
    TTTGAAGGTG ATCCTGCTAT GACTCGATTT CTAGAGGAAT TTGAAAAAAA TCTGGAAGAT
    ACAAAAATGA ATGGTCATCT CCGGTTATTG AATATTGCGT GTGCTGCAAA GGCTAAGTGG
    AGACAGGTTG TGCTGCAGAA AGCTTCCCGG GAGTCACCTC TGCATTTCAG CCTAAGTGGA
    GGAAGTGAGA AGGGATTTGG TATTTTTGTT GAAAGCGTAG AACCTAGTAG CAAAGCTGCT
    GATGCAGGAC TGAAACGTGG TGATCAAGTA ATGGAAGTAA ATGGACAAAA TTTTGAGAAT
    ATAACGTTTG TGAAGGCCCT TGAAATTTTG AGGAATAATA CTCATCTTGC ACTTACTGTA
    AAGACCAACA TTTTTGTGTT CAAAGAGTTA CTTAGTAGGA CTGAACAAGA GAAATCTGGT
    GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCACTCAAT CCAGGATGTA
    CCAGGAGATC TTGAACAGAC ATCACAGGAG AAAGGAAATA AGAAAATAAA AGCAAATACC
    GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG
    CCTCCAAAAC TTTTTAGTGA TGGAGGCCTG AGCCAGTCAC AGGATGACAG CATTGTGGGA
    ACGAGACACT GCAGGCACAG TCTGGCCATC ATGCCTATCC CTGGAACACT CTCGTCCAGC
    AGCCCCGACC TCCTCCAGCC TACCACCAGC ATGTTGGATT TTTCTAATCC CTCAGATATT
    CCTGATCAAG TTATAAGAGT TTTCAAAGCA GATCAGCAAA GTTGCTACAT TATCATCAGT
    AAAGACACTA CAGCTAAGGA AGTTGTCTGT CAAGCTATTC ATGAATTTGG TTTGACTGGT
    GCATCTGACA CATACTCTCT CTGTGAAGTT TCTGTTACTC CTGAGGGTGT TATAAAACAG
    AGAAGACTTC CAGACCAATT CTCCAAATTA GCTGATAGAA TTCAGCTCAA TGGAAGGTAT
    TATTTGAAAA ATAACATGGA AACAGAAACC CTATGTTCAG ATGAAGATGC TCAAGAACTA
    GTTAAGGAAA GCCAGCTGTC CATGCTACAA CTAAGTACTA TTGAGGTGGC CACTCAGCTG
    TCAATGAGGG ACTTTGATTT ATTTCGTAAT ATTGAGCCTA CTGAATATAT TGATGACCTT
    TTTAAGTTAG AGTCCAAAAC AGGAAATACT CACTTGAAAG AATTTGAAGA CATTGTAAAC
    CAAGAGACAT TCTGGGTTGC ATCAGAGATT TTAACTGAAT CAAATCAGCT CAAACGAATG
    AAGATTATTA AACATTTTAT TAAAATTGCA CTTCATTGTC GAGAATGTAA GAACTTCAAT
    TCCATGTTTG CAATAATAAG TGGTTTGAAC TTGGCATCTG TAGCACGACT CAGAGGTACT
    TGGGAAAAGT TACCAAGCAA GTATGATAAA CACTTCCAAG ATCTGCAAGA CCTTTTTGAT
    CCATCCAGAA ACATGGCAAA ATACAGAAAT ATTCTTAGTA GTCAAAGCAT GCAGCCTCCA
    ATTATTCCAC TCTTTCCTGT TGTCAAGAAA GATATGACAT TTCTACATGA AGGAAATGAT
    TCCAAAGTTG ATGGTTTGGT AAATTTCGAG AAACTCAGAA TGATTGCCAA GGAAATTCGT
    CAGGTTGTTC GAATGACTTC TGCTAACATG GATCCAGCTA TGATGTTCCG ACAGAGGAAG
    AAGAGGTGGC GGAGTCTGGG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236584
    Clone ID Related Accession (Same CDS sequence) XM_024995045.1
    Accession Version XM_024995045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCAGTG CCACCAGCAG AGAGGAGGAA ATGGGGAAAG CGAGATCTAG AGAGAGTTCA 
    GAAACTATTG CCAGATGTTG GTATATCCTG CTTTCTGGAT CTGTGCTTAT GAAAGACTCC
    ATGGTTTTGC CTCCTTGCAG TTTTGGTAAG CAGTTTGGAG GAAAAAGAGG ATGTGATTGT
    CTTGTATTAG AGCCTTCAGA AATGATTGTG GTAGAGAATT CCAAAGATAA TGAAGATAAT
    ATTCTACAAA GAGAAATTCC TGCCAGACAG TCTCGAAGAA GATTTCGGAA AATTAATTGT
    AAAGGAGAAC GCCAGACTAT TACTGATGAT GTGGATATTA ACAGCTATCT TTCTCTTCCA
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850813.1
    CDS16..4701
    Translation

    Target ORF information:

    RefSeq Version XM_024995045.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCTCAGTG CCACCAGCAG AGAGGAGGAA ATGGGGAAAG CGAGATCTAG AGAGAGTTCA 
    GAAACTATTG CCAGATGTTG GTATATCCTG CTTTCTGGAT CTGTGCTTAT GAAAGACTCC
    ATGGTTTTGC CTCCTTGCAG TTTTGGTAAG CAGTTTGGAG GAAAAAGAGG ATGTGATTGT
    CTTGTATTAG AGCCTTCAGA AATGATTGTG GTAGAGAATT CCAAAGATAA TGAAGATAAT
    ATTCTACAAA GAGAAATTCC TGCCAGACAG TCTCGAAGAA GATTTCGGAA AATTAATTGT
    AAAGGAGAAC GCCAGACTAT TACTGATGAT GTGGATATTA ACAGCTATCT TTCTCTTCCA
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236585
    Clone ID Related Accession (Same CDS sequence) XM_015472120.2
    Accession Version XM_015472120.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA
    AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG
    GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC
    CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG
    AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA
    ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA
    AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT
    TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA
    GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC
    CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC
    CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT
    ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA
    GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG
    TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC
    AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT
    TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC
    TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA
    CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT
    AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT
    GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT
    GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT
    TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG
    GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG
    ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC
    TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA
    GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327606.2
    CDS147..4688
    Translation

    Target ORF information:

    RefSeq Version XM_015472120.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472120.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA
    AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG
    GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC
    CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG
    AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA
    ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA
    AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT
    TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA
    GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC
    CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC
    CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT
    ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA
    GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG
    TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC
    AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT
    TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC
    TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA
    CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT
    AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT
    GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT
    GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT
    TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG
    GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG
    ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC
    TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA
    GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236586
    Clone ID Related Accession (Same CDS sequence) XM_005209077.4
    Accession Version XM_005209077.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209077.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209134.2
    CDS193..4728
    Translation

    Target ORF information:

    RefSeq Version XM_005209077.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209077.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236587
    Clone ID Related Accession (Same CDS sequence) XM_024995046.1
    Accession Version XM_024995046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4533bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCCTGT TTAAACTCCA CAGACTGTCA GTAATATGGA AAAATTGGAT GTTTAATCAG 
    GAATCTGAAC ATGTGTTTCA GGTAGAGAAT TCCAAAGATA ATGAAGATAA TATTCTACAA
    AGAGAAATTC CTGCCAGACA GTCTCGAAGA AGATTTCGGA AAATTAATTG TAAAGGAGAA
    CGCCAGACTA TTACTGATGA TGTGGATATT AACAGCTATC TTTCTCTTCC AGCCGATCTT
    ACCAAGATGC ACATTGCAGA CAACCCTCAT CCACAGGTGA CTCACGTGTC TTCTAGTCAG
    TCTGGCTGTA GCATCGCCAG TGACTCTGGA AGCAGCAGTT TATCTGATAT CTATCAGGCT
    ACCGAAAGTG AGGTAGGAGA TGTCGATTTA ACACGTCTTC CAGAAGGACC TGTTGATTCT
    GAAGATGAAG AAGAGGAAGA TGAAGACATT GATCGAACAG ATCCTCTGCA AGGGCGGGAT
    CTTGTCCGAG AATGTCTTGA AAAAGAACCT GCAGATAAAA CTGATGATGA CATTGAACAA
    TTGCTGGAGT TTATGCACCA GCTTCCCGCT TTTGCAAATA TGACCATGTC TGTAAGGAGA
    GAACTCTGCT CAGTGATGAT TTTTGAAGTG GTAGAGCAGG CTGGAGCTGT CATTCTTGAA
    GATGGGCAAG AGCTTGATTC ATGGTATGTT ATTTTAAATG GCACTGTGGA AATCAGTCAT
    CCAGATGGAA AAGTTGACAA TCTCTTTATG GGAAATAGTT TTGGAATCAC ACCCACTCTG
    GATAAACAGT ACATGCATGG AGTTGTTAGG ACTAAAGTAG ATGACTGTCA GTTTGTCTGC
    ATAGCCCAGC AGGATTATTG GAGAATTTTA AACCATGTTG AAAAAAATAC CCATAAAGTT
    GAGGAAGAGG GAGAAATTGT TATGGTACAT GAGCATCGTG AACTGGATCG AAGTGGAACC
    AGGAAAGGAC ACATTGTAAT TAAGGGAACA CCTGAGCGTC TCATCATGCA TTTAATAGAA
    GAGCATTCCA TTGTGGACCC AACTTATATA GAAGATTTCC TATTAACTTA CAGAACATTT
    CTTGCAAGTC CTTTGGATGT TGGGACCAAA CTGTTGGAAT GGTTTAAAAT TGACAGCTTA
    AGGGATAAGG TAACACGGAT TGTATTATTA TGGGTAAATA ATCATTTCAA TGATTTTGAA
    GGTGATCCTG CTATGACTCG ATTTCTAGAG GAATTTGAAA AAAATCTGGA AGATACAAAA
    ATGAATGGTC ATCTCCGGTT ATTGAATATT GCGTGTGCTG CAAAGGCTAA GTGGAGACAG
    GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA CCTCTGCATT TCAGCCTAAG TGGAGGAAGT
    GAGAAGGGAT TTGGTATTTT TGTTGAAAGC GTAGAACCTA GTAGCAAAGC TGCTGATGCA
    GGACTGAAAC GTGGTGATCA AGTAATGGAA GTAAATGGAC AAAATTTTGA GAATATAACG
    TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT AATACTCATC TTGCACTTAC TGTAAAGACC
    AACATTTTTG TGTTCAAAGA GTTACTTAGT AGGACTGAAC AAGAGAAATC TGGTGTTCCT
    CATATTCCCA AAATTGCTGA AAAAAAAAGT AATCGCCACT CAATCCAGGA TGTACCAGGA
    GATCTTGAAC AGACATCACA GGAGAAAGGA AATAAGAAAA TAAAAGCAAA TACCGTTTCA
    GGTGGAAGAA ACAAAATCAG GAAAATTTTG GATAAAACAC GATTTAGTAT CTTGCCTCCA
    AAACTTTTTA GTGATGGAGG CCTGAGCCAG TCACAGGATG ACAGCATTGT GGGAACGAGA
    CACTGCAGGC ACAGTCTGGC CATCATGCCT ATCCCTGGAA CACTCTCGTC CAGCAGCCCC
    GACCTCCTCC AGCCTACCAC CAGCATGTTG GATTTTTCTA ATCCCTCAGA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAG CAAAGTTGCT ACATTATCAT CAGTAAAGAC
    ACTACAGCTA AGGAAGTTGT CTGTCAAGCT ATTCATGAAT TTGGTTTGAC TGGTGCATCT
    GACACATACT CTCTCTGTGA AGTTTCTGTT ACTCCTGAGG GTGTTATAAA ACAGAGAAGA
    CTTCCAGACC AATTCTCCAA ATTAGCTGAT AGAATTCAGC TCAATGGAAG GTATTATTTG
    AAAAATAACA TGGAAACAGA AACCCTATGT TCAGATGAAG ATGCTCAAGA ACTAGTTAAG
    GAAAGCCAGC TGTCCATGCT ACAACTAAGT ACTATTGAGG TGGCCACTCA GCTGTCAATG
    AGGGACTTTG ATTTATTTCG TAATATTGAG CCTACTGAAT ATATTGATGA CCTTTTTAAG
    TTAGAGTCCA AAACAGGAAA TACTCACTTG AAAGAATTTG AAGACATTGT AAACCAAGAG
    ACATTCTGGG TTGCATCAGA GATTTTAACT GAATCAAATC AGCTCAAACG AATGAAGATT
    ATTAAACATT TTATTAAAAT TGCACTTCAT TGTCGAGAAT GTAAGAACTT CAATTCCATG
    TTTGCAATAA TAAGTGGTTT GAACTTGGCA TCTGTAGCAC GACTCAGAGG TACTTGGGAA
    AAGTTACCAA GCAAGTATGA TAAACACTTC CAAGATCTGC AAGACCTTTT TGATCCATCC
    AGAAACATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTTC CTGTTGTCAA GAAAGATATG ACATTTCTAC ATGAAGGAAA TGATTCCAAA
    GTTGATGGTT TGGTAAATTT CGAGAAACTC AGAATGATTG CCAAGGAAAT TCGTCAGGTT
    GTTCGAATGA CTTCTGCTAA CATGGATCCA GCTATGATGT TCCGACAGAG GTCACTGAGT
    CAAGGCAGCA CAAATTCAAA CATGCTGGAT GTTCAGGGAG GTGCTCACAA AAAAAGGGCA
    CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG CTATATGAAG ATGCCCAAAT GGCAAGAAAA
    GTGAAGCAGT ATCTTTCCAG TCTGGATGTC GAGACAGATG AAGAGAAGTT CCAGATGATG
    TCATTGCAAT GGGAGCCTGC ATATGGTACC TTGACCAAGA ATTTAAATGA GAAAAAATCA
    ATGAAGTCAT CAGAAATGTC TCCGGTGCCA CTGAGGTCAG CTGGCCAAAC AACTAAAGCT
    CATTTGCATC AGCCTCACAG AGGAAGCCAG GTGCTTCAGG TGCCAGCTGT AAATTTGCAC
    CCTATCAGGA AGAAGGGCCA AGGAAAAGAC CCTGCCACCC TGAGTACAGG TTTACCTCAG
    AAAATTTTAG GAACAGCTGA AGAAGTAAGT GGTGGTAAGA AACACACAGA AGACACCGTT
    TCTGTGGCAT CATCCTTACA TTCCAGCCCT CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA
    GGTTATACAC TTATACCATC AGCTAAATCT GACAACTTGT CTGACTCCAG CCACAGTGAG
    ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT TGTTCTGTTG ACTCCATGTC TACAGCTCTA
    CAGGATGAAC GGTGTTCCTC CCAGGCTGTG GCAGTCCCTG AATCCACTGG AGCATTGGAA
    AAGACAGAGC ACTCCTCAGG GATAGGAGAC CATAGTCAGC TTGGCCCTGG GTGGACACTC
    TCAAAGCCAT CTCTAATCAA CAAGAGTTTA GCTGTCTCAT CTTCTGTGAG CAATGAAGAG
    ATTTCTCATG AGCACATCAT TATAGAAACA GCTGATAGTG GTCGTGGAAG TTGGACTTCC
    TGCTCAAGCA GTTCCCATGA CAACTTCCAA AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT
    TTGAACTCTT ACCGACCTAC CCATTTGGAT GGTCCCATAG CTGAAGTTGA ACCCACTGAC
    TGTGAGCACT ACAGCTGTCC CAAAGGCTGC TCTCGAACTT GTGGTCAGTG TAAAGGAAGC
    CTAGAGACTA ATCAGATGAG ACAGAGTTGG GCTTCCTCTA GTTCTCTGTC TGACACATAC
    GAACCAAACT ATGGGACAGT TAAACGGAGA GTGCTGGAGA ACACCCCAGC TGAATCATCT
    GAAGGCTTGG ACCACAAAGA GGGCATCGAC CCAGTCTATA AAACTGTTAC TTCAAGTACA
    GAAAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AGGAGGATAG GTATAGGGAG
    CCACCTCCCA CTCCTCCAGG ATACATGGGG ATTTCTTTAG CGGACCTAAA GGAAGGACCC
    CACCTCCACC TAAAACCTCC AGAGTATAGT GTGGCGGTGC AGAGGTCAAA GATGATGCAT
    AACAGCCTCT CTAGACTGCC ACCAGCTTCT CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA
    TCGAAGATTG TAATTCAGCC TCAGAGGCAT AAACTAGATG TGACTGATGG AGATAGCGAA
    GCAGATGAAA ATGAACAGGT TTCAGCAGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850814.1
    CDS98..4630
    Translation

    Target ORF information:

    RefSeq Version XM_024995046.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCCTGT TTAAACTCCA CAGACTGTCA GTAATATGGA AAAATTGGAT GTTTAATCAG 
    GAATCTGAAC ATGTGTTTCA GGTAGAGAAT TCCAAAGATA ATGAAGATAA TATTCTACAA
    AGAGAAATTC CTGCCAGACA GTCTCGAAGA AGATTTCGGA AAATTAATTG TAAAGGAGAA
    CGCCAGACTA TTACTGATGA TGTGGATATT AACAGCTATC TTTCTCTTCC AGCCGATCTT
    ACCAAGATGC ACATTGCAGA CAACCCTCAT CCACAGGTGA CTCACGTGTC TTCTAGTCAG
    TCTGGCTGTA GCATCGCCAG TGACTCTGGA AGCAGCAGTT TATCTGATAT CTATCAGGCT
    ACCGAAAGTG AGGTAGGAGA TGTCGATTTA ACACGTCTTC CAGAAGGACC TGTTGATTCT
    GAAGATGAAG AAGAGGAAGA TGAAGACATT GATCGAACAG ATCCTCTGCA AGGGCGGGAT
    CTTGTCCGAG AATGTCTTGA AAAAGAACCT GCAGATAAAA CTGATGATGA CATTGAACAA
    TTGCTGGAGT TTATGCACCA GCTTCCCGCT TTTGCAAATA TGACCATGTC TGTAAGGAGA
    GAACTCTGCT CAGTGATGAT TTTTGAAGTG GTAGAGCAGG CTGGAGCTGT CATTCTTGAA
    GATGGGCAAG AGCTTGATTC ATGGTATGTT ATTTTAAATG GCACTGTGGA AATCAGTCAT
    CCAGATGGAA AAGTTGACAA TCTCTTTATG GGAAATAGTT TTGGAATCAC ACCCACTCTG
    GATAAACAGT ACATGCATGG AGTTGTTAGG ACTAAAGTAG ATGACTGTCA GTTTGTCTGC
    ATAGCCCAGC AGGATTATTG GAGAATTTTA AACCATGTTG AAAAAAATAC CCATAAAGTT
    GAGGAAGAGG GAGAAATTGT TATGGTACAT GAGCATCGTG AACTGGATCG AAGTGGAACC
    AGGAAAGGAC ACATTGTAAT TAAGGGAACA CCTGAGCGTC TCATCATGCA TTTAATAGAA
    GAGCATTCCA TTGTGGACCC AACTTATATA GAAGATTTCC TATTAACTTA CAGAACATTT
    CTTGCAAGTC CTTTGGATGT TGGGACCAAA CTGTTGGAAT GGTTTAAAAT TGACAGCTTA
    AGGGATAAGG TAACACGGAT TGTATTATTA TGGGTAAATA ATCATTTCAA TGATTTTGAA
    GGTGATCCTG CTATGACTCG ATTTCTAGAG GAATTTGAAA AAAATCTGGA AGATACAAAA
    ATGAATGGTC ATCTCCGGTT ATTGAATATT GCGTGTGCTG CAAAGGCTAA GTGGAGACAG
    GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA CCTCTGCATT TCAGCCTAAG TGGAGGAAGT
    GAGAAGGGAT TTGGTATTTT TGTTGAAAGC GTAGAACCTA GTAGCAAAGC TGCTGATGCA
    GGACTGAAAC GTGGTGATCA AGTAATGGAA GTAAATGGAC AAAATTTTGA GAATATAACG
    TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT AATACTCATC TTGCACTTAC TGTAAAGACC
    AACATTTTTG TGTTCAAAGA GTTACTTAGT AGGACTGAAC AAGAGAAATC TGGTGTTCCT
    CATATTCCCA AAATTGCTGA AAAAAAAAGT AATCGCCACT CAATCCAGGA TGTACCAGGA
    GATCTTGAAC AGACATCACA GGAGAAAGGA AATAAGAAAA TAAAAGCAAA TACCGTTTCA
    GGTGGAAGAA ACAAAATCAG GAAAATTTTG GATAAAACAC GATTTAGTAT CTTGCCTCCA
    AAACTTTTTA GTGATGGAGG CCTGAGCCAG TCACAGGATG ACAGCATTGT GGGAACGAGA
    CACTGCAGGC ACAGTCTGGC CATCATGCCT ATCCCTGGAA CACTCTCGTC CAGCAGCCCC
    GACCTCCTCC AGCCTACCAC CAGCATGTTG GATTTTTCTA ATCCCTCAGA TATTCCTGAT
    CAAGTTATAA GAGTTTTCAA AGCAGATCAG CAAAGTTGCT ACATTATCAT CAGTAAAGAC
    ACTACAGCTA AGGAAGTTGT CTGTCAAGCT ATTCATGAAT TTGGTTTGAC TGGTGCATCT
    GACACATACT CTCTCTGTGA AGTTTCTGTT ACTCCTGAGG GTGTTATAAA ACAGAGAAGA
    CTTCCAGACC AATTCTCCAA ATTAGCTGAT AGAATTCAGC TCAATGGAAG GTATTATTTG
    AAAAATAACA TGGAAACAGA AACCCTATGT TCAGATGAAG ATGCTCAAGA ACTAGTTAAG
    GAAAGCCAGC TGTCCATGCT ACAACTAAGT ACTATTGAGG TGGCCACTCA GCTGTCAATG
    AGGGACTTTG ATTTATTTCG TAATATTGAG CCTACTGAAT ATATTGATGA CCTTTTTAAG
    TTAGAGTCCA AAACAGGAAA TACTCACTTG AAAGAATTTG AAGACATTGT AAACCAAGAG
    ACATTCTGGG TTGCATCAGA GATTTTAACT GAATCAAATC AGCTCAAACG AATGAAGATT
    ATTAAACATT TTATTAAAAT TGCACTTCAT TGTCGAGAAT GTAAGAACTT CAATTCCATG
    TTTGCAATAA TAAGTGGTTT GAACTTGGCA TCTGTAGCAC GACTCAGAGG TACTTGGGAA
    AAGTTACCAA GCAAGTATGA TAAACACTTC CAAGATCTGC AAGACCTTTT TGATCCATCC
    AGAAACATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT
    CCACTCTTTC CTGTTGTCAA GAAAGATATG ACATTTCTAC ATGAAGGAAA TGATTCCAAA
    GTTGATGGTT TGGTAAATTT CGAGAAACTC AGAATGATTG CCAAGGAAAT TCGTCAGGTT
    GTTCGAATGA CTTCTGCTAA CATGGATCCA GCTATGATGT TCCGACAGAG GTCACTGAGT
    CAAGGCAGCA CAAATTCAAA CATGCTGGAT GTTCAGGGAG GTGCTCACAA AAAAAGGGCA
    CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG CTATATGAAG ATGCCCAAAT GGCAAGAAAA
    GTGAAGCAGT ATCTTTCCAG TCTGGATGTC GAGACAGATG AAGAGAAGTT CCAGATGATG
    TCATTGCAAT GGGAGCCTGC ATATGGTACC TTGACCAAGA ATTTAAATGA GAAAAAATCA
    ATGAAGTCAT CAGAAATGTC TCCGGTGCCA CTGAGGTCAG CTGGCCAAAC AACTAAAGCT
    CATTTGCATC AGCCTCACAG AGGAAGCCAG GTGCTTCAGG TGCCAGCTGT AAATTTGCAC
    CCTATCAGGA AGAAGGGCCA AGGAAAAGAC CCTGCCACCC TGAGTACAGG TTTACCTCAG
    AAAATTTTAG GAACAGCTGA AGAAGTAAGT GGTGGTAAGA AACACACAGA AGACACCGTT
    TCTGTGGCAT CATCCTTACA TTCCAGCCCT CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA
    GGTTATACAC TTATACCATC AGCTAAATCT GACAACTTGT CTGACTCCAG CCACAGTGAG
    ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT TGTTCTGTTG ACTCCATGTC TACAGCTCTA
    CAGGATGAAC GGTGTTCCTC CCAGGCTGTG GCAGTCCCTG AATCCACTGG AGCATTGGAA
    AAGACAGAGC ACTCCTCAGG GATAGGAGAC CATAGTCAGC TTGGCCCTGG GTGGACACTC
    TCAAAGCCAT CTCTAATCAA CAAGAGTTTA GCTGTCTCAT CTTCTGTGAG CAATGAAGAG
    ATTTCTCATG AGCACATCAT TATAGAAACA GCTGATAGTG GTCGTGGAAG TTGGACTTCC
    TGCTCAAGCA GTTCCCATGA CAACTTCCAA AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT
    TTGAACTCTT ACCGACCTAC CCATTTGGAT GGTCCCATAG CTGAAGTTGA ACCCACTGAC
    TGTGAGCACT ACAGCTGTCC CAAAGGCTGC TCTCGAACTT GTGGTCAGTG TAAAGGAAGC
    CTAGAGACTA ATCAGATGAG ACAGAGTTGG GCTTCCTCTA GTTCTCTGTC TGACACATAC
    GAACCAAACT ATGGGACAGT TAAACGGAGA GTGCTGGAGA ACACCCCAGC TGAATCATCT
    GAAGGCTTGG ACCACAAAGA GGGCATCGAC CCAGTCTATA AAACTGTTAC TTCAAGTACA
    GAAAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AGGAGGATAG GTATAGGGAG
    CCACCTCCCA CTCCTCCAGG ATACATGGGG ATTTCTTTAG CGGACCTAAA GGAAGGACCC
    CACCTCCACC TAAAACCTCC AGAGTATAGT GTGGCGGTGC AGAGGTCAAA GATGATGCAT
    AACAGCCTCT CTAGACTGCC ACCAGCTTCT CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA
    TCGAAGATTG TAATTCAGCC TCAGAGGCAT AAACTAGATG TGACTGATGG AGATAGCGAA
    GCAGATGAAA ATGAACAGGT TTCAGCAGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236588
    Clone ID Related Accession (Same CDS sequence) XM_024995047.1
    Accession Version XM_024995047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGTCAC TGAGTCAAGG CAGCACAAAT
    TCAAACATGC TGGATGTTCA GGGAGGTGCT CACAAAAAAA GGGCACGCCG TAGCTCTCTG
    CTAAATGCCA AGAAGCTATA TGAAGATGCC CAAATGGCAA GAAAAGTGAA GCAGTATCTT
    TCCAGTCTGG ATGTCGAGAC AGATGAAGAG AAGTTCCAGA TGATGTCATT GCAATGGGAG
    CCTGCATATG GTACCTTGAC CAAGAATTTA AATGAGAAAA AATCAATGAA GTCATCAGAA
    ATGTCTCCGG TGCCACTGAG GTCAGCTGGC CAAACAACTA AAGCTCATTT GCATCAGCCT
    CACAGAGGAA GCCAGGTGCT TCAGGTGCCA GCTGTAAATT TGCACCCTAT CAGGAAGAAG
    GGCCAAGGAA AAGACCCTGC CACCCTGAGT ACAGGTTTAC CTCAGAAAAT TTTAGGAACA
    GCTGAAGAAG TAAGTGGTGG TAAGAAACAC ACAGAAGACA CCGTTTCTGT GGCATCATCC
    TTACATTCCA GCCCTCCTGT GTCTCCTCAA GGTTCCCCCC GCAAAGGTTA TACACTTATA
    CCATCAGCTA AATCTGACAA CTTGTCTGAC TCCAGCCACA GTGAGATTTC TTCACGGTCC
    AGCATTGTGA GCAATTGTTC TGTTGACTCC ATGTCTACAG CTCTACAGGA TGAACGGTGT
    TCCTCCCAGG CTGTGGCAGT CCCTGAATCC ACTGGAGCAT TGGAAAAGAC AGAGCACTCC
    TCAGGGATAG GAGACCATAG TCAGCTTGGC CCTGGGTGGA CACTCTCAAA GCCATCTCTA
    ATCAACAAGA GTTTAGCTGT CTCATCTTCT GTGAGCAATG AAGAGATTTC TCATGAGCAC
    ATCATTATAG AAACAGCTGA TAGTGGTCGT GGAAGTTGGA CTTCCTGCTC AAGCAGTTCC
    CATGACAACT TCCAAAGTCT TCCAAACCCC AAAGGCTGGG AGTTTTTGAA CTCTTACCGA
    CCTACCCATT TGGATGGTCC CATAGCTGAA GTTGAACCCA CTGACTGTGA GCACTACAGC
    TGTCCCAAAG GCTGCTCTCG AACTTGTGGT CAGTGTAAAG GAAGCCTAGA GACTAATCAG
    ATGAGACAGA GTTGGGCTTC CTCTAGTTCT CTGTCTGACA CATACGAACC AAACTATGGG
    ACAGTTAAAC GGAGAGTGCT GGAGAACACC CCAGCTGAAT CATCTGAAGG CTTGGACCAC
    AAAGAGGGCA TCGACCCAGT CTATAAAACT GTTACTTCAA GTACAGAAAA GGGCTTGATT
    GTGTACTGTG TCACCTCACC CAAGAAGGAG GATAGGTATA GGGAGCCACC TCCCACTCCT
    CCAGGATACA TGGGGATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCT CCACCTAAAA
    CCTCCAGAGT ATAGTGTGGC GGTGCAGAGG TCAAAGATGA TGCATAACAG CCTCTCTAGA
    CTGCCACCAG CTTCTCTCAG TAGCAGCCCT GTGGCCTGTG TTCCATCGAA GATTGTAATT
    CAGCCTCAGA GGCATAAACT AGATGTGACT GATGGAGATA GCGAAGCAGA TGAAAATGAA
    CAGGTTTCAG CAGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850815.1
    CDS451..4968
    Translation

    Target ORF information:

    RefSeq Version XM_024995047.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGTCAC TGAGTCAAGG CAGCACAAAT
    TCAAACATGC TGGATGTTCA GGGAGGTGCT CACAAAAAAA GGGCACGCCG TAGCTCTCTG
    CTAAATGCCA AGAAGCTATA TGAAGATGCC CAAATGGCAA GAAAAGTGAA GCAGTATCTT
    TCCAGTCTGG ATGTCGAGAC AGATGAAGAG AAGTTCCAGA TGATGTCATT GCAATGGGAG
    CCTGCATATG GTACCTTGAC CAAGAATTTA AATGAGAAAA AATCAATGAA GTCATCAGAA
    ATGTCTCCGG TGCCACTGAG GTCAGCTGGC CAAACAACTA AAGCTCATTT GCATCAGCCT
    CACAGAGGAA GCCAGGTGCT TCAGGTGCCA GCTGTAAATT TGCACCCTAT CAGGAAGAAG
    GGCCAAGGAA AAGACCCTGC CACCCTGAGT ACAGGTTTAC CTCAGAAAAT TTTAGGAACA
    GCTGAAGAAG TAAGTGGTGG TAAGAAACAC ACAGAAGACA CCGTTTCTGT GGCATCATCC
    TTACATTCCA GCCCTCCTGT GTCTCCTCAA GGTTCCCCCC GCAAAGGTTA TACACTTATA
    CCATCAGCTA AATCTGACAA CTTGTCTGAC TCCAGCCACA GTGAGATTTC TTCACGGTCC
    AGCATTGTGA GCAATTGTTC TGTTGACTCC ATGTCTACAG CTCTACAGGA TGAACGGTGT
    TCCTCCCAGG CTGTGGCAGT CCCTGAATCC ACTGGAGCAT TGGAAAAGAC AGAGCACTCC
    TCAGGGATAG GAGACCATAG TCAGCTTGGC CCTGGGTGGA CACTCTCAAA GCCATCTCTA
    ATCAACAAGA GTTTAGCTGT CTCATCTTCT GTGAGCAATG AAGAGATTTC TCATGAGCAC
    ATCATTATAG AAACAGCTGA TAGTGGTCGT GGAAGTTGGA CTTCCTGCTC AAGCAGTTCC
    CATGACAACT TCCAAAGTCT TCCAAACCCC AAAGGCTGGG AGTTTTTGAA CTCTTACCGA
    CCTACCCATT TGGATGGTCC CATAGCTGAA GTTGAACCCA CTGACTGTGA GCACTACAGC
    TGTCCCAAAG GCTGCTCTCG AACTTGTGGT CAGTGTAAAG GAAGCCTAGA GACTAATCAG
    ATGAGACAGA GTTGGGCTTC CTCTAGTTCT CTGTCTGACA CATACGAACC AAACTATGGG
    ACAGTTAAAC GGAGAGTGCT GGAGAACACC CCAGCTGAAT CATCTGAAGG CTTGGACCAC
    AAAGAGGGCA TCGACCCAGT CTATAAAACT GTTACTTCAA GTACAGAAAA GGGCTTGATT
    GTGTACTGTG TCACCTCACC CAAGAAGGAG GATAGGTATA GGGAGCCACC TCCCACTCCT
    CCAGGATACA TGGGGATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCT CCACCTAAAA
    CCTCCAGAGT ATAGTGTGGC GGTGCAGAGG TCAAAGATGA TGCATAACAG CCTCTCTAGA
    CTGCCACCAG CTTCTCTCAG TAGCAGCCCT GTGGCCTGTG TTCCATCGAA GATTGTAATT
    CAGCCTCAGA GGCATAAACT AGATGTGACT GATGGAGATA GCGAAGCAGA TGAAAATGAA
    CAGGTTTCAG CAGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90291
    Clone ID Related Accession (Same CDS sequence) XM_015472120.1 , XM_015464187.1
    Accession Version XM_015472120.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA
    AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG
    GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC
    CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG
    AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA
    ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA
    AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT
    TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA
    GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC
    CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC
    CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT
    ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA
    GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG
    TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC
    AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT
    TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC
    TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA
    CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT
    AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT
    GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT
    GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT
    TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG
    GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG
    ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC
    TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA
    GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327606.1
    CDS128..4669
    Translation

    Target ORF information:

    RefSeq Version XM_015472120.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472120.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA
    AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG
    GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC
    CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG
    AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA
    ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA
    AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT
    TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA
    GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC
    CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC
    CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT
    ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA
    GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG
    TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC
    AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT
    TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC
    TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA
    CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT
    AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT
    GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT
    GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT
    TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG
    GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG
    ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC
    TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA
    GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90291
    Clone ID Related Accession (Same CDS sequence) XM_015472120.1 , XM_015464187.1
    Accession Version XM_015464187.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA
    AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG
    GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC
    CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG
    AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA
    ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA
    AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT
    TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA
    GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC
    CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC
    CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT
    ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA
    GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG
    TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC
    AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT
    TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC
    TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA
    CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT
    AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT
    GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT
    GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT
    TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG
    GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG
    ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC
    TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA
    GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319673.1
    CDS128..4669
    Translation

    Target ORF information:

    RefSeq Version XM_015464187.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464187.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
    TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC
    AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT
    GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT
    GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC
    GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA
    GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG
    GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT
    CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG
    CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG
    ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT
    GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT
    GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG
    CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT
    TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA
    ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT
    GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG
    GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG
    GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA
    CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG
    ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC
    CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA
    GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT
    ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT
    GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT
    GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC
    AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT
    GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA
    TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA
    ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT
    GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT
    CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT
    ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT
    TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA
    GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC
    TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC
    TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA
    ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC
    ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA
    TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA
    GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA
    TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT
    AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT
    GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG
    TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA
    TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT
    GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA
    AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT
    GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG
    TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA
    AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG
    GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC
    CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG
    AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA
    ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA
    AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT
    TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA
    GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC
    CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC
    CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT
    ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA
    GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG
    TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC
    AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT
    TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC
    TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA
    CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT
    AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT
    GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT
    GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT
    TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG
    TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG
    GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG
    ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC
    TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA
    GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21248
    Clone ID Related Accession (Same CDS sequence) XM_015464185.1 , XM_003586275.4
    Accession Version XM_015464185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT
    CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC
    CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC
    CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG
    ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT
    GAAAATGAAC AGGTTTCAGC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319671.1
    CDS224..5050
    Translation

    Target ORF information:

    RefSeq Version XM_015464185.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT
    CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC
    CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC
    CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG
    ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT
    GAAAATGAAC AGGTTTCAGC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21248
    Clone ID Related Accession (Same CDS sequence) XM_015464185.1 , XM_003586275.4
    Accession Version XM_003586275.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586275.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT
    CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC
    CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC
    CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG
    ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT
    GAAAATGAAC AGGTTTCAGC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586323.1
    CDS224..5050
    Translation

    Target ORF information:

    RefSeq Version XM_003586275.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586275.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT
    CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC
    CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC
    CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG
    ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT
    GAAAATGAAC AGGTTTCAGC AGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21249
    Clone ID Related Accession (Same CDS sequence) XM_015464186.1 , XM_002688965.5
    Accession Version XM_015464186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319672.1
    CDS223..5025
    Translation

    Target ORF information:

    RefSeq Version XM_015464186.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21249
    Clone ID Related Accession (Same CDS sequence) XM_015464186.1 , XM_002688965.5
    Accession Version XM_002688965.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002688965.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002689011.2
    CDS223..5025
    Translation

    Target ORF information:

    RefSeq Version XM_002688965.5
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002688965.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT
    GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG
    CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC
    GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC
    TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA
    CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG
    GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC
    CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT
    GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT
    CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT
    GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT
    TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG
    GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC
    CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA
    GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA
    GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA
    AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT
    GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC
    TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG
    GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA
    GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC
    CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC
    TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG
    ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT
    GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT
    CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT
    AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21251
    Clone ID Related Accession (Same CDS sequence) XM_005209077.3 , XM_015464188.1
    Accession Version XM_005209077.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209134.1
    CDS224..4759
    Translation

    Target ORF information:

    RefSeq Version XM_005209077.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209077.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21251
    Clone ID Related Accession (Same CDS sequence) XM_005209077.3 , XM_015464188.1
    Accession Version XM_015464188.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4536bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319674.1
    CDS224..4759
    Translation

    Target ORF information:

    RefSeq Version XM_015464188.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464188.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
    ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT
    CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC
    AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA
    TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA
    GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT
    TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA
    AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT
    AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT
    CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA
    AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA
    ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT
    GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT
    GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT
    TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG
    GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT
    ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG
    GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG
    ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA
    AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT
    GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA
    CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA
    GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA
    CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA
    TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG
    GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT
    GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA
    CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC
    GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA
    GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT
    AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT
    AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT
    AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA
    AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG
    GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG
    TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT
    ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG
    GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG
    CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT
    ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT
    ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT
    AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT
    TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT
    ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG
    CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG
    AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT
    GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT
    TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA
    TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC
    CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT
    AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG
    ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC
    AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA
    GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC
    AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT
    AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG
    CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG
    CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG
    TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG
    CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC
    AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT
    TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG
    GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT
    ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT
    TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT
    GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA
    GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG
    CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT
    CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA
    AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC
    TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG
    CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG
    ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA
    AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC
    TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG
    GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21253
    Clone ID Related Accession (Same CDS sequence) XM_015464189.1 , XM_005209079.3
    Accession Version XM_015464189.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319675.1
    CDS861..5246
    Translation

    Target ORF information:

    RefSeq Version XM_015464189.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464189.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21253
    Clone ID Related Accession (Same CDS sequence) XM_015464189.1 , XM_005209079.3
    Accession Version XM_005209079.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rap guanine nucleotide exchange factor 6 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209136.1
    CDS861..5246
    Translation

    Target ORF information:

    RefSeq Version XM_005209079.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209079.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
    GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT
    TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC
    TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT
    GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA
    GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC
    ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT
    GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC
    ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA
    ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA
    CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG
    TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC
    CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA
    AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT
    TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC
    AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT
    GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT
    GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA
    GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG
    TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT
    GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT
    GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG
    AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT
    GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT
    GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT
    GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT
    ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC
    TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG
    GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC
    AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT
    ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC
    AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT
    GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA
    CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG
    TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA
    CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG
    CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC
    CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA
    AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA
    ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC
    AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT
    ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT
    GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT
    CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT
    GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT
    CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG
    AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG
    GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG
    AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT
    GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT
    ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG
    CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC
    CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA
    GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA
    AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC
    CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA
    TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC
    AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT
    GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA
    GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT
    TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA
    ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC
    CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG
    GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC
    TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT
    TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG
    AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC
    GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC
    ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG
    GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT
    AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT
    TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG
    CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.