RAPGEF6 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015464186.1	XP_015319672.1	rap guanine nucleotide exchange factor 6 isoform X2
XM_005209077.3	XP_005209134.1	rap guanine nucleotide exchange factor 6 isoform X4
XM_024995048.1	XP_024850816.1	rap guanine nucleotide exchange factor 6 isoform X10
XM_024995050.1	XP_024850818.1	rap guanine nucleotide exchange factor 6 isoform X12
XM_002688965.5	XP_002689011.2	rap guanine nucleotide exchange factor 6 isoform X2
XM_015464185.1	XP_015319671.1	rap guanine nucleotide exchange factor 6 isoform X1
XM_015464189.1	XP_015319675.1	rap guanine nucleotide exchange factor 6 isoform X5
XM_003586275.5	XP_003586323.2	rap guanine nucleotide exchange factor 6 isoform X1
XM_005209079.4	XP_005209136.2	rap guanine nucleotide exchange factor 6 isoform X9
XM_003586275.4	XP_003586323.1	rap guanine nucleotide exchange factor 6 isoform X1
XM_005209079.3	XP_005209136.1	rap guanine nucleotide exchange factor 6 isoform X5
XM_015464188.1	XP_015319674.1	rap guanine nucleotide exchange factor 6 isoform X4
XM_024995044.1	XP_024850812.1	rap guanine nucleotide exchange factor 6 isoform X3
XM_024995047.1	XP_024850815.1	rap guanine nucleotide exchange factor 6 isoform X8
XM_015472120.1	XP_015327606.1	rap guanine nucleotide exchange factor 6 isoform X3
XM_015472120.2	XP_015327606.2	rap guanine nucleotide exchange factor 6 isoform X5
XM_005209077.4	XP_005209134.2	rap guanine nucleotide exchange factor 6 isoform X6
XM_015464187.1	XP_015319673.1	rap guanine nucleotide exchange factor 6 isoform X3
XM_002688965.6	XP_002689011.3	rap guanine nucleotide exchange factor 6 isoform X2
XM_024995045.1	XP_024850813.1	rap guanine nucleotide exchange factor 6 isoform X4
XM_024995046.1	XP_024850814.1	rap guanine nucleotide exchange factor 6 isoform X7
XM_024995049.1	XP_024850817.1	rap guanine nucleotide exchange factor 6 isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236589	XM_005209079.4 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236590	XM_024995048.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236591	XM_024995049.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236592	XM_024995050.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa236581	XM_003586275.5 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236582	XM_002688965.6 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236583	XM_024995044.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236584	XM_024995045.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236585	XM_015472120.2 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236586	XM_005209077.4 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236587	XM_024995046.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa236588	XM_024995047.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa90291	XM_015472120.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa90291	XM_015464187.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OBa21248	XM_003586275.4 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa21248	XM_015464185.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa21249	XM_015464186.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa21249	XM_002688965.5 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa21251	XM_005209077.3 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa21251	XM_015464188.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa21253	XM_015464189.1 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa21253	XM_005209079.3 Latest version!	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

RAPGEF6 ( XM_005209079.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209136.2 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa236589
Clone ID Related Accession (Same CDS sequence)	XM_005209079.4
Accession Version	XM_005209079.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209079.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209136.2
CDS	98..4483
Translation	MAFLVRCYANCLQPWASKLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_005209079.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209079.4

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_024995048.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850816.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa236590
Clone ID Related Accession (Same CDS sequence)	XM_024995048.1
Accession Version	XM_024995048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGTTGGC 
AGTATCCTAT CTGATCACAG CATCCTGCTG CGCCTCTCAG GGTCTTCCTC ATCCCCTCCC 
CGTGAGTCCA GCACCAACAT CAGTGGCCAG AGCTGCCCCG GAATAGGTGG GGTTTACTTG 
CAAAAGAAAA TCCTTCAGAT TACCAGGAGC ACAGCCAAAA GAACAGACAG GACCGAGAAG 
GCCACTGAGG AGAATAGAGA CAGGACAAGC TGTGACAACA CAGCCAGAAA AAGAATGACA 
TCCCCGTTTA GAAGATTTAG GGAAAGAACT CTTTCAAGGG AGAGACTAGC CAACAGCAAA 
AAAGAGGACG CAGATCACAA TCAAGCTACA GAAAGCTGTG AGAAAGTGAA GGATGTTGGA 
AGCAACATCA AAGATGAGAA AGGGAGTGAC ATCTTCAATT CAAATAGCCG AGGCAACAGT 
AACACTTTAC ACTGCTTCTA TACGCGATTC ACGTCTAAAA GGAGAAAGAC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850816.1
CDS	193..4449
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKVGSILSDHSILLRLSGSSSSPPRESSTNISGQSCPGIGGVYLQKKILQITRSTAKRTDRTEKATEENRDRTSCDNTARKRMTSPFRRFRERTLSRERLANSKKEDADHNQATESCEKVKDVGSNIKDEKGSDIFNSNSRGNSNTLHCFYTRFTSKRRKTL

Target ORF information:

RefSeq Version	XM_024995048.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995048.1

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGTTGGC 
AGTATCCTAT CTGATCACAG CATCCTGCTG CGCCTCTCAG GGTCTTCCTC ATCCCCTCCC 
CGTGAGTCCA GCACCAACAT CAGTGGCCAG AGCTGCCCCG GAATAGGTGG GGTTTACTTG 
CAAAAGAAAA TCCTTCAGAT TACCAGGAGC ACAGCCAAAA GAACAGACAG GACCGAGAAG 
GCCACTGAGG AGAATAGAGA CAGGACAAGC TGTGACAACA CAGCCAGAAA AAGAATGACA 
TCCCCGTTTA GAAGATTTAG GGAAAGAACT CTTTCAAGGG AGAGACTAGC CAACAGCAAA 
AAAGAGGACG CAGATCACAA TCAAGCTACA GAAAGCTGTG AGAAAGTGAA GGATGTTGGA 
AGCAACATCA AAGATGAGAA AGGGAGTGAC ATCTTCAATT CAAATAGCCG AGGCAACAGT 
AACACTTTAC ACTGCTTCTA TACGCGATTC ACGTCTAAAA GGAGAAAGAC ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_024995049.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850817.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa236591
Clone ID Related Accession (Same CDS sequence)	XM_024995049.1
Accession Version	XM_024995049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GTTGGCAGTA TCCTATCTGA TCACAGCATC 
CTGCTGCGCC TCTCAGGGTC TTCCTCATCC CCTCCCCGTG AGTCCAGCAC CAACATCAGT 
GGCCAGAGCT GCCCCGGAAT AGGTGGGGTT TACTTGCAAA AGAAAATCCT TCAGATTACC 
AGGAGCACAG CCAAAAGAAC AGACAGGACC GAGAAGGCCA CTGAGGAGAA TAGAGACAGG 
ACAAGCTGTG ACAACACAGC CAGAAAAAGA ATGACATCCC CGTTTAGAAG ATTTAGGGAA 
AGAACTCTTT CAAGGGAGAG ACTAGCCAAC AGCAAAAAAG AGGACGCAGA TCACAATCAA 
GCTACAGAAA GCTGTGAGAA AGTGAAGGAT GTTGGAAGCA ACATCAAAGA TGAGAAAGGG 
AGTGACATCT TCAATTCAAA TAGCCGAGGC AACAGTAACA CTTTACACTG CTTCTATACG 
CGATTCACGT CTAAAAGGAG AAAGACACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850817.1
CDS	193..4425
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKVGSILSDHSILLRLSGSSSSPPRESSTNISGQSCPGIGGVYLQKKILQITRSTAKRTDRTEKATEENRDRTSCDNTARKRMTSPFRRFRERTLSRERLANSKKEDADHNQATESCEKVKDVGSNIKDEKGSDIFNSNSRGNSNTLHCFYTRFTSKRRKTL

Target ORF information:

RefSeq Version	XM_024995049.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995049.1

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GTTGGCAGTA TCCTATCTGA TCACAGCATC 
CTGCTGCGCC TCTCAGGGTC TTCCTCATCC CCTCCCCGTG AGTCCAGCAC CAACATCAGT 
GGCCAGAGCT GCCCCGGAAT AGGTGGGGTT TACTTGCAAA AGAAAATCCT TCAGATTACC 
AGGAGCACAG CCAAAAGAAC AGACAGGACC GAGAAGGCCA CTGAGGAGAA TAGAGACAGG 
ACAAGCTGTG ACAACACAGC CAGAAAAAGA ATGACATCCC CGTTTAGAAG ATTTAGGGAA 
AGAACTCTTT CAAGGGAGAG ACTAGCCAAC AGCAAAAAAG AGGACGCAGA TCACAATCAA 
GCTACAGAAA GCTGTGAGAA AGTGAAGGAT GTTGGAAGCA ACATCAAAGA TGAGAAAGGG 
AGTGACATCT TCAATTCAAA TAGCCGAGGC AACAGTAACA CTTTACACTG CTTCTATACG 
CGATTCACGT CTAAAAGGAG AAAGACACTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_024995050.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850818.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X12, mRNA.

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CloneID	OBa236592
Clone ID Related Accession (Same CDS sequence)	XM_024995050.1
Accession Version	XM_024995050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGTGG TAGAGAATTC CAAAGATAAT GAAGATAATA TTCTACAAAG AGAAATTCCT 
GCCAGACAGT CTCGAAGAAG ATTTCGGAAA ATTAATTGTA AAGGAGAACG CCAGACTATT 
ACTGATGATG TGGATATTAA CAGCTATCTT TCTCTTCCAG CCGATCTTAC CAAGATGCAC 
ATTGCAGACA ACCCTCATCC ACAGGTGACT CACGTGTCTT CTAGTCAGTC TGGCTGTAGC 
ATCGCCAGTG ACTCTGGAAG CAGCAGTTTA TCTGATATCT ATCAGGCTAC CGAAAGTGAG 
GTAGGAGATG TCGATTTAAC ACGTCTTCCA GAAGGACCTG TTGATTCTGA AGATGAAGAA 
GAGGAAGATG AAGACATTGA TCGAACAGAT CCTCTGCAAG GGCGGGATCT TGTCCGAGAA 
TGTCTTGAAA AAGAACCTGC AGATAAAACT GATGATGACA TTGAACAATT GCTGGAGTTT 
ATGCACCAGC TTCCCGCTTT TGCAAATATG ACCATGTCTG TAAGGAGAGA ACTCTGCTCA 
GTGATGATTT TTGAAGTGGT AGAGCAGGCT GGAGCTGTCA TTCTTGAAGA TGGGCAAGAG 
CTTGATTCAT GGTATGTTAT TTTAAATGGC ACTGTGGAAA TCAGTCATCC AGATGGAAAA 
GTTGACAATC TCTTTATGGG AAATAGTTTT GGAATCACAC CCACTCTGGA TAAACAGTAC 
ATGCATGGAG TTGTTAGGAC TAAAGTAGAT GACTGTCAGT TTGTCTGCAT AGCCCAGCAG 
GATTATTGGA GAATTTTAAA CCATGTTGAA AAAAATACCC ATAAAGTTGA GGAAGAGGGA 
GAAATTGTTA TGGTACATGA GCATCGTGAA CTGGATCGAA GTGGAACCAG GAAAGGACAC 
ATTGTAATTA AGGGAACACC TGAGCGTCTC ATCATGCATT TAATAGAAGA GCATTCCATT 
GTGGACCCAA CTTATATAGA AGATTTCCTA TTAACTTACA GAACATTTCT TGCAAGTCCT 
TTGGATGTTG GGACCAAACT GTTGGAATGG TTTAAAATTG ACAGCTTAAG GGATAAGGTA 
ACACGGATTG TATTATTATG GGTAAATAAT CATTTCAATG ATTTTGAAGG TGATCCTGCT 
ATGACTCGAT TTCTAGAGGA ATTTGAAAAA AATCTGGAAG ATACAAAAAT GAATGGTCAT 
CTCCGGTTAT TGAATATTGC GTGTGCTGCA AAGGCTAAGT GGAGACAGGT TGTGCTGCAG 
AAAGCTTCCC GGGAGTCACC TCTGCATTTC AGCCTAAGTG GAGGAAGTGA GAAGGGATTT 
GGTATTTTTG TTGAAAGCGT AGAACCTAGT AGCAAAGCTG CTGATGCAGG ACTGAAACGT 
GGTGATCAAG TAATGGAAGT AAATGGACAA AATTTTGAGA ATATAACGTT TGTGAAGGCC 
CTTGAAATTT TGAGGAATAA TACTCATCTT GCACTTACTG TAAAGACCAA CATTTTTGTG 
TTCAAAGAGT TACTTAGTAG GACTGAACAA GAGAAATCTG GTGTTCCTCA TATTCCCAAA 
ATTGCTGAAA AAAAAAGTAA TCGCCACTCA ATCCAGGATG TACCAGGAGA TCTTGAACAG 
ACATCACAGG AGAAAGGAAA TAAGAAAATA AAAGCAAATA CCGTTTCAGG TGGAAGAAAC 
AAAATCAGGA AAATTTTGGA TAAAACACGA TTTAGTATCT TGCCTCCAAA ACTTTTTAGT 
GATGGAGGCC TGAGCCAGTC ACAGGATGAC AGCATTGTGG GAACGAGACA CTGCAGGCAC 
AGTCTGGCCA TCATGCCTAT CCCTGGAACA CTCTCGTCCA GCAGCCCCGA CCTCCTCCAG 
CCTACCACCA GCATGTTGGA TTTTTCTAAT CCCTCAGATA TTCCTGATCA AGTTATAAGA 
GTTTTCAAAG CAGATCAGCA AAGTTGCTAC ATTATCATCA GTAAAGACAC TACAGCTAAG 
GAAGTTGTCT GTCAAGCTAT TCATGAATTT GGTTTGACTG GTGCATCTGA CACATACTCT 
CTCTGTGAAG TTTCTGTTAC TCCTGAGGGT GTTATAAAAC AGAGAAGACT TCCAGACCAA 
TTCTCCAAAT TAGCTGATAG AATTCAGCTC AATGGAAGGT ATTATTTGAA AAATAACATG 
GAAACAGAAA CCCTATGTTC AGATGAAGAT GCTCAAGAAC TAGTTAAGGA AAGCCAGCTG 
TCCATGCTAC AACTAAGTAC TATTGAGGTG GCCACTCAGC TGTCAATGAG GGACTTTGAT 
TTATTTCGTA ATATTGAGCC TACTGAATAT ATTGATGACC TTTTTAAGTT AGAGTCCAAA 
ACAGGAAATA CTCACTTGAA AGAATTTGAA GACATTGTAA ACCAAGAGAC ATTCTGGGTT 
GCATCAGAGA TTTTAACTGA ATCAAATCAG CTCAAACGAA TGAAGATTAT TAAACATTTT 
ATTAAAATTG CACTTCATTG TCGAGAATGT AAGAACTTCA ATTCCATGTT TGCAATAATA 
AGTGGTTTGA ACTTGGCATC TGTAGCACGA CTCAGAGGTA CTTGGGAAAA GTTACCAAGC 
AAGTATGATA AACACTTCCA AGATCTGCAA GACCTTTTTG ATCCATCCAG AAACATGGCA 
AAATACAGAA ATATTCTTAG TAGTCAAAGC ATGCAGCCTC CAATTATTCC ACTCTTTCCT 
GTTGTCAAGA AAGATATGAC ATTTCTACAT GAAGGAAATG ATTCCAAAGT TGATGGTTTG 
GTAAATTTCG AGAAACTCAG AATGATTGCC AAGGAAATTC GTCAGGTTGT TCGAATGACT 
TCTGCTAACA TGGATCCAGC TATGATGTTC CGACAGAGGA AGAAGAGGTG GCGGAGTCTG 
GGGTCACTGA GTCAAGGCAG CACAAATTCA AACATGCTGG ATGTTCAGGG AGGTGCTCAC 
AAAAAAAGGG CACGCCGTAG CTCTCTGCTA AATGCCAAGA AGCTATATGA AGATGCCCAA 
ATGGCAAGAA AAGTGAAGCA GTATCTTTCC AGTCTGGATG TCGAGACAGA TGAAGAGAAG 
TTCCAGATGA TGTCATTGCA ATGGGAGCCT GCATATGGTA CCTTGACCAA GAATTTAAAT 
GAGAAAAAAT CAATGAAGTC ATCAGAAATG TCTCCGGTGC CACTGAGGTC AGCTGGCCAA 
ACAACTAAAG CTCATTTGCA TCAGCCTCAC AGAGGAAGCC AGGTGCTTCA GGTGCCAGCT 
GTAAATTTGC ACCCTATCAG GAAGAAGGGC CAAGGAAAAG ACCCTGCCAC CCTGAGTACA 
GGTTTACCTC AGAAAATTTT AGGAACAGCT GAAGAAGTAA GTGGTGGTAA GAAACACACA 
GAAGACACCG TTTCTGTGGC ATCATCCTTA CATTCCAGCC CTCCTGTGTC TCCTCAAGGT 
TCCCCCCGCA AAGGTTATAC ACTTATACCA TCAGCTAAAT CTGACAACTT GTCTGACTCC 
AGCCACAGTG AGATTTCTTC ACGGTCCAGC ATTGTGAGCA ATTGTTCTGT TGACTCCATG 
TCTACAGCTC TACAGGATGA ACGGTGTTCC TCCCAGGCTG TGGCAGTCCC TGAATCCACT 
GGAGCATTGG AAAAGACAGA GCACTCCTCA GGGATAGGAG ACCATAGTCA GCTTGGCCCT 
GGGTGGACAC TCTCAAAGCC ATCTCTAATC AACAAGAGTT TAGCTGTCTC ATCTTCTGTG 
AGCAATGAAG AGATTTCTCA TGAGCACATC ATTATAGAAA CAGCTGATAG TGGTCGTGGA 
AGTTGGACTT CCTGCTCAAG CAGTTCCCAT GACAACTTCC AAAGTCTTCC AAACCCCAAA 
GGCTGGGAGT TTTTGAACTC TTACCGACCT ACCCATTTGG ATGGTCCCAT AGCTGAAGTT 
GAACCCACTG ACTGTGAGCA CTACAGCTGT CCCAAAGGCT GCTCTCGAAC TTGTGGTCAG 
TGTAAAGGAA GCCTAGAGAC TAATCAGATG AGACAGAGTT GGGCTTCCTC TAGTTCTCTG 
TCTGACACAT ACGAACCAAA CTATGGGACA GTTAAACGGA GAGTGCTGGA GAACACCCCA 
GCTGAATCAT CTGAAGGCTT GGACCACAAA GAGGGCATCG ACCCAGTCTA TAAAACTGTT 
ACTTCAAGTA CAGAAAAGGG CTTGATTGTG TACTGTGTCA CCTCACCCAA GAAGGAGGAT 
AGGTATAGGG AGCCACCTCC CACTCCTCCA GGATACATGG GGATTTCTTT AGCGGACCTA 
AAGGAAGGAC CCCACCTCCA CCTAAAACCT CCAGAGTATA GTGTGGCGGT GCAGAGGTCA 
AAGATGATGC ATAACAGCCT CTCTAGACTG CCACCAGCTT CTCTCAGTAG CAGCCCTGTG 
GCCTGTGTTC CATCGAAGAT TGTAATTCAG CCTCAGAGGC ATAAACTAGA TGTGACTGAT 
GGAGATAGCG AAGCAGATGA AAATGAACAG GTTTCAGCAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850818.1
CDS	124..4608
Translation	MIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_024995050.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995050.1

ORF Insert Sequence:

ATGATTGTGG TAGAGAATTC CAAAGATAAT GAAGATAATA TTCTACAAAG AGAAATTCCT 
GCCAGACAGT CTCGAAGAAG ATTTCGGAAA ATTAATTGTA AAGGAGAACG CCAGACTATT 
ACTGATGATG TGGATATTAA CAGCTATCTT TCTCTTCCAG CCGATCTTAC CAAGATGCAC 
ATTGCAGACA ACCCTCATCC ACAGGTGACT CACGTGTCTT CTAGTCAGTC TGGCTGTAGC 
ATCGCCAGTG ACTCTGGAAG CAGCAGTTTA TCTGATATCT ATCAGGCTAC CGAAAGTGAG 
GTAGGAGATG TCGATTTAAC ACGTCTTCCA GAAGGACCTG TTGATTCTGA AGATGAAGAA 
GAGGAAGATG AAGACATTGA TCGAACAGAT CCTCTGCAAG GGCGGGATCT TGTCCGAGAA 
TGTCTTGAAA AAGAACCTGC AGATAAAACT GATGATGACA TTGAACAATT GCTGGAGTTT 
ATGCACCAGC TTCCCGCTTT TGCAAATATG ACCATGTCTG TAAGGAGAGA ACTCTGCTCA 
GTGATGATTT TTGAAGTGGT AGAGCAGGCT GGAGCTGTCA TTCTTGAAGA TGGGCAAGAG 
CTTGATTCAT GGTATGTTAT TTTAAATGGC ACTGTGGAAA TCAGTCATCC AGATGGAAAA 
GTTGACAATC TCTTTATGGG AAATAGTTTT GGAATCACAC CCACTCTGGA TAAACAGTAC 
ATGCATGGAG TTGTTAGGAC TAAAGTAGAT GACTGTCAGT TTGTCTGCAT AGCCCAGCAG 
GATTATTGGA GAATTTTAAA CCATGTTGAA AAAAATACCC ATAAAGTTGA GGAAGAGGGA 
GAAATTGTTA TGGTACATGA GCATCGTGAA CTGGATCGAA GTGGAACCAG GAAAGGACAC 
ATTGTAATTA AGGGAACACC TGAGCGTCTC ATCATGCATT TAATAGAAGA GCATTCCATT 
GTGGACCCAA CTTATATAGA AGATTTCCTA TTAACTTACA GAACATTTCT TGCAAGTCCT 
TTGGATGTTG GGACCAAACT GTTGGAATGG TTTAAAATTG ACAGCTTAAG GGATAAGGTA 
ACACGGATTG TATTATTATG GGTAAATAAT CATTTCAATG ATTTTGAAGG TGATCCTGCT 
ATGACTCGAT TTCTAGAGGA ATTTGAAAAA AATCTGGAAG ATACAAAAAT GAATGGTCAT 
CTCCGGTTAT TGAATATTGC GTGTGCTGCA AAGGCTAAGT GGAGACAGGT TGTGCTGCAG 
AAAGCTTCCC GGGAGTCACC TCTGCATTTC AGCCTAAGTG GAGGAAGTGA GAAGGGATTT 
GGTATTTTTG TTGAAAGCGT AGAACCTAGT AGCAAAGCTG CTGATGCAGG ACTGAAACGT 
GGTGATCAAG TAATGGAAGT AAATGGACAA AATTTTGAGA ATATAACGTT TGTGAAGGCC 
CTTGAAATTT TGAGGAATAA TACTCATCTT GCACTTACTG TAAAGACCAA CATTTTTGTG 
TTCAAAGAGT TACTTAGTAG GACTGAACAA GAGAAATCTG GTGTTCCTCA TATTCCCAAA 
ATTGCTGAAA AAAAAAGTAA TCGCCACTCA ATCCAGGATG TACCAGGAGA TCTTGAACAG 
ACATCACAGG AGAAAGGAAA TAAGAAAATA AAAGCAAATA CCGTTTCAGG TGGAAGAAAC 
AAAATCAGGA AAATTTTGGA TAAAACACGA TTTAGTATCT TGCCTCCAAA ACTTTTTAGT 
GATGGAGGCC TGAGCCAGTC ACAGGATGAC AGCATTGTGG GAACGAGACA CTGCAGGCAC 
AGTCTGGCCA TCATGCCTAT CCCTGGAACA CTCTCGTCCA GCAGCCCCGA CCTCCTCCAG 
CCTACCACCA GCATGTTGGA TTTTTCTAAT CCCTCAGATA TTCCTGATCA AGTTATAAGA 
GTTTTCAAAG CAGATCAGCA AAGTTGCTAC ATTATCATCA GTAAAGACAC TACAGCTAAG 
GAAGTTGTCT GTCAAGCTAT TCATGAATTT GGTTTGACTG GTGCATCTGA CACATACTCT 
CTCTGTGAAG TTTCTGTTAC TCCTGAGGGT GTTATAAAAC AGAGAAGACT TCCAGACCAA 
TTCTCCAAAT TAGCTGATAG AATTCAGCTC AATGGAAGGT ATTATTTGAA AAATAACATG 
GAAACAGAAA CCCTATGTTC AGATGAAGAT GCTCAAGAAC TAGTTAAGGA AAGCCAGCTG 
TCCATGCTAC AACTAAGTAC TATTGAGGTG GCCACTCAGC TGTCAATGAG GGACTTTGAT 
TTATTTCGTA ATATTGAGCC TACTGAATAT ATTGATGACC TTTTTAAGTT AGAGTCCAAA 
ACAGGAAATA CTCACTTGAA AGAATTTGAA GACATTGTAA ACCAAGAGAC ATTCTGGGTT 
GCATCAGAGA TTTTAACTGA ATCAAATCAG CTCAAACGAA TGAAGATTAT TAAACATTTT 
ATTAAAATTG CACTTCATTG TCGAGAATGT AAGAACTTCA ATTCCATGTT TGCAATAATA 
AGTGGTTTGA ACTTGGCATC TGTAGCACGA CTCAGAGGTA CTTGGGAAAA GTTACCAAGC 
AAGTATGATA AACACTTCCA AGATCTGCAA GACCTTTTTG ATCCATCCAG AAACATGGCA 
AAATACAGAA ATATTCTTAG TAGTCAAAGC ATGCAGCCTC CAATTATTCC ACTCTTTCCT 
GTTGTCAAGA AAGATATGAC ATTTCTACAT GAAGGAAATG ATTCCAAAGT TGATGGTTTG 
GTAAATTTCG AGAAACTCAG AATGATTGCC AAGGAAATTC GTCAGGTTGT TCGAATGACT 
TCTGCTAACA TGGATCCAGC TATGATGTTC CGACAGAGGA AGAAGAGGTG GCGGAGTCTG 
GGGTCACTGA GTCAAGGCAG CACAAATTCA AACATGCTGG ATGTTCAGGG AGGTGCTCAC 
AAAAAAAGGG CACGCCGTAG CTCTCTGCTA AATGCCAAGA AGCTATATGA AGATGCCCAA 
ATGGCAAGAA AAGTGAAGCA GTATCTTTCC AGTCTGGATG TCGAGACAGA TGAAGAGAAG 
TTCCAGATGA TGTCATTGCA ATGGGAGCCT GCATATGGTA CCTTGACCAA GAATTTAAAT 
GAGAAAAAAT CAATGAAGTC ATCAGAAATG TCTCCGGTGC CACTGAGGTC AGCTGGCCAA 
ACAACTAAAG CTCATTTGCA TCAGCCTCAC AGAGGAAGCC AGGTGCTTCA GGTGCCAGCT 
GTAAATTTGC ACCCTATCAG GAAGAAGGGC CAAGGAAAAG ACCCTGCCAC CCTGAGTACA 
GGTTTACCTC AGAAAATTTT AGGAACAGCT GAAGAAGTAA GTGGTGGTAA GAAACACACA 
GAAGACACCG TTTCTGTGGC ATCATCCTTA CATTCCAGCC CTCCTGTGTC TCCTCAAGGT 
TCCCCCCGCA AAGGTTATAC ACTTATACCA TCAGCTAAAT CTGACAACTT GTCTGACTCC 
AGCCACAGTG AGATTTCTTC ACGGTCCAGC ATTGTGAGCA ATTGTTCTGT TGACTCCATG 
TCTACAGCTC TACAGGATGA ACGGTGTTCC TCCCAGGCTG TGGCAGTCCC TGAATCCACT 
GGAGCATTGG AAAAGACAGA GCACTCCTCA GGGATAGGAG ACCATAGTCA GCTTGGCCCT 
GGGTGGACAC TCTCAAAGCC ATCTCTAATC AACAAGAGTT TAGCTGTCTC ATCTTCTGTG 
AGCAATGAAG AGATTTCTCA TGAGCACATC ATTATAGAAA CAGCTGATAG TGGTCGTGGA 
AGTTGGACTT CCTGCTCAAG CAGTTCCCAT GACAACTTCC AAAGTCTTCC AAACCCCAAA 
GGCTGGGAGT TTTTGAACTC TTACCGACCT ACCCATTTGG ATGGTCCCAT AGCTGAAGTT 
GAACCCACTG ACTGTGAGCA CTACAGCTGT CCCAAAGGCT GCTCTCGAAC TTGTGGTCAG 
TGTAAAGGAA GCCTAGAGAC TAATCAGATG AGACAGAGTT GGGCTTCCTC TAGTTCTCTG 
TCTGACACAT ACGAACCAAA CTATGGGACA GTTAAACGGA GAGTGCTGGA GAACACCCCA 
GCTGAATCAT CTGAAGGCTT GGACCACAAA GAGGGCATCG ACCCAGTCTA TAAAACTGTT 
ACTTCAAGTA CAGAAAAGGG CTTGATTGTG TACTGTGTCA CCTCACCCAA GAAGGAGGAT 
AGGTATAGGG AGCCACCTCC CACTCCTCCA GGATACATGG GGATTTCTTT AGCGGACCTA 
AAGGAAGGAC CCCACCTCCA CCTAAAACCT CCAGAGTATA GTGTGGCGGT GCAGAGGTCA 
AAGATGATGC ATAACAGCCT CTCTAGACTG CCACCAGCTT CTCTCAGTAG CAGCCCTGTG 
GCCTGTGTTC CATCGAAGAT TGTAATTCAG CCTCAGAGGC ATAAACTAGA TGTGACTGAT 
GGAGATAGCG AAGCAGATGA AAATGAACAG GTTTCAGCAG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_003586275.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586323.2 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

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CloneID	OBa236581
Clone ID Related Accession (Same CDS sequence)	XM_003586275.5
Accession Version	XM_003586275.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586275.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT 
CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC 
CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC 
CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG 
ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT 
GAAAATGAAC AGGTTTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586323.2
CDS	237..5063
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_003586275.5
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586275.5

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT 
CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC 
CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC 
CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG 
ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT 
GAAAATGAAC AGGTTTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_002688965.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689011.3 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236582
Clone ID Related Accession (Same CDS sequence)	XM_002688965.6
Accession Version	XM_002688965.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002688965.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689011.3
CDS	211..5013
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_002688965.6
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002688965.6

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_024995044.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850812.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa236583
Clone ID Related Accession (Same CDS sequence)	XM_024995044.1
Accession Version	XM_024995044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT 
GTATTAGAGC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGATAATATT 
CTACAAAGAG AAATTCCTGC CAGACAGTCT CGAAGAAGAT TTCGGAAAAT TAATTGTAAA 
GGAGAACGCC AGACTATTAC TGATGATGTG GATATTAACA GCTATCTTTC TCTTCCAGCC 
GATCTTACCA AGATGCACAT TGCAGACAAC CCTCATCCAC AGGTGACTCA CGTGTCTTCT 
AGTCAGTCTG GCTGTAGCAT CGCCAGTGAC TCTGGAAGCA GCAGTTTATC TGATATCTAT 
CAGGCTACCG AAAGTGAGGT AGGAGATGTC GATTTAACAC GTCTTCCAGA AGGACCTGTT 
GATTCTGAAG ATGAAGAAGA GGAAGATGAA GACATTGATC GAACAGATCC TCTGCAAGGG 
CGGGATCTTG TCCGAGAATG TCTTGAAAAA GAACCTGCAG ATAAAACTGA TGATGACATT 
GAACAATTGC TGGAGTTTAT GCACCAGCTT CCCGCTTTTG CAAATATGAC CATGTCTGTA 
AGGAGAGAAC TCTGCTCAGT GATGATTTTT GAAGTGGTAG AGCAGGCTGG AGCTGTCATT 
CTTGAAGATG GGCAAGAGCT TGATTCATGG TATGTTATTT TAAATGGCAC TGTGGAAATC 
AGTCATCCAG ATGGAAAAGT TGACAATCTC TTTATGGGAA ATAGTTTTGG AATCACACCC 
ACTCTGGATA AACAGTACAT GCATGGAGTT GTTAGGACTA AAGTAGATGA CTGTCAGTTT 
GTCTGCATAG CCCAGCAGGA TTATTGGAGA ATTTTAAACC ATGTTGAAAA AAATACCCAT 
AAAGTTGAGG AAGAGGGAGA AATTGTTATG GTACATGAGC ATCGTGAACT GGATCGAAGT 
GGAACCAGGA AAGGACACAT TGTAATTAAG GGAACACCTG AGCGTCTCAT CATGCATTTA 
ATAGAAGAGC ATTCCATTGT GGACCCAACT TATATAGAAG ATTTCCTATT AACTTACAGA 
ACATTTCTTG CAAGTCCTTT GGATGTTGGG ACCAAACTGT TGGAATGGTT TAAAATTGAC 
AGCTTAAGGG ATAAGGTAAC ACGGATTGTA TTATTATGGG TAAATAATCA TTTCAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCGATTT CTAGAGGAAT TTGAAAAAAA TCTGGAAGAT 
ACAAAAATGA ATGGTCATCT CCGGTTATTG AATATTGCGT GTGCTGCAAA GGCTAAGTGG 
AGACAGGTTG TGCTGCAGAA AGCTTCCCGG GAGTCACCTC TGCATTTCAG CCTAAGTGGA 
GGAAGTGAGA AGGGATTTGG TATTTTTGTT GAAAGCGTAG AACCTAGTAG CAAAGCTGCT 
GATGCAGGAC TGAAACGTGG TGATCAAGTA ATGGAAGTAA ATGGACAAAA TTTTGAGAAT 
ATAACGTTTG TGAAGGCCCT TGAAATTTTG AGGAATAATA CTCATCTTGC ACTTACTGTA 
AAGACCAACA TTTTTGTGTT CAAAGAGTTA CTTAGTAGGA CTGAACAAGA GAAATCTGGT 
GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCACTCAAT CCAGGATGTA 
CCAGGAGATC TTGAACAGAC ATCACAGGAG AAAGGAAATA AGAAAATAAA AGCAAATACC 
GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG 
CCTCCAAAAC TTTTTAGTGA TGGAGGCCTG AGCCAGTCAC AGGATGACAG CATTGTGGGA 
ACGAGACACT GCAGGCACAG TCTGGCCATC ATGCCTATCC CTGGAACACT CTCGTCCAGC 
AGCCCCGACC TCCTCCAGCC TACCACCAGC ATGTTGGATT TTTCTAATCC CTCAGATATT 
CCTGATCAAG TTATAAGAGT TTTCAAAGCA GATCAGCAAA GTTGCTACAT TATCATCAGT 
AAAGACACTA CAGCTAAGGA AGTTGTCTGT CAAGCTATTC ATGAATTTGG TTTGACTGGT 
GCATCTGACA CATACTCTCT CTGTGAAGTT TCTGTTACTC CTGAGGGTGT TATAAAACAG 
AGAAGACTTC CAGACCAATT CTCCAAATTA GCTGATAGAA TTCAGCTCAA TGGAAGGTAT 
TATTTGAAAA ATAACATGGA AACAGAAACC CTATGTTCAG ATGAAGATGC TCAAGAACTA 
GTTAAGGAAA GCCAGCTGTC CATGCTACAA CTAAGTACTA TTGAGGTGGC CACTCAGCTG 
TCAATGAGGG ACTTTGATTT ATTTCGTAAT ATTGAGCCTA CTGAATATAT TGATGACCTT 
TTTAAGTTAG AGTCCAAAAC AGGAAATACT CACTTGAAAG AATTTGAAGA CATTGTAAAC 
CAAGAGACAT TCTGGGTTGC ATCAGAGATT TTAACTGAAT CAAATCAGCT CAAACGAATG 
AAGATTATTA AACATTTTAT TAAAATTGCA CTTCATTGTC GAGAATGTAA GAACTTCAAT 
TCCATGTTTG CAATAATAAG TGGTTTGAAC TTGGCATCTG TAGCACGACT CAGAGGTACT 
TGGGAAAAGT TACCAAGCAA GTATGATAAA CACTTCCAAG ATCTGCAAGA CCTTTTTGAT 
CCATCCAGAA ACATGGCAAA ATACAGAAAT ATTCTTAGTA GTCAAAGCAT GCAGCCTCCA 
ATTATTCCAC TCTTTCCTGT TGTCAAGAAA GATATGACAT TTCTACATGA AGGAAATGAT 
TCCAAAGTTG ATGGTTTGGT AAATTTCGAG AAACTCAGAA TGATTGCCAA GGAAATTCGT 
CAGGTTGTTC GAATGACTTC TGCTAACATG GATCCAGCTA TGATGTTCCG ACAGAGGAAG 
AAGAGGTGGC GGAGTCTGGG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850812.1
CDS	237..4979
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_024995044.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995044.1

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTT TGGTAAGCAG TTTGGAGGAA AAAGAGGATG TGATTGTCTT 
GTATTAGAGC CTTCAGAAAT GATTGTGGTA GAGAATTCCA AAGATAATGA AGATAATATT 
CTACAAAGAG AAATTCCTGC CAGACAGTCT CGAAGAAGAT TTCGGAAAAT TAATTGTAAA 
GGAGAACGCC AGACTATTAC TGATGATGTG GATATTAACA GCTATCTTTC TCTTCCAGCC 
GATCTTACCA AGATGCACAT TGCAGACAAC CCTCATCCAC AGGTGACTCA CGTGTCTTCT 
AGTCAGTCTG GCTGTAGCAT CGCCAGTGAC TCTGGAAGCA GCAGTTTATC TGATATCTAT 
CAGGCTACCG AAAGTGAGGT AGGAGATGTC GATTTAACAC GTCTTCCAGA AGGACCTGTT 
GATTCTGAAG ATGAAGAAGA GGAAGATGAA GACATTGATC GAACAGATCC TCTGCAAGGG 
CGGGATCTTG TCCGAGAATG TCTTGAAAAA GAACCTGCAG ATAAAACTGA TGATGACATT 
GAACAATTGC TGGAGTTTAT GCACCAGCTT CCCGCTTTTG CAAATATGAC CATGTCTGTA 
AGGAGAGAAC TCTGCTCAGT GATGATTTTT GAAGTGGTAG AGCAGGCTGG AGCTGTCATT 
CTTGAAGATG GGCAAGAGCT TGATTCATGG TATGTTATTT TAAATGGCAC TGTGGAAATC 
AGTCATCCAG ATGGAAAAGT TGACAATCTC TTTATGGGAA ATAGTTTTGG AATCACACCC 
ACTCTGGATA AACAGTACAT GCATGGAGTT GTTAGGACTA AAGTAGATGA CTGTCAGTTT 
GTCTGCATAG CCCAGCAGGA TTATTGGAGA ATTTTAAACC ATGTTGAAAA AAATACCCAT 
AAAGTTGAGG AAGAGGGAGA AATTGTTATG GTACATGAGC ATCGTGAACT GGATCGAAGT 
GGAACCAGGA AAGGACACAT TGTAATTAAG GGAACACCTG AGCGTCTCAT CATGCATTTA 
ATAGAAGAGC ATTCCATTGT GGACCCAACT TATATAGAAG ATTTCCTATT AACTTACAGA 
ACATTTCTTG CAAGTCCTTT GGATGTTGGG ACCAAACTGT TGGAATGGTT TAAAATTGAC 
AGCTTAAGGG ATAAGGTAAC ACGGATTGTA TTATTATGGG TAAATAATCA TTTCAATGAT 
TTTGAAGGTG ATCCTGCTAT GACTCGATTT CTAGAGGAAT TTGAAAAAAA TCTGGAAGAT 
ACAAAAATGA ATGGTCATCT CCGGTTATTG AATATTGCGT GTGCTGCAAA GGCTAAGTGG 
AGACAGGTTG TGCTGCAGAA AGCTTCCCGG GAGTCACCTC TGCATTTCAG CCTAAGTGGA 
GGAAGTGAGA AGGGATTTGG TATTTTTGTT GAAAGCGTAG AACCTAGTAG CAAAGCTGCT 
GATGCAGGAC TGAAACGTGG TGATCAAGTA ATGGAAGTAA ATGGACAAAA TTTTGAGAAT 
ATAACGTTTG TGAAGGCCCT TGAAATTTTG AGGAATAATA CTCATCTTGC ACTTACTGTA 
AAGACCAACA TTTTTGTGTT CAAAGAGTTA CTTAGTAGGA CTGAACAAGA GAAATCTGGT 
GTTCCTCATA TTCCCAAAAT TGCTGAAAAA AAAAGTAATC GCCACTCAAT CCAGGATGTA 
CCAGGAGATC TTGAACAGAC ATCACAGGAG AAAGGAAATA AGAAAATAAA AGCAAATACC 
GTTTCAGGTG GAAGAAACAA AATCAGGAAA ATTTTGGATA AAACACGATT TAGTATCTTG 
CCTCCAAAAC TTTTTAGTGA TGGAGGCCTG AGCCAGTCAC AGGATGACAG CATTGTGGGA 
ACGAGACACT GCAGGCACAG TCTGGCCATC ATGCCTATCC CTGGAACACT CTCGTCCAGC 
AGCCCCGACC TCCTCCAGCC TACCACCAGC ATGTTGGATT TTTCTAATCC CTCAGATATT 
CCTGATCAAG TTATAAGAGT TTTCAAAGCA GATCAGCAAA GTTGCTACAT TATCATCAGT 
AAAGACACTA CAGCTAAGGA AGTTGTCTGT CAAGCTATTC ATGAATTTGG TTTGACTGGT 
GCATCTGACA CATACTCTCT CTGTGAAGTT TCTGTTACTC CTGAGGGTGT TATAAAACAG 
AGAAGACTTC CAGACCAATT CTCCAAATTA GCTGATAGAA TTCAGCTCAA TGGAAGGTAT 
TATTTGAAAA ATAACATGGA AACAGAAACC CTATGTTCAG ATGAAGATGC TCAAGAACTA 
GTTAAGGAAA GCCAGCTGTC CATGCTACAA CTAAGTACTA TTGAGGTGGC CACTCAGCTG 
TCAATGAGGG ACTTTGATTT ATTTCGTAAT ATTGAGCCTA CTGAATATAT TGATGACCTT 
TTTAAGTTAG AGTCCAAAAC AGGAAATACT CACTTGAAAG AATTTGAAGA CATTGTAAAC 
CAAGAGACAT TCTGGGTTGC ATCAGAGATT TTAACTGAAT CAAATCAGCT CAAACGAATG 
AAGATTATTA AACATTTTAT TAAAATTGCA CTTCATTGTC GAGAATGTAA GAACTTCAAT 
TCCATGTTTG CAATAATAAG TGGTTTGAAC TTGGCATCTG TAGCACGACT CAGAGGTACT 
TGGGAAAAGT TACCAAGCAA GTATGATAAA CACTTCCAAG ATCTGCAAGA CCTTTTTGAT 
CCATCCAGAA ACATGGCAAA ATACAGAAAT ATTCTTAGTA GTCAAAGCAT GCAGCCTCCA 
ATTATTCCAC TCTTTCCTGT TGTCAAGAAA GATATGACAT TTCTACATGA AGGAAATGAT 
TCCAAAGTTG ATGGTTTGGT AAATTTCGAG AAACTCAGAA TGATTGCCAA GGAAATTCGT 
CAGGTTGTTC GAATGACTTC TGCTAACATG GATCCAGCTA TGATGTTCCG ACAGAGGAAG 
AAGAGGTGGC GGAGTCTGGG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_024995045.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850813.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

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CloneID	OBa236584
Clone ID Related Accession (Same CDS sequence)	XM_024995045.1
Accession Version	XM_024995045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGTG CCACCAGCAG AGAGGAGGAA ATGGGGAAAG CGAGATCTAG AGAGAGTTCA 
GAAACTATTG CCAGATGTTG GTATATCCTG CTTTCTGGAT CTGTGCTTAT GAAAGACTCC 
ATGGTTTTGC CTCCTTGCAG TTTTGGTAAG CAGTTTGGAG GAAAAAGAGG ATGTGATTGT 
CTTGTATTAG AGCCTTCAGA AATGATTGTG GTAGAGAATT CCAAAGATAA TGAAGATAAT 
ATTCTACAAA GAGAAATTCC TGCCAGACAG TCTCGAAGAA GATTTCGGAA AATTAATTGT 
AAAGGAGAAC GCCAGACTAT TACTGATGAT GTGGATATTA ACAGCTATCT TTCTCTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850813.1
CDS	16..4701
Translation	MLSATSREEEMGKARSRESSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_024995045.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995045.1

ORF Insert Sequence:

ATGCTCAGTG CCACCAGCAG AGAGGAGGAA ATGGGGAAAG CGAGATCTAG AGAGAGTTCA 
GAAACTATTG CCAGATGTTG GTATATCCTG CTTTCTGGAT CTGTGCTTAT GAAAGACTCC 
ATGGTTTTGC CTCCTTGCAG TTTTGGTAAG CAGTTTGGAG GAAAAAGAGG ATGTGATTGT 
CTTGTATTAG AGCCTTCAGA AATGATTGTG GTAGAGAATT CCAAAGATAA TGAAGATAAT 
ATTCTACAAA GAGAAATTCC TGCCAGACAG TCTCGAAGAA GATTTCGGAA AATTAATTGT 
AAAGGAGAAC GCCAGACTAT TACTGATGAT GTGGATATTA ACAGCTATCT TTCTCTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCGA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_015472120.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327606.2 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

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CloneID	OBa236585
Clone ID Related Accession (Same CDS sequence)	XM_015472120.2
Accession Version	XM_015472120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015472120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG 
GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC 
CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG 
AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA 
ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA 
AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT 
TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA 
GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC 
CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC 
CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT 
ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA 
GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG 
TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC 
AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT 
TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC 
TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA 
CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT 
AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT 
GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT 
GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC 
TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA 
GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327606.2
CDS	147..4688
Translation	MLVYPAFWICAYERLHGFASLQVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_015472120.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472120.2

ORF Insert Sequence:

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG 
GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC 
CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG 
AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA 
ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA 
AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT 
TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA 
GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC 
CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC 
CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT 
ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA 
GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG 
TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC 
AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT 
TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC 
TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA 
CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT 
AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT 
GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT 
GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC 
TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA 
GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_005209077.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209134.2 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa236586
Clone ID Related Accession (Same CDS sequence)	XM_005209077.4
Accession Version	XM_005209077.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209077.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209134.2
CDS	193..4728
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_005209077.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209077.4

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCG ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_024995046.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850814.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa236587
Clone ID Related Accession (Same CDS sequence)	XM_024995046.1
Accession Version	XM_024995046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGT TTAAACTCCA CAGACTGTCA GTAATATGGA AAAATTGGAT GTTTAATCAG 
GAATCTGAAC ATGTGTTTCA GGTAGAGAAT TCCAAAGATA ATGAAGATAA TATTCTACAA 
AGAGAAATTC CTGCCAGACA GTCTCGAAGA AGATTTCGGA AAATTAATTG TAAAGGAGAA 
CGCCAGACTA TTACTGATGA TGTGGATATT AACAGCTATC TTTCTCTTCC AGCCGATCTT 
ACCAAGATGC ACATTGCAGA CAACCCTCAT CCACAGGTGA CTCACGTGTC TTCTAGTCAG 
TCTGGCTGTA GCATCGCCAG TGACTCTGGA AGCAGCAGTT TATCTGATAT CTATCAGGCT 
ACCGAAAGTG AGGTAGGAGA TGTCGATTTA ACACGTCTTC CAGAAGGACC TGTTGATTCT 
GAAGATGAAG AAGAGGAAGA TGAAGACATT GATCGAACAG ATCCTCTGCA AGGGCGGGAT 
CTTGTCCGAG AATGTCTTGA AAAAGAACCT GCAGATAAAA CTGATGATGA CATTGAACAA 
TTGCTGGAGT TTATGCACCA GCTTCCCGCT TTTGCAAATA TGACCATGTC TGTAAGGAGA 
GAACTCTGCT CAGTGATGAT TTTTGAAGTG GTAGAGCAGG CTGGAGCTGT CATTCTTGAA 
GATGGGCAAG AGCTTGATTC ATGGTATGTT ATTTTAAATG GCACTGTGGA AATCAGTCAT 
CCAGATGGAA AAGTTGACAA TCTCTTTATG GGAAATAGTT TTGGAATCAC ACCCACTCTG 
GATAAACAGT ACATGCATGG AGTTGTTAGG ACTAAAGTAG ATGACTGTCA GTTTGTCTGC 
ATAGCCCAGC AGGATTATTG GAGAATTTTA AACCATGTTG AAAAAAATAC CCATAAAGTT 
GAGGAAGAGG GAGAAATTGT TATGGTACAT GAGCATCGTG AACTGGATCG AAGTGGAACC 
AGGAAAGGAC ACATTGTAAT TAAGGGAACA CCTGAGCGTC TCATCATGCA TTTAATAGAA 
GAGCATTCCA TTGTGGACCC AACTTATATA GAAGATTTCC TATTAACTTA CAGAACATTT 
CTTGCAAGTC CTTTGGATGT TGGGACCAAA CTGTTGGAAT GGTTTAAAAT TGACAGCTTA 
AGGGATAAGG TAACACGGAT TGTATTATTA TGGGTAAATA ATCATTTCAA TGATTTTGAA 
GGTGATCCTG CTATGACTCG ATTTCTAGAG GAATTTGAAA AAAATCTGGA AGATACAAAA 
ATGAATGGTC ATCTCCGGTT ATTGAATATT GCGTGTGCTG CAAAGGCTAA GTGGAGACAG 
GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA CCTCTGCATT TCAGCCTAAG TGGAGGAAGT 
GAGAAGGGAT TTGGTATTTT TGTTGAAAGC GTAGAACCTA GTAGCAAAGC TGCTGATGCA 
GGACTGAAAC GTGGTGATCA AGTAATGGAA GTAAATGGAC AAAATTTTGA GAATATAACG 
TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT AATACTCATC TTGCACTTAC TGTAAAGACC 
AACATTTTTG TGTTCAAAGA GTTACTTAGT AGGACTGAAC AAGAGAAATC TGGTGTTCCT 
CATATTCCCA AAATTGCTGA AAAAAAAAGT AATCGCCACT CAATCCAGGA TGTACCAGGA 
GATCTTGAAC AGACATCACA GGAGAAAGGA AATAAGAAAA TAAAAGCAAA TACCGTTTCA 
GGTGGAAGAA ACAAAATCAG GAAAATTTTG GATAAAACAC GATTTAGTAT CTTGCCTCCA 
AAACTTTTTA GTGATGGAGG CCTGAGCCAG TCACAGGATG ACAGCATTGT GGGAACGAGA 
CACTGCAGGC ACAGTCTGGC CATCATGCCT ATCCCTGGAA CACTCTCGTC CAGCAGCCCC 
GACCTCCTCC AGCCTACCAC CAGCATGTTG GATTTTTCTA ATCCCTCAGA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAG CAAAGTTGCT ACATTATCAT CAGTAAAGAC 
ACTACAGCTA AGGAAGTTGT CTGTCAAGCT ATTCATGAAT TTGGTTTGAC TGGTGCATCT 
GACACATACT CTCTCTGTGA AGTTTCTGTT ACTCCTGAGG GTGTTATAAA ACAGAGAAGA 
CTTCCAGACC AATTCTCCAA ATTAGCTGAT AGAATTCAGC TCAATGGAAG GTATTATTTG 
AAAAATAACA TGGAAACAGA AACCCTATGT TCAGATGAAG ATGCTCAAGA ACTAGTTAAG 
GAAAGCCAGC TGTCCATGCT ACAACTAAGT ACTATTGAGG TGGCCACTCA GCTGTCAATG 
AGGGACTTTG ATTTATTTCG TAATATTGAG CCTACTGAAT ATATTGATGA CCTTTTTAAG 
TTAGAGTCCA AAACAGGAAA TACTCACTTG AAAGAATTTG AAGACATTGT AAACCAAGAG 
ACATTCTGGG TTGCATCAGA GATTTTAACT GAATCAAATC AGCTCAAACG AATGAAGATT 
ATTAAACATT TTATTAAAAT TGCACTTCAT TGTCGAGAAT GTAAGAACTT CAATTCCATG 
TTTGCAATAA TAAGTGGTTT GAACTTGGCA TCTGTAGCAC GACTCAGAGG TACTTGGGAA 
AAGTTACCAA GCAAGTATGA TAAACACTTC CAAGATCTGC AAGACCTTTT TGATCCATCC 
AGAAACATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTTC CTGTTGTCAA GAAAGATATG ACATTTCTAC ATGAAGGAAA TGATTCCAAA 
GTTGATGGTT TGGTAAATTT CGAGAAACTC AGAATGATTG CCAAGGAAAT TCGTCAGGTT 
GTTCGAATGA CTTCTGCTAA CATGGATCCA GCTATGATGT TCCGACAGAG GTCACTGAGT 
CAAGGCAGCA CAAATTCAAA CATGCTGGAT GTTCAGGGAG GTGCTCACAA AAAAAGGGCA 
CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG CTATATGAAG ATGCCCAAAT GGCAAGAAAA 
GTGAAGCAGT ATCTTTCCAG TCTGGATGTC GAGACAGATG AAGAGAAGTT CCAGATGATG 
TCATTGCAAT GGGAGCCTGC ATATGGTACC TTGACCAAGA ATTTAAATGA GAAAAAATCA 
ATGAAGTCAT CAGAAATGTC TCCGGTGCCA CTGAGGTCAG CTGGCCAAAC AACTAAAGCT 
CATTTGCATC AGCCTCACAG AGGAAGCCAG GTGCTTCAGG TGCCAGCTGT AAATTTGCAC 
CCTATCAGGA AGAAGGGCCA AGGAAAAGAC CCTGCCACCC TGAGTACAGG TTTACCTCAG 
AAAATTTTAG GAACAGCTGA AGAAGTAAGT GGTGGTAAGA AACACACAGA AGACACCGTT 
TCTGTGGCAT CATCCTTACA TTCCAGCCCT CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA 
GGTTATACAC TTATACCATC AGCTAAATCT GACAACTTGT CTGACTCCAG CCACAGTGAG 
ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT TGTTCTGTTG ACTCCATGTC TACAGCTCTA 
CAGGATGAAC GGTGTTCCTC CCAGGCTGTG GCAGTCCCTG AATCCACTGG AGCATTGGAA 
AAGACAGAGC ACTCCTCAGG GATAGGAGAC CATAGTCAGC TTGGCCCTGG GTGGACACTC 
TCAAAGCCAT CTCTAATCAA CAAGAGTTTA GCTGTCTCAT CTTCTGTGAG CAATGAAGAG 
ATTTCTCATG AGCACATCAT TATAGAAACA GCTGATAGTG GTCGTGGAAG TTGGACTTCC 
TGCTCAAGCA GTTCCCATGA CAACTTCCAA AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT 
TTGAACTCTT ACCGACCTAC CCATTTGGAT GGTCCCATAG CTGAAGTTGA ACCCACTGAC 
TGTGAGCACT ACAGCTGTCC CAAAGGCTGC TCTCGAACTT GTGGTCAGTG TAAAGGAAGC 
CTAGAGACTA ATCAGATGAG ACAGAGTTGG GCTTCCTCTA GTTCTCTGTC TGACACATAC 
GAACCAAACT ATGGGACAGT TAAACGGAGA GTGCTGGAGA ACACCCCAGC TGAATCATCT 
GAAGGCTTGG ACCACAAAGA GGGCATCGAC CCAGTCTATA AAACTGTTAC TTCAAGTACA 
GAAAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AGGAGGATAG GTATAGGGAG 
CCACCTCCCA CTCCTCCAGG ATACATGGGG ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCTCCACC TAAAACCTCC AGAGTATAGT GTGGCGGTGC AGAGGTCAAA GATGATGCAT 
AACAGCCTCT CTAGACTGCC ACCAGCTTCT CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA 
TCGAAGATTG TAATTCAGCC TCAGAGGCAT AAACTAGATG TGACTGATGG AGATAGCGAA 
GCAGATGAAA ATGAACAGGT TTCAGCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850814.1
CDS	98..4630
Translation	MSLFKLHRLSVIWKNWMFNQESEHVFQVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_024995046.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995046.1

ORF Insert Sequence:

ATGAGCCTGT TTAAACTCCA CAGACTGTCA GTAATATGGA AAAATTGGAT GTTTAATCAG 
GAATCTGAAC ATGTGTTTCA GGTAGAGAAT TCCAAAGATA ATGAAGATAA TATTCTACAA 
AGAGAAATTC CTGCCAGACA GTCTCGAAGA AGATTTCGGA AAATTAATTG TAAAGGAGAA 
CGCCAGACTA TTACTGATGA TGTGGATATT AACAGCTATC TTTCTCTTCC AGCCGATCTT 
ACCAAGATGC ACATTGCAGA CAACCCTCAT CCACAGGTGA CTCACGTGTC TTCTAGTCAG 
TCTGGCTGTA GCATCGCCAG TGACTCTGGA AGCAGCAGTT TATCTGATAT CTATCAGGCT 
ACCGAAAGTG AGGTAGGAGA TGTCGATTTA ACACGTCTTC CAGAAGGACC TGTTGATTCT 
GAAGATGAAG AAGAGGAAGA TGAAGACATT GATCGAACAG ATCCTCTGCA AGGGCGGGAT 
CTTGTCCGAG AATGTCTTGA AAAAGAACCT GCAGATAAAA CTGATGATGA CATTGAACAA 
TTGCTGGAGT TTATGCACCA GCTTCCCGCT TTTGCAAATA TGACCATGTC TGTAAGGAGA 
GAACTCTGCT CAGTGATGAT TTTTGAAGTG GTAGAGCAGG CTGGAGCTGT CATTCTTGAA 
GATGGGCAAG AGCTTGATTC ATGGTATGTT ATTTTAAATG GCACTGTGGA AATCAGTCAT 
CCAGATGGAA AAGTTGACAA TCTCTTTATG GGAAATAGTT TTGGAATCAC ACCCACTCTG 
GATAAACAGT ACATGCATGG AGTTGTTAGG ACTAAAGTAG ATGACTGTCA GTTTGTCTGC 
ATAGCCCAGC AGGATTATTG GAGAATTTTA AACCATGTTG AAAAAAATAC CCATAAAGTT 
GAGGAAGAGG GAGAAATTGT TATGGTACAT GAGCATCGTG AACTGGATCG AAGTGGAACC 
AGGAAAGGAC ACATTGTAAT TAAGGGAACA CCTGAGCGTC TCATCATGCA TTTAATAGAA 
GAGCATTCCA TTGTGGACCC AACTTATATA GAAGATTTCC TATTAACTTA CAGAACATTT 
CTTGCAAGTC CTTTGGATGT TGGGACCAAA CTGTTGGAAT GGTTTAAAAT TGACAGCTTA 
AGGGATAAGG TAACACGGAT TGTATTATTA TGGGTAAATA ATCATTTCAA TGATTTTGAA 
GGTGATCCTG CTATGACTCG ATTTCTAGAG GAATTTGAAA AAAATCTGGA AGATACAAAA 
ATGAATGGTC ATCTCCGGTT ATTGAATATT GCGTGTGCTG CAAAGGCTAA GTGGAGACAG 
GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA CCTCTGCATT TCAGCCTAAG TGGAGGAAGT 
GAGAAGGGAT TTGGTATTTT TGTTGAAAGC GTAGAACCTA GTAGCAAAGC TGCTGATGCA 
GGACTGAAAC GTGGTGATCA AGTAATGGAA GTAAATGGAC AAAATTTTGA GAATATAACG 
TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT AATACTCATC TTGCACTTAC TGTAAAGACC 
AACATTTTTG TGTTCAAAGA GTTACTTAGT AGGACTGAAC AAGAGAAATC TGGTGTTCCT 
CATATTCCCA AAATTGCTGA AAAAAAAAGT AATCGCCACT CAATCCAGGA TGTACCAGGA 
GATCTTGAAC AGACATCACA GGAGAAAGGA AATAAGAAAA TAAAAGCAAA TACCGTTTCA 
GGTGGAAGAA ACAAAATCAG GAAAATTTTG GATAAAACAC GATTTAGTAT CTTGCCTCCA 
AAACTTTTTA GTGATGGAGG CCTGAGCCAG TCACAGGATG ACAGCATTGT GGGAACGAGA 
CACTGCAGGC ACAGTCTGGC CATCATGCCT ATCCCTGGAA CACTCTCGTC CAGCAGCCCC 
GACCTCCTCC AGCCTACCAC CAGCATGTTG GATTTTTCTA ATCCCTCAGA TATTCCTGAT 
CAAGTTATAA GAGTTTTCAA AGCAGATCAG CAAAGTTGCT ACATTATCAT CAGTAAAGAC 
ACTACAGCTA AGGAAGTTGT CTGTCAAGCT ATTCATGAAT TTGGTTTGAC TGGTGCATCT 
GACACATACT CTCTCTGTGA AGTTTCTGTT ACTCCTGAGG GTGTTATAAA ACAGAGAAGA 
CTTCCAGACC AATTCTCCAA ATTAGCTGAT AGAATTCAGC TCAATGGAAG GTATTATTTG 
AAAAATAACA TGGAAACAGA AACCCTATGT TCAGATGAAG ATGCTCAAGA ACTAGTTAAG 
GAAAGCCAGC TGTCCATGCT ACAACTAAGT ACTATTGAGG TGGCCACTCA GCTGTCAATG 
AGGGACTTTG ATTTATTTCG TAATATTGAG CCTACTGAAT ATATTGATGA CCTTTTTAAG 
TTAGAGTCCA AAACAGGAAA TACTCACTTG AAAGAATTTG AAGACATTGT AAACCAAGAG 
ACATTCTGGG TTGCATCAGA GATTTTAACT GAATCAAATC AGCTCAAACG AATGAAGATT 
ATTAAACATT TTATTAAAAT TGCACTTCAT TGTCGAGAAT GTAAGAACTT CAATTCCATG 
TTTGCAATAA TAAGTGGTTT GAACTTGGCA TCTGTAGCAC GACTCAGAGG TACTTGGGAA 
AAGTTACCAA GCAAGTATGA TAAACACTTC CAAGATCTGC AAGACCTTTT TGATCCATCC 
AGAAACATGG CAAAATACAG AAATATTCTT AGTAGTCAAA GCATGCAGCC TCCAATTATT 
CCACTCTTTC CTGTTGTCAA GAAAGATATG ACATTTCTAC ATGAAGGAAA TGATTCCAAA 
GTTGATGGTT TGGTAAATTT CGAGAAACTC AGAATGATTG CCAAGGAAAT TCGTCAGGTT 
GTTCGAATGA CTTCTGCTAA CATGGATCCA GCTATGATGT TCCGACAGAG GTCACTGAGT 
CAAGGCAGCA CAAATTCAAA CATGCTGGAT GTTCAGGGAG GTGCTCACAA AAAAAGGGCA 
CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG CTATATGAAG ATGCCCAAAT GGCAAGAAAA 
GTGAAGCAGT ATCTTTCCAG TCTGGATGTC GAGACAGATG AAGAGAAGTT CCAGATGATG 
TCATTGCAAT GGGAGCCTGC ATATGGTACC TTGACCAAGA ATTTAAATGA GAAAAAATCA 
ATGAAGTCAT CAGAAATGTC TCCGGTGCCA CTGAGGTCAG CTGGCCAAAC AACTAAAGCT 
CATTTGCATC AGCCTCACAG AGGAAGCCAG GTGCTTCAGG TGCCAGCTGT AAATTTGCAC 
CCTATCAGGA AGAAGGGCCA AGGAAAAGAC CCTGCCACCC TGAGTACAGG TTTACCTCAG 
AAAATTTTAG GAACAGCTGA AGAAGTAAGT GGTGGTAAGA AACACACAGA AGACACCGTT 
TCTGTGGCAT CATCCTTACA TTCCAGCCCT CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA 
GGTTATACAC TTATACCATC AGCTAAATCT GACAACTTGT CTGACTCCAG CCACAGTGAG 
ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT TGTTCTGTTG ACTCCATGTC TACAGCTCTA 
CAGGATGAAC GGTGTTCCTC CCAGGCTGTG GCAGTCCCTG AATCCACTGG AGCATTGGAA 
AAGACAGAGC ACTCCTCAGG GATAGGAGAC CATAGTCAGC TTGGCCCTGG GTGGACACTC 
TCAAAGCCAT CTCTAATCAA CAAGAGTTTA GCTGTCTCAT CTTCTGTGAG CAATGAAGAG 
ATTTCTCATG AGCACATCAT TATAGAAACA GCTGATAGTG GTCGTGGAAG TTGGACTTCC 
TGCTCAAGCA GTTCCCATGA CAACTTCCAA AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT 
TTGAACTCTT ACCGACCTAC CCATTTGGAT GGTCCCATAG CTGAAGTTGA ACCCACTGAC 
TGTGAGCACT ACAGCTGTCC CAAAGGCTGC TCTCGAACTT GTGGTCAGTG TAAAGGAAGC 
CTAGAGACTA ATCAGATGAG ACAGAGTTGG GCTTCCTCTA GTTCTCTGTC TGACACATAC 
GAACCAAACT ATGGGACAGT TAAACGGAGA GTGCTGGAGA ACACCCCAGC TGAATCATCT 
GAAGGCTTGG ACCACAAAGA GGGCATCGAC CCAGTCTATA AAACTGTTAC TTCAAGTACA 
GAAAAGGGCT TGATTGTGTA CTGTGTCACC TCACCCAAGA AGGAGGATAG GTATAGGGAG 
CCACCTCCCA CTCCTCCAGG ATACATGGGG ATTTCTTTAG CGGACCTAAA GGAAGGACCC 
CACCTCCACC TAAAACCTCC AGAGTATAGT GTGGCGGTGC AGAGGTCAAA GATGATGCAT 
AACAGCCTCT CTAGACTGCC ACCAGCTTCT CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA 
TCGAAGATTG TAATTCAGCC TCAGAGGCAT AAACTAGATG TGACTGATGG AGATAGCGAA 
GCAGATGAAA ATGAACAGGT TTCAGCAGTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_024995047.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024850815.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA.

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CloneID	OBa236588
Clone ID Related Accession (Same CDS sequence)	XM_024995047.1
Accession Version	XM_024995047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGTCAC TGAGTCAAGG CAGCACAAAT 
TCAAACATGC TGGATGTTCA GGGAGGTGCT CACAAAAAAA GGGCACGCCG TAGCTCTCTG 
CTAAATGCCA AGAAGCTATA TGAAGATGCC CAAATGGCAA GAAAAGTGAA GCAGTATCTT 
TCCAGTCTGG ATGTCGAGAC AGATGAAGAG AAGTTCCAGA TGATGTCATT GCAATGGGAG 
CCTGCATATG GTACCTTGAC CAAGAATTTA AATGAGAAAA AATCAATGAA GTCATCAGAA 
ATGTCTCCGG TGCCACTGAG GTCAGCTGGC CAAACAACTA AAGCTCATTT GCATCAGCCT 
CACAGAGGAA GCCAGGTGCT TCAGGTGCCA GCTGTAAATT TGCACCCTAT CAGGAAGAAG 
GGCCAAGGAA AAGACCCTGC CACCCTGAGT ACAGGTTTAC CTCAGAAAAT TTTAGGAACA 
GCTGAAGAAG TAAGTGGTGG TAAGAAACAC ACAGAAGACA CCGTTTCTGT GGCATCATCC 
TTACATTCCA GCCCTCCTGT GTCTCCTCAA GGTTCCCCCC GCAAAGGTTA TACACTTATA 
CCATCAGCTA AATCTGACAA CTTGTCTGAC TCCAGCCACA GTGAGATTTC TTCACGGTCC 
AGCATTGTGA GCAATTGTTC TGTTGACTCC ATGTCTACAG CTCTACAGGA TGAACGGTGT 
TCCTCCCAGG CTGTGGCAGT CCCTGAATCC ACTGGAGCAT TGGAAAAGAC AGAGCACTCC 
TCAGGGATAG GAGACCATAG TCAGCTTGGC CCTGGGTGGA CACTCTCAAA GCCATCTCTA 
ATCAACAAGA GTTTAGCTGT CTCATCTTCT GTGAGCAATG AAGAGATTTC TCATGAGCAC 
ATCATTATAG AAACAGCTGA TAGTGGTCGT GGAAGTTGGA CTTCCTGCTC AAGCAGTTCC 
CATGACAACT TCCAAAGTCT TCCAAACCCC AAAGGCTGGG AGTTTTTGAA CTCTTACCGA 
CCTACCCATT TGGATGGTCC CATAGCTGAA GTTGAACCCA CTGACTGTGA GCACTACAGC 
TGTCCCAAAG GCTGCTCTCG AACTTGTGGT CAGTGTAAAG GAAGCCTAGA GACTAATCAG 
ATGAGACAGA GTTGGGCTTC CTCTAGTTCT CTGTCTGACA CATACGAACC AAACTATGGG 
ACAGTTAAAC GGAGAGTGCT GGAGAACACC CCAGCTGAAT CATCTGAAGG CTTGGACCAC 
AAAGAGGGCA TCGACCCAGT CTATAAAACT GTTACTTCAA GTACAGAAAA GGGCTTGATT 
GTGTACTGTG TCACCTCACC CAAGAAGGAG GATAGGTATA GGGAGCCACC TCCCACTCCT 
CCAGGATACA TGGGGATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCT CCACCTAAAA 
CCTCCAGAGT ATAGTGTGGC GGTGCAGAGG TCAAAGATGA TGCATAACAG CCTCTCTAGA 
CTGCCACCAG CTTCTCTCAG TAGCAGCCCT GTGGCCTGTG TTCCATCGAA GATTGTAATT 
CAGCCTCAGA GGCATAAACT AGATGTGACT GATGGAGATA GCGAAGCAGA TGAAAATGAA 
CAGGTTTCAG CAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024850815.1
CDS	451..4968
Translation	MLVYPAFWICAYERLHGFASLQVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_024995047.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995047.1

ORF Insert Sequence:

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCGATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGTCAC TGAGTCAAGG CAGCACAAAT 
TCAAACATGC TGGATGTTCA GGGAGGTGCT CACAAAAAAA GGGCACGCCG TAGCTCTCTG 
CTAAATGCCA AGAAGCTATA TGAAGATGCC CAAATGGCAA GAAAAGTGAA GCAGTATCTT 
TCCAGTCTGG ATGTCGAGAC AGATGAAGAG AAGTTCCAGA TGATGTCATT GCAATGGGAG 
CCTGCATATG GTACCTTGAC CAAGAATTTA AATGAGAAAA AATCAATGAA GTCATCAGAA 
ATGTCTCCGG TGCCACTGAG GTCAGCTGGC CAAACAACTA AAGCTCATTT GCATCAGCCT 
CACAGAGGAA GCCAGGTGCT TCAGGTGCCA GCTGTAAATT TGCACCCTAT CAGGAAGAAG 
GGCCAAGGAA AAGACCCTGC CACCCTGAGT ACAGGTTTAC CTCAGAAAAT TTTAGGAACA 
GCTGAAGAAG TAAGTGGTGG TAAGAAACAC ACAGAAGACA CCGTTTCTGT GGCATCATCC 
TTACATTCCA GCCCTCCTGT GTCTCCTCAA GGTTCCCCCC GCAAAGGTTA TACACTTATA 
CCATCAGCTA AATCTGACAA CTTGTCTGAC TCCAGCCACA GTGAGATTTC TTCACGGTCC 
AGCATTGTGA GCAATTGTTC TGTTGACTCC ATGTCTACAG CTCTACAGGA TGAACGGTGT 
TCCTCCCAGG CTGTGGCAGT CCCTGAATCC ACTGGAGCAT TGGAAAAGAC AGAGCACTCC 
TCAGGGATAG GAGACCATAG TCAGCTTGGC CCTGGGTGGA CACTCTCAAA GCCATCTCTA 
ATCAACAAGA GTTTAGCTGT CTCATCTTCT GTGAGCAATG AAGAGATTTC TCATGAGCAC 
ATCATTATAG AAACAGCTGA TAGTGGTCGT GGAAGTTGGA CTTCCTGCTC AAGCAGTTCC 
CATGACAACT TCCAAAGTCT TCCAAACCCC AAAGGCTGGG AGTTTTTGAA CTCTTACCGA 
CCTACCCATT TGGATGGTCC CATAGCTGAA GTTGAACCCA CTGACTGTGA GCACTACAGC 
TGTCCCAAAG GCTGCTCTCG AACTTGTGGT CAGTGTAAAG GAAGCCTAGA GACTAATCAG 
ATGAGACAGA GTTGGGCTTC CTCTAGTTCT CTGTCTGACA CATACGAACC AAACTATGGG 
ACAGTTAAAC GGAGAGTGCT GGAGAACACC CCAGCTGAAT CATCTGAAGG CTTGGACCAC 
AAAGAGGGCA TCGACCCAGT CTATAAAACT GTTACTTCAA GTACAGAAAA GGGCTTGATT 
GTGTACTGTG TCACCTCACC CAAGAAGGAG GATAGGTATA GGGAGCCACC TCCCACTCCT 
CCAGGATACA TGGGGATTTC TTTAGCGGAC CTAAAGGAAG GACCCCACCT CCACCTAAAA 
CCTCCAGAGT ATAGTGTGGC GGTGCAGAGG TCAAAGATGA TGCATAACAG CCTCTCTAGA 
CTGCCACCAG CTTCTCTCAG TAGCAGCCCT GTGGCCTGTG TTCCATCGAA GATTGTAATT 
CAGCCTCAGA GGCATAAACT AGATGTGACT GATGGAGATA GCGAAGCAGA TGAAAATGAA 
CAGGTTTCAG CAGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_015472120.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327606.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

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CloneID	OBa90291
Clone ID Related Accession (Same CDS sequence)	XM_015472120.1 , XM_015464187.1
Accession Version	XM_015472120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG 
GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC 
CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG 
AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA 
ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA 
AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT 
TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA 
GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC 
CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC 
CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT 
ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA 
GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG 
TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC 
AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT 
TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC 
TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA 
CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT 
AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT 
GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT 
GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC 
TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA 
GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327606.1
CDS	128..4669
Translation	MLVYPAFWICAYERLHGFASLQVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_015472120.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472120.1

ORF Insert Sequence:

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG 
GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC 
CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG 
AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA 
ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA 
AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT 
TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA 
GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC 
CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC 
CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT 
ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA 
GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG 
TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC 
AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT 
TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC 
TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA 
CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT 
AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT 
GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT 
GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC 
TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA 
GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_015464187.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319673.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

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CloneID	OBa90291
Clone ID Related Accession (Same CDS sequence)	XM_015472120.1 , XM_015464187.1
Accession Version	XM_015464187.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG 
GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC 
CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG 
AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA 
ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA 
AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT 
TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA 
GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC 
CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC 
CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT 
ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA 
GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG 
TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC 
AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT 
TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC 
TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA 
CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT 
AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT 
GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT 
GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC 
TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA 
GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319673.1
CDS	128..4669
Translation	MLVYPAFWICAYERLHGFASLQVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_015464187.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464187.1

ORF Insert Sequence:

ATGTTGGTAT ATCCTGCTTT CTGGATCTGT GCTTATGAAA GACTCCATGG TTTTGCCTCC 
TTGCAGGTAG AGAATTCCAA AGATAATGAA GATAATATTC TACAAAGAGA AATTCCTGCC 
AGACAGTCTC GAAGAAGATT TCGGAAAATT AATTGTAAAG GAGAACGCCA GACTATTACT 
GATGATGTGG ATATTAACAG CTATCTTTCT CTTCCAGCCG ATCTTACCAA GATGCACATT 
GCAGACAACC CTCATCCACA GGTGACTCAC GTGTCTTCTA GTCAGTCTGG CTGTAGCATC 
GCCAGTGACT CTGGAAGCAG CAGTTTATCT GATATCTATC AGGCTACCGA AAGTGAGGTA 
GGAGATGTCG ATTTAACACG TCTTCCAGAA GGACCTGTTG ATTCTGAAGA TGAAGAAGAG 
GAAGATGAAG ACATTGATCG AACAGATCCT CTGCAAGGGC GGGATCTTGT CCGAGAATGT 
CTTGAAAAAG AACCTGCAGA TAAAACTGAT GATGACATTG AACAATTGCT GGAGTTTATG 
CACCAGCTTC CCGCTTTTGC AAATATGACC ATGTCTGTAA GGAGAGAACT CTGCTCAGTG 
ATGATTTTTG AAGTGGTAGA GCAGGCTGGA GCTGTCATTC TTGAAGATGG GCAAGAGCTT 
GATTCATGGT ATGTTATTTT AAATGGCACT GTGGAAATCA GTCATCCAGA TGGAAAAGTT 
GACAATCTCT TTATGGGAAA TAGTTTTGGA ATCACACCCA CTCTGGATAA ACAGTACATG 
CATGGAGTTG TTAGGACTAA AGTAGATGAC TGTCAGTTTG TCTGCATAGC CCAGCAGGAT 
TATTGGAGAA TTTTAAACCA TGTTGAAAAA AATACCCATA AAGTTGAGGA AGAGGGAGAA 
ATTGTTATGG TACATGAGCA TCGTGAACTG GATCGAAGTG GAACCAGGAA AGGACACATT 
GTAATTAAGG GAACACCTGA GCGTCTCATC ATGCATTTAA TAGAAGAGCA TTCCATTGTG 
GACCCAACTT ATATAGAAGA TTTCCTATTA ACTTACAGAA CATTTCTTGC AAGTCCTTTG 
GATGTTGGGA CCAAACTGTT GGAATGGTTT AAAATTGACA GCTTAAGGGA TAAGGTAACA 
CGGATTGTAT TATTATGGGT AAATAATCAT TTCAATGATT TTGAAGGTGA TCCTGCTATG 
ACTCAATTTC TAGAGGAATT TGAAAAAAAT CTGGAAGATA CAAAAATGAA TGGTCATCTC 
CGGTTATTGA ATATTGCGTG TGCTGCAAAG GCTAAGTGGA GACAGGTTGT GCTGCAGAAA 
GCTTCCCGGG AGTCACCTCT GCATTTCAGC CTAAGTGGAG GAAGTGAGAA GGGATTTGGT 
ATTTTTGTTG AAAGCGTAGA ACCTAGTAGC AAAGCTGCTG ATGCAGGACT GAAACGTGGT 
GATCAAGTAA TGGAAGTAAA TGGACAAAAT TTTGAGAATA TAACGTTTGT GAAGGCCCTT 
GAAATTTTGA GGAATAATAC TCATCTTGCA CTTACTGTAA AGACCAACAT TTTTGTGTTC 
AAAGAGTTAC TTAGTAGGAC TGAACAAGAG AAATCTGGTG TTCCTCATAT TCCCAAAATT 
GCTGAAAAAA AAAGTAATCG CCACTCAATC CAGGATGTAC CAGGAGATCT TGAACAGACA 
TCACAGGAGA AAGGAAATAA GAAAATAAAA GCAAATACCG TTTCAGGTGG AAGAAACAAA 
ATCAGGAAAA TTTTGGATAA AACACGATTT AGTATCTTGC CTCCAAAACT TTTTAGTGAT 
GGAGGCCTGA GCCAGTCACA GGATGACAGC ATTGTGGGAA CGAGACACTG CAGGCACAGT 
CTGGCCATCA TGCCTATCCC TGGAACACTC TCGTCCAGCA GCCCCGACCT CCTCCAGCCT 
ACCACCAGCA TGTTGGATTT TTCTAATCCC TCAGATATTC CTGATCAAGT TATAAGAGTT 
TTCAAAGCAG ATCAGCAAAG TTGCTACATT ATCATCAGTA AAGACACTAC AGCTAAGGAA 
GTTGTCTGTC AAGCTATTCA TGAATTTGGT TTGACTGGTG CATCTGACAC ATACTCTCTC 
TGTGAAGTTT CTGTTACTCC TGAGGGTGTT ATAAAACAGA GAAGACTTCC AGACCAATTC 
TCCAAATTAG CTGATAGAAT TCAGCTCAAT GGAAGGTATT ATTTGAAAAA TAACATGGAA 
ACAGAAACCC TATGTTCAGA TGAAGATGCT CAAGAACTAG TTAAGGAAAG CCAGCTGTCC 
ATGCTACAAC TAAGTACTAT TGAGGTGGCC ACTCAGCTGT CAATGAGGGA CTTTGATTTA 
TTTCGTAATA TTGAGCCTAC TGAATATATT GATGACCTTT TTAAGTTAGA GTCCAAAACA 
GGAAATACTC ACTTGAAAGA ATTTGAAGAC ATTGTAAACC AAGAGACATT CTGGGTTGCA 
TCAGAGATTT TAACTGAATC AAATCAGCTC AAACGAATGA AGATTATTAA ACATTTTATT 
AAAATTGCAC TTCATTGTCG AGAATGTAAG AACTTCAATT CCATGTTTGC AATAATAAGT 
GGTTTGAACT TGGCATCTGT AGCACGACTC AGAGGTACTT GGGAAAAGTT ACCAAGCAAG 
TATGATAAAC ACTTCCAAGA TCTGCAAGAC CTTTTTGATC CATCCAGAAA CATGGCAAAA 
TACAGAAATA TTCTTAGTAG TCAAAGCATG CAGCCTCCAA TTATTCCACT CTTTCCTGTT 
GTCAAGAAAG ATATGACATT TCTACATGAA GGAAATGATT CCAAAGTTGA TGGTTTGGTA 
AATTTCGAGA AACTCAGAAT GATTGCCAAG GAAATTCGTC AGGTTGTTCG AATGACTTCT 
GCTAACATGG ATCCAGCTAT GATGTTCCGA CAGAGGAAGA AGAGGTGGCG GAGTCTGGGG 
TCACTGAGTC AAGGCAGCAC AAATTCAAAC ATGCTGGATG TTCAGGGAGG TGCTCACAAA 
AAAAGGGCAC GCCGTAGCTC TCTGCTAAAT GCCAAGAAGC TATATGAAGA TGCCCAAATG 
GCAAGAAAAG TGAAGCAGTA TCTTTCCAGT CTGGATGTCG AGACAGATGA AGAGAAGTTC 
CAGATGATGT CATTGCAATG GGAGCCTGCA TATGGTACCT TGACCAAGAA TTTAAATGAG 
AAAAAATCAA TGAAGTCATC AGAAATGTCT CCGGTGCCAC TGAGGTCAGC TGGCCAAACA 
ACTAAAGCTC ATTTGCATCA GCCTCACAGA GGAAGCCAGG TGCTTCAGGT GCCAGCTGTA 
AATTTGCACC CTATCAGGAA GAAGGGCCAA GGAAAAGACC CTGCCACCCT GAGTACAGGT 
TTACCTCAGA AAATTTTAGG AACAGCTGAA GAAGTAAGTG GTGGTAAGAA ACACACAGAA 
GACACCGTTT CTGTGGCATC ATCCTTACAT TCCAGCCCTC CTGTGTCTCC TCAAGGTTCC 
CCCCGCAAAG GTTATACACT TATACCATCA GCTAAATCTG ACAACTTGTC TGACTCCAGC 
CACAGTGAGA TTTCTTCACG GTCCAGCATT GTGAGCAATT GTTCTGTTGA CTCCATGTCT 
ACAGCTCTAC AGGATGAACG GTGTTCCTCC CAGGCTGTGG CAGTCCCTGA ATCCACTGGA 
GCATTGGAAA AGACAGAGCA CTCCTCAGGG ATAGGAGACC ATAGTCAGCT TGGCCCTGGG 
TGGACACTCT CAAAGCCATC TCTAATCAAC AAGAGTTTAG CTGTCTCATC TTCTGTGAGC 
AATGAAGAGA TTTCTCATGA GCACATCATT ATAGAAACAG CTGATAGTGG TCGTGGAAGT 
TGGACTTCCT GCTCAAGCAG TTCCCATGAC AACTTCCAAA GTCTTCCAAA CCCCAAAGGC 
TGGGAGTTTT TGAACTCTTA CCGACCTACC CATTTGGATG GTCCCATAGC TGAAGTTGAA 
CCCACTGACT GTGAGCACTA CAGCTGTCCC AAAGGCTGCT CTCGAACTTG TGGTCAGTGT 
AAAGGAAGCC TAGAGACTAA TCAGATGAGA CAGAGTTGGG CTTCCTCTAG TTCTCTGTCT 
GACACATACG AACCAAACTA TGGGACAGTT AAACGGAGAG TGCTGGAGAA CACCCCAGCT 
GAATCATCTG AAGGCTTGGA CCACAAAGAG GGCATCGACC CAGTCTATAA AACTGTTACT 
TCAAGTACAG AAAAGGGCTT GATTGTGTAC TGTGTCACCT CACCCAAGAA GGAGGATAGG 
TATAGGGAGC CACCTCCCAC TCCTCCAGGA TACATGGGGA TTTCTTTAGC GGACCTAAAG 
GAAGGACCCC ACCTCCACCT AAAACCTCCA GAGTATAGTG TGGCGGTGCA GAGGTCAAAG 
ATGATGCATA ACAGCCTCTC TAGACTGCCA CCAGCTTCTC TCAGTAGCAG CCCTGTGGCC 
TGTGTTCCAT CGAAGATTGT AATTCAGCCT CAGAGGCATA AACTAGATGT GACTGATGGA 
GATAGCGAAG CAGATGAAAA TGAACAGGTT TCAGCAGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_015464185.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319671.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

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CloneID	OBa21248
Clone ID Related Accession (Same CDS sequence)	XM_015464185.1 , XM_003586275.4
Accession Version	XM_015464185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT 
CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC 
CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC 
CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG 
ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT 
GAAAATGAAC AGGTTTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319671.1
CDS	224..5050
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_015464185.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464185.1

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT 
CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC 
CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC 
CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG 
ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT 
GAAAATGAAC AGGTTTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_003586275.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003586323.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21248
Clone ID Related Accession (Same CDS sequence)	XM_015464185.1 , XM_003586275.4
Accession Version	XM_003586275.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586275.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT 
CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC 
CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC 
CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG 
ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT 
GAAAATGAAC AGGTTTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003586323.1
CDS	224..5050
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_003586275.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003586275.4

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG TGTACTGTGT CACCTCACCC AAGAAGGAGG ATAGGTATAG GGAGCCACCT 
CCCACTCCTC CAGGATACAT GGGGATTTCT TTAGCGGACC TAAAGGAAGG ACCCCACCTC 
CACCTAAAAC CTCCAGAGTA TAGTGTGGCG GTGCAGAGGT CAAAGATGAT GCATAACAGC 
CTCTCTAGAC TGCCACCAGC TTCTCTCAGT AGCAGCCCTG TGGCCTGTGT TCCATCGAAG 
ATTGTAATTC AGCCTCAGAG GCATAAACTA GATGTGACTG ATGGAGATAG CGAAGCAGAT 
GAAAATGAAC AGGTTTCAGC AGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_015464186.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319672.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

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CloneID	OBa21249
Clone ID Related Accession (Same CDS sequence)	XM_015464186.1 , XM_002688965.5
Accession Version	XM_015464186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319672.1
CDS	223..5025
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_015464186.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464186.1

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_002688965.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002689011.2 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

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CloneID	OBa21249
Clone ID Related Accession (Same CDS sequence)	XM_015464186.1 , XM_002688965.5
Accession Version	XM_002688965.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002688965.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002689011.2
CDS	223..5025
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_002688965.5
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002688965.5

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GTCACTGAGT CAAGGCAGCA CAAATTCAAA CATGCTGGAT 
GTTCAGGGAG GTGCTCACAA AAAAAGGGCA CGCCGTAGCT CTCTGCTAAA TGCCAAGAAG 
CTATATGAAG ATGCCCAAAT GGCAAGAAAA GTGAAGCAGT ATCTTTCCAG TCTGGATGTC 
GAGACAGATG AAGAGAAGTT CCAGATGATG TCATTGCAAT GGGAGCCTGC ATATGGTACC 
TTGACCAAGA ATTTAAATGA GAAAAAATCA ATGAAGTCAT CAGAAATGTC TCCGGTGCCA 
CTGAGGTCAG CTGGCCAAAC AACTAAAGCT CATTTGCATC AGCCTCACAG AGGAAGCCAG 
GTGCTTCAGG TGCCAGCTGT AAATTTGCAC CCTATCAGGA AGAAGGGCCA AGGAAAAGAC 
CCTGCCACCC TGAGTACAGG TTTACCTCAG AAAATTTTAG GAACAGCTGA AGAAGTAAGT 
GGTGGTAAGA AACACACAGA AGACACCGTT TCTGTGGCAT CATCCTTACA TTCCAGCCCT 
CCTGTGTCTC CTCAAGGTTC CCCCCGCAAA GGTTATACAC TTATACCATC AGCTAAATCT 
GACAACTTGT CTGACTCCAG CCACAGTGAG ATTTCTTCAC GGTCCAGCAT TGTGAGCAAT 
TGTTCTGTTG ACTCCATGTC TACAGCTCTA CAGGATGAAC GGTGTTCCTC CCAGGCTGTG 
GCAGTCCCTG AATCCACTGG AGCATTGGAA AAGACAGAGC ACTCCTCAGG GATAGGAGAC 
CATAGTCAGC TTGGCCCTGG GTGGACACTC TCAAAGCCAT CTCTAATCAA CAAGAGTTTA 
GCTGTCTCAT CTTCTGTGAG CAATGAAGAG ATTTCTCATG AGCACATCAT TATAGAAACA 
GCTGATAGTG GTCGTGGAAG TTGGACTTCC TGCTCAAGCA GTTCCCATGA CAACTTCCAA 
AGTCTTCCAA ACCCCAAAGG CTGGGAGTTT TTGAACTCTT ACCGACCTAC CCATTTGGAT 
GGTCCCATAG CTGAAGTTGA ACCCACTGAC TGTGAGCACT ACAGCTGTCC CAAAGGCTGC 
TCTCGAACTT GTGGTCAGTG TAAAGGAAGC CTAGAGACTA ATCAGATGAG ACAGAGTTGG 
GCTTCCTCTA GTTCTCTGTC TGACACATAC GAACCAAACT ATGGGACAGT TAAACGGAGA 
GTGCTGGAGA ACACCCCAGC TGAATCATCT GAAGGCTTGG ACCACAAAGA GGGCATCGAC 
CCAGTCTATA AAACTGTTAC TTCAAGTACA GAAAAGGGCT TGATTGTGTA CTGTGTCACC 
TCACCCAAGA AGGAGGATAG GTATAGGGAG CCACCTCCCA CTCCTCCAGG ATACATGGGG 
ATTTCTTTAG CGGACCTAAA GGAAGGACCC CACCTCCACC TAAAACCTCC AGAGTATAGT 
GTGGCGGTGC AGAGGTCAAA GATGATGCAT AACAGCCTCT CTAGACTGCC ACCAGCTTCT 
CTCAGTAGCA GCCCTGTGGC CTGTGTTCCA TCGAAGATTG TAATTCAGCC TCAGAGGCAT 
AAACTAGATG TGACTGATGG AGATAGCGAA GCAGATGAAA ATGAACAGGT TTCAGCAGTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_005209077.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209134.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

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CloneID	OBa21251
Clone ID Related Accession (Same CDS sequence)	XM_005209077.3 , XM_015464188.1
Accession Version	XM_005209077.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209077.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209134.1
CDS	224..4759
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_005209077.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209077.3

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_015464188.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319674.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa21251
Clone ID Related Accession (Same CDS sequence)	XM_005209077.3 , XM_015464188.1
Accession Version	XM_015464188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319674.1
CDS	224..4759
Translation	MNSPVDPGARQALKKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLMKDSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENSKDNEDNILQREIPARQSRRRFRKINCKGERQTITDDVDINSYLSLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIENEQVSAV

Target ORF information:

RefSeq Version	XM_015464188.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464188.1

ORF Insert Sequence:

ATGAACTCAC CTGTGGATCC TGGCGCTAGG CAGGCGCTGA AGAAGAAGCC GCCAGAGCGG 
ACGCCCGAGG ACTTAAATAC TATTTATTCT TATCTTCATG GAATGGAAAT ATTATCAAAT 
CTCAGGGAAC ATCAGCTTCG ATTGATGTCT GCAAGAGCAC GCTATGAGAG ATACAGTGGC 
AATCAAGTTC TTTTTTGTTC AGAAACTATT GCCAGATGTT GGTATATCCT GCTTTCTGGA 
TCTGTGCTTA TGAAAGACTC CATGGTTTTG CCTCCTTGCA GTTTTGGTAA GCAGTTTGGA 
GGAAAAAGAG GATGTGATTG TCTTGTATTA GAGCCTTCAG AAATGATTGT GGTAGAGAAT 
TCCAAAGATA ATGAAGATAA TATTCTACAA AGAGAAATTC CTGCCAGACA GTCTCGAAGA 
AGATTTCGGA AAATTAATTG TAAAGGAGAA CGCCAGACTA TTACTGATGA TGTGGATATT 
AACAGCTATC TTTCTCTTCC AGCCGATCTT ACCAAGATGC ACATTGCAGA CAACCCTCAT 
CCACAGGTGA CTCACGTGTC TTCTAGTCAG TCTGGCTGTA GCATCGCCAG TGACTCTGGA 
AGCAGCAGTT TATCTGATAT CTATCAGGCT ACCGAAAGTG AGGTAGGAGA TGTCGATTTA 
ACACGTCTTC CAGAAGGACC TGTTGATTCT GAAGATGAAG AAGAGGAAGA TGAAGACATT 
GATCGAACAG ATCCTCTGCA AGGGCGGGAT CTTGTCCGAG AATGTCTTGA AAAAGAACCT 
GCAGATAAAA CTGATGATGA CATTGAACAA TTGCTGGAGT TTATGCACCA GCTTCCCGCT 
TTTGCAAATA TGACCATGTC TGTAAGGAGA GAACTCTGCT CAGTGATGAT TTTTGAAGTG 
GTAGAGCAGG CTGGAGCTGT CATTCTTGAA GATGGGCAAG AGCTTGATTC ATGGTATGTT 
ATTTTAAATG GCACTGTGGA AATCAGTCAT CCAGATGGAA AAGTTGACAA TCTCTTTATG 
GGAAATAGTT TTGGAATCAC ACCCACTCTG GATAAACAGT ACATGCATGG AGTTGTTAGG 
ACTAAAGTAG ATGACTGTCA GTTTGTCTGC ATAGCCCAGC AGGATTATTG GAGAATTTTA 
AACCATGTTG AAAAAAATAC CCATAAAGTT GAGGAAGAGG GAGAAATTGT TATGGTACAT 
GAGCATCGTG AACTGGATCG AAGTGGAACC AGGAAAGGAC ACATTGTAAT TAAGGGAACA 
CCTGAGCGTC TCATCATGCA TTTAATAGAA GAGCATTCCA TTGTGGACCC AACTTATATA 
GAAGATTTCC TATTAACTTA CAGAACATTT CTTGCAAGTC CTTTGGATGT TGGGACCAAA 
CTGTTGGAAT GGTTTAAAAT TGACAGCTTA AGGGATAAGG TAACACGGAT TGTATTATTA 
TGGGTAAATA ATCATTTCAA TGATTTTGAA GGTGATCCTG CTATGACTCA ATTTCTAGAG 
GAATTTGAAA AAAATCTGGA AGATACAAAA ATGAATGGTC ATCTCCGGTT ATTGAATATT 
GCGTGTGCTG CAAAGGCTAA GTGGAGACAG GTTGTGCTGC AGAAAGCTTC CCGGGAGTCA 
CCTCTGCATT TCAGCCTAAG TGGAGGAAGT GAGAAGGGAT TTGGTATTTT TGTTGAAAGC 
GTAGAACCTA GTAGCAAAGC TGCTGATGCA GGACTGAAAC GTGGTGATCA AGTAATGGAA 
GTAAATGGAC AAAATTTTGA GAATATAACG TTTGTGAAGG CCCTTGAAAT TTTGAGGAAT 
AATACTCATC TTGCACTTAC TGTAAAGACC AACATTTTTG TGTTCAAAGA GTTACTTAGT 
AGGACTGAAC AAGAGAAATC TGGTGTTCCT CATATTCCCA AAATTGCTGA AAAAAAAAGT 
AATCGCCACT CAATCCAGGA TGTACCAGGA GATCTTGAAC AGACATCACA GGAGAAAGGA 
AATAAGAAAA TAAAAGCAAA TACCGTTTCA GGTGGAAGAA ACAAAATCAG GAAAATTTTG 
GATAAAACAC GATTTAGTAT CTTGCCTCCA AAACTTTTTA GTGATGGAGG CCTGAGCCAG 
TCACAGGATG ACAGCATTGT GGGAACGAGA CACTGCAGGC ACAGTCTGGC CATCATGCCT 
ATCCCTGGAA CACTCTCGTC CAGCAGCCCC GACCTCCTCC AGCCTACCAC CAGCATGTTG 
GATTTTTCTA ATCCCTCAGA TATTCCTGAT CAAGTTATAA GAGTTTTCAA AGCAGATCAG 
CAAAGTTGCT ACATTATCAT CAGTAAAGAC ACTACAGCTA AGGAAGTTGT CTGTCAAGCT 
ATTCATGAAT TTGGTTTGAC TGGTGCATCT GACACATACT CTCTCTGTGA AGTTTCTGTT 
ACTCCTGAGG GTGTTATAAA ACAGAGAAGA CTTCCAGACC AATTCTCCAA ATTAGCTGAT 
AGAATTCAGC TCAATGGAAG GTATTATTTG AAAAATAACA TGGAAACAGA AACCCTATGT 
TCAGATGAAG ATGCTCAAGA ACTAGTTAAG GAAAGCCAGC TGTCCATGCT ACAACTAAGT 
ACTATTGAGG TGGCCACTCA GCTGTCAATG AGGGACTTTG ATTTATTTCG TAATATTGAG 
CCTACTGAAT ATATTGATGA CCTTTTTAAG TTAGAGTCCA AAACAGGAAA TACTCACTTG 
AAAGAATTTG AAGACATTGT AAACCAAGAG ACATTCTGGG TTGCATCAGA GATTTTAACT 
GAATCAAATC AGCTCAAACG AATGAAGATT ATTAAACATT TTATTAAAAT TGCACTTCAT 
TGTCGAGAAT GTAAGAACTT CAATTCCATG TTTGCAATAA TAAGTGGTTT GAACTTGGCA 
TCTGTAGCAC GACTCAGAGG TACTTGGGAA AAGTTACCAA GCAAGTATGA TAAACACTTC 
CAAGATCTGC AAGACCTTTT TGATCCATCC AGAAACATGG CAAAATACAG AAATATTCTT 
AGTAGTCAAA GCATGCAGCC TCCAATTATT CCACTCTTTC CTGTTGTCAA GAAAGATATG 
ACATTTCTAC ATGAAGGAAA TGATTCCAAA GTTGATGGTT TGGTAAATTT CGAGAAACTC 
AGAATGATTG CCAAGGAAAT TCGTCAGGTT GTTCGAATGA CTTCTGCTAA CATGGATCCA 
GCTATGATGT TCCGACAGAG GAAGAAGAGG TGGCGGAGTC TGGGGTCACT GAGTCAAGGC 
AGCACAAATT CAAACATGCT GGATGTTCAG GGAGGTGCTC ACAAAAAAAG GGCACGCCGT 
AGCTCTCTGC TAAATGCCAA GAAGCTATAT GAAGATGCCC AAATGGCAAG AAAAGTGAAG 
CAGTATCTTT CCAGTCTGGA TGTCGAGACA GATGAAGAGA AGTTCCAGAT GATGTCATTG 
CAATGGGAGC CTGCATATGG TACCTTGACC AAGAATTTAA ATGAGAAAAA ATCAATGAAG 
TCATCAGAAA TGTCTCCGGT GCCACTGAGG TCAGCTGGCC AAACAACTAA AGCTCATTTG 
CATCAGCCTC ACAGAGGAAG CCAGGTGCTT CAGGTGCCAG CTGTAAATTT GCACCCTATC 
AGGAAGAAGG GCCAAGGAAA AGACCCTGCC ACCCTGAGTA CAGGTTTACC TCAGAAAATT 
TTAGGAACAG CTGAAGAAGT AAGTGGTGGT AAGAAACACA CAGAAGACAC CGTTTCTGTG 
GCATCATCCT TACATTCCAG CCCTCCTGTG TCTCCTCAAG GTTCCCCCCG CAAAGGTTAT 
ACACTTATAC CATCAGCTAA ATCTGACAAC TTGTCTGACT CCAGCCACAG TGAGATTTCT 
TCACGGTCCA GCATTGTGAG CAATTGTTCT GTTGACTCCA TGTCTACAGC TCTACAGGAT 
GAACGGTGTT CCTCCCAGGC TGTGGCAGTC CCTGAATCCA CTGGAGCATT GGAAAAGACA 
GAGCACTCCT CAGGGATAGG AGACCATAGT CAGCTTGGCC CTGGGTGGAC ACTCTCAAAG 
CCATCTCTAA TCAACAAGAG TTTAGCTGTC TCATCTTCTG TGAGCAATGA AGAGATTTCT 
CATGAGCACA TCATTATAGA AACAGCTGAT AGTGGTCGTG GAAGTTGGAC TTCCTGCTCA 
AGCAGTTCCC ATGACAACTT CCAAAGTCTT CCAAACCCCA AAGGCTGGGA GTTTTTGAAC 
TCTTACCGAC CTACCCATTT GGATGGTCCC ATAGCTGAAG TTGAACCCAC TGACTGTGAG 
CACTACAGCT GTCCCAAAGG CTGCTCTCGA ACTTGTGGTC AGTGTAAAGG AAGCCTAGAG 
ACTAATCAGA TGAGACAGAG TTGGGCTTCC TCTAGTTCTC TGTCTGACAC ATACGAACCA 
AACTATGGGA CAGTTAAACG GAGAGTGCTG GAGAACACCC CAGCTGAATC ATCTGAAGGC 
TTGGACCACA AAGAGGGCAT CGACCCAGTC TATAAAACTG TTACTTCAAG TACAGAAAAG 
GGCTTGATTG AAAATGAACA GGTTTCAGCA GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_015464189.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319675.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa21253
Clone ID Related Accession (Same CDS sequence)	XM_015464189.1 , XM_005209079.3
Accession Version	XM_015464189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319675.1
CDS	861..5246
Translation	MAFLVRCYANCLQPWASKLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_015464189.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015464189.1

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF6 ( XM_005209079.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209136.1 Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

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CloneID	OBa21253
Clone ID Related Accession (Same CDS sequence)	XM_015464189.1 , XM_005209079.3
Accession Version	XM_005209079.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rap guanine nucleotide exchange factor 6 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209079.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209136.1
CDS	861..5246
Translation	MAFLVRCYANCLQPWASKLPADLTKMHIADNPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDVDLTRLPEGPVDSEDEEEEDEDIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMHQLPAFANMTMSVRRELCSVMIFEVVEQAGAVILEDGQELDSWYVILNGTVEISHPDGKVDNLFMGNSFGITPTLDKQYMHGVVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEEEGEIVMVHEHRELDRSGTRKGHIVIKGTPERLIMHLIEEHSIVDPTYIEDFLLTYRTFLASPLDVGTKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTQFLEEFEKNLEDTKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLHFSLSGGSEKGFGIFVESVEPSSKAADAGLKRGDQVMEVNGQNFENITFVKALEILRNNTHLALTVKTNIFVFKELLSRTEQEKSGVPHIPKIAEKKSNRHSIQDVPGDLEQTSQEKGNKKIKANTVSGGRNKIRKILDKTRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSMLDFSNPSDIPDQVIRVFKADQQSCYIIISKDTTAKEVVCQAIHEFGLTGASDTYSLCEVSVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSMLQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLESKTGNTHLKEFEDIVNQETFWVASEILTESNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKLPSKYDKHFQDLQDLFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSKVDGLVNFEKLRMIAKEIRQVVRMTSANMDPAMMFRQRKKRWRSLGSLSQGSTNSNMLDVQGGAHKKRARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLNEKKSMKSSEMSPVPLRSAGQTTKAHLHQPHRGSQVLQVPAVNLHPIRKKGQGKDPATLSTGLPQKILGTAEEVSGGKKHTEDTVSVASSLHSSPPVSPQGSPRKGYTLIPSAKSDNLSDSSHSEISSRSSIVSNCSVDSMSTALQDERCSSQAVAVPESTGALEKTEHSSGIGDHSQLGPGWTLSKPSLINKSLAVSSSVSNEEISHEHIIIETADSGRGSWTSCSSSSHDNFQSLPNPKGWEFLNSYRPTHLDGPIAEVEPTDCEHYSCPKGCSRTCGQCKGSLETNQMRQSWASSSSLSDTYEPNYGTVKRRVLENTPAESSEGLDHKEGIDPVYKTVTSSTEKGLIVYCVTSPKKEDRYREPPPTPPGYMGISLADLKEGPHLHLKPPEYSVAVQRSKMMHNSLSRLPPASLSSSPVACVPSKIVIQPQRHKLDVTDGDSEADENEQVSAV

Target ORF information:

RefSeq Version	XM_005209079.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rap guanine nucleotide exchange factor 6 (RAPGEF6), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209079.3

ORF Insert Sequence:

ATGGCTTTTC TTGTTCGCTG TTATGCCAAT TGTCTTCAGC CATGGGCCTC CAAACTTCCA 
GCCGATCTTA CCAAGATGCA CATTGCAGAC AACCCTCATC CACAGGTGAC TCACGTGTCT 
TCTAGTCAGT CTGGCTGTAG CATCGCCAGT GACTCTGGAA GCAGCAGTTT ATCTGATATC 
TATCAGGCTA CCGAAAGTGA GGTAGGAGAT GTCGATTTAA CACGTCTTCC AGAAGGACCT 
GTTGATTCTG AAGATGAAGA AGAGGAAGAT GAAGACATTG ATCGAACAGA TCCTCTGCAA 
GGGCGGGATC TTGTCCGAGA ATGTCTTGAA AAAGAACCTG CAGATAAAAC TGATGATGAC 
ATTGAACAAT TGCTGGAGTT TATGCACCAG CTTCCCGCTT TTGCAAATAT GACCATGTCT 
GTAAGGAGAG AACTCTGCTC AGTGATGATT TTTGAAGTGG TAGAGCAGGC TGGAGCTGTC 
ATTCTTGAAG ATGGGCAAGA GCTTGATTCA TGGTATGTTA TTTTAAATGG CACTGTGGAA 
ATCAGTCATC CAGATGGAAA AGTTGACAAT CTCTTTATGG GAAATAGTTT TGGAATCACA 
CCCACTCTGG ATAAACAGTA CATGCATGGA GTTGTTAGGA CTAAAGTAGA TGACTGTCAG 
TTTGTCTGCA TAGCCCAGCA GGATTATTGG AGAATTTTAA ACCATGTTGA AAAAAATACC 
CATAAAGTTG AGGAAGAGGG AGAAATTGTT ATGGTACATG AGCATCGTGA ACTGGATCGA 
AGTGGAACCA GGAAAGGACA CATTGTAATT AAGGGAACAC CTGAGCGTCT CATCATGCAT 
TTAATAGAAG AGCATTCCAT TGTGGACCCA ACTTATATAG AAGATTTCCT ATTAACTTAC 
AGAACATTTC TTGCAAGTCC TTTGGATGTT GGGACCAAAC TGTTGGAATG GTTTAAAATT 
GACAGCTTAA GGGATAAGGT AACACGGATT GTATTATTAT GGGTAAATAA TCATTTCAAT 
GATTTTGAAG GTGATCCTGC TATGACTCAA TTTCTAGAGG AATTTGAAAA AAATCTGGAA 
GATACAAAAA TGAATGGTCA TCTCCGGTTA TTGAATATTG CGTGTGCTGC AAAGGCTAAG 
TGGAGACAGG TTGTGCTGCA GAAAGCTTCC CGGGAGTCAC CTCTGCATTT CAGCCTAAGT 
GGAGGAAGTG AGAAGGGATT TGGTATTTTT GTTGAAAGCG TAGAACCTAG TAGCAAAGCT 
GCTGATGCAG GACTGAAACG TGGTGATCAA GTAATGGAAG TAAATGGACA AAATTTTGAG 
AATATAACGT TTGTGAAGGC CCTTGAAATT TTGAGGAATA ATACTCATCT TGCACTTACT 
GTAAAGACCA ACATTTTTGT GTTCAAAGAG TTACTTAGTA GGACTGAACA AGAGAAATCT 
GGTGTTCCTC ATATTCCCAA AATTGCTGAA AAAAAAAGTA ATCGCCACTC AATCCAGGAT 
GTACCAGGAG ATCTTGAACA GACATCACAG GAGAAAGGAA ATAAGAAAAT AAAAGCAAAT 
ACCGTTTCAG GTGGAAGAAA CAAAATCAGG AAAATTTTGG ATAAAACACG ATTTAGTATC 
TTGCCTCCAA AACTTTTTAG TGATGGAGGC CTGAGCCAGT CACAGGATGA CAGCATTGTG 
GGAACGAGAC ACTGCAGGCA CAGTCTGGCC ATCATGCCTA TCCCTGGAAC ACTCTCGTCC 
AGCAGCCCCG ACCTCCTCCA GCCTACCACC AGCATGTTGG ATTTTTCTAA TCCCTCAGAT 
ATTCCTGATC AAGTTATAAG AGTTTTCAAA GCAGATCAGC AAAGTTGCTA CATTATCATC 
AGTAAAGACA CTACAGCTAA GGAAGTTGTC TGTCAAGCTA TTCATGAATT TGGTTTGACT 
GGTGCATCTG ACACATACTC TCTCTGTGAA GTTTCTGTTA CTCCTGAGGG TGTTATAAAA 
CAGAGAAGAC TTCCAGACCA ATTCTCCAAA TTAGCTGATA GAATTCAGCT CAATGGAAGG 
TATTATTTGA AAAATAACAT GGAAACAGAA ACCCTATGTT CAGATGAAGA TGCTCAAGAA 
CTAGTTAAGG AAAGCCAGCT GTCCATGCTA CAACTAAGTA CTATTGAGGT GGCCACTCAG 
CTGTCAATGA GGGACTTTGA TTTATTTCGT AATATTGAGC CTACTGAATA TATTGATGAC 
CTTTTTAAGT TAGAGTCCAA AACAGGAAAT ACTCACTTGA AAGAATTTGA AGACATTGTA 
AACCAAGAGA CATTCTGGGT TGCATCAGAG ATTTTAACTG AATCAAATCA GCTCAAACGA 
ATGAAGATTA TTAAACATTT TATTAAAATT GCACTTCATT GTCGAGAATG TAAGAACTTC 
AATTCCATGT TTGCAATAAT AAGTGGTTTG AACTTGGCAT CTGTAGCACG ACTCAGAGGT 
ACTTGGGAAA AGTTACCAAG CAAGTATGAT AAACACTTCC AAGATCTGCA AGACCTTTTT 
GATCCATCCA GAAACATGGC AAAATACAGA AATATTCTTA GTAGTCAAAG CATGCAGCCT 
CCAATTATTC CACTCTTTCC TGTTGTCAAG AAAGATATGA CATTTCTACA TGAAGGAAAT 
GATTCCAAAG TTGATGGTTT GGTAAATTTC GAGAAACTCA GAATGATTGC CAAGGAAATT 
CGTCAGGTTG TTCGAATGAC TTCTGCTAAC ATGGATCCAG CTATGATGTT CCGACAGAGG 
AAGAAGAGGT GGCGGAGTCT GGGGTCACTG AGTCAAGGCA GCACAAATTC AAACATGCTG 
GATGTTCAGG GAGGTGCTCA CAAAAAAAGG GCACGCCGTA GCTCTCTGCT AAATGCCAAG 
AAGCTATATG AAGATGCCCA AATGGCAAGA AAAGTGAAGC AGTATCTTTC CAGTCTGGAT 
GTCGAGACAG ATGAAGAGAA GTTCCAGATG ATGTCATTGC AATGGGAGCC TGCATATGGT 
ACCTTGACCA AGAATTTAAA TGAGAAAAAA TCAATGAAGT CATCAGAAAT GTCTCCGGTG 
CCACTGAGGT CAGCTGGCCA AACAACTAAA GCTCATTTGC ATCAGCCTCA CAGAGGAAGC 
CAGGTGCTTC AGGTGCCAGC TGTAAATTTG CACCCTATCA GGAAGAAGGG CCAAGGAAAA 
GACCCTGCCA CCCTGAGTAC AGGTTTACCT CAGAAAATTT TAGGAACAGC TGAAGAAGTA 
AGTGGTGGTA AGAAACACAC AGAAGACACC GTTTCTGTGG CATCATCCTT ACATTCCAGC 
CCTCCTGTGT CTCCTCAAGG TTCCCCCCGC AAAGGTTATA CACTTATACC ATCAGCTAAA 
TCTGACAACT TGTCTGACTC CAGCCACAGT GAGATTTCTT CACGGTCCAG CATTGTGAGC 
AATTGTTCTG TTGACTCCAT GTCTACAGCT CTACAGGATG AACGGTGTTC CTCCCAGGCT 
GTGGCAGTCC CTGAATCCAC TGGAGCATTG GAAAAGACAG AGCACTCCTC AGGGATAGGA 
GACCATAGTC AGCTTGGCCC TGGGTGGACA CTCTCAAAGC CATCTCTAAT CAACAAGAGT 
TTAGCTGTCT CATCTTCTGT GAGCAATGAA GAGATTTCTC ATGAGCACAT CATTATAGAA 
ACAGCTGATA GTGGTCGTGG AAGTTGGACT TCCTGCTCAA GCAGTTCCCA TGACAACTTC 
CAAAGTCTTC CAAACCCCAA AGGCTGGGAG TTTTTGAACT CTTACCGACC TACCCATTTG 
GATGGTCCCA TAGCTGAAGT TGAACCCACT GACTGTGAGC ACTACAGCTG TCCCAAAGGC 
TGCTCTCGAA CTTGTGGTCA GTGTAAAGGA AGCCTAGAGA CTAATCAGAT GAGACAGAGT 
TGGGCTTCCT CTAGTTCTCT GTCTGACACA TACGAACCAA ACTATGGGAC AGTTAAACGG 
AGAGTGCTGG AGAACACCCC AGCTGAATCA TCTGAAGGCT TGGACCACAA AGAGGGCATC 
GACCCAGTCT ATAAAACTGT TACTTCAAGT ACAGAAAAGG GCTTGATTGT GTACTGTGTC 
ACCTCACCCA AGAAGGAGGA TAGGTATAGG GAGCCACCTC CCACTCCTCC AGGATACATG 
GGGATTTCTT TAGCGGACCT AAAGGAAGGA CCCCACCTCC ACCTAAAACC TCCAGAGTAT 
AGTGTGGCGG TGCAGAGGTC AAAGATGATG CATAACAGCC TCTCTAGACT GCCACCAGCT 
TCTCTCAGTA GCAGCCCTGT GGCCTGTGTT CCATCGAAGA TTGTAATTCA GCCTCAGAGG 
CATAAACTAG ATGTGACTGA TGGAGATAGC GAAGCAGATG AAAATGAACA GGTTTCAGCA 
GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RAPGEF6
Entrez Gene ID	789597
Full Name	Rap guanine nucleotide exchange factor 6
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Bos taurus (cattle)	RAPGEF6	XP_003586323.1
Homo sapiens (human)	RAPGEF6	NP_001157858.1
Canis lupus familiaris (dog)	RAPGEF6	XP_003431670.1
Mus musculus (house mouse)	Rapgef6	NP_780467.2
Gallus gallus (chicken)	RAPGEF6	XP_004945062.1
Pan troglodytes (chimpanzee)	RAPGEF6	XP_003339150.1
	RAPGEF6	XP_001101236.2
Rattus norvegicus (Norway rat)	Rapgef6	NP_001100473.1
Danio rerio (zebrafish)	rapgef6	XP_002666140.3
Caenorhabditis elegans (roundworm)	pxf-1	NP_001023389.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RAPGEF6 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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