TOP2A cDNA ORF clone, Bos taurus(cattle)

The following TOP2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa93954 XM_015458846.2
Latest version!
Bos taurus DNA topoisomerase II alpha (TOP2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa93954 XM_015468142.1
Latest version!
Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa10222 XM_005195726.3
Latest version!
Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa10222 XM_005220781.4
Latest version!
Bos taurus DNA topoisomerase II alpha (TOP2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa58213 XM_010824139.1
Latest version!
Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa58213 XM_010816273.1
Latest version!
Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa10222 XM_005220781.3 Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa93954 XM_015458846.1 Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa93954
    Clone ID Related Accession (Same CDS sequence) XM_015458846.2 , XM_015468142.1 , XM_015458846.1
    Accession Version XM_015458846.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GCACCAAAAA AAGGGCTGCA CCAAAAAAAA CCAAAAAGGA TCCAGACTTG
    GATTCTGATG TCTCTAAAAA GCCTAATGCC CCCAAAACCA AGACTCGCCG CAAAAGGAAG
    GCGTCCTCCT CTGATGATTC CGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA
    AGCAAGAAAC CCAAGGAGGA AAGTGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT
    TCTCGGGCAA AATCTGTAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA
    GATGATTTCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314332.1
    CDS204..4778
    Translation

    Target ORF information:

    RefSeq Version XM_015458846.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II alpha (TOP2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458846.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GCACCAAAAA AAGGGCTGCA CCAAAAAAAA CCAAAAAGGA TCCAGACTTG
    GATTCTGATG TCTCTAAAAA GCCTAATGCC CCCAAAACCA AGACTCGCCG CAAAAGGAAG
    GCGTCCTCCT CTGATGATTC CGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA
    AGCAAGAAAC CCAAGGAGGA AAGTGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT
    TCTCGGGCAA AATCTGTAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA
    GATGATTTCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93954
    Clone ID Related Accession (Same CDS sequence) XM_015458846.2 , XM_015468142.1 , XM_015458846.1
    Accession Version XM_015468142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GCACCAAAAA AAGGGCTGCA CCAAAAAAAA CCAAAAAGGA TCCAGACTTG
    GATTCTGATG TCTCTAAAAA GCCTAATGCC CCCAAAACCA AGACTCGCCG CAAAAGGAAG
    GCGTCCTCCT CTGATGATTC CGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA
    AGCAAGAAAC CCAAGGAGGA AAGTGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT
    TCTCGGGCAA AATCTGTAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA
    GATGATTTCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323628.1
    CDS140..4714
    Translation

    Target ORF information:

    RefSeq Version XM_015468142.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GCACCAAAAA AAGGGCTGCA CCAAAAAAAA CCAAAAAGGA TCCAGACTTG
    GATTCTGATG TCTCTAAAAA GCCTAATGCC CCCAAAACCA AGACTCGCCG CAAAAGGAAG
    GCGTCCTCCT CTGATGATTC CGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA
    AGCAAGAAAC CCAAGGAGGA AAGTGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT
    TCTCGGGCAA AATCTGTAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA
    GATGATTTCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93954
    Clone ID Related Accession (Same CDS sequence) XM_015458846.2 , XM_015468142.1 , XM_015458846.1
    Accession Version XM_015458846.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GCACCAAAAA AAGGGCTGCA CCAAAAAAAA CCAAAAAGGA TCCAGACTTG
    GATTCTGATG TCTCTAAAAA GCCTAATGCC CCCAAAACCA AGACTCGCCG CAAAAGGAAG
    GCGTCCTCCT CTGATGATTC CGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA
    AGCAAGAAAC CCAAGGAGGA AAGTGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT
    TCTCGGGCAA AATCTGTAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA
    GATGATTTCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314332.1
    CDS140..4714
    Translation

    Target ORF information:

    RefSeq Version XM_015458846.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458846.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GCACCAAAAA AAGGGCTGCA CCAAAAAAAA CCAAAAAGGA TCCAGACTTG
    GATTCTGATG TCTCTAAAAA GCCTAATGCC CCCAAAACCA AGACTCGCCG CAAAAGGAAG
    GCGTCCTCCT CTGATGATTC CGACTCTAAT TTTGAGAAAC TGATCTCCAA AGCAGTCACA
    AGCAAGAAAC CCAAGGAGGA AAGTGATGAT TTCCATCTGG ACTTGGACTC AGCTGTGGGT
    TCTCGGGCAA AATCTGTAAG AACAAAGAAA CCTATAAAGT ACCTTGAAGA ATCAGATGAA
    GATGATTTCT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10222
    Clone ID Related Accession (Same CDS sequence) XM_005220781.3 , XM_005220781.4 , XM_005195726.3
    Accession Version XM_005220781.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220781.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GTCAGTCTTC CACCTCCACT ACAGGCACCA AAAAAAGGGC TGCACCAAAA
    AAAACCAAAA AGGATCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCCAAA
    ACCAAGACTC GCCGCAAAAG GAAGGCGTCC TCCTCTGATG ATTCCGACTC TAATTTTGAG
    AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGTGA TGATTTCCAT
    CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG TAAGAACAAA GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220838.1
    CDS140..4738
    Translation

    Target ORF information:

    RefSeq Version XM_005220781.3
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220781.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GTCAGTCTTC CACCTCCACT ACAGGCACCA AAAAAAGGGC TGCACCAAAA
    AAAACCAAAA AGGATCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCCAAA
    ACCAAGACTC GCCGCAAAAG GAAGGCGTCC TCCTCTGATG ATTCCGACTC TAATTTTGAG
    AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGTGA TGATTTCCAT
    CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG TAAGAACAAA GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10222
    Clone ID Related Accession (Same CDS sequence) XM_005220781.3 , XM_005220781.4 , XM_005195726.3
    Accession Version XM_005220781.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220781.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GTCAGTCTTC CACCTCCACT ACAGGCACCA AAAAAAGGGC TGCACCAAAA
    AAAACCAAAA AGGATCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCCAAA
    ACCAAGACTC GCCGCAAAAG GAAGGCGTCC TCCTCTGATG ATTCCGACTC TAATTTTGAG
    AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGTGA TGATTTCCAT
    CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG TAAGAACAAA GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220838.1
    CDS204..4802
    Translation

    Target ORF information:

    RefSeq Version XM_005220781.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II alpha (TOP2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220781.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GTCAGTCTTC CACCTCCACT ACAGGCACCA AAAAAAGGGC TGCACCAAAA
    AAAACCAAAA AGGATCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCCAAA
    ACCAAGACTC GCCGCAAAAG GAAGGCGTCC TCCTCTGATG ATTCCGACTC TAATTTTGAG
    AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGTGA TGATTTCCAT
    CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG TAAGAACAAA GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10222
    Clone ID Related Accession (Same CDS sequence) XM_005220781.3 , XM_005220781.4 , XM_005195726.3
    Accession Version XM_005195726.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195726.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GTCAGTCTTC CACCTCCACT ACAGGCACCA AAAAAAGGGC TGCACCAAAA
    AAAACCAAAA AGGATCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCCAAA
    ACCAAGACTC GCCGCAAAAG GAAGGCGTCC TCCTCTGATG ATTCCGACTC TAATTTTGAG
    AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGTGA TGATTTCCAT
    CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG TAAGAACAAA GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195783.1
    CDS140..4738
    Translation

    Target ORF information:

    RefSeq Version XM_005195726.3
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195726.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGTGT CGCCACTACA GCCTGTAAAT GAAAATATGC AAGTCAGCAA AACAAAGAAA 
    AATGAAGATG CTAAGAAAAG ATTGTCTATT GAAAGAATCT ATCAAAAGAA AACACAACTG
    GAACATATCC TGCTCCGCCC AGACACCTAC ATTGGCTCTG TGGAATCAGT GACCCAGCAA
    ATGTGGGTTT ATGATGAAGA TATTGGCATT AACTATAGGG AAGTCACTTA TGTTCCTGGT
    TTGTACAAAA TCTTTGATGA GATTCTAGTC AATGCTGCAG ACAACAAACA AAGGGACCCA
    AAAATGTCTT GTATTAGAAT CACAATTGAC CCGGAAAACA ATTTAATTAG TATATGGAAT
    AATGGAAAAG GTATTCCTGT TGTCGAACAC AAAGTTGAGA AGATGTATGT CCCAGCTCTT
    ATCTTTGGAC AGCTCCTAAC TTCTAGCAAC TATGATGATG AAGAAAAGAA AGTAACAGGT
    GGTCGAAATG GCTATGGAGC CAAACTGTGT AACATATTCA GTACCAAATT TACTGTGGAA
    ACAGCAAGTA GAGAATACAA GAAAATGTTC AAACAGACAT GGATGGATAA CATGGGGAAA
    GCTGGTGAGA TGGAACTCAA GCCCTTTAGT GGAGAAGATT ATACATGTAT TACCTTCCAC
    CCTGATTTGT CCAAGTTTAA AATGCAAAGC CTAGACAAAG ATATTGTTGC ACTGATGGTT
    AGAAGAGCAT ATGATATTGC TGGATCCACT AAAGATGTCA AAGTCTATCT CAATGGAAAT
    AGGCTTCCAG TGAAAGGATT CCGTAGCTAT GTAGATCTGT ATCTGAAGGA TAAGGTAGAT
    GAAACTGGCA ATCCATTGAA AGTTATCCAT GAACAAGTAA ACCACAGGTG GGAAGTATGT
    TTAACAATGA GTGAAAAAGG CTTTCAGCAA ATTAGCTTTG TCAACAGCAT TGCTACTTCC
    AAGGGTGGCC GACATGTTGA TTATGTAGCT GATCAGATTG TGACTAAACT TGTTGATGTT
    GTGAAGAAAA AGAACAAGGG TGGTGTTGCA GTAAAAGCAC ATCAGGTAAA AAATCACATG
    TGGATTTTTG TGAATGCCTT AATTGAAAAC CCAACCTTCG ACTCCCAGAC AAAAGAGAAC
    ATGACTTTAC AAGTCAAGAG CTTTGGATCA ACATGCCAGC TGAGTGAAAA ATTCATCAAA
    GCTGCGATTG GCTGTGGTAT TGTAGAAAGT ATACTAAATT GGGTGAAATT TAAGGCCCAA
    ATCCAGTTAA ACAAGAAGTG TTCTGCTGTA AAACATAACA GAATCAAAGG AATTCCCAAA
    CTTGATGATG CCAATGATGC AGGAGGCCGA AACTCTACTG AGTGTACACT TATCCTGACA
    GAGGGAGACT CAGCTAAAAC TTTGGCTGTT TCAGGCCTTG GTGTGGTTGG GAGAGACAAG
    TATGGGGTTT TCCCTCTTAG AGGAAAAATC CTCAATGTTC GAGAAGCTTC TCATAAACAG
    ATAATGGAAA ATGCTGAAAT TAACAATATA ATTAAGATTG TGGGTCTTCA GTACAAGAAA
    AACTATGAAG ATGAAGATTC ACTGAAGACT CTACGTTATG GAAAGATAAT GATTATGACA
    GATCAGGACC AAGACGGTTC CCATATCAAA GGCTTGCTGA TTAATTTTAT CCATCACAAC
    TGGCCCTCTC TTCTGCGACA TCGTTTTCTG GAGGAATTTA TCACTCCCAT TGTAAAGGTG
    TCTAAAAACA AACAAGAAAT GGCATTCTAT AGCCTTCCTG AGTTTGAAGA ATGGAAGAGT
    TCTACACCAA ATCATAAAAA ATGGAAAGTC AAATATTACA AAGGTTTGGG TACCAGCACA
    TCAAAGGAAG CTAAAGAATA CTTTGCAGAT ATGAAAAGAC ATCGTATCCA ATTTAAATAT
    TCCGGTCCCG AAGATGATGC TGCAATCAGC CTGGCCTTTA GCAAAAAGCA GACAGATGAT
    CGAAAGGAAT GGTTAACTCA TTTCATGGAA GATAGAAGAC AACGAAAGTT ACTTGGCCTT
    CCTGATGATT ATTTGTATGG GCAAGCTACC ACATATCTGA CATACAATGA CTTCATAAAC
    AAGGAACTTA TCCTGTTTTC AAATTCTGAT AATGAAAGGT CTATCCCATC TATGGTGGAT
    GGTTTGAAAC CAGGTCAGAG AAAGGTTTTG TTTACATGTT TCAAACGGAA TGACAAGCGA
    GAGGTGAAGG TTGCCCAGTT AGCTGGGTCA GTAGCTGAGA TGTCCTCTTA TCATCATGGT
    GAGATGTCAC TAATGATGAC CATTATCAAT TTGGCTCAGA ACTTTGTGGG CAGCAATAAT
    CTGAACCTTT TGCAGCCCAT TGGTCAGTTT GGTACCAGGT TGCATGGTGG CAAGGACTCT
    GCTAGTCCTC GATACATCTT CACAATGCTC AGCCCTTTGG CTCGGTTGTT ATTTCCAGCA
    AAAGATGACC ATACATTAAA GTTTTTATAT GATGACAACC AGCGTGTTGA GCCTGAATGG
    TATATTCCTA TTATACCCAT GGTGTTGATA AATGGCGCTG AAGGAATTGG TACTGGGTGG
    TCCTGCAAAA TCCCCAACTT TGATGTTCGT GAAGTTGTGA ATAACATCAG ACGTTTGATG
    GATGGAGAAG AACCTTTGCC AATGCTTCCA AGTTACAAGA ACTTCAAGGG TACCGTTGAG
    GAGCTGGCTC CAAATCAATA TGTGATTAGT GGTGAAGTGG CTATTCTTAA TTCTACAACC
    ATTGAAATCT CAGAGCTTCC CATCAGAACG TGGACCCAGA CGTATAAGGA ACAGGTTCTT
    GAGCCCATGT TGAATGGCAC TGAGAAGACA CCGCCTCTCA TAACAGATTA TAGGGAATAC
    CACACAGACA CCACTGTGAA GTTTGTTGTG AAGATGACTG AAGAAAAGCT GGCGGAGGCA
    GAGAGAGCTG GACTACACAA AGTCTTCAAA CTCCAAACTA GTCTCACATG CAACTCTATG
    GTCCTCTTTG ACCATGTAGG CTGTTTAAAG AAATATGACA CAGTTTTGGA TATTCTAAGA
    GACTTCTTTG AACTCAGGCT TAAATATTAT GGATTAAGAA AAGAATGGCT TCTAGGAATG
    CTTGGTGCTG AATCTGCTAA ACTGAATAAT CAGGCTCGCT TTATCTTAGA GAAAATAGAT
    GGCAAAATAA TCATTGAAAA TAAGCCTAAG AAAGAGTTAA TTAAAGTTCT GATTCAGAGA
    GGATATGATT CGGATCCTGT GAAGGCTTGG AAAGAAGCCC AGCAAAAGGT TCCGGATGAA
    GAAGAAAATG AAGAAAGTGA CAGTGAAAAG GAAACTGAAA AGAGTGACTC TGCAACAGAT
    TCTGGACCTA CCTTCAACTA CCTTCTTGAC ATGCCTCTTT GGTATCTAAC CAAGGAAAAG
    AAAGATGAAC TGTGCAAACT AAGAAATGAA AAAGAGCAAG AGCTAGAAAC ATTGAAGAGA
    AAGAGTCCAT CAGATTTGTG GAAAGAAGAC TTGGCTGCTT TTGTTGAAGA GCTGGAGGTT
    GTTGAAGCAA AGGAGAAACA AGATGAACAA ATAGGACTTC CTGGGAAAGG TGGGAAAGCA
    AAGGGGAAAA AAACACAAAT GGTAGAAATT TTGCCTTCTC CAAGTGGAAA AAGAGTCATC
    CCCCGAGTGA CTGAAGAAAT GAAAGCAGAG GCAGAAAAGA AAATTAAAAA GAAAATTAAG
    AGTGAAAATA CTGAAGGAAG CCCTCAAGAG GATGGAATGG AAATGGAAAG CCAAAAACTG
    AGACTGGAAA AGAAACAGAA AAGAGAGCCA GTTACCAAGA CAAAGAAACA AACTACATTG
    CCATTTAAAC CAATCAAGAA AGCAAAGAAA AGAAACCCCT GGTCCGATTC AGAATCAGAT
    ATGAGCAGTA ACGAAAGTAA TTTTGATGTC CCTCCACGAG AAAAAGAGTC ACGGAGAGCA
    GCAGCAAAAA CCAAATTCAC AATGGATTTG GATTCAGATG AAGATTTTTC AGATTTTGAT
    GAAGACACTC AAGATGATTT TGTCCCATCA AATGCTAGTC CACCTAAGAT CCAAACTTCC
    CCGAAACATA ATAACAAAGA ACTGAAGCCA CTGAAGAGTG CCTCATCAGT AACAGAGCTT
    GATGCTGATG ATGCTGAGGA TAATGCACCA CCTCCTCCAA GCTCTCCTGC TGCAGATTTC
    CCAGCTGTAA CTGAAACCAT AAACCCTGTT CCTAAAAAGA ATGTGACAGC AAAGAAGACA
    GCAGCAAAAA GTCAGTCTTC CACCTCCACT ACAGGCACCA AAAAAAGGGC TGCACCAAAA
    AAAACCAAAA AGGATCCAGA CTTGGATTCT GATGTCTCTA AAAAGCCTAA TGCCCCCAAA
    ACCAAGACTC GCCGCAAAAG GAAGGCGTCC TCCTCTGATG ATTCCGACTC TAATTTTGAG
    AAACTGATCT CCAAAGCAGT CACAAGCAAG AAACCCAAGG AGGAAAGTGA TGATTTCCAT
    CTGGACTTGG ACTCAGCTGT GGGTTCTCGG GCAAAATCTG TAAGAACAAA GAAACCTATA
    AAGTACCTTG AAGAATCAGA TGAAGATGAT TTCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58213
    Clone ID Related Accession (Same CDS sequence) XM_010824139.1 , XM_010816273.1
    Accession Version XM_010824139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646463.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTATGTCC CAGCTCTTAT CTTTGGACAG CTCCTAACTT CTAGCAACTA TGATGATGAA 
    GAAAAGAAAG TAACAGGTGG TCGAAATGGC TATGGAGCCA AACTGTGTAA CATATTCAGT
    ACCAAATTTA CTGTGGAAAC AGCAAGTAGA GAATACAAGA AAATGTTCAA ACAGACATGG
    ATGGATAACA TGGGGAAAGC TGGTGAGATG GAACTCAAGC CCTTTAGTGG AGAAGATTAT
    ACATGTATTA CCTTCCACCC TGATTTGTCC AAGTTTAAAA TGCAAAGCCT AGACAAAGAT
    ATTGTTGCAC TGATGGTTAG AAGAGCATAT GATATTGCTG GATCCACTAA AGATGTCAAA
    GTCTATCTCA ATGGAAATAG GCTTCCAGTG AAAGGATTCC GTAGCTATGT AGATCTGTAT
    CTGAAGGATA AGGTAGATGA AACTGGCAAT CCATTGAAAG TTATCCATGA ACAAGTAAAC
    CACAGGTGGG AAGTATGTTT AACAATGAGT GAAAAAGGCT TTCAGCAAAT TAGCTTTGTC
    AACAGCATTG CTACTTCCAA GGGTGGCCGA CATGTTGATT ATGTAGCTGA TCAGATTGTG
    ACTAAACTTG TTGATGTTGT GAAGAAAAAG AACAAGGGTG GTGTTGCAGT AAAAGCACAT
    CAGGTAAAAA ATCACATGTG GATTTTTGTG AATGCCTTAA TTGAAAACCC AACCTTCGAC
    TCCCAGACAA AAGAGAACAT GACTTTACAA GTCAAGAGCT TTGGATCAAC ATGCCAGCTG
    AGTGAAAAAT TCATCAAAGC TGCGATTGGC TGTGGTATTG TAGAAAGTAT ACTAAATTGG
    GTGAAATTTA AGGCCCAAAT CCAGTTAAAC AAGAAGTGTT CTGCTGTAAA ACATAACAGA
    ATCAAAGGAA TTCCCAAACT TGATGATGCC AATGATGCAG GAGGCCGAAA CTCTACTGAG
    TGTACACTTA TCCTGACAGA GGGAGACTCA GCTAAAACTT TGGCTGTTTC AGGCCTTGGT
    GTGGTTGGGA GAGACAAGTA TGGGGTTTTC CCTCTTAGAG GAAAAATCCT CAATGTTCGA
    GAAGCTTCTC ATAAACAGAT AATGGAAAAT GCTGAAATTA ACAATATAAT TAAGATTGTG
    GGTCTTCAGT ACAAGAAAAA CTATGAAGAT GAAGATTCAC TGAAGACTCT ACGTTATGGA
    AAGATAATGA TTATGACAGA TCAGGACCAA GACGGTTCCC ATATCAAAGG CTTGCTGATT
    AATTTTATCC ATCACAACTG GCCCTCTCTT CTGCGACATC GTTTTCTGGA GGAATTTATC
    ACTCCCATTG TAAAGGTGTC TAAAAACAAA CAAGAAATGG CATTCTATAG CCTTCCTGAG
    TTTGAAGAAT GGAAGAGTTC TACACCAAAT CATAAAAAAT GGAAAGTCAA ATATTACAAA
    GGTTTGGGTA CCAGCACATC AAAGGAAGCT AAAGAATACT TTGCAGATAT GAAAAGACAT
    CGTATCCAAT TTAAATATTC CGGTCCCGAA GATGATGCTG CAATCAGCCT GGCCTTTAGC
    AAAAAGCAGA CAGATGATCG AAAGGAATGG TTAACTCATT TCATGGAAGA TAGAAGACAA
    CGAAAGTTAC TTGGCCTTCC TGATGATTAT TTGTATGGGC AAGCTACCAC ATATCTGACA
    TACAATGACT TCATAAACAA GGAACTTATC CTGTTTTCAA ATTCTGATAA TGAAAGGTCT
    ATCCCATCTA TGGTGGATGG TTTGAAACCA GGTCAGAGAA AGGTTTTGTT TACATGTTTC
    AAACGGAATG ACAAGCGAGA GGTGAAGGTT GCCCAGTTAG CTGGGTCAGT AGCTGAGATG
    TCCTCTTATC ATCATGGTGA GATGTCACTA ATGATGACCA TTATCAATTT GGCTCAGAAC
    TTTGTGGGCA GCAATAATCT GAACCTTTTG CAGCCCATTG GTCAGTTTGG TACCAGGTTG
    CATGGTGGCA AGGACTCTGC TAGTCCTCGA TACATCTTCA CAATGCTCAG CCCTTTGGCT
    CGGTTGTTAT TTCCAGCAAA AGATGACCAT ACATTAAAGT TTTTATATGA TGACAACCAG
    CGTGTTGAGC CTGAATGGTA TATTCCTATT ATACCCATGG TGTTGATAAA TGGCGCTGAA
    GGAATTGGTA CTGGGTGGTC CTGCAAAATC CCCAACTTTG ATGTTCGTGA AGTTGTGAAT
    AACATCAGAC GTTTGATGGA TGGAGAAGAA CCTTTGCCAA TGCTTCCAAG TTACAAGAAC
    TTCAAGGGTA CCGTTGAGGA GCTGGCTCCA AATCAATATG TGATTAGTGG TGAAGTGGCT
    ATTCTTAATT CTACAACCAT TGAAATCTCA GAGCTTCCCA TCAGAACGTG GACCCAGACG
    TATAAGGAAC AGGTTCTTGA GCCCATGTTG AATGGCACTG AGAAGACACC GCCTCTCATA
    ACAGATTATA GGGAATACCA CACAGACACC ACTGTGAAGT TTGTTGTGAA GATGACTGAA
    GAAAAGCTGG CGGAGGCAGA GAGAGCTGGA CTACACAAAG TCTTCAAACT CCAAACTAGT
    CTCACATGCA ACTCTATGGT CCTCTTTGAC CATGTAGGCT GTTTAAAGAA ATATGACACA
    GTTTTGGATA TTCTAAGAGA CTTCTTTGAA CTCAGGCTTA AATATTATGG ATTAAGAAAA
    GAATGGCTTC TAGGAATGCT TGGTGCTGAA TCTGCTAAAC TGAATAATCA GGCTCGCTTT
    ATCTTAGAGA AAATAGATGG CAAAATAATC ATTGAAAATA AGCCTAAGAA AGAGTTAATT
    AAAGTTCTGA TTCAGAGAGG ATATGATTCG GATCCTGTGA AGGCTTGGAA AGAAGCCCAG
    CAAAAGGTTC CGGATGAAGA AGAAAATGAA GAAAGTGACA GTGAAAAGGA AACTGAAAAG
    AGTGACTCTG CAACAGATTC TGGACCTACC TTCAACTACC TTCTTGACAT GCCTCTTTGG
    TATCTAACCA AGGAAAAGAA AGATGAACTG TGCAAACTAA GAAATGAAAA AGAGCAAGAG
    CTAGAAACAT TGAAGAGAAA GAGTCCATCA GATTTGTGGA AAGAAGACTT GGCTGCTTTT
    GTTGAAGAGC TGGAGGTTGT TGAAGCAAAG GAGAAACAAG ATGAACAAAT AGGACTTCCT
    GGGAAAGGTG GGAAAGCAAA GGGGAAAAAA ACACAAATGG TAGAAATTTT GCCTTCTCCA
    AGTGGAAAAA GAGTCATCCC CCGAGTGACT GAAGAAATGA AAGCAGAGGC AGAAAAGAAA
    ATTAAAAAGA AAATTAAGAG TGAAAATACT GAAGGAAGCC CTCAAGAGGA TGGAATGGAA
    ATGGAAAGCC AAAAACTGAG ACTGGAAAAG AAACAGAAAA GAGAGCCAGT TACCAAGACA
    AAGAAACAAA CTACATTGCC ATTTAAACCA ATCAAGAAAG CAAAGAAAAG AAACCCCTGG
    TCCGATTCAG AATCAGATAT GAGCAGTAAC GAAAGTAATT TTGATGTCCC TCCACGAGAA
    AAAGAGTCAC GGAGAGCAGC AGCAAAAACC AAATTCACAA TGGATTTGGA TTCAGATGAA
    GATTTTTCAG ATTTTGATGA AGACACTCAA GATGATTTTG TCCCATCAAA TGCTAGTCCA
    CCTAAGATCC AAACTTCCCC GAAACATAAT AACAAAGAAC TGAAGCCACT GAAGAGTGCC
    TCATCAGTAA CAGAGCTTGA TGCTGATGAT GCTGAGGATA ATGCACCACC TCCTCCAAGC
    TCTCCTGCTG CAGATTTCCC AGCTGTAACT GAAACCATAA ACCCTGTTCC TAAAAAGAAT
    GTGACAGCAA AGAAGACAGC AGCAAAAAGT CAGTCTTCCA CCTCCACTAC AGGCACCAAA
    AAAAGGGCTG CACCAAAAAA AACCAAAAAG GATCCAGACT TGGATTCTGA TGTCTCTAAA
    AAGCCTAATG CCCCCAAAAC CAAGACTCGC CGCAAAAGGA AGGCGTCCTC CTCTGATGAT
    TCCGACTCTA ATTTTGAGAA ACTGATCTCC AAAGCAGTCA CAAGCAAGAA ACCCAAGGAG
    GAAAGTGATG ATTTCCATCT GGACTTGGAC TCAGCTGTGG GTTCTCGGGC AAAATCTGTA
    AGAACAAAGA AACCTATAAA GTACCTTGAA GAATCAGATG AAGATGATTT CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822441.1
    CDS143..4339
    Translation

    Target ORF information:

    RefSeq Version XM_010824139.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTATGTCC CAGCTCTTAT CTTTGGACAG CTCCTAACTT CTAGCAACTA TGATGATGAA 
    GAAAAGAAAG TAACAGGTGG TCGAAATGGC TATGGAGCCA AACTGTGTAA CATATTCAGT
    ACCAAATTTA CTGTGGAAAC AGCAAGTAGA GAATACAAGA AAATGTTCAA ACAGACATGG
    ATGGATAACA TGGGGAAAGC TGGTGAGATG GAACTCAAGC CCTTTAGTGG AGAAGATTAT
    ACATGTATTA CCTTCCACCC TGATTTGTCC AAGTTTAAAA TGCAAAGCCT AGACAAAGAT
    ATTGTTGCAC TGATGGTTAG AAGAGCATAT GATATTGCTG GATCCACTAA AGATGTCAAA
    GTCTATCTCA ATGGAAATAG GCTTCCAGTG AAAGGATTCC GTAGCTATGT AGATCTGTAT
    CTGAAGGATA AGGTAGATGA AACTGGCAAT CCATTGAAAG TTATCCATGA ACAAGTAAAC
    CACAGGTGGG AAGTATGTTT AACAATGAGT GAAAAAGGCT TTCAGCAAAT TAGCTTTGTC
    AACAGCATTG CTACTTCCAA GGGTGGCCGA CATGTTGATT ATGTAGCTGA TCAGATTGTG
    ACTAAACTTG TTGATGTTGT GAAGAAAAAG AACAAGGGTG GTGTTGCAGT AAAAGCACAT
    CAGGTAAAAA ATCACATGTG GATTTTTGTG AATGCCTTAA TTGAAAACCC AACCTTCGAC
    TCCCAGACAA AAGAGAACAT GACTTTACAA GTCAAGAGCT TTGGATCAAC ATGCCAGCTG
    AGTGAAAAAT TCATCAAAGC TGCGATTGGC TGTGGTATTG TAGAAAGTAT ACTAAATTGG
    GTGAAATTTA AGGCCCAAAT CCAGTTAAAC AAGAAGTGTT CTGCTGTAAA ACATAACAGA
    ATCAAAGGAA TTCCCAAACT TGATGATGCC AATGATGCAG GAGGCCGAAA CTCTACTGAG
    TGTACACTTA TCCTGACAGA GGGAGACTCA GCTAAAACTT TGGCTGTTTC AGGCCTTGGT
    GTGGTTGGGA GAGACAAGTA TGGGGTTTTC CCTCTTAGAG GAAAAATCCT CAATGTTCGA
    GAAGCTTCTC ATAAACAGAT AATGGAAAAT GCTGAAATTA ACAATATAAT TAAGATTGTG
    GGTCTTCAGT ACAAGAAAAA CTATGAAGAT GAAGATTCAC TGAAGACTCT ACGTTATGGA
    AAGATAATGA TTATGACAGA TCAGGACCAA GACGGTTCCC ATATCAAAGG CTTGCTGATT
    AATTTTATCC ATCACAACTG GCCCTCTCTT CTGCGACATC GTTTTCTGGA GGAATTTATC
    ACTCCCATTG TAAAGGTGTC TAAAAACAAA CAAGAAATGG CATTCTATAG CCTTCCTGAG
    TTTGAAGAAT GGAAGAGTTC TACACCAAAT CATAAAAAAT GGAAAGTCAA ATATTACAAA
    GGTTTGGGTA CCAGCACATC AAAGGAAGCT AAAGAATACT TTGCAGATAT GAAAAGACAT
    CGTATCCAAT TTAAATATTC CGGTCCCGAA GATGATGCTG CAATCAGCCT GGCCTTTAGC
    AAAAAGCAGA CAGATGATCG AAAGGAATGG TTAACTCATT TCATGGAAGA TAGAAGACAA
    CGAAAGTTAC TTGGCCTTCC TGATGATTAT TTGTATGGGC AAGCTACCAC ATATCTGACA
    TACAATGACT TCATAAACAA GGAACTTATC CTGTTTTCAA ATTCTGATAA TGAAAGGTCT
    ATCCCATCTA TGGTGGATGG TTTGAAACCA GGTCAGAGAA AGGTTTTGTT TACATGTTTC
    AAACGGAATG ACAAGCGAGA GGTGAAGGTT GCCCAGTTAG CTGGGTCAGT AGCTGAGATG
    TCCTCTTATC ATCATGGTGA GATGTCACTA ATGATGACCA TTATCAATTT GGCTCAGAAC
    TTTGTGGGCA GCAATAATCT GAACCTTTTG CAGCCCATTG GTCAGTTTGG TACCAGGTTG
    CATGGTGGCA AGGACTCTGC TAGTCCTCGA TACATCTTCA CAATGCTCAG CCCTTTGGCT
    CGGTTGTTAT TTCCAGCAAA AGATGACCAT ACATTAAAGT TTTTATATGA TGACAACCAG
    CGTGTTGAGC CTGAATGGTA TATTCCTATT ATACCCATGG TGTTGATAAA TGGCGCTGAA
    GGAATTGGTA CTGGGTGGTC CTGCAAAATC CCCAACTTTG ATGTTCGTGA AGTTGTGAAT
    AACATCAGAC GTTTGATGGA TGGAGAAGAA CCTTTGCCAA TGCTTCCAAG TTACAAGAAC
    TTCAAGGGTA CCGTTGAGGA GCTGGCTCCA AATCAATATG TGATTAGTGG TGAAGTGGCT
    ATTCTTAATT CTACAACCAT TGAAATCTCA GAGCTTCCCA TCAGAACGTG GACCCAGACG
    TATAAGGAAC AGGTTCTTGA GCCCATGTTG AATGGCACTG AGAAGACACC GCCTCTCATA
    ACAGATTATA GGGAATACCA CACAGACACC ACTGTGAAGT TTGTTGTGAA GATGACTGAA
    GAAAAGCTGG CGGAGGCAGA GAGAGCTGGA CTACACAAAG TCTTCAAACT CCAAACTAGT
    CTCACATGCA ACTCTATGGT CCTCTTTGAC CATGTAGGCT GTTTAAAGAA ATATGACACA
    GTTTTGGATA TTCTAAGAGA CTTCTTTGAA CTCAGGCTTA AATATTATGG ATTAAGAAAA
    GAATGGCTTC TAGGAATGCT TGGTGCTGAA TCTGCTAAAC TGAATAATCA GGCTCGCTTT
    ATCTTAGAGA AAATAGATGG CAAAATAATC ATTGAAAATA AGCCTAAGAA AGAGTTAATT
    AAAGTTCTGA TTCAGAGAGG ATATGATTCG GATCCTGTGA AGGCTTGGAA AGAAGCCCAG
    CAAAAGGTTC CGGATGAAGA AGAAAATGAA GAAAGTGACA GTGAAAAGGA AACTGAAAAG
    AGTGACTCTG CAACAGATTC TGGACCTACC TTCAACTACC TTCTTGACAT GCCTCTTTGG
    TATCTAACCA AGGAAAAGAA AGATGAACTG TGCAAACTAA GAAATGAAAA AGAGCAAGAG
    CTAGAAACAT TGAAGAGAAA GAGTCCATCA GATTTGTGGA AAGAAGACTT GGCTGCTTTT
    GTTGAAGAGC TGGAGGTTGT TGAAGCAAAG GAGAAACAAG ATGAACAAAT AGGACTTCCT
    GGGAAAGGTG GGAAAGCAAA GGGGAAAAAA ACACAAATGG TAGAAATTTT GCCTTCTCCA
    AGTGGAAAAA GAGTCATCCC CCGAGTGACT GAAGAAATGA AAGCAGAGGC AGAAAAGAAA
    ATTAAAAAGA AAATTAAGAG TGAAAATACT GAAGGAAGCC CTCAAGAGGA TGGAATGGAA
    ATGGAAAGCC AAAAACTGAG ACTGGAAAAG AAACAGAAAA GAGAGCCAGT TACCAAGACA
    AAGAAACAAA CTACATTGCC ATTTAAACCA ATCAAGAAAG CAAAGAAAAG AAACCCCTGG
    TCCGATTCAG AATCAGATAT GAGCAGTAAC GAAAGTAATT TTGATGTCCC TCCACGAGAA
    AAAGAGTCAC GGAGAGCAGC AGCAAAAACC AAATTCACAA TGGATTTGGA TTCAGATGAA
    GATTTTTCAG ATTTTGATGA AGACACTCAA GATGATTTTG TCCCATCAAA TGCTAGTCCA
    CCTAAGATCC AAACTTCCCC GAAACATAAT AACAAAGAAC TGAAGCCACT GAAGAGTGCC
    TCATCAGTAA CAGAGCTTGA TGCTGATGAT GCTGAGGATA ATGCACCACC TCCTCCAAGC
    TCTCCTGCTG CAGATTTCCC AGCTGTAACT GAAACCATAA ACCCTGTTCC TAAAAAGAAT
    GTGACAGCAA AGAAGACAGC AGCAAAAAGT CAGTCTTCCA CCTCCACTAC AGGCACCAAA
    AAAAGGGCTG CACCAAAAAA AACCAAAAAG GATCCAGACT TGGATTCTGA TGTCTCTAAA
    AAGCCTAATG CCCCCAAAAC CAAGACTCGC CGCAAAAGGA AGGCGTCCTC CTCTGATGAT
    TCCGACTCTA ATTTTGAGAA ACTGATCTCC AAAGCAGTCA CAAGCAAGAA ACCCAAGGAG
    GAAAGTGATG ATTTCCATCT GGACTTGGAC TCAGCTGTGG GTTCTCGGGC AAAATCTGTA
    AGAACAAAGA AACCTATAAA GTACCTTGAA GAATCAGATG AAGATGATTT CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa58213
    Clone ID Related Accession (Same CDS sequence) XM_010824139.1 , XM_010816273.1
    Accession Version XM_010816273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4197bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104496.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTATGTCC CAGCTCTTAT CTTTGGACAG CTCCTAACTT CTAGCAACTA TGATGATGAA 
    GAAAAGAAAG TAACAGGTGG TCGAAATGGC TATGGAGCCA AACTGTGTAA CATATTCAGT
    ACCAAATTTA CTGTGGAAAC AGCAAGTAGA GAATACAAGA AAATGTTCAA ACAGACATGG
    ATGGATAACA TGGGGAAAGC TGGTGAGATG GAACTCAAGC CCTTTAGTGG AGAAGATTAT
    ACATGTATTA CCTTCCACCC TGATTTGTCC AAGTTTAAAA TGCAAAGCCT AGACAAAGAT
    ATTGTTGCAC TGATGGTTAG AAGAGCATAT GATATTGCTG GATCCACTAA AGATGTCAAA
    GTCTATCTCA ATGGAAATAG GCTTCCAGTG AAAGGATTCC GTAGCTATGT AGATCTGTAT
    CTGAAGGATA AGGTAGATGA AACTGGCAAT CCATTGAAAG TTATCCATGA ACAAGTAAAC
    CACAGGTGGG AAGTATGTTT AACAATGAGT GAAAAAGGCT TTCAGCAAAT TAGCTTTGTC
    AACAGCATTG CTACTTCCAA GGGTGGCCGA CATGTTGATT ATGTAGCTGA TCAGATTGTG
    ACTAAACTTG TTGATGTTGT GAAGAAAAAG AACAAGGGTG GTGTTGCAGT AAAAGCACAT
    CAGGTAAAAA ATCACATGTG GATTTTTGTG AATGCCTTAA TTGAAAACCC AACCTTCGAC
    TCCCAGACAA AAGAGAACAT GACTTTACAA GTCAAGAGCT TTGGATCAAC ATGCCAGCTG
    AGTGAAAAAT TCATCAAAGC TGCGATTGGC TGTGGTATTG TAGAAAGTAT ACTAAATTGG
    GTGAAATTTA AGGCCCAAAT CCAGTTAAAC AAGAAGTGTT CTGCTGTAAA ACATAACAGA
    ATCAAAGGAA TTCCCAAACT TGATGATGCC AATGATGCAG GAGGCCGAAA CTCTACTGAG
    TGTACACTTA TCCTGACAGA GGGAGACTCA GCTAAAACTT TGGCTGTTTC AGGCCTTGGT
    GTGGTTGGGA GAGACAAGTA TGGGGTTTTC CCTCTTAGAG GAAAAATCCT CAATGTTCGA
    GAAGCTTCTC ATAAACAGAT AATGGAAAAT GCTGAAATTA ACAATATAAT TAAGATTGTG
    GGTCTTCAGT ACAAGAAAAA CTATGAAGAT GAAGATTCAC TGAAGACTCT ACGTTATGGA
    AAGATAATGA TTATGACAGA TCAGGACCAA GACGGTTCCC ATATCAAAGG CTTGCTGATT
    AATTTTATCC ATCACAACTG GCCCTCTCTT CTGCGACATC GTTTTCTGGA GGAATTTATC
    ACTCCCATTG TAAAGGTGTC TAAAAACAAA CAAGAAATGG CATTCTATAG CCTTCCTGAG
    TTTGAAGAAT GGAAGAGTTC TACACCAAAT CATAAAAAAT GGAAAGTCAA ATATTACAAA
    GGTTTGGGTA CCAGCACATC AAAGGAAGCT AAAGAATACT TTGCAGATAT GAAAAGACAT
    CGTATCCAAT TTAAATATTC CGGTCCCGAA GATGATGCTG CAATCAGCCT GGCCTTTAGC
    AAAAAGCAGA CAGATGATCG AAAGGAATGG TTAACTCATT TCATGGAAGA TAGAAGACAA
    CGAAAGTTAC TTGGCCTTCC TGATGATTAT TTGTATGGGC AAGCTACCAC ATATCTGACA
    TACAATGACT TCATAAACAA GGAACTTATC CTGTTTTCAA ATTCTGATAA TGAAAGGTCT
    ATCCCATCTA TGGTGGATGG TTTGAAACCA GGTCAGAGAA AGGTTTTGTT TACATGTTTC
    AAACGGAATG ACAAGCGAGA GGTGAAGGTT GCCCAGTTAG CTGGGTCAGT AGCTGAGATG
    TCCTCTTATC ATCATGGTGA GATGTCACTA ATGATGACCA TTATCAATTT GGCTCAGAAC
    TTTGTGGGCA GCAATAATCT GAACCTTTTG CAGCCCATTG GTCAGTTTGG TACCAGGTTG
    CATGGTGGCA AGGACTCTGC TAGTCCTCGA TACATCTTCA CAATGCTCAG CCCTTTGGCT
    CGGTTGTTAT TTCCAGCAAA AGATGACCAT ACATTAAAGT TTTTATATGA TGACAACCAG
    CGTGTTGAGC CTGAATGGTA TATTCCTATT ATACCCATGG TGTTGATAAA TGGCGCTGAA
    GGAATTGGTA CTGGGTGGTC CTGCAAAATC CCCAACTTTG ATGTTCGTGA AGTTGTGAAT
    AACATCAGAC GTTTGATGGA TGGAGAAGAA CCTTTGCCAA TGCTTCCAAG TTACAAGAAC
    TTCAAGGGTA CCGTTGAGGA GCTGGCTCCA AATCAATATG TGATTAGTGG TGAAGTGGCT
    ATTCTTAATT CTACAACCAT TGAAATCTCA GAGCTTCCCA TCAGAACGTG GACCCAGACG
    TATAAGGAAC AGGTTCTTGA GCCCATGTTG AATGGCACTG AGAAGACACC GCCTCTCATA
    ACAGATTATA GGGAATACCA CACAGACACC ACTGTGAAGT TTGTTGTGAA GATGACTGAA
    GAAAAGCTGG CGGAGGCAGA GAGAGCTGGA CTACACAAAG TCTTCAAACT CCAAACTAGT
    CTCACATGCA ACTCTATGGT CCTCTTTGAC CATGTAGGCT GTTTAAAGAA ATATGACACA
    GTTTTGGATA TTCTAAGAGA CTTCTTTGAA CTCAGGCTTA AATATTATGG ATTAAGAAAA
    GAATGGCTTC TAGGAATGCT TGGTGCTGAA TCTGCTAAAC TGAATAATCA GGCTCGCTTT
    ATCTTAGAGA AAATAGATGG CAAAATAATC ATTGAAAATA AGCCTAAGAA AGAGTTAATT
    AAAGTTCTGA TTCAGAGAGG ATATGATTCG GATCCTGTGA AGGCTTGGAA AGAAGCCCAG
    CAAAAGGTTC CGGATGAAGA AGAAAATGAA GAAAGTGACA GTGAAAAGGA AACTGAAAAG
    AGTGACTCTG CAACAGATTC TGGACCTACC TTCAACTACC TTCTTGACAT GCCTCTTTGG
    TATCTAACCA AGGAAAAGAA AGATGAACTG TGCAAACTAA GAAATGAAAA AGAGCAAGAG
    CTAGAAACAT TGAAGAGAAA GAGTCCATCA GATTTGTGGA AAGAAGACTT GGCTGCTTTT
    GTTGAAGAGC TGGAGGTTGT TGAAGCAAAG GAGAAACAAG ATGAACAAAT AGGACTTCCT
    GGGAAAGGTG GGAAAGCAAA GGGGAAAAAA ACACAAATGG TAGAAATTTT GCCTTCTCCA
    AGTGGAAAAA GAGTCATCCC CCGAGTGACT GAAGAAATGA AAGCAGAGGC AGAAAAGAAA
    ATTAAAAAGA AAATTAAGAG TGAAAATACT GAAGGAAGCC CTCAAGAGGA TGGAATGGAA
    ATGGAAAGCC AAAAACTGAG ACTGGAAAAG AAACAGAAAA GAGAGCCAGT TACCAAGACA
    AAGAAACAAA CTACATTGCC ATTTAAACCA ATCAAGAAAG CAAAGAAAAG AAACCCCTGG
    TCCGATTCAG AATCAGATAT GAGCAGTAAC GAAAGTAATT TTGATGTCCC TCCACGAGAA
    AAAGAGTCAC GGAGAGCAGC AGCAAAAACC AAATTCACAA TGGATTTGGA TTCAGATGAA
    GATTTTTCAG ATTTTGATGA AGACACTCAA GATGATTTTG TCCCATCAAA TGCTAGTCCA
    CCTAAGATCC AAACTTCCCC GAAACATAAT AACAAAGAAC TGAAGCCACT GAAGAGTGCC
    TCATCAGTAA CAGAGCTTGA TGCTGATGAT GCTGAGGATA ATGCACCACC TCCTCCAAGC
    TCTCCTGCTG CAGATTTCCC AGCTGTAACT GAAACCATAA ACCCTGTTCC TAAAAAGAAT
    GTGACAGCAA AGAAGACAGC AGCAAAAAGT CAGTCTTCCA CCTCCACTAC AGGCACCAAA
    AAAAGGGCTG CACCAAAAAA AACCAAAAAG GATCCAGACT TGGATTCTGA TGTCTCTAAA
    AAGCCTAATG CCCCCAAAAC CAAGACTCGC CGCAAAAGGA AGGCGTCCTC CTCTGATGAT
    TCCGACTCTA ATTTTGAGAA ACTGATCTCC AAAGCAGTCA CAAGCAAGAA ACCCAAGGAG
    GAAAGTGATG ATTTCCATCT GGACTTGGAC TCAGCTGTGG GTTCTCGGGC AAAATCTGTA
    AGAACAAAGA AACCTATAAA GTACCTTGAA GAATCAGATG AAGATGATTT CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814575.1
    CDS143..4339
    Translation

    Target ORF information:

    RefSeq Version XM_010816273.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II alpha (TOP2A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTATGTCC CAGCTCTTAT CTTTGGACAG CTCCTAACTT CTAGCAACTA TGATGATGAA 
    GAAAAGAAAG TAACAGGTGG TCGAAATGGC TATGGAGCCA AACTGTGTAA CATATTCAGT
    ACCAAATTTA CTGTGGAAAC AGCAAGTAGA GAATACAAGA AAATGTTCAA ACAGACATGG
    ATGGATAACA TGGGGAAAGC TGGTGAGATG GAACTCAAGC CCTTTAGTGG AGAAGATTAT
    ACATGTATTA CCTTCCACCC TGATTTGTCC AAGTTTAAAA TGCAAAGCCT AGACAAAGAT
    ATTGTTGCAC TGATGGTTAG AAGAGCATAT GATATTGCTG GATCCACTAA AGATGTCAAA
    GTCTATCTCA ATGGAAATAG GCTTCCAGTG AAAGGATTCC GTAGCTATGT AGATCTGTAT
    CTGAAGGATA AGGTAGATGA AACTGGCAAT CCATTGAAAG TTATCCATGA ACAAGTAAAC
    CACAGGTGGG AAGTATGTTT AACAATGAGT GAAAAAGGCT TTCAGCAAAT TAGCTTTGTC
    AACAGCATTG CTACTTCCAA GGGTGGCCGA CATGTTGATT ATGTAGCTGA TCAGATTGTG
    ACTAAACTTG TTGATGTTGT GAAGAAAAAG AACAAGGGTG GTGTTGCAGT AAAAGCACAT
    CAGGTAAAAA ATCACATGTG GATTTTTGTG AATGCCTTAA TTGAAAACCC AACCTTCGAC
    TCCCAGACAA AAGAGAACAT GACTTTACAA GTCAAGAGCT TTGGATCAAC ATGCCAGCTG
    AGTGAAAAAT TCATCAAAGC TGCGATTGGC TGTGGTATTG TAGAAAGTAT ACTAAATTGG
    GTGAAATTTA AGGCCCAAAT CCAGTTAAAC AAGAAGTGTT CTGCTGTAAA ACATAACAGA
    ATCAAAGGAA TTCCCAAACT TGATGATGCC AATGATGCAG GAGGCCGAAA CTCTACTGAG
    TGTACACTTA TCCTGACAGA GGGAGACTCA GCTAAAACTT TGGCTGTTTC AGGCCTTGGT
    GTGGTTGGGA GAGACAAGTA TGGGGTTTTC CCTCTTAGAG GAAAAATCCT CAATGTTCGA
    GAAGCTTCTC ATAAACAGAT AATGGAAAAT GCTGAAATTA ACAATATAAT TAAGATTGTG
    GGTCTTCAGT ACAAGAAAAA CTATGAAGAT GAAGATTCAC TGAAGACTCT ACGTTATGGA
    AAGATAATGA TTATGACAGA TCAGGACCAA GACGGTTCCC ATATCAAAGG CTTGCTGATT
    AATTTTATCC ATCACAACTG GCCCTCTCTT CTGCGACATC GTTTTCTGGA GGAATTTATC
    ACTCCCATTG TAAAGGTGTC TAAAAACAAA CAAGAAATGG CATTCTATAG CCTTCCTGAG
    TTTGAAGAAT GGAAGAGTTC TACACCAAAT CATAAAAAAT GGAAAGTCAA ATATTACAAA
    GGTTTGGGTA CCAGCACATC AAAGGAAGCT AAAGAATACT TTGCAGATAT GAAAAGACAT
    CGTATCCAAT TTAAATATTC CGGTCCCGAA GATGATGCTG CAATCAGCCT GGCCTTTAGC
    AAAAAGCAGA CAGATGATCG AAAGGAATGG TTAACTCATT TCATGGAAGA TAGAAGACAA
    CGAAAGTTAC TTGGCCTTCC TGATGATTAT TTGTATGGGC AAGCTACCAC ATATCTGACA
    TACAATGACT TCATAAACAA GGAACTTATC CTGTTTTCAA ATTCTGATAA TGAAAGGTCT
    ATCCCATCTA TGGTGGATGG TTTGAAACCA GGTCAGAGAA AGGTTTTGTT TACATGTTTC
    AAACGGAATG ACAAGCGAGA GGTGAAGGTT GCCCAGTTAG CTGGGTCAGT AGCTGAGATG
    TCCTCTTATC ATCATGGTGA GATGTCACTA ATGATGACCA TTATCAATTT GGCTCAGAAC
    TTTGTGGGCA GCAATAATCT GAACCTTTTG CAGCCCATTG GTCAGTTTGG TACCAGGTTG
    CATGGTGGCA AGGACTCTGC TAGTCCTCGA TACATCTTCA CAATGCTCAG CCCTTTGGCT
    CGGTTGTTAT TTCCAGCAAA AGATGACCAT ACATTAAAGT TTTTATATGA TGACAACCAG
    CGTGTTGAGC CTGAATGGTA TATTCCTATT ATACCCATGG TGTTGATAAA TGGCGCTGAA
    GGAATTGGTA CTGGGTGGTC CTGCAAAATC CCCAACTTTG ATGTTCGTGA AGTTGTGAAT
    AACATCAGAC GTTTGATGGA TGGAGAAGAA CCTTTGCCAA TGCTTCCAAG TTACAAGAAC
    TTCAAGGGTA CCGTTGAGGA GCTGGCTCCA AATCAATATG TGATTAGTGG TGAAGTGGCT
    ATTCTTAATT CTACAACCAT TGAAATCTCA GAGCTTCCCA TCAGAACGTG GACCCAGACG
    TATAAGGAAC AGGTTCTTGA GCCCATGTTG AATGGCACTG AGAAGACACC GCCTCTCATA
    ACAGATTATA GGGAATACCA CACAGACACC ACTGTGAAGT TTGTTGTGAA GATGACTGAA
    GAAAAGCTGG CGGAGGCAGA GAGAGCTGGA CTACACAAAG TCTTCAAACT CCAAACTAGT
    CTCACATGCA ACTCTATGGT CCTCTTTGAC CATGTAGGCT GTTTAAAGAA ATATGACACA
    GTTTTGGATA TTCTAAGAGA CTTCTTTGAA CTCAGGCTTA AATATTATGG ATTAAGAAAA
    GAATGGCTTC TAGGAATGCT TGGTGCTGAA TCTGCTAAAC TGAATAATCA GGCTCGCTTT
    ATCTTAGAGA AAATAGATGG CAAAATAATC ATTGAAAATA AGCCTAAGAA AGAGTTAATT
    AAAGTTCTGA TTCAGAGAGG ATATGATTCG GATCCTGTGA AGGCTTGGAA AGAAGCCCAG
    CAAAAGGTTC CGGATGAAGA AGAAAATGAA GAAAGTGACA GTGAAAAGGA AACTGAAAAG
    AGTGACTCTG CAACAGATTC TGGACCTACC TTCAACTACC TTCTTGACAT GCCTCTTTGG
    TATCTAACCA AGGAAAAGAA AGATGAACTG TGCAAACTAA GAAATGAAAA AGAGCAAGAG
    CTAGAAACAT TGAAGAGAAA GAGTCCATCA GATTTGTGGA AAGAAGACTT GGCTGCTTTT
    GTTGAAGAGC TGGAGGTTGT TGAAGCAAAG GAGAAACAAG ATGAACAAAT AGGACTTCCT
    GGGAAAGGTG GGAAAGCAAA GGGGAAAAAA ACACAAATGG TAGAAATTTT GCCTTCTCCA
    AGTGGAAAAA GAGTCATCCC CCGAGTGACT GAAGAAATGA AAGCAGAGGC AGAAAAGAAA
    ATTAAAAAGA AAATTAAGAG TGAAAATACT GAAGGAAGCC CTCAAGAGGA TGGAATGGAA
    ATGGAAAGCC AAAAACTGAG ACTGGAAAAG AAACAGAAAA GAGAGCCAGT TACCAAGACA
    AAGAAACAAA CTACATTGCC ATTTAAACCA ATCAAGAAAG CAAAGAAAAG AAACCCCTGG
    TCCGATTCAG AATCAGATAT GAGCAGTAAC GAAAGTAATT TTGATGTCCC TCCACGAGAA
    AAAGAGTCAC GGAGAGCAGC AGCAAAAACC AAATTCACAA TGGATTTGGA TTCAGATGAA
    GATTTTTCAG ATTTTGATGA AGACACTCAA GATGATTTTG TCCCATCAAA TGCTAGTCCA
    CCTAAGATCC AAACTTCCCC GAAACATAAT AACAAAGAAC TGAAGCCACT GAAGAGTGCC
    TCATCAGTAA CAGAGCTTGA TGCTGATGAT GCTGAGGATA ATGCACCACC TCCTCCAAGC
    TCTCCTGCTG CAGATTTCCC AGCTGTAACT GAAACCATAA ACCCTGTTCC TAAAAAGAAT
    GTGACAGCAA AGAAGACAGC AGCAAAAAGT CAGTCTTCCA CCTCCACTAC AGGCACCAAA
    AAAAGGGCTG CACCAAAAAA AACCAAAAAG GATCCAGACT TGGATTCTGA TGTCTCTAAA
    AAGCCTAATG CCCCCAAAAC CAAGACTCGC CGCAAAAGGA AGGCGTCCTC CTCTGATGAT
    TCCGACTCTA ATTTTGAGAA ACTGATCTCC AAAGCAGTCA CAAGCAAGAA ACCCAAGGAG
    GAAAGTGATG ATTTCCATCT GGACTTGGAC TCAGCTGTGG GTTCTCGGGC AAAATCTGTA
    AGAACAAAGA AACCTATAAA GTACCTTGAA GAATCAGATG AAGATGATTT CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.