CRB2 cDNA ORF clone, Bos taurus(cattle)

The following CRB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa220930 XM_003586670.5
Latest version!
Bos taurus crumbs 2, cell polarity complex component (CRB2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa24312 XM_003586670.4
Latest version!
Bos taurus crumbs family member 2 (CRB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa24312 XM_015465485.1
Latest version!
Bos taurus crumbs family member 2 (CRB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa24313 XM_005213164.3
Latest version!
Bos taurus crumbs family member 2 (CRB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa24313 XM_015465486.1
Latest version!
Bos taurus crumbs family member 2 (CRB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa220930
    Clone ID Related Accession (Same CDS sequence) XM_003586670.5
    Accession Version XM_003586670.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein crumbs homolog 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586670.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG GGATGACTGC
    GGCGAGGATG TAGATGAGTG TGCCTCTCGG CCGTGCCTCA GTGGAGGCCT CTGCCAGGAC
    CTGCCCAATG GCTTCCACTG CCACTGTCCA GACGGCTACA CAGGCCCAAC GTGTCAGGAA
    GACATGGACG AATGCCTGTC GGAGCCTTGC CTCCATGGTG GGACCTGTGA AGACACCGTG
    GCAGGCTACA TCTGTGGGTG CCCCGAGGCC TGGGGTGGAT TGGATTGTTC TGTGCCACTC
    ACCGGCTGCC AGGGCCACAC TTGCCCACCG ACTGCCACCT GCGTCCCTAC CTTCGAGTCA
    GGGGTTCACA GTTACACCTG CCGCTGCCCA CCTGGTACCC ATGGACCTTT CTGTGGCCAG
    AATACTACAT TCTCCATGGT GGCTGGGAAC CCCTTACGGG CTTCGGTGCC AGCTGGCAGC
    TCCCTAGGTC TTGCCCTGAG GTTTCGTACC ACGATACGTG TGGGTGCCTT GGCCACGTGC
    TCGGACACTC AAGGCAGCGT GGAGCTGGCG CTGGTGGAGG CCAGGCTTCA GGCCACACTC
    TGGAGCCACG GCAAGAATGT GCTCGTCCTG AGGCTGCTGG AACCACCCCT CAATGATGGC
    CACTGGCACC GAGTGGAGGT GACGCTCCGC CTGGGGGTCC TGGAGTTGCG GCTCTGGCAT
    GAAGACTGTC CCGCCCACCT CTGTGTGGCC TCCAGTCCCA TGGCCACGGC TGCCCCGGCC
    CCCACGCCTG CTGGGTCCCG CTTCACCCAG CTGGGCGGCG TGGCCTTTGC GGGCTGCCTC
    CAGGATGTGC AGGTGGATGG GCATCTCCTG CTGCCCGAGG ACCTTGGTGA GAATGTCCTC
    CTGGGCTGCT GGCACCAGGA GCACTGCCAA CCTCCGCCTT GTGCCCACGG AGGGGTCTGC
    GTGGACCTGT GGACTCACTT CCATTGTGAC TGCCCCCGGC CCTACAGAGG TCCCACGTGT
    GCTGATGAGG TTCCTGCTGC CACCTTTGGC TTGGGGGGCA CCCTGAGCTC TGCCTCCTTC
    CTACTCGACC AGCCGCCAGG CCCCAACCTC ACCGTGTCCT TCCTCCTCCG CACCCGGGAA
    CCTGCTGGCC TTCTGCTCCA GCTCACCAAC GGTTCGGCCG CTGGCCTGAC AGTGTTCCTG
    AGCGAGGGCC AGATCTGGGC CGAGGCACTG GGCAGTCCTG CTCTGGTGCT CCCGGGGCGC
    TGGGATGACG GGCTCCGCCA CCTGGTGACA CTCAGCTTCG GGCCTGATCA GCTGTGGGGT
    GTGGGGCAGC AGGTGCACGT GGGCGGCAGG CTCCTCCCCG GTGACGCCGA GCCCTGGGGT
    GGGCCCTTCC GAGGCTGCAT GCAGGACGTG CGACTCAATG ACCTCCACCT CCCCTTCTTT
    CCACCACTGC TGGGGAACTC CAGCCTGCCC AGCGTGCCCG GCCGCGGGCA GTCCTGGAAC
    CTCACCATGG GCTGCATCTC TGAGGACACA TGCAATCCTG AGCCCTGTCT CAATGGTGGG
    ACTTGCCTCG TCACCTGGAA TGACTTCCAC TGCACCTGCC CTGCCAACTT CACTGGGCCC
    ACGTGTGCCC AGCAGCTGTG GTGTCCTGGC CAGCCCTGTC TCCCGCCTGC CACCTGTGAG
    GAGGTCCCTG ATGGCTTTGT CTGTGTGGCG GAGGCCACGT TCCGCGAGGG ACCCCCGGCG
    GAATTCAGCG GGCACAACGC GTCATCGGGC AGCGCTCTCA GCGGCCTCTC GCTGGTCTTC
    CGCACTCGCG ACCCCGAGGC AGGGCTGTTG CGCGCTGAGG ACGGCCCGGC CGCCGTGTGG
    CTGGCGGTGC GCAACGGCTC GCTGGCGGCC GGCGTGCGCA GCGGCGCCGG TTTGCCCCGC
    GCGGTGCTGC CAGCGCCCGG GCCGCGTGTG GCCGACGGCG CCTGGCACCG CGTGCGCCTG
    GCCATGGAGC AGCCCGCGGC CGTCGCTTCG CGCTGGCTGC TCTGGCTGGA CGGCGCGGCG
    ACGCCGGTGT CGCTGCGTGG CCTGGCCGGC GACCTGGACT TCCTGCGCGG CCCGGGCGCC
    GCGCGAGTCC TGCTGGCCGA GAACTTCACG GGCTGCCTGG GCCGCGTGGC GCTCGGCGGC
    CACCCGCTAC CCCTGGCGCG CCCGCGGCCC GGCGCGGCCC CCGGCTCCCG CGAGCCCTTC
    TCGGCCCGGC CCGGAGCGCC CGCCCCGCAC CTCGGCTGCA GCGGCGCTCC CGTGTGTGTC
    CCCTCGCCCT GCCTGCACGG CGGCGCCTGC CGCGACCTCT TCGACGCCTT CGCCTGCGCC
    TGCGGCCCGG GCTGGGAGGG CCCGCGCTGC GAGGACCGCG CCGACCCGTG TCGCTCGGCG
    CCCTGCGCCC GCGGCCGCTG CCACGCACAC CCAGACGGTC ACTTCGAGTG CCGCTGCCCG
    CCTGGCTTCG CAGGCCCGCG CTGCAGGTTG CCTGTCCCAC CAGAGGGGTG CAGCCTGAAC
    ATCACCTGTC TCAACGGTGG CCAGTGTGAG GAGGGGCCCC AGGGGGCCAA CTGCAGCTGC
    CAGGAGAGCT TTGCTGGGCA GAGATGTCAG ATCCCCTGTG AAGCCAACCC CTGCTTGAAT
    GGGGGCACCT GCCGGGCAGC TGATGGGGTG TACGAATGTG TCTGCAATGC CAGGTTCTCA
    GGCCAGTTCT GCGAAGTGGT GAAAGGCCTA CCACTGCCGC TGCCGTTCCC GCTGCTGGAG
    GTAGCAGTGC CCGCGGCCTG CGCCTGCCTC CTCCTCCTCC TCTTGGGCCT CCTCTCAGGG
    ATCCTGGCGG CCAGAAAGCG CCGCCAGTCA GAGGGCACCT ACAGTCCGAG CCAGCAGGAG
    GTGGCCGGAG CCCGGCTGGA GATGGACAGC GTCCTCAAGG TGCCGCCCGA GGAGAGACTC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586718.1
    CDS52..3897
    Translation

    Target ORF information:

    RefSeq Version XM_003586670.5
    Organism Bos taurus(cattle)
    Definition Bos taurus crumbs 2, cell polarity complex component (CRB2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586670.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG GGATGACTGC
    GGCGAGGATG TAGATGAGTG TGCCTCTCGG CCGTGCCTCA GTGGAGGCCT CTGCCAGGAC
    CTGCCCAATG GCTTCCACTG CCACTGTCCA GACGGCTACA CAGGCCCAAC GTGTCAGGAA
    GACATGGACG AATGCCTGTC GGAGCCTTGC CTCCATGGTG GGACCTGTGA AGACACCGTG
    GCAGGCTACA TCTGTGGGTG CCCCGAGGCC TGGGGTGGAT TGGATTGTTC TGTGCCACTC
    ACCGGCTGCC AGGGCCACAC TTGCCCACCG ACTGCCACCT GCGTCCCTAC CTTCGAGTCA
    GGGGTTCACA GTTACACCTG CCGCTGCCCA CCTGGTACCC ATGGACCTTT CTGTGGCCAG
    AATACTACAT TCTCCATGGT GGCTGGGAAC CCCTTACGGG CTTCGGTGCC AGCTGGCAGC
    TCCCTAGGTC TTGCCCTGAG GTTTCGTACC ACGATACGTG TGGGTGCCTT GGCCACGTGC
    TCGGACACTC AAGGCAGCGT GGAGCTGGCG CTGGTGGAGG CCAGGCTTCA GGCCACACTC
    TGGAGCCACG GCAAGAATGT GCTCGTCCTG AGGCTGCTGG AACCACCCCT CAATGATGGC
    CACTGGCACC GAGTGGAGGT GACGCTCCGC CTGGGGGTCC TGGAGTTGCG GCTCTGGCAT
    GAAGACTGTC CCGCCCACCT CTGTGTGGCC TCCAGTCCCA TGGCCACGGC TGCCCCGGCC
    CCCACGCCTG CTGGGTCCCG CTTCACCCAG CTGGGCGGCG TGGCCTTTGC GGGCTGCCTC
    CAGGATGTGC AGGTGGATGG GCATCTCCTG CTGCCCGAGG ACCTTGGTGA GAATGTCCTC
    CTGGGCTGCT GGCACCAGGA GCACTGCCAA CCTCCGCCTT GTGCCCACGG AGGGGTCTGC
    GTGGACCTGT GGACTCACTT CCATTGTGAC TGCCCCCGGC CCTACAGAGG TCCCACGTGT
    GCTGATGAGG TTCCTGCTGC CACCTTTGGC TTGGGGGGCA CCCTGAGCTC TGCCTCCTTC
    CTACTCGACC AGCCGCCAGG CCCCAACCTC ACCGTGTCCT TCCTCCTCCG CACCCGGGAA
    CCTGCTGGCC TTCTGCTCCA GCTCACCAAC GGTTCGGCCG CTGGCCTGAC AGTGTTCCTG
    AGCGAGGGCC AGATCTGGGC CGAGGCACTG GGCAGTCCTG CTCTGGTGCT CCCGGGGCGC
    TGGGATGACG GGCTCCGCCA CCTGGTGACA CTCAGCTTCG GGCCTGATCA GCTGTGGGGT
    GTGGGGCAGC AGGTGCACGT GGGCGGCAGG CTCCTCCCCG GTGACGCCGA GCCCTGGGGT
    GGGCCCTTCC GAGGCTGCAT GCAGGACGTG CGACTCAATG ACCTCCACCT CCCCTTCTTT
    CCACCACTGC TGGGGAACTC CAGCCTGCCC AGCGTGCCCG GCCGCGGGCA GTCCTGGAAC
    CTCACCATGG GCTGCATCTC TGAGGACACA TGCAATCCTG AGCCCTGTCT CAATGGTGGG
    ACTTGCCTCG TCACCTGGAA TGACTTCCAC TGCACCTGCC CTGCCAACTT CACTGGGCCC
    ACGTGTGCCC AGCAGCTGTG GTGTCCTGGC CAGCCCTGTC TCCCGCCTGC CACCTGTGAG
    GAGGTCCCTG ATGGCTTTGT CTGTGTGGCG GAGGCCACGT TCCGCGAGGG ACCCCCGGCG
    GAATTCAGCG GGCACAACGC GTCATCGGGC AGCGCTCTCA GCGGCCTCTC GCTGGTCTTC
    CGCACTCGCG ACCCCGAGGC AGGGCTGTTG CGCGCTGAGG ACGGCCCGGC CGCCGTGTGG
    CTGGCGGTGC GCAACGGCTC GCTGGCGGCC GGCGTGCGCA GCGGCGCCGG TTTGCCCCGC
    GCGGTGCTGC CAGCGCCCGG GCCGCGTGTG GCCGACGGCG CCTGGCACCG CGTGCGCCTG
    GCCATGGAGC AGCCCGCGGC CGTCGCTTCG CGCTGGCTGC TCTGGCTGGA CGGCGCGGCG
    ACGCCGGTGT CGCTGCGTGG CCTGGCCGGC GACCTGGACT TCCTGCGCGG CCCGGGCGCC
    GCGCGAGTCC TGCTGGCCGA GAACTTCACG GGCTGCCTGG GCCGCGTGGC GCTCGGCGGC
    CACCCGCTAC CCCTGGCGCG CCCGCGGCCC GGCGCGGCCC CCGGCTCCCG CGAGCCCTTC
    TCGGCCCGGC CCGGAGCGCC CGCCCCGCAC CTCGGCTGCA GCGGCGCTCC CGTGTGTGTC
    CCCTCGCCCT GCCTGCACGG CGGCGCCTGC CGCGACCTCT TCGACGCCTT CGCCTGCGCC
    TGCGGCCCGG GCTGGGAGGG CCCGCGCTGC GAGGACCGCG CCGACCCGTG TCGCTCGGCG
    CCCTGCGCCC GCGGCCGCTG CCACGCACAC CCAGACGGTC ACTTCGAGTG CCGCTGCCCG
    CCTGGCTTCG CAGGCCCGCG CTGCAGGTTG CCTGTCCCAC CAGAGGGGTG CAGCCTGAAC
    ATCACCTGTC TCAACGGTGG CCAGTGTGAG GAGGGGCCCC AGGGGGCCAA CTGCAGCTGC
    CAGGAGAGCT TTGCTGGGCA GAGATGTCAG ATCCCCTGTG AAGCCAACCC CTGCTTGAAT
    GGGGGCACCT GCCGGGCAGC TGATGGGGTG TACGAATGTG TCTGCAATGC CAGGTTCTCA
    GGCCAGTTCT GCGAAGTGGT GAAAGGCCTA CCACTGCCGC TGCCGTTCCC GCTGCTGGAG
    GTAGCAGTGC CCGCGGCCTG CGCCTGCCTC CTCCTCCTCC TCTTGGGCCT CCTCTCAGGG
    ATCCTGGCGG CCAGAAAGCG CCGCCAGTCA GAGGGCACCT ACAGTCCGAG CCAGCAGGAG
    GTGGCCGGAG CCCGGCTGGA GATGGACAGC GTCCTCAAGG TGCCGCCCGA GGAGAGACTC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24312
    Clone ID Related Accession (Same CDS sequence) XM_015465485.1 , XM_003586670.4
    Accession Version XM_015465485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein crumbs homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG GGATGACTGC
    GGCGAGGATG TAGATGAGTG TGCCTCTCGG CCGTGCCTCA GTGGAGGCCT CTGCCAGGAC
    CTGCCCAATG GCTTCCACTG CCACTGTCCA GACGGCTACA CAGGCCCAAC GTGTCAGGAA
    GACATGGACG AATGCCTGTC GGAGCCTTGC CTCCATGGTG GGACCTGTGA AGACACCGTG
    GCAGGCTACA TCTGTGGGTG CCCCGAGGCC TGGGGTGGAT TGGATTGTTC TGTGCCACTC
    ACCGGCTGCC AGGGCCACAC TTGCCCACCG ACTGCCACCT GCGTCCCTAC CTTCGAGTCA
    GGGGTTCACA GTTACACCTG CCGCTGCCCA CCTGGTACCC ATGGACCTTT CTGTGGCCAG
    AATACTACAT TCTCCATGGT GGCTGGGAAC CCCTTACGGG CTTCGGTGCC AGCTGGCAGC
    TCCCTAGGTC TTGCCCTGAG GTTTCGTACC ACGATACGTG TGGGTGCCTT GGCCACGTGC
    TCTGACACTC AAGGCAGCGT GGAGCTGGCG CTGGTGGAGG CCAGGCTTCA GGCCACACTC
    TGGAGCCACG GCAAGAATGT GCTCGTCCTG AGGCTGCTGG AACCACCCCT CAATGATGGC
    CACTGGCACC GAGTGGAGGT GACGCTCCGC CTGGGGGTCC TGGAGTTGCG GCTCTGGCAT
    GAAGACTGTC CCGCCCACCT CTGTGTGGCC TCCAGTCCCA TGGCCACGGC TGCCCCGGCC
    CCCACGCCTG CTGGGTCCCG CTTCACCCAG CTGGGCGGCG TGGCCTTTGC GGGCTGCCTC
    CAGGATGTGC AGGTGGATGG GCATCTCCTG CTGCCCGAGG ACCTTGGTGA GAATGTCCTC
    CTGGGCTGCT GGCACCAGGA GCACTGCCAA CCTCCGCCTT GTGCCCACGG AGGGGTCTGC
    GTGGACCTGT GGACTCACTT CCATTGTGAC TGCCCCCGGC CCTACAGAGG TCCCACGTGT
    GCTGATGAGG TTCCTGCTGC CACCTTTGGC TTGGGGGGCA CCCTGAGCTC TGCCTCCTTC
    CTACTCGACC AGCCGCCAGG CCCCAACCTC ACCGTGTCCT TCCTCCTCCG CACCCGGGAA
    CCTGCTGGCC TTCTGCTCCA GCTCACCAAC GGTTCGGCCG CTGGCCTGAC AGTGTTCCTG
    AGCGAGGGCC AGATCTGGGC CGAGGCACTG GGCAGTCCTG CTCTGGTGCT CCCGGGGCGC
    TGGGATGACG GGCTCCGCCA CCTGGTGACA CTCAGCTTCG GGCCTGATCA GCTGTGGGGT
    GTGGGGCAGC AGGTGCACGT GGGCGGCAGG CTCCTCCCCG GTGACGCCGA GCCCTGGGGT
    GGGCCCTTCC GAGGCTGCAT GCAGGACGTG CGACTCAATG ACCTCCACCT CCCCTTCTTT
    CCACCACTGC TGGGGAACTC CAGCCTGCCC AGCGTGCCCG GCCGCGGGCA GTCCTGGAAC
    CTCACCATGG GCTGCATCTC TGAGGACACA TGCAATCCTG AGCCCTGTCT CAATGGTGGG
    ACTTGCCTCG TCACCTGGAA TGACTTCCAC TGCACCTGCC CTGCCAACTT CACTGGGCCC
    ACGTGTGCCC AGCAGCTGTG GTGTCCTGGC CAGCCCTGTC TCCCGCCTGC CACCTGTGAG
    GAGGTCCCTG ATGGCTTTGT CTGTGTGGCG GAGGCCACGT TCCGCGAGGG ACCCCCGGCG
    GAATTCAGCG GGCACAACGC GTCATCGGGC AGCGCTCTCA GCGGCCTCTC GCTGGTCTTC
    CGCACTCGCG ACCCCGAGGC AGGGCTGTTG CGCGCTGAGG ACGGCCCGGC CGCCGTGTGG
    CTGGCGGTGC GCAACGGCTC GCTGGCGGCC GGCGTGCGCA GCGGCGCCGG TTTGCCCCGC
    GCGGTGCTGC CAGCGCCCGG GCCGCGTGTG GCCGACGGCG CCTGGCACCG CGTGCGCCTG
    GCCATGGAGC AGCCCGCGGC CGTCGCTTCG CGCTGGCTGC TCTGGCTGGA CGGCGCGGCG
    ACGCCGGTGT CGCTGCGTGG CCTGGCCGGC GACCTGGACT TCCTGCGCGG CCCGGGCGCC
    GCGCGAGTCC TGCTGGCCGA GAACTTCACG GGCTGCCTGG GCCGCGTGGC GCTCGGCGGC
    CACCCGCTAC CCCTGGCGCG CCCGCGGCCC GGCGCGGCCC CCGGCTCCCG CGAGCCCTTC
    TCGGCCCGGC CCGGAGCGCC CGCCCCGCAC CTCGGCTGCA GCGGCGCTCC CGTGTGTGTC
    CCCTCGCCCT GCCTGCACGG CGGCGCCTGC CGCGACCTCT TCGACGCCTT CGCCTGCGCC
    TGCGGCCCGG GCTGGGAGGG CCCGCGCTGC GAGGACCGCG CCGACCCGTG TCGCTCGGCG
    CCCTGCGCCC GCGGCCGCTG CCACGCACAC CCAGACGGTC ACTTCGAGTG CCGCTGCCCG
    CCTGGCTTCG CAGGCCCGCG CTGCAGGTTG CCTGTCCCAC CAGAGGGGTG CAGCCTGAAC
    ATCACCTGTC TCAACGGTGG CCAGTGTGAG GAGGGGCCCC AGGGGGCCAA CTGCAGCTGC
    CAGGAGAGCT TTGCTGGGCA GAGATGTCAG ATCCCCTGTG AAGCCAACCC CTGCTTGAAT
    GGGGGCACCT GCCGGGCAGC TGATGGGGTG TACGAATGTG TCTGCAATGC CAGGTTCTCA
    GGCCAGTTCT GCGAAGTGGT GAAAGGCCTA CCACTGCCGC TGCCGTTCCC GCTGCTGGAG
    GTAGCAGTGC CCGCGGCCTG CGCCTGCCTC CTCCTCCTCC TCTTGGGCCT CCTCTCAGGG
    ATCCTGGCGG CCAGAAAGCG CCGCCAGTCA GAGGGCACCT ACAGTCCGAG CCAGCAGGAG
    GTGGCCGGAG CCCGGCTGGA GATGGACAGC GTCCTCAAGG TGCCGCCCGA GGAGAGACTC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320971.1
    CDS58..3903
    Translation

    Target ORF information:

    RefSeq Version XM_015465485.1
    Organism Bos taurus(cattle)
    Definition Bos taurus crumbs family member 2 (CRB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG GGATGACTGC
    GGCGAGGATG TAGATGAGTG TGCCTCTCGG CCGTGCCTCA GTGGAGGCCT CTGCCAGGAC
    CTGCCCAATG GCTTCCACTG CCACTGTCCA GACGGCTACA CAGGCCCAAC GTGTCAGGAA
    GACATGGACG AATGCCTGTC GGAGCCTTGC CTCCATGGTG GGACCTGTGA AGACACCGTG
    GCAGGCTACA TCTGTGGGTG CCCCGAGGCC TGGGGTGGAT TGGATTGTTC TGTGCCACTC
    ACCGGCTGCC AGGGCCACAC TTGCCCACCG ACTGCCACCT GCGTCCCTAC CTTCGAGTCA
    GGGGTTCACA GTTACACCTG CCGCTGCCCA CCTGGTACCC ATGGACCTTT CTGTGGCCAG
    AATACTACAT TCTCCATGGT GGCTGGGAAC CCCTTACGGG CTTCGGTGCC AGCTGGCAGC
    TCCCTAGGTC TTGCCCTGAG GTTTCGTACC ACGATACGTG TGGGTGCCTT GGCCACGTGC
    TCTGACACTC AAGGCAGCGT GGAGCTGGCG CTGGTGGAGG CCAGGCTTCA GGCCACACTC
    TGGAGCCACG GCAAGAATGT GCTCGTCCTG AGGCTGCTGG AACCACCCCT CAATGATGGC
    CACTGGCACC GAGTGGAGGT GACGCTCCGC CTGGGGGTCC TGGAGTTGCG GCTCTGGCAT
    GAAGACTGTC CCGCCCACCT CTGTGTGGCC TCCAGTCCCA TGGCCACGGC TGCCCCGGCC
    CCCACGCCTG CTGGGTCCCG CTTCACCCAG CTGGGCGGCG TGGCCTTTGC GGGCTGCCTC
    CAGGATGTGC AGGTGGATGG GCATCTCCTG CTGCCCGAGG ACCTTGGTGA GAATGTCCTC
    CTGGGCTGCT GGCACCAGGA GCACTGCCAA CCTCCGCCTT GTGCCCACGG AGGGGTCTGC
    GTGGACCTGT GGACTCACTT CCATTGTGAC TGCCCCCGGC CCTACAGAGG TCCCACGTGT
    GCTGATGAGG TTCCTGCTGC CACCTTTGGC TTGGGGGGCA CCCTGAGCTC TGCCTCCTTC
    CTACTCGACC AGCCGCCAGG CCCCAACCTC ACCGTGTCCT TCCTCCTCCG CACCCGGGAA
    CCTGCTGGCC TTCTGCTCCA GCTCACCAAC GGTTCGGCCG CTGGCCTGAC AGTGTTCCTG
    AGCGAGGGCC AGATCTGGGC CGAGGCACTG GGCAGTCCTG CTCTGGTGCT CCCGGGGCGC
    TGGGATGACG GGCTCCGCCA CCTGGTGACA CTCAGCTTCG GGCCTGATCA GCTGTGGGGT
    GTGGGGCAGC AGGTGCACGT GGGCGGCAGG CTCCTCCCCG GTGACGCCGA GCCCTGGGGT
    GGGCCCTTCC GAGGCTGCAT GCAGGACGTG CGACTCAATG ACCTCCACCT CCCCTTCTTT
    CCACCACTGC TGGGGAACTC CAGCCTGCCC AGCGTGCCCG GCCGCGGGCA GTCCTGGAAC
    CTCACCATGG GCTGCATCTC TGAGGACACA TGCAATCCTG AGCCCTGTCT CAATGGTGGG
    ACTTGCCTCG TCACCTGGAA TGACTTCCAC TGCACCTGCC CTGCCAACTT CACTGGGCCC
    ACGTGTGCCC AGCAGCTGTG GTGTCCTGGC CAGCCCTGTC TCCCGCCTGC CACCTGTGAG
    GAGGTCCCTG ATGGCTTTGT CTGTGTGGCG GAGGCCACGT TCCGCGAGGG ACCCCCGGCG
    GAATTCAGCG GGCACAACGC GTCATCGGGC AGCGCTCTCA GCGGCCTCTC GCTGGTCTTC
    CGCACTCGCG ACCCCGAGGC AGGGCTGTTG CGCGCTGAGG ACGGCCCGGC CGCCGTGTGG
    CTGGCGGTGC GCAACGGCTC GCTGGCGGCC GGCGTGCGCA GCGGCGCCGG TTTGCCCCGC
    GCGGTGCTGC CAGCGCCCGG GCCGCGTGTG GCCGACGGCG CCTGGCACCG CGTGCGCCTG
    GCCATGGAGC AGCCCGCGGC CGTCGCTTCG CGCTGGCTGC TCTGGCTGGA CGGCGCGGCG
    ACGCCGGTGT CGCTGCGTGG CCTGGCCGGC GACCTGGACT TCCTGCGCGG CCCGGGCGCC
    GCGCGAGTCC TGCTGGCCGA GAACTTCACG GGCTGCCTGG GCCGCGTGGC GCTCGGCGGC
    CACCCGCTAC CCCTGGCGCG CCCGCGGCCC GGCGCGGCCC CCGGCTCCCG CGAGCCCTTC
    TCGGCCCGGC CCGGAGCGCC CGCCCCGCAC CTCGGCTGCA GCGGCGCTCC CGTGTGTGTC
    CCCTCGCCCT GCCTGCACGG CGGCGCCTGC CGCGACCTCT TCGACGCCTT CGCCTGCGCC
    TGCGGCCCGG GCTGGGAGGG CCCGCGCTGC GAGGACCGCG CCGACCCGTG TCGCTCGGCG
    CCCTGCGCCC GCGGCCGCTG CCACGCACAC CCAGACGGTC ACTTCGAGTG CCGCTGCCCG
    CCTGGCTTCG CAGGCCCGCG CTGCAGGTTG CCTGTCCCAC CAGAGGGGTG CAGCCTGAAC
    ATCACCTGTC TCAACGGTGG CCAGTGTGAG GAGGGGCCCC AGGGGGCCAA CTGCAGCTGC
    CAGGAGAGCT TTGCTGGGCA GAGATGTCAG ATCCCCTGTG AAGCCAACCC CTGCTTGAAT
    GGGGGCACCT GCCGGGCAGC TGATGGGGTG TACGAATGTG TCTGCAATGC CAGGTTCTCA
    GGCCAGTTCT GCGAAGTGGT GAAAGGCCTA CCACTGCCGC TGCCGTTCCC GCTGCTGGAG
    GTAGCAGTGC CCGCGGCCTG CGCCTGCCTC CTCCTCCTCC TCTTGGGCCT CCTCTCAGGG
    ATCCTGGCGG CCAGAAAGCG CCGCCAGTCA GAGGGCACCT ACAGTCCGAG CCAGCAGGAG
    GTGGCCGGAG CCCGGCTGGA GATGGACAGC GTCCTCAAGG TGCCGCCCGA GGAGAGACTC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24312
    Clone ID Related Accession (Same CDS sequence) XM_015465485.1 , XM_003586670.4
    Accession Version XM_003586670.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein crumbs homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG GGATGACTGC
    GGCGAGGATG TAGATGAGTG TGCCTCTCGG CCGTGCCTCA GTGGAGGCCT CTGCCAGGAC
    CTGCCCAATG GCTTCCACTG CCACTGTCCA GACGGCTACA CAGGCCCAAC GTGTCAGGAA
    GACATGGACG AATGCCTGTC GGAGCCTTGC CTCCATGGTG GGACCTGTGA AGACACCGTG
    GCAGGCTACA TCTGTGGGTG CCCCGAGGCC TGGGGTGGAT TGGATTGTTC TGTGCCACTC
    ACCGGCTGCC AGGGCCACAC TTGCCCACCG ACTGCCACCT GCGTCCCTAC CTTCGAGTCA
    GGGGTTCACA GTTACACCTG CCGCTGCCCA CCTGGTACCC ATGGACCTTT CTGTGGCCAG
    AATACTACAT TCTCCATGGT GGCTGGGAAC CCCTTACGGG CTTCGGTGCC AGCTGGCAGC
    TCCCTAGGTC TTGCCCTGAG GTTTCGTACC ACGATACGTG TGGGTGCCTT GGCCACGTGC
    TCTGACACTC AAGGCAGCGT GGAGCTGGCG CTGGTGGAGG CCAGGCTTCA GGCCACACTC
    TGGAGCCACG GCAAGAATGT GCTCGTCCTG AGGCTGCTGG AACCACCCCT CAATGATGGC
    CACTGGCACC GAGTGGAGGT GACGCTCCGC CTGGGGGTCC TGGAGTTGCG GCTCTGGCAT
    GAAGACTGTC CCGCCCACCT CTGTGTGGCC TCCAGTCCCA TGGCCACGGC TGCCCCGGCC
    CCCACGCCTG CTGGGTCCCG CTTCACCCAG CTGGGCGGCG TGGCCTTTGC GGGCTGCCTC
    CAGGATGTGC AGGTGGATGG GCATCTCCTG CTGCCCGAGG ACCTTGGTGA GAATGTCCTC
    CTGGGCTGCT GGCACCAGGA GCACTGCCAA CCTCCGCCTT GTGCCCACGG AGGGGTCTGC
    GTGGACCTGT GGACTCACTT CCATTGTGAC TGCCCCCGGC CCTACAGAGG TCCCACGTGT
    GCTGATGAGG TTCCTGCTGC CACCTTTGGC TTGGGGGGCA CCCTGAGCTC TGCCTCCTTC
    CTACTCGACC AGCCGCCAGG CCCCAACCTC ACCGTGTCCT TCCTCCTCCG CACCCGGGAA
    CCTGCTGGCC TTCTGCTCCA GCTCACCAAC GGTTCGGCCG CTGGCCTGAC AGTGTTCCTG
    AGCGAGGGCC AGATCTGGGC CGAGGCACTG GGCAGTCCTG CTCTGGTGCT CCCGGGGCGC
    TGGGATGACG GGCTCCGCCA CCTGGTGACA CTCAGCTTCG GGCCTGATCA GCTGTGGGGT
    GTGGGGCAGC AGGTGCACGT GGGCGGCAGG CTCCTCCCCG GTGACGCCGA GCCCTGGGGT
    GGGCCCTTCC GAGGCTGCAT GCAGGACGTG CGACTCAATG ACCTCCACCT CCCCTTCTTT
    CCACCACTGC TGGGGAACTC CAGCCTGCCC AGCGTGCCCG GCCGCGGGCA GTCCTGGAAC
    CTCACCATGG GCTGCATCTC TGAGGACACA TGCAATCCTG AGCCCTGTCT CAATGGTGGG
    ACTTGCCTCG TCACCTGGAA TGACTTCCAC TGCACCTGCC CTGCCAACTT CACTGGGCCC
    ACGTGTGCCC AGCAGCTGTG GTGTCCTGGC CAGCCCTGTC TCCCGCCTGC CACCTGTGAG
    GAGGTCCCTG ATGGCTTTGT CTGTGTGGCG GAGGCCACGT TCCGCGAGGG ACCCCCGGCG
    GAATTCAGCG GGCACAACGC GTCATCGGGC AGCGCTCTCA GCGGCCTCTC GCTGGTCTTC
    CGCACTCGCG ACCCCGAGGC AGGGCTGTTG CGCGCTGAGG ACGGCCCGGC CGCCGTGTGG
    CTGGCGGTGC GCAACGGCTC GCTGGCGGCC GGCGTGCGCA GCGGCGCCGG TTTGCCCCGC
    GCGGTGCTGC CAGCGCCCGG GCCGCGTGTG GCCGACGGCG CCTGGCACCG CGTGCGCCTG
    GCCATGGAGC AGCCCGCGGC CGTCGCTTCG CGCTGGCTGC TCTGGCTGGA CGGCGCGGCG
    ACGCCGGTGT CGCTGCGTGG CCTGGCCGGC GACCTGGACT TCCTGCGCGG CCCGGGCGCC
    GCGCGAGTCC TGCTGGCCGA GAACTTCACG GGCTGCCTGG GCCGCGTGGC GCTCGGCGGC
    CACCCGCTAC CCCTGGCGCG CCCGCGGCCC GGCGCGGCCC CCGGCTCCCG CGAGCCCTTC
    TCGGCCCGGC CCGGAGCGCC CGCCCCGCAC CTCGGCTGCA GCGGCGCTCC CGTGTGTGTC
    CCCTCGCCCT GCCTGCACGG CGGCGCCTGC CGCGACCTCT TCGACGCCTT CGCCTGCGCC
    TGCGGCCCGG GCTGGGAGGG CCCGCGCTGC GAGGACCGCG CCGACCCGTG TCGCTCGGCG
    CCCTGCGCCC GCGGCCGCTG CCACGCACAC CCAGACGGTC ACTTCGAGTG CCGCTGCCCG
    CCTGGCTTCG CAGGCCCGCG CTGCAGGTTG CCTGTCCCAC CAGAGGGGTG CAGCCTGAAC
    ATCACCTGTC TCAACGGTGG CCAGTGTGAG GAGGGGCCCC AGGGGGCCAA CTGCAGCTGC
    CAGGAGAGCT TTGCTGGGCA GAGATGTCAG ATCCCCTGTG AAGCCAACCC CTGCTTGAAT
    GGGGGCACCT GCCGGGCAGC TGATGGGGTG TACGAATGTG TCTGCAATGC CAGGTTCTCA
    GGCCAGTTCT GCGAAGTGGT GAAAGGCCTA CCACTGCCGC TGCCGTTCCC GCTGCTGGAG
    GTAGCAGTGC CCGCGGCCTG CGCCTGCCTC CTCCTCCTCC TCTTGGGCCT CCTCTCAGGG
    ATCCTGGCGG CCAGAAAGCG CCGCCAGTCA GAGGGCACCT ACAGTCCGAG CCAGCAGGAG
    GTGGCCGGAG CCCGGCTGGA GATGGACAGC GTCCTCAAGG TGCCGCCCGA GGAGAGACTC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586718.1
    CDS58..3903
    Translation

    Target ORF information:

    RefSeq Version XM_003586670.4
    Organism Bos taurus(cattle)
    Definition Bos taurus crumbs family member 2 (CRB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586670.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG GGATGACTGC
    GGCGAGGATG TAGATGAGTG TGCCTCTCGG CCGTGCCTCA GTGGAGGCCT CTGCCAGGAC
    CTGCCCAATG GCTTCCACTG CCACTGTCCA GACGGCTACA CAGGCCCAAC GTGTCAGGAA
    GACATGGACG AATGCCTGTC GGAGCCTTGC CTCCATGGTG GGACCTGTGA AGACACCGTG
    GCAGGCTACA TCTGTGGGTG CCCCGAGGCC TGGGGTGGAT TGGATTGTTC TGTGCCACTC
    ACCGGCTGCC AGGGCCACAC TTGCCCACCG ACTGCCACCT GCGTCCCTAC CTTCGAGTCA
    GGGGTTCACA GTTACACCTG CCGCTGCCCA CCTGGTACCC ATGGACCTTT CTGTGGCCAG
    AATACTACAT TCTCCATGGT GGCTGGGAAC CCCTTACGGG CTTCGGTGCC AGCTGGCAGC
    TCCCTAGGTC TTGCCCTGAG GTTTCGTACC ACGATACGTG TGGGTGCCTT GGCCACGTGC
    TCTGACACTC AAGGCAGCGT GGAGCTGGCG CTGGTGGAGG CCAGGCTTCA GGCCACACTC
    TGGAGCCACG GCAAGAATGT GCTCGTCCTG AGGCTGCTGG AACCACCCCT CAATGATGGC
    CACTGGCACC GAGTGGAGGT GACGCTCCGC CTGGGGGTCC TGGAGTTGCG GCTCTGGCAT
    GAAGACTGTC CCGCCCACCT CTGTGTGGCC TCCAGTCCCA TGGCCACGGC TGCCCCGGCC
    CCCACGCCTG CTGGGTCCCG CTTCACCCAG CTGGGCGGCG TGGCCTTTGC GGGCTGCCTC
    CAGGATGTGC AGGTGGATGG GCATCTCCTG CTGCCCGAGG ACCTTGGTGA GAATGTCCTC
    CTGGGCTGCT GGCACCAGGA GCACTGCCAA CCTCCGCCTT GTGCCCACGG AGGGGTCTGC
    GTGGACCTGT GGACTCACTT CCATTGTGAC TGCCCCCGGC CCTACAGAGG TCCCACGTGT
    GCTGATGAGG TTCCTGCTGC CACCTTTGGC TTGGGGGGCA CCCTGAGCTC TGCCTCCTTC
    CTACTCGACC AGCCGCCAGG CCCCAACCTC ACCGTGTCCT TCCTCCTCCG CACCCGGGAA
    CCTGCTGGCC TTCTGCTCCA GCTCACCAAC GGTTCGGCCG CTGGCCTGAC AGTGTTCCTG
    AGCGAGGGCC AGATCTGGGC CGAGGCACTG GGCAGTCCTG CTCTGGTGCT CCCGGGGCGC
    TGGGATGACG GGCTCCGCCA CCTGGTGACA CTCAGCTTCG GGCCTGATCA GCTGTGGGGT
    GTGGGGCAGC AGGTGCACGT GGGCGGCAGG CTCCTCCCCG GTGACGCCGA GCCCTGGGGT
    GGGCCCTTCC GAGGCTGCAT GCAGGACGTG CGACTCAATG ACCTCCACCT CCCCTTCTTT
    CCACCACTGC TGGGGAACTC CAGCCTGCCC AGCGTGCCCG GCCGCGGGCA GTCCTGGAAC
    CTCACCATGG GCTGCATCTC TGAGGACACA TGCAATCCTG AGCCCTGTCT CAATGGTGGG
    ACTTGCCTCG TCACCTGGAA TGACTTCCAC TGCACCTGCC CTGCCAACTT CACTGGGCCC
    ACGTGTGCCC AGCAGCTGTG GTGTCCTGGC CAGCCCTGTC TCCCGCCTGC CACCTGTGAG
    GAGGTCCCTG ATGGCTTTGT CTGTGTGGCG GAGGCCACGT TCCGCGAGGG ACCCCCGGCG
    GAATTCAGCG GGCACAACGC GTCATCGGGC AGCGCTCTCA GCGGCCTCTC GCTGGTCTTC
    CGCACTCGCG ACCCCGAGGC AGGGCTGTTG CGCGCTGAGG ACGGCCCGGC CGCCGTGTGG
    CTGGCGGTGC GCAACGGCTC GCTGGCGGCC GGCGTGCGCA GCGGCGCCGG TTTGCCCCGC
    GCGGTGCTGC CAGCGCCCGG GCCGCGTGTG GCCGACGGCG CCTGGCACCG CGTGCGCCTG
    GCCATGGAGC AGCCCGCGGC CGTCGCTTCG CGCTGGCTGC TCTGGCTGGA CGGCGCGGCG
    ACGCCGGTGT CGCTGCGTGG CCTGGCCGGC GACCTGGACT TCCTGCGCGG CCCGGGCGCC
    GCGCGAGTCC TGCTGGCCGA GAACTTCACG GGCTGCCTGG GCCGCGTGGC GCTCGGCGGC
    CACCCGCTAC CCCTGGCGCG CCCGCGGCCC GGCGCGGCCC CCGGCTCCCG CGAGCCCTTC
    TCGGCCCGGC CCGGAGCGCC CGCCCCGCAC CTCGGCTGCA GCGGCGCTCC CGTGTGTGTC
    CCCTCGCCCT GCCTGCACGG CGGCGCCTGC CGCGACCTCT TCGACGCCTT CGCCTGCGCC
    TGCGGCCCGG GCTGGGAGGG CCCGCGCTGC GAGGACCGCG CCGACCCGTG TCGCTCGGCG
    CCCTGCGCCC GCGGCCGCTG CCACGCACAC CCAGACGGTC ACTTCGAGTG CCGCTGCCCG
    CCTGGCTTCG CAGGCCCGCG CTGCAGGTTG CCTGTCCCAC CAGAGGGGTG CAGCCTGAAC
    ATCACCTGTC TCAACGGTGG CCAGTGTGAG GAGGGGCCCC AGGGGGCCAA CTGCAGCTGC
    CAGGAGAGCT TTGCTGGGCA GAGATGTCAG ATCCCCTGTG AAGCCAACCC CTGCTTGAAT
    GGGGGCACCT GCCGGGCAGC TGATGGGGTG TACGAATGTG TCTGCAATGC CAGGTTCTCA
    GGCCAGTTCT GCGAAGTGGT GAAAGGCCTA CCACTGCCGC TGCCGTTCCC GCTGCTGGAG
    GTAGCAGTGC CCGCGGCCTG CGCCTGCCTC CTCCTCCTCC TCTTGGGCCT CCTCTCAGGG
    ATCCTGGCGG CCAGAAAGCG CCGCCAGTCA GAGGGCACCT ACAGTCCGAG CCAGCAGGAG
    GTGGCCGGAG CCCGGCTGGA GATGGACAGC GTCCTCAAGG TGCCGCCCGA GGAGAGACTC
    ATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24313
    Clone ID Related Accession (Same CDS sequence) XM_005213164.3 , XM_015465486.1
    Accession Version XM_005213164.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein crumbs homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104301.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG CCCAACGTGT
    CAGGAAGACA TGGACGAATG CCTGTCGGAG CCTTGCCTCC ATGGTGGGAC CTGTGAAGAC
    ACCGTGGCAG GCTACATCTG TGGGTGCCCC GAGGCCTGGG GTGGATTGGA TTGTTCTGTG
    CCACTCACCG GCTGCCAGGG CCACACTTGC CCACCGACTG CCACCTGCGT CCCTACCTTC
    GAGTCAGGGG TTCACAGTTA CACCTGCCGC TGCCCACCTG GTACCCATGG ACCTTTCTGT
    GGCCAGAATA CTACATTCTC CATGGTGGCT GGGAACCCCT TACGGGCTTC GGTGCCAGCT
    GGCAGCTCCC TAGGTCTTGC CCTGAGGTTT CGTACCACGA TACGTGTGGG TGCCTTGGCC
    ACGTGCTCTG ACACTCAAGG CAGCGTGGAG CTGGCGCTGG TGGAGGCCAG GCTTCAGGCC
    ACACTCTGGA GCCACGGCAA GAATGTGCTC GTCCTGAGGC TGCTGGAACC ACCCCTCAAT
    GATGGCCACT GGCACCGAGT GGAGGTGACG CTCCGCCTGG GGGTCCTGGA GTTGCGGCTC
    TGGCATGAAG ACTGTCCCGC CCACCTCTGT GTGGCCTCCA GTCCCATGGC CACGGCTGCC
    CCGGCCCCCA CGCCTGCTGG GTCCCGCTTC ACCCAGCTGG GCGGCGTGGC CTTTGCGGGC
    TGCCTCCAGG ATGTGCAGGT GGATGGGCAT CTCCTGCTGC CCGAGGACCT TGGTGAGAAT
    GTCCTCCTGG GCTGCTGGCA CCAGGAGCAC TGCCAACCTC CGCCTTGTGC CCACGGAGGG
    GTCTGCGTGG ACCTGTGGAC TCACTTCCAT TGTGACTGCC CCCGGCCCTA CAGAGGTCCC
    ACGTGTGCTG ATGAGGTTCC TGCTGCCACC TTTGGCTTGG GGGGCACCCT GAGCTCTGCC
    TCCTTCCTAC TCGACCAGCC GCCAGGCCCC AACCTCACCG TGTCCTTCCT CCTCCGCACC
    CGGGAACCTG CTGGCCTTCT GCTCCAGCTC ACCAACGGTT CGGCCGCTGG CCTGACAGTG
    TTCCTGAGCG AGGGCCAGAT CTGGGCCGAG GCACTGGGCA GTCCTGCTCT GGTGCTCCCG
    GGGCGCTGGG ATGACGGGCT CCGCCACCTG GTGACACTCA GCTTCGGGCC TGATCAGCTG
    TGGGGTGTGG GGCAGCAGGT GCACGTGGGC GGCAGGCTCC TCCCCGGTGA CGCCGAGCCC
    TGGGGTGGGC CCTTCCGAGG CTGCATGCAG GACGTGCGAC TCAATGACCT CCACCTCCCC
    TTCTTTCCAC CACTGCTGGG GAACTCCAGC CTGCCCAGCG TGCCCGGCCG CGGGCAGTCC
    TGGAACCTCA CCATGGGCTG CATCTCTGAG GACACATGCA ATCCTGAGCC CTGTCTCAAT
    GGTGGGACTT GCCTCGTCAC CTGGAATGAC TTCCACTGCA CCTGCCCTGC CAACTTCACT
    GGGCCCACGT GTGCCCAGCA GCTGTGGTGT CCTGGCCAGC CCTGTCTCCC GCCTGCCACC
    TGTGAGGAGG TCCCTGATGG CTTTGTCTGT GTGGCGGAGG CCACGTTCCG CGAGGGACCC
    CCGGCGGAAT TCAGCGGGCA CAACGCGTCA TCGGGCAGCG CTCTCAGCGG CCTCTCGCTG
    GTCTTCCGCA CTCGCGACCC CGAGGCAGGG CTGTTGCGCG CTGAGGACGG CCCGGCCGCC
    GTGTGGCTGG CGGTGCGCAA CGGCTCGCTG GCGGCCGGCG TGCGCAGCGG CGCCGGTTTG
    CCCCGCGCGG TGCTGCCAGC GCCCGGGCCG CGTGTGGCCG ACGGCGCCTG GCACCGCGTG
    CGCCTGGCCA TGGAGCAGCC CGCGGCCGTC GCTTCGCGCT GGCTGCTCTG GCTGGACGGC
    GCGGCGACGC CGGTGTCGCT GCGTGGCCTG GCCGGCGACC TGGACTTCCT GCGCGGCCCG
    GGCGCCGCGC GAGTCCTGCT GGCCGAGAAC TTCACGGGCT GCCTGGGCCG CGTGGCGCTC
    GGCGGCCACC CGCTACCCCT GGCGCGCCCG CGGCCCGGCG CGGCCCCCGG CTCCCGCGAG
    CCCTTCTCGG CCCGGCCCGG AGCGCCCGCC CCGCACCTCG GCTGCAGCGG CGCTCCCGTG
    TGTGTCCCCT CGCCCTGCCT GCACGGCGGC GCCTGCCGCG ACCTCTTCGA CGCCTTCGCC
    TGCGCCTGCG GCCCGGGCTG GGAGGGCCCG CGCTGCGAGG ACCGCGCCGA CCCGTGTCGC
    TCGGCGCCCT GCGCCCGCGG CCGCTGCCAC GCACACCCAG ACGGTCACTT CGAGTGCCGC
    TGCCCGCCTG GCTTCGCAGG CCCGCGCTGC AGGTTGCCTG TCCCACCAGA GGGGTGCAGC
    CTGAACATCA CCTGTCTCAA CGGTGGCCAG TGTGAGGAGG GGCCCCAGGG GGCCAACTGC
    AGCTGCCAGG AGAGCTTTGC TGGGCAGAGA TGTCAGATCC CCTGTGAAGC CAACCCCTGC
    TTGAATGGGG GCACCTGCCG GGCAGCTGAT GGGGTGTACG AATGTGTCTG CAATGCCAGG
    TTCTCAGGCC AGTTCTGCGA AGTGGTGAAA GGCCTACCAC TGCCGCTGCC GTTCCCGCTG
    CTGGAGGTAG CAGTGCCCGC GGCCTGCGCC TGCCTCCTCC TCCTCCTCTT GGGCCTCCTC
    TCAGGGATCC TGGCGGCCAG AAAGCGCCGC CAGTCAGAGG GCACCTACAG TCCGAGCCAG
    CAGGAGGTGG CCGGAGCCCG GCTGGAGATG GACAGCGTCC TCAAGGTGCC GCCCGAGGAG
    AGACTCATCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213221.1
    CDS58..3789
    Translation

    Target ORF information:

    RefSeq Version XM_005213164.3
    Organism Bos taurus(cattle)
    Definition Bos taurus crumbs family member 2 (CRB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213164.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG CCCAACGTGT
    CAGGAAGACA TGGACGAATG CCTGTCGGAG CCTTGCCTCC ATGGTGGGAC CTGTGAAGAC
    ACCGTGGCAG GCTACATCTG TGGGTGCCCC GAGGCCTGGG GTGGATTGGA TTGTTCTGTG
    CCACTCACCG GCTGCCAGGG CCACACTTGC CCACCGACTG CCACCTGCGT CCCTACCTTC
    GAGTCAGGGG TTCACAGTTA CACCTGCCGC TGCCCACCTG GTACCCATGG ACCTTTCTGT
    GGCCAGAATA CTACATTCTC CATGGTGGCT GGGAACCCCT TACGGGCTTC GGTGCCAGCT
    GGCAGCTCCC TAGGTCTTGC CCTGAGGTTT CGTACCACGA TACGTGTGGG TGCCTTGGCC
    ACGTGCTCTG ACACTCAAGG CAGCGTGGAG CTGGCGCTGG TGGAGGCCAG GCTTCAGGCC
    ACACTCTGGA GCCACGGCAA GAATGTGCTC GTCCTGAGGC TGCTGGAACC ACCCCTCAAT
    GATGGCCACT GGCACCGAGT GGAGGTGACG CTCCGCCTGG GGGTCCTGGA GTTGCGGCTC
    TGGCATGAAG ACTGTCCCGC CCACCTCTGT GTGGCCTCCA GTCCCATGGC CACGGCTGCC
    CCGGCCCCCA CGCCTGCTGG GTCCCGCTTC ACCCAGCTGG GCGGCGTGGC CTTTGCGGGC
    TGCCTCCAGG ATGTGCAGGT GGATGGGCAT CTCCTGCTGC CCGAGGACCT TGGTGAGAAT
    GTCCTCCTGG GCTGCTGGCA CCAGGAGCAC TGCCAACCTC CGCCTTGTGC CCACGGAGGG
    GTCTGCGTGG ACCTGTGGAC TCACTTCCAT TGTGACTGCC CCCGGCCCTA CAGAGGTCCC
    ACGTGTGCTG ATGAGGTTCC TGCTGCCACC TTTGGCTTGG GGGGCACCCT GAGCTCTGCC
    TCCTTCCTAC TCGACCAGCC GCCAGGCCCC AACCTCACCG TGTCCTTCCT CCTCCGCACC
    CGGGAACCTG CTGGCCTTCT GCTCCAGCTC ACCAACGGTT CGGCCGCTGG CCTGACAGTG
    TTCCTGAGCG AGGGCCAGAT CTGGGCCGAG GCACTGGGCA GTCCTGCTCT GGTGCTCCCG
    GGGCGCTGGG ATGACGGGCT CCGCCACCTG GTGACACTCA GCTTCGGGCC TGATCAGCTG
    TGGGGTGTGG GGCAGCAGGT GCACGTGGGC GGCAGGCTCC TCCCCGGTGA CGCCGAGCCC
    TGGGGTGGGC CCTTCCGAGG CTGCATGCAG GACGTGCGAC TCAATGACCT CCACCTCCCC
    TTCTTTCCAC CACTGCTGGG GAACTCCAGC CTGCCCAGCG TGCCCGGCCG CGGGCAGTCC
    TGGAACCTCA CCATGGGCTG CATCTCTGAG GACACATGCA ATCCTGAGCC CTGTCTCAAT
    GGTGGGACTT GCCTCGTCAC CTGGAATGAC TTCCACTGCA CCTGCCCTGC CAACTTCACT
    GGGCCCACGT GTGCCCAGCA GCTGTGGTGT CCTGGCCAGC CCTGTCTCCC GCCTGCCACC
    TGTGAGGAGG TCCCTGATGG CTTTGTCTGT GTGGCGGAGG CCACGTTCCG CGAGGGACCC
    CCGGCGGAAT TCAGCGGGCA CAACGCGTCA TCGGGCAGCG CTCTCAGCGG CCTCTCGCTG
    GTCTTCCGCA CTCGCGACCC CGAGGCAGGG CTGTTGCGCG CTGAGGACGG CCCGGCCGCC
    GTGTGGCTGG CGGTGCGCAA CGGCTCGCTG GCGGCCGGCG TGCGCAGCGG CGCCGGTTTG
    CCCCGCGCGG TGCTGCCAGC GCCCGGGCCG CGTGTGGCCG ACGGCGCCTG GCACCGCGTG
    CGCCTGGCCA TGGAGCAGCC CGCGGCCGTC GCTTCGCGCT GGCTGCTCTG GCTGGACGGC
    GCGGCGACGC CGGTGTCGCT GCGTGGCCTG GCCGGCGACC TGGACTTCCT GCGCGGCCCG
    GGCGCCGCGC GAGTCCTGCT GGCCGAGAAC TTCACGGGCT GCCTGGGCCG CGTGGCGCTC
    GGCGGCCACC CGCTACCCCT GGCGCGCCCG CGGCCCGGCG CGGCCCCCGG CTCCCGCGAG
    CCCTTCTCGG CCCGGCCCGG AGCGCCCGCC CCGCACCTCG GCTGCAGCGG CGCTCCCGTG
    TGTGTCCCCT CGCCCTGCCT GCACGGCGGC GCCTGCCGCG ACCTCTTCGA CGCCTTCGCC
    TGCGCCTGCG GCCCGGGCTG GGAGGGCCCG CGCTGCGAGG ACCGCGCCGA CCCGTGTCGC
    TCGGCGCCCT GCGCCCGCGG CCGCTGCCAC GCACACCCAG ACGGTCACTT CGAGTGCCGC
    TGCCCGCCTG GCTTCGCAGG CCCGCGCTGC AGGTTGCCTG TCCCACCAGA GGGGTGCAGC
    CTGAACATCA CCTGTCTCAA CGGTGGCCAG TGTGAGGAGG GGCCCCAGGG GGCCAACTGC
    AGCTGCCAGG AGAGCTTTGC TGGGCAGAGA TGTCAGATCC CCTGTGAAGC CAACCCCTGC
    TTGAATGGGG GCACCTGCCG GGCAGCTGAT GGGGTGTACG AATGTGTCTG CAATGCCAGG
    TTCTCAGGCC AGTTCTGCGA AGTGGTGAAA GGCCTACCAC TGCCGCTGCC GTTCCCGCTG
    CTGGAGGTAG CAGTGCCCGC GGCCTGCGCC TGCCTCCTCC TCCTCCTCTT GGGCCTCCTC
    TCAGGGATCC TGGCGGCCAG AAAGCGCCGC CAGTCAGAGG GCACCTACAG TCCGAGCCAG
    CAGGAGGTGG CCGGAGCCCG GCTGGAGATG GACAGCGTCC TCAAGGTGCC GCCCGAGGAG
    AGACTCATCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24313
    Clone ID Related Accession (Same CDS sequence) XM_005213164.3 , XM_015465486.1
    Accession Version XM_015465486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein crumbs homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645921.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG CCCAACGTGT
    CAGGAAGACA TGGACGAATG CCTGTCGGAG CCTTGCCTCC ATGGTGGGAC CTGTGAAGAC
    ACCGTGGCAG GCTACATCTG TGGGTGCCCC GAGGCCTGGG GTGGATTGGA TTGTTCTGTG
    CCACTCACCG GCTGCCAGGG CCACACTTGC CCACCGACTG CCACCTGCGT CCCTACCTTC
    GAGTCAGGGG TTCACAGTTA CACCTGCCGC TGCCCACCTG GTACCCATGG ACCTTTCTGT
    GGCCAGAATA CTACATTCTC CATGGTGGCT GGGAACCCCT TACGGGCTTC GGTGCCAGCT
    GGCAGCTCCC TAGGTCTTGC CCTGAGGTTT CGTACCACGA TACGTGTGGG TGCCTTGGCC
    ACGTGCTCTG ACACTCAAGG CAGCGTGGAG CTGGCGCTGG TGGAGGCCAG GCTTCAGGCC
    ACACTCTGGA GCCACGGCAA GAATGTGCTC GTCCTGAGGC TGCTGGAACC ACCCCTCAAT
    GATGGCCACT GGCACCGAGT GGAGGTGACG CTCCGCCTGG GGGTCCTGGA GTTGCGGCTC
    TGGCATGAAG ACTGTCCCGC CCACCTCTGT GTGGCCTCCA GTCCCATGGC CACGGCTGCC
    CCGGCCCCCA CGCCTGCTGG GTCCCGCTTC ACCCAGCTGG GCGGCGTGGC CTTTGCGGGC
    TGCCTCCAGG ATGTGCAGGT GGATGGGCAT CTCCTGCTGC CCGAGGACCT TGGTGAGAAT
    GTCCTCCTGG GCTGCTGGCA CCAGGAGCAC TGCCAACCTC CGCCTTGTGC CCACGGAGGG
    GTCTGCGTGG ACCTGTGGAC TCACTTCCAT TGTGACTGCC CCCGGCCCTA CAGAGGTCCC
    ACGTGTGCTG ATGAGGTTCC TGCTGCCACC TTTGGCTTGG GGGGCACCCT GAGCTCTGCC
    TCCTTCCTAC TCGACCAGCC GCCAGGCCCC AACCTCACCG TGTCCTTCCT CCTCCGCACC
    CGGGAACCTG CTGGCCTTCT GCTCCAGCTC ACCAACGGTT CGGCCGCTGG CCTGACAGTG
    TTCCTGAGCG AGGGCCAGAT CTGGGCCGAG GCACTGGGCA GTCCTGCTCT GGTGCTCCCG
    GGGCGCTGGG ATGACGGGCT CCGCCACCTG GTGACACTCA GCTTCGGGCC TGATCAGCTG
    TGGGGTGTGG GGCAGCAGGT GCACGTGGGC GGCAGGCTCC TCCCCGGTGA CGCCGAGCCC
    TGGGGTGGGC CCTTCCGAGG CTGCATGCAG GACGTGCGAC TCAATGACCT CCACCTCCCC
    TTCTTTCCAC CACTGCTGGG GAACTCCAGC CTGCCCAGCG TGCCCGGCCG CGGGCAGTCC
    TGGAACCTCA CCATGGGCTG CATCTCTGAG GACACATGCA ATCCTGAGCC CTGTCTCAAT
    GGTGGGACTT GCCTCGTCAC CTGGAATGAC TTCCACTGCA CCTGCCCTGC CAACTTCACT
    GGGCCCACGT GTGCCCAGCA GCTGTGGTGT CCTGGCCAGC CCTGTCTCCC GCCTGCCACC
    TGTGAGGAGG TCCCTGATGG CTTTGTCTGT GTGGCGGAGG CCACGTTCCG CGAGGGACCC
    CCGGCGGAAT TCAGCGGGCA CAACGCGTCA TCGGGCAGCG CTCTCAGCGG CCTCTCGCTG
    GTCTTCCGCA CTCGCGACCC CGAGGCAGGG CTGTTGCGCG CTGAGGACGG CCCGGCCGCC
    GTGTGGCTGG CGGTGCGCAA CGGCTCGCTG GCGGCCGGCG TGCGCAGCGG CGCCGGTTTG
    CCCCGCGCGG TGCTGCCAGC GCCCGGGCCG CGTGTGGCCG ACGGCGCCTG GCACCGCGTG
    CGCCTGGCCA TGGAGCAGCC CGCGGCCGTC GCTTCGCGCT GGCTGCTCTG GCTGGACGGC
    GCGGCGACGC CGGTGTCGCT GCGTGGCCTG GCCGGCGACC TGGACTTCCT GCGCGGCCCG
    GGCGCCGCGC GAGTCCTGCT GGCCGAGAAC TTCACGGGCT GCCTGGGCCG CGTGGCGCTC
    GGCGGCCACC CGCTACCCCT GGCGCGCCCG CGGCCCGGCG CGGCCCCCGG CTCCCGCGAG
    CCCTTCTCGG CCCGGCCCGG AGCGCCCGCC CCGCACCTCG GCTGCAGCGG CGCTCCCGTG
    TGTGTCCCCT CGCCCTGCCT GCACGGCGGC GCCTGCCGCG ACCTCTTCGA CGCCTTCGCC
    TGCGCCTGCG GCCCGGGCTG GGAGGGCCCG CGCTGCGAGG ACCGCGCCGA CCCGTGTCGC
    TCGGCGCCCT GCGCCCGCGG CCGCTGCCAC GCACACCCAG ACGGTCACTT CGAGTGCCGC
    TGCCCGCCTG GCTTCGCAGG CCCGCGCTGC AGGTTGCCTG TCCCACCAGA GGGGTGCAGC
    CTGAACATCA CCTGTCTCAA CGGTGGCCAG TGTGAGGAGG GGCCCCAGGG GGCCAACTGC
    AGCTGCCAGG AGAGCTTTGC TGGGCAGAGA TGTCAGATCC CCTGTGAAGC CAACCCCTGC
    TTGAATGGGG GCACCTGCCG GGCAGCTGAT GGGGTGTACG AATGTGTCTG CAATGCCAGG
    TTCTCAGGCC AGTTCTGCGA AGTGGTGAAA GGCCTACCAC TGCCGCTGCC GTTCCCGCTG
    CTGGAGGTAG CAGTGCCCGC GGCCTGCGCC TGCCTCCTCC TCCTCCTCTT GGGCCTCCTC
    TCAGGGATCC TGGCGGCCAG AAAGCGCCGC CAGTCAGAGG GCACCTACAG TCCGAGCCAG
    CAGGAGGTGG CCGGAGCCCG GCTGGAGATG GACAGCGTCC TCAAGGTGCC GCCCGAGGAG
    AGACTCATCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320972.1
    CDS58..3789
    Translation

    Target ORF information:

    RefSeq Version XM_015465486.1
    Organism Bos taurus(cattle)
    Definition Bos taurus crumbs family member 2 (CRB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGCTGG CCTGGCCTGG GACCCGGGAC CCCAGGCCCT CGGCCCATCT CCTGCTGCTG 
    CTGCTGCTGC TGCACAGGGC CCCAGTCCTC GCTCTACCGG CTGGGACGGT GCCTTCAGAG
    ACCCCGGGTG CCTGCGCCTC AGACCCATGC GCTCCAGGGA CCAAGTGCCA GGCTACAGAG
    AACGCTGGCT ACAGCTGCGT GCCCCTGGAG CCCCGGGGCT GTGCCAGTCA GCCATGCCAC
    CACGGCGCTC TGTGTGTGCC CCAGGGCCCA GGGCCCGATG ACTTCCGCTG CTACTGTGTG
    CCGGGATTCC AGGGTCCACA CTGTGAGCTG GACATCGATG AGTGTGCGTC CCGGCCCTGC
    CACCATGGGG CCACCTGCCG CAACCTGGCG GATCGTTACG AGTGCCACTG CCCGCTCGGC
    TATGAAGGCG TGACCTGCGA GACGGAGGTG GACGAGTGCG CCTCGGCGCC CTGCCTGCAC
    GGGGGCTCGT GCCTGGACGG CGTGGGCTCA TACCGCTGCG TGTGCGCGCC GGGCTACGGG
    GGCGCCAGCT GCCAGCTGGA CCTGGACGAG TGCCAGAGCC AGCCGTGCGC ACACGGGGGC
    GTGTGCCGCG ACCTGGTCAA CGGGTTCCGG TGCGACTGCG CGGACACGGG CTACGAGGGC
    GAGCGCTGCG AGCTGGAGGT TCTGGAGTGC GCGTCGGCGC CCTGCGCGAA CAACGCGTCC
    TGCCTCGAGG GCCTCGGGAG CTTCCGCTGC CTCTGCTGGC CAGGCTACAG TGGCGAGCGG
    TGTGAGGTGG ATGAGGACGA ATGTGCGGTG GGCCCCTGCC AGCATGGGGG CCAGTGCCTG
    CAGCGCTCGG ATCCAACCCT CTATGGGGGC ATCCAGGCCA CTTTCCCAGA TGACTTCAGC
    TTTCGCCAGG CTGCTGGCTT CGTGTGCCGC TGCCCTCCGG GCTTTGAGGG CCCAACGTGT
    CAGGAAGACA TGGACGAATG CCTGTCGGAG CCTTGCCTCC ATGGTGGGAC CTGTGAAGAC
    ACCGTGGCAG GCTACATCTG TGGGTGCCCC GAGGCCTGGG GTGGATTGGA TTGTTCTGTG
    CCACTCACCG GCTGCCAGGG CCACACTTGC CCACCGACTG CCACCTGCGT CCCTACCTTC
    GAGTCAGGGG TTCACAGTTA CACCTGCCGC TGCCCACCTG GTACCCATGG ACCTTTCTGT
    GGCCAGAATA CTACATTCTC CATGGTGGCT GGGAACCCCT TACGGGCTTC GGTGCCAGCT
    GGCAGCTCCC TAGGTCTTGC CCTGAGGTTT CGTACCACGA TACGTGTGGG TGCCTTGGCC
    ACGTGCTCTG ACACTCAAGG CAGCGTGGAG CTGGCGCTGG TGGAGGCCAG GCTTCAGGCC
    ACACTCTGGA GCCACGGCAA GAATGTGCTC GTCCTGAGGC TGCTGGAACC ACCCCTCAAT
    GATGGCCACT GGCACCGAGT GGAGGTGACG CTCCGCCTGG GGGTCCTGGA GTTGCGGCTC
    TGGCATGAAG ACTGTCCCGC CCACCTCTGT GTGGCCTCCA GTCCCATGGC CACGGCTGCC
    CCGGCCCCCA CGCCTGCTGG GTCCCGCTTC ACCCAGCTGG GCGGCGTGGC CTTTGCGGGC
    TGCCTCCAGG ATGTGCAGGT GGATGGGCAT CTCCTGCTGC CCGAGGACCT TGGTGAGAAT
    GTCCTCCTGG GCTGCTGGCA CCAGGAGCAC TGCCAACCTC CGCCTTGTGC CCACGGAGGG
    GTCTGCGTGG ACCTGTGGAC TCACTTCCAT TGTGACTGCC CCCGGCCCTA CAGAGGTCCC
    ACGTGTGCTG ATGAGGTTCC TGCTGCCACC TTTGGCTTGG GGGGCACCCT GAGCTCTGCC
    TCCTTCCTAC TCGACCAGCC GCCAGGCCCC AACCTCACCG TGTCCTTCCT CCTCCGCACC
    CGGGAACCTG CTGGCCTTCT GCTCCAGCTC ACCAACGGTT CGGCCGCTGG CCTGACAGTG
    TTCCTGAGCG AGGGCCAGAT CTGGGCCGAG GCACTGGGCA GTCCTGCTCT GGTGCTCCCG
    GGGCGCTGGG ATGACGGGCT CCGCCACCTG GTGACACTCA GCTTCGGGCC TGATCAGCTG
    TGGGGTGTGG GGCAGCAGGT GCACGTGGGC GGCAGGCTCC TCCCCGGTGA CGCCGAGCCC
    TGGGGTGGGC CCTTCCGAGG CTGCATGCAG GACGTGCGAC TCAATGACCT CCACCTCCCC
    TTCTTTCCAC CACTGCTGGG GAACTCCAGC CTGCCCAGCG TGCCCGGCCG CGGGCAGTCC
    TGGAACCTCA CCATGGGCTG CATCTCTGAG GACACATGCA ATCCTGAGCC CTGTCTCAAT
    GGTGGGACTT GCCTCGTCAC CTGGAATGAC TTCCACTGCA CCTGCCCTGC CAACTTCACT
    GGGCCCACGT GTGCCCAGCA GCTGTGGTGT CCTGGCCAGC CCTGTCTCCC GCCTGCCACC
    TGTGAGGAGG TCCCTGATGG CTTTGTCTGT GTGGCGGAGG CCACGTTCCG CGAGGGACCC
    CCGGCGGAAT TCAGCGGGCA CAACGCGTCA TCGGGCAGCG CTCTCAGCGG CCTCTCGCTG
    GTCTTCCGCA CTCGCGACCC CGAGGCAGGG CTGTTGCGCG CTGAGGACGG CCCGGCCGCC
    GTGTGGCTGG CGGTGCGCAA CGGCTCGCTG GCGGCCGGCG TGCGCAGCGG CGCCGGTTTG
    CCCCGCGCGG TGCTGCCAGC GCCCGGGCCG CGTGTGGCCG ACGGCGCCTG GCACCGCGTG
    CGCCTGGCCA TGGAGCAGCC CGCGGCCGTC GCTTCGCGCT GGCTGCTCTG GCTGGACGGC
    GCGGCGACGC CGGTGTCGCT GCGTGGCCTG GCCGGCGACC TGGACTTCCT GCGCGGCCCG
    GGCGCCGCGC GAGTCCTGCT GGCCGAGAAC TTCACGGGCT GCCTGGGCCG CGTGGCGCTC
    GGCGGCCACC CGCTACCCCT GGCGCGCCCG CGGCCCGGCG CGGCCCCCGG CTCCCGCGAG
    CCCTTCTCGG CCCGGCCCGG AGCGCCCGCC CCGCACCTCG GCTGCAGCGG CGCTCCCGTG
    TGTGTCCCCT CGCCCTGCCT GCACGGCGGC GCCTGCCGCG ACCTCTTCGA CGCCTTCGCC
    TGCGCCTGCG GCCCGGGCTG GGAGGGCCCG CGCTGCGAGG ACCGCGCCGA CCCGTGTCGC
    TCGGCGCCCT GCGCCCGCGG CCGCTGCCAC GCACACCCAG ACGGTCACTT CGAGTGCCGC
    TGCCCGCCTG GCTTCGCAGG CCCGCGCTGC AGGTTGCCTG TCCCACCAGA GGGGTGCAGC
    CTGAACATCA CCTGTCTCAA CGGTGGCCAG TGTGAGGAGG GGCCCCAGGG GGCCAACTGC
    AGCTGCCAGG AGAGCTTTGC TGGGCAGAGA TGTCAGATCC CCTGTGAAGC CAACCCCTGC
    TTGAATGGGG GCACCTGCCG GGCAGCTGAT GGGGTGTACG AATGTGTCTG CAATGCCAGG
    TTCTCAGGCC AGTTCTGCGA AGTGGTGAAA GGCCTACCAC TGCCGCTGCC GTTCCCGCTG
    CTGGAGGTAG CAGTGCCCGC GGCCTGCGCC TGCCTCCTCC TCCTCCTCTT GGGCCTCCTC
    TCAGGGATCC TGGCGGCCAG AAAGCGCCGC CAGTCAGAGG GCACCTACAG TCCGAGCCAG
    CAGGAGGTGG CCGGAGCCCG GCTGGAGATG GACAGCGTCC TCAAGGTGCC GCCCGAGGAG
    AGACTCATCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.