DIO1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO1
Entrez Gene ID	782446
Full Name	iodothyronine deiodinase 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the activation, as well as the inactivation of thyroid hormone by outer and inner ring deiodination, respectively. The activation reaction involves the conversion of the prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4), secreted by the thyroid gland, to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by 5'-deiodination. This protein is expressed predominantly in the liver and kidney and provides most of the circulating T3, which is essential for growth, differentiation and basal metabolism in vertebrates. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Apr 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001122593.2	NP_001116065.1	type I iodothyronine deiodinase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DIO1	NP_001116065.1
Rattus norvegicus (Norway rat)	Dio1	NP_067685.5
Gallus gallus (chicken)	DIO1	NP_001091083.1
Danio rerio (zebrafish)	dio1	NP_001007284.2
Xenopus tropicalis (tropical clawed frog)	dio1	NP_001243226.1
Homo sapiens (human)	DIO1	NP_000783.2
Canis lupus familiaris (dog)	DIO1	NP_001007127.1
	DIO1	NP_001116124.1
Mus musculus (house mouse)	Dio1	NP_031886.3
Pan troglodytes (chimpanzee)	DIO1	NP_001116123.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DIO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa40049	NM_001122593.2 Latest version!	Bos taurus iodothyronine deiodinase 1 (DIO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO1 ( NM_001122593.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001116065.1 Bos taurus iodothyronine deiodinase 1 (DIO1), mRNA.

Price & Availability↑

CloneID	OBa40049
Clone ID Related Accession (Same CDS sequence)	NM_001122593.2
Accession Version	NM_001122593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	type I iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DY057291.1, BC103447.1 and EV734437.1. On Apr 29, 2016 this sequence version replaced NM_001122593.1. ##Evidence-Data-START## Transcript exon combination :: BC103447.1, DV812110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGCTGC CCTCGCCAGG GCTATGGCTG AAGAGGCTCT GGGTCCTCTT TCAGGTGGCC 
CTGCATGTGG CCATAGGCAA AGTGCTCCTG ACGCTGTTTC CCAGGAGGGT CAAGCAGAAC 
ATCCTGGCCA TGGGCGAGAA GACTGGCATG ACCAGGAACC CACACTTCTC CCATGAAAAC 
TGGATCCCTA CCTTCTTCAG TACCCAGTAT TTCTGGTTTA TCCTGAAGGT CCGTTGGCAG 
AGACTGGAGG ACATGACTGA ACAAGGGGGT CTGGCCCCAA ACTGTCCTGT AGTCCGCCTG 
TCAGGAGAGA GGTGCAGCAT CTGGGACTTC ATGCAAGGCA ACAGACCCTT GGTGCTGAAT 
TTTGGAAGTT GTACCTGACC TTCATTTATT TTCAAATTTG ACCAATTCAA GAGACTTATT 
GAAGATTTTG GTTCCGTAGC AGATTTTCTC ATCATTTACA TCGAAGAAGC ACATGCATCA 
GATGGCTGGG CTTTTAAGAA CAATGTGGAC ATCAAGAATC ACCGGAATCT CCAGGACCGC 
CTGCGGGCAG CCCACCTGCT GCTGGACAGG AGCCCCCCGT GCCCCGTGGT GGTAGACACA 
ATGACGAACC AGAGCAGCTC GTGCTACGCA GCACTGCCAG AGAGGCTCTA TGTGCTCCAG 
GAGGGCAGGG TCCTGTACAA GGGTAAACCT GGTCCTTGGA ACTACCATCC AGAGGAAGTT 
CGTGCTGTTC TGGAAAAGCT CCACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116065.1
CDS	28..777
Translation	MGLPSPGLWLKRLWVLFQVALHVAIGKVLLTLFPRRVKQNILAMGEKTGMTRNPHFSHENWIPTFFSTQYFWFILKVRWQRLEDMTEQGGLAPNCPVVRLSGERCSIWDFMQGNRPLVLNFGSCT*PSFIFKFDQFKRLIEDFGSVADFLIIYIEEAHASDGWAFKNNVDIKNHRNLQDRLRAAHLLLDRSPPCPVVVDTMTNQSSSCYAALPERLYVLQEGRVLYKGKPGPWNYHPEEVRAVLEKLHS

Target ORF information:

RefSeq Version	NM_001122593.2
Organism	Bos taurus(cattle)
Definition	Bos taurus iodothyronine deiodinase 1 (DIO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122593.2

ORF Insert Sequence:

ATGGGGCTGC CCTCGCCAGG GCTATGGCTG AAGAGGCTCT GGGTCCTCTT TCAGGTGGCC 
CTGCATGTGG CCATAGGCAA AGTGCTCCTG ACGCTGTTTC CCAGGAGGGT CAAGCAGAAC 
ATCCTGGCCA TGGGCGAGAA GACTGGCATG ACCAGGAACC CACACTTCTC CCATGAAAAC 
TGGATCCCTA CCTTCTTCAG TACCCAGTAT TTCTGGTTTA TCCTGAAGGT CCGTTGGCAG 
AGACTGGAGG ACATGACTGA ACAAGGGGGT CTGGCCCCAA ACTGTCCTGT AGTCCGCCTG 
TCAGGAGAGA GGTGCAGCAT CTGGGACTTC ATGCAAGGCA ACAGACCCTT GGTGCTGAAT 
TTTGGAAGTT GTACCTGACC TTCATTTATT TTCAAATTTG ACCAATTCAA GAGACTTATT 
GAAGATTTTG GTTCCGTAGC AGATTTTCTC ATCATTTACA TCGAAGAAGC ACATGCATCA 
GATGGCTGGG CTTTTAAGAA CAATGTGGAC ATCAAGAATC ACCGGAATCT CCAGGACCGC 
CTGCGGGCAG CCCACCTGCT GCTGGACAGG AGCCCCCCGT GCCCCGTGGT GGTAGACACA 
ATGACGAACC AGAGCAGCTC GTGCTACGCA GCACTGCCAG AGAGGCTCTA TGTGCTCCAG 
GAGGGCAGGG TCCTGTACAA GGGTAAACCT GGTCCTTGGA ACTACCATCC AGAGGAAGTT 
CGTGCTGTTC TGGAAAAGCT CCACAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of mammalian selenoproteomes.
Science (New York, N.Y.)300(5624)1439-43(2003 May)
Kryukov GV,Castellano S,Novoselov SV,Lobanov AV,Zehtab O,Guig? R,Gladyshev VN

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DIO1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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