LIG4 cDNA ORF clone, Bos taurus(cattle)

The following LIG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa24943 NM_001191126.1
Latest version!
Bos taurus DNA ligase 4 (LIG4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_024999994.1
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_010810986.3
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_005214000.3
Latest version!
Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_024999995.1
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_010810985.3
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_005213999.3
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_015473933.2
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24944 XM_010810987.2
Latest version!
Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24944 XM_005214003.4
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24944 XM_024999998.1
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa221207 XM_024999997.1
Latest version!
Bos taurus DNA ligase 4 (LIG4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OBa24943 XM_010810985.2 Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_005213999.2 Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_015473933.1 Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24943 XM_010810986.2 Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa24944 XM_005214003.3 Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version NM_001191126.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product DNA ligase 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000012.1. On Jul 17, 2010 this sequence version replaced XM_001249516.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.26476.1, DV836528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145422 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178055.1
    CDS102..2837
    Translation

    Target ORF information:

    RefSeq Version NM_001191126.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191126.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_005214000.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214000.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214057.1
    CDS562..3297
    Translation

    Target ORF information:

    RefSeq Version XM_005214000.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005214000.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_010810986.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810986.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809288.1
    CDS119..2854
    Translation

    Target ORF information:

    RefSeq Version XM_010810986.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810986.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_010810985.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810985.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809287.1
    CDS2624..5359
    Translation

    Target ORF information:

    RefSeq Version XM_010810985.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810985.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_005213999.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214056.1
    CDS317..3052
    Translation

    Target ORF information:

    RefSeq Version XM_005213999.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213999.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_015473933.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329419.1
    CDS328..3063
    Translation

    Target ORF information:

    RefSeq Version XM_015473933.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473933.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_024999995.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855763.1
    CDS49..2784
    Translation

    Target ORF information:

    RefSeq Version XM_024999995.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999995.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_010810985.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809287.1
    CDS2633..5368
    Translation

    Target ORF information:

    RefSeq Version XM_010810985.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810985.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_005213999.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005213999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214056.1
    CDS286..3021
    Translation

    Target ORF information:

    RefSeq Version XM_005213999.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213999.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_015473933.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329419.1
    CDS302..3037
    Translation

    Target ORF information:

    RefSeq Version XM_015473933.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473933.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_010810986.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809288.1
    CDS107..2842
    Translation

    Target ORF information:

    RefSeq Version XM_010810986.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810986.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24943
    Clone ID Related Accession (Same CDS sequence) XM_005214000.3 , XM_010810986.3 , XM_010810985.3 , XM_005213999.3 , XM_015473933.2 , XM_024999995.1 , NM_001191126.1 , XM_010810985.2 , XM_005213999.2 , XM_015473933.1 , XM_010810986.2 , XM_024999994.1
    Accession Version XM_024999994.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855762.1
    CDS221..2956
    Translation

    Target ORF information:

    RefSeq Version XM_024999994.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGGTATT CCTGGGCCAG TGCACCTCTT AGCATGTTTA GACACCTCAC TGTTTATTTG
    GACCTGTACG CTGTCATCAA TGACTTAAGT ACCAGAATCA AGGGAGCTAG GTTAGCCATC
    ACAGCCTTGG AGCTTCGATT TCATGGAGCG CAAGTAGTTT CCTGTTTAGC TGAGGGAGTG
    TCTCACGTGA TTGTTGGGGA GGATCAGAGT CGGGTTGCAG ACTTGAAAGC TATTCGAAGG
    ACTCTTAAGA GGAAATTTAA AATCTTACAA GAACGTTGGG TCACTGTTTC AATAGACAAG
    TGTGAATTAC AGGAGGAAAA TCGGTATTTG GTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24944
    Clone ID Related Accession (Same CDS sequence) XM_010810987.2 , XM_005214003.3 , XM_024999998.1 , XM_005214003.4
    Accession Version XM_010810987.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809289.1
    CDS2754..5288
    Translation

    Target ORF information:

    RefSeq Version XM_010810987.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810987.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24944
    Clone ID Related Accession (Same CDS sequence) XM_010810987.2 , XM_005214003.3 , XM_024999998.1 , XM_005214003.4
    Accession Version XM_005214003.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005214003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214060.1
    CDS220..2754
    Translation

    Target ORF information:

    RefSeq Version XM_005214003.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ligase IV, DNA, ATP-dependent (LIG4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005214003.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24944
    Clone ID Related Accession (Same CDS sequence) XM_010810987.2 , XM_005214003.3 , XM_024999998.1 , XM_005214003.4
    Accession Version XM_024999998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855766.1
    CDS438..2972
    Translation

    Target ORF information:

    RefSeq Version XM_024999998.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24944
    Clone ID Related Accession (Same CDS sequence) XM_010810987.2 , XM_005214003.3 , XM_024999998.1 , XM_005214003.4
    Accession Version XM_005214003.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005214003.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005214060.1
    CDS272..2806
    Translation

    Target ORF information:

    RefSeq Version XM_005214003.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005214003.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTTA TTCTTCCTCA GCTGGAAAGA GAGAGAATGG CCTACGGAAT CAAAGAAACT 
    ATGCTGGCTA AGCTTTATAT TGAATTGCTT AACCTGCCTC GAGATGGAAA AGATGCCCTT
    AAACTTTTGA ACTACAGAAC ACCGACCGGA ACCCGTGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCGTACT TTGTACTGAA ACCCAGGTGT CTACAGAAAG GAAGCCTAAG CATTCAGCAA
    GTAAATGACA TTTTAGACTC CATTGCCAGC AATAATTCTG CCAAAAGGAA AGACCTAATG
    AAGAAGAGTG TTCTTCAGCT TATAACTCAG AGTTCAGCAC TTGAACAAAA GTGGCTGATA
    CGGATGATTG TTAAGGACCT AAAGCTTGGC TTTAGTCAGC AAACTATATT TTCTATTTTT
    CACAGTGATG CTGCCGAGTT GCATAATGTC ACCACAGATC TGGAGAAGGT CTGTAGGCAA
    CTACACGATC CTTCAGTGGG ACTCAGTGAC ATTTCCATCA CCTTATTCTC TGCCTTTAAA
    CCCATGCTGG CCGCTATAGC AGATATCGAG CGAATTGAGA AGGACATGAA ACACCAGAGT
    TTCTACATCG AAACCAAGCT GGATGGCGAG CGGATGCAGA TGCACAAGGA TGGGGATGTG
    TACCGGTACT TCTCTCGCAA TGGGTATAAC TATGTAGACC AGTTTGGTGC TTCCCCACAG
    GAAGGGTCCC TCACACCATT CATTCATAAT GCATTCAAAA CAGAAGTGCT GAACTGTATC
    CTCGATGGTG AGATGATGGC CTACAACCCT AACACACAAA CTTTTATGCA AAAGGGGAAT
    AAGTTTGACA TTAAAAGAAT GGTGGAAGAT TCTGAGCTGC AGACTTGTTA CTGTGTTTTT
    GATGTGTTGA TGGTTAATGA TAAAAAACTA GGACATGAGA CCCTGAGAAA GAGGTATGAA
    ATTCTTAATA GTGTTTTCAC ACCTGTACCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAAAGAAGT CATTGATGCT TTGAATGAAG CGATAGATAA AAGAGAAGAG
    GGGATCATGG TAAAACACCC GCTGTCCATT TACAAGCCGG ATAAAAGAGG TGAAGGATGG
    TTAAAAATTA AACCAGAGTA TGTCAATGGA CTGATGGATG AGCTGGACAT CTTAATCATC
    GGGGGCTACT GGGGGAAGGG TGCCCGGGGC GGGATGATGT CTCATTTTTT GTGCGCTGTG
    TCAGAGAAGC CCCCTTCTGG TGAAAAACCC TCAGTGTTTC ATACTCTGTG TCGCGTGGGC
    TCGGGTTACA CCATGAAAGA ACTGTATGAC CTGGGTTTGA AGTTGGCCAA ACACTGGAAG
    CCTTTTCATA GGAAAGCCCC ACCAAGCAAC ATTCTATGTG GAACAGAGAA GCCAGAGGTC
    TACATCGAGC CTTGCAATTC AGTCATTGTT CAGGTTAAGG CCACAGAGAT CGTCCCCAGT
    GACATGTATA AAACCGGCTG CACGTTGCGT TTTCCACGGA TTGAGAAGAT AAGAGAAGAC
    AAAGAATGGT ACGAATGTAC ATCCCTGGAG GACTTAGAGC AACTTCGAGG GAAAGCCTGT
    GGAAAGCTCG CGTCCAGACA CCTTTACTTG GGTGGTGATG ATGAACCACA AGAGAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTC ATTGGAATTA TTGAGCACCT AAAAGCTCCC
    AACCTTTCTA ACGTAAACAG GGTTTCTACT GTGTTTGAAG ACGTGGAGTT TTGTGTCATG
    AGTGGAACCA GCAGCCATCC CAAGCCTGAC CTGGAGAACA GAATTGCCGA ATTGGGTGGT
    TACATAGTAC AGAACCCAGG CCCAGACACG TACTGTGTGA TCGCAGGGTC TGAGAACATT
    CGAGTGAAGA ATATCATCTC TTCAGATAAA CATGATGTCG TCAAGCCAGA GTGGCTGCTA
    GAGTGTTTTA GGACCAGAAG CTGTGTGCCC TGGCAACCCC GCTTCATGAT CCACATGTGC
    CCGTCGACAA AGCAGCACTT CGCCCAGGAA TACGACCAAT ACGGGGATAG TTACTTTGTC
    GACACAGATT TGCACCAACT GAAGGAAGTG TTTTTGGGAA TTAAAAACGC TGGTGAGCAG
    ACTCCAGGGG AGATGAGTCC TGTGATTGCT GATTTGGAAC ACAGGTATTC CTGGGCCAGT
    GCACCTCTTA GCATGTTTAG ACACCTCACT GTTTATTTGG ACCTGTACGC TGTCATCAAT
    GACTTAAGTA CCAGAATCAA GGGAGCTAGG TTAGCCATCA CAGCCTTGGA GCTTCGATTT
    CATGGAGCGC AAGTAGTTTC CTGTTTAGCT GAGGGAGTGT CTCACGTGAT TGTTGGGGAG
    GATCAGAGTC GGGTTGCAGA CTTGAAAGCT ATTCGAAGGA CTCTTAAGAG GAAATTTAAA
    ATCTTACAAG AACGTTGGGT CACTGTTTCA ATAGACAAGT GTGAATTACA GGAGGAAAAT
    CGGTATTTGG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa221207
    Clone ID Related Accession (Same CDS sequence) XM_024999997.1
    Accession Version XM_024999997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA ligase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGAGCTT TTAATATAAG AAAAAAGATA CTCATGTATA CAAGGAAAAC ACTTAAAAGA
    AAAAAGTGGA TTGTTTATCA AGAAATACAA ATGGTGAAAA GACACCCAGT GGCCCAATGT
    CAAGAAAGAA AAATTTTTAG CAGTATAGTA AGTATTTCAT TATTTTTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855765.1
    CDS317..2887
    Translation

    Target ORF information:

    RefSeq Version XM_024999997.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA ligase 4 (LIG4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGCTGCCT CACAAACCTC ACAAACTGTT GCATCTCATG TTCCTTTTGC AGATTTATGT 
    TCGACTTTAG AACGAATACA GAAAAGTAAA GGGCGTGCAG AAAAAATCAG ACACTTCAAA
    GCATTTTTAG ATTCTTGGAG AAAATTTCAC AATGCCCTTC ATAAGAACCA AAAAGATGTC
    ACAGATTCTT TTTATCCAGC CATGAGACTT ATTCTTCCTC AGCTGGAAAG AGAGAGAATG
    GCCTACGGAA TCAAAGAAAC TATGCTGGCT AAGCTTTATA TTGAATTGCT TAACCTGCCT
    CGAGATGGAA AAGATGCCCT TAAACTTTTG AACTACAGAA CACCGACCGG AACCCGTGGA
    GATGCTGGAG ACTTTGCAAT GATTGCGTAC TTTGTACTGA AACCCAGGTG TCTACAGAAA
    GGAAGCCTAA GCATTCAGCA AGTAAATGAC ATTTTAGACT CCATTGCCAG CAATAATTCT
    GCCAAAAGGA AAGACCTAAT GAAGAAGAGT GTTCTTCAGC TTATAACTCA GAGTTCAGCA
    CTTGAACAAA AGTGGCTGAT ACGGATGATT GTTAAGGACC TAAAGCTTGG CTTTAGTCAG
    CAAACTATAT TTTCTATTTT TCACAGTGAT GCTGCCGAGT TGCATAATGT CACCACAGAT
    CTGGAGAAGG TCTGTAGGCA ACTACACGAT CCTTCAGTGG GACTCAGTGA CATTTCCATC
    ACCTTATTCT CTGCCTTTAA ACCCATGCTG GCCGCTATAG CAGATATCGA GCGAATTGAG
    AAGGACATGA AACACCAGAG TTTCTACATC GAAACCAAGC TGGATGGCGA GCGGATGCAG
    ATGCACAAGG ATGGGGATGT GTACCGGTAC TTCTCTCGCA ATGGGTATAA CTATGTAGAC
    CAGTTTGGTG CTTCCCCACA GGAAGGGTCC CTCACACCAT TCATTCATAA TGCATTCAAA
    ACAGAAGTGC TGAACTGTAT CCTCGATGGT GAGATGATGG CCTACAACCC TAACACACAA
    ACTTTTATGC AAAAGGGGAA TAAGTTTGAC ATTAAAAGAA TGGTGGAAGA TTCTGAGCTG
    CAGACTTGTT ACTGTGTTTT TGATGTGTTG ATGGTTAATG ATAAAAAACT AGGACATGAG
    ACCCTGAGAA AGAGGTATGA AATTCTTAAT AGTGTTTTCA CACCTGTACC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAAAGAAG TCATTGATGC TTTGAATGAA
    GCGATAGATA AAAGAGAAGA GGGGATCATG GTAAAACACC CGCTGTCCAT TTACAAGCCG
    GATAAAAGAG GTGAAGGATG GTTAAAAATT AAACCAGAGT ATGTCAATGG ACTGATGGAT
    GAGCTGGACA TCTTAATCAT CGGGGGCTAC TGGGGGAAGG GTGCCCGGGG CGGGATGATG
    TCTCATTTTT TGTGCGCTGT GTCAGAGAAG CCCCCTTCTG GTGAAAAACC CTCAGTGTTT
    CATACTCTGT GTCGCGTGGG CTCGGGTTAC ACCATGAAAG AACTGTATGA CCTGGGTTTG
    AAGTTGGCCA AACACTGGAA GCCTTTTCAT AGGAAAGCCC CACCAAGCAA CATTCTATGT
    GGAACAGAGA AGCCAGAGGT CTACATCGAG CCTTGCAATT CAGTCATTGT TCAGGTTAAG
    GCCACAGAGA TCGTCCCCAG TGACATGTAT AAAACCGGCT GCACGTTGCG TTTTCCACGG
    ATTGAGAAGA TAAGAGAAGA CAAAGAATGG TACGAATGTA CATCCCTGGA GGACTTAGAG
    CAACTTCGAG GGAAAGCCTG TGGAAAGCTC GCGTCCAGAC ACCTTTACTT GGGTGGTGAT
    GATGAACCAC AAGAGAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT CATTGGAATT
    ATTGAGCACC TAAAAGCTCC CAACCTTTCT AACGTAAACA GGGTTTCTAC TGTGTTTGAA
    GACGTGGAGT TTTGTGTCAT GAGTGGAACC AGCAGCCATC CCAAGCCTGA CCTGGAGAAC
    AGAATTGCCG AATTGGGTGG TTACATAGTA CAGAACCCAG GCCCAGACAC GTACTGTGTG
    ATCGCAGGGT CTGAGAACAT TCGAGTGAAG AATATCATCT CTTCAGATAA ACATGATGTC
    GTCAAGCCAG AGTGGCTGCT AGAGTGTTTT AGGACCAGAA GCTGTGTGCC CTGGCAACCC
    CGCTTCATGA TCCACATGTG CCCGTCGACA AAGCAGCACT TCGCCCAGGA ATACGACCAA
    TACGGGGATA GTTACTTTGT CGACACAGAT TTGCACCAAC TGAAGGAAGT GTTTTTGGGA
    ATTAAAAACG CTGGTGAGCA GACTCCAGGG GAGATGAGTC CTGTGATTGC TGATTTGGAA
    CACAGAGCTT TTAATATAAG AAAAAAGATA CTCATGTATA CAAGGAAAAC ACTTAAAAGA
    AAAAAGTGGA TTGTTTATCA AGAAATACAA ATGGTGAAAA GACACCCAGT GGCCCAATGT
    CAAGAAAGAA AAATTTTTAG CAGTATAGTA AGTATTTCAT TATTTTTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.