ENO4 cDNA ORF clone, Bos taurus(cattle)

The following ENO4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ENO4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39123 NM_001205373.1
Latest version!
Bos taurus enolase 4 (ENO4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa33743 XM_005225754.3
Latest version!
Bos taurus enolase family member 4 (ENO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa56865 XM_010820049.2
Latest version!
Bos taurus enolase family member 4 (ENO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa231003 XM_005225754.4
Latest version!
Bos taurus enolase 4 (ENO4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa231004 XM_010820049.3
Latest version!
Bos taurus enolase 4 (ENO4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa231005 XM_024985828.1
Latest version!
Bos taurus enolase 4 (ENO4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39123
    Clone ID Related Accession (Same CDS sequence) NM_001205373.1
    Accession Version NM_001205373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product enolase 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02059340.1, CB434356.1 and EH206899.1. On or before Apr 20, 2011 this sequence version replaced XR_082726.1, XR_042816.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGGCAAA CTGCTTTTCT
    AAACTTGCAA AACCTCCCAC CATATGCAAA GTTGTGGGGA AAAATGTGCT GGATGGACTT
    GGGCTTCCAA CCCTCCAAGT GGAAATATTC TGCACCATTC AAAATTTTCC CAAGAACATA
    TGTTCTGTGG TGATCGCAAC TCACTTTGAA GCCCACGAGA ACGCTTCACC TGAGCTCGTT
    GAAGCCGAGG AAGTGGAAAG GTTGGATGCT GTCAGCACCG CAGTGCAGTG GGTCAACGAA
    ACTCTCACTG AGGAGCTCCA GGGCCTAGTG CCCTCCAGCC AGGCTGAGGT GGATCAGGTG
    CTCAGGACAT TCTTTGAAAA TAAAGTACAA GAAGATAAGG AGAGAAAAGA ATTGGAAAAG
    AACCTAGAAG ACTCAACAGT GCTTTCACCC CAGCCGCCGC CGCCACCACC TCCTCCTCCA
    CCTCCATCCA AGAAAAAAGG ACAAAAGCAA GGAAGGATGG AGACTTCTGC AGAAAAACCA
    ATCATACCTG CAGAACCCCC TGAGCCTGTC CTCTGTGGCA GCATGGCCAT AGGGGCTGTG
    TCACTGGCTG TTGCCAAAGC CAGTGCTGTA CTGGACAATA ATCCTCTCTA CCTGAACATT
    GCATTACTGA AGCACCACCA GGAACAGCCA GAAAAGCTAA CTATACCTCT TCTGATGGTG
    TCGCTGGTCA GCTGTGGGAA GTCATCACCT GGGAAGCTGA ATTTGATGAA AGAAGTGATC
    TGTATACCCC ATCCTGGATT AACAGCTAAA CAAGGTGTAG AGATGCTTCT GGAAATTCAG
    AAACACATCA ACAAAGCAAT TGAAATGTCC CCTCCTCCAA AGCCAGAGAC AAAAAAAAGT
    CACGATGGAG GCAAAAGAGG CCAACAGCAG ATCACCGGCA AGATGTCCCA TCTTGGCTGT
    TTAACCATTA ATTACGACAC TATAGAGCAG CCACTGCTCC TCATACAAGG AATCTGTGCA
    AACCTGGGGC TAGAATTGGG AACGAACCTG CATCTAGCCA TCAACTGTGC CGCACATGAG
    CTGATGGACT ACAGTAAAGG AAAGTATGAA GTGATGATGG GGACCTACAA AAATGCAGCT
    GAGATGGTTG ACCTGTATGT GGATCTGATC AACAAGTTCC CTTCAATTAT TGCGTTAATT
    GATCCTTTCA GGAAGGAGGA CTCTGAACAG TGGGACAGCA TCTATAATGC TCTTGGTTCC
    AGGTGTTACA TAATTGCAGG CACCGCGTCC AAAAGCATCT CTAAACTGCT AGAGGAGGGC
    AACATCAGCA TCCTCAAATA CAGCGGGCTG ATCATAAAAC ACATAAACCA AACTACGATG
    TCGGACTTGG TGGAAATAAC CAATCTCATT GACGGTAAGA AGCTCATCGC TGTCTTTGGA
    AGCACAGAAG GAGAATCGTC TGATGACAGC CTTGTCGATT TGGCTGTTGG ACTTGGTGTC
    CGGTTTATCA AGTTGGGAGG TCTTTCTCGT GGCGAACGGG TGACCAAGTA CAACCGCCTC
    TTTACTATAG AGGAAGAACT TGGCCAGAAT GGAACACTGG GCTTCAATGA AGAACACACA
    TTTTTCTCCT GGAATGAGGA AGCTGAAGAG GCTGGTGAGC TGCATGAGGT CCCTGAGCCC
    ATCTTCCCCA CAGAAGTTGC GGAGGATCTC GCCAAACTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192302.1
    CDS13..1854
    Translation

    Target ORF information:

    RefSeq Version NM_001205373.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enolase 4 (ENO4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGGCAAA CTGCTTTTCT
    AAACTTGCAA AACCTCCCAC CATATGCAAA GTTGTGGGGA AAAATGTGCT GGATGGACTT
    GGGCTTCCAA CCCTCCAAGT GGAAATATTC TGCACCATTC AAAATTTTCC CAAGAACATA
    TGTTCTGTGG TGATCGCAAC TCACTTTGAA GCCCACGAGA ACGCTTCACC TGAGCTCGTT
    GAAGCCGAGG AAGTGGAAAG GTTGGATGCT GTCAGCACCG CAGTGCAGTG GGTCAACGAA
    ACTCTCACTG AGGAGCTCCA GGGCCTAGTG CCCTCCAGCC AGGCTGAGGT GGATCAGGTG
    CTCAGGACAT TCTTTGAAAA TAAAGTACAA GAAGATAAGG AGAGAAAAGA ATTGGAAAAG
    AACCTAGAAG ACTCAACAGT GCTTTCACCC CAGCCGCCGC CGCCACCACC TCCTCCTCCA
    CCTCCATCCA AGAAAAAAGG ACAAAAGCAA GGAAGGATGG AGACTTCTGC AGAAAAACCA
    ATCATACCTG CAGAACCCCC TGAGCCTGTC CTCTGTGGCA GCATGGCCAT AGGGGCTGTG
    TCACTGGCTG TTGCCAAAGC CAGTGCTGTA CTGGACAATA ATCCTCTCTA CCTGAACATT
    GCATTACTGA AGCACCACCA GGAACAGCCA GAAAAGCTAA CTATACCTCT TCTGATGGTG
    TCGCTGGTCA GCTGTGGGAA GTCATCACCT GGGAAGCTGA ATTTGATGAA AGAAGTGATC
    TGTATACCCC ATCCTGGATT AACAGCTAAA CAAGGTGTAG AGATGCTTCT GGAAATTCAG
    AAACACATCA ACAAAGCAAT TGAAATGTCC CCTCCTCCAA AGCCAGAGAC AAAAAAAAGT
    CACGATGGAG GCAAAAGAGG CCAACAGCAG ATCACCGGCA AGATGTCCCA TCTTGGCTGT
    TTAACCATTA ATTACGACAC TATAGAGCAG CCACTGCTCC TCATACAAGG AATCTGTGCA
    AACCTGGGGC TAGAATTGGG AACGAACCTG CATCTAGCCA TCAACTGTGC CGCACATGAG
    CTGATGGACT ACAGTAAAGG AAAGTATGAA GTGATGATGG GGACCTACAA AAATGCAGCT
    GAGATGGTTG ACCTGTATGT GGATCTGATC AACAAGTTCC CTTCAATTAT TGCGTTAATT
    GATCCTTTCA GGAAGGAGGA CTCTGAACAG TGGGACAGCA TCTATAATGC TCTTGGTTCC
    AGGTGTTACA TAATTGCAGG CACCGCGTCC AAAAGCATCT CTAAACTGCT AGAGGAGGGC
    AACATCAGCA TCCTCAAATA CAGCGGGCTG ATCATAAAAC ACATAAACCA AACTACGATG
    TCGGACTTGG TGGAAATAAC CAATCTCATT GACGGTAAGA AGCTCATCGC TGTCTTTGGA
    AGCACAGAAG GAGAATCGTC TGATGACAGC CTTGTCGATT TGGCTGTTGG ACTTGGTGTC
    CGGTTTATCA AGTTGGGAGG TCTTTCTCGT GGCGAACGGG TGACCAAGTA CAACCGCCTC
    TTTACTATAG AGGAAGAACT TGGCCAGAAT GGAACACTGG GCTTCAATGA AGAACACACA
    TTTTTCTCCT GGAATGAGGA AGCTGAAGAG GCTGGTGAGC TGCATGAGGT CCCTGAGCCC
    ATCTTCCCCA CAGAAGTTGC GGAGGATCTC GCCAAACTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33743
    Clone ID Related Accession (Same CDS sequence) XM_005225754.3
    Accession Version XM_005225754.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product enolase-like protein ENO4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225754.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGGCAAA CTGCTTTTCT
    AAACTTGCAA AACCTCCCAC CATATGCAAA GTTGTGGGGA AAAATGTGCT GGATGGACTT
    GGGCTTCCAA CCCTCCAAGT GGAAATATTC TGCACCATTC AAAATTTTCC CAAGAACATA
    TGTTCTGTGG TGATCGCAAC TCACTTTGAA GCCCACGAGA ACGCTTCACC TGAGCTCGTT
    GAAGCCGAGG AAGTGGAAAG GTTGGATGCT GTCAGCACCG CAGTGCAGTG GGTCAACGAA
    ACTCTCACTG AGGAGCTCCA GGGCCTAGTG CCCTCCAGCC AGGCTGAGGT GGATCAGGTG
    CTCAGGACAT TCTTTGAAAA TAAAGTACAA GAAGATAAGG AGAGAAAAGA ATTGGAAAAG
    AACCTAGAAG ACTCAACAGT GCTTTCACCC CAGCCGCCGC CGCCACCACC TCCTCCTCCA
    CCTCCATCCA AGAAAAAAGG ACAAAAGCAA GGAAGGATGG AGACTTCTGC AGAAAAACCA
    ATCATACCTG CAGAACCCCC TGAGCCTGTC CTCTGTGGCA GCATGGCCAT AGGGGCTGTG
    TCACTGGCTG TTGCCAAAGC CAGTGCTGTA CTGGACAATA ATCCTCTCTA CCTGAACATT
    GCATTACTGA AGCACCACCA GGAACAGCCA GAAAAGCTAA CTATACCTCT TCTGATGGTG
    TCGCTGGTCA GCTGTGGGAA GTCATCACCT GGGAAGCTGA ATTTGATGAA AGAAGTGATC
    TGTATACCCC ATCCTGGATT AACAGCTAAA CAATCCCCTC CTCCAAAGCC AGAGACAAAA
    AAAAGTCACG ATGGAGGCAA AAGAGGCCAA CAGCAGATCA CCGGCAAGAT GTCCCATCTT
    GGCTGTTTAA CCATTAATTA CGACACTATA GAGCAGCCAC TGCTCCTCAT ACAAGGAATC
    TGTGCAAACC TGGGGCTAGA ATTGGGAACG AACCTGCATC TAGCCATCAA CTGTGCCGCA
    CATGAGCTGA TGGACTACAG TAAAGGAAAG TATGAAGTGA TGATGGGGAC CTACAAAAAT
    GCAGCTGAGA TGGTTGACCT GTATGTGGAT CTGATCAACA AGTTCCCTTC AATTATTGCG
    TTAATTGATC CTTTCAGGAA GGAGGACTCT GAACAGTGGG ACAGCATCTA TAATGCTCTT
    GGTTCCAGGT GTTACATAAT TGCAGGCACC GCGTCCAAAA GCATCTCTAA ACTGCTAGAG
    GAGGGCAACA TCAGCATCCT CAAATACAGC GGGCTGATCA TAAAACACAT AAACCAAACT
    ACGATGTCGG ACTTGGTGGA AATAACCAAT CTCATTGACG GTAAGAAGCT CATCGCTGTC
    TTTGGAAGCA CAGAAGGAGA ATCGTCTGAT GACAGCCTTG TCGATTTGGC TGTTGGACTT
    GGTGTCCGGT TTATCAAGTT GGGAGGTCTT TCTCGTGGCG AACGGGTGAC CAAGTACAAC
    CGCCTCTTTA CTATAGAGGA AGAACTTGGC CAGAATGGAA CACTGGGCTT CAATGAAGAA
    CACACATTTT TCTCCTGGAA TGAGGAAGCT GAAGAGGCTG GTGAGCTGCA TGAGGTCCCT
    GAGCCCATCT TCCCCACAGA AGTTGCGGAG GATCTCGCCA AACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225811.1
    CDS194..1981
    Translation

    Target ORF information:

    RefSeq Version XM_005225754.3
    Organism Bos taurus(cattle)
    Definition Bos taurus enolase family member 4 (ENO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225754.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGGCAAA CTGCTTTTCT
    AAACTTGCAA AACCTCCCAC CATATGCAAA GTTGTGGGGA AAAATGTGCT GGATGGACTT
    GGGCTTCCAA CCCTCCAAGT GGAAATATTC TGCACCATTC AAAATTTTCC CAAGAACATA
    TGTTCTGTGG TGATCGCAAC TCACTTTGAA GCCCACGAGA ACGCTTCACC TGAGCTCGTT
    GAAGCCGAGG AAGTGGAAAG GTTGGATGCT GTCAGCACCG CAGTGCAGTG GGTCAACGAA
    ACTCTCACTG AGGAGCTCCA GGGCCTAGTG CCCTCCAGCC AGGCTGAGGT GGATCAGGTG
    CTCAGGACAT TCTTTGAAAA TAAAGTACAA GAAGATAAGG AGAGAAAAGA ATTGGAAAAG
    AACCTAGAAG ACTCAACAGT GCTTTCACCC CAGCCGCCGC CGCCACCACC TCCTCCTCCA
    CCTCCATCCA AGAAAAAAGG ACAAAAGCAA GGAAGGATGG AGACTTCTGC AGAAAAACCA
    ATCATACCTG CAGAACCCCC TGAGCCTGTC CTCTGTGGCA GCATGGCCAT AGGGGCTGTG
    TCACTGGCTG TTGCCAAAGC CAGTGCTGTA CTGGACAATA ATCCTCTCTA CCTGAACATT
    GCATTACTGA AGCACCACCA GGAACAGCCA GAAAAGCTAA CTATACCTCT TCTGATGGTG
    TCGCTGGTCA GCTGTGGGAA GTCATCACCT GGGAAGCTGA ATTTGATGAA AGAAGTGATC
    TGTATACCCC ATCCTGGATT AACAGCTAAA CAATCCCCTC CTCCAAAGCC AGAGACAAAA
    AAAAGTCACG ATGGAGGCAA AAGAGGCCAA CAGCAGATCA CCGGCAAGAT GTCCCATCTT
    GGCTGTTTAA CCATTAATTA CGACACTATA GAGCAGCCAC TGCTCCTCAT ACAAGGAATC
    TGTGCAAACC TGGGGCTAGA ATTGGGAACG AACCTGCATC TAGCCATCAA CTGTGCCGCA
    CATGAGCTGA TGGACTACAG TAAAGGAAAG TATGAAGTGA TGATGGGGAC CTACAAAAAT
    GCAGCTGAGA TGGTTGACCT GTATGTGGAT CTGATCAACA AGTTCCCTTC AATTATTGCG
    TTAATTGATC CTTTCAGGAA GGAGGACTCT GAACAGTGGG ACAGCATCTA TAATGCTCTT
    GGTTCCAGGT GTTACATAAT TGCAGGCACC GCGTCCAAAA GCATCTCTAA ACTGCTAGAG
    GAGGGCAACA TCAGCATCCT CAAATACAGC GGGCTGATCA TAAAACACAT AAACCAAACT
    ACGATGTCGG ACTTGGTGGA AATAACCAAT CTCATTGACG GTAAGAAGCT CATCGCTGTC
    TTTGGAAGCA CAGAAGGAGA ATCGTCTGAT GACAGCCTTG TCGATTTGGC TGTTGGACTT
    GGTGTCCGGT TTATCAAGTT GGGAGGTCTT TCTCGTGGCG AACGGGTGAC CAAGTACAAC
    CGCCTCTTTA CTATAGAGGA AGAACTTGGC CAGAATGGAA CACTGGGCTT CAATGAAGAA
    CACACATTTT TCTCCTGGAA TGAGGAAGCT GAAGAGGCTG GTGAGCTGCA TGAGGTCCCT
    GAGCCCATCT TCCCCACAGA AGTTGCGGAG GATCTCGCCA AACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa56865
    Clone ID Related Accession (Same CDS sequence) XM_010820049.2
    Accession Version XM_010820049.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product enolase-like protein ENO4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104583.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820049.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACAGTTC TTCATTCCTG CTTCTCTTCC TCAAGTCTTG CCGGTGACAC CTCCACCCTA 
    GGCCCGGTCC TTAAGGCAAA CTGCTTTTCT AAACTTGCAA AACCTCCCAC CATATGCAAA
    GTTGTGGGGA AAAATGTGCT GGATGGACTT GGGCTTCCAA CCCTCCAAGT GGAAATATTC
    TGCACCATTC AAAATTTTCC CAAGAACATA TGTTCTGTGG TGATCGCAAC TCACTTTGAA
    GCCCACGAGA ACGCTTCACC TGAGCTCGTT GAAGCCGAGG AAGTGGAAAG GTTGGATGCT
    GTCAGCACCG CAGTGCAGTG GGTCAACGAA ACTCTCACTG AGGAGCTCCA GGGCCTAGTG
    CCCTCCAGCC AGGCTGAGGT GGATCAGGTG CTCAGGACAT TCTTTGAAAA TAAAGTACAA
    GAAGATAAGG AGAGAAAAGA ATTGGAAAAG AACCTAGAAG ACTCAACAGT GCTTTCACCC
    CAGCCGCCGC CGCCACCACC TCCTCCTCCA CCTCCATCCA AGAAAAAAGG ACAAAAGCAA
    GGAAGGATGG AGACTTCTGC AGAAAAACCA ATCATACCTG CAGAACCCCC TGAGCCTGTC
    CTCTGTGGCA GCATGGCCAT AGGGGCTGTG TCACTGGCTG TTGCCAAAGC CAGTGCTGTA
    CTGGACAATA ATCCTCTCTA CCTGAACATT GCATTACTGA AGCACCACCA GGAACAGCCA
    GAAAAGCTAA CTATACCTCT TCTGATGGTG TCGCTGGTCA GCTGTGGGAA GTCATCACCT
    GGGAAGCTGA ATTTGATGAA AGAAGTGATC TGTATACCCC ATCCTGGATT AACAGCTAAA
    CAAGGTGTAG AGATGCTTCT GGAAATTCAG AAACACATCA ACAAAGCAAT TGAAATGTCC
    CCTCCTCCAA AGCCAGAGAC AAAAAAAAGT CACGATGGAG GCAAAAGAGG CCAACAGCAG
    ATCACCGGCA AGATGTCCCA TCTTGGCTGT TTAACCATTA ATTACGACAC TATAGAGCAG
    CCACTGCTCC TCATACAAGG AATCTGTGCA AACCTGGGGC TAGAATTGGG AACGAACCTG
    CATCTAGCCA TCAACTGTGC CGCACATGAG CTGATGGACT ACAGTAAAGG AAAGTATGAA
    GTGATGATGG GGACCTACAA AAATGCAGCT GAGATGGTTG ACCTGTATGT GGATCTGATC
    AACAAGTTCC CTTCAATTAT TGCGTTAATT GATCCTTTCA GGAAGGAGGA CTCTGAACAG
    TGGGACAGCA TCTATAATGC TCTTGGTTCC AGGTGTTACA TAATTGCAGG CACCGCGTCC
    AAAAGCATCT CTAAACTGCT AGAGGAGGGC AACATCAGCA TCCTCAAATA CAGCGGGCTG
    ATCATAAAAC ACATAAACCA AACTACGATG TCGGACTTGG TGGAAATAAC CAATCTCATT
    GACGGTAAGA AGCTCATCGC TGTCTTTGGA AGCACAGAAG GAGAATCGTC TGATGACAGC
    CTTGTCGATT TGGCTGTTGG ACTTGGTGTC CGGTTTATCA AGTTGGGAGG TCTTTCTCGT
    GGCGAACGGG TGACCAAGTA CAACCGCCTC TTTACTATAG AGGAAGAACT TGGCCAGAAT
    GGAACACTGG GCTTCAATGA AGAACACACA TTTTTCTCCT GGAATGAGGA AGCTGAAGAG
    GCTGGTGAGC TGCATGAGGT CCCTGAGCCC ATCTTCCCCA CAGAAGTTGC GGAGGATCTC
    GCCAAACTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818351.1
    CDS1607..3358
    Translation

    Target ORF information:

    RefSeq Version XM_010820049.2
    Organism Bos taurus(cattle)
    Definition Bos taurus enolase family member 4 (ENO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820049.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACAGTTC TTCATTCCTG CTTCTCTTCC TCAAGTCTTG CCGGTGACAC CTCCACCCTA 
    GGCCCGGTCC TTAAGGCAAA CTGCTTTTCT AAACTTGCAA AACCTCCCAC CATATGCAAA
    GTTGTGGGGA AAAATGTGCT GGATGGACTT GGGCTTCCAA CCCTCCAAGT GGAAATATTC
    TGCACCATTC AAAATTTTCC CAAGAACATA TGTTCTGTGG TGATCGCAAC TCACTTTGAA
    GCCCACGAGA ACGCTTCACC TGAGCTCGTT GAAGCCGAGG AAGTGGAAAG GTTGGATGCT
    GTCAGCACCG CAGTGCAGTG GGTCAACGAA ACTCTCACTG AGGAGCTCCA GGGCCTAGTG
    CCCTCCAGCC AGGCTGAGGT GGATCAGGTG CTCAGGACAT TCTTTGAAAA TAAAGTACAA
    GAAGATAAGG AGAGAAAAGA ATTGGAAAAG AACCTAGAAG ACTCAACAGT GCTTTCACCC
    CAGCCGCCGC CGCCACCACC TCCTCCTCCA CCTCCATCCA AGAAAAAAGG ACAAAAGCAA
    GGAAGGATGG AGACTTCTGC AGAAAAACCA ATCATACCTG CAGAACCCCC TGAGCCTGTC
    CTCTGTGGCA GCATGGCCAT AGGGGCTGTG TCACTGGCTG TTGCCAAAGC CAGTGCTGTA
    CTGGACAATA ATCCTCTCTA CCTGAACATT GCATTACTGA AGCACCACCA GGAACAGCCA
    GAAAAGCTAA CTATACCTCT TCTGATGGTG TCGCTGGTCA GCTGTGGGAA GTCATCACCT
    GGGAAGCTGA ATTTGATGAA AGAAGTGATC TGTATACCCC ATCCTGGATT AACAGCTAAA
    CAAGGTGTAG AGATGCTTCT GGAAATTCAG AAACACATCA ACAAAGCAAT TGAAATGTCC
    CCTCCTCCAA AGCCAGAGAC AAAAAAAAGT CACGATGGAG GCAAAAGAGG CCAACAGCAG
    ATCACCGGCA AGATGTCCCA TCTTGGCTGT TTAACCATTA ATTACGACAC TATAGAGCAG
    CCACTGCTCC TCATACAAGG AATCTGTGCA AACCTGGGGC TAGAATTGGG AACGAACCTG
    CATCTAGCCA TCAACTGTGC CGCACATGAG CTGATGGACT ACAGTAAAGG AAAGTATGAA
    GTGATGATGG GGACCTACAA AAATGCAGCT GAGATGGTTG ACCTGTATGT GGATCTGATC
    AACAAGTTCC CTTCAATTAT TGCGTTAATT GATCCTTTCA GGAAGGAGGA CTCTGAACAG
    TGGGACAGCA TCTATAATGC TCTTGGTTCC AGGTGTTACA TAATTGCAGG CACCGCGTCC
    AAAAGCATCT CTAAACTGCT AGAGGAGGGC AACATCAGCA TCCTCAAATA CAGCGGGCTG
    ATCATAAAAC ACATAAACCA AACTACGATG TCGGACTTGG TGGAAATAAC CAATCTCATT
    GACGGTAAGA AGCTCATCGC TGTCTTTGGA AGCACAGAAG GAGAATCGTC TGATGACAGC
    CTTGTCGATT TGGCTGTTGG ACTTGGTGTC CGGTTTATCA AGTTGGGAGG TCTTTCTCGT
    GGCGAACGGG TGACCAAGTA CAACCGCCTC TTTACTATAG AGGAAGAACT TGGCCAGAAT
    GGAACACTGG GCTTCAATGA AGAACACACA TTTTTCTCCT GGAATGAGGA AGCTGAAGAG
    GCTGGTGAGC TGCATGAGGT CCCTGAGCCC ATCTTCCCCA CAGAAGTTGC GGAGGATCTC
    GCCAAACTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231003
    Clone ID Related Accession (Same CDS sequence) XM_005225754.4
    Accession Version XM_005225754.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product enolase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225754.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGGCAAA CTGCTTTTCT
    AAACTTGCAA AACCTCCCAC CATATGCAAA GTTGTGGGGA AAAATGTGCT GGATGGACTT
    GGGCTTCCAA CCCTCCAAGT GGAAATATTC TGCACCATTC AAAATTTTCC CAAGAACATA
    TGTTCTGTGG TGATCGCAAC TCACTTTGAA GCCCACGAGA ACGCTTCACC TGAGCTCGTT
    GAAGCCGAGG AAGTGGAAAG GTTGGATGCT GTCAGCACCG CAGTGCAGTG GGTCAACGAA
    ACTCTCACTG AGGAGCTCCA GGGCCTAGTG CCCTCCAGCC AGGCTGAGGT GGATCAGGTG
    CTCAGGACAT TCTTTGAAAA TAAAGTACAA GAAGATAAGG AGAGAAAAGA ATTGGAAAAG
    AACCTAGAAG ACTCAACAGT GCTTTCACCC CAGCCGCCGC CGCCACCACC TCCTCCTCCA
    CCTCCATCCA AGAAAAAAGG ACAAAAGCAA GGAAGGATGG AGACTTCTGC AGAAAAACCA
    ATCATACCTG CAGAACCCCC TGAGCCTGTC CTCTGTGGCA GCATGGCCAT AGGGGCTGTG
    TCACTGGCTG TTGCCAAAGC CAGTGCTGTA CTGGACAATA ATCCTCTCTA CCTGAACATT
    GCATTACTGA AGCACCACCA GGAACAGCCA AAAAAGCTAA CTATACCTCT TCTGATGGTG
    TCGCTGGTCA GCTGTGGGAA GTCATCACCT GGGAAGCTGA ATTTGATGAA AGAAGTGATC
    TGTATACCCC ATCCTGGATT AACAGCTAAA CAATCCCCTC CTCCAAAGCC AGAGACAAAA
    AAAAGTCACG ATGGAGGCAA AAGAGGCCAA CAGCAGATCA CCGGCAAGAT GTCCCATCTT
    GGCTGTTTAA CCATTAATTA CGACACTATA GAGCAGCCAC TGCTCCTCAT ACAAGGAATC
    TGTGCAAACC TGGGGCTAGA ATTGGGAACG AACCTGCATC TAGCCATCAA CTGTGCCGCA
    CATGAGCTGA TGGACTACAG TAAAGGAAAG TATGAAGTGA TGATGGGGAC CTACAAAAAT
    GCAGCTGAGA TGGTTGACCT GTATGTGGAT CTGATCAACA AGTTCCCTTC AATTATTGCG
    TTAATTGATC CTTTCAGGAA GGAGGACTCT GAACAGTGGG ACAGCATCTA TAATGCTCTT
    GGTTCCAGGT GTTACATAAT TGCAGGCACC GCGTCCAAAA GCATCTCTAA ACTGCTAGAG
    GAGGGCAACA TCAGCATCCT CAAATACAGC GGGCTGATCA TAAAACACAT AAACCAAACT
    ACGATGTCGG ACTTGGTGGA AATAACCAAT CTCATTGACG GTAAGAAGCT CATCGCTGTC
    TTTGGAAGCA CAGAAGGAGA ATCGTCTGAT GACAGCCTTG TCGATTTGGC TGTTGGACTT
    GGTGTCCGGT TTATCAAGTT GGGAGGTCTT TCTCGTGGCG AACGGGTGAC CAAGTACAAC
    CGCCTCTTTA CTATAGAGGA AGAACTTGGC CAGAATGGAA CACTGGGCTT CAATGAAGAA
    CACACATTTT TCTCCTGGAA TGAGGAAGCT GAAGAGGCTG GTGAGCTGCA TGAGGTCCCT
    GAGCCCATCT TCCCCACAGA AGTTGCGGAG GATCTCGCCA AACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225811.2
    CDS583..2370
    Translation

    Target ORF information:

    RefSeq Version XM_005225754.4
    Organism Bos taurus(cattle)
    Definition Bos taurus enolase 4 (ENO4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225754.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGGCAAA CTGCTTTTCT
    AAACTTGCAA AACCTCCCAC CATATGCAAA GTTGTGGGGA AAAATGTGCT GGATGGACTT
    GGGCTTCCAA CCCTCCAAGT GGAAATATTC TGCACCATTC AAAATTTTCC CAAGAACATA
    TGTTCTGTGG TGATCGCAAC TCACTTTGAA GCCCACGAGA ACGCTTCACC TGAGCTCGTT
    GAAGCCGAGG AAGTGGAAAG GTTGGATGCT GTCAGCACCG CAGTGCAGTG GGTCAACGAA
    ACTCTCACTG AGGAGCTCCA GGGCCTAGTG CCCTCCAGCC AGGCTGAGGT GGATCAGGTG
    CTCAGGACAT TCTTTGAAAA TAAAGTACAA GAAGATAAGG AGAGAAAAGA ATTGGAAAAG
    AACCTAGAAG ACTCAACAGT GCTTTCACCC CAGCCGCCGC CGCCACCACC TCCTCCTCCA
    CCTCCATCCA AGAAAAAAGG ACAAAAGCAA GGAAGGATGG AGACTTCTGC AGAAAAACCA
    ATCATACCTG CAGAACCCCC TGAGCCTGTC CTCTGTGGCA GCATGGCCAT AGGGGCTGTG
    TCACTGGCTG TTGCCAAAGC CAGTGCTGTA CTGGACAATA ATCCTCTCTA CCTGAACATT
    GCATTACTGA AGCACCACCA GGAACAGCCA AAAAAGCTAA CTATACCTCT TCTGATGGTG
    TCGCTGGTCA GCTGTGGGAA GTCATCACCT GGGAAGCTGA ATTTGATGAA AGAAGTGATC
    TGTATACCCC ATCCTGGATT AACAGCTAAA CAATCCCCTC CTCCAAAGCC AGAGACAAAA
    AAAAGTCACG ATGGAGGCAA AAGAGGCCAA CAGCAGATCA CCGGCAAGAT GTCCCATCTT
    GGCTGTTTAA CCATTAATTA CGACACTATA GAGCAGCCAC TGCTCCTCAT ACAAGGAATC
    TGTGCAAACC TGGGGCTAGA ATTGGGAACG AACCTGCATC TAGCCATCAA CTGTGCCGCA
    CATGAGCTGA TGGACTACAG TAAAGGAAAG TATGAAGTGA TGATGGGGAC CTACAAAAAT
    GCAGCTGAGA TGGTTGACCT GTATGTGGAT CTGATCAACA AGTTCCCTTC AATTATTGCG
    TTAATTGATC CTTTCAGGAA GGAGGACTCT GAACAGTGGG ACAGCATCTA TAATGCTCTT
    GGTTCCAGGT GTTACATAAT TGCAGGCACC GCGTCCAAAA GCATCTCTAA ACTGCTAGAG
    GAGGGCAACA TCAGCATCCT CAAATACAGC GGGCTGATCA TAAAACACAT AAACCAAACT
    ACGATGTCGG ACTTGGTGGA AATAACCAAT CTCATTGACG GTAAGAAGCT CATCGCTGTC
    TTTGGAAGCA CAGAAGGAGA ATCGTCTGAT GACAGCCTTG TCGATTTGGC TGTTGGACTT
    GGTGTCCGGT TTATCAAGTT GGGAGGTCTT TCTCGTGGCG AACGGGTGAC CAAGTACAAC
    CGCCTCTTTA CTATAGAGGA AGAACTTGGC CAGAATGGAA CACTGGGCTT CAATGAAGAA
    CACACATTTT TCTCCTGGAA TGAGGAAGCT GAAGAGGCTG GTGAGCTGCA TGAGGTCCCT
    GAGCCCATCT TCCCCACAGA AGTTGCGGAG GATCTCGCCA AACTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231004
    Clone ID Related Accession (Same CDS sequence) XM_010820049.3
    Accession Version XM_010820049.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product enolase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACAGTTC TTCATTCCTG CTTCTCTTCC TCAAGTCTTG CCGGTGACAC CTCCACCCTA 
    GGCCCGGTCC TTAAGGCAAA CTGCTTTTCT AAACTTGCAA AACCTCCCAC CATATGCAAA
    GTTGTGGGGA AAAATGTGCT GGATGGACTT GGGCTTCCAA CCCTCCAAGT GGAAATATTC
    TGCACCATTC AAAATTTTCC CAAGAACATA TGTTCTGTGG TGATCGCAAC TCACTTTGAA
    GCCCACGAGA ACGCTTCACC TGAGCTCGTT GAAGCCGAGG AAGTGGAAAG GTTGGATGCT
    GTCAGCACCG CAGTGCAGTG GGTCAACGAA ACTCTCACTG AGGAGCTCCA GGGCCTAGTG
    CCCTCCAGCC AGGCTGAGGT GGATCAGGTG CTCAGGACAT TCTTTGAAAA TAAAGTACAA
    GAAGATAAGG AGAGAAAAGA ATTGGAAAAG AACCTAGAAG ACTCAACAGT GCTTTCACCC
    CAGCCGCCGC CGCCACCACC TCCTCCTCCA CCTCCATCCA AGAAAAAAGG ACAAAAGCAA
    GGAAGGATGG AGACTTCTGC AGAAAAACCA ATCATACCTG CAGAACCCCC TGAGCCTGTC
    CTCTGTGGCA GCATGGCCAT AGGGGCTGTG TCACTGGCTG TTGCCAAAGC CAGTGCTGTA
    CTGGACAATA ATCCTCTCTA CCTGAACATT GCATTACTGA AGCACCACCA GGAACAGCCA
    AAAAAGCTAA CTATACCTCT TCTGATGGTG TCGCTGGTCA GCTGTGGGAA GTCATCACCT
    GGGAAGCTGA ATTTGATGAA AGAAGTGATC TGTATACCCC ATCCTGGATT AACAGCTAAA
    CAAGGTGTAG AGATGCTTCT GGAAATTCAG AAACACATCA ACAAAGCAAT TGAAATGTCC
    CCTCCTCCAA AGCCAGAGAC AAAAAAAAGT CACGATGGAG GCAAAAGAGG CCAACAGCAG
    ATCACCGGCA AGATGTCCCA TCTTGGCTGT TTAACCATTA ATTACGACAC TATAGAGCAG
    CCACTGCTCC TCATACAAGG AATCTGTGCA AACCTGGGGC TAGAATTGGG AACGAACCTG
    CATCTAGCCA TCAACTGTGC CGCACATGAG CTGATGGACT ACAGTAAAGG AAAGTATGAA
    GTGATGATGG GGACCTACAA AAATGCAGCT GAGATGGTTG ACCTGTATGT GGATCTGATC
    AACAAGTTCC CTTCAATTAT TGCGTTAATT GATCCTTTCA GGAAGGAGGA CTCTGAACAG
    TGGGACAGCA TCTATAATGC TCTTGGTTCC AGGTGTTACA TAATTGCAGG CACCGCGTCC
    AAAAGCATCT CTAAACTGCT AGAGGAGGGC AACATCAGCA TCCTCAAATA CAGCGGGCTG
    ATCATAAAAC ACATAAACCA AACTACGATG TCGGACTTGG TGGAAATAAC CAATCTCATT
    GACGGTAAGA AGCTCATCGC TGTCTTTGGA AGCACAGAAG GAGAATCGTC TGATGACAGC
    CTTGTCGATT TGGCTGTTGG ACTTGGTGTC CGGTTTATCA AGTTGGGAGG TCTTTCTCGT
    GGCGAACGGG TGACCAAGTA CAACCGCCTC TTTACTATAG AGGAAGAACT TGGCCAGAAT
    GGAACACTGG GCTTCAATGA AGAACACACA TTTTTCTCCT GGAATGAGGA AGCTGAAGAG
    GCTGGTGAGC TGCATGAGGT CCCTGAGCCC ATCTTCCCCA CAGAAGTTGC GGAGGATCTC
    GCCAAACTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818351.2
    CDS2590..4341
    Translation

    Target ORF information:

    RefSeq Version XM_010820049.3
    Organism Bos taurus(cattle)
    Definition Bos taurus enolase 4 (ENO4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820049.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGACAGTTC TTCATTCCTG CTTCTCTTCC TCAAGTCTTG CCGGTGACAC CTCCACCCTA 
    GGCCCGGTCC TTAAGGCAAA CTGCTTTTCT AAACTTGCAA AACCTCCCAC CATATGCAAA
    GTTGTGGGGA AAAATGTGCT GGATGGACTT GGGCTTCCAA CCCTCCAAGT GGAAATATTC
    TGCACCATTC AAAATTTTCC CAAGAACATA TGTTCTGTGG TGATCGCAAC TCACTTTGAA
    GCCCACGAGA ACGCTTCACC TGAGCTCGTT GAAGCCGAGG AAGTGGAAAG GTTGGATGCT
    GTCAGCACCG CAGTGCAGTG GGTCAACGAA ACTCTCACTG AGGAGCTCCA GGGCCTAGTG
    CCCTCCAGCC AGGCTGAGGT GGATCAGGTG CTCAGGACAT TCTTTGAAAA TAAAGTACAA
    GAAGATAAGG AGAGAAAAGA ATTGGAAAAG AACCTAGAAG ACTCAACAGT GCTTTCACCC
    CAGCCGCCGC CGCCACCACC TCCTCCTCCA CCTCCATCCA AGAAAAAAGG ACAAAAGCAA
    GGAAGGATGG AGACTTCTGC AGAAAAACCA ATCATACCTG CAGAACCCCC TGAGCCTGTC
    CTCTGTGGCA GCATGGCCAT AGGGGCTGTG TCACTGGCTG TTGCCAAAGC CAGTGCTGTA
    CTGGACAATA ATCCTCTCTA CCTGAACATT GCATTACTGA AGCACCACCA GGAACAGCCA
    AAAAAGCTAA CTATACCTCT TCTGATGGTG TCGCTGGTCA GCTGTGGGAA GTCATCACCT
    GGGAAGCTGA ATTTGATGAA AGAAGTGATC TGTATACCCC ATCCTGGATT AACAGCTAAA
    CAAGGTGTAG AGATGCTTCT GGAAATTCAG AAACACATCA ACAAAGCAAT TGAAATGTCC
    CCTCCTCCAA AGCCAGAGAC AAAAAAAAGT CACGATGGAG GCAAAAGAGG CCAACAGCAG
    ATCACCGGCA AGATGTCCCA TCTTGGCTGT TTAACCATTA ATTACGACAC TATAGAGCAG
    CCACTGCTCC TCATACAAGG AATCTGTGCA AACCTGGGGC TAGAATTGGG AACGAACCTG
    CATCTAGCCA TCAACTGTGC CGCACATGAG CTGATGGACT ACAGTAAAGG AAAGTATGAA
    GTGATGATGG GGACCTACAA AAATGCAGCT GAGATGGTTG ACCTGTATGT GGATCTGATC
    AACAAGTTCC CTTCAATTAT TGCGTTAATT GATCCTTTCA GGAAGGAGGA CTCTGAACAG
    TGGGACAGCA TCTATAATGC TCTTGGTTCC AGGTGTTACA TAATTGCAGG CACCGCGTCC
    AAAAGCATCT CTAAACTGCT AGAGGAGGGC AACATCAGCA TCCTCAAATA CAGCGGGCTG
    ATCATAAAAC ACATAAACCA AACTACGATG TCGGACTTGG TGGAAATAAC CAATCTCATT
    GACGGTAAGA AGCTCATCGC TGTCTTTGGA AGCACAGAAG GAGAATCGTC TGATGACAGC
    CTTGTCGATT TGGCTGTTGG ACTTGGTGTC CGGTTTATCA AGTTGGGAGG TCTTTCTCGT
    GGCGAACGGG TGACCAAGTA CAACCGCCTC TTTACTATAG AGGAAGAACT TGGCCAGAAT
    GGAACACTGG GCTTCAATGA AGAACACACA TTTTTCTCCT GGAATGAGGA AGCTGAAGAG
    GCTGGTGAGC TGCATGAGGT CCCTGAGCCC ATCTTCCCCA CAGAAGTTGC GGAGGATCTC
    GCCAAACTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231005
    Clone ID Related Accession (Same CDS sequence) XM_024985828.1
    Accession Version XM_024985828.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1713bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product enolase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGAACAT ATGTTCTGTG
    GTGATCGCAA CTCACTTTGA AGCCCACGAG AACGCTTCAC CTGAGCTCGT TGAAGCCGAG
    GAAGTGGAAA GGTTGGATGC TGTCAGCACC GCAGTGCAGT GGGTCAACGA AACTCTCACT
    GAGGAGCTCC AGGGCCTAGT GCCCTCCAGC CAGGCTGAGG TGGATCAGGT GCTCAGGACA
    TTCTTTGAAA ATAAAGTACA AGAAGATAAG GAGAGAAAAG AATTGGAAAA GAACCTAGAA
    GACTCAACAG TGCTTTCACC CCAGCCGCCG CCGCCACCAC CTCCTCCTCC ACCTCCATCC
    AAGAAAAAAG GACAAAAGCA AGGAAGGATG GAGACTTCTG CAGAAAAACC AATCATACCT
    GCAGAACCCC CTGAGCCTGT CCTCTGTGGC AGCATGGCCA TAGGGGCTGT GTCACTGGCT
    GTTGCCAAAG CCAGTGCTGT ACTGGACAAT AATCCTCTCT ACCTGAACAT TGCATTACTG
    AAGCACCACC AGGAACAGCC AAAAAAGCTA ACTATACCTC TTCTGATGGT GTCGCTGGTC
    AGCTGTGGGA AGTCATCACC TGGGAAGCTG AATTTGATGA AAGAAGTGAT CTGTATACCC
    CATCCTGGAT TAACAGCTAA ACAAGGTGTA GAGATGCTTC TGGAAATTCA GAAACACATC
    AACAAAGCAA TTGAAATGTC CCCTCCTCCA AAGCCAGAGA CAAAAAAAAG TCACGATGGA
    GGCAAAAGAG GCCAACAGCA GATCACCGGC AAGATGTCCC ATCTTGGCTG TTTAACCATT
    AATTACGACA CTATAGAGCA GCCACTGCTC CTCATACAAG GAATCTGTGC AAACCTGGGG
    CTAGAATTGG GAACGAACCT GCATCTAGCC ATCAACTGTG CCGCACATGA GCTGATGGAC
    TACAGTAAAG GAAAGTATGA AGTGATGATG GGGACCTACA AAAATGCAGC TGAGATGGTT
    GACCTGTATG TGGATCTGAT CAACAAGTTC CCTTCAATTA TTGCGTTAAT TGATCCTTTC
    AGGAAGGAGG ACTCTGAACA GTGGGACAGC ATCTATAATG CTCTTGGTTC CAGGTGTTAC
    ATAATTGCAG GCACCGCGTC CAAAAGCATC TCTAAACTGC TAGAGGAGGG CAACATCAGC
    ATCCTCAAAT ACAGCGGGCT GATCATAAAA CACATAAACC AAACTACGAT GTCGGACTTG
    GTGGAAATAA CCAATCTCAT TGACGGTAAG AAGCTCATCG CTGTCTTTGG AAGCACAGAA
    GGAGAATCGT CTGATGACAG CCTTGTCGAT TTGGCTGTTG GACTTGGTGT CCGGTTTATC
    AAGTTGGGAG GTCTTTCTCG TGGCGAACGG GTGACCAAGT ACAACCGCCT CTTTACTATA
    GAGGAAGAAC TTGGCCAGAA TGGAACACTG GGCTTCAATG AAGAACACAC ATTTTTCTCC
    TGGAATGAGG AAGCTGAAGA GGCTGGTGAG CTGCATGAGG TCCCTGAGCC CATCTTCCCC
    ACAGAAGTTG CGGAGGATCT CGCCAAACTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841596.1
    CDS584..2296
    Translation

    Target ORF information:

    RefSeq Version XM_024985828.1
    Organism Bos taurus(cattle)
    Definition Bos taurus enolase 4 (ENO4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985828.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGGGGAAG AAGGCGGCGG CCGCAGCTGT GGGAGCACTA GGGAGCTGCA GAAGCTGAAG 
    CAGCAGGCGA TGGAGTATTA CCGCGAGAAC GACGTTCCGC GCAGGCTGGA AGAACTGCTC
    AACTCCACCT TCTACCTCCA GCCTGCCGAC GTCTACGGGC ACCTGAACAT ATGTTCTGTG
    GTGATCGCAA CTCACTTTGA AGCCCACGAG AACGCTTCAC CTGAGCTCGT TGAAGCCGAG
    GAAGTGGAAA GGTTGGATGC TGTCAGCACC GCAGTGCAGT GGGTCAACGA AACTCTCACT
    GAGGAGCTCC AGGGCCTAGT GCCCTCCAGC CAGGCTGAGG TGGATCAGGT GCTCAGGACA
    TTCTTTGAAA ATAAAGTACA AGAAGATAAG GAGAGAAAAG AATTGGAAAA GAACCTAGAA
    GACTCAACAG TGCTTTCACC CCAGCCGCCG CCGCCACCAC CTCCTCCTCC ACCTCCATCC
    AAGAAAAAAG GACAAAAGCA AGGAAGGATG GAGACTTCTG CAGAAAAACC AATCATACCT
    GCAGAACCCC CTGAGCCTGT CCTCTGTGGC AGCATGGCCA TAGGGGCTGT GTCACTGGCT
    GTTGCCAAAG CCAGTGCTGT ACTGGACAAT AATCCTCTCT ACCTGAACAT TGCATTACTG
    AAGCACCACC AGGAACAGCC AAAAAAGCTA ACTATACCTC TTCTGATGGT GTCGCTGGTC
    AGCTGTGGGA AGTCATCACC TGGGAAGCTG AATTTGATGA AAGAAGTGAT CTGTATACCC
    CATCCTGGAT TAACAGCTAA ACAAGGTGTA GAGATGCTTC TGGAAATTCA GAAACACATC
    AACAAAGCAA TTGAAATGTC CCCTCCTCCA AAGCCAGAGA CAAAAAAAAG TCACGATGGA
    GGCAAAAGAG GCCAACAGCA GATCACCGGC AAGATGTCCC ATCTTGGCTG TTTAACCATT
    AATTACGACA CTATAGAGCA GCCACTGCTC CTCATACAAG GAATCTGTGC AAACCTGGGG
    CTAGAATTGG GAACGAACCT GCATCTAGCC ATCAACTGTG CCGCACATGA GCTGATGGAC
    TACAGTAAAG GAAAGTATGA AGTGATGATG GGGACCTACA AAAATGCAGC TGAGATGGTT
    GACCTGTATG TGGATCTGAT CAACAAGTTC CCTTCAATTA TTGCGTTAAT TGATCCTTTC
    AGGAAGGAGG ACTCTGAACA GTGGGACAGC ATCTATAATG CTCTTGGTTC CAGGTGTTAC
    ATAATTGCAG GCACCGCGTC CAAAAGCATC TCTAAACTGC TAGAGGAGGG CAACATCAGC
    ATCCTCAAAT ACAGCGGGCT GATCATAAAA CACATAAACC AAACTACGAT GTCGGACTTG
    GTGGAAATAA CCAATCTCAT TGACGGTAAG AAGCTCATCG CTGTCTTTGG AAGCACAGAA
    GGAGAATCGT CTGATGACAG CCTTGTCGAT TTGGCTGTTG GACTTGGTGT CCGGTTTATC
    AAGTTGGGAG GTCTTTCTCG TGGCGAACGG GTGACCAAGT ACAACCGCCT CTTTACTATA
    GAGGAAGAAC TTGGCCAGAA TGGAACACTG GGCTTCAATG AAGAACACAC ATTTTTCTCC
    TGGAATGAGG AAGCTGAAGA GGCTGGTGAG CTGCATGAGG TCCCTGAGCC CATCTTCCCC
    ACAGAAGTTG CGGAGGATCT CGCCAAACTT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.