NOL8 cDNA ORF clone, Bos taurus(cattle)

The following NOL8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOL8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238972 NM_001191213.2
Latest version!
Bos taurus nucleolar protein 8 (NOL8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa237276 XM_024995970.1
Latest version!
Bos taurus nucleolar protein 8 (NOL8), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa237277 XM_024995971.1
Latest version!
Bos taurus nucleolar protein 8 (NOL8), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa237278 XM_024995972.1
Latest version!
Bos taurus nucleolar protein 8 (NOL8), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa37200 NM_001191213.1
Latest version!
Bos taurus nucleolar protein 8 (NOL8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238972
    Clone ID Related Accession (Same CDS sequence) NM_001191213.2
    Accession Version NM_001191213.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product nucleolar protein 8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000008.1. On May 29, 2018 this sequence version replaced NM_001191213.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGAGTCCATG
    GGTAGAGCCT CTGGGAAGCT CTTTGACAGC AGCGATGATG AGGAATCTTG TGCTGAAGAT
    GACAATAACA GGTTCAGAAT TAAACCTCAG TTTGAGGGCA GAGCTGGACA GAAGCTCATG
    AATTTGCAGT CTCACTTTGG CACTGATGAC AGATTTCGCA TGGACTCCCG ATTTCTAGAA
    AGTGATAGTG AAGATGATCA TGAAGAAATA AATGAAAAGA AAACTCCTGA GGAGGAAGAG
    CTTGCTGCAG AAAAATTGAA AGCCCTGAAT GTTGTGCAGA GTGTTTTGCA GATCAGCTTA
    AGCAATTCTA CAAGCAAAGG ATCAGTAGCT GCTAAGAAAT TTAAGGACAT CATACATTAT
    GATCCAACAA GGGATGACCA TGCAACTTAC GAAAGAAAGC AGGATGATAA ACCAAAAGAA
    AGTAAAGCAA AACGAAAGAA GAAAAGGGAG GAAGCTGAGA AACTACCTGA GGTGTCTAAA
    GAAATGTATT ATAACATTGC TACGGATTTG AAACAAATAT TCCAAAGTGT AAAAGATACT
    AGTGAAAAGG AAGATAACAC ACCTTGGAAT GAGGACTCTG ATGGAGAGAA AGCTGAGGTA
    GTCCAGGACA CTCCAGCTCT GATGACTCGC AGTGAGCAAA CCGGCGGATT CACATTTTCC
    TTTTTTGAGT CTGATGCCAA AGATGAAAAA GAAGAGACCT ATAGAGTTGA AGCAGTAAAA
    CCTGGGAAGG TTGCCTGGCA GGGAGACCCT CGTTTCCAAG ACAGCAGTTC AGAAGAGGAA
    GATGTTACTG AAGAAACAGA TGACAAAAAG CAGAGCCCTG AAGAAGTACC ATTACCTGAG
    AAAGAGACTA CTAGATTTTT CTTTTTCTCT AAGAATGATG AGAGACTTCA TGGTTCTGAC
    TTATTTTGGA GAGGAGTGGG AAGTAATATT AGCAGGAATT CTTGGGAGAT CAGAACAAAC
    AATTTGCGAA TGGACTGTCG GAAGAAACAT AAAGATGCCA AAAGGAGATT GAAGCTAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178142.2
    CDS325..3807
    Translation

    Target ORF information:

    RefSeq Version NM_001191213.2
    Organism Bos taurus(cattle)
    Definition Bos taurus nucleolar protein 8 (NOL8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191213.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGAGTCCATG
    GGTAGAGCCT CTGGGAAGCT CTTTGACAGC AGCGATGATG AGGAATCTTG TGCTGAAGAT
    GACAATAACA GGTTCAGAAT TAAACCTCAG TTTGAGGGCA GAGCTGGACA GAAGCTCATG
    AATTTGCAGT CTCACTTTGG CACTGATGAC AGATTTCGCA TGGACTCCCG ATTTCTAGAA
    AGTGATAGTG AAGATGATCA TGAAGAAATA AATGAAAAGA AAACTCCTGA GGAGGAAGAG
    CTTGCTGCAG AAAAATTGAA AGCCCTGAAT GTTGTGCAGA GTGTTTTGCA GATCAGCTTA
    AGCAATTCTA CAAGCAAAGG ATCAGTAGCT GCTAAGAAAT TTAAGGACAT CATACATTAT
    GATCCAACAA GGGATGACCA TGCAACTTAC GAAAGAAAGC AGGATGATAA ACCAAAAGAA
    AGTAAAGCAA AACGAAAGAA GAAAAGGGAG GAAGCTGAGA AACTACCTGA GGTGTCTAAA
    GAAATGTATT ATAACATTGC TACGGATTTG AAACAAATAT TCCAAAGTGT AAAAGATACT
    AGTGAAAAGG AAGATAACAC ACCTTGGAAT GAGGACTCTG ATGGAGAGAA AGCTGAGGTA
    GTCCAGGACA CTCCAGCTCT GATGACTCGC AGTGAGCAAA CCGGCGGATT CACATTTTCC
    TTTTTTGAGT CTGATGCCAA AGATGAAAAA GAAGAGACCT ATAGAGTTGA AGCAGTAAAA
    CCTGGGAAGG TTGCCTGGCA GGGAGACCCT CGTTTCCAAG ACAGCAGTTC AGAAGAGGAA
    GATGTTACTG AAGAAACAGA TGACAAAAAG CAGAGCCCTG AAGAAGTACC ATTACCTGAG
    AAAGAGACTA CTAGATTTTT CTTTTTCTCT AAGAATGATG AGAGACTTCA TGGTTCTGAC
    TTATTTTGGA GAGGAGTGGG AAGTAATATT AGCAGGAATT CTTGGGAGAT CAGAACAAAC
    AATTTGCGAA TGGACTGTCG GAAGAAACAT AAAGATGCCA AAAGGAGATT GAAGCTAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237276
    Clone ID Related Accession (Same CDS sequence) XM_024995970.1
    Accession Version XM_024995970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nucleolar protein 8 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGTAAGAAAT
    CAGGAGTCCA TGGGTAGAGC CTCTGGGAAG CTCTTTGACA GCAGCGATGA TGAGGAATCT
    TGTGCTGAAG ATGACAATAA CAGGTTCAGA ATTAAACCTC AGTTTGAGGG CAGAGCTGGA
    CAGAAGCTCA TGAATTTGCA GTCTCACTTT GGCACTGATG ACAGATTTCG CATGGACTCC
    CGATTTCTAG AAAGTGATAG TGAAGATGAT CATGAAGAAA TAAATGAAAA GAAAACTCCT
    GAGGAGGAAG AGCTTGCTGC AGAAAAATTG AAAGCCCTGA ATGTTGTGCA GAGTGTTTTG
    CAGATCAGCT TAAGCAATTC TACAAGCAAA GGATCAGTAG CTGCTAAGAA ATTTAAGGAC
    ATCATACATT ATGATCCAAC AAGGGATGAC CATGCAACTT ACGAAAGAAA GCAGGATGAT
    AAACCAAAAG AAAGTAAAGC AAAACGAAAG AAGAAAAGGG AGGAAGCTGA GAAACTACCT
    GAGGTGTCTA AAGAAATGTA TTATAACATT GCTACGGATT TGAAACAAAT ATTCCAAAGT
    GTAAAAGATA CTAGTGAAAA GGAAGATAAC ACACCTTGGA ATGAGGACTC TGATGGAGAG
    AAAGCTGAGG TAGTCCAGGA CACTCCAGCT CTGATGACTC GCAGTGAGCA AACCGGCGGA
    TTCACATTTT CCTTTTTTGA GTCTGATGCC AAAGATGAAA AAGAAGAGAC CTATAGAGTT
    GAAGCAGTAA AACCTGGGAA GGTTGCCTGG CAGGGAGACC CTCGTTTCCA AGACAGCAGT
    TCAGAAGAGG AAGATGTTAC TGAAGAAACA GATGACAAAA AGCAGAGCCC TGAAGAAGTA
    CCATTACCTG AGAAAGAGAC TACTAGATTT TTCTTTTTCT CTAAGAATGA TGAGAGACTT
    CATGGTTCTG ACTTATTTTG GAGAGGAGTG GGAAGTAATA TTAGCAGGAA TTCTTGGGAG
    ATCAGAACAA ACAATTTGCG AATGGACTGT CGGAAGAAAC ATAAAGATGC CAAAAGGAGA
    TTGAAGCTAA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851738.1
    CDS340..3834
    Translation

    Target ORF information:

    RefSeq Version XM_024995970.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nucleolar protein 8 (NOL8), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGTAAGAAAT
    CAGGAGTCCA TGGGTAGAGC CTCTGGGAAG CTCTTTGACA GCAGCGATGA TGAGGAATCT
    TGTGCTGAAG ATGACAATAA CAGGTTCAGA ATTAAACCTC AGTTTGAGGG CAGAGCTGGA
    CAGAAGCTCA TGAATTTGCA GTCTCACTTT GGCACTGATG ACAGATTTCG CATGGACTCC
    CGATTTCTAG AAAGTGATAG TGAAGATGAT CATGAAGAAA TAAATGAAAA GAAAACTCCT
    GAGGAGGAAG AGCTTGCTGC AGAAAAATTG AAAGCCCTGA ATGTTGTGCA GAGTGTTTTG
    CAGATCAGCT TAAGCAATTC TACAAGCAAA GGATCAGTAG CTGCTAAGAA ATTTAAGGAC
    ATCATACATT ATGATCCAAC AAGGGATGAC CATGCAACTT ACGAAAGAAA GCAGGATGAT
    AAACCAAAAG AAAGTAAAGC AAAACGAAAG AAGAAAAGGG AGGAAGCTGA GAAACTACCT
    GAGGTGTCTA AAGAAATGTA TTATAACATT GCTACGGATT TGAAACAAAT ATTCCAAAGT
    GTAAAAGATA CTAGTGAAAA GGAAGATAAC ACACCTTGGA ATGAGGACTC TGATGGAGAG
    AAAGCTGAGG TAGTCCAGGA CACTCCAGCT CTGATGACTC GCAGTGAGCA AACCGGCGGA
    TTCACATTTT CCTTTTTTGA GTCTGATGCC AAAGATGAAA AAGAAGAGAC CTATAGAGTT
    GAAGCAGTAA AACCTGGGAA GGTTGCCTGG CAGGGAGACC CTCGTTTCCA AGACAGCAGT
    TCAGAAGAGG AAGATGTTAC TGAAGAAACA GATGACAAAA AGCAGAGCCC TGAAGAAGTA
    CCATTACCTG AGAAAGAGAC TACTAGATTT TTCTTTTTCT CTAAGAATGA TGAGAGACTT
    CATGGTTCTG ACTTATTTTG GAGAGGAGTG GGAAGTAATA TTAGCAGGAA TTCTTGGGAG
    ATCAGAACAA ACAATTTGCG AATGGACTGT CGGAAGAAAC ATAAAGATGC CAAAAGGAGA
    TTGAAGCTAA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237277
    Clone ID Related Accession (Same CDS sequence) XM_024995971.1
    Accession Version XM_024995971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nucleolar protein 8 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGTAAGAAAT
    GAGTCCATGG GTAGAGCCTC TGGGAAGCTC TTTGACAGCA GCGATGATGA GGAATCTTGT
    GCTGAAGATG ACAATAACAG GTTCAGAATT AAACCTCAGT TTGAGGGCAG AGCTGGACAG
    AAGCTCATGA ATTTGCAGTC TCACTTTGGC ACTGATGACA GATTTCGCAT GGACTCCCGA
    TTTCTAGAAA GTGATAGTGA AGATGATCAT GAAGAAATAA ATGAAAAGAA AACTCCTGAG
    GAGGAAGAGC TTGCTGCAGA AAAATTGAAA GCCCTGAATG TTGTGCAGAG TGTTTTGCAG
    ATCAGCTTAA GCAATTCTAC AAGCAAAGGA TCAGTAGCTG CTAAGAAATT TAAGGACATC
    ATACATTATG ATCCAACAAG GGATGACCAT GCAACTTACG AAAGAAAGCA GGATGATAAA
    CCAAAAGAAA GTAAAGCAAA ACGAAAGAAG AAAAGGGAGG AAGCTGAGAA ACTACCTGAG
    GTGTCTAAAG AAATGTATTA TAACATTGCT ACGGATTTGA AACAAATATT CCAAAGTGTA
    AAAGATACTA GTGAAAAGGA AGATAACACA CCTTGGAATG AGGACTCTGA TGGAGAGAAA
    GCTGAGGTAG TCCAGGACAC TCCAGCTCTG ATGACTCGCA GTGAGCAAAC CGGCGGATTC
    ACATTTTCCT TTTTTGAGTC TGATGCCAAA GATGAAAAAG AAGAGACCTA TAGAGTTGAA
    GCAGTAAAAC CTGGGAAGGT TGCCTGGCAG GGAGACCCTC GTTTCCAAGA CAGCAGTTCA
    GAAGAGGAAG ATGTTACTGA AGAAACAGAT GACAAAAAGC AGAGCCCTGA AGAAGTACCA
    TTACCTGAGA AAGAGACTAC TAGATTTTTC TTTTTCTCTA AGAATGATGA GAGACTTCAT
    GGTTCTGACT TATTTTGGAG AGGAGTGGGA AGTAATATTA GCAGGAATTC TTGGGAGATC
    AGAACAAACA ATTTGCGAAT GGACTGTCGG AAGAAACATA AAGATGCCAA AAGGAGATTG
    AAGCTAAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851739.1
    CDS340..3831
    Translation

    Target ORF information:

    RefSeq Version XM_024995971.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nucleolar protein 8 (NOL8), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGTAAGAAAT
    GAGTCCATGG GTAGAGCCTC TGGGAAGCTC TTTGACAGCA GCGATGATGA GGAATCTTGT
    GCTGAAGATG ACAATAACAG GTTCAGAATT AAACCTCAGT TTGAGGGCAG AGCTGGACAG
    AAGCTCATGA ATTTGCAGTC TCACTTTGGC ACTGATGACA GATTTCGCAT GGACTCCCGA
    TTTCTAGAAA GTGATAGTGA AGATGATCAT GAAGAAATAA ATGAAAAGAA AACTCCTGAG
    GAGGAAGAGC TTGCTGCAGA AAAATTGAAA GCCCTGAATG TTGTGCAGAG TGTTTTGCAG
    ATCAGCTTAA GCAATTCTAC AAGCAAAGGA TCAGTAGCTG CTAAGAAATT TAAGGACATC
    ATACATTATG ATCCAACAAG GGATGACCAT GCAACTTACG AAAGAAAGCA GGATGATAAA
    CCAAAAGAAA GTAAAGCAAA ACGAAAGAAG AAAAGGGAGG AAGCTGAGAA ACTACCTGAG
    GTGTCTAAAG AAATGTATTA TAACATTGCT ACGGATTTGA AACAAATATT CCAAAGTGTA
    AAAGATACTA GTGAAAAGGA AGATAACACA CCTTGGAATG AGGACTCTGA TGGAGAGAAA
    GCTGAGGTAG TCCAGGACAC TCCAGCTCTG ATGACTCGCA GTGAGCAAAC CGGCGGATTC
    ACATTTTCCT TTTTTGAGTC TGATGCCAAA GATGAAAAAG AAGAGACCTA TAGAGTTGAA
    GCAGTAAAAC CTGGGAAGGT TGCCTGGCAG GGAGACCCTC GTTTCCAAGA CAGCAGTTCA
    GAAGAGGAAG ATGTTACTGA AGAAACAGAT GACAAAAAGC AGAGCCCTGA AGAAGTACCA
    TTACCTGAGA AAGAGACTAC TAGATTTTTC TTTTTCTCTA AGAATGATGA GAGACTTCAT
    GGTTCTGACT TATTTTGGAG AGGAGTGGGA AGTAATATTA GCAGGAATTC TTGGGAGATC
    AGAACAAACA ATTTGCGAAT GGACTGTCGG AAGAAACATA AAGATGCCAA AAGGAGATTG
    AAGCTAAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237278
    Clone ID Related Accession (Same CDS sequence) XM_024995972.1
    Accession Version XM_024995972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nucleolar protein 8 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA ACAGGAGTCC
    ATGGGTAGAG CCTCTGGGAA GCTCTTTGAC AGCAGCGATG ATGAGGAATC TTGTGCTGAA
    GATGACAATA ACAGGTTCAG AATTAAACCT CAGTTTGAGG GCAGAGCTGG ACAGAAGCTC
    ATGAATTTGC AGTCTCACTT TGGCACTGAT GACAGATTTC GCATGGACTC CCGATTTCTA
    GAAAGTGATA GTGAAGATGA TCATGAAGAA ATAAATGAAA AGAAAACTCC TGAGGAGGAA
    GAGCTTGCTG CAGAAAAATT GAAAGCCCTG AATGTTGTGC AGAGTGTTTT GCAGATCAGC
    TTAAGCAATT CTACAAGCAA AGGATCAGTA GCTGCTAAGA AATTTAAGGA CATCATACAT
    TATGATCCAA CAAGGGATGA CCATGCAACT TACGAAAGAA AGCAGGATGA TAAACCAAAA
    GAAAGTAAAG CAAAACGAAA GAAGAAAAGG GAGGAAGCTG AGAAACTACC TGAGGTGTCT
    AAAGAAATGT ATTATAACAT TGCTACGGAT TTGAAACAAA TATTCCAAAG TGTAAAAGAT
    ACTAGTGAAA AGGAAGATAA CACACCTTGG AATGAGGACT CTGATGGAGA GAAAGCTGAG
    GTAGTCCAGG ACACTCCAGC TCTGATGACT CGCAGTGAGC AAACCGGCGG ATTCACATTT
    TCCTTTTTTG AGTCTGATGC CAAAGATGAA AAAGAAGAGA CCTATAGAGT TGAAGCAGTA
    AAACCTGGGA AGGTTGCCTG GCAGGGAGAC CCTCGTTTCC AAGACAGCAG TTCAGAAGAG
    GAAGATGTTA CTGAAGAAAC AGATGACAAA AAGCAGAGCC CTGAAGAAGT ACCATTACCT
    GAGAAAGAGA CTACTAGATT TTTCTTTTTC TCTAAGAATG ATGAGAGACT TCATGGTTCT
    GACTTATTTT GGAGAGGAGT GGGAAGTAAT ATTAGCAGGA ATTCTTGGGA GATCAGAACA
    AACAATTTGC GAATGGACTG TCGGAAGAAA CATAAAGATG CCAAAAGGAG ATTGAAGCTA
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851740.1
    CDS340..3825
    Translation

    Target ORF information:

    RefSeq Version XM_024995972.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nucleolar protein 8 (NOL8), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGT
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA ACAGGAGTCC
    ATGGGTAGAG CCTCTGGGAA GCTCTTTGAC AGCAGCGATG ATGAGGAATC TTGTGCTGAA
    GATGACAATA ACAGGTTCAG AATTAAACCT CAGTTTGAGG GCAGAGCTGG ACAGAAGCTC
    ATGAATTTGC AGTCTCACTT TGGCACTGAT GACAGATTTC GCATGGACTC CCGATTTCTA
    GAAAGTGATA GTGAAGATGA TCATGAAGAA ATAAATGAAA AGAAAACTCC TGAGGAGGAA
    GAGCTTGCTG CAGAAAAATT GAAAGCCCTG AATGTTGTGC AGAGTGTTTT GCAGATCAGC
    TTAAGCAATT CTACAAGCAA AGGATCAGTA GCTGCTAAGA AATTTAAGGA CATCATACAT
    TATGATCCAA CAAGGGATGA CCATGCAACT TACGAAAGAA AGCAGGATGA TAAACCAAAA
    GAAAGTAAAG CAAAACGAAA GAAGAAAAGG GAGGAAGCTG AGAAACTACC TGAGGTGTCT
    AAAGAAATGT ATTATAACAT TGCTACGGAT TTGAAACAAA TATTCCAAAG TGTAAAAGAT
    ACTAGTGAAA AGGAAGATAA CACACCTTGG AATGAGGACT CTGATGGAGA GAAAGCTGAG
    GTAGTCCAGG ACACTCCAGC TCTGATGACT CGCAGTGAGC AAACCGGCGG ATTCACATTT
    TCCTTTTTTG AGTCTGATGC CAAAGATGAA AAAGAAGAGA CCTATAGAGT TGAAGCAGTA
    AAACCTGGGA AGGTTGCCTG GCAGGGAGAC CCTCGTTTCC AAGACAGCAG TTCAGAAGAG
    GAAGATGTTA CTGAAGAAAC AGATGACAAA AAGCAGAGCC CTGAAGAAGT ACCATTACCT
    GAGAAAGAGA CTACTAGATT TTTCTTTTTC TCTAAGAATG ATGAGAGACT TCATGGTTCT
    GACTTATTTT GGAGAGGAGT GGGAAGTAAT ATTAGCAGGA ATTCTTGGGA GATCAGAACA
    AACAATTTGC GAATGGACTG TCGGAAGAAA CATAAAGATG CCAAAAGGAG ATTGAAGCTA
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37200
    Clone ID Related Accession (Same CDS sequence) NM_001191213.1
    Accession Version NM_001191213.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product nucleolar protein 8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02024046.1. On Jul 17, 2010 this sequence version replaced XM_001253324.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGA
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGAGTCCATG
    GGTAGAGCCT CTGGGAAGCT CTTTGACAGC AGCGATGATG AGGAATCTTG TGCTGAAGAT
    GACAATAACA GGTTCAGAAT TAAACCTCAG TTTGAGGGCA GAGCTGGACA GAAGCTCATG
    AATTTGCAGT CTCACTTTGG CACTGATGAC AGATTTCGCA TGGACTCCCG ATTTCTAGAA
    AGTGATAGTG AAGATGATCA TGAAGAAATA AATGAAAAGA AAACTCCTGA GGAGGAAGAG
    CTTGCTGCAG AAAAATTGAA AGCCCTGAAT GTTGTGCAGA GTGTTTTGCA GATCAGCTTA
    AGCAATTCTA CAAGCAAAGG ATCAGTAGCT GCTAAGAAAT TTAAGGACAT CATACATTAT
    GATCCAACAA GGGATGACCA TGCAACTTAC GAAAGAAAGC AGGATGATAA ACCAAAAGAA
    AGTAAAGCAA AACGAAAGAA GAAAAGGGAG GAAGCTGAGA AACTACCTGA GGTGTCTAAA
    GAAATGTATT ATAACATTGC TACGGATTTG AAACAAATAT TCCAAAGTGT AAAAGATACT
    AGTGAAAAGG AAGATAACAC ACCTTGGAAT GAGGACTCTG ATGGAGAGAA AGCTGAGGTA
    GTCCAGGACA CTCCAGCTCT GATGACTCGC AGTGAGCAAA CCGGCGGATT CACATTTTCC
    TTTTTTGAGT CTGATGCCAA AGATGAAAAA GAAGAGACCT ATAGAGTTGA AGCAGTAAAA
    CCTGGGAAGG TTGCCTGGCA GGGAGACCCT CGTTTCCAAG ACAGCAGTTC AGAAGAGGAA
    GATGTTACTG AAGAAACAGA TGACAAAAAG CAGAGCCCTG AAGAAGTACC ATTACCTGAG
    AAAGAGACTA CTAGATTTTT CTTTTTCTCT AAGAATGATG AGAGACTTCA TGGTTCTGAC
    TTATTTTGGA GAGGAGTGGG AAGTAATATT AGCAGGAATT CTTGGGAGAT CAGAACAAAC
    AATTTGCGAA TGGACTGTCG GAAGAAACAT AAAGATGCCA AAAGGAGATT GAAGCTAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178142.1
    CDS325..3807
    Translation

    Target ORF information:

    RefSeq Version NM_001191213.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nucleolar protein 8 (NOL8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191213.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAGCCA ACAGAGAAAT GAAACGCCTT TTTGTGGGTG GTCTTGGGCA GAATATTTCT 
    GAGGCAGACC TGCAGAATCA GTTCGGCAGA TTTGGAGAAG TTTCTGATGT GGAGATCATC
    ACTCGGAAAG ATGATCAAGG AAACCCACAG AAAGTTTTTG CATATATCAA CATCAGAGTA
    ACAGAAGCAG ACCTGAAGAA ATGTATGTCT GTTTTAAATA AAACAAAATG GAAAGGTGGG
    ACACTGCAAA TTCAGCTAGC AAAGGAAAGT TTTTTGCACA GATTGGCCCA AGAGAGAGAA
    GAAGCAAAAG TAAAGAAAGG AAAATCAACA ACAGGCAACA CCAGCTTGTT AGAAAAGATG
    AAAACGGTGG ATTTCCATGT GAAAGCTGTG CCAGGAACAG AAGTACCAGG GCACAAAAAT
    TGGGTTGTGA GTAAATTTGG AAGAGTCTTA CCTGTCCTTC ATCTAAAGAA TGAACGTAAA
    CGTAAAATCA TAAAGTATGA TCCCTCAAAA TACTGCCACA ACTTAAAGAA GATAGGGGAG
    GATTTCACAA ATGCTGTCCC TATATCCAAC CTCACCTGGG AACTGGAAGG AGGGAATGAT
    CCTATGAGTA AGAAACGGCG AGGAGAATTC TCTGACTTTC ACAGCCCTGC CAAAAAGATA
    ATAAAAGTAC AGAAGAATGA GGATTCTACT GCATTTCCTA CCATCAGATC AAAACCTAGA
    GGCATAGTGG AGAGTCCACA TTTAATACAA CAGCTTGCAC AAAAAGCACC TCATAATTCC
    AGTACTCCTA AATCACCGTA TATAACTGAT TTTGATCATC AGAAATTTAA AAATGTATTT
    TTTCAGACTT CTGGTTTAGA AACTACCAAA AAGAGAAATA GCATATCTGA TGATGATATT
    GATTCTGAAG ATGAATTAAG GCTAATGATT GCACGAGAGG AAAACTTAGA GAAAACTACA
    TGGTCCTCAA ATGAATTTGA AAATGATCCC TTTGAAGTTG TAAGGGATGA TTTTAAATCT
    GATTTTCACA AATTTCATAC TTCAACAGGT TTAGGCCTCA AAAACAGTGA AAATAATGTT
    ACGGAAAATG GTTGTGACTC TGATTCAGGA GATACAGATG AAATCATTGC AATGAAAAAA
    AGTTCTGGTA AGATCGAAAA CGGTGTAGAT TTTTCACAAG GAGAAAAGTC TATGTACAAG
    AAGACTTTGA AAAGCAGAAA AAAGTATGAT CTTTCTGATG ACTGTATTAA AGAACAAAAA
    AGAAAAAACA AAGTAGAGAT AGCCCTCAGT CACAGAGTTA AGCCTCTTAG TTGTAAATCT
    CTGATTGAGT CCAGTAGCAG TGAAGATGCT GATTCTGTAT CAGAATCAAC TGAGTGTGAA
    GGAGATAAGG AGTATAACAC CTTGATGGAA AACTGTCTCC GTGTGAATCT GACTTTGGCT
    GATTTGGAAC AGCTGGCTGG GGGTAACCAG GAGGCTCCAA AAGAAAAGAC TGAGAGCAGT
    GGCCAGGAAA CCACTGCCAA GTGTGACAGG GCCTCCAAGA GCCGAAGGAC TCCAGGTGAC
    CTCCGCAGAG GCCAGCAGTG TATTCATCCT GAGGAAATTG TGGCTTCTCT TTTAGAAGGA
    AGAGAGAACC CATGTGGAAA ACAGAAAACA AAGGAAAATA CCTTAAAGCC AAAATTTCAG
    GCTTTCAAAG GAGTGGGCTG TCTCTATGGA AAGGAATCAG TGAAAAAATC CTTGCAAGAG
    AGTGTTGCCT CTAATAATAT TAATAAAGAT CAAATTTCCT TGAAACTTGA GGAACCGAGG
    AACGTGTCCA TGGGAAAATG GTCCCTGTGT GCTAATGGCC TATCAAGTGA ACAGACTCCT
    TTCCAACATG CAAAGGAGGC AAATGACTCA AACCAAATTC AGCCTCAAAA GAGACAGTCT
    TTTTTGAGCC AGGGTCACAA AGTGGTGTCC CCTAACAGTT CAGAAAAGAG AAATAGAAAT
    CCTATTTCTA GTCTGTTGCC ACTGAAAGAT AAGAAATCTT CAAGTTGTGG GGCTAAGACT
    CCCAACAGAG GCCTTGATGA AGACTGTTGC CACGGGACTG GAAAGTCAGG AGAGAGCTCT
    GAAGGGAGGC CTGATCTGTG CAGCTCCAAG ACCCCTGAGA AATCACCAGA GGTTTTCTCA
    AGGAGAGACT CCCAGGGAAG CAAAACTGAC TTCCCACTTT CTGTTAATAG TTCATCAGAT
    GTTAACGCTA AAGATAAGCA TGCTGAAGAT AATCAGAAAC GTTTGGCAGC CTTAGAGGCA
    AGGCAGAAAG CCAAAGAAGT CCAAAAGAAG TTGGTTCACA ATGCTCTGGC AAATTTGGAT
    GGTCATCCAG AGGACAAGCC GACGCACATC ATCTTTGGTT CCAATAGTGA AAGCGAAACA
    GAGGAGACAT CCACTCAGGA GCAAAGCCAT CCAGGAGAGG AACTGGTAAA AGAGTCCATG
    GGTAGAGCCT CTGGGAAGCT CTTTGACAGC AGCGATGATG AGGAATCTTG TGCTGAAGAT
    GACAATAACA GGTTCAGAAT TAAACCTCAG TTTGAGGGCA GAGCTGGACA GAAGCTCATG
    AATTTGCAGT CTCACTTTGG CACTGATGAC AGATTTCGCA TGGACTCCCG ATTTCTAGAA
    AGTGATAGTG AAGATGATCA TGAAGAAATA AATGAAAAGA AAACTCCTGA GGAGGAAGAG
    CTTGCTGCAG AAAAATTGAA AGCCCTGAAT GTTGTGCAGA GTGTTTTGCA GATCAGCTTA
    AGCAATTCTA CAAGCAAAGG ATCAGTAGCT GCTAAGAAAT TTAAGGACAT CATACATTAT
    GATCCAACAA GGGATGACCA TGCAACTTAC GAAAGAAAGC AGGATGATAA ACCAAAAGAA
    AGTAAAGCAA AACGAAAGAA GAAAAGGGAG GAAGCTGAGA AACTACCTGA GGTGTCTAAA
    GAAATGTATT ATAACATTGC TACGGATTTG AAACAAATAT TCCAAAGTGT AAAAGATACT
    AGTGAAAAGG AAGATAACAC ACCTTGGAAT GAGGACTCTG ATGGAGAGAA AGCTGAGGTA
    GTCCAGGACA CTCCAGCTCT GATGACTCGC AGTGAGCAAA CCGGCGGATT CACATTTTCC
    TTTTTTGAGT CTGATGCCAA AGATGAAAAA GAAGAGACCT ATAGAGTTGA AGCAGTAAAA
    CCTGGGAAGG TTGCCTGGCA GGGAGACCCT CGTTTCCAAG ACAGCAGTTC AGAAGAGGAA
    GATGTTACTG AAGAAACAGA TGACAAAAAG CAGAGCCCTG AAGAAGTACC ATTACCTGAG
    AAAGAGACTA CTAGATTTTT CTTTTTCTCT AAGAATGATG AGAGACTTCA TGGTTCTGAC
    TTATTTTGGA GAGGAGTGGG AAGTAATATT AGCAGGAATT CTTGGGAGAT CAGAACAAAC
    AATTTGCGAA TGGACTGTCG GAAGAAACAT AAAGATGCCA AAAGGAGATT GAAGCTAAAA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.