PNPLA6 cDNA ORF clone, Bos taurus(cattle)

The following PNPLA6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PNPLA6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236295 XM_024994643.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236295 XM_024994642.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236296 XM_024994644.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236297 XM_024994645.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236298 XM_024994647.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236298 XM_024994646.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236299 XM_024994648.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236300 XM_024994651.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236300 XM_024994649.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236300 XM_024994654.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236300 XM_024994650.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236301 XM_024994652.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236302 XM_024994655.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa38278 NM_001076547.3
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa38278 XM_024994653.1
Latest version!
Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa38278 NM_001076547.2 Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236295
    Clone ID Related Accession (Same CDS sequence) XM_024994642.1 , XM_024994643.1
    Accession Version XM_024994642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGGCGTGGGG GGCCACCCCT TGTCCCCACA GGAGGAGGAG
    AAGTCGATCC TCCGGCACCG GCGCTGGCTG CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT
    ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850410.1
    CDS140..4285
    Translation

    Target ORF information:

    RefSeq Version XM_024994642.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGGCGTGGGG GGCCACCCCT TGTCCCCACA GGAGGAGGAG
    AAGTCGATCC TCCGGCACCG GCGCTGGCTG CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT
    ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236295
    Clone ID Related Accession (Same CDS sequence) XM_024994642.1 , XM_024994643.1
    Accession Version XM_024994643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGGCGTGGGG GGCCACCCCT TGTCCCCACA GGAGGAGGAG
    AAGTCGATCC TCCGGCACCG GCGCTGGCTG CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT
    ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850411.1
    CDS140..4285
    Translation

    Target ORF information:

    RefSeq Version XM_024994643.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGGCGTGGGG GGCCACCCCT TGTCCCCACA GGAGGAGGAG
    AAGTCGATCC TCCGGCACCG GCGCTGGCTG CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT
    ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236296
    Clone ID Related Accession (Same CDS sequence) XM_024994644.1
    Accession Version XM_024994644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTGCGTGTGG
    CGCTACGTCC GGGCCAGTGC CTCCTACTGC CCCTACCTGC CCCCGCTCTG CGACCCCAGG
    GACGGGCACC TGCTGGTGGA TGGGTGCTTC GTCAACAACG TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGGCGTGGGG GGCCACCCCT TGTCCCCACA GGAGGAGGAG
    AAGTCGATCC TCCGGCACCG GCGCTGGCTG CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT
    ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850412.1
    CDS145..4290
    Translation

    Target ORF information:

    RefSeq Version XM_024994644.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTGCGTGTGG
    CGCTACGTCC GGGCCAGTGC CTCCTACTGC CCCTACCTGC CCCCGCTCTG CGACCCCAGG
    GACGGGCACC TGCTGGTGGA TGGGTGCTTC GTCAACAACG TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGGCGTGGGG GGCCACCCCT TGTCCCCACA GGAGGAGGAG
    AAGTCGATCC TCCGGCACCG GCGCTGGCTG CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT
    ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236297
    Clone ID Related Accession (Same CDS sequence) XM_024994645.1
    Accession Version XM_024994645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGGCTC GGGACTAGGT
    GTACCCCCTC ACTCGGAGCT CACCAACCCC GCCAGCAACC TGTCAACAGT GGCAGTGCTG
    CCTGTGTGTG CCGAGGTGCC CATGGTGGCC TTCATGCTGG AGCTGCAGCA CGCTCTGCAA
    GCCATTGGCC CCACGCTCCT CCTCAACAGT GACATCATCC GGGCCCGCCT GGGGGCCTCT
    GCACTGGACA GCATCCAAGA ATTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGACACG
    CACCGCATCG TGCTCTACCA AACAGACACA TCGCTGACGC CCTGGACTGT GCGATGCCTG
    CGTCAGGCCG ACTGCATCCT CATCGTGGGG CTGGGCGACC AGGAGCCCAC ACTTGGCCAG
    CTGGAGCAGA TGCTGGAGAA CACGGCAGTG CGCGCCCTCA AGCAGCTGGT TCTGCTGCAC
    CGCGAGGAGG GCGCAGGCCC CACACGCACT GTGGAGTGGC TCAACATGCG CAGCTGGTGC
    TCGGGACACC TGCATCTGCG CTGTCCGCGC CGCCTCTTCT CACGCCGCAG CCCGGCCAAG
    CTGCACGAGC TCTACGAGAA GGTTTTCTCG AGGCGCGCAG ACCGGCACAG CGATTTCTCC
    CGCCTGGCGC GGGTGCTCAC GGGCAACACC ATCGCCCTGG TGCTGGGCGG GGGCGGAGCC
    AGGGGCTGCT CACACATCGG GGTGCTGAAG GCATTAGAGG AGGCGGGGGT CCCCGTTGAC
    CTGGTGGGTG GCACGTCCAT CGGCTCCTTC ATCGGGGCCC TGTACGCCGA GGAGCGGAGC
    GCCAGCCGCA CTAAACAGCG GGCCCGGGAG TGGGCTAAGA GCATGACTTC GGTGCTGGAG
    CCCGTACTGG ACCTCACCTA CCCCGTCACC TCCATGTTCA CGGGGTCAGC CTTCAACCGC
    AGCATCCACC GTGTCTTCCA GGACAAGCAG ATCGAGGACC TGTGGCTGCC ATACTTCAAC
    GTGACCACGG ATATCACCTC CTCAGCCATG CGTGTCCACA AAGATGGCTC CCTGTGGCGA
    TACGTGCGTG CCAGCATGAC TCTCTCGGGC TACCTGCCGC CGCTGTGCGA CCCCAAGGAT
    GGGCACCTCC TCATGGACGG CGGCTACATC AACAACCTGC CAGCGGACAT CGCCCGCAGC
    ATGGGTGCCA AGACAGTCAT CGCCATCGAC GTGGGGAGCC AGGACGAGAC AGACCTTAGC
    ACATACGGGG ACAGCCTGTC TGGCTGGTGG CTTCTGTGGA AGCGGCTGAA TCCCTGGGCA
    GACAAGATCA AGGTTCCAGA TATGGCAGAG ATCCAGTCTC GCCTGGCCTA CGTGTCCTGC
    GTGCGACAGC TGGAGGTTGT GAAGTCCAGC TCCTACTGTG AGTACCTGCG CCCACCCATC
    GACTGCTTCA AGACCATGGA CTTCGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG
    TATGGATCGA CCGTCTTTGG GGGCTGGAGC CGGGGCGACA TCATTGAAAA GATGCTCACA
    GACCGGTGGT CTGCCGACCT GAACGAGAGC CGCCGTGCAG ACGTGCTCGC CTTCCCCAGC
    TCTGGCTTCA CCGACTTGGC GGAGATAGTG TCCCGGATCG AGCCGCCCAC CACGAGCTAC
    GTTTCGGTTT CCGACGGTTG TGCTGATGGG GAGGAGTCGG ACTGTCTGAC GGAGTATGAG
    GAGGACGCGG GCCCTGAGTG CTCACGGGAC GAAGGGGGCT CTCCGGAGGG CGCAAGCCCC
    AGCACTGCCT TGGAGATGGG CGTGGGGGGC CACCCCTTGT CCCCACAGGA GGAGGAGAAG
    TCGATCCTCC GGCACCGGCG CTGGCTGCCG CAGGAACCCT CCAGCCCTGC TGCAGATACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850413.1
    CDS60..4202
    Translation

    Target ORF information:

    RefSeq Version XM_024994645.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGGCTC GGGACTAGGT
    GTACCCCCTC ACTCGGAGCT CACCAACCCC GCCAGCAACC TGTCAACAGT GGCAGTGCTG
    CCTGTGTGTG CCGAGGTGCC CATGGTGGCC TTCATGCTGG AGCTGCAGCA CGCTCTGCAA
    GCCATTGGCC CCACGCTCCT CCTCAACAGT GACATCATCC GGGCCCGCCT GGGGGCCTCT
    GCACTGGACA GCATCCAAGA ATTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGACACG
    CACCGCATCG TGCTCTACCA AACAGACACA TCGCTGACGC CCTGGACTGT GCGATGCCTG
    CGTCAGGCCG ACTGCATCCT CATCGTGGGG CTGGGCGACC AGGAGCCCAC ACTTGGCCAG
    CTGGAGCAGA TGCTGGAGAA CACGGCAGTG CGCGCCCTCA AGCAGCTGGT TCTGCTGCAC
    CGCGAGGAGG GCGCAGGCCC CACACGCACT GTGGAGTGGC TCAACATGCG CAGCTGGTGC
    TCGGGACACC TGCATCTGCG CTGTCCGCGC CGCCTCTTCT CACGCCGCAG CCCGGCCAAG
    CTGCACGAGC TCTACGAGAA GGTTTTCTCG AGGCGCGCAG ACCGGCACAG CGATTTCTCC
    CGCCTGGCGC GGGTGCTCAC GGGCAACACC ATCGCCCTGG TGCTGGGCGG GGGCGGAGCC
    AGGGGCTGCT CACACATCGG GGTGCTGAAG GCATTAGAGG AGGCGGGGGT CCCCGTTGAC
    CTGGTGGGTG GCACGTCCAT CGGCTCCTTC ATCGGGGCCC TGTACGCCGA GGAGCGGAGC
    GCCAGCCGCA CTAAACAGCG GGCCCGGGAG TGGGCTAAGA GCATGACTTC GGTGCTGGAG
    CCCGTACTGG ACCTCACCTA CCCCGTCACC TCCATGTTCA CGGGGTCAGC CTTCAACCGC
    AGCATCCACC GTGTCTTCCA GGACAAGCAG ATCGAGGACC TGTGGCTGCC ATACTTCAAC
    GTGACCACGG ATATCACCTC CTCAGCCATG CGTGTCCACA AAGATGGCTC CCTGTGGCGA
    TACGTGCGTG CCAGCATGAC TCTCTCGGGC TACCTGCCGC CGCTGTGCGA CCCCAAGGAT
    GGGCACCTCC TCATGGACGG CGGCTACATC AACAACCTGC CAGCGGACAT CGCCCGCAGC
    ATGGGTGCCA AGACAGTCAT CGCCATCGAC GTGGGGAGCC AGGACGAGAC AGACCTTAGC
    ACATACGGGG ACAGCCTGTC TGGCTGGTGG CTTCTGTGGA AGCGGCTGAA TCCCTGGGCA
    GACAAGATCA AGGTTCCAGA TATGGCAGAG ATCCAGTCTC GCCTGGCCTA CGTGTCCTGC
    GTGCGACAGC TGGAGGTTGT GAAGTCCAGC TCCTACTGTG AGTACCTGCG CCCACCCATC
    GACTGCTTCA AGACCATGGA CTTCGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG
    TATGGATCGA CCGTCTTTGG GGGCTGGAGC CGGGGCGACA TCATTGAAAA GATGCTCACA
    GACCGGTGGT CTGCCGACCT GAACGAGAGC CGCCGTGCAG ACGTGCTCGC CTTCCCCAGC
    TCTGGCTTCA CCGACTTGGC GGAGATAGTG TCCCGGATCG AGCCGCCCAC CACGAGCTAC
    GTTTCGGTTT CCGACGGTTG TGCTGATGGG GAGGAGTCGG ACTGTCTGAC GGAGTATGAG
    GAGGACGCGG GCCCTGAGTG CTCACGGGAC GAAGGGGGCT CTCCGGAGGG CGCAAGCCCC
    AGCACTGCCT TGGAGATGGG CGTGGGGGGC CACCCCTTGT CCCCACAGGA GGAGGAGAAG
    TCGATCCTCC GGCACCGGCG CTGGCTGCCG CAGGAACCCT CCAGCCCTGC TGCAGATACC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236298
    Clone ID Related Accession (Same CDS sequence) XM_024994646.1 , XM_024994647.1
    Accession Version XM_024994646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGAGGAGGAG AAGTCGATCC TCCGGCACCG GCGCTGGCTG
    CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850414.1
    CDS139..4254
    Translation

    Target ORF information:

    RefSeq Version XM_024994646.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGAGGAGGAG AAGTCGATCC TCCGGCACCG GCGCTGGCTG
    CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236298
    Clone ID Related Accession (Same CDS sequence) XM_024994646.1 , XM_024994647.1
    Accession Version XM_024994647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGAGGAGGAG AAGTCGATCC TCCGGCACCG GCGCTGGCTG
    CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850415.1
    CDS139..4254
    Translation

    Target ORF information:

    RefSeq Version XM_024994647.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGCAGG CTCGGGACTA
    GGTGTACCCC CTCACTCGGA GCTCACCAAC CCCGCCAGCA ACCTGTCAAC AGTGGCAGTG
    CTGCCTGTGT GTGCCGAGGT GCCCATGGTG GCCTTCATGC TGGAGCTGCA GCACGCTCTG
    CAAGCCATTG GCCCCACGCT CCTCCTCAAC AGTGACATCA TCCGGGCCCG CCTGGGGGCC
    TCTGCACTGG ACAGCATCCA AGAATTCCGG CTGTCAGGGT GGCTGGCCCA GCAGGAGGAC
    ACGCACCGCA TCGTGCTCTA CCAAACAGAC ACATCGCTGA CGCCCTGGAC TGTGCGATGC
    CTGCGTCAGG CCGACTGCAT CCTCATCGTG GGGCTGGGCG ACCAGGAGCC CACACTTGGC
    CAGCTGGAGC AGATGCTGGA GAACACGGCA GTGCGCGCCC TCAAGCAGCT GGTTCTGCTG
    CACCGCGAGG AGGGCGCAGG CCCCACACGC ACTGTGGAGT GGCTCAACAT GCGCAGCTGG
    TGCTCGGGAC ACCTGCATCT GCGCTGTCCG CGCCGCCTCT TCTCACGCCG CAGCCCGGCC
    AAGCTGCACG AGCTCTACGA GAAGGTTTTC TCGAGGCGCG CAGACCGGCA CAGCGATTTC
    TCCCGCCTGG CGCGGGTGCT CACGGGCAAC ACCATCGCCC TGGTGCTGGG CGGGGGCGGA
    GCCAGGGGCT GCTCACACAT CGGGGTGCTG AAGGCATTAG AGGAGGCGGG GGTCCCCGTT
    GACCTGGTGG GTGGCACGTC CATCGGCTCC TTCATCGGGG CCCTGTACGC CGAGGAGCGG
    AGCGCCAGCC GCACTAAACA GCGGGCCCGG GAGTGGGCTA AGAGCATGAC TTCGGTGCTG
    GAGCCCGTAC TGGACCTCAC CTACCCCGTC ACCTCCATGT TCACGGGGTC AGCCTTCAAC
    CGCAGCATCC ACCGTGTCTT CCAGGACAAG CAGATCGAGG ACCTGTGGCT GCCATACTTC
    AACGTGACCA CGGATATCAC CTCCTCAGCC ATGCGTGTCC ACAAAGATGG CTCCCTGTGG
    CGATACGTGC GTGCCAGCAT GACTCTCTCG GGCTACCTGC CGCCGCTGTG CGACCCCAAG
    GATGGGCACC TCCTCATGGA CGGCGGCTAC ATCAACAACC TGCCAGCGGA CATCGCCCGC
    AGCATGGGTG CCAAGACAGT CATCGCCATC GACGTGGGGA GCCAGGACGA GACAGACCTT
    AGCACATACG GGGACAGCCT GTCTGGCTGG TGGCTTCTGT GGAAGCGGCT GAATCCCTGG
    GCAGACAAGA TCAAGGTTCC AGATATGGCA GAGATCCAGT CTCGCCTGGC CTACGTGTCC
    TGCGTGCGAC AGCTGGAGGT TGTGAAGTCC AGCTCCTACT GTGAGTACCT GCGCCCACCC
    ATCGACTGCT TCAAGACCAT GGACTTCGGG AAGTTCGACC AGATCTATGA TGTGGGCTAC
    CAGTATGGAT CGACCGTCTT TGGGGGCTGG AGCCGGGGCG ACATCATTGA AAAGATGCTC
    ACAGACCGGT GGTCTGCCGA CCTGAACGAG AGCCGCCGTG CAGACGTGCT CGCCTTCCCC
    AGCTCTGGCT TCACCGACTT GGCGGAGATA GTGTCCCGGA TCGAGCCGCC CACCACGAGC
    TACGTTTCGG TTTCCGACGG TTGTGCTGAT GGGGAGGAGT CGGACTGTCT GACGGAGTAT
    GAGGAGGACG CGGGCCCTGA GTGCTCACGG GACGAAGGGG GCTCTCCGGA GGGCGCAAGC
    CCCAGCACTG CCTTGGAGAT GGAGGAGGAG AAGTCGATCC TCCGGCACCG GCGCTGGCTG
    CCGCAGGAAC CCTCCAGCCC TGCTGCAGAT ACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236299
    Clone ID Related Accession (Same CDS sequence) XM_024994648.1
    Accession Version XM_024994648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGGCTC GGGACTAGGT
    GTACCCCCTC ACTCGGAGCT CACCAACCCC GCCAGCAACC TGTCAACAGT GGCAGTGCTG
    CCTGTGTGTG CCGAGGTGCC CATGGTGGCC TTCATGCTGG AGCTGCAGCA CGCTCTGCAA
    GCCATTGGCC CCACGCTCCT CCTCAACAGT GACATCATCC GGGCCCGCCT GGGGGCCTCT
    GCACTGGACA GCATCCAAGA ATTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGACACG
    CACCGCATCG TGCTCTACCA AACAGACACA TCGCTGACGC CCTGGACTGT GCGATGCCTG
    CGTCAGGCCG ACTGCATCCT CATCGTGGGG CTGGGCGACC AGGAGCCCAC ACTTGGCCAG
    CTGGAGCAGA TGCTGGAGAA CACGGCAGTG CGCGCCCTCA AGCAGCTGGT TCTGCTGCAC
    CGCGAGGAGG GCGCAGGCCC CACACGCACT GTGGAGTGGC TCAACATGCG CAGCTGGTGC
    TCGGGACACC TGCATCTGCG CTGTCCGCGC CGCCTCTTCT CACGCCGCAG CCCGGCCAAG
    CTGCACGAGC TCTACGAGAA GGTTTTCTCG AGGCGCGCAG ACCGGCACAG CGATTTCTCC
    CGCCTGGCGC GGGTGCTCAC GGGCAACACC ATCGCCCTGG TGCTGGGCGG GGGCGGAGCC
    AGGGGCTGCT CACACATCGG GGTGCTGAAG GCATTAGAGG AGGCGGGGGT CCCCGTTGAC
    CTGGTGGGTG GCACGTCCAT CGGCTCCTTC ATCGGGGCCC TGTACGCCGA GGAGCGGAGC
    GCCAGCCGCA CTAAACAGCG GGCCCGGGAG TGGGCTAAGA GCATGACTTC GGTGCTGGAG
    CCCGTACTGG ACCTCACCTA CCCCGTCACC TCCATGTTCA CGGGGTCAGC CTTCAACCGC
    AGCATCCACC GTGTCTTCCA GGACAAGCAG ATCGAGGACC TGTGGCTGCC ATACTTCAAC
    GTGACCACGG ATATCACCTC CTCAGCCATG CGTGTCCACA AAGATGGCTC CCTGTGGCGA
    TACGTGCGTG CCAGCATGAC TCTCTCGGGC TACCTGCCGC CGCTGTGCGA CCCCAAGGAT
    GGGCACCTCC TCATGGACGG CGGCTACATC AACAACCTGC CAGCGGACAT CGCCCGCAGC
    ATGGGTGCCA AGACAGTCAT CGCCATCGAC GTGGGGAGCC AGGACGAGAC AGACCTTAGC
    ACATACGGGG ACAGCCTGTC TGGCTGGTGG CTTCTGTGGA AGCGGCTGAA TCCCTGGGCA
    GACAAGATCA AGGTTCCAGA TATGGCAGAG ATCCAGTCTC GCCTGGCCTA CGTGTCCTGC
    GTGCGACAGC TGGAGGTTGT GAAGTCCAGC TCCTACTGTG AGTACCTGCG CCCACCCATC
    GACTGCTTCA AGACCATGGA CTTCGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG
    TATGGATCGA CCGTCTTTGG GGGCTGGAGC CGGGGCGACA TCATTGAAAA GATGCTCACA
    GACCGGTGGT CTGCCGACCT GAACGAGAGC CGCCGTGCAG ACGTGCTCGC CTTCCCCAGC
    TCTGGCTTCA CCGACTTGGC GGAGATAGTG TCCCGGATCG AGCCGCCCAC CACGAGCTAC
    GTTTCGGTTT CCGACGGTTG TGCTGATGGG GAGGAGTCGG ACTGTCTGAC GGAGTATGAG
    GAGGACGCGG GCCCTGAGTG CTCACGGGAC GAAGGGGGCT CTCCGGAGGG CGCAAGCCCC
    AGCACTGCCT TGGAGATGGA GGAGGAGAAG TCGATCCTCC GGCACCGGCG CTGGCTGCCG
    CAGGAACCCT CCAGCCCTGC TGCAGATACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850416.1
    CDS107..4219
    Translation

    Target ORF information:

    RefSeq Version XM_024994648.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGGACGT CGAGGGCCGA GCCGACTGTG ATCTCCGGGG CGGACTTGGC TCCGTTGGAT 
    GGAGTCCCAA GCGTCCCGAC CCCCGGGGAA GGCTTGGCGG GGCCGGTATG CGATGCGCAG
    CCTGTGCCGT TCGTCCCGCA GGTGCTGGGC ATGATGATCG GGGCTGGAGT CGCGGTTCTG
    GTCACGGCCG TGCTCATCCT GCTGCTGGTG CGGAGGCTTC GAGTGCCGAA AGCTCCAGCC
    CCGGACGGCC CCCGTTACCG ATTCCGGAAG AGAGACAAAG TGCTTTTCTA TGGCCGGAAG
    ATCATGCGAA AGGTGTCACA ATCCACTTCT TCCTTAGTGG ACGCCTCCGT CTCCACCACT
    TCCCGCCCAC GCATGAAGAA GAAACTTAAG ATGCTCAACA TTGCCAAGAA GATCCTGCGC
    ATCCAGAAAG AGGCGCCCAC GCTGCAGCGG AAGGAGCCCC CGCCTGCTGT GCTGGAGGCT
    GACCTGACCG AGGGCGACCT GGCCAACTCC CACCTGCCCT CCGAGGTGCT CTACATGCTC
    AAGAATGTCC GGGTGCTGGG CCACTTTGAG AAGCCACTCT TCCTGGAGCT CTGCCGCCAC
    ATGGTCTTCC AGCGGCTCAG TCAGGGTGAC TATGTCTTCC GACCTGGCCA GCCGGATGCA
    AGCATCTATG TGGTACAGGA TGGACTCCTG GAGCTCTGTC TGCCGGGGCC TGACGGGAAG
    GAGTGTGTGG TGAAGGAAGT GGTTCCTGGG GACAGTGTCA ACAGCCTCCT GAGCATACTG
    GATGTCATCA CTGGTCACCA GCACCCACAG CGCACTGTGT CCGCCCGGGC AGCCCGAGAC
    TCCACGGTGC TGCGGCTGCC GGTGGAGGCC TTCTCAGCGG TATTCACCAA GTACCCCGAG
    AGCCTGGTGC GGGTGGTGCA GATCATCATG GTGAGGTTGC AGCGGGTCAC TTTCCTGGCA
    CTCCACAACT ACCTGGGACT GACCAATGAG CTCTTCAGCC ATGAGATCCA GCCACTGCGC
    CTCTTCCCTA GCCCGGGCCT CCCACCCCGC ACCAGCCCAG TGCGTGGCTC CAAGCGGATG
    GTCAGCACTG TGGCCTCAGA GGAGCTGAGG GAGACCCCTG GCCGGCCGTC TGACCCCACT
    GGGGCCCCAC TGCCCGGGAC TGGAGGTACC CAAGGGGACC CAGTGAAGCC CACATCCCTG
    GAAACCTCCT CAGCCCCCTT GCTGAGTCGC TGCATCTCCA TGCCAGTGGA TATCTCAGGC
    TTGCAGGGTG GGCCCCGCTC AGACTTCGAC ATGGCATATG AACGGGGCCG AATCTCTGTG
    TCCCTGCAAG AAGAGGCCTC AGGAGGGTTC CAGGCAGCCT CAGCTCGGAC TCCCAGTCAG
    GAGCCCCGGG AGCAGCCAGC AGGCGCCTGT GAGTACAGCT ATTGTGAGGA CGAGTCGGCC
    TCCGGCAGCT GCCCCTTCGG GCCCTACCAG GGCCGCCAGA CAAGCAGCAT CTTTGAGGCA
    GCAAAGCAGG AGCTGGCCAA GCTGATGCAG ATTGAGGACC CCACTCTTCT GAATAGTCGA
    GTTCTGCTAC ATCACGCCAA AGCTGGCACC ATCATCGCCC GCCAGGGGGA CCAGGATGTG
    AGCCTGCACT TTGTGCTGTG GGGCAGTCTG CACGTGTACC AGCGCATGAT CGACAAGGCC
    GAGGACGTGT GCCTGTTCGT GGTACAGCCC GGGGAGCTAG TGGGACAGTT GGCTGTGCTC
    ACCGGCGAGC CCCTCATCTT CACGCTTCGA GCCCAGCGTG ACTGTACCTT CCTGCGGATC
    TCCAAGTCCA ACTTCTACGA GATCATGCGT GCACAGCCCA GCGTGGTGCT GAGCGCCGCA
    CAAACCGTGG CCGCCAGGAT GTCGCCCTTC GTGCGCCAGA TGGACTTCGC CATCGACTGG
    ACAGCGGTGG AGGCGGGACG CGCACTGTAC AGGCAGGGAG ACCGTTCAGA CTGCACCTAC
    ATCGTGCTCA ACGGGCGGCT GCGTAGTGTC ATTCAGAGGG GCAGCGGCAA AAAAGAGCTG
    GTGGGCGAGT ACGGCCGCGG GGATCTCATA GGAGTGGTGG AGGCGCTGAC AAGGCAGCCA
    CGTGCCACGA CGGTGCACGC GGTGCGCGAC ACAGAGCTGG CCAAACTCCC CGAGGGCACC
    CTGGGCCACA TCAAACGTCG ATACCCGCAG GTCGTGACCC GCCTCATCCA CCTGCTGAGT
    CAGAAGATTC TGGGGAATTT GCAGCAGCTG CGAGGACCCT TCCCAGGCTC GGGACTAGGT
    GTACCCCCTC ACTCGGAGCT CACCAACCCC GCCAGCAACC TGTCAACAGT GGCAGTGCTG
    CCTGTGTGTG CCGAGGTGCC CATGGTGGCC TTCATGCTGG AGCTGCAGCA CGCTCTGCAA
    GCCATTGGCC CCACGCTCCT CCTCAACAGT GACATCATCC GGGCCCGCCT GGGGGCCTCT
    GCACTGGACA GCATCCAAGA ATTCCGGCTG TCAGGGTGGC TGGCCCAGCA GGAGGACACG
    CACCGCATCG TGCTCTACCA AACAGACACA TCGCTGACGC CCTGGACTGT GCGATGCCTG
    CGTCAGGCCG ACTGCATCCT CATCGTGGGG CTGGGCGACC AGGAGCCCAC ACTTGGCCAG
    CTGGAGCAGA TGCTGGAGAA CACGGCAGTG CGCGCCCTCA AGCAGCTGGT TCTGCTGCAC
    CGCGAGGAGG GCGCAGGCCC CACACGCACT GTGGAGTGGC TCAACATGCG CAGCTGGTGC
    TCGGGACACC TGCATCTGCG CTGTCCGCGC CGCCTCTTCT CACGCCGCAG CCCGGCCAAG
    CTGCACGAGC TCTACGAGAA GGTTTTCTCG AGGCGCGCAG ACCGGCACAG CGATTTCTCC
    CGCCTGGCGC GGGTGCTCAC GGGCAACACC ATCGCCCTGG TGCTGGGCGG GGGCGGAGCC
    AGGGGCTGCT CACACATCGG GGTGCTGAAG GCATTAGAGG AGGCGGGGGT CCCCGTTGAC
    CTGGTGGGTG GCACGTCCAT CGGCTCCTTC ATCGGGGCCC TGTACGCCGA GGAGCGGAGC
    GCCAGCCGCA CTAAACAGCG GGCCCGGGAG TGGGCTAAGA GCATGACTTC GGTGCTGGAG
    CCCGTACTGG ACCTCACCTA CCCCGTCACC TCCATGTTCA CGGGGTCAGC CTTCAACCGC
    AGCATCCACC GTGTCTTCCA GGACAAGCAG ATCGAGGACC TGTGGCTGCC ATACTTCAAC
    GTGACCACGG ATATCACCTC CTCAGCCATG CGTGTCCACA AAGATGGCTC CCTGTGGCGA
    TACGTGCGTG CCAGCATGAC TCTCTCGGGC TACCTGCCGC CGCTGTGCGA CCCCAAGGAT
    GGGCACCTCC TCATGGACGG CGGCTACATC AACAACCTGC CAGCGGACAT CGCCCGCAGC
    ATGGGTGCCA AGACAGTCAT CGCCATCGAC GTGGGGAGCC AGGACGAGAC AGACCTTAGC
    ACATACGGGG ACAGCCTGTC TGGCTGGTGG CTTCTGTGGA AGCGGCTGAA TCCCTGGGCA
    GACAAGATCA AGGTTCCAGA TATGGCAGAG ATCCAGTCTC GCCTGGCCTA CGTGTCCTGC
    GTGCGACAGC TGGAGGTTGT GAAGTCCAGC TCCTACTGTG AGTACCTGCG CCCACCCATC
    GACTGCTTCA AGACCATGGA CTTCGGGAAG TTCGACCAGA TCTATGATGT GGGCTACCAG
    TATGGATCGA CCGTCTTTGG GGGCTGGAGC CGGGGCGACA TCATTGAAAA GATGCTCACA
    GACCGGTGGT CTGCCGACCT GAACGAGAGC CGCCGTGCAG ACGTGCTCGC CTTCCCCAGC
    TCTGGCTTCA CCGACTTGGC GGAGATAGTG TCCCGGATCG AGCCGCCCAC CACGAGCTAC
    GTTTCGGTTT CCGACGGTTG TGCTGATGGG GAGGAGTCGG ACTGTCTGAC GGAGTATGAG
    GAGGACGCGG GCCCTGAGTG CTCACGGGAC GAAGGGGGCT CTCCGGAGGG CGCAAGCCCC
    AGCACTGCCT TGGAGATGGA GGAGGAGAAG TCGATCCTCC GGCACCGGCG CTGGCTGCCG
    CAGGAACCCT CCAGCCCTGC TGCAGATACC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236300
    Clone ID Related Accession (Same CDS sequence) XM_024994649.1 , XM_024994651.1 , XM_024994650.1 , XM_024994654.1
    Accession Version XM_024994649.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850417.1
    CDS999..5030
    Translation

    Target ORF information:

    RefSeq Version XM_024994649.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236300
    Clone ID Related Accession (Same CDS sequence) XM_024994649.1 , XM_024994651.1 , XM_024994650.1 , XM_024994654.1
    Accession Version XM_024994651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850419.1
    CDS126..4157
    Translation

    Target ORF information:

    RefSeq Version XM_024994651.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236300
    Clone ID Related Accession (Same CDS sequence) XM_024994649.1 , XM_024994651.1 , XM_024994650.1 , XM_024994654.1
    Accession Version XM_024994650.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850418.1
    CDS992..5023
    Translation

    Target ORF information:

    RefSeq Version XM_024994650.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236300
    Clone ID Related Accession (Same CDS sequence) XM_024994649.1 , XM_024994651.1 , XM_024994650.1 , XM_024994654.1
    Accession Version XM_024994654.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850422.1
    CDS193..4224
    Translation

    Target ORF information:

    RefSeq Version XM_024994654.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGGC GTGGGGGGCC ACCCCTTGTC CCCACAGGAG
    GAGGAGAAGT CGATCCTCCG GCACCGGCGC TGGCTGCCGC AGGAACCCTC CAGCCCTGCT
    GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236301
    Clone ID Related Accession (Same CDS sequence) XM_024994652.1
    Accession Version XM_024994652.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGGCGTG GGGGGCCACC CCTTGTCCCC ACAGGAGGAG
    GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG CTGCCGCAGG AACCCTCCAG CCCTGCTGCA
    GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850420.1
    CDS279..4307
    Translation

    Target ORF information:

    RefSeq Version XM_024994652.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994652.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGGCGTG GGGGGCCACC CCTTGTCCCC ACAGGAGGAG
    GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG CTGCCGCAGG AACCCTCCAG CCCTGCTGCA
    GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236302
    Clone ID Related Accession (Same CDS sequence) XM_024994655.1
    Accession Version XM_024994655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGAG GAGGAGAAGT CGATCCTCCG GCACCGGCGC
    TGGCTGCCGC AGGAACCCTC CAGCCCTGCT GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850423.1
    CDS1116..5117
    Translation

    Target ORF information:

    RefSeq Version XM_024994655.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGCAGGCTCG
    GGACTAGGTG TACCCCCTCA CTCGGAGCTC ACCAACCCCG CCAGCAACCT GTCAACAGTG
    GCAGTGCTGC CTGTGTGTGC CGAGGTGCCC ATGGTGGCCT TCATGCTGGA GCTGCAGCAC
    GCTCTGCAAG CCATTGGCCC CACGCTCCTC CTCAACAGTG ACATCATCCG GGCCCGCCTG
    GGGGCCTCTG CACTGGACAG CATCCAAGAA TTCCGGCTGT CAGGGTGGCT GGCCCAGCAG
    GAGGACACGC ACCGCATCGT GCTCTACCAA ACAGACACAT CGCTGACGCC CTGGACTGTG
    CGATGCCTGC GTCAGGCCGA CTGCATCCTC ATCGTGGGGC TGGGCGACCA GGAGCCCACA
    CTTGGCCAGC TGGAGCAGAT GCTGGAGAAC ACGGCAGTGC GCGCCCTCAA GCAGCTGGTT
    CTGCTGCACC GCGAGGAGGG CGCAGGCCCC ACACGCACTG TGGAGTGGCT CAACATGCGC
    AGCTGGTGCT CGGGACACCT GCATCTGCGC TGTCCGCGCC GCCTCTTCTC ACGCCGCAGC
    CCGGCCAAGC TGCACGAGCT CTACGAGAAG GTTTTCTCGA GGCGCGCAGA CCGGCACAGC
    GATTTCTCCC GCCTGGCGCG GGTGCTCACG GGCAACACCA TCGCCCTGGT GCTGGGCGGG
    GGCGGAGCCA GGGGCTGCTC ACACATCGGG GTGCTGAAGG CATTAGAGGA GGCGGGGGTC
    CCCGTTGACC TGGTGGGTGG CACGTCCATC GGCTCCTTCA TCGGGGCCCT GTACGCCGAG
    GAGCGGAGCG CCAGCCGCAC TAAACAGCGG GCCCGGGAGT GGGCTAAGAG CATGACTTCG
    GTGCTGGAGC CCGTACTGGA CCTCACCTAC CCCGTCACCT CCATGTTCAC GGGGTCAGCC
    TTCAACCGCA GCATCCACCG TGTCTTCCAG GACAAGCAGA TCGAGGACCT GTGGCTGCCA
    TACTTCAACG TGACCACGGA TATCACCTCC TCAGCCATGC GTGTCCACAA AGATGGCTCC
    CTGTGGCGAT ACGTGCGTGC CAGCATGACT CTCTCGGGCT ACCTGCCGCC GCTGTGCGAC
    CCCAAGGATG GGCACCTCCT CATGGACGGC GGCTACATCA ACAACCTGCC AGCGGACATC
    GCCCGCAGCA TGGGTGCCAA GACAGTCATC GCCATCGACG TGGGGAGCCA GGACGAGACA
    GACCTTAGCA CATACGGGGA CAGCCTGTCT GGCTGGTGGC TTCTGTGGAA GCGGCTGAAT
    CCCTGGGCAG ACAAGATCAA GGTTCCAGAT ATGGCAGAGA TCCAGTCTCG CCTGGCCTAC
    GTGTCCTGCG TGCGACAGCT GGAGGTTGTG AAGTCCAGCT CCTACTGTGA GTACCTGCGC
    CCACCCATCG ACTGCTTCAA GACCATGGAC TTCGGGAAGT TCGACCAGAT CTATGATGTG
    GGCTACCAGT ATGGATCGAC CGTCTTTGGG GGCTGGAGCC GGGGCGACAT CATTGAAAAG
    ATGCTCACAG ACCGGTGGTC TGCCGACCTG AACGAGAGCC GCCGTGCAGA CGTGCTCGCC
    TTCCCCAGCT CTGGCTTCAC CGACTTGGCG GAGATAGTGT CCCGGATCGA GCCGCCCACC
    ACGAGCTACG TTTCGGTTTC CGACGGTTGT GCTGATGGGG AGGAGTCGGA CTGTCTGACG
    GAGTATGAGG AGGACGCGGG CCCTGAGTGC TCACGGGACG AAGGGGGCTC TCCGGAGGGC
    GCAAGCCCCA GCACTGCCTT GGAGATGGAG GAGGAGAAGT CGATCCTCCG GCACCGGCGC
    TGGCTGCCGC AGGAACCCTC CAGCCCTGCT GCAGATACCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38278
    Clone ID Related Accession (Same CDS sequence) NM_001076547.2 , NM_001076547.3 , XM_024994653.1
    Accession Version NM_001076547.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product neuropathy target esterase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC112748.1, DAAA02019500.1, EE958553.1, EE907606.1, DY124832.1, EE378680.1 and AW632372.1. On Oct 7, 2009 this sequence version replaced NM_001076547.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822088, SAMN02822093 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGAGGAG GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG
    CTGCCGCAGG AACCCTCCAG CCCTGCTGCA GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070015.2
    CDS109..4107
    Translation

    Target ORF information:

    RefSeq Version NM_001076547.2
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076547.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGAGGAG GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG
    CTGCCGCAGG AACCCTCCAG CCCTGCTGCA GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38278
    Clone ID Related Accession (Same CDS sequence) NM_001076547.2 , NM_001076547.3 , XM_024994653.1
    Accession Version NM_001076547.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product neuropathy target esterase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000007.1. On Dec 20, 2018 this sequence version replaced NM_001076547.2. ##Evidence-Data-START## CDS exon combination :: GGUY01102951.1, GGUZ01083371.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGAGGAG GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG
    CTGCCGCAGG AACCCTCCAG CCCTGCTGCA GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001070015.2
    CDS109..4107
    Translation

    Target ORF information:

    RefSeq Version NM_001076547.3
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001076547.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGAGGAG GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG
    CTGCCGCAGG AACCCTCCAG CCCTGCTGCA GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38278
    Clone ID Related Accession (Same CDS sequence) NM_001076547.2 , NM_001076547.3 , XM_024994653.1
    Accession Version XM_024994653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product neuropathy target esterase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGAGGAG GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG
    CTGCCGCAGG AACCCTCCAG CCCTGCTGCA GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850421.1
    CDS168..4166
    Translation

    Target ORF information:

    RefSeq Version XM_024994653.1
    Organism Bos taurus(cattle)
    Definition Bos taurus patatin like phospholipase domain containing 6 (PNPLA6), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCCC CGCTGCAAAC CGGAATGGTG CTGGGCATGA TGATCGGGGC TGGAGTCGCG 
    GTTCTGGTCA CGGCCGTGCT CATCCTGCTG CTGGTGCGGA GGCTTCGAGT GCCGAAAGCT
    CCAGCCCCGG ACGGCCCCCG TTACCGATTC CGGAAGAGAG ACAAAGTGCT TTTCTATGGC
    CGGAAGATCA TGCGAAAGGT GTCACAATCC ACTTCTTCCT TAGTGGACGC CTCCGTCTCC
    ACCACTTCCC GCCCACGCAT GAAGAAGAAA CTTAAGATGC TCAACATTGC CAAGAAGATC
    CTGCGCATCC AGAAAGAGGC GCCCACGCTG CAGCGGAAGG AGCCCCCGCC TGCTGTGCTG
    GAGGCTGACC TGACCGAGGG CGACCTGGCC AACTCCCACC TGCCCTCCGA GGTGCTCTAC
    ATGCTCAAGA ATGTCCGGGT GCTGGGCCAC TTTGAGAAGC CACTCTTCCT GGAGCTCTGC
    CGCCACATGG TCTTCCAGCG GCTCAGTCAG GGTGACTATG TCTTCCGACC TGGCCAGCCG
    GATGCAAGCA TCTATGTGGT ACAGGATGGA CTCCTGGAGC TCTGTCTGCC GGGGCCTGAC
    GGGAAGGAGT GTGTGGTGAA GGAAGTGGTT CCTGGGGACA GTGTCAACAG CCTCCTGAGC
    ATACTGGATG TCATCACTGG TCACCAGCAC CCACAGCGCA CTGTGTCCGC CCGGGCAGCC
    CGAGACTCCA CGGTGCTGCG GCTGCCGGTG GAGGCCTTCT CAGCGGTATT CACCAAGTAC
    CCCGAGAGCC TGGTGCGGGT GGTGCAGATC ATCATGGTGA GGTTGCAGCG GGTCACTTTC
    CTGGCACTCC ACAACTACCT GGGACTGACC AATGAGCTCT TCAGCCATGA GATCCAGCCA
    CTGCGCCTCT TCCCTAGCCC GGGCCTCCCA CCCCGCACCA GCCCAGTGCG TGGCTCCAAG
    CGGATGGTCA GCACTGTGGC CTCAGAGGAG CTGAGGGAGA CCCCTGGCCG GCCGTCTGAC
    CCCACTGGGG CCCCACTGCC CGGGACTGGA GGTACCCAAG GGGACCCAGT GAAGCCCACA
    TCCCTGGAAA CCTCCTCAGC CCCCTTGCTG AGTCGCTGCA TCTCCATGCC AGTGGATATC
    TCAGGCTTGC AGGGTGGGCC CCGCTCAGAC TTCGACATGG CATATGAACG GGGCCGAATC
    TCTGTGTCCC TGCAAGAAGA GGCCTCAGGA GGGTTCCAGG CAGCCTCAGC TCGGACTCCC
    AGTCAGGAGC CCCGGGAGCA GCCAGCAGGC GCCTGTGAGT ACAGCTATTG TGAGGACGAG
    TCGGCCTCCG GCAGCTGCCC CTTCGGGCCC TACCAGGGCC GCCAGACAAG CAGCATCTTT
    GAGGCAGCAA AGCAGGAGCT GGCCAAGCTG ATGCAGATTG AGGACCCCAC TCTTCTGAAT
    AGTCGAGTTC TGCTACATCA CGCCAAAGCT GGCACCATCA TCGCCCGCCA GGGGGACCAG
    GATGTGAGCC TGCACTTTGT GCTGTGGGGC AGTCTGCACG TGTACCAGCG CATGATCGAC
    AAGGCCGAGG ACGTGTGCCT GTTCGTGGTA CAGCCCGGGG AGCTAGTGGG ACAGTTGGCT
    GTGCTCACCG GCGAGCCCCT CATCTTCACG CTTCGAGCCC AGCGTGACTG TACCTTCCTG
    CGGATCTCCA AGTCCAACTT CTACGAGATC ATGCGTGCAC AGCCCAGCGT GGTGCTGAGC
    GCCGCACAAA CCGTGGCCGC CAGGATGTCG CCCTTCGTGC GCCAGATGGA CTTCGCCATC
    GACTGGACAG CGGTGGAGGC GGGACGCGCA CTGTACAGGC AGGGAGACCG TTCAGACTGC
    ACCTACATCG TGCTCAACGG GCGGCTGCGT AGTGTCATTC AGAGGGGCAG CGGCAAAAAA
    GAGCTGGTGG GCGAGTACGG CCGCGGGGAT CTCATAGGAG TGGTGGAGGC GCTGACAAGG
    CAGCCACGTG CCACGACGGT GCACGCGGTG CGCGACACAG AGCTGGCCAA ACTCCCCGAG
    GGCACCCTGG GCCACATCAA ACGTCGATAC CCGCAGGTCG TGACCCGCCT CATCCACCTG
    CTGAGTCAGA AGATTCTGGG GAATTTGCAG CAGCTGCGAG GACCCTTCCC AGGCTCGGGA
    CTAGGTGTAC CCCCTCACTC GGAGCTCACC AACCCCGCCA GCAACCTGTC AACAGTGGCA
    GTGCTGCCTG TGTGTGCCGA GGTGCCCATG GTGGCCTTCA TGCTGGAGCT GCAGCACGCT
    CTGCAAGCCA TTGGCCCCAC GCTCCTCCTC AACAGTGACA TCATCCGGGC CCGCCTGGGG
    GCCTCTGCAC TGGACAGCAT CCAAGAATTC CGGCTGTCAG GGTGGCTGGC CCAGCAGGAG
    GACACGCACC GCATCGTGCT CTACCAAACA GACACATCGC TGACGCCCTG GACTGTGCGA
    TGCCTGCGTC AGGCCGACTG CATCCTCATC GTGGGGCTGG GCGACCAGGA GCCCACACTT
    GGCCAGCTGG AGCAGATGCT GGAGAACACG GCAGTGCGCG CCCTCAAGCA GCTGGTTCTG
    CTGCACCGCG AGGAGGGCGC AGGCCCCACA CGCACTGTGG AGTGGCTCAA CATGCGCAGC
    TGGTGCTCGG GACACCTGCA TCTGCGCTGT CCGCGCCGCC TCTTCTCACG CCGCAGCCCG
    GCCAAGCTGC ACGAGCTCTA CGAGAAGGTT TTCTCGAGGC GCGCAGACCG GCACAGCGAT
    TTCTCCCGCC TGGCGCGGGT GCTCACGGGC AACACCATCG CCCTGGTGCT GGGCGGGGGC
    GGAGCCAGGG GCTGCTCACA CATCGGGGTG CTGAAGGCAT TAGAGGAGGC GGGGGTCCCC
    GTTGACCTGG TGGGTGGCAC GTCCATCGGC TCCTTCATCG GGGCCCTGTA CGCCGAGGAG
    CGGAGCGCCA GCCGCACTAA ACAGCGGGCC CGGGAGTGGG CTAAGAGCAT GACTTCGGTG
    CTGGAGCCCG TACTGGACCT CACCTACCCC GTCACCTCCA TGTTCACGGG GTCAGCCTTC
    AACCGCAGCA TCCACCGTGT CTTCCAGGAC AAGCAGATCG AGGACCTGTG GCTGCCATAC
    TTCAACGTGA CCACGGATAT CACCTCCTCA GCCATGCGTG TCCACAAAGA TGGCTCCCTG
    TGGCGATACG TGCGTGCCAG CATGACTCTC TCGGGCTACC TGCCGCCGCT GTGCGACCCC
    AAGGATGGGC ACCTCCTCAT GGACGGCGGC TACATCAACA ACCTGCCAGC GGACATCGCC
    CGCAGCATGG GTGCCAAGAC AGTCATCGCC ATCGACGTGG GGAGCCAGGA CGAGACAGAC
    CTTAGCACAT ACGGGGACAG CCTGTCTGGC TGGTGGCTTC TGTGGAAGCG GCTGAATCCC
    TGGGCAGACA AGATCAAGGT TCCAGATATG GCAGAGATCC AGTCTCGCCT GGCCTACGTG
    TCCTGCGTGC GACAGCTGGA GGTTGTGAAG TCCAGCTCCT ACTGTGAGTA CCTGCGCCCA
    CCCATCGACT GCTTCAAGAC CATGGACTTC GGGAAGTTCG ACCAGATCTA TGATGTGGGC
    TACCAGTATG GATCGACCGT CTTTGGGGGC TGGAGCCGGG GCGACATCAT TGAAAAGATG
    CTCACAGACC GGTGGTCTGC CGACCTGAAC GAGAGCCGCC GTGCAGACGT GCTCGCCTTC
    CCCAGCTCTG GCTTCACCGA CTTGGCGGAG ATAGTGTCCC GGATCGAGCC GCCCACCACG
    AGCTACGTTT CGGTTTCCGA CGGTTGTGCT GATGGGGAGG AGTCGGACTG TCTGACGGAG
    TATGAGGAGG ACGCGGGCCC TGAGTGCTCA CGGGACGAAG GGGGCTCTCC GGAGGGCGCA
    AGCCCCAGCA CTGCCTTGGA GATGGAGGAG GAGAAGTCGA TCCTCCGGCA CCGGCGCTGG
    CTGCCGCAGG AACCCTCCAG CCCTGCTGCA GATACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.