FAM135B cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	FAM135B
Entrez Gene ID	618755
Full Name	family with sequence similarity 135 member B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005215241.3	XP_005215298.1	protein FAM135B isoform X1
XM_005215241.4	XP_005215298.2	protein FAM135B isoform X1
XM_025001765.1	XP_024857533.1	protein FAM135B isoform X1
NM_001078091.1	NP_001071559.1	protein FAM135B
NM_001078091.2	NP_001071559.2	protein FAM135B
XM_010811865.2	XP_010810167.1	protein FAM135B isoform X2
XM_025001766.1	XP_024857534.1	protein FAM135B isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FAM135B	NP_001071559.1
Pan troglodytes (chimpanzee)	FAM135B	XP_001140728.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FAM135B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAM135B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36597	NM_001078091.1 Latest version!	Bos taurus family with sequence similarity 135 member B (FAM135B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa222443	NM_001078091.2 Latest version!	Bos taurus family with sequence similarity 135 member B (FAM135B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222443	XM_005215241.4 Latest version!	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222443	XM_025001765.1 Latest version!	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222444	XM_025001766.1 Latest version!	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa56778	XM_010811865.2 Latest version!	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa25800	XM_005215241.3 Latest version!	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FAM135B ( NM_001078091.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001071559.1 Bos taurus family with sequence similarity 135 member B (FAM135B), mRNA.

Price & Availability↑

CloneID	OBa36597
Clone ID Related Accession (Same CDS sequence)	NM_001078091.1
Accession Version	NM_001078091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	protein FAM135B
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from BC123873.1. On Nov 21, 2006 this sequence version replaced XM_871081.1. ##Evidence-Data-START## Transcript exon combination :: BC123873.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145431, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGGTAATCA GGGAGCCAAC TCCTTGGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071559.1
CDS	131..1033
Translation	MSEVQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIVGQTDSSSPHSACVHDSTLRSRVFQILYRNEEVPINDAVIFRAHLLLDGERVEEAVSEVDFQLKVDLHFTDSEQQLRDMAGVPVISSRMLGLHFHPRRGLHHQVPVMFDYFHLSVISVTIHAALVALQQPLISFTRPGRGSWLGKGGPDTGPEQSSLSLENLVFGAGYCKPTSSEGSFYVPSENCMQHAYKWHRELCFLLLHAYRGLRAYFLTILRDIPELPRMELESLAVEETLSQLCSELQVIREPTPWF

Target ORF information:

RefSeq Version	NM_001078091.1
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 135 member B (FAM135B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078091.1

ORF Insert Sequence:

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGGTAATCA GGGAGCCAAC TCCTTGGTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135B ( NM_001078091.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001071559.2 Bos taurus family with sequence similarity 135 member B (FAM135B), mRNA.

Price & Availability↑

CloneID	OBa222443
Clone ID Related Accession (Same CDS sequence)	XM_005215241.4 , XM_025001765.1 , NM_001078091.2
Accession Version	NM_001078091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-09
Organism	Bos taurus(cattle)
Product	protein FAM135B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000014.1. On or before Aug 10, 2019 this sequence version replaced XM_005215241.4, NM_001078091.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145431, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071559.2
CDS	173..4387
Translation	MSEVQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIVGQTDSSSPHSACVHDSTLRSRVFQILYRNEEVPINDAVIFRAHLLLDGERVEEAVSEVDFQLKVDLHFTDSEQQLRDMAGVPVISSRMLGLHFHPRRGLHHQVPVMFDYFHLSVISVTIHAALVALQQPLISFTRPGRGSWLGKGGPDTGPEQSSLSLENLVFGAGYCKPTSSEGSFYVPSENCMQHAYKWHRELCFLLLHAYRGLRAYFLTILRDIPELPRMELESLAVEETLSQLCSELQMLNNPEKIAEQISKDLAWLASHLMALWTQFLDTVTLHSQVTTYLTQEHHTLRVRRFSEAFFYMEHQKLAVLTFQENLIQTHSQLSLDIRNSEYLTTMPPLPAECLDIDGDWNTLPVIFEDRYVDCPATGCNLNIYPNFDVPATSPAIMNLNNREENLVVNRKSPLRENLVLASMKSSPTDSDEEVIRCPGPSKNVATPSHTDVCSESQVYLTVGEFQSKAGVPEVDHQTGPRSDVGGHLLADEDLPRRSPGPEDGQAPALTYIDVKSSNKNPFRTEPTVPTGAQHESGCSRAKHELGRTERSKGVAGGSHHCAVSSEKTTLHELSSLGKGVGQGSKMVLLSLKVSPSEPCEPLNSSLRDPLDIKGLAKDPHIEEKEEVCVLSGVIKRSASIISDSGIESEPSSVAWSEARSRTLELPSDRDFLHQLVRRHALHRNSLEGGHTESNTSLPSGIQASLTSISSLPFEEEERELALTKLTKSVSAPQISSPEESAEDADSTRHGGGPSETSAVPTESTNPSGRCLQPSDGPTIREAGVDHGLVEEVSDADNLQGPGYIDIPKGQEHQFDPQGPCCLDGRTDHPPGVETKGLGLKVPHLLGLESPGHRVLPTELVETPKGVPGDSDVGTGAPLDSDMLDVESFTYPRVPEVSCAPAAKTIDVDSAGEQSSSLGITEDTAPSRGADTFPEDEREAGAGSTVTLSVPSQVTGNQEPRAGTTILLSHMTSAESSSLDSLKAVEVVNLSVSCAATCLPFSSVPKETPARAGFSSKQTPFPITHQPLGSFGVIPNHSRKLEEEVSERMFSFYQAKEKFKKELKIDGFLYSDLSVLASDIPYFPPEEEEENLEDGIHLVVCVHGLDGNSADLRLVKTFIELGLPGGKLDFLMSEKNQMDTFADFDTMTDRLLDEIIQHIQLYNLSISRISFIGHSLGNIIIRSVLTRPRFRYYLNKLHTFLSLSGPHLGTLYNNSALVSTGLWLMQKLKKSGSLLQLTFRDNADLRKCFLYQLSQKTGLQYFKNVVLVASPQDRYVPFHSARIEMCKTALRDRHTGPVYAEMINNLLRPLVDAKDCTLIRHNVFHALPNTANTLIGRAAHIAVLDSELFLEKFFLVAGLNYFK

Target ORF information:

RefSeq Version	NM_001078091.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 135 member B (FAM135B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001078091.2

ORF Insert Sequence:

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135B ( XM_005215241.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215298.2 Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa222443
Clone ID Related Accession (Same CDS sequence)	XM_005215241.4 , XM_025001765.1 , NM_001078091.2
Accession Version	XM_005215241.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein FAM135B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215241.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215298.2
CDS	173..4387
Translation	MSEVQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIVGQTDSSSPHSACVHDSTLRSRVFQILYRNEEVPINDAVIFRAHLLLDGERVEEAVSEVDFQLKVDLHFTDSEQQLRDMAGVPVISSRMLGLHFHPRRGLHHQVPVMFDYFHLSVISVTIHAALVALQQPLISFTRPGRGSWLGKGGPDTGPEQSSLSLENLVFGAGYCKPTSSEGSFYVPSENCMQHAYKWHRELCFLLLHAYRGLRAYFLTILRDIPELPRMELESLAVEETLSQLCSELQMLNNPEKIAEQISKDLAWLASHLMALWTQFLDTVTLHSQVTTYLTQEHHTLRVRRFSEAFFYMEHQKLAVLTFQENLIQTHSQLSLDIRNSEYLTTMPPLPAECLDIDGDWNTLPVIFEDRYVDCPATGCNLNIYPNFDVPATSPAIMNLNNREENLVVNRKSPLRENLVLASMKSSPTDSDEEVIRCPGPSKNVATPSHTDVCSESQVYLTVGEFQSKAGVPEVDHQTGPRSDVGGHLLADEDLPRRSPGPEDGQAPALTYIDVKSSNKNPFRTEPTVPTGAQHESGCSRAKHELGRTERSKGVAGGSHHCAVSSEKTTLHELSSLGKGVGQGSKMVLLSLKVSPSEPCEPLNSSLRDPLDIKGLAKDPHIEEKEEVCVLSGVIKRSASIISDSGIESEPSSVAWSEARSRTLELPSDRDFLHQLVRRHALHRNSLEGGHTESNTSLPSGIQASLTSISSLPFEEEERELALTKLTKSVSAPQISSPEESAEDADSTRHGGGPSETSAVPTESTNPSGRCLQPSDGPTIREAGVDHGLVEEVSDADNLQGPGYIDIPKGQEHQFDPQGPCCLDGRTDHPPGVETKGLGLKVPHLLGLESPGHRVLPTELVETPKGVPGDSDVGTGAPLDSDMLDVESFTYPRVPEVSCAPAAKTIDVDSAGEQSSSLGITEDTAPSRGADTFPEDEREAGAGSTVTLSVPSQVTGNQEPRAGTTILLSHMTSAESSSLDSLKAVEVVNLSVSCAATCLPFSSVPKETPARAGFSSKQTPFPITHQPLGSFGVIPNHSRKLEEEVSERMFSFYQAKEKFKKELKIDGFLYSDLSVLASDIPYFPPEEEEENLEDGIHLVVCVHGLDGNSADLRLVKTFIELGLPGGKLDFLMSEKNQMDTFADFDTMTDRLLDEIIQHIQLYNLSISRISFIGHSLGNIIIRSVLTRPRFRYYLNKLHTFLSLSGPHLGTLYNNSALVSTGLWLMQKLKKSGSLLQLTFRDNADLRKCFLYQLSQKTGLQYFKNVVLVASPQDRYVPFHSARIEMCKTALRDRHTGPVYAEMINNLLRPLVDAKDCTLIRHNVFHALPNTANTLIGRAAHIAVLDSELFLEKFFLVAGLNYFK

Target ORF information:

RefSeq Version	XM_005215241.4
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215241.4

ORF Insert Sequence:

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135B ( XM_025001765.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857533.1 Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222443
Clone ID Related Accession (Same CDS sequence)	XM_005215241.4 , XM_025001765.1 , NM_001078091.2
Accession Version	XM_025001765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein FAM135B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857533.1
CDS	171..4385
Translation	MSEVQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIVGQTDSSSPHSACVHDSTLRSRVFQILYRNEEVPINDAVIFRAHLLLDGERVEEAVSEVDFQLKVDLHFTDSEQQLRDMAGVPVISSRMLGLHFHPRRGLHHQVPVMFDYFHLSVISVTIHAALVALQQPLISFTRPGRGSWLGKGGPDTGPEQSSLSLENLVFGAGYCKPTSSEGSFYVPSENCMQHAYKWHRELCFLLLHAYRGLRAYFLTILRDIPELPRMELESLAVEETLSQLCSELQMLNNPEKIAEQISKDLAWLASHLMALWTQFLDTVTLHSQVTTYLTQEHHTLRVRRFSEAFFYMEHQKLAVLTFQENLIQTHSQLSLDIRNSEYLTTMPPLPAECLDIDGDWNTLPVIFEDRYVDCPATGCNLNIYPNFDVPATSPAIMNLNNREENLVVNRKSPLRENLVLASMKSSPTDSDEEVIRCPGPSKNVATPSHTDVCSESQVYLTVGEFQSKAGVPEVDHQTGPRSDVGGHLLADEDLPRRSPGPEDGQAPALTYIDVKSSNKNPFRTEPTVPTGAQHESGCSRAKHELGRTERSKGVAGGSHHCAVSSEKTTLHELSSLGKGVGQGSKMVLLSLKVSPSEPCEPLNSSLRDPLDIKGLAKDPHIEEKEEVCVLSGVIKRSASIISDSGIESEPSSVAWSEARSRTLELPSDRDFLHQLVRRHALHRNSLEGGHTESNTSLPSGIQASLTSISSLPFEEEERELALTKLTKSVSAPQISSPEESAEDADSTRHGGGPSETSAVPTESTNPSGRCLQPSDGPTIREAGVDHGLVEEVSDADNLQGPGYIDIPKGQEHQFDPQGPCCLDGRTDHPPGVETKGLGLKVPHLLGLESPGHRVLPTELVETPKGVPGDSDVGTGAPLDSDMLDVESFTYPRVPEVSCAPAAKTIDVDSAGEQSSSLGITEDTAPSRGADTFPEDEREAGAGSTVTLSVPSQVTGNQEPRAGTTILLSHMTSAESSSLDSLKAVEVVNLSVSCAATCLPFSSVPKETPARAGFSSKQTPFPITHQPLGSFGVIPNHSRKLEEEVSERMFSFYQAKEKFKKELKIDGFLYSDLSVLASDIPYFPPEEEEENLEDGIHLVVCVHGLDGNSADLRLVKTFIELGLPGGKLDFLMSEKNQMDTFADFDTMTDRLLDEIIQHIQLYNLSISRISFIGHSLGNIIIRSVLTRPRFRYYLNKLHTFLSLSGPHLGTLYNNSALVSTGLWLMQKLKKSGSLLQLTFRDNADLRKCFLYQLSQKTGLQYFKNVVLVASPQDRYVPFHSARIEMCKTALRDRHTGPVYAEMINNLLRPLVDAKDCTLIRHNVFHALPNTANTLIGRAAHIAVLDSELFLEKFFLVAGLNYFK

Target ORF information:

RefSeq Version	XM_025001765.1
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001765.1

ORF Insert Sequence:

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135B ( XM_025001766.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857534.1 Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X3, mRNA.

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CloneID	OBa222444
Clone ID Related Accession (Same CDS sequence)	XM_025001766.1
Accession Version	XM_025001766.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	protein FAM135B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857534.1
CDS	171..3806
Translation	MSEVQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIVGQTDSSSPHSACVHDSTLRSRVFQILYRNEEVPINDAVIFRAHLLLDGERVEEAVSEVDFQLKVDLHFTDSEQQLRDMAGVPVISSRMLGLHFHPRRGLHHQVPVMFDYFHLSVISVTIHAALVALQQPLISFTRPGRGSWLGKGGPDTGPEQSSLSLENLVFGAGYCKPTSSEGSFYVPSENCMQHAYKWHRELCFLLLHAYRGLRAYFLTILRDIPELPRMELESLAVEETLSQLCSELQMLNNPEKIAEQISKDLAWLASHLMALWTQFLDTVTLHSQVTTYLTQEHHTLRVRRFSEAFFYMEHQKLAVLTFQENLIQTHSQLSLDIRNSEYLTTMPPLPAECLDIDGDWNTLPVIFEDRYVDCPATGCNLNIYPNFDVPATSPAIMNLNNREENLVVNRKSPLRENLVLASMKSSPTDSDEEVIRCPGPSKNVATPSHTDVCSESQVYLTVGEFQSKAGVPEVDHQTGPRSDVGGHLLADEDLPRRSPGPEDGQAPALTYIDVKSSNKNPFRTEPTVPTGAQHESGCSRAKHELGRTERSKGVAGGSHHCAVSSEKTTLHELSSLGKGVGQGSKMVLLSLKVSPSEPCEPLNSSLRDPLDIKGLAKDPHIEEKEEVCVLSGVIKRSASIISDSGIESEPSSVAWSEARSRTLELPSDRDFLHQLVRRHALHRNSLEGGHTESNTSLPSGIQASLTSISSLPFEEEERELALTKLTKSVSAPQISSPEESAEDADSTRHGGGPSETSAVPTESTNPSGRCLQPSDGPTIREAGVDHGLVEEVSDADNLQGPGYIDIPKGQEHQFDPQGPCCLDGRTDHPPGVETKGLGLKVPHLLGLESPGHRVLPTELVETPKGVPGDSDVGTGAPLDSDMLDVESFTYPRVPEVSCAPAAKTIDVDSAGEQSSSLGITEDTAPSRGADTFPEDEREAGAGSTVTLSVPSQVTGNQEPRAGTTILLSHMTSAESSSLDSLKAVEVVNLSVSCAATCLPFSSVPKETPARAGFSSKQTPFPITHQPLGSFGVIPNHSRKLEEEVSERMFSFYQAKEKFKKELKIDGFLYSDLSVLASDIPYFPPEEEEENLEDGIHLVVCVHGLDGNSADLRLVKTFIELGLPGGKLDFLMSEKNQMDTFADFDTMTDRLLDEIIQHIQLYNLSISRIR

Target ORF information:

RefSeq Version	XM_025001766.1
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001766.1

ORF Insert Sequence:

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTAT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135B ( XM_010811865.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810167.1 Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X2, mRNA.

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CloneID	OBa56778
Clone ID Related Accession (Same CDS sequence)	XM_010811865.2
Accession Version	XM_010811865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM135B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTGT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810167.1
CDS	210..3845
Translation	MSEVQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIVGQTDSSSPHSACVHDSTLRSRVFQILYRNEEVPINDAVIFRAHLLLDGERVEEAVSEVDFQLKVDLHFTDSEQQLRDMAGVPVISSRVLGLHFHPRRGLHHQVPVMFDYFHLSVISVTIHAALVALQQPLISFTRPGRGSWLGKGGPDTGPEQSSLSLENLVFGAGYCKPTSSEGSFYVPSENCMQHAYKWHRELCFLLLHAYRGLRAYFLTILRDIPELPRMELESLAVEETLSQLCSELQMLNNPEKIAEQISKDLAWLASHLMALWTQFLDTVTLHSQVTTYLTQEHHTLRVRRFSEAFFYMEHQKLAVLTFQENLIQTHSQLSLDIRNSEYLTTMPPLPAECLDIDGDWNTLPVIFEDRYVDCPATGCNLNIYPNFDVPATSPAIMNLNNREENLVVNRKSPLRENLVLASMKSSPTDSDEEVIRCPGPSKNVATPSHTDVCSESQVYLTVGEFQSKAGVPEVDHQTGPRSDVGGHLLADEDLPRRSPGPEDGQAPALTYIDVKSSNKNPFRTEPTVPTGAQHESGCSRAKHELGRTERSKGVAGGSHHCAVSSEKTTLHELSSLGKGVGQGSKMVLLSLKVSPSEPCEPLNSSLRDPLDIKGLAKDPHIEEKEEVCVLSGVIKRSASIISDSGIESEPSSVAWSEARSRTLELPSDRDFLHQLVRRHALHRNSLEGGHTESNTSLPSGIQASLTSISSLPFEEEERELALTKLTKSVSAPQISSPEESAEDADSTRHGGGPSETSAVPTESTNPSGRCLQPSDGPTIREAGVDHGLVEEVSDADNLQGPGYIDIPKGQEHQFDPQGPCCLDGRTDHPPGVETKGLGLKVPHLLGLESPGHRVLPTELVETPKGVPGDSDVGTGAPLDSDMLDVESFTYPRVPEVSCAPAAKTIDVDSAGEQSSSLGITEDTAPSRGADTFPEDEREAGAGSTVTLSVPSQVTGNQEPRAGTTILLSHMTSAESSSLDSLKAVEVVNLSVSCAATCLPFSSVPKETPARAGFSSKQTPFPITHQPLGSFGVIPNHSRKLEEEVSERMFSFYQAKEKFKKELKIDGFLYSDLSVLASDIPYFPPEEEEENLEDGIHLVVCVHGLDGNSADLRLVKTFIELGLPGGKLDFLMSEKNQMDTFADFDTMTDRLLDEIIQHIQLYNLSISRIR

Target ORF information:

RefSeq Version	XM_010811865.2
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811865.2

ORF Insert Sequence:

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTGT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM135B ( XM_005215241.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215298.1 Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X1, mRNA.

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CloneID	OBa25800
Clone ID Related Accession (Same CDS sequence)	XM_005215241.3
Accession Version	XM_005215241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein FAM135B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTGT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215298.1
CDS	205..4419
Translation	MSEVQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIVGQTDSSSPHSACVHDSTLRSRVFQILYRNEEVPINDAVIFRAHLLLDGERVEEAVSEVDFQLKVDLHFTDSEQQLRDMAGVPVISSRVLGLHFHPRRGLHHQVPVMFDYFHLSVISVTIHAALVALQQPLISFTRPGRGSWLGKGGPDTGPEQSSLSLENLVFGAGYCKPTSSEGSFYVPSENCMQHAYKWHRELCFLLLHAYRGLRAYFLTILRDIPELPRMELESLAVEETLSQLCSELQMLNNPEKIAEQISKDLAWLASHLMALWTQFLDTVTLHSQVTTYLTQEHHTLRVRRFSEAFFYMEHQKLAVLTFQENLIQTHSQLSLDIRNSEYLTTMPPLPAECLDIDGDWNTLPVIFEDRYVDCPATGCNLNIYPNFDVPATSPAIMNLNNREENLVVNRKSPLRENLVLASMKSSPTDSDEEVIRCPGPSKNVATPSHTDVCSESQVYLTVGEFQSKAGVPEVDHQTGPRSDVGGHLLADEDLPRRSPGPEDGQAPALTYIDVKSSNKNPFRTEPTVPTGAQHESGCSRAKHELGRTERSKGVAGGSHHCAVSSEKTTLHELSSLGKGVGQGSKMVLLSLKVSPSEPCEPLNSSLRDPLDIKGLAKDPHIEEKEEVCVLSGVIKRSASIISDSGIESEPSSVAWSEARSRTLELPSDRDFLHQLVRRHALHRNSLEGGHTESNTSLPSGIQASLTSISSLPFEEEERELALTKLTKSVSAPQISSPEESAEDADSTRHGGGPSETSAVPTESTNPSGRCLQPSDGPTIREAGVDHGLVEEVSDADNLQGPGYIDIPKGQEHQFDPQGPCCLDGRTDHPPGVETKGLGLKVPHLLGLESPGHRVLPTELVETPKGVPGDSDVGTGAPLDSDMLDVESFTYPRVPEVSCAPAAKTIDVDSAGEQSSSLGITEDTAPSRGADTFPEDEREAGAGSTVTLSVPSQVTGNQEPRAGTTILLSHMTSAESSSLDSLKAVEVVNLSVSCAATCLPFSSVPKETPARAGFSSKQTPFPITHQPLGSFGVIPNHSRKLEEEVSERMFSFYQAKEKFKKELKIDGFLYSDLSVLASDIPYFPPEEEEENLEDGIHLVVCVHGLDGNSADLRLVKTFIELGLPGGKLDFLMSEKNQMDTFADFDTMTDRLLDEIIQHIQLYNLSISRISFIGHSLGNIIIRSVLTRPRFRYYLNKLHTFLSLSGPHLGTLYNNSALVSTGLWLMQKLKKSGSLLQLTFRDNADLRKCFLYQLSQKTGLQYFKNVVLVASPQDRYVPFHSARIEMCKTALRDRHTGPVYAEMINNLLRPLVDAKDCTLIRHNVFHALPNTANTLIGRAAHIAVLDSELFLEKFFLVAGLNYFK

Target ORF information:

RefSeq Version	XM_005215241.3
Organism	Bos taurus(cattle)
Definition	Bos taurus family with sequence similarity 135 member B (FAM135B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215241.3

ORF Insert Sequence:

ATGTCTGAAG TACAAGGAAC AGTTGAGTTT TCTGTAGAGC TACATAAATT TTATAATGTG 
GATCTGTTTC AGAGAGGGTA TTATCAGATC CGAGTGACCT TGAAGGTGTC CTCAAGGATC 
CCCCACAGAC TGAGCGCCTC CATCGTTGGG CAGACAGACA GCAGCAGCCC ACACTCAGCC 
TGCGTCCACG ACAGCACCTT GCGCAGCCGG GTCTTCCAGA TCTTATACAG GAACGAGGAG 
GTGCCCATCA ATGATGCCGT GATCTTCCGA GCTCATCTGC TCTTAGACGG TGAAAGGGTG 
GAAGAAGCGG TAAGTGAAGT CGATTTCCAG CTCAAGGTGG ATTTGCACTT TACGGACAGT 
GAACAACAGC TGAGGGACAT GGCCGGGGTC CCCGTGATCA GCAGCCGTGT GCTGGGCCTG 
CATTTCCACC CCCGGAGAGG CCTGCATCAC CAGGTGCCCG TCATGTTCGA CTATTTCCAC 
CTGTCGGTGA TCTCGGTGAC CATCCACGCC GCCCTGGTGG CCCTGCAGCA GCCGCTGATC 
AGTTTTACTC GCCCAGGAAG AGGCTCCTGG CTTGGGAAAG GCGGCCCAGA CACAGGACCA 
GAGCAGTCTA GCCTTTCCCT GGAAAACCTG GTCTTCGGAG CTGGATACTG CAAGCCGACC 
TCCTCAGAGG GGAGCTTCTA CGTGCCATCT GAGAACTGCA TGCAGCACGC TTACAAGTGG 
CACCGGGAGC TGTGCTTCCT GCTCTTGCAC GCCTACCGGG GACTGCGTGC CTACTTCCTG 
ACCATCCTGA GGGACATCCC CGAGCTGCCG CGCATGGAGC TGGAGTCACT TGCTGTTGAA 
GAAACACTCT CTCAGCTGTG CTCAGAGCTT CAGATGTTGA ATAACCCAGA GAAGATAGCT 
GAGCAGATAA GTAAGGATCT CGCCTGGCTG GCCTCCCACC TGATGGCCCT GTGGACCCAG 
TTCCTGGACA CAGTGACGCT GCACTCCCAA GTGACCACTT ATCTCACCCA GGAGCATCAC 
ACCTTGCGGG TCCGAAGGTT TTCTGAGGCC TTCTTTTATA TGGAGCACCA AAAACTTGCA 
GTCCTGACTT TTCAGGAAAA CCTGATCCAG ACCCACAGCC AGCTGTCCCT GGATATCCGG 
AATTCGGAGT ACCTCACCAC CATGCCCCCG CTGCCCGCAG AGTGCTTGGA CATTGATGGA 
GACTGGAACA CCCTGCCCGT TATCTTCGAG GACAGATATG TGGACTGCCC AGCCACAGGA 
TGTAATTTGA ATATTTACCC TAATTTTGAT GTTCCAGCAA CAAGTCCAGC AATAATGAAC 
CTAAACAACA GAGAGGAGAA CCTTGTTGTA AATAGAAAAT CACCTCTGAG GGAAAACCTA 
GTCTTGGCCT CCATGAAATC ATCCCCCACA GACTCTGATG AGGAGGTTAT TAGGTGTCCA 
GGGCCCAGTA AGAATGTCGC CACACCCAGT CATACGGACG TGTGCTCTGA ATCTCAGGTG 
TATCTGACAG TTGGTGAATT CCAGAGCAAG GCAGGTGTGC CTGAAGTTGA CCATCAGACT 
GGCCCAAGGT CTGATGTTGG GGGGCATCTG CTGGCAGATG AGGATCTGCC CAGGAGGAGC 
CCTGGTCCAG AGGATGGCCA GGCCCCAGCG CTGACCTACA TTGATGTGAA ATCTAGCAAT 
AAGAACCCCT TCAGAACAGA ACCCACAGTG CCCACTGGTG CCCAGCACGA GAGTGGGTGC 
TCCAGAGCCA AGCACGAATT AGGCAGAACT GAGCGAAGCA AAGGGGTAGC CGGTGGAAGT 
CATCACTGTG CCGTCTCTTC AGAGAAAACC ACCCTGCATG AGTTAAGCAG TCTAGGAAAG 
GGGGTGGGCC AAGGGAGCAA GATGGTGCTG CTGAGTCTGA AGGTCAGCCC CTCGGAGCCC 
TGCGAGCCCC TGAACTCTTC TTTGAGGGAC CCTTTGGACA TCAAGGGTTT GGCAAAGGAC 
CCTCACATAG AGGAGAAGGA GGAAGTGTGT GTCCTCTCTG GGGTCATCAA GAGATCTGCT 
TCCATCATCT CCGACTCAGG CATTGAGAGC GAGCCAAGCT CTGTCGCCTG GTCCGAGGCT 
CGCAGCAGAA CCCTGGAGCT ACCCAGTGAC AGGGACTTCT TGCACCAGCT GGTCCGAAGA 
CATGCCCTGC ACAGAAACTC CCTGGAAGGG GGGCACACTG AGAGCAACAC GAGCTTGCCG 
AGCGGGATCC AGGCCTCCCT CACGTCCATT AGCTCTTTGC CTTTCGAGGA GGAGGAGCGG 
GAACTGGCAC TCACGAAGCT GACCAAGTCA GTGTCCGCCC CCCAGATCAG TAGCCCCGAG 
GAGTCTGCTG AAGATGCGGA CTCCACGCGG CATGGGGGAG GTCCCTCTGA AACTTCAGCT 
GTGCCCACCG AGAGCACCAA CCCCTCAGGA CGCTGCCTTC AACCCAGCGA TGGCCCCACA 
ATCCGGGAAG CTGGGGTCGA CCATGGCCTT GTGGAGGAAG TCTCAGATGC TGACAACCTG 
CAGGGCCCCG GTTACATAGA TATCCCCAAA GGTCAAGAGC ATCAGTTTGA CCCCCAAGGC 
CCCTGTTGTC TGGATGGCAG GACTGACCAC CCTCCAGGTG TTGAAACCAA AGGTCTGGGT 
TTAAAAGTAC CACACCTCCT CGGACTTGAA TCACCTGGGC ACAGAGTTTT GCCCACAGAA 
CTCGTGGAGA CCCCGAAAGG CGTGCCTGGA GACTCCGACG TGGGGACAGG AGCTCCTCTC 
GACAGCGACA TGTTGGATGT TGAGAGTTTC ACCTACCCCA GGGTGCCTGA AGTGAGCTGT 
GCACCAGCCG CCAAAACCAT CGACGTGGAT TCTGCAGGTG AGCAAAGCAG CTCCCTTGGC 
ATCACGGAGG ATACGGCACC GAGCAGAGGA GCTGACACCT TCCCAGAGGA TGAGCGTGAA 
GCAGGCGCAG GCTCCACTGT GACTCTGTCC GTTCCCTCCC AGGTTACGGG AAACCAGGAG 
CCCAGGGCAG GCACCACCAT CCTGCTGTCC CACATGACCT CTGCAGAGAG CTCCTCCCTG 
GACAGCCTGA AAGCTGTCGA GGTCGTCAAC CTGTCTGTGT CCTGCGCTGC CACCTGTCTC 
CCTTTCTCGT CTGTGCCCAA GGAGACCCCT GCCAGGGCTG GATTCTCTTC CAAACAGACC 
CCGTTCCCCA TCACTCATCA ACCTTTGGGC TCCTTTGGAG TCATTCCTAA CCATTCCAGA 
AAGCTGGAGG AGGAAGTCAG CGAAAGGATG TTCAGTTTTT ATCAGGCCAA AGAGAAATTT 
AAAAAAGAGC TGAAGATTGA TGGATTTCTG TACAGTGACT TATCTGTACT AGCTTCTGAC 
ATACCGTATT TCCCACCAGA GGAAGAGGAA GAAAATTTGG AAGATGGGAT TCACCTGGTA 
GTCTGTGTCC ATGGCCTGGA TGGGAACAGT GCAGACCTCC GGCTGGTAAA GACTTTCATA 
GAACTGGGGC TCCCTGGAGG AAAACTGGAT TTCCTCATGT CTGAGAAGAA TCAGATGGAC 
ACGTTTGCGG ATTTTGATAC CATGACCGAT CGGTTACTGG ATGAAATAAT TCAGCACATC 
CAGTTGTACA ACCTCTCCAT ATCGAGAATT AGCTTCATCG GCCATTCTCT CGGCAACATC 
ATCATCCGAT CGGTCCTCAC GCGGCCTCGG TTCCGGTATT ACCTCAACAA ACTCCACACC 
TTCCTGTCAC TGTCTGGGCC CCACCTGGGG ACCCTGTACA ACAACAGCGC GCTGGTCAGC 
ACAGGTTTGT GGCTCATGCA GAAACTGAAG AAATCCGGGT CGCTTTTGCA GCTGACCTTC 
AGGGATAATG CTGATCTGCG CAAATGTTTC CTCTACCAAC TAAGCCAAAA GACAGGTCTG 
CAGTATTTTA AAAACGTTGT GCTGGTTGCT TCTCCCCAAG ACCGCTACGT GCCCTTTCAT 
TCAGCCAGGA TCGAGATGTG TAAGACCGCC CTCAGAGACA GACACACAGG CCCTGTTTAT 
GCGGAAATGA TCAACAACCT TCTTCGCCCT CTGGTGGACG CCAAGGACTG CACTTTGATC 
CGACACAATG TATTCCACGC CTTGCCCAAC ACTGCCAACA CCCTGATTGG CCGAGCGGCT 
CACATCGCTG TGCTGGACTC CGAGCTCTTC CTGGAGAAGT TCTTCTTGGT GGCAGGACTC 
AACTACTTCA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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FAM135B cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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