SPHK1 cDNA ORF clone, Bos taurus(cattle)

The following SPHK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227032 XM_002696204.6
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981057.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981055.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981061.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981056.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981059.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981060.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981058.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227033 XM_024981054.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227034 XM_024981064.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227034 XM_024981063.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa227031 XM_024981051.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa227031 XM_024981053.1
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa30451 XM_002696204.5
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa30451 XM_005195854.3
Latest version!
Bos taurus sphingosine kinase 1 (SPHK1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227032
    Clone ID Related Accession (Same CDS sequence) XM_002696204.6
    Accession Version XM_002696204.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696204.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTTGGCT CCCCAGTCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGC GGGCGGCTCC CGGAACACGC TCCCAAGGCC CTGCCACGTG
    CTGGTGTTGT TAAATCCGCG CGGGGGCAAG GGCAAAGCCC TGCAGCTCTT CAGGAGCCAC
    GTACAGCCGC TGCTGGCCCA GGCCGACGTC TCCTTCACGC TGATGCTCAC TGAGCGGCGG
    AACCACGCCC GGGAGCTGGT GCGTGCGGAG GACCTGAGAC GCTGGGACGC GCTGGTGGTC
    ATGTCCGGAG ATGGGCTGAT CCACGAGGTG GTGAACGGGC TCATGGAGCG GCCTGACTGG
    GAGACCGCCA TCCAGAAGCC CCTGTGTAGC CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC
    GCTTCTTTGA ACTATTACGC CGGGTACGAG CAGGTGACTA AGGAAGACCT CCTGACCAAC
    TGCACGCAGC TGCTGTGCCG CCGGCTGCTG GCGCCCATGG ACCTGCTATC CCTGCAGACC
    GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC AGCCTGGCGT GGGGTTTCAT CGCCGACGTG
    GATCTGGAGA GCGAGAAGTT TCGGCGCCTG GGCGAGATGC GCTTCACTCT AGGCGCCTGC
    CTGCGCCTGG TGGCCCTGCG CACCTACCGG GGCAGCCTGG CCTACCTGCC CGCAGAAACG
    CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT CTGGCCCCGC AGGACCCCGC GGACACCCAT
    GTGGTGCCCC TGGAGCAGCC AGTGCCTCCT CACTGGACGG TGGTGCCGGA GCAGGACTTT
    GTGCTGGTGT TAGCACTCCT GCATTCCCAC CTGGCCAGTG ACCTTTTCAC CGCCCCCATG
    GGCCGCTGTT CGGCTGGCGC CATGCATCTG TTCTACGTGC GTGCTGGCGT GTCCCGGGCC
    ATGCTGCTGC GCCTCTTCCT GGCCATGGAG AAAGGCAGGC ACATGGAGTG CCACTGCCCC
    CACCTGGTCT ATGTGCCTGT GGTTGCCTTC CGCCTGGAGC CCAAGGATGG GAAGGGTGTG
    TTTACTGTGG ATGGGGAACT GCTGATCAGC GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC
    TACTTCTGGA TGGTCAGTGG CTACAGGGAG CCCCCACCCT CTCTGAAACC ACAGCCCTCA
    CCCCGCAGGA AGTCTCAGCA GACGCTGCCC CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696250.4
    CDS603..2030
    Translation

    Target ORF information:

    RefSeq Version XM_002696204.6
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696204.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTTGGCT CCCCAGTCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGC GGGCGGCTCC CGGAACACGC TCCCAAGGCC CTGCCACGTG
    CTGGTGTTGT TAAATCCGCG CGGGGGCAAG GGCAAAGCCC TGCAGCTCTT CAGGAGCCAC
    GTACAGCCGC TGCTGGCCCA GGCCGACGTC TCCTTCACGC TGATGCTCAC TGAGCGGCGG
    AACCACGCCC GGGAGCTGGT GCGTGCGGAG GACCTGAGAC GCTGGGACGC GCTGGTGGTC
    ATGTCCGGAG ATGGGCTGAT CCACGAGGTG GTGAACGGGC TCATGGAGCG GCCTGACTGG
    GAGACCGCCA TCCAGAAGCC CCTGTGTAGC CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC
    GCTTCTTTGA ACTATTACGC CGGGTACGAG CAGGTGACTA AGGAAGACCT CCTGACCAAC
    TGCACGCAGC TGCTGTGCCG CCGGCTGCTG GCGCCCATGG ACCTGCTATC CCTGCAGACC
    GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC AGCCTGGCGT GGGGTTTCAT CGCCGACGTG
    GATCTGGAGA GCGAGAAGTT TCGGCGCCTG GGCGAGATGC GCTTCACTCT AGGCGCCTGC
    CTGCGCCTGG TGGCCCTGCG CACCTACCGG GGCAGCCTGG CCTACCTGCC CGCAGAAACG
    CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT CTGGCCCCGC AGGACCCCGC GGACACCCAT
    GTGGTGCCCC TGGAGCAGCC AGTGCCTCCT CACTGGACGG TGGTGCCGGA GCAGGACTTT
    GTGCTGGTGT TAGCACTCCT GCATTCCCAC CTGGCCAGTG ACCTTTTCAC CGCCCCCATG
    GGCCGCTGTT CGGCTGGCGC CATGCATCTG TTCTACGTGC GTGCTGGCGT GTCCCGGGCC
    ATGCTGCTGC GCCTCTTCCT GGCCATGGAG AAAGGCAGGC ACATGGAGTG CCACTGCCCC
    CACCTGGTCT ATGTGCCTGT GGTTGCCTTC CGCCTGGAGC CCAAGGATGG GAAGGGTGTG
    TTTACTGTGG ATGGGGAACT GCTGATCAGC GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC
    TACTTCTGGA TGGTCAGTGG CTACAGGGAG CCCCCACCCT CTCTGAAACC ACAGCCCTCA
    CCCCGCAGGA AGTCTCAGCA GACGCTGCCC CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836824.1
    CDS323..1591
    Translation

    Target ORF information:

    RefSeq Version XM_024981056.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836829.1
    CDS766..2034
    Translation

    Target ORF information:

    RefSeq Version XM_024981061.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981054.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836822.1
    CDS489..1757
    Translation

    Target ORF information:

    RefSeq Version XM_024981054.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981054.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981058.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836826.1
    CDS767..2035
    Translation

    Target ORF information:

    RefSeq Version XM_024981058.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981058.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981055.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836823.1
    CDS456..1724
    Translation

    Target ORF information:

    RefSeq Version XM_024981055.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981055.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981057.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836825.1
    CDS850..2118
    Translation

    Target ORF information:

    RefSeq Version XM_024981057.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981057.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836827.1
    CDS581..1849
    Translation

    Target ORF information:

    RefSeq Version XM_024981059.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227033
    Clone ID Related Accession (Same CDS sequence) XM_024981056.1 , XM_024981061.1 , XM_024981054.1 , XM_024981058.1 , XM_024981055.1 , XM_024981057.1 , XM_024981059.1 , XM_024981060.1
    Accession Version XM_024981060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836828.1
    CDS376..1644
    Translation

    Target ORF information:

    RefSeq Version XM_024981060.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATGCAG GCGCCGCGTT TCCACCCGGG TGCCCGAGTT GTGATCCTCA GCCCCTAGTG 
    GCGGTTCCGG TTTGTGTTTA CAGCGGGCGG CTCCCGGAAC ACGCTCCCAA GGCCCTGCCA
    CGTGCTGGTG TTGTTAAATC CGCGCGGGGG CAAGGGCAAA GCCCTGCAGC TCTTCAGGAG
    CCACGTACAG CCGCTGCTGG CCCAGGCCGA CGTCTCCTTC ACGCTGATGC TCACTGCCGC
    GCCCTCTGCA CAGAGCGGCG GAACCACGCC CGGGAGCTGG TGCGTGCGGA GGACCTGAGA
    CGCTGGGACG CGCTGGTGGT CATGTCCGGA GATGGGCTGA TCCACGAGGT GGTGAACGGG
    CTCATGGAGC GGCCTGACTG GGAGACCGCC ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA
    GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG AACTATTACG CCGGGTACGA GCAGGTGACT
    AAGGAAGACC TCCTGACCAA CTGCACGCAG CTGCTGTGCC GCCGGCTGCT GGCGCCCATG
    GACCTGCTAT CCCTGCAGAC CGCCTGCGGG CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG
    TGGGGTTTCA TCGCCGACGT GGATCTGGAG AGCGAGAAGT TTCGGCGCCT GGGCGAGATG
    CGCTTCACTC TAGGCGCCTG CCTGCGCCTG GTGGCCCTGC GCACCTACCG GGGCAGCCTG
    GCCTACCTGC CCGCAGAAAC GCTGGCTTCC AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG
    CAGGACCCCG CGGACACCCA TGTGGTGCCC CTGGAGCAGC CAGTGCCTCC TCACTGGACG
    GTGGTGCCGG AGCAGGACTT TGTGCTGGTG TTAGCACTCC TGCATTCCCA CCTGGCCAGT
    GACCTTTTCA CCGCCCCCAT GGGCCGCTGT TCGGCTGGCG CCATGCATCT GTTCTACGTG
    CGTGCTGGCG TGTCCCGGGC CATGCTGCTG CGCCTCTTCC TGGCCATGGA GAAAGGCAGG
    CACATGGAGT GCCACTGCCC CCACCTGGTC TATGTGCCTG TGGTTGCCTT CCGCCTGGAG
    CCCAAGGATG GGAAGGGTGT GTTTACTGTG GATGGGGAAC TGCTGATCAG CGAGGCTGTC
    CAGGGCCAGG TTCTCCCCAA CTACTTCTGG ATGGTCAGTG GCTACAGGGA GCCCCCACCC
    TCTCTGAAAC CACAGCCCTC ACCCCGCAGG AAGTCTCAGC AGACGCTGCC CCCATCAGAG
    CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227034
    Clone ID Related Accession (Same CDS sequence) XM_024981063.1 , XM_024981064.1
    Accession Version XM_024981063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGCAG CGGGCGGCTC CCGGAACACG CTCCCAAGGC CCTGCCACGT GCTGGTGTTG 
    TTAAATCCGC GCGGGGGCAA GGGCAAAGCC CTGCAGCTCT TCAGGAGCCA CGTACAGCCG
    CTGCTGGCCC AGGCCGACGT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCC
    CGGGAGCTGG TGCGTGCGGA GGACCTGAGA CGCTGGGACG CGCTGGTGGT CATGTCCGGA
    GATGGGCTGA TCCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC
    ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG
    AACTATTACG CCGGGTACGA GCAGGTGACT AAGGAAGACC TCCTGACCAA CTGCACGCAG
    CTGCTGTGCC GCCGGCTGCT GGCGCCCATG GACCTGCTAT CCCTGCAGAC CGCCTGCGGG
    CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG TGGGGTTTCA TCGCCGACGT GGATCTGGAG
    AGCGAGAAGT TTCGGCGCCT GGGCGAGATG CGCTTCACTC TAGGCGCCTG CCTGCGCCTG
    GTGGCCCTGC GCACCTACCG GGGCAGCCTG GCCTACCTGC CCGCAGAAAC GCTGGCTTCC
    AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG CAGGACCCCG CGGACACCCA TGTGGTGCCC
    CTGGAGCAGC CAGTGCCTCC TCACTGGACG GTGGTGCCGG AGCAGGACTT TGTGCTGGTG
    TTAGCACTCC TGCATTCCCA CCTGGCCAGT GACCTTTTCA CCGCCCCCAT GGGCCGCTGT
    TCGGCTGGCG CCATGCATCT GTTCTACGTG CGTGCTGGCG TGTCCCGGGC CATGCTGCTG
    CGCCTCTTCC TGGCCATGGA GAAAGGCAGG CACATGGAGT GCCACTGCCC CCACCTGGTC
    TATGTGCCTG TGGTTGCCTT CCGCCTGGAG CCCAAGGATG GGAAGGGTGT GTTTACTGTG
    GATGGGGAAC TGCTGATCAG CGAGGCTGTC CAGGGCCAGG TTCTCCCCAA CTACTTCTGG
    ATGGTCAGTG GCTACAGGGA GCCCCCACCC TCTCTGAAAC CACAGCCCTC ACCCCGCAGG
    AAGTCTCAGC AGACGCTGCC CCCATCAGAG CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836831.1
    CDS471..1649
    Translation

    Target ORF information:

    RefSeq Version XM_024981063.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGCAG CGGGCGGCTC CCGGAACACG CTCCCAAGGC CCTGCCACGT GCTGGTGTTG 
    TTAAATCCGC GCGGGGGCAA GGGCAAAGCC CTGCAGCTCT TCAGGAGCCA CGTACAGCCG
    CTGCTGGCCC AGGCCGACGT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCC
    CGGGAGCTGG TGCGTGCGGA GGACCTGAGA CGCTGGGACG CGCTGGTGGT CATGTCCGGA
    GATGGGCTGA TCCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC
    ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG
    AACTATTACG CCGGGTACGA GCAGGTGACT AAGGAAGACC TCCTGACCAA CTGCACGCAG
    CTGCTGTGCC GCCGGCTGCT GGCGCCCATG GACCTGCTAT CCCTGCAGAC CGCCTGCGGG
    CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG TGGGGTTTCA TCGCCGACGT GGATCTGGAG
    AGCGAGAAGT TTCGGCGCCT GGGCGAGATG CGCTTCACTC TAGGCGCCTG CCTGCGCCTG
    GTGGCCCTGC GCACCTACCG GGGCAGCCTG GCCTACCTGC CCGCAGAAAC GCTGGCTTCC
    AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG CAGGACCCCG CGGACACCCA TGTGGTGCCC
    CTGGAGCAGC CAGTGCCTCC TCACTGGACG GTGGTGCCGG AGCAGGACTT TGTGCTGGTG
    TTAGCACTCC TGCATTCCCA CCTGGCCAGT GACCTTTTCA CCGCCCCCAT GGGCCGCTGT
    TCGGCTGGCG CCATGCATCT GTTCTACGTG CGTGCTGGCG TGTCCCGGGC CATGCTGCTG
    CGCCTCTTCC TGGCCATGGA GAAAGGCAGG CACATGGAGT GCCACTGCCC CCACCTGGTC
    TATGTGCCTG TGGTTGCCTT CCGCCTGGAG CCCAAGGATG GGAAGGGTGT GTTTACTGTG
    GATGGGGAAC TGCTGATCAG CGAGGCTGTC CAGGGCCAGG TTCTCCCCAA CTACTTCTGG
    ATGGTCAGTG GCTACAGGGA GCCCCCACCC TCTCTGAAAC CACAGCCCTC ACCCCGCAGG
    AAGTCTCAGC AGACGCTGCC CCCATCAGAG CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227034
    Clone ID Related Accession (Same CDS sequence) XM_024981063.1 , XM_024981064.1
    Accession Version XM_024981064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGCAG CGGGCGGCTC CCGGAACACG CTCCCAAGGC CCTGCCACGT GCTGGTGTTG 
    TTAAATCCGC GCGGGGGCAA GGGCAAAGCC CTGCAGCTCT TCAGGAGCCA CGTACAGCCG
    CTGCTGGCCC AGGCCGACGT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCC
    CGGGAGCTGG TGCGTGCGGA GGACCTGAGA CGCTGGGACG CGCTGGTGGT CATGTCCGGA
    GATGGGCTGA TCCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC
    ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG
    AACTATTACG CCGGGTACGA GCAGGTGACT AAGGAAGACC TCCTGACCAA CTGCACGCAG
    CTGCTGTGCC GCCGGCTGCT GGCGCCCATG GACCTGCTAT CCCTGCAGAC CGCCTGCGGG
    CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG TGGGGTTTCA TCGCCGACGT GGATCTGGAG
    AGCGAGAAGT TTCGGCGCCT GGGCGAGATG CGCTTCACTC TAGGCGCCTG CCTGCGCCTG
    GTGGCCCTGC GCACCTACCG GGGCAGCCTG GCCTACCTGC CCGCAGAAAC GCTGGCTTCC
    AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG CAGGACCCCG CGGACACCCA TGTGGTGCCC
    CTGGAGCAGC CAGTGCCTCC TCACTGGACG GTGGTGCCGG AGCAGGACTT TGTGCTGGTG
    TTAGCACTCC TGCATTCCCA CCTGGCCAGT GACCTTTTCA CCGCCCCCAT GGGCCGCTGT
    TCGGCTGGCG CCATGCATCT GTTCTACGTG CGTGCTGGCG TGTCCCGGGC CATGCTGCTG
    CGCCTCTTCC TGGCCATGGA GAAAGGCAGG CACATGGAGT GCCACTGCCC CCACCTGGTC
    TATGTGCCTG TGGTTGCCTT CCGCCTGGAG CCCAAGGATG GGAAGGGTGT GTTTACTGTG
    GATGGGGAAC TGCTGATCAG CGAGGCTGTC CAGGGCCAGG TTCTCCCCAA CTACTTCTGG
    ATGGTCAGTG GCTACAGGGA GCCCCCACCC TCTCTGAAAC CACAGCCCTC ACCCCGCAGG
    AAGTCTCAGC AGACGCTGCC CCCATCAGAG CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836832.1
    CDS438..1616
    Translation

    Target ORF information:

    RefSeq Version XM_024981064.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGATGCAG CGGGCGGCTC CCGGAACACG CTCCCAAGGC CCTGCCACGT GCTGGTGTTG 
    TTAAATCCGC GCGGGGGCAA GGGCAAAGCC CTGCAGCTCT TCAGGAGCCA CGTACAGCCG
    CTGCTGGCCC AGGCCGACGT CTCCTTCACG CTGATGCTCA CTGAGCGGCG GAACCACGCC
    CGGGAGCTGG TGCGTGCGGA GGACCTGAGA CGCTGGGACG CGCTGGTGGT CATGTCCGGA
    GATGGGCTGA TCCACGAGGT GGTGAACGGG CTCATGGAGC GGCCTGACTG GGAGACCGCC
    ATCCAGAAGC CCCTGTGTAG CCTCCCGGCA GGCTCTGGCA ATGCAGTGGC CGCTTCTTTG
    AACTATTACG CCGGGTACGA GCAGGTGACT AAGGAAGACC TCCTGACCAA CTGCACGCAG
    CTGCTGTGCC GCCGGCTGCT GGCGCCCATG GACCTGCTAT CCCTGCAGAC CGCCTGCGGG
    CAGCGCCTCT TCTCTGTGCT CAGCCTGGCG TGGGGTTTCA TCGCCGACGT GGATCTGGAG
    AGCGAGAAGT TTCGGCGCCT GGGCGAGATG CGCTTCACTC TAGGCGCCTG CCTGCGCCTG
    GTGGCCCTGC GCACCTACCG GGGCAGCCTG GCCTACCTGC CCGCAGAAAC GCTGGCTTCC
    AGGAGGGGCC CCTCCCCGGC TCTGGCCCCG CAGGACCCCG CGGACACCCA TGTGGTGCCC
    CTGGAGCAGC CAGTGCCTCC TCACTGGACG GTGGTGCCGG AGCAGGACTT TGTGCTGGTG
    TTAGCACTCC TGCATTCCCA CCTGGCCAGT GACCTTTTCA CCGCCCCCAT GGGCCGCTGT
    TCGGCTGGCG CCATGCATCT GTTCTACGTG CGTGCTGGCG TGTCCCGGGC CATGCTGCTG
    CGCCTCTTCC TGGCCATGGA GAAAGGCAGG CACATGGAGT GCCACTGCCC CCACCTGGTC
    TATGTGCCTG TGGTTGCCTT CCGCCTGGAG CCCAAGGATG GGAAGGGTGT GTTTACTGTG
    GATGGGGAAC TGCTGATCAG CGAGGCTGTC CAGGGCCAGG TTCTCCCCAA CTACTTCTGG
    ATGGTCAGTG GCTACAGGGA GCCCCCACCC TCTCTGAAAC CACAGCCCTC ACCCCGCAGG
    AAGTCTCAGC AGACGCTGCC CCCATCAGAG CGTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227031
    Clone ID Related Accession (Same CDS sequence) XM_024981053.1 , XM_024981051.1
    Accession Version XM_024981053.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTTGGCT CCCCAGTCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGG CGCCGCGTTT CCACCCGGGT GCCCGAGTTG TGATCCTCAG
    CCCCTAGTGG CGGTTCCGGT TTGTGTTTAC AGCGGGCGGC TCCCGGAACA CGCTCCCAAG
    GCCCTGCCAC GTGCTGGTGT TGTTAAATCC GCGCGGGGGC AAGGGCAAAG CCCTGCAGCT
    CTTCAGGAGC CACGTACAGC CGCTGCTGGC CCAGGCCGAC GTCTCCTTCA CGCTGATGCT
    CACTGCCGCG CCCTCTGCAC AGAGCGGCGG AACCACGCCC GGGAGCTGGT GCGTGCGGAG
    GACCTGAGAC GCTGGGACGC GCTGGTGGTC ATGTCCGGAG ATGGGCTGAT CCACGAGGTG
    GTGAACGGGC TCATGGAGCG GCCTGACTGG GAGACCGCCA TCCAGAAGCC CCTGTGTAGC
    CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC GCTTCTTTGA ACTATTACGC CGGGTACGAG
    CAGGTGACTA AGGAAGACCT CCTGACCAAC TGCACGCAGC TGCTGTGCCG CCGGCTGCTG
    GCGCCCATGG ACCTGCTATC CCTGCAGACC GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC
    AGCCTGGCGT GGGGTTTCAT CGCCGACGTG GATCTGGAGA GCGAGAAGTT TCGGCGCCTG
    GGCGAGATGC GCTTCACTCT AGGCGCCTGC CTGCGCCTGG TGGCCCTGCG CACCTACCGG
    GGCAGCCTGG CCTACCTGCC CGCAGAAACG CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT
    CTGGCCCCGC AGGACCCCGC GGACACCCAT GTGGTGCCCC TGGAGCAGCC AGTGCCTCCT
    CACTGGACGG TGGTGCCGGA GCAGGACTTT GTGCTGGTGT TAGCACTCCT GCATTCCCAC
    CTGGCCAGTG ACCTTTTCAC CGCCCCCATG GGCCGCTGTT CGGCTGGCGC CATGCATCTG
    TTCTACGTGC GTGCTGGCGT GTCCCGGGCC ATGCTGCTGC GCCTCTTCCT GGCCATGGAG
    AAAGGCAGGC ACATGGAGTG CCACTGCCCC CACCTGGTCT ATGTGCCTGT GGTTGCCTTC
    CGCCTGGAGC CCAAGGATGG GAAGGGTGTG TTTACTGTGG ATGGGGAACT GCTGATCAGC
    GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC TACTTCTGGA TGGTCAGTGG CTACAGGGAG
    CCCCCACCCT CTCTGAAACC ACAGCCCTCA CCCCGCAGGA AGTCTCAGCA GACGCTGCCC
    CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836821.1
    CDS244..1761
    Translation

    Target ORF information:

    RefSeq Version XM_024981053.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981053.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTTGGCT CCCCAGTCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGG CGCCGCGTTT CCACCCGGGT GCCCGAGTTG TGATCCTCAG
    CCCCTAGTGG CGGTTCCGGT TTGTGTTTAC AGCGGGCGGC TCCCGGAACA CGCTCCCAAG
    GCCCTGCCAC GTGCTGGTGT TGTTAAATCC GCGCGGGGGC AAGGGCAAAG CCCTGCAGCT
    CTTCAGGAGC CACGTACAGC CGCTGCTGGC CCAGGCCGAC GTCTCCTTCA CGCTGATGCT
    CACTGCCGCG CCCTCTGCAC AGAGCGGCGG AACCACGCCC GGGAGCTGGT GCGTGCGGAG
    GACCTGAGAC GCTGGGACGC GCTGGTGGTC ATGTCCGGAG ATGGGCTGAT CCACGAGGTG
    GTGAACGGGC TCATGGAGCG GCCTGACTGG GAGACCGCCA TCCAGAAGCC CCTGTGTAGC
    CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC GCTTCTTTGA ACTATTACGC CGGGTACGAG
    CAGGTGACTA AGGAAGACCT CCTGACCAAC TGCACGCAGC TGCTGTGCCG CCGGCTGCTG
    GCGCCCATGG ACCTGCTATC CCTGCAGACC GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC
    AGCCTGGCGT GGGGTTTCAT CGCCGACGTG GATCTGGAGA GCGAGAAGTT TCGGCGCCTG
    GGCGAGATGC GCTTCACTCT AGGCGCCTGC CTGCGCCTGG TGGCCCTGCG CACCTACCGG
    GGCAGCCTGG CCTACCTGCC CGCAGAAACG CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT
    CTGGCCCCGC AGGACCCCGC GGACACCCAT GTGGTGCCCC TGGAGCAGCC AGTGCCTCCT
    CACTGGACGG TGGTGCCGGA GCAGGACTTT GTGCTGGTGT TAGCACTCCT GCATTCCCAC
    CTGGCCAGTG ACCTTTTCAC CGCCCCCATG GGCCGCTGTT CGGCTGGCGC CATGCATCTG
    TTCTACGTGC GTGCTGGCGT GTCCCGGGCC ATGCTGCTGC GCCTCTTCCT GGCCATGGAG
    AAAGGCAGGC ACATGGAGTG CCACTGCCCC CACCTGGTCT ATGTGCCTGT GGTTGCCTTC
    CGCCTGGAGC CCAAGGATGG GAAGGGTGTG TTTACTGTGG ATGGGGAACT GCTGATCAGC
    GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC TACTTCTGGA TGGTCAGTGG CTACAGGGAG
    CCCCCACCCT CTCTGAAACC ACAGCCCTCA CCCCGCAGGA AGTCTCAGCA GACGCTGCCC
    CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227031
    Clone ID Related Accession (Same CDS sequence) XM_024981053.1 , XM_024981051.1
    Accession Version XM_024981051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1518bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sphingosine kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTTGGCT CCCCAGTCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGG CGCCGCGTTT CCACCCGGGT GCCCGAGTTG TGATCCTCAG
    CCCCTAGTGG CGGTTCCGGT TTGTGTTTAC AGCGGGCGGC TCCCGGAACA CGCTCCCAAG
    GCCCTGCCAC GTGCTGGTGT TGTTAAATCC GCGCGGGGGC AAGGGCAAAG CCCTGCAGCT
    CTTCAGGAGC CACGTACAGC CGCTGCTGGC CCAGGCCGAC GTCTCCTTCA CGCTGATGCT
    CACTGCCGCG CCCTCTGCAC AGAGCGGCGG AACCACGCCC GGGAGCTGGT GCGTGCGGAG
    GACCTGAGAC GCTGGGACGC GCTGGTGGTC ATGTCCGGAG ATGGGCTGAT CCACGAGGTG
    GTGAACGGGC TCATGGAGCG GCCTGACTGG GAGACCGCCA TCCAGAAGCC CCTGTGTAGC
    CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC GCTTCTTTGA ACTATTACGC CGGGTACGAG
    CAGGTGACTA AGGAAGACCT CCTGACCAAC TGCACGCAGC TGCTGTGCCG CCGGCTGCTG
    GCGCCCATGG ACCTGCTATC CCTGCAGACC GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC
    AGCCTGGCGT GGGGTTTCAT CGCCGACGTG GATCTGGAGA GCGAGAAGTT TCGGCGCCTG
    GGCGAGATGC GCTTCACTCT AGGCGCCTGC CTGCGCCTGG TGGCCCTGCG CACCTACCGG
    GGCAGCCTGG CCTACCTGCC CGCAGAAACG CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT
    CTGGCCCCGC AGGACCCCGC GGACACCCAT GTGGTGCCCC TGGAGCAGCC AGTGCCTCCT
    CACTGGACGG TGGTGCCGGA GCAGGACTTT GTGCTGGTGT TAGCACTCCT GCATTCCCAC
    CTGGCCAGTG ACCTTTTCAC CGCCCCCATG GGCCGCTGTT CGGCTGGCGC CATGCATCTG
    TTCTACGTGC GTGCTGGCGT GTCCCGGGCC ATGCTGCTGC GCCTCTTCCT GGCCATGGAG
    AAAGGCAGGC ACATGGAGTG CCACTGCCCC CACCTGGTCT ATGTGCCTGT GGTTGCCTTC
    CGCCTGGAGC CCAAGGATGG GAAGGGTGTG TTTACTGTGG ATGGGGAACT GCTGATCAGC
    GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC TACTTCTGGA TGGTCAGTGG CTACAGGGAG
    CCCCCACCCT CTCTGAAACC ACAGCCCTCA CCCCGCAGGA AGTCTCAGCA GACGCTGCCC
    CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836819.1
    CDS603..2120
    Translation

    Target ORF information:

    RefSeq Version XM_024981051.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTTGGCT CCCCAGTCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGG CGCCGCGTTT CCACCCGGGT GCCCGAGTTG TGATCCTCAG
    CCCCTAGTGG CGGTTCCGGT TTGTGTTTAC AGCGGGCGGC TCCCGGAACA CGCTCCCAAG
    GCCCTGCCAC GTGCTGGTGT TGTTAAATCC GCGCGGGGGC AAGGGCAAAG CCCTGCAGCT
    CTTCAGGAGC CACGTACAGC CGCTGCTGGC CCAGGCCGAC GTCTCCTTCA CGCTGATGCT
    CACTGCCGCG CCCTCTGCAC AGAGCGGCGG AACCACGCCC GGGAGCTGGT GCGTGCGGAG
    GACCTGAGAC GCTGGGACGC GCTGGTGGTC ATGTCCGGAG ATGGGCTGAT CCACGAGGTG
    GTGAACGGGC TCATGGAGCG GCCTGACTGG GAGACCGCCA TCCAGAAGCC CCTGTGTAGC
    CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC GCTTCTTTGA ACTATTACGC CGGGTACGAG
    CAGGTGACTA AGGAAGACCT CCTGACCAAC TGCACGCAGC TGCTGTGCCG CCGGCTGCTG
    GCGCCCATGG ACCTGCTATC CCTGCAGACC GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC
    AGCCTGGCGT GGGGTTTCAT CGCCGACGTG GATCTGGAGA GCGAGAAGTT TCGGCGCCTG
    GGCGAGATGC GCTTCACTCT AGGCGCCTGC CTGCGCCTGG TGGCCCTGCG CACCTACCGG
    GGCAGCCTGG CCTACCTGCC CGCAGAAACG CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT
    CTGGCCCCGC AGGACCCCGC GGACACCCAT GTGGTGCCCC TGGAGCAGCC AGTGCCTCCT
    CACTGGACGG TGGTGCCGGA GCAGGACTTT GTGCTGGTGT TAGCACTCCT GCATTCCCAC
    CTGGCCAGTG ACCTTTTCAC CGCCCCCATG GGCCGCTGTT CGGCTGGCGC CATGCATCTG
    TTCTACGTGC GTGCTGGCGT GTCCCGGGCC ATGCTGCTGC GCCTCTTCCT GGCCATGGAG
    AAAGGCAGGC ACATGGAGTG CCACTGCCCC CACCTGGTCT ATGTGCCTGT GGTTGCCTTC
    CGCCTGGAGC CCAAGGATGG GAAGGGTGTG TTTACTGTGG ATGGGGAACT GCTGATCAGC
    GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC TACTTCTGGA TGGTCAGTGG CTACAGGGAG
    CCCCCACCCT CTCTGAAACC ACAGCCCTCA CCCCGCAGGA AGTCTCAGCA GACGCTGCCC
    CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30451
    Clone ID Related Accession (Same CDS sequence) XM_002696204.5 , XM_005195854.3
    Accession Version XM_002696204.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: sphingosine kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104499.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696204.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTTGGCT CCCCAATCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGC GGGCGGCTCC CGGAACACGC TCCCAAGGCC CTGCCACGTG
    CTGGTGTTGT TAAATCCGCG CGGGGGCAAG GGCAAAGCCC TGCAGCTCTT CAGGAGCCAC
    GTACAGCCGC TGCTGGCCCA GGCCGACGTC TCCTTCACGC TGATGCTCAC TGAGCGGCGG
    AACCACGCCC GGGAGCTGGT GCGTGCGGAG GACCTGAGAC GCTGGGACGC GCTGGTGGTC
    ATGTCCGGAG ATGGGCTGAT CCACGAGGTG GTGAACGGGC TCATGGAGCG GCCTGACTGG
    GAGACCGCCA TCCAGAAGCC CCTGTGTAGC CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC
    GCTTCTTTGA ACTATTACGC CGGGTACGAG CAGGTGACTA AGGAAGACCT CCTGACCAAC
    TGCACGCAGC TGCTGTGCCG CCGGCTGCTG GCGCCCATGG ACCTGCTATC CCTGCAGACC
    GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC AGCCTGGCGT GGGGTTTCAT CGCCGACGTG
    GATCTGGAGA GCGAGAAGTT TCGGCGCCTG GGCGAGATGC GCTTCACTCT AGGCGCCTGC
    CTGCGCCTGG TGGCCCTGCG CACCTACCGG GGCAGCCTGG CCTACCTGCC CGCAGAAACG
    CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT CTGGCCCCGC AGGACCCCGC GGACACCCAT
    GTGGTGCCCN TGGAGCAGCC AGTGCCTCCT CACTGGACGG TGGTGCCGGA GCAGGACTTT
    GTGCTGGTGT TAGCACTCCT GCATTCCCAC CTGGCCAGTG ACCTTTTCAC CGCCCCCATG
    GGCCGCTGTT CGGCTGGCGC CATGCATCTG TTCTACGTGC GTGCTGGCGT GTCCCGGGCC
    ATGCTGCTGC GCCTCTTCCT GGCCATGGAG AAAGGCAGGC ACATGGAGTG CCACTGCCCC
    CACCTGGTCT ATGTGCCTGT GGTTGCCTTC CGCCTGGAGC CCAAGGATGG GAAGGGTGTG
    TTTACTGTGG ATGGGGAACT GCTGATCAGC GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC
    TACTTCTGGA TGGTCAGTGG CTACAGAGAG CCCCCACCCT CTCTGAAACC ACAGCCCTCA
    CCCCGCAGGA AGTCTCAGCA GACGCTGCCC CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696250.3
    CDS1121..2548
    Translation

    Target ORF information:

    RefSeq Version XM_002696204.5
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696204.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTTGGCT CCCCAATCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGC GGGCGGCTCC CGGAACACGC TCCCAAGGCC CTGCCACGTG
    CTGGTGTTGT TAAATCCGCG CGGGGGCAAG GGCAAAGCCC TGCAGCTCTT CAGGAGCCAC
    GTACAGCCGC TGCTGGCCCA GGCCGACGTC TCCTTCACGC TGATGCTCAC TGAGCGGCGG
    AACCACGCCC GGGAGCTGGT GCGTGCGGAG GACCTGAGAC GCTGGGACGC GCTGGTGGTC
    ATGTCCGGAG ATGGGCTGAT CCACGAGGTG GTGAACGGGC TCATGGAGCG GCCTGACTGG
    GAGACCGCCA TCCAGAAGCC CCTGTGTAGC CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC
    GCTTCTTTGA ACTATTACGC CGGGTACGAG CAGGTGACTA AGGAAGACCT CCTGACCAAC
    TGCACGCAGC TGCTGTGCCG CCGGCTGCTG GCGCCCATGG ACCTGCTATC CCTGCAGACC
    GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC AGCCTGGCGT GGGGTTTCAT CGCCGACGTG
    GATCTGGAGA GCGAGAAGTT TCGGCGCCTG GGCGAGATGC GCTTCACTCT AGGCGCCTGC
    CTGCGCCTGG TGGCCCTGCG CACCTACCGG GGCAGCCTGG CCTACCTGCC CGCAGAAACG
    CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT CTGGCCCCGC AGGACCCCGC GGACACCCAT
    GTGGTGCCCN TGGAGCAGCC AGTGCCTCCT CACTGGACGG TGGTGCCGGA GCAGGACTTT
    GTGCTGGTGT TAGCACTCCT GCATTCCCAC CTGGCCAGTG ACCTTTTCAC CGCCCCCATG
    GGCCGCTGTT CGGCTGGCGC CATGCATCTG TTCTACGTGC GTGCTGGCGT GTCCCGGGCC
    ATGCTGCTGC GCCTCTTCCT GGCCATGGAG AAAGGCAGGC ACATGGAGTG CCACTGCCCC
    CACCTGGTCT ATGTGCCTGT GGTTGCCTTC CGCCTGGAGC CCAAGGATGG GAAGGGTGTG
    TTTACTGTGG ATGGGGAACT GCTGATCAGC GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC
    TACTTCTGGA TGGTCAGTGG CTACAGAGAG CCCCCACCCT CTCTGAAACC ACAGCCCTCA
    CCCCGCAGGA AGTCTCAGCA GACGCTGCCC CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30451
    Clone ID Related Accession (Same CDS sequence) XM_002696204.5 , XM_005195854.3
    Accession Version XM_005195854.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: sphingosine kinase 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646467.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTTGGCT CCCCAATCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGC GGGCGGCTCC CGGAACACGC TCCCAAGGCC CTGCCACGTG
    CTGGTGTTGT TAAATCCGCG CGGGGGCAAG GGCAAAGCCC TGCAGCTCTT CAGGAGCCAC
    GTACAGCCGC TGCTGGCCCA GGCCGACGTC TCCTTCACGC TGATGCTCAC TGAGCGGCGG
    AACCACGCCC GGGAGCTGGT GCGTGCGGAG GACCTGAGAC GCTGGGACGC GCTGGTGGTC
    ATGTCCGGAG ATGGGCTGAT CCACGAGGTG GTGAACGGGC TCATGGAGCG GCCTGACTGG
    GAGACCGCCA TCCAGAAGCC CCTGTGTAGC CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC
    GCTTCTTTGA ACTATTACGC CGGGTACGAG CAGGTGACTA AGGAAGACCT CCTGACCAAC
    TGCACGCAGC TGCTGTGCCG CCGGCTGCTG GCGCCCATGG ACCTGCTATC CCTGCAGACC
    GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC AGCCTGGCGT GGGGTTTCAT CGCCGACGTG
    GATCTGGAGA GCGAGAAGTT TCGGCGCCTG GGCGAGATGC GCTTCACTCT AGGCGCCTGC
    CTGCGCCTGG TGGCCCTGCG CACCTACCGG GGCAGCCTGG CCTACCTGCC CGCAGAAACG
    CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT CTGGCCCCGC AGGACCCCGC GGACACCCAT
    GTGGTGCCCN TGGAGCAGCC AGTGCCTCCT CACTGGACGG TGGTGCCGGA GCAGGACTTT
    GTGCTGGTGT TAGCACTCCT GCATTCCCAC CTGGCCAGTG ACCTTTTCAC CGCCCCCATG
    GGCCGCTGTT CGGCTGGCGC CATGCATCTG TTCTACGTGC GTGCTGGCGT GTCCCGGGCC
    ATGCTGCTGC GCCTCTTCCT GGCCATGGAG AAAGGCAGGC ACATGGAGTG CCACTGCCCC
    CACCTGGTCT ATGTGCCTGT GGTTGCCTTC CGCCTGGAGC CCAAGGATGG GAAGGGTGTG
    TTTACTGTGG ATGGGGAACT GCTGATCAGC GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC
    TACTTCTGGA TGGTCAGTGG CTACAGAGAG CCCCCACCCT CTCTGAAACC ACAGCCCTCA
    CCCCGCAGGA AGTCTCAGCA GACGCTGCCC CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195911.2
    CDS1121..2548
    Translation

    Target ORF information:

    RefSeq Version XM_005195854.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sphingosine kinase 1 (SPHK1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195854.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGCTTGGCT CCCCAATCCG GATGGGGTCG CCTAGGCCTG TTTACACCGC CAAGCTCCTT 
    TCAGATCCGG CAACCGCAGG GAATGACGCG GGTGCCCCCA CAGCCCCGGC AACGGGCGGG
    GAGGGCGAGC CCCATAGCCG TCACCGAGAG GCCCGCCTGG GCAGCACCGA TAAGGAGCTG
    AAGTCAGGAG CCGCCGCGGC GGACAGCGTC CGGACGGCGC CGGGGACCCC TCGGCAGCGG
    GGCGAGGTCA TGGATGCAGC GGGCGGCTCC CGGAACACGC TCCCAAGGCC CTGCCACGTG
    CTGGTGTTGT TAAATCCGCG CGGGGGCAAG GGCAAAGCCC TGCAGCTCTT CAGGAGCCAC
    GTACAGCCGC TGCTGGCCCA GGCCGACGTC TCCTTCACGC TGATGCTCAC TGAGCGGCGG
    AACCACGCCC GGGAGCTGGT GCGTGCGGAG GACCTGAGAC GCTGGGACGC GCTGGTGGTC
    ATGTCCGGAG ATGGGCTGAT CCACGAGGTG GTGAACGGGC TCATGGAGCG GCCTGACTGG
    GAGACCGCCA TCCAGAAGCC CCTGTGTAGC CTCCCGGCAG GCTCTGGCAA TGCAGTGGCC
    GCTTCTTTGA ACTATTACGC CGGGTACGAG CAGGTGACTA AGGAAGACCT CCTGACCAAC
    TGCACGCAGC TGCTGTGCCG CCGGCTGCTG GCGCCCATGG ACCTGCTATC CCTGCAGACC
    GCCTGCGGGC AGCGCCTCTT CTCTGTGCTC AGCCTGGCGT GGGGTTTCAT CGCCGACGTG
    GATCTGGAGA GCGAGAAGTT TCGGCGCCTG GGCGAGATGC GCTTCACTCT AGGCGCCTGC
    CTGCGCCTGG TGGCCCTGCG CACCTACCGG GGCAGCCTGG CCTACCTGCC CGCAGAAACG
    CTGGCTTCCA GGAGGGGCCC CTCCCCGGCT CTGGCCCCGC AGGACCCCGC GGACACCCAT
    GTGGTGCCCN TGGAGCAGCC AGTGCCTCCT CACTGGACGG TGGTGCCGGA GCAGGACTTT
    GTGCTGGTGT TAGCACTCCT GCATTCCCAC CTGGCCAGTG ACCTTTTCAC CGCCCCCATG
    GGCCGCTGTT CGGCTGGCGC CATGCATCTG TTCTACGTGC GTGCTGGCGT GTCCCGGGCC
    ATGCTGCTGC GCCTCTTCCT GGCCATGGAG AAAGGCAGGC ACATGGAGTG CCACTGCCCC
    CACCTGGTCT ATGTGCCTGT GGTTGCCTTC CGCCTGGAGC CCAAGGATGG GAAGGGTGTG
    TTTACTGTGG ATGGGGAACT GCTGATCAGC GAGGCTGTCC AGGGCCAGGT TCTCCCCAAC
    TACTTCTGGA TGGTCAGTGG CTACAGAGAG CCCCCACCCT CTCTGAAACC ACAGCCCTCA
    CCCCGCAGGA AGTCTCAGCA GACGCTGCCC CCATCAGAGC GTCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.