FGF4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	FGF4
Entrez Gene ID	618474
Full Name	fibroblast growth factor 4
Synonyms	HBGF-4,HST
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040605.2	NP_001035695.2	fibroblast growth factor 4 precursor

Pathways from BioSystems

Homologies

	FGF4	XP_001100747.1
Xenopus tropicalis (tropical clawed frog)	fgf4	NP_001136294.1
Homo sapiens (human)	FGF4	NP_001998.1
Bos taurus (cattle)	FGF4	NP_001035695.2
Danio rerio (zebrafish)	fgf4	NP_571710.1
Pan troglodytes (chimpanzee)	FGF4	XP_001173970.1
Canis lupus familiaris (dog)	FGF4	XP_540801.2
Mus musculus (house mouse)	Fgf4	NP_034332.2
Rattus norvegicus (Norway rat)	Fgf4	NP_446261.1
Gallus gallus (chicken)	FGF4	NP_001026717.1

Gene Ontology
Bibliography

Related articles in PubMed

Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds.
Sugawara S, Ito T, Sato S, Yokoo M, Mori Y, Kasuga K, Kojima I, Fukuda T, Yamanaka K, Sakatani M, Takahashi M, Kobayashi M
Animal science journal = Nihon chikusan Gakkaiho84(3)275-80(2013 Mar)

Efficient derivation of bovine embryonic stem cells needs more than active core pluripotency factors.
Maruotti J, Mu?oz M, Degrelle SA, G?mez E, Louet C, D?ez C, Monforte CD, de Longchamp PH, Brochard V, Hue I, Caama?o JN, Jouneau A
Molecular reproduction and development79(7)461-77(2012 Jul)

Common nucleotide sequence of structural gene encoding fibroblast growth factor 4 in eight cattle derived from three breeds.
Sato S, Takahashi T, Nishinomiya H, Katoh M, Itoh R, Yokoo M, Yokoo M, Iha M, Mori Y, Kasuga K, Kojima I, Kobayashi M
Animal science journal = Nihon chikusan Gakkaiho83(3)260-2(2012 Mar)

Methylation patterns in 5' terminal regions of pluripotency-related genes in mature bovine gametes.
Lan J, Hua S, Yuan Y, Zhan L, He X, Zhang Y
Zygote (Cambridge, England)19(2)165-9(2011 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HisbFGF4, a 6x histidine-tagged bovine FGF4 (Pro(32) -Leu(206) ), was produced in Escherichia coli based on the validated nucleotide sequence and purified by heparin column chromatography.
Title: Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds.

This is the first determination of the complete nucleotide sequence of the structural gene encoding bovine FGF4 in identified breeds.
Title: Common nucleotide sequence of structural gene encoding fibroblast growth factor 4 in eight cattle derived from three breeds.

Fgf4 exhibited significant hypermethylation in sperm compared with that in oocytes
Title: Methylation patterns in 5' terminal regions of pluripotency-related genes in mature bovine gametes.

The following FGF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44649	NM_001040605.2 Latest version!	Bos taurus fibroblast growth factor 4 (FGF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FGF4 ( NM_001040605.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001035695.2 Bos taurus fibroblast growth factor 4 (FGF4), mRNA.

Price & Availability↑

CloneID	OBa44649
Clone ID Related Accession (Same CDS sequence)	NM_001040605.2
Accession Version	NM_001040605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Bos taurus(cattle)
Product	fibroblast growth factor 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB633206.1. On Mar 27, 2012 this sequence version replaced NM_001040605.1.

ATGGCGGGGC CCGGGGCGGC CGCGGCGGCG CTGCTCCCGG CGGTGCTGCT GGCCGTGCTG 
GCGCCCTGGG CCGGCCGAGG GGGCGCCGCC GCGCCCACCG CCCCCAACGG CACGCTGGAG 
GCCGAGCTGG AGCGCCGCTG GGAGAGCCTG GTGGCGCGCT CGCTGGCGCG CCTGCCGGTG 
GCCGCGCAGC CCAAGGAGGC TGCCGTCCAG AGCGGCGCCG GCGACTACTT GCTGGGCATC 
AAGCGGTTGC GGAGGCTGTA CTGCAACGTG GGCATCGGCT TCCACCTCCA GGTGCTCCCC 
GACGGCCGCA TCGGCGGCGT GCACGCGGAC ACGAGTGACA GCCTGCTGGA GCTGTCGCCG 
GTGGAGCGCG GGGTGGTGAG CATTTTCGGG GTGGCCAGCC GGTTCTTCGT GGCCATGAGC 
AGCCGGGGCA GGCTGTACGG CTCGCCTTTC TTCACCGACG AGTGCAGGTT CAGAGAGATC 
CTCCTCCCCA ACAACTACAA CGCCTACGAG TGCGACCGGC ACCCCGGCAT GTTCATCGCC 
CTGAGCAAGA ACGGCAAGGC CAAGAAGGGG AACCGGGTGT CCCCCACCAT GAAGGTCACC 
CACTTCCTCC CCAGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035695.2
CDS	1..621
Translation	MAGPGAAAAALLPAVLLAVLAPWAGRGGAAAPTAPNGTLEAELERRWESLVARSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQVLPDGRIGGVHADTSDSLLELSPVERGVVSIFGVASRFFVAMSSRGRLYGSPFFTDECRFREILLPNNYNAYECDRHPGMFIALSKNGKAKKGNRVSPTMKVTHFLPRL

Target ORF information:

RefSeq Version	NM_001040605.2
Organism	Bos taurus(cattle)
Definition	Bos taurus fibroblast growth factor 4 (FGF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040605.2

ORF Insert Sequence:

ATGGCGGGGC CCGGGGCGGC CGCGGCGGCG CTGCTCCCGG CGGTGCTGCT GGCCGTGCTG 
GCGCCCTGGG CCGGCCGAGG GGGCGCCGCC GCGCCCACCG CCCCCAACGG CACGCTGGAG 
GCCGAGCTGG AGCGCCGCTG GGAGAGCCTG GTGGCGCGCT CGCTGGCGCG CCTGCCGGTG 
GCCGCGCAGC CCAAGGAGGC TGCCGTCCAG AGCGGCGCCG GCGACTACTT GCTGGGCATC 
AAGCGGTTGC GGAGGCTGTA CTGCAACGTG GGCATCGGCT TCCACCTCCA GGTGCTCCCC 
GACGGCCGCA TCGGCGGCGT GCACGCGGAC ACGAGTGACA GCCTGCTGGA GCTGTCGCCG 
GTGGAGCGCG GGGTGGTGAG CATTTTCGGG GTGGCCAGCC GGTTCTTCGT GGCCATGAGC 
AGCCGGGGCA GGCTGTACGG CTCGCCTTTC TTCACCGACG AGTGCAGGTT CAGAGAGATC 
CTCCTCCCCA ACAACTACAA CGCCTACGAG TGCGACCGGC ACCCCGGCAT GTTCATCGCC 
CTGAGCAAGA ACGGCAAGGC CAAGAAGGGG AACCGGGTGT CCCCCACCAT GAAGGTCACC 
CACTTCCTCC CCAGGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Production of bioactive bovine fibroblast growth factor 4 in E. coli based on the common nucleotide sequence of its structural gene in three breeds.
Animal science journal = Nihon chikusan Gakkaiho84(3)275-80(2013 Mar)
Sugawara S,Ito T,Sato S,Yokoo M,Mori Y,Kasuga K,Kojima I,Fukuda T,Yamanaka K,Sakatani M,Takahashi M,Kobayashi M

Efficient derivation of bovine embryonic stem cells needs more than active core pluripotency factors.
Molecular reproduction and development79(7)461-77(2012 Jul)
Maruotti J,Mu?oz M,Degrelle SA,G?mez E,Louet C,D?ez C,Monforte CD,de Longchamp PH,Brochard V,Hue I,Caama?o JN,Jouneau A

Common nucleotide sequence of structural gene encoding fibroblast growth factor 4 in eight cattle derived from three breeds.
Animal science journal = Nihon chikusan Gakkaiho83(3)260-2(2012 Mar)
Sato S,Takahashi T,Nishinomiya H,Katoh M,Itoh R,Yokoo M,Yokoo M,Iha M,Mori Y,Kasuga K,Kojima I,Kobayashi M

Methylation patterns in 5' terminal regions of pluripotency-related genes in mature bovine gametes.
Zygote (Cambridge, England)19(2)165-9(2011 May)
Lan J,Hua S,Yuan Y,Zhan L,He X,Zhang Y

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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FGF4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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