PROM1 cDNA ORF clone, Bos taurus(cattle)

The following PROM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PROM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa20696 XM_010806398.2
Latest version!
Bos taurus prominin 1 (PROM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
OBa20697 XM_010806399.2
Latest version!
Bos taurus prominin 1 (PROM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $510.30
$729.00
OBa38064 NM_001245952.2
Latest version!
Bos taurus prominin 1 (PROM1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa20695 XM_005208330.3
Latest version!
Bos taurus prominin 1 (PROM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa20699 XM_005208337.3
Latest version!
Bos taurus prominin 1 (PROM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa235444 XM_024993438.1
Latest version!
Bos taurus prominin 1 (PROM1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa90973 XM_010806402.2
Latest version!
Bos taurus prominin 1 (PROM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa90974 XM_015471778.1
Latest version!
Bos taurus prominin 1 (PROM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa20696
    Clone ID Related Accession (Same CDS sequence) XM_010806398.2
    Accession Version XM_010806398.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2613bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product prominin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806398.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGATTA TCTACTATGA GATCGGGATT
    ATTATCTGTG CTGCCCTGGG ATTGCTGTTT GTTATTCTGA TGCCTCTGGT GGGCTTTTTC
    TTTTGTCTGT GTCGTTGCTG TAACAAGTGT GGTGGAGAAA TGCACCAGCG ACAGAAGAAA
    AGTGGTCCCT TCCTCAAGAA ATACTTTACA ATCTCCCTCC TGGTGATCTG TGTGTTCATA
    AGCATCGGGA TCATCTATGG TTTTGCCGCA AATCATTACA TGCGGACTAA CGTGGAAGAG
    ACTCGGAAAT TGTCAGAAAG TAACTTAAAC GACCTGAGAA CTCTCCTGAA TGTGGTGCCA
    GGGCAAATTG ACTACATACT GGACCAGTTT ACCCTGCCTA AGGAGAAAGC ATTTGACGAT
    CTGGACAATA TTAACTTGTT GGTGGGAGGC AGCATTTACG AGCGTCTCAA ACCCAAAGTG
    CTCCCTGTGC TGAAGGACAT CAAGGACTTG GCAGAAGACA TGAAAACTAA TAGAGATACC
    CTGGTCCGAA TGAACACTGT CTTAACGGAC ATGAAGCAGT CATCTGCACA ACTTCGCACC
    AGCCTGAGAG ACGTGAAAAC GAACATGGAG CAAACACTCA TGGATCCCCA GTGCTCCTCA
    CCTGCAGCGG CGCCCACGTG CGACAGCATC AGGAAGTCTC TGAGCGTCCT GGATGGCAGT
    GCCAATTTTG ATCACCTCCC GTCTTTGGAT GGACACATTA CTCAGCTTGA TGGACTTCTT
    CAAACAGATT TGTCCGGCCT GGTCCAAAAG GCCAATGAAT CTCTTTCTAA TATACCTGAG
    GAAGTGCAAA ATCAAACCAG GGACTTCATA TCAGAGTTTA AAAAGACCTT GAATTCCCTT
    CAATCTGATG TCAAAAATAT AAGCACGAAG ATTCCTATTC AGAAGACACT GTCGAATTTC
    GTCCGTTACA TTAATGACTC TGAAGATTAT ATCCTCCAGT ATTTACCCAC TATGGAAGAA
    TGCGATTCAT ACAGGTGGCT GGTTTGCTTG GTCATCTGCT GCTTGCTGAC CCTCATCTTG
    ATTTTTTACC TCCTGGGCTT GCTCTGTGGC ACGTTGGGCT ATGACCAGAA AGCCACCCCA
    ACCAGACGAG GCTGCGTCTC GAACACCGGG GGCCTCCTCC TCATGGTTGG GGTTGGACTC
    AGTTTCTTCT TTTCTTGGAT AATCATGACC ATCGTGGTGC TGACTTTTGT CACGGGTGGA
    AATATGGAAA AACTGGTCTG TGAGCCTTAC AGAAACAAAA AGTTATTCCA GGTTTTGGAC
    ACTCCATATT TACTAAATGA GGATTGGAAA TACTATCTCT CTGGCCTAGT ATTTAACAAA
    CCAGATATTA ATCTCACTTT TGAACAAGTT TACAGTGACT GCAAAGAAAA TAAAGGCCTT
    TATGCCACAC TTAAGCTCGA CCACATCTAC AATGTCAGTG AACAGCTCAA CATTACAAAG
    CATACTGGAG ACATAAATAG TAATTTGGAA AATATGAATA TAAGAATTGA GGATATAGAA
    CTGCTGGATA AAACAGGAAT GAAAACCCTT ATGGATCTCA GATCTTCTGG AATAGACGAT
    ATAGACTATG CTGCCTACTT GAACGCGACC GAGAGAAGTC CCACGAGAGT AAATCTTATC
    TCATTTGCAA ATAATCTCCG AAGAAAAGCC AACCAGCTGC CCTCAGGAAA CTTGAAAACC
    AGCTTGAAAA GTCACACAGA TACCTTAACA AATATCCATG AGAACCAGGT CGTTCCTCTG
    CAGAATTCAA TGAACGCCAT GCACCAGCAA ATGAAGGGGC TTCAGTACAG GACCAGTGGA
    CTGAAGGTCA GAGTGTCCAC CACCATTTTC TTTTTGAATT CTACCCAGGA CTTTCTCACC
    AGCCAGCTGT CCGAAGTTGT TGTTGAAGAA AGTAAGCAGT TCGGGAATAA GATAATAAGC
    TACTTTGAAC GTTATCTGCA GTGGGTCGAG ATGGCCATCA CCCAGCAATT TGCAGCCTGC
    AAACCGGCGG CCACTGCTCT GGATTCGGCT GTTAATGTCT TTCTGTGCAG CTACATCGTC
    GACCCCCTGA ATCTGTTTTG GTTTGGCGTG GGAAAAGCGA CCATACTTTT ACTTCCTGCT
    GTCATTTTTG CCGTGAAGCT GGCTAAGTAC TTCCGTCGAA TGTATTCTGA AGATGTGTAT
    GAGGATGAGC CAGTGAACAA GGTTCAAAGT AAACCCAGGG CTCAAACTGT ACCCAGGGTT
    CAAACTGTAC CCATGGTTCA AACTGTACCC ATGACAAATA TGAGACATGC TAATACCCGT
    TCTCGTGTAG AACAAGGCAC ACAGACAATT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804700.1
    CDS467..3079
    Translation

    Target ORF information:

    RefSeq Version XM_010806398.2
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806398.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGATTA TCTACTATGA GATCGGGATT
    ATTATCTGTG CTGCCCTGGG ATTGCTGTTT GTTATTCTGA TGCCTCTGGT GGGCTTTTTC
    TTTTGTCTGT GTCGTTGCTG TAACAAGTGT GGTGGAGAAA TGCACCAGCG ACAGAAGAAA
    AGTGGTCCCT TCCTCAAGAA ATACTTTACA ATCTCCCTCC TGGTGATCTG TGTGTTCATA
    AGCATCGGGA TCATCTATGG TTTTGCCGCA AATCATTACA TGCGGACTAA CGTGGAAGAG
    ACTCGGAAAT TGTCAGAAAG TAACTTAAAC GACCTGAGAA CTCTCCTGAA TGTGGTGCCA
    GGGCAAATTG ACTACATACT GGACCAGTTT ACCCTGCCTA AGGAGAAAGC ATTTGACGAT
    CTGGACAATA TTAACTTGTT GGTGGGAGGC AGCATTTACG AGCGTCTCAA ACCCAAAGTG
    CTCCCTGTGC TGAAGGACAT CAAGGACTTG GCAGAAGACA TGAAAACTAA TAGAGATACC
    CTGGTCCGAA TGAACACTGT CTTAACGGAC ATGAAGCAGT CATCTGCACA ACTTCGCACC
    AGCCTGAGAG ACGTGAAAAC GAACATGGAG CAAACACTCA TGGATCCCCA GTGCTCCTCA
    CCTGCAGCGG CGCCCACGTG CGACAGCATC AGGAAGTCTC TGAGCGTCCT GGATGGCAGT
    GCCAATTTTG ATCACCTCCC GTCTTTGGAT GGACACATTA CTCAGCTTGA TGGACTTCTT
    CAAACAGATT TGTCCGGCCT GGTCCAAAAG GCCAATGAAT CTCTTTCTAA TATACCTGAG
    GAAGTGCAAA ATCAAACCAG GGACTTCATA TCAGAGTTTA AAAAGACCTT GAATTCCCTT
    CAATCTGATG TCAAAAATAT AAGCACGAAG ATTCCTATTC AGAAGACACT GTCGAATTTC
    GTCCGTTACA TTAATGACTC TGAAGATTAT ATCCTCCAGT ATTTACCCAC TATGGAAGAA
    TGCGATTCAT ACAGGTGGCT GGTTTGCTTG GTCATCTGCT GCTTGCTGAC CCTCATCTTG
    ATTTTTTACC TCCTGGGCTT GCTCTGTGGC ACGTTGGGCT ATGACCAGAA AGCCACCCCA
    ACCAGACGAG GCTGCGTCTC GAACACCGGG GGCCTCCTCC TCATGGTTGG GGTTGGACTC
    AGTTTCTTCT TTTCTTGGAT AATCATGACC ATCGTGGTGC TGACTTTTGT CACGGGTGGA
    AATATGGAAA AACTGGTCTG TGAGCCTTAC AGAAACAAAA AGTTATTCCA GGTTTTGGAC
    ACTCCATATT TACTAAATGA GGATTGGAAA TACTATCTCT CTGGCCTAGT ATTTAACAAA
    CCAGATATTA ATCTCACTTT TGAACAAGTT TACAGTGACT GCAAAGAAAA TAAAGGCCTT
    TATGCCACAC TTAAGCTCGA CCACATCTAC AATGTCAGTG AACAGCTCAA CATTACAAAG
    CATACTGGAG ACATAAATAG TAATTTGGAA AATATGAATA TAAGAATTGA GGATATAGAA
    CTGCTGGATA AAACAGGAAT GAAAACCCTT ATGGATCTCA GATCTTCTGG AATAGACGAT
    ATAGACTATG CTGCCTACTT GAACGCGACC GAGAGAAGTC CCACGAGAGT AAATCTTATC
    TCATTTGCAA ATAATCTCCG AAGAAAAGCC AACCAGCTGC CCTCAGGAAA CTTGAAAACC
    AGCTTGAAAA GTCACACAGA TACCTTAACA AATATCCATG AGAACCAGGT CGTTCCTCTG
    CAGAATTCAA TGAACGCCAT GCACCAGCAA ATGAAGGGGC TTCAGTACAG GACCAGTGGA
    CTGAAGGTCA GAGTGTCCAC CACCATTTTC TTTTTGAATT CTACCCAGGA CTTTCTCACC
    AGCCAGCTGT CCGAAGTTGT TGTTGAAGAA AGTAAGCAGT TCGGGAATAA GATAATAAGC
    TACTTTGAAC GTTATCTGCA GTGGGTCGAG ATGGCCATCA CCCAGCAATT TGCAGCCTGC
    AAACCGGCGG CCACTGCTCT GGATTCGGCT GTTAATGTCT TTCTGTGCAG CTACATCGTC
    GACCCCCTGA ATCTGTTTTG GTTTGGCGTG GGAAAAGCGA CCATACTTTT ACTTCCTGCT
    GTCATTTTTG CCGTGAAGCT GGCTAAGTAC TTCCGTCGAA TGTATTCTGA AGATGTGTAT
    GAGGATGAGC CAGTGAACAA GGTTCAAAGT AAACCCAGGG CTCAAACTGT ACCCAGGGTT
    CAAACTGTAC CCATGGTTCA AACTGTACCC ATGACAAATA TGAGACATGC TAATACCCGT
    TCTCGTGTAG AACAAGGCAC ACAGACAATT TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20697
    Clone ID Related Accession (Same CDS sequence) XM_010806399.2
    Accession Version XM_010806399.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2595bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product prominin-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTAAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTGCTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAA TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT CTGTGGCACG
    TTGGGCTATG ACCAGAAAGC CACCCCAACC AGACGAGGCT GCGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGACTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCAAC
    CAGCTGCCCT CAGGAAACTT GAAAACCAGC TTGAAAAGTC ACACAGATAC CTTAACAAAT
    ATCCATGAGA ACCAGGTCGT TCCTCTGCAG AATTCAATGA ACGCCATGCA CCAGCAAATG
    AAGGGGCTTC AGTACAGGAC CAGTGGACTG AAGGTCAGAG TGTCCACCAC CATTTTCTTT
    TTGAATTCTA CCCAGGACTT TCTCACCAGC CAGCTGTCCG AAGTTGTTGT TGAAGAAAGT
    AAGCAGTTCG GGAATAAGAT AATAAGCTAC TTTGAACGTT ATCTGCAGTG GGTCGAGATG
    GCCATCACCC AGCAATTTGC AGCCTGCAAA CCGGCGGCCA CTGCTCTGGA TTCGGCTGTT
    AATGTCTTTC TGTGCAGCTA CATCGTCGAC CCCCTGAATC TGTTTTGGTT TGGCGTGGGA
    AAAGCGACCA TACTTTTACT TCCTGCTGTC ATTTTTGCCG TGAAGCTGGC TAAGTACTTC
    CGTCGAATGT ATTCTGAAGA TGTGTATGAG GATGAGCCAG TGAACAAGGT TCAAAGTAAA
    CCCAGGGCTC AAACTGTACC CAGGGTTCAA ACTGTACCCA TGGTTCAAAC TGTACCCATG
    ACAAAGGCGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804701.1
    CDS467..3061
    Translation

    Target ORF information:

    RefSeq Version XM_010806399.2
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806399.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTAAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTGCTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAA TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT CTGTGGCACG
    TTGGGCTATG ACCAGAAAGC CACCCCAACC AGACGAGGCT GCGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGACTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCAAC
    CAGCTGCCCT CAGGAAACTT GAAAACCAGC TTGAAAAGTC ACACAGATAC CTTAACAAAT
    ATCCATGAGA ACCAGGTCGT TCCTCTGCAG AATTCAATGA ACGCCATGCA CCAGCAAATG
    AAGGGGCTTC AGTACAGGAC CAGTGGACTG AAGGTCAGAG TGTCCACCAC CATTTTCTTT
    TTGAATTCTA CCCAGGACTT TCTCACCAGC CAGCTGTCCG AAGTTGTTGT TGAAGAAAGT
    AAGCAGTTCG GGAATAAGAT AATAAGCTAC TTTGAACGTT ATCTGCAGTG GGTCGAGATG
    GCCATCACCC AGCAATTTGC AGCCTGCAAA CCGGCGGCCA CTGCTCTGGA TTCGGCTGTT
    AATGTCTTTC TGTGCAGCTA CATCGTCGAC CCCCTGAATC TGTTTTGGTT TGGCGTGGGA
    AAAGCGACCA TACTTTTACT TCCTGCTGTC ATTTTTGCCG TGAAGCTGGC TAAGTACTTC
    CGTCGAATGT ATTCTGAAGA TGTGTATGAG GATGAGCCAG TGAACAAGGT TCAAAGTAAA
    CCCAGGGCTC AAACTGTACC CAGGGTTCAA ACTGTACCCA TGGTTCAAAC TGTACCCATG
    ACAAAGGCGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38064
    Clone ID Related Accession (Same CDS sequence) NM_001245952.2
    Accession Version NM_001245952.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product prominin-1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HQ159409.1. On Aug 30, 2012 this sequence version replaced NM_001245952.1. ##Evidence-Data-START## Transcript exon combination :: HQ159409.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTAAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTGCTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAA TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT GTGTGGCACG
    TTGGGCTATG ACCAGAAAGC TACCCCAACC AGACGAGGCT GTGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGATTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCAAC
    CAGCTGCCCT CAGGAAACTT GAAAACCAGC TTGAAAAGTC ACACAGATAC CTTAACAAAT
    ATCCAGGAGA ACCAGGTCGT TCCTCTGCAG AATTCAATGA ACGCCATGCA CCAGCAAATG
    AAGGGGCTTC AGTACAGGAC CAGTGGACTG AAGGTCAGAG TGTCCACCAC CATTTTCTTT
    TTGAATTCTA CCCAGGACTT TCTCACCAGC CAGCTGTCCG AAGTTGTTGT TGAAGAAAGT
    AAGCAGTTCG GGAATAAGAT AATAAGCTAC TTTGAACGTT ATCTGCAGTG GGTCGAGATG
    GCCATCACCC AGCAATTTGC AGCCTGCAGA CCGGTGGCCA CTGCTCTGGA TTCAGCTGTT
    AACGTCTTTC TGTGCAGCTA CATCGTCGAC CCCCTGAATC TGTTTTGGTT TGGCGTGGGA
    AAAGCGACCA TACTTTTACT TCCTGCTGTC ATTTTTGCCG TGAAGCTGGC TAAGTACTTC
    CGTCGAATGT ATTCTGAAGA TGTGTATGAG GATGAGCCAG TGAACAAGGT TCAAAGTAAA
    CCCAGGGCTC AAACTGTACC CAGGGTTCAA ACTGTACCCA TGGTTCAAAC TGTACCCATG
    ACAAATATGA GACATGCTAA TACCCGTTCT CGTGTAGAAC AAGGCACACA GACAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001232881.1
    CDS91..2730
    Translation

    Target ORF information:

    RefSeq Version NM_001245952.2
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001245952.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTAAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTGCTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAA TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT GTGTGGCACG
    TTGGGCTATG ACCAGAAAGC TACCCCAACC AGACGAGGCT GTGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGATTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCAAC
    CAGCTGCCCT CAGGAAACTT GAAAACCAGC TTGAAAAGTC ACACAGATAC CTTAACAAAT
    ATCCAGGAGA ACCAGGTCGT TCCTCTGCAG AATTCAATGA ACGCCATGCA CCAGCAAATG
    AAGGGGCTTC AGTACAGGAC CAGTGGACTG AAGGTCAGAG TGTCCACCAC CATTTTCTTT
    TTGAATTCTA CCCAGGACTT TCTCACCAGC CAGCTGTCCG AAGTTGTTGT TGAAGAAAGT
    AAGCAGTTCG GGAATAAGAT AATAAGCTAC TTTGAACGTT ATCTGCAGTG GGTCGAGATG
    GCCATCACCC AGCAATTTGC AGCCTGCAGA CCGGTGGCCA CTGCTCTGGA TTCAGCTGTT
    AACGTCTTTC TGTGCAGCTA CATCGTCGAC CCCCTGAATC TGTTTTGGTT TGGCGTGGGA
    AAAGCGACCA TACTTTTACT TCCTGCTGTC ATTTTTGCCG TGAAGCTGGC TAAGTACTTC
    CGTCGAATGT ATTCTGAAGA TGTGTATGAG GATGAGCCAG TGAACAAGGT TCAAAGTAAA
    CCCAGGGCTC AAACTGTACC CAGGGTTCAA ACTGTACCCA TGGTTCAAAC TGTACCCATG
    ACAAATATGA GACATGCTAA TACCCGTTCT CGTGTAGAAC AAGGCACACA GACAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20695
    Clone ID Related Accession (Same CDS sequence) XM_005208330.3
    Accession Version XM_005208330.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product prominin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103992.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTAAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTGCTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAA TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT CTGTGGCACG
    TTGGGCTATG ACCAGAAAGC CACCCCAACC AGACGAGGCT GCGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGACTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCAAC
    CAGCTGCCCT CAGGAAACTT GAAAACCAGC TTGAAAAGTC ACACAGATAC CTTAACAAAT
    ATCCATGAGA ACCAGGTCGT TCCTCTGCAG AATTCAATGA ACGCCATGCA CCAGCAAATG
    AAGGGGCTTC AGTACAGGAC CAGTGGACTG AAGGTCAGAG TGTCCACCAC CATTTTCTTT
    TTGAATTCTA CCCAGGACTT TCTCACCAGC CAGCTGTCCG AAGTTGTTGT TGAAGAAAGT
    AAGCAGTTCG GGAATAAGAT AATAAGCTAC TTTGAACGTT ATCTGCAGTG GGTCGAGATG
    GCCATCACCC AGCAATTTGC AGCCTGCAAA CCGGCGGCCA CTGCTCTGGA TTCGGCTGTT
    AATGTCTTTC TGTGCAGCTA CATCGTCGAC CCCCTGAATC TGTTTTGGTT TGGCGTGGGA
    AAAGCGACCA TACTTTTACT TCCTGCTGTC ATTTTTGCCG TGAAGCTGGC TAAGTACTTC
    CGTCGAATGT ATTCTGAAGA TGTGTATGAG GATGAGCCAG TGAACAAGGT TCAAAGTAAA
    CCCAGGGCTC AAACTGTACC CAGGGTTCAA ACTGTACCCA TGGTTCAAAC TGTACCCATG
    ACAAATATGA GACATGCTAA TACCCGTTCT CGTGTAGAAC AAGGCACACA GACAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208387.1
    CDS284..2923
    Translation

    Target ORF information:

    RefSeq Version XM_005208330.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208330.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTAAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTGCTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAA TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT CTGTGGCACG
    TTGGGCTATG ACCAGAAAGC CACCCCAACC AGACGAGGCT GCGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGACTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCAAC
    CAGCTGCCCT CAGGAAACTT GAAAACCAGC TTGAAAAGTC ACACAGATAC CTTAACAAAT
    ATCCATGAGA ACCAGGTCGT TCCTCTGCAG AATTCAATGA ACGCCATGCA CCAGCAAATG
    AAGGGGCTTC AGTACAGGAC CAGTGGACTG AAGGTCAGAG TGTCCACCAC CATTTTCTTT
    TTGAATTCTA CCCAGGACTT TCTCACCAGC CAGCTGTCCG AAGTTGTTGT TGAAGAAAGT
    AAGCAGTTCG GGAATAAGAT AATAAGCTAC TTTGAACGTT ATCTGCAGTG GGTCGAGATG
    GCCATCACCC AGCAATTTGC AGCCTGCAAA CCGGCGGCCA CTGCTCTGGA TTCGGCTGTT
    AATGTCTTTC TGTGCAGCTA CATCGTCGAC CCCCTGAATC TGTTTTGGTT TGGCGTGGGA
    AAAGCGACCA TACTTTTACT TCCTGCTGTC ATTTTTGCCG TGAAGCTGGC TAAGTACTTC
    CGTCGAATGT ATTCTGAAGA TGTGTATGAG GATGAGCCAG TGAACAAGGT TCAAAGTAAA
    CCCAGGGCTC AAACTGTACC CAGGGTTCAA ACTGTACCCA TGGTTCAAAC TGTACCCATG
    ACAAATATGA GACATGCTAA TACCCGTTCT CGTGTAGAAC AAGGCACACA GACAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20699
    Clone ID Related Accession (Same CDS sequence) XM_005208337.3
    Accession Version XM_005208337.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product prominin-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208337.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGCTG CCGAGCCCCG TCCCGCTGGC CTGAAATGGG CACTTATGGA GCGTCTGCTC 
    GTGGCAGGTA CCCTCCTTGG CGCCTTATCG CTAACTGTAC ATGCTCTACG ATGTTCACGA
    CGACCTTACA AGATTATCTA CTATGAGATC GGGATTATTA TCTGTGCTGC CCTGGGATTG
    CTGTTTGTTA TTCTGATGCC TCTGGTGGGC TTTTTCTTTT GTCTGTGTCG TTGCTGTAAC
    AAGTGTGGTG GAGAAATGCA CCAGCGACAG AAGAAAAGTG GTCCCTTCCT CAAGAAATAC
    TTTACAATCT CCCTCCTGGT GATCTGTGTG TTCATAAGCA TCGGGATCAT CTATGGTTTT
    GCCGCAAATC ATTACATGCG GACTAACGTG GAAGAGACTC GGAAATTGTC AGAAAGTAAC
    TTAAACGACC TGAGAACTCT CCTGAATGTG GTGCCAGGGC AAATTGACTA CATACTGGAC
    CAGTTTACCC TGCCTAAGGA GAAAGCATTT GACGATCTGG ACAATATTAA CTTGTTGGTG
    GGAGGCAGCA TTTACGAGCG TCTCAAACCC AAAGTGCTCC CTGTGCTGAA GGACATCAAG
    GACTTGGCAG AAGACATGAA AACTAATAGA GATACCCTGG TCCGAATGAA CACTGTCTTA
    ACGGACATGA AGCAGTCATC TGCACAACTT CGCACCAGCC TGAGAGACGT GAAAACGAAC
    ATGGAGCAAA CACTCATGGA TCCCCAGTGC TCCTCACCTG CAGCGGCGCC CACGTGCGAC
    AGCATCAGGA AGTCTCTGAG CGTCCTGGAT GGCAGTGCCA ATTTTGATCA CCTCCCGTCT
    TTGGATGGAC ACATTACTCA GCTTGATGGA CTTCTTCAAA CAGATTTGTC CGGCCTGGTC
    CAAAAGGCCA ATGAATCTCT TTCTAATATA CCTGAGGAAG TGCAAAATCA AACCAGGGAC
    TTCATATCAG AGTTTAAAAA GACCTTGAAT TCCCTTCAAT CTGATGTCAA AAATATAAGC
    ACGAAGATTC CTATTCAGAA GACACTGTCG AATTTCGTCC GTTACATTAA TGACTCTGAA
    GATTATATCC TCCAGTATTT ACCCACTATG GAAGAATGCG ATTCATACAG GTGGCTGGTT
    TGCTTGGTCA TCTGCTGCTT GCTGACCCTC ATCTTGATTT TTTACCTCCT GGGCTTGCTC
    TGTGGCACGT TGGGCTATGA CCAGAAAGCC ACCCCAACCA GACGAGGCTG CGTCTCGAAC
    ACCGGGGGCC TCCTCCTCAT GGTTGGGGTT GGACTCAGTT TCTTCTTTTC TTGGATAATC
    ATGACCATCG TGGTGCTGAC TTTTGTCACG GGTGGAAATA TGGAAAAACT GGTCTGTGAG
    CCTTACAGAA ACAAAAAGTT ATTCCAGGTT TTGGACACTC CATATTTACT AAATGAGGAT
    TGGAAATACT ATCTCTCTGG CCTAGTATTT AACAAACCAG ATATTAATCT CACTTTTGAA
    CAAGTTTACA GTGACTGCAA AGAAAATAAA GGCCTTTATG CCACACTTAA GCTCGACCAC
    ATCTACAATG TCAGTGAACA GCTCAACATT ACAAAGCATA CTGGAGACAT AAATAGTAAT
    TTGGAAAATA TGAATATAAG AATTGAGGAT ATAGAACTGC TGGATAAAAC AGGAATGAAA
    ACCCTTATGG ATCTCAGATC TTCTGGAATA GACGATATAG ACTATGCTGC CTACTTGAAC
    GCGACCGAGA GAAGTCCCAC GAGAGTAAAT CTTATCTCAT TTGCAAATAA TCTCCGAAGA
    AAAGCCAACC AGCTGCCCTC AGGAAACTTG AAAACCAGCT TGAAAAGTCA CACAGATACC
    TTAACAAATA TCCATGAGAA CCAGGTCGTT CCTCTGCAGA ATTCAATGAA CGCCATGCAC
    CAGCAAATGA AGGGGCTTCA GTACAGGACC AGTGGACTGA AGGTCAGAGT GTCCACCACC
    ATTTTCTTTT TGAATTCTAC CCAGGACTTT CTCACCAGCC AGCTGTCCGA AGTTGTTGTT
    GAAGAAAGTA AGCAGTTCGG GAATAAGATA ATAAGCTACT TTGAACGTTA TCTGCAGTGG
    GTCGAGATGG CCATCACCCA GCAATTTGCA GCCTGCAAAC CGGCGGCCAC TGCTCTGGAT
    TCGGCTGTTA ATGTCTTTCT GTGCAGCTAC ATCGTCGACC CCCTGAATCT GTTTTGGTTT
    GGCGTGGGAA AAGCGACCAT ACTTTTACTT CCTGCTGTCA TTTTTGCCGT GAAGCTGGCT
    AAGTACTTCC GTCGAATGTA TTCTGAAGAT GTGTATGAGG ATGAGCCAGT GAACAAGGTT
    CAAAGTAAAC CCAGGGCTCA AACTGTACCC AGGGTTCAAA CTGTACCCAT GGTTCAAACT
    GTACCCATGA CAAATATGAG ACATGCTAAT ACCCGTTCTC GTGTAGAACA AGGCACACAG
    ACAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208394.1
    CDS620..3088
    Translation

    Target ORF information:

    RefSeq Version XM_005208337.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208337.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGAAGGCTG CCGAGCCCCG TCCCGCTGGC CTGAAATGGG CACTTATGGA GCGTCTGCTC 
    GTGGCAGGTA CCCTCCTTGG CGCCTTATCG CTAACTGTAC ATGCTCTACG ATGTTCACGA
    CGACCTTACA AGATTATCTA CTATGAGATC GGGATTATTA TCTGTGCTGC CCTGGGATTG
    CTGTTTGTTA TTCTGATGCC TCTGGTGGGC TTTTTCTTTT GTCTGTGTCG TTGCTGTAAC
    AAGTGTGGTG GAGAAATGCA CCAGCGACAG AAGAAAAGTG GTCCCTTCCT CAAGAAATAC
    TTTACAATCT CCCTCCTGGT GATCTGTGTG TTCATAAGCA TCGGGATCAT CTATGGTTTT
    GCCGCAAATC ATTACATGCG GACTAACGTG GAAGAGACTC GGAAATTGTC AGAAAGTAAC
    TTAAACGACC TGAGAACTCT CCTGAATGTG GTGCCAGGGC AAATTGACTA CATACTGGAC
    CAGTTTACCC TGCCTAAGGA GAAAGCATTT GACGATCTGG ACAATATTAA CTTGTTGGTG
    GGAGGCAGCA TTTACGAGCG TCTCAAACCC AAAGTGCTCC CTGTGCTGAA GGACATCAAG
    GACTTGGCAG AAGACATGAA AACTAATAGA GATACCCTGG TCCGAATGAA CACTGTCTTA
    ACGGACATGA AGCAGTCATC TGCACAACTT CGCACCAGCC TGAGAGACGT GAAAACGAAC
    ATGGAGCAAA CACTCATGGA TCCCCAGTGC TCCTCACCTG CAGCGGCGCC CACGTGCGAC
    AGCATCAGGA AGTCTCTGAG CGTCCTGGAT GGCAGTGCCA ATTTTGATCA CCTCCCGTCT
    TTGGATGGAC ACATTACTCA GCTTGATGGA CTTCTTCAAA CAGATTTGTC CGGCCTGGTC
    CAAAAGGCCA ATGAATCTCT TTCTAATATA CCTGAGGAAG TGCAAAATCA AACCAGGGAC
    TTCATATCAG AGTTTAAAAA GACCTTGAAT TCCCTTCAAT CTGATGTCAA AAATATAAGC
    ACGAAGATTC CTATTCAGAA GACACTGTCG AATTTCGTCC GTTACATTAA TGACTCTGAA
    GATTATATCC TCCAGTATTT ACCCACTATG GAAGAATGCG ATTCATACAG GTGGCTGGTT
    TGCTTGGTCA TCTGCTGCTT GCTGACCCTC ATCTTGATTT TTTACCTCCT GGGCTTGCTC
    TGTGGCACGT TGGGCTATGA CCAGAAAGCC ACCCCAACCA GACGAGGCTG CGTCTCGAAC
    ACCGGGGGCC TCCTCCTCAT GGTTGGGGTT GGACTCAGTT TCTTCTTTTC TTGGATAATC
    ATGACCATCG TGGTGCTGAC TTTTGTCACG GGTGGAAATA TGGAAAAACT GGTCTGTGAG
    CCTTACAGAA ACAAAAAGTT ATTCCAGGTT TTGGACACTC CATATTTACT AAATGAGGAT
    TGGAAATACT ATCTCTCTGG CCTAGTATTT AACAAACCAG ATATTAATCT CACTTTTGAA
    CAAGTTTACA GTGACTGCAA AGAAAATAAA GGCCTTTATG CCACACTTAA GCTCGACCAC
    ATCTACAATG TCAGTGAACA GCTCAACATT ACAAAGCATA CTGGAGACAT AAATAGTAAT
    TTGGAAAATA TGAATATAAG AATTGAGGAT ATAGAACTGC TGGATAAAAC AGGAATGAAA
    ACCCTTATGG ATCTCAGATC TTCTGGAATA GACGATATAG ACTATGCTGC CTACTTGAAC
    GCGACCGAGA GAAGTCCCAC GAGAGTAAAT CTTATCTCAT TTGCAAATAA TCTCCGAAGA
    AAAGCCAACC AGCTGCCCTC AGGAAACTTG AAAACCAGCT TGAAAAGTCA CACAGATACC
    TTAACAAATA TCCATGAGAA CCAGGTCGTT CCTCTGCAGA ATTCAATGAA CGCCATGCAC
    CAGCAAATGA AGGGGCTTCA GTACAGGACC AGTGGACTGA AGGTCAGAGT GTCCACCACC
    ATTTTCTTTT TGAATTCTAC CCAGGACTTT CTCACCAGCC AGCTGTCCGA AGTTGTTGTT
    GAAGAAAGTA AGCAGTTCGG GAATAAGATA ATAAGCTACT TTGAACGTTA TCTGCAGTGG
    GTCGAGATGG CCATCACCCA GCAATTTGCA GCCTGCAAAC CGGCGGCCAC TGCTCTGGAT
    TCGGCTGTTA ATGTCTTTCT GTGCAGCTAC ATCGTCGACC CCCTGAATCT GTTTTGGTTT
    GGCGTGGGAA AAGCGACCAT ACTTTTACTT CCTGCTGTCA TTTTTGCCGT GAAGCTGGCT
    AAGTACTTCC GTCGAATGTA TTCTGAAGAT GTGTATGAGG ATGAGCCAGT GAACAAGGTT
    CAAAGTAAAC CCAGGGCTCA AACTGTACCC AGGGTTCAAA CTGTACCCAT GGTTCAAACT
    GTACCCATGA CAAATATGAG ACATGCTAAT ACCCGTTCTC GTGTAGAACA AGGCACACAG
    ACAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235444
    Clone ID Related Accession (Same CDS sequence) XM_024993438.1
    Accession Version XM_024993438.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product prominin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTCAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTACTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAG TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT GTGTGGCACG
    TTGGGCTATG ACCAGAAAGC TACCCCAACC AGACGAGGCT GTGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGACTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCCAA
    CCACCCTCAG GAAACTTGAA AACCAGCTTG AAAAGTCACA CAGATACCTT AACAAATATC
    CAGGAGAACC AGGTCGTTCC TCTGCAGAAT TCAATGAACG CCATGCACCA GCAAATGAAG
    GGGCTTCAGT ACAGGACCAG TGGACTGAAG GTCAGAGTGT CCACCACCAT TTTCTTTTTG
    AATTCTACCC AGGACTTTCT CACCAGCCAG CTGTCCGAAG TTGTTGTTGA AGAAAGTAAG
    CAGTTCGGGA ATAAGATAAT AAGCTACTTT GAACGTTATC TGCAGTGGGT CGAGATGGCC
    ATCACCCAGC AATTTGCAGC CTGCAGACCG GTGGCCACTG CTCTGGATTC AGCTGTTAAC
    GTCTTTCTGT GCAGCTACAT CGTCGACCCC CTGAATCTGT TTTGGTTTGG CGTGGGAAAA
    GCGACCATAC TTTTACTTCC TGCTGTCATT TTTGCCGTGA AGCTGGCTAA GTACTTCCGT
    CGAATGTATT CTGAAGATGT GTATGAGGAT GAGCCAGTGA ACAAGGTTCA AAGTAAACCC
    AGGGCTCAAA CTGTACCCAG GGTTCAAACT GTACCCATGG TTCAAACTGT ACCCATGACA
    AATATGAGAC ATGCTAATAC CCGTTCTCGT GTAGAACAAG GCACACAGAC AATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849206.1
    CDS514..3150
    Translation

    Target ORF information:

    RefSeq Version XM_024993438.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGCCCTGC TCCTTGGCTT CCTCCTGCTG CTGGGACTCT GTGAGGACAC CCTGTCAGAG 
    GAGCTGTCAT CATCCGGGTA CCGTCCTGAT GGTTTGGAAT TCCAGTTGCC TCCAACCTCC
    TATCAGACCA GCGACTCTTA TGATCCTGGT CTCGCTGGCT TCTTCTTTCA GATTGTACGC
    TTCTTTGTCC AAATTGTGCA GCCCAATGCT TTCCCTGAAG ATATTTTAAG AAAGATTATA
    CAGAAGAAGT TTGACCTCTC AAAGGAGTAT GATAAGCCAG AAAACGTAGT CTTGACTCTG
    AAGATTATCT ACTATGAGAT CGGGATTATT ATCTGTGCTG CCCTGGGATT GCTGTTTGTT
    ATTCTGATGC CTCTGGTGGG CTTTTTCTTT TGTCTGTGTC GTTGCTGTAA CAAGTGTGGT
    GGAGAAATGC ACCAGCGACA GAAGAAAAGT GGTCCCTTCC TCAAGAAATA CTTTACAATC
    TCCCTCCTGG TGATCTGTGT GTTCATAAGC ATCGGGATCA TCTATGGTTT TGCCGCAAAT
    CATTACATGC GGACTAACGT GGAAGAGACT CGGAAATTGT CAGAAAGTAA CTTCAACGAC
    CTGAGAACTC TCCTGAATGT GGTGCCAGGG CAAATTGACT ACATACTGGA CCAGTTTACC
    CTGCCTAAGG AGAAAGCATT TGACGATCTG GACAATATTA ACTTGTTGGT GGGAGGCAGC
    ATTTACGAGC GTCTCAAACC CAAAGTACTC CCTGTGCTGA AGGACATCAA GGACTTGGCA
    GAAGACATGA AAACTAATAG AGATACCCTG GTCCGAATGA ACACTGTCTT AACGGACATG
    AAGCAGTCAT CTGCACAACT TCGCACCAGC CTGAGAGACG TGAAAACGAA CATGGAGCAA
    ACACTCATGG ATCCCCAGTG CTCCTCACCT GCAGCGGCGC CCACGTGCGA CAGCATCAGG
    AAGTCTCTGA GCGTCCTGGA TGGCAGTGCC AATTTTGATC ACCTCCCGTC TTTGGATGGA
    CACATTACTC AGCTTGATGG ACTTCTTCAA ACAGATTTGT CCGGCCTGGT CCAAAAGGCC
    AATGAATCTC TTTCTAATAT ACCTGAGGAA GTGCAAAATC AAACCAGGGA CTTCATATCA
    GAGTTTAAAA AGACCTTGAA TTCCCTTCAG TCTGATGTCA AAAATATAAG CACGAAGATT
    CCTATTCAGA AGACACTGTC GAATTTCGTC CGTTACATTA ATGACTCTGA AGATTATATC
    CTCCAGTATT TACCCACTAT GGAAGAATGC GATTCATACA GGTGGCTGGT TTGCTTGGTC
    ATCTGCTGCT TGCTGACCCT CATCTTGATT TTTTACCTCC TGGGCTTGCT GTGTGGCACG
    TTGGGCTATG ACCAGAAAGC TACCCCAACC AGACGAGGCT GTGTCTCGAA CACCGGGGGC
    CTCCTCCTCA TGGTTGGGGT TGGACTCAGT TTCTTCTTTT CTTGGATAAT CATGACCATC
    GTGGTGCTGA CTTTTGTCAC GGGTGGAAAT ATGGAAAAAC TGGTCTGTGA GCCTTACAGA
    AACAAAAAGT TATTCCAGGT TTTGGACACT CCATATTTAC TAAATGAGGA TTGGAAATAC
    TATCTCTCTG GCCTAGTATT TAACAAACCA GATATTAATC TCACTTTTGA ACAAGTTTAC
    AGTGACTGCA AAGAAAATAA AGGCCTTTAT GCCACACTTA AGCTCGACCA CATCTACAAT
    GTCAGTGAAC AGCTCAACAT TACAAAGCAT ACTGGAGACA TAAATAGTAA TTTGGAAAAT
    ATGAATATAA GAATTGAGGA TATAGAACTG CTGGATAAAA CAGGAATGAA AACCCTTATG
    GATCTCAGAT CTTCTGGAAT AGACGATATA GACTATGCTG CCTACTTGAA CGCGACCGAG
    AGAAGTCCCA CGAGAGTAAA TCTTATCTCA TTTGCAAATA ATCTCCGAAG AAAAGCCCAA
    CCACCCTCAG GAAACTTGAA AACCAGCTTG AAAAGTCACA CAGATACCTT AACAAATATC
    CAGGAGAACC AGGTCGTTCC TCTGCAGAAT TCAATGAACG CCATGCACCA GCAAATGAAG
    GGGCTTCAGT ACAGGACCAG TGGACTGAAG GTCAGAGTGT CCACCACCAT TTTCTTTTTG
    AATTCTACCC AGGACTTTCT CACCAGCCAG CTGTCCGAAG TTGTTGTTGA AGAAAGTAAG
    CAGTTCGGGA ATAAGATAAT AAGCTACTTT GAACGTTATC TGCAGTGGGT CGAGATGGCC
    ATCACCCAGC AATTTGCAGC CTGCAGACCG GTGGCCACTG CTCTGGATTC AGCTGTTAAC
    GTCTTTCTGT GCAGCTACAT CGTCGACCCC CTGAATCTGT TTTGGTTTGG CGTGGGAAAA
    GCGACCATAC TTTTACTTCC TGCTGTCATT TTTGCCGTGA AGCTGGCTAA GTACTTCCGT
    CGAATGTATT CTGAAGATGT GTATGAGGAT GAGCCAGTGA ACAAGGTTCA AAGTAAACCC
    AGGGCTCAAA CTGTACCCAG GGTTCAAACT GTACCCATGG TTCAAACTGT ACCCATGACA
    AATATGAGAC ATGCTAATAC CCGTTCTCGT GTAGAACAAG GCACACAGAC AATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90973
    Clone ID Related Accession (Same CDS sequence) XM_010806402.2
    Accession Version XM_010806402.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product prominin-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806402.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATCCTGG TCTCGCTGGC TTCTTCTTTC AGATTGTACG CTTCTTTGTC CAAATTGTGC 
    AGCCCAATGC TTTCCCTGAA GATTATCTAC TATGAGATCG GGATTATTAT CTGTGCTGCC
    CTGGGATTGC TGTTTGTTAT TCTGATGCCT CTGGTGGGCT TTTTCTTTTG TCTGTGTCGT
    TGCTGTAACA AGTGTGGTGG AGAAATGCAC CAGCGACAGA AGAAAAGTGG TCCCTTCCTC
    AAGAAATACT TTACAATCTC CCTCCTGGTG ATCTGTGTGT TCATAAGCAT CGGGATCATC
    TATGGTTTTG CCGCAAATCA TTACATGCGG ACTAACGTGG AAGAGACTCG GAAATTGTCA
    GAAAGTAACT TAAACGACCT GAGAACTCTC CTGAATGTGG TGCCAGGGCA AATTGACTAC
    ATACTGGACC AGTTTACCCT GCCTAAGGAG AAAGCATTTG ACGATCTGGA CAATATTAAC
    TTGTTGGTGG GAGGCAGCAT TTACGAGCGT CTCAAACCCA AAGTGCTCCC TGTGCTGAAG
    GACATCAAGG ACTTGGCAGA AGACATGAAA ACTAATAGAG ATACCCTGGT CCGAATGAAC
    ACTGTCTTAA CGGACATGAA GCAGTCATCT GCACAACTTC GCACCAGCCT GAGAGACGTG
    AAAACGAACA TGGAGCAAAC ACTCATGGAT CCCCAGTGCT CCTCACCTGC AGCGGCGCCC
    ACGTGCGACA GCATCAGGAA GTCTCTGAGC GTCCTGGATG GCAGTGCCAA TTTTGATCAC
    CTCCCGTCTT TGGATGGACA CATTACTCAG CTTGATGGAC TTCTTCAAAC AGATTTGTCC
    GGCCTGGTCC AAAAGGCCAA TGAATCTCTT TCTAATATAC CTGAGGAAGT GCAAAATCAA
    ACCAGGGACT TCATATCAGA GTTTAAAAAG ACCTTGAATT CCCTTCAATC TGATGTCAAA
    AATATAAGCA CGAAGATTCC TATTCAGAAG ACACTGTCGA ATTTCGTCCG TTACATTAAT
    GACTCTGAAG ATTATATCCT CCAGTATTTA CCCACTATGG AAGAATGCGA TTCATACAGG
    TGGCTGGTTT GCTTGGTCAT CTGCTGCTTG CTGACCCTCA TCTTGATTTT TTACCTCCTG
    GGCTTGCTCT GTGGCACGTT GGGCTATGAC CAGAAAGCCA CCCCAACCAG ACGAGGCTGC
    GTCTCGAACA CCGGGGGCCT CCTCCTCATG GTTGGGGTTG GACTCAGTTT CTTCTTTTCT
    TGGATAATCA TGACCATCGT GGTGCTGACT TTTGTCACGG GTGGAAATAT GGAAAAACTG
    GTCTGTGAGC CTTACAGAAA CAAAAAGTTA TTCCAGGTTT TGGACACTCC ATATTTACTA
    AATGAGGATT GGAAATACTA TCTCTCTGGC CTAGTATTTA ACAAACCAGA TATTAATCTC
    ACTTTTGAAC AAGTTTACAG TGACTGCAAA GAAAATAAAG GCCTTTATGC CACACTTAAG
    CTCGACCACA TCTACAATGT CAGTGAACAG CTCAACATTA CAAAGCATAC TGGAGACATA
    AATAGTAATT TGGAAAATAT GAATATAAGA ATTGAGGATA TAGAACTGCT GGATAAAACA
    GGAATGAAAA CCCTTATGGA TCTCAGATCT TCTGGAATAG ACGATATAGA CTATGCTGCC
    TACTTGAACG CGACCGAGAG AAGTCCCACG AGAGTAAATC TTATCTCATT TGCAAATAAT
    CTCCGAAGAA AAGCCAACCA GCTGCCCTCA GGAAACTTGA AAACCAGCTT GAAAAGTCAC
    ACAGATACCT TAACAAATAT CCATGAGAAC CAGGTCGTTC CTCTGCAGAA TTCAATGAAC
    GCCATGCACC AGCAAATGAA GGGGCTTCAG TACAGGACCA GTGGACTGAA GGTCAGAGTG
    TCCACCACCA TTTTCTTTTT GAATTCTACC CAGGACTTTC TCACCAGCCA GCTGTCCGAA
    GTTGTTGTTG AAGAAAGTAA GCAGTTCGGG AATAAGATAA TAAGCTACTT TGAACGTTAT
    CTGCAGTGGG TCGAGATGGC CATCACCCAG CAATTTGCAG CCTGCAAACC GGCGGCCACT
    GCTCTGGATT CGGCTGTTAA TGTCTTTCTG TGCAGCTACA TCGTCGACCC CCTGAATCTG
    TTTTGGTTTG GCGTGGGAAA AGCGACCATA CTTTTACTTC CTGCTGTCAT TTTTGCCGTG
    AAGCTGGCTA AGTACTTCCG TCGAATGTAT TCTGAAGATG TGTATGAGGA TGAGCCAGTG
    AACAAGGTTC AAAGTAAACC CAGGGCTCAA ACTGTACCCA GGGTTCAAAC TGTACCCATG
    GTTCAAACTG TACCCATGAC AAATATGAGA CATGCTAATA CCCGTTCTCG TGTAGAACAA
    GGCACACAGA CAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804704.2
    CDS14..2431
    Translation

    Target ORF information:

    RefSeq Version XM_010806402.2
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806402.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATCCTGG TCTCGCTGGC TTCTTCTTTC AGATTGTACG CTTCTTTGTC CAAATTGTGC 
    AGCCCAATGC TTTCCCTGAA GATTATCTAC TATGAGATCG GGATTATTAT CTGTGCTGCC
    CTGGGATTGC TGTTTGTTAT TCTGATGCCT CTGGTGGGCT TTTTCTTTTG TCTGTGTCGT
    TGCTGTAACA AGTGTGGTGG AGAAATGCAC CAGCGACAGA AGAAAAGTGG TCCCTTCCTC
    AAGAAATACT TTACAATCTC CCTCCTGGTG ATCTGTGTGT TCATAAGCAT CGGGATCATC
    TATGGTTTTG CCGCAAATCA TTACATGCGG ACTAACGTGG AAGAGACTCG GAAATTGTCA
    GAAAGTAACT TAAACGACCT GAGAACTCTC CTGAATGTGG TGCCAGGGCA AATTGACTAC
    ATACTGGACC AGTTTACCCT GCCTAAGGAG AAAGCATTTG ACGATCTGGA CAATATTAAC
    TTGTTGGTGG GAGGCAGCAT TTACGAGCGT CTCAAACCCA AAGTGCTCCC TGTGCTGAAG
    GACATCAAGG ACTTGGCAGA AGACATGAAA ACTAATAGAG ATACCCTGGT CCGAATGAAC
    ACTGTCTTAA CGGACATGAA GCAGTCATCT GCACAACTTC GCACCAGCCT GAGAGACGTG
    AAAACGAACA TGGAGCAAAC ACTCATGGAT CCCCAGTGCT CCTCACCTGC AGCGGCGCCC
    ACGTGCGACA GCATCAGGAA GTCTCTGAGC GTCCTGGATG GCAGTGCCAA TTTTGATCAC
    CTCCCGTCTT TGGATGGACA CATTACTCAG CTTGATGGAC TTCTTCAAAC AGATTTGTCC
    GGCCTGGTCC AAAAGGCCAA TGAATCTCTT TCTAATATAC CTGAGGAAGT GCAAAATCAA
    ACCAGGGACT TCATATCAGA GTTTAAAAAG ACCTTGAATT CCCTTCAATC TGATGTCAAA
    AATATAAGCA CGAAGATTCC TATTCAGAAG ACACTGTCGA ATTTCGTCCG TTACATTAAT
    GACTCTGAAG ATTATATCCT CCAGTATTTA CCCACTATGG AAGAATGCGA TTCATACAGG
    TGGCTGGTTT GCTTGGTCAT CTGCTGCTTG CTGACCCTCA TCTTGATTTT TTACCTCCTG
    GGCTTGCTCT GTGGCACGTT GGGCTATGAC CAGAAAGCCA CCCCAACCAG ACGAGGCTGC
    GTCTCGAACA CCGGGGGCCT CCTCCTCATG GTTGGGGTTG GACTCAGTTT CTTCTTTTCT
    TGGATAATCA TGACCATCGT GGTGCTGACT TTTGTCACGG GTGGAAATAT GGAAAAACTG
    GTCTGTGAGC CTTACAGAAA CAAAAAGTTA TTCCAGGTTT TGGACACTCC ATATTTACTA
    AATGAGGATT GGAAATACTA TCTCTCTGGC CTAGTATTTA ACAAACCAGA TATTAATCTC
    ACTTTTGAAC AAGTTTACAG TGACTGCAAA GAAAATAAAG GCCTTTATGC CACACTTAAG
    CTCGACCACA TCTACAATGT CAGTGAACAG CTCAACATTA CAAAGCATAC TGGAGACATA
    AATAGTAATT TGGAAAATAT GAATATAAGA ATTGAGGATA TAGAACTGCT GGATAAAACA
    GGAATGAAAA CCCTTATGGA TCTCAGATCT TCTGGAATAG ACGATATAGA CTATGCTGCC
    TACTTGAACG CGACCGAGAG AAGTCCCACG AGAGTAAATC TTATCTCATT TGCAAATAAT
    CTCCGAAGAA AAGCCAACCA GCTGCCCTCA GGAAACTTGA AAACCAGCTT GAAAAGTCAC
    ACAGATACCT TAACAAATAT CCATGAGAAC CAGGTCGTTC CTCTGCAGAA TTCAATGAAC
    GCCATGCACC AGCAAATGAA GGGGCTTCAG TACAGGACCA GTGGACTGAA GGTCAGAGTG
    TCCACCACCA TTTTCTTTTT GAATTCTACC CAGGACTTTC TCACCAGCCA GCTGTCCGAA
    GTTGTTGTTG AAGAAAGTAA GCAGTTCGGG AATAAGATAA TAAGCTACTT TGAACGTTAT
    CTGCAGTGGG TCGAGATGGC CATCACCCAG CAATTTGCAG CCTGCAAACC GGCGGCCACT
    GCTCTGGATT CGGCTGTTAA TGTCTTTCTG TGCAGCTACA TCGTCGACCC CCTGAATCTG
    TTTTGGTTTG GCGTGGGAAA AGCGACCATA CTTTTACTTC CTGCTGTCAT TTTTGCCGTG
    AAGCTGGCTA AGTACTTCCG TCGAATGTAT TCTGAAGATG TGTATGAGGA TGAGCCAGTG
    AACAAGGTTC AAAGTAAACC CAGGGCTCAA ACTGTACCCA GGGTTCAAAC TGTACCCATG
    GTTCAAACTG TACCCATGAC AAATATGAGA CATGCTAATA CCCGTTCTCG TGTAGAACAA
    GGCACACAGA CAATTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90974
    Clone ID Related Accession (Same CDS sequence) XM_015471778.1
    Accession Version XM_015471778.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product prominin-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103992.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCCTCTGG TGGGCTTTTT CTTTTGTCTG TGTCGTTGCT GTAACAAGTG TGGTGGAGAA 
    ATGCACCAGC GACAGAAGAA AAGTGGTCCC TTCCTCAAGA AATACTTTAC AATCTCCCTC
    CTGGTGATCT GTGTGTTCAT AAGCATCGGG ATCATCTATG GTTTTGCCGC AAATCATTAC
    ATGCGGACTA ACGTGGAAGA GACTCGGAAA TTGTCAGAAA GTAACTTAAA CGACCTGAGA
    ACTCTCCTGA ATGTGGTGCC AGGGCAAATT GACTACATAC TGGACCAGTT TACCCTGCCT
    AAGGAGAAAG CATTTGACGA TCTGGACAAT ATTAACTTGT TGGTGGGAGG CAGCATTTAC
    GAGCGTCTCA AACCCAAAGT GCTCCCTGTG CTGAAGGACA TCAAGGACTT GGCAGAAGAC
    ATGAAAACTA ATAGAGATAC CCTGGTCCGA ATGAACACTG TCTTAACGGA CATGAAGCAG
    TCATCTGCAC AACTTCGCAC CAGCCTGAGA GACGTGAAAA CGAACATGGA GCAAACACTC
    ATGGATCCCC AGTGCTCCTC ACCTGCAGCG GCGCCCACGT GCGACAGCAT CAGGAAGTCT
    CTGAGCGTCC TGGATGGCAG TGCCAATTTT GATCACCTCC CGTCTTTGGA TGGACACATT
    ACTCAGCTTG ATGGACTTCT TCAAACAGAT TTGTCCGGCC TGGTCCAAAA GGCCAATGAA
    TCTCTTTCTA ATATACCTGA GGAAGTGCAA AATCAAACCA GGGACTTCAT ATCAGAGTTT
    AAAAAGACCT TGAATTCCCT TCAATCTGAT GTCAAAAATA TAAGCACGAA GATTCCTATT
    CAGAAGACAC TGTCGAATTT CGTCCGTTAC ATTAATGACT CTGAAGATTA TATCCTCCAG
    TATTTACCCA CTATGGAAGA ATGCGATTCA TACAGGTGGC TGGTTTGCTT GGTCATCTGC
    TGCTTGCTGA CCCTCATCTT GATTTTTTAC CTCCTGGGCT TGCTCTGTGG CACGTTGGGC
    TATGACCAGA AAGCCACCCC AACCAGACGA GGCTGCGTCT CGAACACCGG GGGCCTCCTC
    CTCATGGTTG GGGTTGGACT CAGTTTCTTC TTTTCTTGGA TAATCATGAC CATCGTGGTG
    CTGACTTTTG TCACGGGTGG AAATATGGAA AAACTGGTCT GTGAGCCTTA CAGAAACAAA
    AAGTTATTCC AGGTTTTGGA CACTCCATAT TTACTAAATG AGGATTGGAA ATACTATCTC
    TCTGGCCTAG TATTTAACAA ACCAGATATT AATCTCACTT TTGAACAAGT TTACAGTGAC
    TGCAAAGAAA ATAAAGGCCT TTATGCCACA CTTAAGCTCG ACCACATCTA CAATGTCAGT
    GAACAGCTCA ACATTACAAA GCATACTGGA GACATAAATA GTAATTTGGA AAATATGAAT
    ATAAGAATTG AGGATATAGA ACTGCTGGAT AAAACAGGAA TGAAAACCCT TATGGATCTC
    AGATCTTCTG GAATAGACGA TATAGACTAT GCTGCCTACT TGAACGCGAC CGAGAGAAGT
    CCCACGAGAG TAAATCTTAT CTCATTTGCA AATAATCTCC GAAGAAAAGC CAACCAGCTG
    CCCTCAGGAA ACTTGAAAAC CAGCTTGAAA AGTCACACAG ATACCTTAAC AAATATCCAT
    GAGAACCAGG TCGTTCCTCT GCAGAATTCA ATGAACGCCA TGCACCAGCA AATGAAGGGG
    CTTCAGTACA GGACCAGTGG ACTGAAGGTC AGAGTGTCCA CCACCATTTT CTTTTTGAAT
    TCTACCCAGG ACTTTCTCAC CAGCCAGCTG TCCGAAGTTG TTGTTGAAGA AAGTAAGCAG
    TTCGGGAATA AGATAATAAG CTACTTTGAA CGTTATCTGC AGTGGGTCGA GATGGCCATC
    ACCCAGCAAT TTGCAGCCTG CAAACCGGCG GCCACTGCTC TGGATTCGGC TGTTAATGTC
    TTTCTGTGCA GCTACATCGT CGACCCCCTG AATCTGTTTT GGTTTGGCGT GGGAAAAGCG
    ACCATACTTT TACTTCCTGC TGTCATTTTT GCCGTGAAGC TGGCTAAGTA CTTCCGTCGA
    ATGTATTCTG AAGATGTGTA TGAGGATGAG CCAGTGAACA AGGTTCAAAG TAAACCCAGG
    GCTCAAACTG TACCCAGGGT TCAAACTGTA CCCATGGTTC AAACTGTACC CATGACAAAT
    ATGAGACATG CTAATACCCG TTCTCGTGTA GAACAAGGCA CACAGACAAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327264.1
    CDS386..2659
    Translation

    Target ORF information:

    RefSeq Version XM_015471778.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prominin 1 (PROM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCCTCTGG TGGGCTTTTT CTTTTGTCTG TGTCGTTGCT GTAACAAGTG TGGTGGAGAA 
    ATGCACCAGC GACAGAAGAA AAGTGGTCCC TTCCTCAAGA AATACTTTAC AATCTCCCTC
    CTGGTGATCT GTGTGTTCAT AAGCATCGGG ATCATCTATG GTTTTGCCGC AAATCATTAC
    ATGCGGACTA ACGTGGAAGA GACTCGGAAA TTGTCAGAAA GTAACTTAAA CGACCTGAGA
    ACTCTCCTGA ATGTGGTGCC AGGGCAAATT GACTACATAC TGGACCAGTT TACCCTGCCT
    AAGGAGAAAG CATTTGACGA TCTGGACAAT ATTAACTTGT TGGTGGGAGG CAGCATTTAC
    GAGCGTCTCA AACCCAAAGT GCTCCCTGTG CTGAAGGACA TCAAGGACTT GGCAGAAGAC
    ATGAAAACTA ATAGAGATAC CCTGGTCCGA ATGAACACTG TCTTAACGGA CATGAAGCAG
    TCATCTGCAC AACTTCGCAC CAGCCTGAGA GACGTGAAAA CGAACATGGA GCAAACACTC
    ATGGATCCCC AGTGCTCCTC ACCTGCAGCG GCGCCCACGT GCGACAGCAT CAGGAAGTCT
    CTGAGCGTCC TGGATGGCAG TGCCAATTTT GATCACCTCC CGTCTTTGGA TGGACACATT
    ACTCAGCTTG ATGGACTTCT TCAAACAGAT TTGTCCGGCC TGGTCCAAAA GGCCAATGAA
    TCTCTTTCTA ATATACCTGA GGAAGTGCAA AATCAAACCA GGGACTTCAT ATCAGAGTTT
    AAAAAGACCT TGAATTCCCT TCAATCTGAT GTCAAAAATA TAAGCACGAA GATTCCTATT
    CAGAAGACAC TGTCGAATTT CGTCCGTTAC ATTAATGACT CTGAAGATTA TATCCTCCAG
    TATTTACCCA CTATGGAAGA ATGCGATTCA TACAGGTGGC TGGTTTGCTT GGTCATCTGC
    TGCTTGCTGA CCCTCATCTT GATTTTTTAC CTCCTGGGCT TGCTCTGTGG CACGTTGGGC
    TATGACCAGA AAGCCACCCC AACCAGACGA GGCTGCGTCT CGAACACCGG GGGCCTCCTC
    CTCATGGTTG GGGTTGGACT CAGTTTCTTC TTTTCTTGGA TAATCATGAC CATCGTGGTG
    CTGACTTTTG TCACGGGTGG AAATATGGAA AAACTGGTCT GTGAGCCTTA CAGAAACAAA
    AAGTTATTCC AGGTTTTGGA CACTCCATAT TTACTAAATG AGGATTGGAA ATACTATCTC
    TCTGGCCTAG TATTTAACAA ACCAGATATT AATCTCACTT TTGAACAAGT TTACAGTGAC
    TGCAAAGAAA ATAAAGGCCT TTATGCCACA CTTAAGCTCG ACCACATCTA CAATGTCAGT
    GAACAGCTCA ACATTACAAA GCATACTGGA GACATAAATA GTAATTTGGA AAATATGAAT
    ATAAGAATTG AGGATATAGA ACTGCTGGAT AAAACAGGAA TGAAAACCCT TATGGATCTC
    AGATCTTCTG GAATAGACGA TATAGACTAT GCTGCCTACT TGAACGCGAC CGAGAGAAGT
    CCCACGAGAG TAAATCTTAT CTCATTTGCA AATAATCTCC GAAGAAAAGC CAACCAGCTG
    CCCTCAGGAA ACTTGAAAAC CAGCTTGAAA AGTCACACAG ATACCTTAAC AAATATCCAT
    GAGAACCAGG TCGTTCCTCT GCAGAATTCA ATGAACGCCA TGCACCAGCA AATGAAGGGG
    CTTCAGTACA GGACCAGTGG ACTGAAGGTC AGAGTGTCCA CCACCATTTT CTTTTTGAAT
    TCTACCCAGG ACTTTCTCAC CAGCCAGCTG TCCGAAGTTG TTGTTGAAGA AAGTAAGCAG
    TTCGGGAATA AGATAATAAG CTACTTTGAA CGTTATCTGC AGTGGGTCGA GATGGCCATC
    ACCCAGCAAT TTGCAGCCTG CAAACCGGCG GCCACTGCTC TGGATTCGGC TGTTAATGTC
    TTTCTGTGCA GCTACATCGT CGACCCCCTG AATCTGTTTT GGTTTGGCGT GGGAAAAGCG
    ACCATACTTT TACTTCCTGC TGTCATTTTT GCCGTGAAGC TGGCTAAGTA CTTCCGTCGA
    ATGTATTCTG AAGATGTGTA TGAGGATGAG CCAGTGAACA AGGTTCAAAG TAAACCCAGG
    GCTCAAACTG TACCCAGGGT TCAAACTGTA CCCATGGTTC AAACTGTACC CATGACAAAT
    ATGAGACATG CTAATACCCG TTCTCGTGTA GAACAAGGCA CACAGACAAT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.