RNF220 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF220
Entrez Gene ID	616005
Full Name	ring finger protein 220
Synonyms	C1orf164,C3H1orf164
General protein information	Preferred Names ring finger protein 220 Names E3 ubiquitin-protein ligase RNF220 RING-type E3 ubiquitin transferase RNF220
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010803713.2	XP_010802015.1	E3 ubiquitin-protein ligase RNF220 isoform X2
XM_010803710.3	XP_010802012.1	E3 ubiquitin-protein ligase RNF220 isoform X1
NM_001076941.2	NP_001070409.1	E3 ubiquitin-protein ligase RNF220
XM_005204860.3	XP_005204917.1	E3 ubiquitin-protein ligase RNF220 isoform X1
XM_010803713.3	XP_010802015.1	E3 ubiquitin-protein ligase RNF220 isoform X2
XM_010803710.2	XP_010802012.1	E3 ubiquitin-protein ligase RNF220 isoform X1
XM_005204860.4	XP_005204917.1	E3 ubiquitin-protein ligase RNF220 isoform X1
XM_024989713.1	XP_024845481.1	E3 ubiquitin-protein ligase RNF220 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RNF220	XP_850815.1
Bos taurus (cattle)	RNF220	NP_001070409.1
Mus musculus (house mouse)	Rnf220	NP_080015.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003744	XP_310275.7
Homo sapiens (human)	RNF220	NP_060620.2
Rattus norvegicus (Norway rat)	Rnf220	XP_003750051.1
Gallus gallus (chicken)	RNF220	NP_001103457.1
Pan troglodytes (chimpanzee)	RNF220	XP_513101.2
Drosophila melanogaster (fruit fly)	CG4813	NP_651088.1
Xenopus tropicalis (tropical clawed frog)	rnf220.1	NP_001016196.1
Danio rerio (zebrafish)	rnf220b	XP_686354.5

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RNF220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01012	NM_001076941.2 Latest version!	Bos taurus ring finger protein 220 (RNF220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa18142	XM_005204860.3 Latest version!	Bos taurus ring finger protein 220 (RNF220), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa18142	XM_010803710.2 Latest version!	Bos taurus ring finger protein 220 (RNF220), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa55919	XM_010803713.2 Latest version!	Bos taurus ring finger protein 220 (RNF220), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa232986	XM_024989713.1 Latest version!	Bos taurus ring finger protein 220 (RNF220), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa232984	XM_010803710.3 Latest version!	Bos taurus ring finger protein 220 (RNF220), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa232984	XM_005204860.4 Latest version!	Bos taurus ring finger protein 220 (RNF220), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa232985	XM_010803713.3 Latest version!	Bos taurus ring finger protein 220 (RNF220), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF220 ( NM_001076941.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001070409.1 Bos taurus ring finger protein 220 (RNF220), mRNA.

Price & Availability↑

CloneID	OBa01012
Clone ID Related Accession (Same CDS sequence)	NM_001076941.2
Accession Version	NM_001076941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123549.1. On Aug 31, 2012 this sequence version replaced NM_001076941.1. ##Evidence-Data-START## Transcript exon combination :: BC123549.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCTGAGGAC 
GATGCCGCGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
CAGAAGCGGA TACGGGCCAC CACTCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
ATGTGCAGCG GCAAGGAGAA TCCGGACAGT GACGCTGACT TGGATGTGGA TGGGGATGAC 
ACTCTGGAAT ATGGGAAGCC ACAATACACG GAGGCCGATG TCATCCCCTG CACAGGCGAG 
GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT 
CCTAGCACAC GCATCACACC TGAGTTCTCT AAATGGGCCA GTGATGAGAT GCCATCTACC 
AGCAACGGCG AGAGCAGCAA ACAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATT 
GAGAAAATCA CCGAAGATTC AGCTGTGACT ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG 
GAACTTGAAC GGCAGCTATC TCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCA 
TACTCGATGC CCCTGACATC CATCCAGTGT TGGCATGTGC ACTGTGAGGA GTGCTGGCTA 
CGGACCCTGG GCGCCAAGAA GCTCTGCCCC CAGTGCTACA CCATCACAGC GCCTGGAGAC 
CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070409.1
CDS	372..2072
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPASKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCYTITAPGDLRRIYL

Target ORF information:

RefSeq Version	NM_001076941.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076941.2

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCTGAGGAC 
GATGCCGCGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
CAGAAGCGGA TACGGGCCAC CACTCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
ATGTGCAGCG GCAAGGAGAA TCCGGACAGT GACGCTGACT TGGATGTGGA TGGGGATGAC 
ACTCTGGAAT ATGGGAAGCC ACAATACACG GAGGCCGATG TCATCCCCTG CACAGGCGAG 
GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT 
CCTAGCACAC GCATCACACC TGAGTTCTCT AAATGGGCCA GTGATGAGAT GCCATCTACC 
AGCAACGGCG AGAGCAGCAA ACAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATT 
GAGAAAATCA CCGAAGATTC AGCTGTGACT ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG 
GAACTTGAAC GGCAGCTATC TCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCA 
TACTCGATGC CCCTGACATC CATCCAGTGT TGGCATGTGC ACTGTGAGGA GTGCTGGCTA 
CGGACCCTGG GCGCCAAGAA GCTCTGCCCC CAGTGCTACA CCATCACAGC GCCTGGAGAC 
CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_005204860.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204917.1 Bos taurus ring finger protein 220 (RNF220), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa18142
Clone ID Related Accession (Same CDS sequence)	XM_005204860.3 , XM_010803710.2
Accession Version	XM_005204860.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005204860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CAGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204917.1
CDS	372..2150
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPASKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQVCPLCSRPLAGSEQEMSRHVEHCLSKREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_005204860.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204860.3

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CAGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_010803710.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802012.1 Bos taurus ring finger protein 220 (RNF220), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa18142
Clone ID Related Accession (Same CDS sequence)	XM_005204860.3 , XM_010803710.2
Accession Version	XM_010803710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CAGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802012.1
CDS	365..2143
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPASKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQVCPLCSRPLAGSEQEMSRHVEHCLSKREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_010803710.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803710.2

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CAGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_010803713.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802015.1 Bos taurus ring finger protein 220 (RNF220), transcript variant X3, mRNA.

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CloneID	OBa55919
Clone ID Related Accession (Same CDS sequence)	XM_010803713.2
Accession Version	XM_010803713.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103874.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803713.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CAGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCTGAGGAC 
GATGCCGCGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
CAGAAGCGGA TACGGGCCAC CACTCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
ATGTGCAGCG GCAAGGAGAA TCCGGACAGT GACGCTGACT TGGATGTGGA TGGGGATGAC 
ACTCTGGAAT ATGGGAAGCC ACAATACACG GAGGCCGATG TCATCCCCTG CACAGGCGAG 
GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT 
CCTAGCACAC GCATCACACC TGAGTTCTCT AAATGGGCCA GTGATGAGAT GCCATCTACC 
AGCAACGGCG AGAGCAGCAA ACAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATT 
GAGAAAATCA CCGAAGATTC AGCTGTGACT ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG 
GAACTTGAAC GGCAGCTATC TCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCA 
TACTCGATGC CCCTGACATC CATCCAGTGT TGGCATGTGC ACTGTGAGGA GTGCTGGCTA 
CGGACCCTGG GCGCCAAGAA GCTCTGCCCC CAGTGCAACA CCATCACAGC GCCTGGAGAC 
CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802015.1
CDS	365..2065
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPASKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_010803713.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803713.2

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CAGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCTGAGGAC 
GATGCCGCGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
CAGAAGCGGA TACGGGCCAC CACTCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
ATGTGCAGCG GCAAGGAGAA TCCGGACAGT GACGCTGACT TGGATGTGGA TGGGGATGAC 
ACTCTGGAAT ATGGGAAGCC ACAATACACG GAGGCCGATG TCATCCCCTG CACAGGCGAG 
GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT 
CCTAGCACAC GCATCACACC TGAGTTCTCT AAATGGGCCA GTGATGAGAT GCCATCTACC 
AGCAACGGCG AGAGCAGCAA ACAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATT 
GAGAAAATCA CCGAAGATTC AGCTGTGACT ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG 
GAACTTGAAC GGCAGCTATC TCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCA 
TACTCGATGC CCCTGACATC CATCCAGTGT TGGCATGTGC ACTGTGAGGA GTGCTGGCTA 
CGGACCCTGG GCGCCAAGAA GCTCTGCCCC CAGTGCAACA CCATCACAGC GCCTGGAGAC 
CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_024989713.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024845481.1 Bos taurus ring finger protein 220 (RNF220), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa232986
Clone ID Related Accession (Same CDS sequence)	XM_024989713.1
Accession Version	XM_024989713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGGG CGGGAGCCTG GCTGCCAGCG GCTCGGGGCT CAGAGTTCTC CTCTCCAAGG 
TGGGCCCCTC GCCAGACTCC TCTCTCTGGG GGAAGCAAGC ACACTTGGAG CAAGAACTCC 
CTTCTGAAGG ATGCCATGGC TCCAGGCACC CCCAAGTCCC TCTTGTTGTC CGCTTCCATC 
AAGAGGGAAG GAGAGTCTCC AACGGCATCA CCACACTCGT CAGCCACCGA CGACCTGCAC 
CACTCAGACA GATACCAGAC CTTCTTGCGA GTGCGAGCCA ACCGGCAGAC CCGACTGAAT 
GCTCGGATTG GGAAAATGAA ACGGAGGAAG CAAGATGAAG GGCAGGTATG TCCCCTGTGC 
AGCCGCCCCC TGGCAGGATC GGAGCAGGAG ATGAGTAGGC ATGTGGAGCA TTGCCTTTCT 
AAGAGGGAAG GCTCCTGCAT GGCTGAGGAC GATGCCGCGG ACATCGAGCA TGAGAACAGC 
AACCGCTTTG AGGAGTATGA GTGGTGCGGG CAGAAGCGGA TACGGGCCAC CACTCTCCTG 
GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC ATGTGCAGCG GCAAGGAGAA TCCGGACAGT 
GACGCTGACT TGGATGTGGA TGGGGATGAC ACTCTGGAAT ATGGGAAGCC ACAATACACG 
GAGGCCGATG TCATCCCCTG CACAGGCGAG GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA 
CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT CCTAGCACAC GCATCACACC TGAGTTCTCT 
AAATGGGCCA GTGATGAGAT GCCATCTACC AGCAACGGCG AGAGCAGCAA ACAGGAGGCC 
ATGCAGAAGA CCTGCAAGAA CAGCGACATT GAGAAAATCA CCGAAGATTC AGCTGTGACT 
ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG GAACTTGAAC GGCAGCTATC TCGTGGGGAC 
CGTTACAAAT GCCTCATCTG CATGGACTCA TACTCGATGC CCCTGACATC CATCCAGTGT 
TGGCATGTGC ACTGTGAGGA GTGCTGGCTA CGGACCCTGG GCGCCAAGAA GCTCTGCCCC 
CAGTGCAACA CCATCACAGC GCCTGGAGAC CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024845481.1
CDS	16..1146
Translation	MGWAGAWLPAARGSEFSSPRWAPRQTPLSGGSKHTWSKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQVCPLCSRPLAGSEQEMSRHVEHCLSKREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_024989713.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024989713.1

ORF Insert Sequence:

ATGGGCTGGG CGGGAGCCTG GCTGCCAGCG GCTCGGGGCT CAGAGTTCTC CTCTCCAAGG 
TGGGCCCCTC GCCAGACTCC TCTCTCTGGG GGAAGCAAGC ACACTTGGAG CAAGAACTCC 
CTTCTGAAGG ATGCCATGGC TCCAGGCACC CCCAAGTCCC TCTTGTTGTC CGCTTCCATC 
AAGAGGGAAG GAGAGTCTCC AACGGCATCA CCACACTCGT CAGCCACCGA CGACCTGCAC 
CACTCAGACA GATACCAGAC CTTCTTGCGA GTGCGAGCCA ACCGGCAGAC CCGACTGAAT 
GCTCGGATTG GGAAAATGAA ACGGAGGAAG CAAGATGAAG GGCAGGTATG TCCCCTGTGC 
AGCCGCCCCC TGGCAGGATC GGAGCAGGAG ATGAGTAGGC ATGTGGAGCA TTGCCTTTCT 
AAGAGGGAAG GCTCCTGCAT GGCTGAGGAC GATGCCGCGG ACATCGAGCA TGAGAACAGC 
AACCGCTTTG AGGAGTATGA GTGGTGCGGG CAGAAGCGGA TACGGGCCAC CACTCTCCTG 
GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC ATGTGCAGCG GCAAGGAGAA TCCGGACAGT 
GACGCTGACT TGGATGTGGA TGGGGATGAC ACTCTGGAAT ATGGGAAGCC ACAATACACG 
GAGGCCGATG TCATCCCCTG CACAGGCGAG GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA 
CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT CCTAGCACAC GCATCACACC TGAGTTCTCT 
AAATGGGCCA GTGATGAGAT GCCATCTACC AGCAACGGCG AGAGCAGCAA ACAGGAGGCC 
ATGCAGAAGA CCTGCAAGAA CAGCGACATT GAGAAAATCA CCGAAGATTC AGCTGTGACT 
ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG GAACTTGAAC GGCAGCTATC TCGTGGGGAC 
CGTTACAAAT GCCTCATCTG CATGGACTCA TACTCGATGC CCCTGACATC CATCCAGTGT 
TGGCATGTGC ACTGTGAGGA GTGCTGGCTA CGGACCCTGG GCGCCAAGAA GCTCTGCCCC 
CAGTGCAACA CCATCACAGC GCCTGGAGAC CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_010803710.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802012.1 Bos taurus ring finger protein 220 (RNF220), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa232984
Clone ID Related Accession (Same CDS sequence)	XM_010803710.3 , XM_005204860.4
Accession Version	XM_010803710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802012.1
CDS	396..2174
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPASKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQVCPLCSRPLAGSEQEMSRHVEHCLSKREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_010803710.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803710.3

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_005204860.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005204917.1 Bos taurus ring finger protein 220 (RNF220), transcript variant X2, mRNA.

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CloneID	OBa232984
Clone ID Related Accession (Same CDS sequence)	XM_010803710.3 , XM_005204860.4
Accession Version	XM_005204860.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005204860.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005204917.1
CDS	367..2145
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPASKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQVCPLCSRPLAGSEQEMSRHVEHCLSKREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_005204860.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005204860.4

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGGTATGTC CCCTGTGCAG CCGCCCCCTG 
GCAGGATCGG AGCAGGAGAT GAGTAGGCAT GTGGAGCATT GCCTTTCTAA GAGGGAAGGC 
TCCTGCATGG CTGAGGACGA TGCCGCGGAC ATCGAGCATG AGAACAGCAA CCGCTTTGAG 
GAGTATGAGT GGTGCGGGCA GAAGCGGATA CGGGCCACCA CTCTCCTGGA AGGTGGTTTC 
CGAGGCTCTG GCTTCGTCAT GTGCAGCGGC AAGGAGAATC CGGACAGTGA CGCTGACTTG 
GATGTGGATG GGGATGACAC TCTGGAATAT GGGAAGCCAC AATACACGGA GGCCGATGTC 
ATCCCCTGCA CAGGCGAGGA GCCTGGCGAA GCCAAGGAGA GAGAGGCACT GCGGGGTGCA 
GTCCTAAATG GTGGCCCTCC TAGCACACGC ATCACACCTG AGTTCTCTAA ATGGGCCAGT 
GATGAGATGC CATCTACCAG CAACGGCGAG AGCAGCAAAC AGGAGGCCAT GCAGAAGACC 
TGCAAGAACA GCGACATTGA GAAAATCACC GAAGATTCAG CTGTGACTAC GTTTGAGGCC 
CTGAAAGCTC GGGTCAGGGA ACTTGAACGG CAGCTATCTC GTGGGGACCG TTACAAATGC 
CTCATCTGCA TGGACTCATA CTCGATGCCC CTGACATCCA TCCAGTGTTG GCATGTGCAC 
TGTGAGGAGT GCTGGCTACG GACCCTGGGC GCCAAGAAGC TCTGCCCCCA GTGCAACACC 
ATCACAGCGC CTGGAGACCT GCGGAGAATC TACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF220 ( XM_010803713.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802015.1 Bos taurus ring finger protein 220 (RNF220), transcript variant X3, mRNA.

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CloneID	OBa232985
Clone ID Related Accession (Same CDS sequence)	XM_010803713.3
Accession Version	XM_010803713.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase RNF220 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCTGAGGAC 
GATGCCGCGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
CAGAAGCGGA TACGGGCCAC CACTCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
ATGTGCAGCG GCAAGGAGAA TCCGGACAGT GACGCTGACT TGGATGTGGA TGGGGATGAC 
ACTCTGGAAT ATGGGAAGCC ACAATACACG GAGGCCGATG TCATCCCCTG CACAGGCGAG 
GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT 
CCTAGCACAC GCATCACACC TGAGTTCTCT AAATGGGCCA GTGATGAGAT GCCATCTACC 
AGCAACGGCG AGAGCAGCAA ACAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATT 
GAGAAAATCA CCGAAGATTC AGCTGTGACT ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG 
GAACTTGAAC GGCAGCTATC TCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCA 
TACTCGATGC CCCTGACATC CATCCAGTGT TGGCATGTGC ACTGTGAGGA GTGCTGGCTA 
CGGACCCTGG GCGCCAAGAA GCTCTGCCCC CAGTGCAACA CCATCACAGC GCCTGGAGAC 
CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802015.1
CDS	396..2096
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDTGKKIFAVSGLISDREASSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPASKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQTFLRVRANRQTRLNARIGKMKRRKQDEGQREGSCMAEDDAADIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADVIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_010803713.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 220 (RNF220), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803713.3

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCAT CCTACCTCCC CAACCCGCTG 
GCATCTCCAG CCCTGATGGT CCTGGCGTCC ACGGCCGAGG CTAGCCGTGA TGCATCCATC 
CCTTGTCAGC AGCCACGACC CTTCGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCATATT 
CCGTTCACCA ATGGTTCCTA CACCTTTGCC TCTATGTACC ATCGGCAAGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCATTGCTAC ACCTCCACCC GCAGTTTGCT 
CCCCCAAACC TAGATTGCAC CCCAATCAGT ATGCTGAATC ACAGCGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTATCAGT CAGCCTTCAC GCCCGCCAAG 
CGACTTAAAA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC ACAGCTGCCA TCTAGCTCCC CCGGTTCACT TAAGGTTGAC 
GACACTGGGA AGAAGATTTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AGCCTCATCT 
AGCCCAGAAG ATCGGAATGA CAGATGTAAG AAAAAGGCAG CAGCACTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG TCAGGTCCTG CTGAGGCCCA GCGAGCTACA GGAGCATATG 
GAGCAAGAAC TAGAGCAGCT GGCCCAGCTG CCCGCCAGCA AGAACTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGCACCCC CAAGTCCCTC TTGTTGTCCG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCAA CGGCATCACC ACACTCGTCA GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGACCT TCTTGCGAGT GCGAGCCAAC CGGCAGACCC GACTGAATGC TCGGATTGGG 
AAAATGAAAC GGAGGAAGCA AGATGAAGGG CAGAGGGAAG GCTCCTGCAT GGCTGAGGAC 
GATGCCGCGG ACATCGAGCA TGAGAACAGC AACCGCTTTG AGGAGTATGA GTGGTGCGGG 
CAGAAGCGGA TACGGGCCAC CACTCTCCTG GAAGGTGGTT TCCGAGGCTC TGGCTTCGTC 
ATGTGCAGCG GCAAGGAGAA TCCGGACAGT GACGCTGACT TGGATGTGGA TGGGGATGAC 
ACTCTGGAAT ATGGGAAGCC ACAATACACG GAGGCCGATG TCATCCCCTG CACAGGCGAG 
GAGCCTGGCG AAGCCAAGGA GAGAGAGGCA CTGCGGGGTG CAGTCCTAAA TGGTGGCCCT 
CCTAGCACAC GCATCACACC TGAGTTCTCT AAATGGGCCA GTGATGAGAT GCCATCTACC 
AGCAACGGCG AGAGCAGCAA ACAGGAGGCC ATGCAGAAGA CCTGCAAGAA CAGCGACATT 
GAGAAAATCA CCGAAGATTC AGCTGTGACT ACGTTTGAGG CCCTGAAAGC TCGGGTCAGG 
GAACTTGAAC GGCAGCTATC TCGTGGGGAC CGTTACAAAT GCCTCATCTG CATGGACTCA 
TACTCGATGC CCCTGACATC CATCCAGTGT TGGCATGTGC ACTGTGAGGA GTGCTGGCTA 
CGGACCCTGG GCGCCAAGAA GCTCTGCCCC CAGTGCAACA CCATCACAGC GCCTGGAGAC 
CTGCGGAGAA TCTACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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RNF220 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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