PUS10 cDNA ORF clone, Bos taurus(cattle)

The following PUS10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUS10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa40926 NM_001206837.1
Latest version!
Bos taurus pseudouridine synthase 10 (PUS10), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa23968 XM_010809989.2
Latest version!
Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa40926 XM_024999156.1
Latest version!
Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa40926 XM_024999154.1
Latest version!
Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa40926 XM_024999155.1
Latest version!
Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa220628 XM_024999157.1
Latest version!
Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa40926
    Clone ID Related Accession (Same CDS sequence) NM_001206837.1 , XM_024999154.1 , XM_024999156.1 , XM_024999155.1
    Accession Version NM_001206837.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-14
    Organism Bos taurus(cattle)
    Product putative tRNA pseudouridine synthase Pus10
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DY122709.1, NKLS02000011.1, EE373870.1, EH180952.1, BF076638.1, CB438385.1 and CO892503.1. On or before May 11, 2011 this sequence version replaced XM_002691333.1, XM_866656.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on homologous protein alignment with human NP_653310.2. ##Evidence-Data-START## CDS exon combination :: SRR6764281.1467.1, SRR5571255.3689.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193766.1
    CDS186..1784
    Translation

    Target ORF information:

    RefSeq Version NM_001206837.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pseudouridine synthase 10 (PUS10), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206837.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23968
    Clone ID Related Accession (Same CDS sequence) XM_010809989.2
    Accession Version XM_010809989.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product putative tRNA pseudouridine synthase Pus10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCA
    TCAAATAATA GATCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808291.1
    CDS416..2014
    Translation

    Target ORF information:

    RefSeq Version XM_010809989.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809989.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCA
    TCAAATAATA GATCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40926
    Clone ID Related Accession (Same CDS sequence) NM_001206837.1 , XM_024999154.1 , XM_024999156.1 , XM_024999155.1
    Accession Version XM_024999154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative tRNA pseudouridine synthase Pus10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854922.1
    CDS398..1996
    Translation

    Target ORF information:

    RefSeq Version XM_024999154.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40926
    Clone ID Related Accession (Same CDS sequence) NM_001206837.1 , XM_024999154.1 , XM_024999156.1 , XM_024999155.1
    Accession Version XM_024999156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative tRNA pseudouridine synthase Pus10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854924.1
    CDS153..1751
    Translation

    Target ORF information:

    RefSeq Version XM_024999156.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40926
    Clone ID Related Accession (Same CDS sequence) NM_001206837.1 , XM_024999154.1 , XM_024999156.1 , XM_024999155.1
    Accession Version XM_024999155.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative tRNA pseudouridine synthase Pus10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854923.1
    CDS41..1639
    Translation

    Target ORF information:

    RefSeq Version XM_024999155.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999155.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTTTCCTC TGACTGTGGA AAACAAGCAT GTGGCCCAGT TGTTGCTCAG TACTGGTACC 
    TGTCCAAGAT GTATCTTCAG ATTCTGTGGT GTGGATTTTC ATGCACCATA CAAACTTCCC
    TACAAGGAGT TGCTCCGTGA ACTACAAAAA TTTCTGGAAG CTGAAGAAGA TGAATTAGTT
    TCTGAAGTTC CAAACCCTCC TCCCAAGAAA ATTCGACTCC AAGAACCAGA AGATCAGATT
    GATGGGATGG ATAGTCTGAG TCAAAATGGA GAGGGAAAGC TCTCAGTGAC TGAAGATGGA
    AGCAGTTCTT CCAAGAACTC GAATTTAAAT GTATGCAATG TATGCCTAGG AATTCTTCAA
    GATTTCTGTG AAAAAGAGTT CATTAAAAAG GTGTGCCAGA AGGTTGAGGC CTCTGGGTTT
    GAATTCACCA ACTTGGTATT GTCAGTCTCC TTCCCACCGC AGCTATCTGT AAGAGAGCAT
    GCTGCTTGGT TGCTGGTAAA ACAGGAAATG GGAAAGCAGA GTCTGTCGCT GGGAAGAAAT
    GATATAGTTC AGCTAAAAGA AGCCTACAAA TGGATAACTC ACCCCCTGTT TTCAGAGGAA
    CTGGGTGTTC CCATTGATGG AAAGAGCTTA TTTGAAGTGA GTGTGGTCTT TGCTCACCCA
    GAAACAGTTG AGGATTGCCA CTTCCTACGT GCAATATGCC CAGATTGTTT CAAGCCAGCC
    AAAAACAAAC AGTCAGTATT CACTAGAATG GCAGTTATGA AAGCTTTGAA TAAGATAAAA
    GAAGAGGATT TCTGCAAGCA GTTTCCTTGT CCTCCAAACT CACCAAAGGC TGTATGCACT
    GTTCTTGAAA TTGAATGTGC TCATGGTGCT GTTTTTGTGG CTGGGAGATA TAATAAATAC
    TCCAGGAATC TACCACAAAC TCCTTGGATA ATTGATGGAG AAAGGAAGCT AGAATCTTCA
    GTAGAAGAAT TAATTTCAGA CCATCTTTTG AAAGTGTTCA AAGCAGAAAG CTTTAATTTT
    TCTTCCTCTG GAAGAGAAGA TGTAGATGTG AGAACATTAG GAAATGGAAG GCCCTTTGCA
    ATTGAGCTGG TGAATCCTCA TAGAGTACAT TTCACTTCAC AAGAAATTAA GGAACTTCAG
    CAGAAAATTA ATAACTCATC TAACAAAATC CAAGTCCGTG ACTTGCAGCT TGTCACAAGA
    GAGGCAATAG GACATATGAA AGAAGGTGAA GAAGAGAAGA CCAAGACCTA CAGTGCCTTA
    ATATGGACAA ATAAAGCAAT ACAGAGGAAG GACATTGAAT TCCTAGATGA CATAAAGGAC
    TTAAATATTG ACCAGAAAAC ACCTTTACGG GTCCTTCACC GGAGGCCCCT GGCTGTGAGA
    ACTCGCATCA TTCACTCCAT GGAGACACGC TATGTGGACG AGCACCATTT CCGCCTCTAC
    CTGAAGACTC AAGCTGGAAC CTACATTAAA GAGTTTGTAC ATGGAGACTT TGGAAGAACC
    AAGCCAAACA TTGGCTCTCT GATGGATGTG ACTGCAGACA TTCTTGAGCT GGATGTTGAG
    TCTGTAGATG TTGACTGGCC CCCTGCTCTG GATGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa220628
    Clone ID Related Accession (Same CDS sequence) XM_024999157.1
    Accession Version XM_024999157.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product putative tRNA pseudouridine synthase Pus10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGATAGTC TGAGTCAAAA TGGAGAGGGA AAGCTCTCAG TGACTGAAGA TGGAAGCAGT 
    TCTTCCAAGA ACTCGAATTT AAATGTATGC AATGTATGCC TAGGAATTCT TCAAGATTTC
    TGTGAAAAAG AGTTCATTAA AAAGGTGTGC CAGAAGGTTG AGGCCTCTGG GTTTGAATTC
    ACCAACTTGG TATTGTCAGT CTCCTTCCCA CCGCAGCTAT CTGTAAGAGA GCATGCTGCT
    TGGTTGCTGG TAAAACAGGA AATGGGAAAG CAGAGTCTGT CGCTGGGAAG AAATGATATA
    GTTCAGCTAA AAGAAGCCTA CAAATGGATA ACTCACCCCC TGTTTTCAGA GGAACTGGGT
    GTTCCCATTG ATGGAAAGAG CTTATTTGAA GTGAGTGTGG TCTTTGCTCA CCCAGAAACA
    GTTGAGGATT GCCACTTCCT ACGTGCAATA TGCCCAGATT GTTTCAAGCC AGCCAAAAAC
    AAACAGTCAG TATTCACTAG AATGGCAGTT ATGAAAGCTT TGAATAAGAT AAAAGAAGAG
    GATTTCTGCA AGCAGTTTCC TTGTCCTCCA AACTCACCAA AGGCTGTATG CACTGTTCTT
    GAAATTGAAT GTGCTCATGG TGCTGTTTTT GTGGCTGGGA GATATAATAA ATACTCCAGG
    AATCTACCAC AAACTCCTTG GATAATTGAT GGAGAAAGGA AGCTAGAATC TTCAGTAGAA
    GAATTAATTT CAGACCATCT TTTGAAAGTG TTCAAAGCAG AAAGCTTTAA TTTTTCTTCC
    TCTGGAAGAG AAGATGTAGA TGTGAGAACA TTAGGAAATG GAAGGCCCTT TGCAATTGAG
    CTGGTGAATC CTCATAGAGT ACATTTCACT TCACAAGAAA TTAAGGAACT TCAGCAGAAA
    ATTAATAACT CATCTAACAA AATCCAAGTC CGTGACTTGC AGCTTGTCAC AAGAGAGGCA
    ATAGGACATA TGAAAGAAGG TGAAGAAGAG AAGACCAAGA CCTACAGTGC CTTAATATGG
    ACAAATAAAG CAATACAGAG GAAGGACATT GAATTCCTAG ATGACATAAA GGACTTAAAT
    ATTGACCAGA AAACACCTTT ACGGGTCCTT CACCGGAGGC CCCTGGCTGT GAGAACTCGC
    ATCATTCACT CCATGGAGAC ACGCTATGTG GACGAGCACC ATTTCCGCCT CTACCTGAAG
    ACTCAAGCTG GAACCTACAT TAAAGAGTTT GTACATGGAG ACTTTGGAAG AACCAAGCCA
    AACATTGGCT CTCTGATGGA TGTGACTGCA GACATTCTTG AGCTGGATGT TGAGTCTGTA
    GATGTTGACT GGCCCCCTGC TCTGGATGAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854925.1
    CDS494..1846
    Translation

    Target ORF information:

    RefSeq Version XM_024999157.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pseudouridylate synthase 10 (PUS10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999157.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGATAGTC TGAGTCAAAA TGGAGAGGGA AAGCTCTCAG TGACTGAAGA TGGAAGCAGT 
    TCTTCCAAGA ACTCGAATTT AAATGTATGC AATGTATGCC TAGGAATTCT TCAAGATTTC
    TGTGAAAAAG AGTTCATTAA AAAGGTGTGC CAGAAGGTTG AGGCCTCTGG GTTTGAATTC
    ACCAACTTGG TATTGTCAGT CTCCTTCCCA CCGCAGCTAT CTGTAAGAGA GCATGCTGCT
    TGGTTGCTGG TAAAACAGGA AATGGGAAAG CAGAGTCTGT CGCTGGGAAG AAATGATATA
    GTTCAGCTAA AAGAAGCCTA CAAATGGATA ACTCACCCCC TGTTTTCAGA GGAACTGGGT
    GTTCCCATTG ATGGAAAGAG CTTATTTGAA GTGAGTGTGG TCTTTGCTCA CCCAGAAACA
    GTTGAGGATT GCCACTTCCT ACGTGCAATA TGCCCAGATT GTTTCAAGCC AGCCAAAAAC
    AAACAGTCAG TATTCACTAG AATGGCAGTT ATGAAAGCTT TGAATAAGAT AAAAGAAGAG
    GATTTCTGCA AGCAGTTTCC TTGTCCTCCA AACTCACCAA AGGCTGTATG CACTGTTCTT
    GAAATTGAAT GTGCTCATGG TGCTGTTTTT GTGGCTGGGA GATATAATAA ATACTCCAGG
    AATCTACCAC AAACTCCTTG GATAATTGAT GGAGAAAGGA AGCTAGAATC TTCAGTAGAA
    GAATTAATTT CAGACCATCT TTTGAAAGTG TTCAAAGCAG AAAGCTTTAA TTTTTCTTCC
    TCTGGAAGAG AAGATGTAGA TGTGAGAACA TTAGGAAATG GAAGGCCCTT TGCAATTGAG
    CTGGTGAATC CTCATAGAGT ACATTTCACT TCACAAGAAA TTAAGGAACT TCAGCAGAAA
    ATTAATAACT CATCTAACAA AATCCAAGTC CGTGACTTGC AGCTTGTCAC AAGAGAGGCA
    ATAGGACATA TGAAAGAAGG TGAAGAAGAG AAGACCAAGA CCTACAGTGC CTTAATATGG
    ACAAATAAAG CAATACAGAG GAAGGACATT GAATTCCTAG ATGACATAAA GGACTTAAAT
    ATTGACCAGA AAACACCTTT ACGGGTCCTT CACCGGAGGC CCCTGGCTGT GAGAACTCGC
    ATCATTCACT CCATGGAGAC ACGCTATGTG GACGAGCACC ATTTCCGCCT CTACCTGAAG
    ACTCAAGCTG GAACCTACAT TAAAGAGTTT GTACATGGAG ACTTTGGAAG AACCAAGCCA
    AACATTGGCT CTCTGATGGA TGTGACTGCA GACATTCTTG AGCTGGATGT TGAGTCTGTA
    GATGTTGACT GGCCCCCTGC TCTGGATGAC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.