NAE1 cDNA ORF clone, Bos taurus(cattle)

The following NAE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa225154 XM_024978483.1
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa225155 XM_024978484.1
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa28503 XM_015475771.1
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa28503 XM_005218714.3
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa28503 XM_024978485.1
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa28503 XM_015475772.1
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa36734 NM_001193033.1
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa28502 XM_005218713.4
Latest version!
Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa28502 XM_005218713.3 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa225154
    Clone ID Related Accession (Same CDS sequence) XM_024978483.1
    Accession Version XM_024978483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT
    CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT
    CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT
    CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT
    AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC
    AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA
    GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT
    AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTCTT TTTGTTGTTC
    TTTTCCAGTT ACCGTGAAAA AGCAAAGAAA GATGCTGCTG CTGTGGGTAA TCATGTTGCC
    AAATTGCTTC AGTCTATAGG CCAGGCACCA GAGTCCATTT CAGAGAAAGA ATTAAAATTA
    CTCTGCAGCA ATTCAGCATT TCTTCGAGTG GTGAGATGTC GATCCTTAGC TGAAGAATAC
    AGCTTGGATA CAATTAACAA GGATGAAATT ATTTCCAGCA TGGACAATCC AGATAACGAG
    ATAGTATTAT ACTTAATGTT ACGGGCTGTT GATAGATTTC ATAAACAGCA TGGTAGATAT
    CCAGGAGTGT CTAACTATCA AGTTGAAGAA GATATAGGAA AGTTGAAGTC TTGTCTCACT
    AGCTTCCTTC AGGAATATGG ATTATCTGTA ATGGTGAAAG ACGATTATGT CCATGAATTT
    TGCCGATACG GAGCTGCTGA ACCACACACC ATTGCTGCAT TCTTAGGGGG AGCTGCTGCT
    CAAGAGGTTA TCAAAATAAT CACCAAACAA TTTGTAATTT TTAATAATAC TTACATTTAT
    AGTGGCATGT CACAAACTTC AGCAACTTTT CAGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834251.1
    CDS51..1409
    Translation

    Target ORF information:

    RefSeq Version XM_024978483.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT
    CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT
    CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT
    CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT
    AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC
    AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA
    GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT
    AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTCTT TTTGTTGTTC
    TTTTCCAGTT ACCGTGAAAA AGCAAAGAAA GATGCTGCTG CTGTGGGTAA TCATGTTGCC
    AAATTGCTTC AGTCTATAGG CCAGGCACCA GAGTCCATTT CAGAGAAAGA ATTAAAATTA
    CTCTGCAGCA ATTCAGCATT TCTTCGAGTG GTGAGATGTC GATCCTTAGC TGAAGAATAC
    AGCTTGGATA CAATTAACAA GGATGAAATT ATTTCCAGCA TGGACAATCC AGATAACGAG
    ATAGTATTAT ACTTAATGTT ACGGGCTGTT GATAGATTTC ATAAACAGCA TGGTAGATAT
    CCAGGAGTGT CTAACTATCA AGTTGAAGAA GATATAGGAA AGTTGAAGTC TTGTCTCACT
    AGCTTCCTTC AGGAATATGG ATTATCTGTA ATGGTGAAAG ACGATTATGT CCATGAATTT
    TGCCGATACG GAGCTGCTGA ACCACACACC ATTGCTGCAT TCTTAGGGGG AGCTGCTGCT
    CAAGAGGTTA TCAAAATAAT CACCAAACAA TTTGTAATTT TTAATAATAC TTACATTTAT
    AGTGGCATGT CACAAACTTC AGCAACTTTT CAGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225155
    Clone ID Related Accession (Same CDS sequence) XM_024978484.1
    Accession Version XM_024978484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT
    CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT
    CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT
    CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT
    AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC
    AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA
    GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT
    AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTTTA CCGTGAAAAA
    GCAAAGAAAG ATGCTGCTGC TGTGGGTAAT CATGTTGCCA AATTGCTTCA GTCTATAGGC
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCAGCATTT
    CTTCGAGTGG TGAGATGTCG ATCCTTAGCT GAAGAATACA GCTTGGATAC AATTAACAAG
    GATGAAATTA TTTCCAGCAT GGACAATCCA GATAACGAGA TAGTATTATA CTTAATGTTA
    CGGGCTGTTG ATAGATTTCA TAAACAGCAT GGTAGATATC CAGGAGTGTC TAACTATCAA
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTA GCTTCCTTCA GGAATATGGA
    TTATCTGTAA TGGTGAAAGA CGATTATGTC CATGAATTTT GCCGATACGG AGCTGCTGAA
    CCACACACCA TTGCTGCATT CTTAGGGGGA GCTGCTGCTC AAGAGGTTAT CAAAATAATC
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTATA GTGGCATGTC ACAAACTTCA
    GCAACTTTTC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834252.1
    CDS292..1629
    Translation

    Target ORF information:

    RefSeq Version XM_024978484.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
    GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT
    CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT
    CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT
    CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT
    CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT
    AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC
    AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA
    GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT
    AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT
    AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT
    GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT
    CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTTTA CCGTGAAAAA
    GCAAAGAAAG ATGCTGCTGC TGTGGGTAAT CATGTTGCCA AATTGCTTCA GTCTATAGGC
    CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCAGCATTT
    CTTCGAGTGG TGAGATGTCG ATCCTTAGCT GAAGAATACA GCTTGGATAC AATTAACAAG
    GATGAAATTA TTTCCAGCAT GGACAATCCA GATAACGAGA TAGTATTATA CTTAATGTTA
    CGGGCTGTTG ATAGATTTCA TAAACAGCAT GGTAGATATC CAGGAGTGTC TAACTATCAA
    GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTA GCTTCCTTCA GGAATATGGA
    TTATCTGTAA TGGTGAAAGA CGATTATGTC CATGAATTTT GCCGATACGG AGCTGCTGAA
    CCACACACCA TTGCTGCATT CTTAGGGGGA GCTGCTGCTC AAGAGGTTAT CAAAATAATC
    ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTATA GTGGCATGTC ACAAACTTCA
    GCAACTTTTC AGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28503
    Clone ID Related Accession (Same CDS sequence) XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
    Accession Version XM_005218714.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218771.1
    CDS334..1620
    Translation

    Target ORF information:

    RefSeq Version XM_005218714.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218714.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28503
    Clone ID Related Accession (Same CDS sequence) XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
    Accession Version XM_024978485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834253.1
    CDS329..1615
    Translation

    Target ORF information:

    RefSeq Version XM_024978485.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28503
    Clone ID Related Accession (Same CDS sequence) XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
    Accession Version XM_015475771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331257.1
    CDS1434..2720
    Translation

    Target ORF information:

    RefSeq Version XM_015475771.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28503
    Clone ID Related Accession (Same CDS sequence) XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
    Accession Version XM_015475772.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331258.1
    CDS835..2121
    Translation

    Target ORF information:

    RefSeq Version XM_015475772.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
    AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT
    AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA
    TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA
    GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA
    TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA
    CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG
    AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT
    ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC
    TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA
    TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT
    GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC
    CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG
    TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT
    TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA
    ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC
    TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT
    AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG
    GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA
    GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC
    AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA
    CAAACTTCAG CAACTTTTCA GTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36734
    Clone ID Related Accession (Same CDS sequence) NM_001193033.1
    Accession Version NM_001193033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000018.1. On Jul 17, 2010 this sequence version replaced XM_866024.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571257.29905.1, SRR5571263.18581.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC
    ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC
    TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC
    TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA
    GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA
    GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT
    TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA
    TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA
    GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG
    ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA
    GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA
    AAACTTCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT
    GTTGCCAAAT TGCTTCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA
    AAATTACTCT GCAGCAATTC AGCATTTCTT CGAGTGGTGA GATGTCGATC CTTAGCTGAA
    GAATACAGCT TGGATACAAT TAACAAGGAT GAAATTATTT CCAGCATGGA CAATCCAGAT
    AACGAGATAG TATTATACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAGCATGGT
    AGATATCCAG GAGTGTCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT
    CTCACTAGCT TCCTTCAGGA ATATGGATTA TCTGTAATGG TGAAAGACGA TTATGTCCAT
    GAATTTTGCC GATACGGAGC TGCTGAACCA CACACCATTG CTGCATTCTT AGGGGGAGCT
    GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC
    ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179962.1
    CDS58..1662
    Translation

    Target ORF information:

    RefSeq Version NM_001193033.1
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC
    ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC
    TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC
    TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA
    GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA
    GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT
    TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA
    TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA
    GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG
    ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA
    GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA
    AAACTTCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT
    GTTGCCAAAT TGCTTCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA
    AAATTACTCT GCAGCAATTC AGCATTTCTT CGAGTGGTGA GATGTCGATC CTTAGCTGAA
    GAATACAGCT TGGATACAAT TAACAAGGAT GAAATTATTT CCAGCATGGA CAATCCAGAT
    AACGAGATAG TATTATACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAGCATGGT
    AGATATCCAG GAGTGTCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT
    CTCACTAGCT TCCTTCAGGA ATATGGATTA TCTGTAATGG TGAAAGACGA TTATGTCCAT
    GAATTTTGCC GATACGGAGC TGCTGAACCA CACACCATTG CTGCATTCTT AGGGGGAGCT
    GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC
    ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28502
    Clone ID Related Accession (Same CDS sequence) XM_005218713.3 , XM_005218713.4
    Accession Version XM_005218713.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC
    ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC
    TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC
    TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA
    GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA
    GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT
    TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA
    TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA
    GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG
    ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA
    GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA
    AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT
    GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG
    TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG
    AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT
    TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT
    AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT
    ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG
    GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT
    GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT
    GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218770.1
    CDS74..1699
    Translation

    Target ORF information:

    RefSeq Version XM_005218713.3
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218713.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC
    ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC
    TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC
    TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA
    GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA
    GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT
    TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA
    TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA
    GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG
    ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA
    GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA
    AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT
    GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG
    TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG
    AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT
    TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT
    AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT
    ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG
    GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT
    GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT
    GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa28502
    Clone ID Related Accession (Same CDS sequence) XM_005218713.3 , XM_005218713.4
    Accession Version XM_005218713.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD8-activating enzyme E1 regulatory subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218713.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC
    ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC
    TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC
    TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA
    GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA
    GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT
    TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA
    TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA
    GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG
    ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA
    GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA
    AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT
    GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG
    TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG
    AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT
    TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT
    AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT
    ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG
    GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT
    GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT
    GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005218770.1
    CDS106..1731
    Translation

    Target ORF information:

    RefSeq Version XM_005218713.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005218713.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
    GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC
    ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT
    GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT
    ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC
    TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC
    TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA
    GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT
    TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA
    GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT
    TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA
    TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA
    GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA
    GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG
    ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG
    ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA
    GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA
    AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT
    GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG
    TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG
    AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT
    TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT
    AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT
    ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG
    GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT
    GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT
    GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG
    TTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.