NAE1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
NM_001193033.1	NP_001179962.1	NEDD8-activating enzyme E1 regulatory subunit
XM_024978483.1	XP_024834251.1	NEDD8-activating enzyme E1 regulatory subunit isoform X2
XM_024978484.1	XP_024834252.1	NEDD8-activating enzyme E1 regulatory subunit isoform X3
XM_005218713.3	XP_005218770.1	NEDD8-activating enzyme E1 regulatory subunit isoform X1
XM_005218714.3	XP_005218771.1	NEDD8-activating enzyme E1 regulatory subunit isoform X4
XM_024978485.1	XP_024834253.1	NEDD8-activating enzyme E1 regulatory subunit isoform X4
XM_015475771.1	XP_015331257.1	NEDD8-activating enzyme E1 regulatory subunit isoform X2
XM_005218713.4	XP_005218770.1	NEDD8-activating enzyme E1 regulatory subunit isoform X1
XM_015475772.1	XP_015331258.1	NEDD8-activating enzyme E1 regulatory subunit isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225154	XM_024978483.1 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa225155	XM_024978484.1 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa28503	XM_015475771.1 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa28503	XM_005218714.3 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa28503	XM_024978485.1 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa28503	XM_015475772.1 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa36734	NM_001193033.1 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa28502	XM_005218713.4 Latest version!	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa28502	XM_005218713.3	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

NAE1 ( XM_024978483.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834251.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa225154
Clone ID Related Accession (Same CDS sequence)	XM_024978483.1
Accession Version	XM_024978483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT 
CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT 
AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC 
AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA 
GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTCTT TTTGTTGTTC 
TTTTCCAGTT ACCGTGAAAA AGCAAAGAAA GATGCTGCTG CTGTGGGTAA TCATGTTGCC 
AAATTGCTTC AGTCTATAGG CCAGGCACCA GAGTCCATTT CAGAGAAAGA ATTAAAATTA 
CTCTGCAGCA ATTCAGCATT TCTTCGAGTG GTGAGATGTC GATCCTTAGC TGAAGAATAC 
AGCTTGGATA CAATTAACAA GGATGAAATT ATTTCCAGCA TGGACAATCC AGATAACGAG 
ATAGTATTAT ACTTAATGTT ACGGGCTGTT GATAGATTTC ATAAACAGCA TGGTAGATAT 
CCAGGAGTGT CTAACTATCA AGTTGAAGAA GATATAGGAA AGTTGAAGTC TTGTCTCACT 
AGCTTCCTTC AGGAATATGG ATTATCTGTA ATGGTGAAAG ACGATTATGT CCATGAATTT 
TGCCGATACG GAGCTGCTGA ACCACACACC ATTGCTGCAT TCTTAGGGGG AGCTGCTGCT 
CAAGAGGTTA TCAAAATAAT CACCAAACAA TTTGTAATTT TTAATAATAC TTACATTTAT 
AGTGGCATGT CACAAACTTC AGCAACTTTT CAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834251.1
CDS	51..1409
Translation	MEFLQELNNDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVFLLFFSSYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_024978483.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978483.1

ORF Insert Sequence:

ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT 
CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT 
AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC 
AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA 
GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTCTT TTTGTTGTTC 
TTTTCCAGTT ACCGTGAAAA AGCAAAGAAA GATGCTGCTG CTGTGGGTAA TCATGTTGCC 
AAATTGCTTC AGTCTATAGG CCAGGCACCA GAGTCCATTT CAGAGAAAGA ATTAAAATTA 
CTCTGCAGCA ATTCAGCATT TCTTCGAGTG GTGAGATGTC GATCCTTAGC TGAAGAATAC 
AGCTTGGATA CAATTAACAA GGATGAAATT ATTTCCAGCA TGGACAATCC AGATAACGAG 
ATAGTATTAT ACTTAATGTT ACGGGCTGTT GATAGATTTC ATAAACAGCA TGGTAGATAT 
CCAGGAGTGT CTAACTATCA AGTTGAAGAA GATATAGGAA AGTTGAAGTC TTGTCTCACT 
AGCTTCCTTC AGGAATATGG ATTATCTGTA ATGGTGAAAG ACGATTATGT CCATGAATTT 
TGCCGATACG GAGCTGCTGA ACCACACACC ATTGCTGCAT TCTTAGGGGG AGCTGCTGCT 
CAAGAGGTTA TCAAAATAAT CACCAAACAA TTTGTAATTT TTAATAATAC TTACATTTAT 
AGTGGCATGT CACAAACTTC AGCAACTTTT CAGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_024978484.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834252.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa225155
Clone ID Related Accession (Same CDS sequence)	XM_024978484.1
Accession Version	XM_024978484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT 
CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT 
AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC 
AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA 
GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCTGC TGTGGGTAAT CATGTTGCCA AATTGCTTCA GTCTATAGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCAGCATTT 
CTTCGAGTGG TGAGATGTCG ATCCTTAGCT GAAGAATACA GCTTGGATAC AATTAACAAG 
GATGAAATTA TTTCCAGCAT GGACAATCCA GATAACGAGA TAGTATTATA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAGCAT GGTAGATATC CAGGAGTGTC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTA GCTTCCTTCA GGAATATGGA 
TTATCTGTAA TGGTGAAAGA CGATTATGTC CATGAATTTT GCCGATACGG AGCTGCTGAA 
CCACACACCA TTGCTGCATT CTTAGGGGGA GCTGCTGCTC AAGAGGTTAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTATA GTGGCATGTC ACAAACTTCA 
GCAACTTTTC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834252.1
CDS	292..1629
Translation	MEFLQELNNDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_024978484.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978484.1

ORF Insert Sequence:

ATGGAATTCT TACAAGAATT AAATAACGAT GTCTCTGGGA GTTTTGTGGA AGAGAGTCCA 
GAAAACCTTC TAGACAATGA TCCTTCATTT TTCTGTAGGT TTACCATTGT GGTTGCAACT 
CAGCTTTCTG AAAGTACATT ACTACGTTTA GCAGATGTCC TCTGGAATTC CCAAATCCCT 
CTTTTGATCT GTAGGACATA TGGACTAGTT GGTTATATGA GGATCATTAT AAAAGAACAT 
CCAGTAATAG AATCTCATCC AGATAATGCC TTAGAGGATC TACGACTGGA TAAGCCATTT 
CCTGAACTGA GAGAACATTT TCAGTCTTAC GATTTGGATC ATATGGAAAA AAAGGACCAT 
AGCCACACTC CATGGATTGT GATCGTAGCT AAATACTTAG CACAGTGGTA TAGTGAAACC 
AATGGACGAA TACCTAAAAC ATATAAAGAA AAAGAAGACT TCAGAGATTT GATTAGACAA 
GGAATTTTAA AGAATGAAAA TGGGACTCCA GAAGATGAAG AGAATTTTGA AGAAGCTATT 
AAAAATGTGA ATACAGCACT AAATACAACT CAGATCCCAA GCAGTATTGA AGATATATTT 
AATGATGATC GCTGCATAAA TATCACCAAA CAGACTCCGA CATTTTGGAT TTTAGCTCGT 
GCCTTAAAGG AATTTGTGGC CAAAGAGGGT CAAGGAAATC TACCTGTTCG AGGCACAATT 
CCTGATATGA TTGCAGATTC AGGCAAATAT ATAAAACTTC AAAATGTTTA CCGTGAAAAA 
GCAAAGAAAG ATGCTGCTGC TGTGGGTAAT CATGTTGCCA AATTGCTTCA GTCTATAGGC 
CAGGCACCAG AGTCCATTTC AGAGAAAGAA TTAAAATTAC TCTGCAGCAA TTCAGCATTT 
CTTCGAGTGG TGAGATGTCG ATCCTTAGCT GAAGAATACA GCTTGGATAC AATTAACAAG 
GATGAAATTA TTTCCAGCAT GGACAATCCA GATAACGAGA TAGTATTATA CTTAATGTTA 
CGGGCTGTTG ATAGATTTCA TAAACAGCAT GGTAGATATC CAGGAGTGTC TAACTATCAA 
GTTGAAGAAG ATATAGGAAA GTTGAAGTCT TGTCTCACTA GCTTCCTTCA GGAATATGGA 
TTATCTGTAA TGGTGAAAGA CGATTATGTC CATGAATTTT GCCGATACGG AGCTGCTGAA 
CCACACACCA TTGCTGCATT CTTAGGGGGA GCTGCTGCTC AAGAGGTTAT CAAAATAATC 
ACCAAACAAT TTGTAATTTT TAATAATACT TACATTTATA GTGGCATGTC ACAAACTTCA 
GCAACTTTTC AGTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_005218714.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218771.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa28503
Clone ID Related Accession (Same CDS sequence)	XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
Accession Version	XM_005218714.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218714.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218771.1
CDS	334..1620
Translation	MSLGVLWKRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVFLLFFSSYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_005218714.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218714.3

ORF Insert Sequence:

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_024978485.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834253.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa28503
Clone ID Related Accession (Same CDS sequence)	XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
Accession Version	XM_024978485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834253.1
CDS	329..1615
Translation	MSLGVLWKRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVFLLFFSSYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_024978485.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978485.1

ORF Insert Sequence:

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_015475771.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331257.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa28503
Clone ID Related Accession (Same CDS sequence)	XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
Accession Version	XM_015475771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331257.1
CDS	1434..2720
Translation	MSLGVLWKRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVFLLFFSSYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_015475771.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475771.1

ORF Insert Sequence:

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_015475772.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015331258.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa28503
Clone ID Related Accession (Same CDS sequence)	XM_005218714.3 , XM_024978485.1 , XM_015475771.1 , XM_015475772.1
Accession Version	XM_015475772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015331258.1
CDS	835..2121
Translation	MSLGVLWKRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVFLLFFSSYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_015475772.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475772.1

ORF Insert Sequence:

ATGTCTCTGG GAGTTTTGTG GAAGAGGTTT ACCATTGTGG TTGCAACTCA GCTTTCTGAA 
AGTACATTAC TACGTTTAGC AGATGTCCTC TGGAATTCCC AAATCCCTCT TTTGATCTGT 
AGGACATATG GACTAGTTGG TTATATGAGG ATCATTATAA AAGAACATCC AGTAATAGAA 
TCTCATCCAG ATAATGCCTT AGAGGATCTA CGACTGGATA AGCCATTTCC TGAACTGAGA 
GAACATTTTC AGTCTTACGA TTTGGATCAT ATGGAAAAAA AGGACCATAG CCACACTCCA 
TGGATTGTGA TCGTAGCTAA ATACTTAGCA CAGTGGTATA GTGAAACCAA TGGACGAATA 
CCTAAAACAT ATAAAGAAAA AGAAGACTTC AGAGATTTGA TTAGACAAGG AATTTTAAAG 
AATGAAAATG GGACTCCAGA AGATGAAGAG AATTTTGAAG AAGCTATTAA AAATGTGAAT 
ACAGCACTAA ATACAACTCA GATCCCAAGC AGTATTGAAG ATATATTTAA TGATGATCGC 
TGCATAAATA TCACCAAACA GACTCCGACA TTTTGGATTT TAGCTCGTGC CTTAAAGGAA 
TTTGTGGCCA AAGAGGGTCA AGGAAATCTA CCTGTTCGAG GCACAATTCC TGATATGATT 
GCAGATTCAG GCAAATATAT AAAACTTCAA AATGTCTTTT TGTTGTTCTT TTCCAGTTAC 
CGTGAAAAAG CAAAGAAAGA TGCTGCTGCT GTGGGTAATC ATGTTGCCAA ATTGCTTCAG 
TCTATAGGCC AGGCACCAGA GTCCATTTCA GAGAAAGAAT TAAAATTACT CTGCAGCAAT 
TCAGCATTTC TTCGAGTGGT GAGATGTCGA TCCTTAGCTG AAGAATACAG CTTGGATACA 
ATTAACAAGG ATGAAATTAT TTCCAGCATG GACAATCCAG ATAACGAGAT AGTATTATAC 
TTAATGTTAC GGGCTGTTGA TAGATTTCAT AAACAGCATG GTAGATATCC AGGAGTGTCT 
AACTATCAAG TTGAAGAAGA TATAGGAAAG TTGAAGTCTT GTCTCACTAG CTTCCTTCAG 
GAATATGGAT TATCTGTAAT GGTGAAAGAC GATTATGTCC ATGAATTTTG CCGATACGGA 
GCTGCTGAAC CACACACCAT TGCTGCATTC TTAGGGGGAG CTGCTGCTCA AGAGGTTATC 
AAAATAATCA CCAAACAATT TGTAATTTTT AATAATACTT ACATTTATAG TGGCATGTCA 
CAAACTTCAG CAACTTTTCA GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( NM_001193033.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179962.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Price & Availability↑

CloneID	OBa36734
Clone ID Related Accession (Same CDS sequence)	NM_001193033.1
Accession Version	NM_001193033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000018.1. On Jul 17, 2010 this sequence version replaced XM_866024.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571257.29905.1, SRR5571263.18581.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC 
ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA 
GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTTCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT 
GTTGCCAAAT TGCTTCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC AGCATTTCTT CGAGTGGTGA GATGTCGATC CTTAGCTGAA 
GAATACAGCT TGGATACAAT TAACAAGGAT GAAATTATTT CCAGCATGGA CAATCCAGAT 
AACGAGATAG TATTATACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAGCATGGT 
AGATATCCAG GAGTGTCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTAGCT TCCTTCAGGA ATATGGATTA TCTGTAATGG TGAAAGACGA TTATGTCCAT 
GAATTTTGCC GATACGGAGC TGCTGAACCA CACACCATTG CTGCATTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179962.1
CDS	58..1662
Translation	MAQPGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAQAAMEFLQELNNDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	NM_001193033.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193033.1

ORF Insert Sequence:

ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC 
ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA 
GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTTCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT 
GTTGCCAAAT TGCTTCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC AGCATTTCTT CGAGTGGTGA GATGTCGATC CTTAGCTGAA 
GAATACAGCT TGGATACAAT TAACAAGGAT GAAATTATTT CCAGCATGGA CAATCCAGAT 
AACGAGATAG TATTATACTT AATGTTACGG GCTGTTGATA GATTTCATAA ACAGCATGGT 
AGATATCCAG GAGTGTCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTAGCT TCCTTCAGGA ATATGGATTA TCTGTAATGG TGAAAGACGA TTATGTCCAT 
GAATTTTGCC GATACGGAGC TGCTGAACCA CACACCATTG CTGCATTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_005218713.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218770.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28502
Clone ID Related Accession (Same CDS sequence)	XM_005218713.3 , XM_005218713.4
Accession Version	XM_005218713.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104464.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005218713.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC 
ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA 
GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT 
GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG 
TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG 
AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT 
TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT 
AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT 
ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG 
GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT 
GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT 
GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218770.1
CDS	74..1699
Translation	MAQPGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAQAAMEFLQELNNDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVFLLFFSSYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_005218713.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218713.3

ORF Insert Sequence:

ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC 
ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA 
GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT 
GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG 
TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG 
AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT 
TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT 
AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT 
ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG 
GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT 
GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT 
GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_005218713.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005218770.1 Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28502
Clone ID Related Accession (Same CDS sequence)	XM_005218713.3 , XM_005218713.4
Accession Version	XM_005218713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NEDD8-activating enzyme E1 regulatory subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005218713.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC 
ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA 
GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT 
GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG 
TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG 
AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT 
TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT 
AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT 
ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG 
GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT 
GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT 
GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005218770.1
CDS	106..1731
Translation	MAQPGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAQAAMEFLQELNNDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLSESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIVAKYLAQWYSETNGRIPKTYKEKEDFRDLIRQGILKNENGTPEDEENFEEAIKNVNTALNTTQIPSSIEDIFNDDRCINITKQTPTFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVFLLFFSSYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYSLDTINKDEIISSMDNPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTSFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_005218713.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NEDD8 activating enzyme E1 subunit 1 (NAE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005218713.4

ORF Insert Sequence:

ATGGCGCAGC CGGGGAAGCT GCTTAAGGAG CAGAAGTACG ACCGGCAGCT GAGGTTGTGG 
GGTGATCACG GCCAGGAGGC TCTAGAATCT GCTCATGTTT GCCTAATAAA CGCAACAGCC 
ACAGGAACTG AAATCCTTAA AAATTTGGTG CTACCGGGCA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTTCA GAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTGGT TGCAACTCAG CTTTCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCCTTA 
GAGGATCTAC GACTGGATAA GCCATTTCCT GAACTGAGAG AACATTTTCA GTCTTACGAT 
TTGGATCATA TGGAAAAAAA GGACCATAGC CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TACTTAGCAC AGTGGTATAG TGAAACCAAT GGACGAATAC CTAAAACATA TAAAGAAAAA 
GAAGACTTCA GAGATTTGAT TAGACAAGGA ATTTTAAAGA ATGAAAATGG GACTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAATA CAGCACTAAA TACAACTCAG 
ATCCCAAGCA GTATTGAAGA TATATTTAAT GATGATCGCT GCATAAATAT CACCAAACAG 
ACTCCGACAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATCTAC CTGTTCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATATATA 
AAACTTCAAA ATGTCTTTTT GTTGTTCTTT TCCAGTTACC GTGAAAAAGC AAAGAAAGAT 
GCTGCTGCTG TGGGTAATCA TGTTGCCAAA TTGCTTCAGT CTATAGGCCA GGCACCAGAG 
TCCATTTCAG AGAAAGAATT AAAATTACTC TGCAGCAATT CAGCATTTCT TCGAGTGGTG 
AGATGTCGAT CCTTAGCTGA AGAATACAGC TTGGATACAA TTAACAAGGA TGAAATTATT 
TCCAGCATGG ACAATCCAGA TAACGAGATA GTATTATACT TAATGTTACG GGCTGTTGAT 
AGATTTCATA AACAGCATGG TAGATATCCA GGAGTGTCTA ACTATCAAGT TGAAGAAGAT 
ATAGGAAAGT TGAAGTCTTG TCTCACTAGC TTCCTTCAGG AATATGGATT ATCTGTAATG 
GTGAAAGACG ATTATGTCCA TGAATTTTGC CGATACGGAG CTGCTGAACC ACACACCATT 
GCTGCATTCT TAGGGGGAGC TGCTGCTCAA GAGGTTATCA AAATAATCAC CAAACAATTT 
GTAATTTTTA ATAATACTTA CATTTATAGT GGCATGTCAC AAACTTCAGC AACTTTTCAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NAE1
Entrez Gene ID	614491
Full Name	NEDD8 activating enzyme E1 subunit 1
General protein information	Preferred Names NEDD8 activating enzyme E1 subunit 1 Names NEDD8-activating enzyme E1 regulatory subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	NAE1	NP_001233317.1
Gallus gallus (chicken)	NAE1	NP_001006129.1
Arabidopsis thaliana (thale cress)	AXL	NP_180800.1
Arabidopsis thaliana (thale cress)	AXR1	NP_172010.1
Homo sapiens (human)	NAE1	NP_001273429.1
	NAE1	XP_001085059.1
Bos taurus (cattle)	NAE1	NP_001179962.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005544	XP_315544.2
Canis lupus familiaris (dog)	NAE1	XP_003639123.1
Rattus norvegicus (Norway rat)	Nae1	NP_114461.2
Danio rerio (zebrafish)	nae1	NP_956793.1
Drosophila melanogaster (fruit fly)	APP-BP1	NP_648435.1
Oryza sativa (rice)	Os03g0820100	NP_001051717.1
Xenopus tropicalis (tropical clawed frog)	nae1	NP_001120050.1
Mus musculus (house mouse)	Nae1	NP_659180.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NAE1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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