ATP2B2 cDNA ORF clone, Bos taurus(cattle)

The following ATP2B2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP2B2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216330 NM_001191245.2
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa229125 XM_024982976.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229125 XM_024982970.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229125 XM_024982975.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229125 XM_024982971.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229125 XM_024982969.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229125 XM_024982974.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229125 XM_024982972.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229125 XM_024982973.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229126 XM_024982978.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229127 XM_024982979.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229128 XM_024982980.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229129 XM_024982981.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229130 XM_024982982.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229131 XM_024982984.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa229132 XM_024982985.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa39567 NM_001191245.3
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa39567 XM_024982983.1
Latest version!
Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa39567 NM_001191245.1 Bos taurus ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216330
    Clone ID Related Accession (Same CDS sequence) NM_001191245.2
    Accession Version NM_001191245.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02054543.1, DAAA02054539.1, DAAA02054536.1, DAAA02054535.1, DAAA02054534.1, DAAA02054533.1, DAAA02054531.1, DV889527.1, DAAA02054529.1 and DAAA02054528.1. On Aug 9, 2017 this sequence version replaced NM_001191245.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822088, SAMN02822089 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAATGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178174.1
    CDS449..4045
    Translation

    Target ORF information:

    RefSeq Version NM_001191245.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAATGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838738.1
    CDS557..4288
    Translation

    Target ORF information:

    RefSeq Version XM_024982970.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982971.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838739.1
    CDS428..4159
    Translation

    Target ORF information:

    RefSeq Version XM_024982971.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982971.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982976.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838744.1
    CDS381..4112
    Translation

    Target ORF information:

    RefSeq Version XM_024982976.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982975.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838743.1
    CDS417..4148
    Translation

    Target ORF information:

    RefSeq Version XM_024982975.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982975.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838740.1
    CDS342..4073
    Translation

    Target ORF information:

    RefSeq Version XM_024982972.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982973.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838741.1
    CDS490..4221
    Translation

    Target ORF information:

    RefSeq Version XM_024982973.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982973.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982974.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838742.1
    CDS546..4277
    Translation

    Target ORF information:

    RefSeq Version XM_024982974.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982974.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229125
    Clone ID Related Accession (Same CDS sequence) XM_024982970.1 , XM_024982971.1 , XM_024982976.1 , XM_024982975.1 , XM_024982972.1 , XM_024982973.1 , XM_024982974.1 , XM_024982969.1
    Accession Version XM_024982969.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838737.1
    CDS518..4249
    Translation

    Target ORF information:

    RefSeq Version XM_024982969.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229126
    Clone ID Related Accession (Same CDS sequence) XM_024982978.1
    Accession Version XM_024982978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG CCAAACAGCA GGACGGGGCG
    GCCGCCATGG AGATGCAGCC CCTCAAGAGT GCCGAGGGGG GAGATGCTGA TGACAAGAAG
    AAGGCCAACA TGCACAAGAA GGAGAAGTCG GTGCTGCAGG GCAAGCTCAC CAAGCTGGCC
    GTGCAGATCG GCAAGGCGGG CCTGGTGATG TCAGCCATCA CGGTGATCAT CCTGGTGCTC
    TACTTCACCG TGGACACCTT CGTGGTCAAC AAGAAGCCGT GGCTCCCTGA GTGCACACCT
    GTCTACGTGC AGTACTTCGT CAAGTTCTTC ATCATCGGTG TGACGGTGCT GGTGGTCGCC
    GTGCCCGAGG GGCTCCCTCT GGCCGTCACC ATCTCCCTGG CCTACTCGGT GAAGAAAATG
    ATGAAAGACA ACAACCTGGT CCGCCACCTG GACGCCTGCG AGACCATGGG CAACGCCACG
    GCCATCTGCT CGGACAAGAC AGGCACCCTC ACCACCAATC GCATGACGGT GGTGCAGGCC
    TACGTGGGCG ACATCCACTA CAAGGAGATC CCCGACCCCA GCTCCATCAA TGCCAAGACC
    ATGGAGCTGC TGGTCCATGC CATCGCCATC AACAGCGCCT ACACCACCAA GATTCTGCCC
    CCGGAGAAGG AGGGCGCCCT GCCCCGGCAG GTGGGCAACA AGACGGAGTG CGGCCTGCTG
    GGCTTCGTGC TGGACCTGAA GCAAGACTAC GAGCCCGTGC GCGCTCAGAT GCCCGAGGAA
    AAGCTGTACA AGGTCTACAC CTTCAACTCC GTGCGCAAGT CCATGAGCAC CGTCATCAAG
    CTGCCGGACG AGAGCTTCCG CATGTACAGC AAGGGCGCTT CGGAGATCGT GCTCAAGAAG
    TGCTGCAAAA TCCTCAACGG GGTAGGTGAG CCTCGGGTCT TCCGGCCCCG CGACCGGGAC
    GAGATGGTGA AGAAGGTGAT CGAGCCCATG GCCTGCGACG GGCTCCGCAC CATCTGCGTG
    GCTTACCGCG ACTTCCCCAG CAGCCCTGAG CCTGACTGGG ACAACGAGAA CGACATCCTC
    AATGAGCTCA CCTGCATCTG CGTGGTGGGC ATTGAGGATC CCGTGCGGCC TGAGGTCCCG
    GAAGCCATCC GCAAGTGCCA GCGAGCAGGC ATCACGGTCC GCATGGTCAC CGGTGACAAC
    ATCAACACAG CGCGGGCCAT CGCCATCAAG TGTGGCATCA TCCACCCCGG GGAGGACTTC
    CTGTGCCTTG AGGGCAAGGA GTTCAACCGA AGGATCCGCA ACGAGAAAGG GGAGATTGAG
    CAGGAGCGGA TCGATAAGAT CTGGCCAAAG CTGCGGGTGC TGGCTCGCTC CTCACCCACG
    GACAAGCATA CCCTGGTCAA AGGCATCATC GACAGCACGC ACACGGAGCA GCGGCAGGTG
    GTGGCCGTGA CGGGGGACGG CACCAACGAC GGGCCTGCGC TCAAGAAGGC CGACGTGGGC
    TTCGCCATGG GCATCGCAGG CACAGACGTG GCCAAGGAGG CCTCGGACAT CATCCTGACG
    GACGACAACT TCAGCAGCAT CGTCAAGGCG GTGATGTGGG GCCGCAACGT CTACGACAGC
    ATCTCCAAGT TCCTGCAGTT CCAGCTGACC GTCAACGTGG TGGCCGTGAT CGTGGCCTTC
    ACGGGCGCCT GCATCACGCA GGACTCCCCC CTGAAGGCCG TGCAGATGCT CTGGGTGAAC
    CTCATCATGG ACACGTTCGC CTCCTTGGCG CTGGCCACTG AGCCGCCCAC CGAGACCCTG
    CTCCTGAGGA AGCCGTATGG CCGCAACAAG CCCCTCATCT CTCGGACCAT GATGAAGAAT
    ATCCTGGGCC ATGCCGTCTA CCAGCTCACC CTCATCTTCA CCCTGCTCTT CGTCGGCGAG
    AAGATGTTCC AGATCGACAG CGGGAGGAAC GCACCCCTGC ACTCGCCGCC CTCTGAGCAC
    TATACCATCA TCTTCAACAC CTTCGTCATG ATGCAGCTCT TCAACGAAAT CAATGCCCGC
    AAGATCCACG GCGAGCGCAA TGTTTTCGAC GGCATCTTCC GGAACCCCAT CTTCTGCACC
    ATCGTACTGG GCACCTTTGC CATCCAGATA GTGATCGTGC AGTTTGGGGG GAAGCCCTTC
    AGCTGCTCCC CGCTGCAGCT GGACCAGTGG ATGTGGTGCA TATTCATCGG GTTAGGAGAG
    CTTGTCTGGG GCCAGGTCAT CGCCACCATC CCGACCAGCA GGCTCAAGTT CCTCAAGGAG
    GCGGGCAGGC TCACGCAGAA GGAGGAGATC CCCGAGGAGG AGCTCAACGA GGACGTGGAG
    GAGATAGACC ACGCCGAGCG CGAGCTGCGG CGGGGCCAGA TCCTGTGGTT CCGAGGTCTG
    AACCGGATCC AGACGCAGAT CCGCGTCGTG AAGGCGTTTC GTAGCTCTCT CTATGAAGGT
    CTAGAAAAGC CTGAGTCTCG AACCTCCATC CATAACTTCA TGGCTCATCC TGAGTTCCGG
    ATCGAAGATT CGCAGCCCCA CATCCCCCTC ATCGACGACA CCGACCTGGA AGAAGATGCC
    GCGCTCAAGC AGAACTCGAG CCCGCCCTCG TCGCTCAACA AAAACAACAG CGCCATCGAC
    AGCGGGATCA ACCTGACGAC CGACACAAGC AAATCAGCTA CCTCTTCAAG TCCAGGGAGC
    CCCATCCACA GCCTGGAGAC GTCGCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838746.1
    CDS518..4207
    Translation

    Target ORF information:

    RefSeq Version XM_024982978.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG CCAAACAGCA GGACGGGGCG
    GCCGCCATGG AGATGCAGCC CCTCAAGAGT GCCGAGGGGG GAGATGCTGA TGACAAGAAG
    AAGGCCAACA TGCACAAGAA GGAGAAGTCG GTGCTGCAGG GCAAGCTCAC CAAGCTGGCC
    GTGCAGATCG GCAAGGCGGG CCTGGTGATG TCAGCCATCA CGGTGATCAT CCTGGTGCTC
    TACTTCACCG TGGACACCTT CGTGGTCAAC AAGAAGCCGT GGCTCCCTGA GTGCACACCT
    GTCTACGTGC AGTACTTCGT CAAGTTCTTC ATCATCGGTG TGACGGTGCT GGTGGTCGCC
    GTGCCCGAGG GGCTCCCTCT GGCCGTCACC ATCTCCCTGG CCTACTCGGT GAAGAAAATG
    ATGAAAGACA ACAACCTGGT CCGCCACCTG GACGCCTGCG AGACCATGGG CAACGCCACG
    GCCATCTGCT CGGACAAGAC AGGCACCCTC ACCACCAATC GCATGACGGT GGTGCAGGCC
    TACGTGGGCG ACATCCACTA CAAGGAGATC CCCGACCCCA GCTCCATCAA TGCCAAGACC
    ATGGAGCTGC TGGTCCATGC CATCGCCATC AACAGCGCCT ACACCACCAA GATTCTGCCC
    CCGGAGAAGG AGGGCGCCCT GCCCCGGCAG GTGGGCAACA AGACGGAGTG CGGCCTGCTG
    GGCTTCGTGC TGGACCTGAA GCAAGACTAC GAGCCCGTGC GCGCTCAGAT GCCCGAGGAA
    AAGCTGTACA AGGTCTACAC CTTCAACTCC GTGCGCAAGT CCATGAGCAC CGTCATCAAG
    CTGCCGGACG AGAGCTTCCG CATGTACAGC AAGGGCGCTT CGGAGATCGT GCTCAAGAAG
    TGCTGCAAAA TCCTCAACGG GGTAGGTGAG CCTCGGGTCT TCCGGCCCCG CGACCGGGAC
    GAGATGGTGA AGAAGGTGAT CGAGCCCATG GCCTGCGACG GGCTCCGCAC CATCTGCGTG
    GCTTACCGCG ACTTCCCCAG CAGCCCTGAG CCTGACTGGG ACAACGAGAA CGACATCCTC
    AATGAGCTCA CCTGCATCTG CGTGGTGGGC ATTGAGGATC CCGTGCGGCC TGAGGTCCCG
    GAAGCCATCC GCAAGTGCCA GCGAGCAGGC ATCACGGTCC GCATGGTCAC CGGTGACAAC
    ATCAACACAG CGCGGGCCAT CGCCATCAAG TGTGGCATCA TCCACCCCGG GGAGGACTTC
    CTGTGCCTTG AGGGCAAGGA GTTCAACCGA AGGATCCGCA ACGAGAAAGG GGAGATTGAG
    CAGGAGCGGA TCGATAAGAT CTGGCCAAAG CTGCGGGTGC TGGCTCGCTC CTCACCCACG
    GACAAGCATA CCCTGGTCAA AGGCATCATC GACAGCACGC ACACGGAGCA GCGGCAGGTG
    GTGGCCGTGA CGGGGGACGG CACCAACGAC GGGCCTGCGC TCAAGAAGGC CGACGTGGGC
    TTCGCCATGG GCATCGCAGG CACAGACGTG GCCAAGGAGG CCTCGGACAT CATCCTGACG
    GACGACAACT TCAGCAGCAT CGTCAAGGCG GTGATGTGGG GCCGCAACGT CTACGACAGC
    ATCTCCAAGT TCCTGCAGTT CCAGCTGACC GTCAACGTGG TGGCCGTGAT CGTGGCCTTC
    ACGGGCGCCT GCATCACGCA GGACTCCCCC CTGAAGGCCG TGCAGATGCT CTGGGTGAAC
    CTCATCATGG ACACGTTCGC CTCCTTGGCG CTGGCCACTG AGCCGCCCAC CGAGACCCTG
    CTCCTGAGGA AGCCGTATGG CCGCAACAAG CCCCTCATCT CTCGGACCAT GATGAAGAAT
    ATCCTGGGCC ATGCCGTCTA CCAGCTCACC CTCATCTTCA CCCTGCTCTT CGTCGGCGAG
    AAGATGTTCC AGATCGACAG CGGGAGGAAC GCACCCCTGC ACTCGCCGCC CTCTGAGCAC
    TATACCATCA TCTTCAACAC CTTCGTCATG ATGCAGCTCT TCAACGAAAT CAATGCCCGC
    AAGATCCACG GCGAGCGCAA TGTTTTCGAC GGCATCTTCC GGAACCCCAT CTTCTGCACC
    ATCGTACTGG GCACCTTTGC CATCCAGATA GTGATCGTGC AGTTTGGGGG GAAGCCCTTC
    AGCTGCTCCC CGCTGCAGCT GGACCAGTGG ATGTGGTGCA TATTCATCGG GTTAGGAGAG
    CTTGTCTGGG GCCAGGTCAT CGCCACCATC CCGACCAGCA GGCTCAAGTT CCTCAAGGAG
    GCGGGCAGGC TCACGCAGAA GGAGGAGATC CCCGAGGAGG AGCTCAACGA GGACGTGGAG
    GAGATAGACC ACGCCGAGCG CGAGCTGCGG CGGGGCCAGA TCCTGTGGTT CCGAGGTCTG
    AACCGGATCC AGACGCAGAT CCGCGTCGTG AAGGCGTTTC GTAGCTCTCT CTATGAAGGT
    CTAGAAAAGC CTGAGTCTCG AACCTCCATC CATAACTTCA TGGCTCATCC TGAGTTCCGG
    ATCGAAGATT CGCAGCCCCA CATCCCCCTC ATCGACGACA CCGACCTGGA AGAAGATGCC
    GCGCTCAAGC AGAACTCGAG CCCGCCCTCG TCGCTCAACA AAAACAACAG CGCCATCGAC
    AGCGGGATCA ACCTGACGAC CGACACAAGC AAATCAGCTA CCTCTTCAAG TCCAGGGAGC
    CCCATCCACA GCCTGGAGAC GTCGCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229127
    Clone ID Related Accession (Same CDS sequence) XM_024982979.1
    Accession Version XM_024982979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838747.1
    CDS517..4188
    Translation

    Target ORF information:

    RefSeq Version XM_024982979.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATCCGCGTCG TGAAGGCGTT TCGTAGCTCT CTCTATGAAG GTCTAGAAAA GCCTGAGTCT
    CGAACCTCCA TCCATAACTT CATGGCTCAT CCTGAGTTCC GGATCGAAGA TTCGCAGCCC
    CACATCCCCC TCATCGACGA CACCGACCTG GAAGAAGATG CCGCGCTCAA GCAGAACTCG
    AGCCCGCCCT CGTCGCTCAA CAAAAACAAC AGCGCCATCG ACAGCGGGAT CAACCTGACG
    ACCGACACAA GCAAATCAGC TACCTCTTCA AGTCCAGGGA GCCCCATCCA CAGCCTGGAG
    ACGTCGCTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229128
    Clone ID Related Accession (Same CDS sequence) XM_024982980.1
    Accession Version XM_024982980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTA AAATGCAGGA TGGCAATGCG GACGCCAGCC AGAGCAAAGC CAAACAGCAG
    GACGGGGCGG CCGCCATGGA GATGCAGCCC CTCAAGAGTG CCGAGGGGGG AGATGCTGAT
    GACAAGAAGA AGGCCAACAT GCACAAGAAG GAGAAGTCGG TGCTGCAGGG CAAGCTCACC
    AAGCTGGCCG TGCAGATCGG CAAGGCGGGC CTGGTGATGT CAGCCATCAC GGTGATCATC
    CTGGTGCTCT ACTTCACCGT GGACACCTTC GTGGTCAACA AGAAGCCGTG GCTCCCTGAG
    TGCACACCTG TCTACGTGCA GTACTTCGTC AAGTTCTTCA TCATCGGTGT GACGGTGCTG
    GTGGTCGCCG TGCCCGAGGG GCTCCCTCTG GCCGTCACCA TCTCCCTGGC CTACTCGGTG
    AAGAAAATGA TGAAAGACAA CAACCTGGTC CGCCACCTGG ACGCCTGCGA GACCATGGGC
    AACGCCACGG CCATCTGCTC GGACAAGACA GGCACCCTCA CCACCAATCG CATGACGGTG
    GTGCAGGCCT ACGTGGGCGA CATCCACTAC AAGGAGATCC CCGACCCCAG CTCCATCAAT
    GCCAAGACCA TGGAGCTGCT GGTCCATGCC ATCGCCATCA ACAGCGCCTA CACCACCAAG
    ATTCTGCCCC CGGAGAAGGA GGGCGCCCTG CCCCGGCAGG TGGGCAACAA GACGGAGTGC
    GGCCTGCTGG GCTTCGTGCT GGACCTGAAG CAAGACTACG AGCCCGTGCG CGCTCAGATG
    CCCGAGGAAA AGCTGTACAA GGTCTACACC TTCAACTCCG TGCGCAAGTC CATGAGCACC
    GTCATCAAGC TGCCGGACGA GAGCTTCCGC ATGTACAGCA AGGGCGCTTC GGAGATCGTG
    CTCAAGAAGT GCTGCAAAAT CCTCAACGGG GTAGGTGAGC CTCGGGTCTT CCGGCCCCGC
    GACCGGGACG AGATGGTGAA GAAGGTGATC GAGCCCATGG CCTGCGACGG GCTCCGCACC
    ATCTGCGTGG CTTACCGCGA CTTCCCCAGC AGCCCTGAGC CTGACTGGGA CAACGAGAAC
    GACATCCTCA ATGAGCTCAC CTGCATCTGC GTGGTGGGCA TTGAGGATCC CGTGCGGCCT
    GAGGTCCCGG AAGCCATCCG CAAGTGCCAG CGAGCAGGCA TCACGGTCCG CATGGTCACC
    GGTGACAACA TCAACACAGC GCGGGCCATC GCCATCAAGT GTGGCATCAT CCACCCCGGG
    GAGGACTTCC TGTGCCTTGA GGGCAAGGAG TTCAACCGAA GGATCCGCAA CGAGAAAGGG
    GAGATTGAGC AGGAGCGGAT CGATAAGATC TGGCCAAAGC TGCGGGTGCT GGCTCGCTCC
    TCACCCACGG ACAAGCATAC CCTGGTCAAA GGCATCATCG ACAGCACGCA CACGGAGCAG
    CGGCAGGTGG TGGCCGTGAC GGGGGACGGC ACCAACGACG GGCCTGCGCT CAAGAAGGCC
    GACGTGGGCT TCGCCATGGG CATCGCAGGC ACAGACGTGG CCAAGGAGGC CTCGGACATC
    ATCCTGACGG ACGACAACTT CAGCAGCATC GTCAAGGCGG TGATGTGGGG CCGCAACGTC
    TACGACAGCA TCTCCAAGTT CCTGCAGTTC CAGCTGACCG TCAACGTGGT GGCCGTGATC
    GTGGCCTTCA CGGGCGCCTG CATCACGCAG GACTCCCCCC TGAAGGCCGT GCAGATGCTC
    TGGGTGAACC TCATCATGGA CACGTTCGCC TCCTTGGCGC TGGCCACTGA GCCGCCCACC
    GAGACCCTGC TCCTGAGGAA GCCGTATGGC CGCAACAAGC CCCTCATCTC TCGGACCATG
    ATGAAGAATA TCCTGGGCCA TGCCGTCTAC CAGCTCACCC TCATCTTCAC CCTGCTCTTC
    GTCGGCGAGA AGATGTTCCA GATCGACAGC GGGAGGAACG CACCCCTGCA CTCGCCGCCC
    TCTGAGCACT ATACCATCAT CTTCAACACC TTCGTCATGA TGCAGCTCTT CAACGAAATC
    AATGCCCGCA AGATCCACGG CGAGCGCAAT GTTTTCGACG GCATCTTCCG GAACCCCATC
    TTCTGCACCA TCGTACTGGG CACCTTTGCC ATCCAGATAG TGATCGTGCA GTTTGGGGGG
    AAGCCCTTCA GCTGCTCCCC GCTGCAGCTG GACCAGTGGA TGTGGTGCAT ATTCATCGGG
    TTAGGAGAGC TTGTCTGGGG CCAGGTCATC GCCACCATCC CGACCAGCAG GCTCAAGTTC
    CTCAAGGAGG CGGGCAGGCT CACGCAGAAG GAGGAGATCC CCGAGGAGGA GCTCAACGAG
    GACGTGGAGG AGATAGACCA CGCCGAGCGC GAGCTGCGGC GGGGCCAGAT CCTGTGGTTC
    CGAGGTCTGA ACCGGATCCA GACGCAGATC CGCGTCGTGA AGGCGTTTCG TAGCTCTCTC
    TATGAAGGTC TAGAAAAGCC TGAGTCTCGA ACCTCCATCC ATAACTTCAT GGCTCATCCT
    GAGTTCCGGA TCGAAGATTC GCAGCCCCAC ATCCCCCTCA TCGACGACAC CGACCTGGAA
    GAAGATGCCG CGCTCAAGCA GAACTCGAGC CCGCCCTCGT CGCTCAACAA AAACAACAGC
    GCCATCGACA GCGGGATCAA CCTGACGACC GACACAAGCA AATCAGCTAC CTCTTCAAGT
    CCAGGGAGCC CCATCCACAG CCTGGAGACG TCGCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838748.1
    CDS517..4155
    Translation

    Target ORF information:

    RefSeq Version XM_024982980.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTA AAATGCAGGA TGGCAATGCG GACGCCAGCC AGAGCAAAGC CAAACAGCAG
    GACGGGGCGG CCGCCATGGA GATGCAGCCC CTCAAGAGTG CCGAGGGGGG AGATGCTGAT
    GACAAGAAGA AGGCCAACAT GCACAAGAAG GAGAAGTCGG TGCTGCAGGG CAAGCTCACC
    AAGCTGGCCG TGCAGATCGG CAAGGCGGGC CTGGTGATGT CAGCCATCAC GGTGATCATC
    CTGGTGCTCT ACTTCACCGT GGACACCTTC GTGGTCAACA AGAAGCCGTG GCTCCCTGAG
    TGCACACCTG TCTACGTGCA GTACTTCGTC AAGTTCTTCA TCATCGGTGT GACGGTGCTG
    GTGGTCGCCG TGCCCGAGGG GCTCCCTCTG GCCGTCACCA TCTCCCTGGC CTACTCGGTG
    AAGAAAATGA TGAAAGACAA CAACCTGGTC CGCCACCTGG ACGCCTGCGA GACCATGGGC
    AACGCCACGG CCATCTGCTC GGACAAGACA GGCACCCTCA CCACCAATCG CATGACGGTG
    GTGCAGGCCT ACGTGGGCGA CATCCACTAC AAGGAGATCC CCGACCCCAG CTCCATCAAT
    GCCAAGACCA TGGAGCTGCT GGTCCATGCC ATCGCCATCA ACAGCGCCTA CACCACCAAG
    ATTCTGCCCC CGGAGAAGGA GGGCGCCCTG CCCCGGCAGG TGGGCAACAA GACGGAGTGC
    GGCCTGCTGG GCTTCGTGCT GGACCTGAAG CAAGACTACG AGCCCGTGCG CGCTCAGATG
    CCCGAGGAAA AGCTGTACAA GGTCTACACC TTCAACTCCG TGCGCAAGTC CATGAGCACC
    GTCATCAAGC TGCCGGACGA GAGCTTCCGC ATGTACAGCA AGGGCGCTTC GGAGATCGTG
    CTCAAGAAGT GCTGCAAAAT CCTCAACGGG GTAGGTGAGC CTCGGGTCTT CCGGCCCCGC
    GACCGGGACG AGATGGTGAA GAAGGTGATC GAGCCCATGG CCTGCGACGG GCTCCGCACC
    ATCTGCGTGG CTTACCGCGA CTTCCCCAGC AGCCCTGAGC CTGACTGGGA CAACGAGAAC
    GACATCCTCA ATGAGCTCAC CTGCATCTGC GTGGTGGGCA TTGAGGATCC CGTGCGGCCT
    GAGGTCCCGG AAGCCATCCG CAAGTGCCAG CGAGCAGGCA TCACGGTCCG CATGGTCACC
    GGTGACAACA TCAACACAGC GCGGGCCATC GCCATCAAGT GTGGCATCAT CCACCCCGGG
    GAGGACTTCC TGTGCCTTGA GGGCAAGGAG TTCAACCGAA GGATCCGCAA CGAGAAAGGG
    GAGATTGAGC AGGAGCGGAT CGATAAGATC TGGCCAAAGC TGCGGGTGCT GGCTCGCTCC
    TCACCCACGG ACAAGCATAC CCTGGTCAAA GGCATCATCG ACAGCACGCA CACGGAGCAG
    CGGCAGGTGG TGGCCGTGAC GGGGGACGGC ACCAACGACG GGCCTGCGCT CAAGAAGGCC
    GACGTGGGCT TCGCCATGGG CATCGCAGGC ACAGACGTGG CCAAGGAGGC CTCGGACATC
    ATCCTGACGG ACGACAACTT CAGCAGCATC GTCAAGGCGG TGATGTGGGG CCGCAACGTC
    TACGACAGCA TCTCCAAGTT CCTGCAGTTC CAGCTGACCG TCAACGTGGT GGCCGTGATC
    GTGGCCTTCA CGGGCGCCTG CATCACGCAG GACTCCCCCC TGAAGGCCGT GCAGATGCTC
    TGGGTGAACC TCATCATGGA CACGTTCGCC TCCTTGGCGC TGGCCACTGA GCCGCCCACC
    GAGACCCTGC TCCTGAGGAA GCCGTATGGC CGCAACAAGC CCCTCATCTC TCGGACCATG
    ATGAAGAATA TCCTGGGCCA TGCCGTCTAC CAGCTCACCC TCATCTTCAC CCTGCTCTTC
    GTCGGCGAGA AGATGTTCCA GATCGACAGC GGGAGGAACG CACCCCTGCA CTCGCCGCCC
    TCTGAGCACT ATACCATCAT CTTCAACACC TTCGTCATGA TGCAGCTCTT CAACGAAATC
    AATGCCCGCA AGATCCACGG CGAGCGCAAT GTTTTCGACG GCATCTTCCG GAACCCCATC
    TTCTGCACCA TCGTACTGGG CACCTTTGCC ATCCAGATAG TGATCGTGCA GTTTGGGGGG
    AAGCCCTTCA GCTGCTCCCC GCTGCAGCTG GACCAGTGGA TGTGGTGCAT ATTCATCGGG
    TTAGGAGAGC TTGTCTGGGG CCAGGTCATC GCCACCATCC CGACCAGCAG GCTCAAGTTC
    CTCAAGGAGG CGGGCAGGCT CACGCAGAAG GAGGAGATCC CCGAGGAGGA GCTCAACGAG
    GACGTGGAGG AGATAGACCA CGCCGAGCGC GAGCTGCGGC GGGGCCAGAT CCTGTGGTTC
    CGAGGTCTGA ACCGGATCCA GACGCAGATC CGCGTCGTGA AGGCGTTTCG TAGCTCTCTC
    TATGAAGGTC TAGAAAAGCC TGAGTCTCGA ACCTCCATCC ATAACTTCAT GGCTCATCCT
    GAGTTCCGGA TCGAAGATTC GCAGCCCCAC ATCCCCCTCA TCGACGACAC CGACCTGGAA
    GAAGATGCCG CGCTCAAGCA GAACTCGAGC CCGCCCTCGT CGCTCAACAA AAACAACAGC
    GCCATCGACA GCGGGATCAA CCTGACGACC GACACAAGCA AATCAGCTAC CTCTTCAAGT
    CCAGGGAGCC CCATCCACAG CCTGGAGACG TCGCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229129
    Clone ID Related Accession (Same CDS sequence) XM_024982981.1
    Accession Version XM_024982981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATTGAAGTAG TCAATACTTT CAAGAGCGGA GCCTCCTTTC AGGGGGCCCT GCGGAGACAA
    TCCTCCGTCA CCAGCCAGAG CCAGGACGTA GCCAATCTCT CTAGCCCTAG TCGCGTGTCG
    TTGTCCAATG CTCTTTCCTC TCCGACCAGC CTTCCTCCTG CTGCAGCTGG GCATCCGCGT
    CGTGAAGGCG TTTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838749.1
    CDS518..4135
    Translation

    Target ORF information:

    RefSeq Version XM_024982981.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATTGAAGTAG TCAATACTTT CAAGAGCGGA GCCTCCTTTC AGGGGGCCCT GCGGAGACAA
    TCCTCCGTCA CCAGCCAGAG CCAGGACGTA GCCAATCTCT CTAGCCCTAG TCGCGTGTCG
    TTGTCCAATG CTCTTTCCTC TCCGACCAGC CTTCCTCCTG CTGCAGCTGG GCATCCGCGT
    CGTGAAGGCG TTTCGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229130
    Clone ID Related Accession (Same CDS sequence) XM_024982982.1
    Accession Version XM_024982982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATTGAAGTAG TCAATACTTT CAAGAGCGGA GCCTCCTTTC AGGGGGCCCT GCGGAGACAA
    TCCTCCGTCA CCAGCCAGAG CCAGGACGTA GCCAATCTCT CTAGCCCTAG TCGCGTGTCG
    TTGTCCAATG CTCTTTCCTC TCCGACCAGC CTTCCTCCTG CTGCAGCTGG GCAGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838750.1
    CDS518..4117
    Translation

    Target ORF information:

    RefSeq Version XM_024982982.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTG TGAAGAAGGG GGATGGCCTT CAGCTACCAG CGGCCGATGG TGCGGCAGGT
    TCAAATGCTG CAGATAGTGC AAATACCAGC CTAGTCAATG GTAAAATGCA GGATGGCAAT
    GCGGACGCCA GCCAGAGCAA AGCCAAACAG CAGGACGGGG CGGCCGCCAT GGAGATGCAG
    CCCCTCAAGA GTGCCGAGGG GGGAGATGCT GATGACAAGA AGAAGGCCAA CATGCACAAG
    AAGGAGAAGT CGGTGCTGCA GGGCAAGCTC ACCAAGCTGG CCGTGCAGAT CGGCAAGGCG
    GGCCTGGTGA TGTCAGCCAT CACGGTGATC ATCCTGGTGC TCTACTTCAC CGTGGACACC
    TTCGTGGTCA ACAAGAAGCC GTGGCTCCCT GAGTGCACAC CTGTCTACGT GCAGTACTTC
    GTCAAGTTCT TCATCATCGG TGTGACGGTG CTGGTGGTCG CCGTGCCCGA GGGGCTCCCT
    CTGGCCGTCA CCATCTCCCT GGCCTACTCG GTGAAGAAAA TGATGAAAGA CAACAACCTG
    GTCCGCCACC TGGACGCCTG CGAGACCATG GGCAACGCCA CGGCCATCTG CTCGGACAAG
    ACAGGCACCC TCACCACCAA TCGCATGACG GTGGTGCAGG CCTACGTGGG CGACATCCAC
    TACAAGGAGA TCCCCGACCC CAGCTCCATC AATGCCAAGA CCATGGAGCT GCTGGTCCAT
    GCCATCGCCA TCAACAGCGC CTACACCACC AAGATTCTGC CCCCGGAGAA GGAGGGCGCC
    CTGCCCCGGC AGGTGGGCAA CAAGACGGAG TGCGGCCTGC TGGGCTTCGT GCTGGACCTG
    AAGCAAGACT ACGAGCCCGT GCGCGCTCAG ATGCCCGAGG AAAAGCTGTA CAAGGTCTAC
    ACCTTCAACT CCGTGCGCAA GTCCATGAGC ACCGTCATCA AGCTGCCGGA CGAGAGCTTC
    CGCATGTACA GCAAGGGCGC TTCGGAGATC GTGCTCAAGA AGTGCTGCAA AATCCTCAAC
    GGGGTAGGTG AGCCTCGGGT CTTCCGGCCC CGCGACCGGG ACGAGATGGT GAAGAAGGTG
    ATCGAGCCCA TGGCCTGCGA CGGGCTCCGC ACCATCTGCG TGGCTTACCG CGACTTCCCC
    AGCAGCCCTG AGCCTGACTG GGACAACGAG AACGACATCC TCAATGAGCT CACCTGCATC
    TGCGTGGTGG GCATTGAGGA TCCCGTGCGG CCTGAGGTCC CGGAAGCCAT CCGCAAGTGC
    CAGCGAGCAG GCATCACGGT CCGCATGGTC ACCGGTGACA ACATCAACAC AGCGCGGGCC
    ATCGCCATCA AGTGTGGCAT CATCCACCCC GGGGAGGACT TCCTGTGCCT TGAGGGCAAG
    GAGTTCAACC GAAGGATCCG CAACGAGAAA GGGGAGATTG AGCAGGAGCG GATCGATAAG
    ATCTGGCCAA AGCTGCGGGT GCTGGCTCGC TCCTCACCCA CGGACAAGCA TACCCTGGTC
    AAAGGCATCA TCGACAGCAC GCACACGGAG CAGCGGCAGG TGGTGGCCGT GACGGGGGAC
    GGCACCAACG ACGGGCCTGC GCTCAAGAAG GCCGACGTGG GCTTCGCCAT GGGCATCGCA
    GGCACAGACG TGGCCAAGGA GGCCTCGGAC ATCATCCTGA CGGACGACAA CTTCAGCAGC
    ATCGTCAAGG CGGTGATGTG GGGCCGCAAC GTCTACGACA GCATCTCCAA GTTCCTGCAG
    TTCCAGCTGA CCGTCAACGT GGTGGCCGTG ATCGTGGCCT TCACGGGCGC CTGCATCACG
    CAGGACTCCC CCCTGAAGGC CGTGCAGATG CTCTGGGTGA ACCTCATCAT GGACACGTTC
    GCCTCCTTGG CGCTGGCCAC TGAGCCGCCC ACCGAGACCC TGCTCCTGAG GAAGCCGTAT
    GGCCGCAACA AGCCCCTCAT CTCTCGGACC ATGATGAAGA ATATCCTGGG CCATGCCGTC
    TACCAGCTCA CCCTCATCTT CACCCTGCTC TTCGTCGGCG AGAAGATGTT CCAGATCGAC
    AGCGGGAGGA ACGCACCCCT GCACTCGCCG CCCTCTGAGC ACTATACCAT CATCTTCAAC
    ACCTTCGTCA TGATGCAGCT CTTCAACGAA ATCAATGCCC GCAAGATCCA CGGCGAGCGC
    AATGTTTTCG ACGGCATCTT CCGGAACCCC ATCTTCTGCA CCATCGTACT GGGCACCTTT
    GCCATCCAGA TAGTGATCGT GCAGTTTGGG GGGAAGCCCT TCAGCTGCTC CCCGCTGCAG
    CTGGACCAGT GGATGTGGTG CATATTCATC GGGTTAGGAG AGCTTGTCTG GGGCCAGGTC
    ATCGCCACCA TCCCGACCAG CAGGCTCAAG TTCCTCAAGG AGGCGGGCAG GCTCACGCAG
    AAGGAGGAGA TCCCCGAGGA GGAGCTCAAC GAGGACGTGG AGGAGATAGA CCACGCCGAG
    CGCGAGCTGC GGCGGGGCCA GATCCTGTGG TTCCGAGGTC TGAACCGGAT CCAGACGCAG
    ATTGAAGTAG TCAATACTTT CAAGAGCGGA GCCTCCTTTC AGGGGGCCCT GCGGAGACAA
    TCCTCCGTCA CCAGCCAGAG CCAGGACGTA GCCAATCTCT CTAGCCCTAG TCGCGTGTCG
    TTGTCCAATG CTCTTTCCTC TCCGACCAGC CTTCCTCCTG CTGCAGCTGG GCAGGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229131
    Clone ID Related Accession (Same CDS sequence) XM_024982984.1
    Accession Version XM_024982984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTA AAATGCAGGA TGGCAATGCG GACGCCAGCC AGAGCAAAGC CAAACAGCAG
    GACGGGGCGG CCGCCATGGA GATGCAGCCC CTCAAGAGTG CCGAGGGGGG AGATGCTGAT
    GACAAGAAGA AGGCCAACAT GCACAAGAAG GAGAAGTCGG TGCTGCAGGG CAAGCTCACC
    AAGCTGGCCG TGCAGATCGG CAAGGCGGGC CTGGTGATGT CAGCCATCAC GGTGATCATC
    CTGGTGCTCT ACTTCACCGT GGACACCTTC GTGGTCAACA AGAAGCCGTG GCTCCCTGAG
    TGCACACCTG TCTACGTGCA GTACTTCGTC AAGTTCTTCA TCATCGGTGT GACGGTGCTG
    GTGGTCGCCG TGCCCGAGGG GCTCCCTCTG GCCGTCACCA TCTCCCTGGC CTACTCGGTG
    AAGAAAATGA TGAAAGACAA CAACCTGGTC CGCCACCTGG ACGCCTGCGA GACCATGGGC
    AACGCCACGG CCATCTGCTC GGACAAGACA GGCACCCTCA CCACCAATCG CATGACGGTG
    GTGCAGGCCT ACGTGGGCGA CATCCACTAC AAGGAGATCC CCGACCCCAG CTCCATCAAT
    GCCAAGACCA TGGAGCTGCT GGTCCATGCC ATCGCCATCA ACAGCGCCTA CACCACCAAG
    ATTCTGCCCC CGGAGAAGGA GGGCGCCCTG CCCCGGCAGG TGGGCAACAA GACGGAGTGC
    GGCCTGCTGG GCTTCGTGCT GGACCTGAAG CAAGACTACG AGCCCGTGCG CGCTCAGATG
    CCCGAGGAAA AGCTGTACAA GGTCTACACC TTCAACTCCG TGCGCAAGTC CATGAGCACC
    GTCATCAAGC TGCCGGACGA GAGCTTCCGC ATGTACAGCA AGGGCGCTTC GGAGATCGTG
    CTCAAGAAGT GCTGCAAAAT CCTCAACGGG GTAGGTGAGC CTCGGGTCTT CCGGCCCCGC
    GACCGGGACG AGATGGTGAA GAAGGTGATC GAGCCCATGG CCTGCGACGG GCTCCGCACC
    ATCTGCGTGG CTTACCGCGA CTTCCCCAGC AGCCCTGAGC CTGACTGGGA CAACGAGAAC
    GACATCCTCA ATGAGCTCAC CTGCATCTGC GTGGTGGGCA TTGAGGATCC CGTGCGGCCT
    GAGGTCCCGG AAGCCATCCG CAAGTGCCAG CGAGCAGGCA TCACGGTCCG CATGGTCACC
    GGTGACAACA TCAACACAGC GCGGGCCATC GCCATCAAGT GTGGCATCAT CCACCCCGGG
    GAGGACTTCC TGTGCCTTGA GGGCAAGGAG TTCAACCGAA GGATCCGCAA CGAGAAAGGG
    GAGATTGAGC AGGAGCGGAT CGATAAGATC TGGCCAAAGC TGCGGGTGCT GGCTCGCTCC
    TCACCCACGG ACAAGCATAC CCTGGTCAAA GGCATCATCG ACAGCACGCA CACGGAGCAG
    CGGCAGGTGG TGGCCGTGAC GGGGGACGGC ACCAACGACG GGCCTGCGCT CAAGAAGGCC
    GACGTGGGCT TCGCCATGGG CATCGCAGGC ACAGACGTGG CCAAGGAGGC CTCGGACATC
    ATCCTGACGG ACGACAACTT CAGCAGCATC GTCAAGGCGG TGATGTGGGG CCGCAACGTC
    TACGACAGCA TCTCCAAGTT CCTGCAGTTC CAGCTGACCG TCAACGTGGT GGCCGTGATC
    GTGGCCTTCA CGGGCGCCTG CATCACGCAG GACTCCCCCC TGAAGGCCGT GCAGATGCTC
    TGGGTGAACC TCATCATGGA CACGTTCGCC TCCTTGGCGC TGGCCACTGA GCCGCCCACC
    GAGACCCTGC TCCTGAGGAA GCCGTATGGC CGCAACAAGC CCCTCATCTC TCGGACCATG
    ATGAAGAATA TCCTGGGCCA TGCCGTCTAC CAGCTCACCC TCATCTTCAC CCTGCTCTTC
    GTCGGCGAGA AGATGTTCCA GATCGACAGC GGGAGGAACG CACCCCTGCA CTCGCCGCCC
    TCTGAGCACT ATACCATCAT CTTCAACACC TTCGTCATGA TGCAGCTCTT CAACGAAATC
    AATGCCCGCA AGATCCACGG CGAGCGCAAT GTTTTCGACG GCATCTTCCG GAACCCCATC
    TTCTGCACCA TCGTACTGGG CACCTTTGCC ATCCAGATAG TGATCGTGCA GTTTGGGGGG
    AAGCCCTTCA GCTGCTCCCC GCTGCAGCTG GACCAGTGGA TGTGGTGCAT ATTCATCGGG
    TTAGGAGAGC TTGTCTGGGG CCAGGTCATC GCCACCATCC CGACCAGCAG GCTCAAGTTC
    CTCAAGGAGG CGGGCAGGCT CACGCAGAAG GAGGAGATCC CCGAGGAGGA GCTCAACGAG
    GACGTGGAGG AGATAGACCA CGCCGAGCGC GAGCTGCGGC GGGGCCAGAT CCTGTGGTTC
    CGAGGTCTGA ACCGGATCCA GACGCAGATT GAAGTAGTCA ATACTTTCAA GAGCGGAGCC
    TCCTTTCAGG GGGCCCTGCG GAGACAATCC TCCGTCACCA GCCAGAGCCA GGACGTAGCC
    AATCTCTCTA GCCCTAGTCG CGTGTCGTTG TCCAATGCTC TTTCCTCTCC GACCAGCCTT
    CCTCCTGCTG CAGCTGGGCA TCCGCGTCGT GAAGGCGTTT CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838752.1
    CDS1..3525
    Translation

    Target ORF information:

    RefSeq Version XM_024982984.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGGTA AAATGCAGGA TGGCAATGCG GACGCCAGCC AGAGCAAAGC CAAACAGCAG
    GACGGGGCGG CCGCCATGGA GATGCAGCCC CTCAAGAGTG CCGAGGGGGG AGATGCTGAT
    GACAAGAAGA AGGCCAACAT GCACAAGAAG GAGAAGTCGG TGCTGCAGGG CAAGCTCACC
    AAGCTGGCCG TGCAGATCGG CAAGGCGGGC CTGGTGATGT CAGCCATCAC GGTGATCATC
    CTGGTGCTCT ACTTCACCGT GGACACCTTC GTGGTCAACA AGAAGCCGTG GCTCCCTGAG
    TGCACACCTG TCTACGTGCA GTACTTCGTC AAGTTCTTCA TCATCGGTGT GACGGTGCTG
    GTGGTCGCCG TGCCCGAGGG GCTCCCTCTG GCCGTCACCA TCTCCCTGGC CTACTCGGTG
    AAGAAAATGA TGAAAGACAA CAACCTGGTC CGCCACCTGG ACGCCTGCGA GACCATGGGC
    AACGCCACGG CCATCTGCTC GGACAAGACA GGCACCCTCA CCACCAATCG CATGACGGTG
    GTGCAGGCCT ACGTGGGCGA CATCCACTAC AAGGAGATCC CCGACCCCAG CTCCATCAAT
    GCCAAGACCA TGGAGCTGCT GGTCCATGCC ATCGCCATCA ACAGCGCCTA CACCACCAAG
    ATTCTGCCCC CGGAGAAGGA GGGCGCCCTG CCCCGGCAGG TGGGCAACAA GACGGAGTGC
    GGCCTGCTGG GCTTCGTGCT GGACCTGAAG CAAGACTACG AGCCCGTGCG CGCTCAGATG
    CCCGAGGAAA AGCTGTACAA GGTCTACACC TTCAACTCCG TGCGCAAGTC CATGAGCACC
    GTCATCAAGC TGCCGGACGA GAGCTTCCGC ATGTACAGCA AGGGCGCTTC GGAGATCGTG
    CTCAAGAAGT GCTGCAAAAT CCTCAACGGG GTAGGTGAGC CTCGGGTCTT CCGGCCCCGC
    GACCGGGACG AGATGGTGAA GAAGGTGATC GAGCCCATGG CCTGCGACGG GCTCCGCACC
    ATCTGCGTGG CTTACCGCGA CTTCCCCAGC AGCCCTGAGC CTGACTGGGA CAACGAGAAC
    GACATCCTCA ATGAGCTCAC CTGCATCTGC GTGGTGGGCA TTGAGGATCC CGTGCGGCCT
    GAGGTCCCGG AAGCCATCCG CAAGTGCCAG CGAGCAGGCA TCACGGTCCG CATGGTCACC
    GGTGACAACA TCAACACAGC GCGGGCCATC GCCATCAAGT GTGGCATCAT CCACCCCGGG
    GAGGACTTCC TGTGCCTTGA GGGCAAGGAG TTCAACCGAA GGATCCGCAA CGAGAAAGGG
    GAGATTGAGC AGGAGCGGAT CGATAAGATC TGGCCAAAGC TGCGGGTGCT GGCTCGCTCC
    TCACCCACGG ACAAGCATAC CCTGGTCAAA GGCATCATCG ACAGCACGCA CACGGAGCAG
    CGGCAGGTGG TGGCCGTGAC GGGGGACGGC ACCAACGACG GGCCTGCGCT CAAGAAGGCC
    GACGTGGGCT TCGCCATGGG CATCGCAGGC ACAGACGTGG CCAAGGAGGC CTCGGACATC
    ATCCTGACGG ACGACAACTT CAGCAGCATC GTCAAGGCGG TGATGTGGGG CCGCAACGTC
    TACGACAGCA TCTCCAAGTT CCTGCAGTTC CAGCTGACCG TCAACGTGGT GGCCGTGATC
    GTGGCCTTCA CGGGCGCCTG CATCACGCAG GACTCCCCCC TGAAGGCCGT GCAGATGCTC
    TGGGTGAACC TCATCATGGA CACGTTCGCC TCCTTGGCGC TGGCCACTGA GCCGCCCACC
    GAGACCCTGC TCCTGAGGAA GCCGTATGGC CGCAACAAGC CCCTCATCTC TCGGACCATG
    ATGAAGAATA TCCTGGGCCA TGCCGTCTAC CAGCTCACCC TCATCTTCAC CCTGCTCTTC
    GTCGGCGAGA AGATGTTCCA GATCGACAGC GGGAGGAACG CACCCCTGCA CTCGCCGCCC
    TCTGAGCACT ATACCATCAT CTTCAACACC TTCGTCATGA TGCAGCTCTT CAACGAAATC
    AATGCCCGCA AGATCCACGG CGAGCGCAAT GTTTTCGACG GCATCTTCCG GAACCCCATC
    TTCTGCACCA TCGTACTGGG CACCTTTGCC ATCCAGATAG TGATCGTGCA GTTTGGGGGG
    AAGCCCTTCA GCTGCTCCCC GCTGCAGCTG GACCAGTGGA TGTGGTGCAT ATTCATCGGG
    TTAGGAGAGC TTGTCTGGGG CCAGGTCATC GCCACCATCC CGACCAGCAG GCTCAAGTTC
    CTCAAGGAGG CGGGCAGGCT CACGCAGAAG GAGGAGATCC CCGAGGAGGA GCTCAACGAG
    GACGTGGAGG AGATAGACCA CGCCGAGCGC GAGCTGCGGC GGGGCCAGAT CCTGTGGTTC
    CGAGGTCTGA ACCGGATCCA GACGCAGATT GAAGTAGTCA ATACTTTCAA GAGCGGAGCC
    TCCTTTCAGG GGGCCCTGCG GAGACAATCC TCCGTCACCA GCCAGAGCCA GGACGTAGCC
    AATCTCTCTA GCCCTAGTCG CGTGTCGTTG TCCAATGCTC TTTCCTCTCC GACCAGCCTT
    CCTCCTGCTG CAGCTGGGCA TCCGCGTCGT GAAGGCGTTT CGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229132
    Clone ID Related Accession (Same CDS sequence) XM_024982985.1
    Accession Version XM_024982985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATTGA AGTAGTCAAT
    ACTTTCAAGA GCGGAGCCTC CTTTCAGGGG GCCCTGCGGA GACAATCCTC CGTCACCAGC
    CAGAGCCAGG ACGTAGCCAA TCTCTCTAGC CCTAGTCGCG TGTCGTTGTC CAATGCTCTT
    TCCTCTCCGA CCAGCCTTCC TCCTGCTGCA GCTGGGCATC CGCGTCGTGA AGGCGTTTCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838753.1
    CDS1..3483
    Translation

    Target ORF information:

    RefSeq Version XM_024982985.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATTGA AGTAGTCAAT
    ACTTTCAAGA GCGGAGCCTC CTTTCAGGGG GCCCTGCGGA GACAATCCTC CGTCACCAGC
    CAGAGCCAGG ACGTAGCCAA TCTCTCTAGC CCTAGTCGCG TGTCGTTGTC CAATGCTCTT
    TCCTCTCCGA CCAGCCTTCC TCCTGCTGCA GCTGGGCATC CGCGTCGTGA AGGCGTTTCG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39567
    Clone ID Related Accession (Same CDS sequence) NM_001191245.1 , XM_024982983.1 , NM_001191245.3
    Accession Version NM_001191245.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product plasma membrane calcium-transporting ATPase 2
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03022015.1, AAFC03022011.1, AAFC03022009.1, AAFC03022012.1, AAFC03022010.1 and AAFC03022013.1. On Jul 17, 2010 this sequence version replaced XM_001254882.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN01093730, SAMN01093734 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178174.1
    CDS1..3597
    Translation

    Target ORF information:

    RefSeq Version NM_001191245.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39567
    Clone ID Related Accession (Same CDS sequence) NM_001191245.1 , XM_024982983.1 , NM_001191245.3
    Accession Version NM_001191245.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000022.1. On Dec 20, 2018 this sequence version replaced NM_001191245.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571254.16420.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178174.1
    CDS449..4045
    Translation

    Target ORF information:

    RefSeq Version NM_001191245.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191245.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39567
    Clone ID Related Accession (Same CDS sequence) NM_001191245.1 , XM_024982983.1 , NM_001191245.3
    Accession Version XM_024982983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product plasma membrane calcium-transporting ATPase 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024838751.1
    CDS409..4005
    Translation

    Target ORF information:

    RefSeq Version XM_024982983.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ATPase plasma membrane Ca2+ transporting 2 (ATP2B2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024982983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGTGACA TGACCAACAG CGACTTTTAC TCCAAAAACC AAAGAAACGA GTCGAGCCAT 
    GGGGGCGAGT TTGGGTGCAC GATGGAGGAG CTGCGCTCCC TCATGGAGCT GCGGGGCACC
    GAGGCCGTGG TCAAGATCAA GGAGACCTAC GGGGACACTG ACGCCATCTG CCGACGCCTC
    AAAACCTCTC CTGTTGAAGG TTTGCCGGGC ACTGCTCCAG ACCTGGAGAA GAGAAAGCAA
    ATTTTTGGGC AAAACTTTAT ACCTCCAAAG AAGCCAAAAA CCTTCCTGCA GCTCGTGTGG
    GAGGCCCTGC AGGACGTGAC GCTGATCATT CTGGAAATCG CCGCCATCAT CTCCCTGGGG
    CTCTCCTTCT ACCACCCGCC CGGCGAGAGC AACGAAGGAT GTGCGACGGC CCAGGGCGGG
    GCAGAGGATG AAGGGGAGGC AGAGGCGGGC TGGATCGAGG GGGCTGCCAT CCTCCTGTCG
    GTCATCTGCG TGGTCCTGGT CACCGCCTTC AATGACTGGA GCAAAGAGAA GCAGTTCCGG
    GGCCTGCAGA GCCGCATTGA GCAGGAGCAG AAGTTCACTG TGGTCCGGGC CGGCCAGGTG
    GTCCAGATCC CTGTGGCTGA GATCGTGGTT GGGGACATCG CCCAGGTAAA ATATGGCGAC
    CTCCTCCCTG CTGACGGCCT CTTCATCCAG GGCAACGACC TCAAGATCGA TGAGAGCTCC
    CTGACGGGGG AGTCTGACCA GGTGCGCAAG TCGGTGGACA AGGACCCCAT GCTGCTGTCA
    GGTACCCACG TGATGGAGGG CTCTGGACGG ATGGTGGTGA CTGCCGTGGG TGTGAACTCT
    CAGACTGGCA TTATCTTTAC CCTGCTGGGG GCCGGTGGTG AAGAGGAAGA GAAGAAAGAC
    AAGAAAGCCA AACAGCAGGA CGGGGCGGCC GCCATGGAGA TGCAGCCCCT CAAGAGTGCC
    GAGGGGGGAG ATGCTGATGA CAAGAAGAAG GCCAACATGC ACAAGAAGGA GAAGTCGGTG
    CTGCAGGGCA AGCTCACCAA GCTGGCCGTG CAGATCGGCA AGGCGGGCCT GGTGATGTCA
    GCCATCACGG TGATCATCCT GGTGCTCTAC TTCACCGTGG ACACCTTCGT GGTCAACAAG
    AAGCCGTGGC TCCCTGAGTG CACACCTGTC TACGTGCAGT ACTTCGTCAA GTTCTTCATC
    ATCGGTGTGA CGGTGCTGGT GGTCGCCGTG CCCGAGGGGC TCCCTCTGGC CGTCACCATC
    TCCCTGGCCT ACTCGGTGAA GAAAATGATG AAAGACAACA ACCTGGTCCG CCACCTGGAC
    GCCTGCGAGA CCATGGGCAA CGCCACGGCC ATCTGCTCGG ACAAGACAGG CACCCTCACC
    ACCAATCGCA TGACGGTGGT GCAGGCCTAC GTGGGCGACA TCCACTACAA GGAGATCCCC
    GACCCCAGCT CCATCAATGC CAAGACCATG GAGCTGCTGG TCCATGCCAT CGCCATCAAC
    AGCGCCTACA CCACCAAGAT TCTGCCCCCG GAGAAGGAGG GCGCCCTGCC CCGGCAGGTG
    GGCAACAAGA CGGAGTGCGG CCTGCTGGGC TTCGTGCTGG ACCTGAAGCA AGACTACGAG
    CCCGTGCGCG CTCAGATGCC CGAGGAAAAG CTGTACAAGG TCTACACCTT CAACTCCGTG
    CGCAAGTCCA TGAGCACCGT CATCAAGCTG CCGGACGAGA GCTTCCGCAT GTACAGCAAG
    GGCGCTTCGG AGATCGTGCT CAAGAAGTGC TGCAAAATCC TCAACGGGGT AGGTGAGCCT
    CGGGTCTTCC GGCCCCGCGA CCGGGACGAG ATGGTGAAGA AGGTGATCGA GCCCATGGCC
    TGCGACGGGC TCCGCACCAT CTGCGTGGCT TACCGCGACT TCCCCAGCAG CCCTGAGCCT
    GACTGGGACA ACGAGAACGA CATCCTCAAT GAGCTCACCT GCATCTGCGT GGTGGGCATT
    GAGGATCCCG TGCGGCCTGA GGTCCCGGAA GCCATCCGCA AGTGCCAGCG AGCAGGCATC
    ACGGTCCGCA TGGTCACCGG TGACAACATC AACACAGCGC GGGCCATCGC CATCAAGTGT
    GGCATCATCC ACCCCGGGGA GGACTTCCTG TGCCTTGAGG GCAAGGAGTT CAACCGAAGG
    ATCCGCAACG AGAAAGGGGA GATTGAGCAG GAGCGGATCG ATAAGATCTG GCCAAAGCTG
    CGGGTGCTGG CTCGCTCCTC ACCCACGGAC AAGCATACCC TGGTCAAAGG CATCATCGAC
    AGCACGCACA CGGAGCAGCG GCAGGTGGTG GCCGTGACGG GGGACGGCAC CAACGACGGG
    CCTGCGCTCA AGAAGGCCGA CGTGGGCTTC GCCATGGGCA TCGCAGGCAC AGACGTGGCC
    AAGGAGGCCT CGGACATCAT CCTGACGGAC GACAACTTCA GCAGCATCGT CAAGGCGGTG
    ATGTGGGGCC GCAACGTCTA CGACAGCATC TCCAAGTTCC TGCAGTTCCA GCTGACCGTC
    AACGTGGTGG CCGTGATCGT GGCCTTCACG GGCGCCTGCA TCACGCAGGA CTCCCCCCTG
    AAGGCCGTGC AGATGCTCTG GGTGAACCTC ATCATGGACA CGTTCGCCTC CTTGGCGCTG
    GCCACTGAGC CGCCCACCGA GACCCTGCTC CTGAGGAAGC CGTATGGCCG CAACAAGCCC
    CTCATCTCTC GGACCATGAT GAAGAATATC CTGGGCCATG CCGTCTACCA GCTCACCCTC
    ATCTTCACCC TGCTCTTCGT CGGCGAGAAG ATGTTCCAGA TCGACAGCGG GAGGAACGCA
    CCCCTGCACT CGCCGCCCTC TGAGCACTAT ACCATCATCT TCAACACCTT CGTCATGATG
    CAGCTCTTCA ACGAAATCAA TGCCCGCAAG ATCCACGGCG AGCGCAATGT TTTCGACGGC
    ATCTTCCGGA ACCCCATCTT CTGCACCATC GTACTGGGCA CCTTTGCCAT CCAGATAGTG
    ATCGTGCAGT TTGGGGGGAA GCCCTTCAGC TGCTCCCCGC TGCAGCTGGA CCAGTGGATG
    TGGTGCATAT TCATCGGGTT AGGAGAGCTT GTCTGGGGCC AGGTCATCGC CACCATCCCG
    ACCAGCAGGC TCAAGTTCCT CAAGGAGGCG GGCAGGCTCA CGCAGAAGGA GGAGATCCCC
    GAGGAGGAGC TCAACGAGGA CGTGGAGGAG ATAGACCACG CCGAGCGCGA GCTGCGGCGG
    GGCCAGATCC TGTGGTTCCG AGGTCTGAAC CGGATCCAGA CGCAGATCCG CGTCGTGAAG
    GCGTTTCGTA GCTCTCTCTA TGAAGGTCTA GAAAAGCCTG AGTCTCGAAC CTCCATCCAT
    AACTTCATGG CTCATCCTGA GTTCCGGATC GAAGATTCGC AGCCCCACAT CCCCCTCATC
    GACGACACCG ACCTGGAAGA AGATGCCGCG CTCAAGCAGA ACTCGAGCCC GCCCTCGTCG
    CTCAACAAAA ACAACAGCGC CATCGACAGC GGGATCAACC TGACGACCGA CACAAGCAAA
    TCAGCTACCT CTTCAAGTCC AGGGAGCCCC ATCCACAGCC TGGAGACGTC GCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.