COBL cDNA ORF clone, Bos taurus(cattle)

The following COBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa233887 XM_024990960.1
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa89664 XM_015467932.1
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa89664 XM_015462913.1
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa89665 XM_015467934.1
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa89665 XM_015462914.1
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa89666 XM_015462915.1
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa89666 XM_015467941.1
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa55150 XM_010803966.2
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa55150 XM_010827200.2
Latest version!
Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa233887
    Clone ID Related Accession (Same CDS sequence) XM_024990960.1
    Accession Version XM_024990960.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein cordon-bleu
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGAAACC TTTAGAAAAT CTTCACTCAG CAACGATGAG
    ACAGACAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAAT
    AAGGGCTGCT TGACGACCCC GAACTCCCCG TGTGTGAATC CACGTTCTGT TATCCTGGGT
    CCATCCCTCT CTCTTGGCAA CATCTCAGGG GTGTCGGTCA AGTCGGACCT GAAGAAGCGC
    CGGGCACCCC CTCCTCCAAC CCTGCCCCGC GCAGGGCCAC CTGTGCAAGA CAAGACATCA
    GAAAAGATGT CTCTGGGGTC GCAGATTGAC TTACAGAAGA AGAAGAGGCG GGCACCAGCT
    CCCCCTCCAC CACAGCCCCC ACCACCCAGC CCGCTGGTGC CCCCCCGCAC GGAGGACAGG
    GAGGAGGGCA GGAAGGGCAG GACGGTGAGC CTGTCCATGG GGCCCGGCGG TCCCTGCAGT
    GTGGACGGGG CCCCCCCAGT GCCATCGGAG GCTGAGGAGA CCGTGTCTGT CAGCAGCTGC
    TTTGCCTCAG AGGACACCAC AGAGGACTCA GGTGTGATGA GCTCCCCCTC GGACATCGTG
    TCCCTGGATT CCCAGCACGA CAGCATGAAA TCCAAAGACA GGTGGAGCAC AGACCGGGAG
    GACTGCAGCG ACCAGGACCT GGCTGGGACG CCAGAGCCGG GCCCCCCGAA GAGCCCCTTG
    TGGGAGAGGA GCAGCTTTGA GAACCGGCAT CTGAGAAACA GAAAAGCTGC CCCAGCCTCA
    CACGAGGATG AGGAAATATT CATGACTGGA CAGTTCCAGA AAACCCTTGC AGAACTGGAT
    GAAGACCTGG AAGAAATGGA AGAAAGCTAC GAAGCCGACA CAGGCCCCCT CGCCAACTCC
    ATACATGGCA TGTCCACCCC CCATGGTGAC CCTGAGGCAA TCCCGGTGAC GTTCATAGGG
    GAGGTTCTGG ACGATCCCGT GGACTCAGGC GTGTTTTCCA ATAGAAACAA CAACGCCGGC
    TCCTTCGACT TGGGGAGCAC AGCCGACAGT AAGGCTCAGC CACCACCTCA CCAGGCCGAG
    TGCCCCCAGC AGCACAGCCA GAGGCGGGCA GCAGGGCCCA GCTCACCAGC TCCTTCCCGG
    GAGCCTGAGA GAGAGGTCAG GTCAGCCTTG ACCAACACCT GGAAGGAGGT GGATCCCAGG
    AAGATGGAGC CCACGGCGAG TTCTGCTTCA GCGCTTCACA CTCACCACTG GAATGGGAAG
    GAGGAAGGCA GGGCACCTGT TTCTGCTCAC AGCGGGGGGA CCCCAGCCCC TGGAAGGGTG
    ATCCCACAGC TGGGGAAGGA AAAGGCAAGC GATGGTAAAG GTGATATCTC AACACTACCG
    CCATGGCATC AACGAGGCCA ACACCCAGCG GGGAGTTATG GCCTTAAATA TGGCCTCACA
    ACTTATAAAA TTGTCCCTCC GAAGTCAGAG ATGAAATGCT ATGACCGGGG GGCATCTCTC
    TCCGTGGGTG CCATTAAGAT CGATGAGCTG GGGAACCTGG TGAGCCCCCA CGTGCATACC
    GGCAGGACTG TAGTCCCCTC ATCGCCCACT CTGGAGGCAG AAACCCCACC CATTGGAAAA
    GTCAAAGAAT TCTGGAGATC CAATTCTATA GAGAAGCACT CTGGCCGGCC CATGGAGAGG
    CCCAAGAGGA CCCCCACCCC CACCACCCCC ACGAATCTGC AACCTCAGGA AAGCAGACTG
    AGAGGCGAGC CCACCTCCCC AGACCCCCAG GGAACACTGC CCGGGCCCCA GTCCCCACAC
    TCGGAAGATG GGAGAGCTGT GGGGGAGGGG AGAAGCTGGC CACCCCCAGC AGCCACTCGC
    CCCCTCCAAG TGCCAGCAGC TAACCCCGCG GAAGTCCCGT TTCTCAAACC TCAGAGGAGA
    ACATCCAGTC AGTACATGGC CTCTGCCATT GCCAAGCGGA TAGGGACCCC AAAAGTCCAC
    AGTGATGCGG GGAGGAGACA GGATAACACC CAGAGGACGT ATGAAGGCAC AGCACCAGAG
    CCAGTGGTAG CACCTCCTGT GGTGAAGGAC AGTACCACCC CCAGCCCAAA CCCAGGGACG
    GGCATTCGTC GTGAGGGGGA GGAATCGACG GCAGGACCCC ATCCTGGATG GCAAGTCAGC
    AGCCCTTACA GGAAGCTGTC TGCTCAAGAC TACCGGTCTG GCATCCACAG AAATTCCCAT
    GGCTCACTAG TCACAGCTGC ACAACGGGAC CAGGCTTCTG TGGGACAGAG CAGTGGTCTC
    AGTGGAGAGC AAAACATAAG GACACACAGG ACCGACTCAG CATCCGACCC CAAGTACAAG
    TCTGACGGCC TGAGTCCCTC ACGCCTTCAC TCAGGAGATG ATCAGACTTC AGGCAGCACC
    CTGGTGAATG GCTCCCGGTG GGTCCCCATC CACAGCGAGC CTCCTTGCTC TCCAAGAGTG
    TCAGATACAA ATGGCCGGGC TGGACAGGAG CCCCCACAAC GGGAGGAAAA GCCCAGTTTG
    TTAAGCACAG GTGCACTTGA AGCTGATGGT ACACTGCCAG CCAGCATCTT CGGGCCAAAG
    AAGAAATTCA GGCCTGTGGT CCAGAGGCCG GCTCCGAAAG ACACATCCCT GCACAGCGCC
    TTGATGGAGG CCATCCACTC AGCAGGAGGC AAGGACAGGC TCCGCAAGAT TCCAGAACCC
    GGCTCAAAGG GAGGGCCAAA GAAACCATCC TACACTGAGG CAGACGGCGA GCGCTCAGCC
    CTCCTGGCGG CCATACGTGG CCACAGCGGG ACCTGCAGCC TGAGGAAGGT CACGTCCTCT
    GCCTCTGAGG AGCTCCAGAG CCTCCGCGAT GCCGCGCTGT CTGCACATGG GCCAGACCCC
    TCGCGGGTAG AAGACCTTGG GGTCCAGTCC CAGCCGCCCC CGCCCCCACC CACTCCGGTT
    ACCCAGGCGC CCACCTCATC CTGGTCAGCG TCGAGGTCCA GCGAGGGCCC CCTCGGCAAC
    CCGCTGGACG CCAGACAGGC CTTGATGGAC GCCATCCGCT CCGGCACCGG AGCCGCAAGG
    CTGAGAAAGC ATTCCTGTCC GATCCTCGCC AAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846728.1
    CDS168..3926
    Translation

    Target ORF information:

    RefSeq Version XM_024990960.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024990960.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGAAACC TTTAGAAAAT CTTCACTCAG CAACGATGAG
    ACAGACAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAAT
    AAGGGCTGCT TGACGACCCC GAACTCCCCG TGTGTGAATC CACGTTCTGT TATCCTGGGT
    CCATCCCTCT CTCTTGGCAA CATCTCAGGG GTGTCGGTCA AGTCGGACCT GAAGAAGCGC
    CGGGCACCCC CTCCTCCAAC CCTGCCCCGC GCAGGGCCAC CTGTGCAAGA CAAGACATCA
    GAAAAGATGT CTCTGGGGTC GCAGATTGAC TTACAGAAGA AGAAGAGGCG GGCACCAGCT
    CCCCCTCCAC CACAGCCCCC ACCACCCAGC CCGCTGGTGC CCCCCCGCAC GGAGGACAGG
    GAGGAGGGCA GGAAGGGCAG GACGGTGAGC CTGTCCATGG GGCCCGGCGG TCCCTGCAGT
    GTGGACGGGG CCCCCCCAGT GCCATCGGAG GCTGAGGAGA CCGTGTCTGT CAGCAGCTGC
    TTTGCCTCAG AGGACACCAC AGAGGACTCA GGTGTGATGA GCTCCCCCTC GGACATCGTG
    TCCCTGGATT CCCAGCACGA CAGCATGAAA TCCAAAGACA GGTGGAGCAC AGACCGGGAG
    GACTGCAGCG ACCAGGACCT GGCTGGGACG CCAGAGCCGG GCCCCCCGAA GAGCCCCTTG
    TGGGAGAGGA GCAGCTTTGA GAACCGGCAT CTGAGAAACA GAAAAGCTGC CCCAGCCTCA
    CACGAGGATG AGGAAATATT CATGACTGGA CAGTTCCAGA AAACCCTTGC AGAACTGGAT
    GAAGACCTGG AAGAAATGGA AGAAAGCTAC GAAGCCGACA CAGGCCCCCT CGCCAACTCC
    ATACATGGCA TGTCCACCCC CCATGGTGAC CCTGAGGCAA TCCCGGTGAC GTTCATAGGG
    GAGGTTCTGG ACGATCCCGT GGACTCAGGC GTGTTTTCCA ATAGAAACAA CAACGCCGGC
    TCCTTCGACT TGGGGAGCAC AGCCGACAGT AAGGCTCAGC CACCACCTCA CCAGGCCGAG
    TGCCCCCAGC AGCACAGCCA GAGGCGGGCA GCAGGGCCCA GCTCACCAGC TCCTTCCCGG
    GAGCCTGAGA GAGAGGTCAG GTCAGCCTTG ACCAACACCT GGAAGGAGGT GGATCCCAGG
    AAGATGGAGC CCACGGCGAG TTCTGCTTCA GCGCTTCACA CTCACCACTG GAATGGGAAG
    GAGGAAGGCA GGGCACCTGT TTCTGCTCAC AGCGGGGGGA CCCCAGCCCC TGGAAGGGTG
    ATCCCACAGC TGGGGAAGGA AAAGGCAAGC GATGGTAAAG GTGATATCTC AACACTACCG
    CCATGGCATC AACGAGGCCA ACACCCAGCG GGGAGTTATG GCCTTAAATA TGGCCTCACA
    ACTTATAAAA TTGTCCCTCC GAAGTCAGAG ATGAAATGCT ATGACCGGGG GGCATCTCTC
    TCCGTGGGTG CCATTAAGAT CGATGAGCTG GGGAACCTGG TGAGCCCCCA CGTGCATACC
    GGCAGGACTG TAGTCCCCTC ATCGCCCACT CTGGAGGCAG AAACCCCACC CATTGGAAAA
    GTCAAAGAAT TCTGGAGATC CAATTCTATA GAGAAGCACT CTGGCCGGCC CATGGAGAGG
    CCCAAGAGGA CCCCCACCCC CACCACCCCC ACGAATCTGC AACCTCAGGA AAGCAGACTG
    AGAGGCGAGC CCACCTCCCC AGACCCCCAG GGAACACTGC CCGGGCCCCA GTCCCCACAC
    TCGGAAGATG GGAGAGCTGT GGGGGAGGGG AGAAGCTGGC CACCCCCAGC AGCCACTCGC
    CCCCTCCAAG TGCCAGCAGC TAACCCCGCG GAAGTCCCGT TTCTCAAACC TCAGAGGAGA
    ACATCCAGTC AGTACATGGC CTCTGCCATT GCCAAGCGGA TAGGGACCCC AAAAGTCCAC
    AGTGATGCGG GGAGGAGACA GGATAACACC CAGAGGACGT ATGAAGGCAC AGCACCAGAG
    CCAGTGGTAG CACCTCCTGT GGTGAAGGAC AGTACCACCC CCAGCCCAAA CCCAGGGACG
    GGCATTCGTC GTGAGGGGGA GGAATCGACG GCAGGACCCC ATCCTGGATG GCAAGTCAGC
    AGCCCTTACA GGAAGCTGTC TGCTCAAGAC TACCGGTCTG GCATCCACAG AAATTCCCAT
    GGCTCACTAG TCACAGCTGC ACAACGGGAC CAGGCTTCTG TGGGACAGAG CAGTGGTCTC
    AGTGGAGAGC AAAACATAAG GACACACAGG ACCGACTCAG CATCCGACCC CAAGTACAAG
    TCTGACGGCC TGAGTCCCTC ACGCCTTCAC TCAGGAGATG ATCAGACTTC AGGCAGCACC
    CTGGTGAATG GCTCCCGGTG GGTCCCCATC CACAGCGAGC CTCCTTGCTC TCCAAGAGTG
    TCAGATACAA ATGGCCGGGC TGGACAGGAG CCCCCACAAC GGGAGGAAAA GCCCAGTTTG
    TTAAGCACAG GTGCACTTGA AGCTGATGGT ACACTGCCAG CCAGCATCTT CGGGCCAAAG
    AAGAAATTCA GGCCTGTGGT CCAGAGGCCG GCTCCGAAAG ACACATCCCT GCACAGCGCC
    TTGATGGAGG CCATCCACTC AGCAGGAGGC AAGGACAGGC TCCGCAAGAT TCCAGAACCC
    GGCTCAAAGG GAGGGCCAAA GAAACCATCC TACACTGAGG CAGACGGCGA GCGCTCAGCC
    CTCCTGGCGG CCATACGTGG CCACAGCGGG ACCTGCAGCC TGAGGAAGGT CACGTCCTCT
    GCCTCTGAGG AGCTCCAGAG CCTCCGCGAT GCCGCGCTGT CTGCACATGG GCCAGACCCC
    TCGCGGGTAG AAGACCTTGG GGTCCAGTCC CAGCCGCCCC CGCCCCCACC CACTCCGGTT
    ACCCAGGCGC CCACCTCATC CTGGTCAGCG TCGAGGTCCA GCGAGGGCCC CCTCGGCAAC
    CCGCTGGACG CCAGACAGGC CTTGATGGAC GCCATCCGCT CCGGCACCGG AGCCGCAAGG
    CTGAGAAAGC ATTCCTGTCC GATCCTCGCC AAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89664
    Clone ID Related Accession (Same CDS sequence) XM_015462913.1 , XM_015467932.1
    Accession Version XM_015462913.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644689.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGCATTCC
    TGTCCGATCC TCGCCAAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318399.1
    CDS168..4151
    Translation

    Target ORF information:

    RefSeq Version XM_015462913.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462913.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGCATTCC
    TGTCCGATCC TCGCCAAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89664
    Clone ID Related Accession (Same CDS sequence) XM_015462913.1 , XM_015467932.1
    Accession Version XM_015467932.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103883.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGCATTCC
    TGTCCGATCC TCGCCAAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323418.1
    CDS168..4151
    Translation

    Target ORF information:

    RefSeq Version XM_015467932.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGCATTCC
    TGTCCGATCC TCGCCAAGGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89665
    Clone ID Related Accession (Same CDS sequence) XM_015467934.1 , XM_015462914.1
    Accession Version XM_015467934.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103883.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGGTGCCC
    TTGCTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323420.1
    CDS168..4139
    Translation

    Target ORF information:

    RefSeq Version XM_015467934.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467934.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGGTGCCC
    TTGCTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89665
    Clone ID Related Accession (Same CDS sequence) XM_015467934.1 , XM_015462914.1
    Accession Version XM_015462914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644689.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGGTGCCC
    TTGCTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318400.1
    CDS168..4139
    Translation

    Target ORF information:

    RefSeq Version XM_015462914.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGTTCTT CTGACCAAAA CGAAATCGGA GCCTTCCCTG
    AGCTGTCGAG AAACCTTTAG AAAATCTTCA CTCAGCAACG ATGAGACAGA CAAAGAGAAG
    AAAAAATTTC TGGGATTTTT CAAAGTTAAT AAAAGAAGCA ATAATAAGGG CTGCTTGACG
    ACCCCGAACT CCCCGTGTGT GAATCCACGT TCTGTTATCC TGGGTCCATC CCTCTCTCTT
    GGCAACATCT CAGGGGTGTC GGTCAAGTCG GACCTGAAGA AGCGCCGGGC ACCCCCTCCT
    CCAACCCTGC CCCGCGCAGG GCCACCTGTG CAAGACAAGA CATCAGAAAA GATGTCTCTG
    GGGTCGCAGA TTGACTTACA GAAGAAGAAG AGGCGGGCAC CAGCTCCCCC TCCACCACAG
    CCCCCACCAC CCAGCCCGCT GGTGCCCCCC CGCACGGAGG ACAGGGAGGA GGGCAGGAAG
    GGCAGGACGG GTGTCGGACG GCAGGTGCCA CAGAAGCCCC CCCGAGGCGC CGCTCGGGCC
    CCCCCCCAGC TAGTGCTGCC CCCGCCCCCA CCCTACCCGC CTCCTGCCGC GGACGCGACC
    GAGCCTCCGC TTGGCGCTCC CGGGGAAGGC GCTGCTCCCG AGGCCTCCAG CCCAAGGCCA
    GCCCCCGAAC TGAGCCTGTC CATGGGGCCC GGCGGTCCCT GCAGTGTGGA CGGGGCCCCC
    CCAGTGCCAT CGGAGGCTGA GGAGACCGTG TCTGTCAGCA GCTGCTTTGC CTCAGAGGAC
    ACCACAGAGG ACTCAGGTGT GATGAGCTCC CCCTCGGACA TCGTGTCCCT GGATTCCCAG
    CACGACAGCA TGAAATCCAA AGACAGGTGG AGCACAGACC GGGAGGACTG CAGCGACCAG
    GACCTGGCTG GGACGCCAGA GCCGGGCCCC CCGAAGAGCC CCTTGTGGGA GAGGAGCAGC
    TTTGAGAACC GGCATCTGAG AAACAGAAAA GCTGCCCCAG CCTCACACGA GGATGAGGAA
    ATATTCATGA CTGGACAGTT CCAGAAAACC CTTGCAGAAC TGGATGAAGA CCTGGAAGAA
    ATGGAAGAAA GCTACGAAGC CGACACAGGC CCCCTCGCCA ACTCCATACA TGGCATGTCC
    ACCCCCCATG GTGACCCTGA GGCAATCCCG GTGACGTTCA TAGGGGAGGT TCTGGACGAT
    CCCGTGGACT CAGGCGTGTT TTCCAATAGA AACAACAACG CCGGCTCCTT CGACTTGGGG
    AGCACAGCCG ACAGTAAGGC TCAGCCACCA CCTCACCAGG CCGAGTGCCC CCAGCAGCAC
    AGCCAGAGGC GGGCAGCAGG GCCCAGCTCA CCAGCTCCTT CCCGGGAGCC TGAGAGAGAG
    GTCAGGTCAG CCTTGACCAA CACCTGGAAG GAGGTGGATC CCAGGAAGAT GGAGCCCACG
    GCGAGTTCTG CTTCAGCGCT TCACACTCAC CACTGGAATG GGAAGGAGGA AGGCAGGGCA
    CCTGTTTCTG CTCACAGCGG GGGGACCCCA GCCCCTGGAA GGGTGATCCC ACAGCTGGGG
    AAGGAAAAGG CAAGCGATGG TAAAGGTGAT ATCTCAACAC TACCGCCATG GCATCAACGA
    GGCCAACACC CAGCGGGGAG TTATGGCCTT AAATATGGCC TCACAACTTA TAAAATTGTC
    CCTCCGAAGT CAGAGATGAA ATGCTATGAC CGGGGGGCAT CTCTCTCCGT GGGTGCCATT
    AAGATCGATG AGCTGGGGAA CCTGGTGAGC CCCCACGTGC ATACCGGCAG GACTGTAGTC
    CCCTCATCGC CCACTCTGGA GGCAGAAACC CCACCCATTG GAAAAGTCAA AGAATTCTGG
    AGATCCAATT CTATAGAGAA GCACTCTGGC CGGCCCATGG AGAGGCCCAA GAGGACCCCC
    ACCCCCACCA CCCCCACGAA TCTGCAACCT CAGGAAAGCA GACTGAGAGG CGAGCCCACC
    TCCCCAGACC CCCAGGGAAC ACTGCCCGGG CCCCAGTCCC CACACTCGGA AGATGGGAGA
    GCTGTGGGGG AGGGGAGAAG CTGGCCACCC CCAGCAGCCA CTCGCCCCCT CCAAGTGCCA
    GCAGCTAACC CCGCGGAAGT CCCGTTTCTC AAACCTCAGA GGAGAACATC CAGTCAGTAC
    ATGGCCTCTG CCATTGCCAA GCGGATAGGG ACCCCAAAAG TCCACAGTGA TGCGGGGAGG
    AGACAGGATA ACACCCAGAG GACGTATGAA GGCACAGCAC CAGAGCCAGT GGTAGCACCT
    CCTGTGGTGA AGGACAGTAC CACCCCCAGC CCAAACCCAG GGACGGGCAT TCGTCGTGAG
    GGGGAGGAAT CGACGGCAGG ACCCCATCCT GGATGGCAAG TCAGCAGCCC TTACAGGAAG
    CTGTCTGCTC AAGACTACCG GTCTGGCATC CACAGAAATT CCCATGGCTC ACTAGTCACA
    GCTGCACAAC GGGACCAGGC TTCTGTGGGA CAGAGCAGTG GTCTCAGTGG AGAGCAAAAC
    ATAAGGACAC ACAGGACCGA CTCAGCATCC GACCCCAAGT ACAAGTCTGA CGGCCTGAGT
    CCCTCACGCC TTCACTCAGG AGATGATCAG ACTTCAGGCA GCACCCTGGT GAATGGCTCC
    CGGTGGGTCC CCATCCACAG CGAGCCTCCT TGCTCTCCAA GAGTGTCAGA TACAAATGGC
    CGGGCTGGAC AGGAGCCCCC ACAACGGGAG GAAAAGCCCA GTTTGTTAAG CACAGGTGCA
    CTTGAAGCTG ATGGTACACT GCCAGCCAGC ATCTTCGGGC CAAAGAAGAA ATTCAGGCCT
    GTGGTCCAGA GGCCGGCTCC GAAAGACACA TCCCTGCACA GCGCCTTGAT GGAGGCCATC
    CACTCAGCAG GAGGCAAGGA CAGGCTCCGC AAGATTCCAG AACCCGGCTC AAAGGGAGGG
    CCAAAGAAAC CATCCTACAC TGAGGCAGAC GGCGAGCGCT CAGCCCTCCT GGCGGCCATA
    CGTGGCCACA GCGGGACCTG CAGCCTGAGG AAGGTCACGT CCTCTGCCTC TGAGGAGCTC
    CAGAGCCTCC GCGATGCCGC GCTGTCTGCA CATGGGCCAG ACCCCTCGCG GGTAGAAGAC
    CTTGGGGTCC AGTCCCAGCC GCCCCCGCCC CCACCCACTC CGGTTACCCA GGCGCCCACC
    TCATCCTGGT CAGCGTCGAG GTCCAGCGAG GGCCCCCTCG GCAACCCGCT GGACGCCAGA
    CAGGCCTTGA TGGACGCCAT CCGCTCCGGC ACCGGAGCCG CAAGGCTGAG AAAGGTGCCC
    TTGCTTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89666
    Clone ID Related Accession (Same CDS sequence) XM_015467941.1 , XM_015462915.1
    Accession Version XM_015467941.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103883.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGCTC GCGCTCCCCC GCCTCCTGGG AAGCCTGCCA CCCCCAACTT GCACAGTGGA 
    CAGAGATCTC CTCGCAGAGC ATCCCCCGGG CCCCCACAGA ACCAGCTCAG CAGAAAGCAT
    TCGCTGGACG ACGGGGTGGT GGCCATGACC GTCGTGCTGC CCAGCGGGCT GGAGAAGAGA
    AGTGTGGTCA ACGGGAGCCA TGCGATGATG GACCTACTGG TTGAACTTTG CCTTCAGAAC
    CACCTGAATC CCTCCAACCA TGCCCTGGAG ATCCGGTCTT CAGAAACCCA ACAGCCTTTG
    AATTTTAAGC CAAATACTTT AGTTGGGACC TTGAATGTGC ACACCGTATT TCTGAAAGAA
    AAAGTTCCTG AAGCGAAAGT TAAGCCTGTT CCCCCTAAGG TGCCTGAGAA AACGGTGCGC
    CTAGTGGTGA ATTACCTGCG CACGCAGAAG GCGGTGGTGC GTGTGAGCCC CGAGGTGCCC
    CTGCAGAGCA TCCTCCCGGT CATCTGCGCC AAGTGTGAGG TCAGCCCAGA GCACGTGGTG
    CTCCTCAGGG ACAATGTGGC TGGTGAGGAG CTGGAGCTTT CCAAGTCCCT GAACGAGCTG
    GGGATAAAGG AGTTGTATGC CTGGGACAAC AAGAGAGTTC TTCTGACCAA AACGAAATCG
    GAGCCTTCCC TGAGCTGTCG AGAAACCTTT AGAAAATCTT CACTCAGCAA CGATGAGACA
    GACAAAGAGA AGAAAAAATT TCTGGGATTT TTCAAAGTTA ATAAAAGAAG CAATAATAAG
    GGCTGCTTGA CGACCCCGAA CTCCCCGTGT GTGAATCCAC GTTCTGTTAT CCTGGGTCCA
    TCCCTCTCTC TTGGCAACAT CTCAGGGGTG TCGGTCAAGT CGGACCTGAA GAAGCGCCGG
    GCACCCCCTC CTCCAACCCT GCCCCGCGCA GGGCCACCTG TGCAAGACAA GACATCAGAA
    AAGATGTCTC TGGGGTCGCA GATTGACTTA CAGAAGAAGA AGAGGCGGGC ACCAGCTCCC
    CCTCCACCAC AGCCCCCACC ACCCAGCCCG CTGGTGCCCC CCCGCACGGA GGACAGGGAG
    GAGGGCAGGA AGGGCAGGAC GGGTGTCGGA CGGCAGGTGC CACAGAAGCC CCCCCGAGGC
    GCCGCTCGGG CCCCCCCCCA GCTAGTGCTG CCCCCGCCCC CACCCTACCC GCCTCCTGCC
    GCGGACGCGA CCGAGCCTCC GCTTGGCGCT CCCGGGGAAG GCGCTGCTCC CGAGGCCTCC
    AGCCCAAGGC CAGCCCCCGA ACTGAGCCTG TCCATGGGGC CCGGCGGTCC CTGCAGTGTG
    GACGGGGCCC CCCCAGTGCC ATCGGAGGCT GAGGAGACCG TGTCTGTCAG CAGCTGCTTT
    GCCTCAGAGG ACACCACAGA GGACTCAGGT GTGATGAGCT CCCCCTCGGA CATCGTGTCC
    CTGGATTCCC AGCACGACAG CATGAAATCC AAAGACAGGT GGAGCACAGA CCGGGAGGAC
    TGCAGCGACC AGGACCTGGC TGGGACGCCA GAGCCGGGCC CCCCGAAGAG CCCCTTGTGG
    GAGAGGAGCA GCTTTGAGAA CCGGCATCTG AGAAACAGAA AAGCTGCCCC AGCCTCACAC
    GAGGATGAGG AAATATTCAT GACTGGACAG TTCCAGAAAA CCCTTGCAGA ACTGGATGAA
    GACCTGGAAG AAATGGAAGA AAGCTACGAA GCCGACACAG GCCCCCTCGC CAACTCCATA
    CATGGCATGT CCACCCCCCA TGGTGACCCT GAGGCAATCC CGGTGACGTT CATAGGGGAG
    GTTCTGGACG ATCCCGTGGA CTCAGGCGTG TTTTCCAATA GAAACAACAA CGCCGGCTCC
    TTCGACTTGG GGAGCACAGC CGACAGTAAG GCTCAGCCAC CACCTCACCA GGCCGAGTGC
    CCCCAGCAGC ACAGCCAGAG GCGGGCAGCA GGGCCCAGCT CACCAGCTCC TTCCCGGGAG
    CCTGAGAGAG AGGTCAGGTC AGCCTTGACC AACACCTGGA AGGAGGTGGA TCCCAGGAAG
    ATGGAGCCCA CGGCGAGTTC TGCTTCAGCG CTTCACACTC ACCACTGGAA TGGGAAGGAG
    GAAGGCAGGG CACCTGTTTC TGCTCACAGC GGGGGGACCC CAGCCCCTGG AAGGGTGATC
    CCACAGCTGG GGAAGGAAAA GGCAAGCGAT GGTAAAGGTG ATATCTCAAC ACTACCGCCA
    TGGCATCAAC GAGGCCAACA CCCAGCGGGG AGTTATGGCC TTAAATATGG CCTCACAACT
    TATAAAATTG TCCCTCCGAA GTCAGAGATG AAATGCTATG ACCGGGGGGC ATCTCTCTCC
    GTGGGTGCCA TTAAGATCGA TGAGCTGGGG AACCTGGTGA GCCCCCACGT GCATACCGGC
    AGGACTGTAG TCCCCTCATC GCCCACTCTG GAGGCAGAAA CCCCACCCAT TGGAAAAGTC
    AAAGAATTCT GGAGATCCAA TTCTATAGAG AAGCACTCTG GCCGGCCCAT GGAGAGGCCC
    AAGAGGACCC CCACCCCCAC CACCCCCACG AATCTGCAAC CTCAGGAAAG CAGACTGAGA
    GGCGAGCCCA CCTCCCCAGA CCCCCAGGGA ACACTGCCCG GGCCCCAGTC CCCACACTCG
    GAAGATGGGA GAGCTGTGGG GGAGGGGAGA AGCTGGCCAC CCCCAGCAGC CACTCGCCCC
    CTCCAAGTGC CAGCAGCTAA CCCCGCGGAA GTCCCGTTTC TCAAACCTCA GAGGAGAACA
    TCCAGTCAGT ACATGGCCTC TGCCATTGCC AAGCGGATAG GGACCCCAAA AGTCCACAGT
    GATGCGGGGA GGAGACAGGA TAACACCCAG AGGACGTATG AAGGCACAGC ACCAGAGCCA
    GTGGTAGCAC CTCCTGTGGT GAAGGACAGT ACCACCCCCA GCCCAAACCC AGGGACGGGC
    ATTCGTCGTG AGGGGGAGGA ATCGACGGCA GGACCCCATC CTGGATGGCA AGTCAGCAGC
    CCTTACAGGA AGCTGTCTGC TCAAGACTAC CGGTCTGGCA TCCACAGAAA TTCCCATGGC
    TCACTAGTCA CAGCTGCACA ACGGGACCAG GCTTCTGTGG GACAGAGCAG TGGTCTCAGT
    GGAGAGCAAA ACATAAGGAC ACACAGGACC GACTCAGCAT CCGACCCCAA GTACAAGTCT
    GACGGCCTGA GTCCCTCACG CCTTCACTCA GGAGATGATC AGACTTCAGG CAGCACCCTG
    GTGAATGGCT CCCGGTGGGT CCCCATCCAC AGCGAGCCTC CTTGCTCTCC AAGAGTGTCA
    GATACAAATG GCCGGGCTGG ACAGGAGCCC CCACAACGGG AGGAAAAGCC CAGTTTGTTA
    AGCACAGGTG CACTTGAAGC TGATGGTACA CTGCCAGCCA GCATCTTCGG GCCAAAGAAG
    AAATTCAGGC CTGTGGTCCA GAGGCCGGCT CCGAAAGACA CATCCCTGCA CAGCGCCTTG
    ATGGAGGCCA TCCACTCAGC AGGAGGCAAG GACAGGCTCC GCAAGATTCC AGAACCCGGC
    TCAAAGGGAG GGCCAAAGAA ACCATCCTAC ACTGAGGCAG ACGGCGAGCG CTCAGCCCTC
    CTGGCGGCCA TACGTGGCCA CAGCGGGACC TGCAGCCTGA GGAAGGTCAC GTCCTCTGCC
    TCTGAGGAGC TCCAGAGCCT CCGCGATGCC GCGCTGTCTG CACATGGGCC AGACCCCTCG
    CGGGTAGAAG ACCTTGGGGT CCAGTCCCAG CCGCCCCCGC CCCCACCCAC TCCGGTTACC
    CAGGCGCCCA CCTCATCCTG GTCAGCGTCG AGGTCCAGCG AGGGCCCCCT CGGCAACCCG
    CTGGACGCCA GACAGGCCTT GATGGACGCC ATCCGCTCCG GCACCGGAGC CGCAAGGCTG
    AGAAAGCATT CCTGTCCGAT CCTCGCCAAG GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323427.1
    CDS406..4341
    Translation

    Target ORF information:

    RefSeq Version XM_015467941.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467941.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGCTC GCGCTCCCCC GCCTCCTGGG AAGCCTGCCA CCCCCAACTT GCACAGTGGA 
    CAGAGATCTC CTCGCAGAGC ATCCCCCGGG CCCCCACAGA ACCAGCTCAG CAGAAAGCAT
    TCGCTGGACG ACGGGGTGGT GGCCATGACC GTCGTGCTGC CCAGCGGGCT GGAGAAGAGA
    AGTGTGGTCA ACGGGAGCCA TGCGATGATG GACCTACTGG TTGAACTTTG CCTTCAGAAC
    CACCTGAATC CCTCCAACCA TGCCCTGGAG ATCCGGTCTT CAGAAACCCA ACAGCCTTTG
    AATTTTAAGC CAAATACTTT AGTTGGGACC TTGAATGTGC ACACCGTATT TCTGAAAGAA
    AAAGTTCCTG AAGCGAAAGT TAAGCCTGTT CCCCCTAAGG TGCCTGAGAA AACGGTGCGC
    CTAGTGGTGA ATTACCTGCG CACGCAGAAG GCGGTGGTGC GTGTGAGCCC CGAGGTGCCC
    CTGCAGAGCA TCCTCCCGGT CATCTGCGCC AAGTGTGAGG TCAGCCCAGA GCACGTGGTG
    CTCCTCAGGG ACAATGTGGC TGGTGAGGAG CTGGAGCTTT CCAAGTCCCT GAACGAGCTG
    GGGATAAAGG AGTTGTATGC CTGGGACAAC AAGAGAGTTC TTCTGACCAA AACGAAATCG
    GAGCCTTCCC TGAGCTGTCG AGAAACCTTT AGAAAATCTT CACTCAGCAA CGATGAGACA
    GACAAAGAGA AGAAAAAATT TCTGGGATTT TTCAAAGTTA ATAAAAGAAG CAATAATAAG
    GGCTGCTTGA CGACCCCGAA CTCCCCGTGT GTGAATCCAC GTTCTGTTAT CCTGGGTCCA
    TCCCTCTCTC TTGGCAACAT CTCAGGGGTG TCGGTCAAGT CGGACCTGAA GAAGCGCCGG
    GCACCCCCTC CTCCAACCCT GCCCCGCGCA GGGCCACCTG TGCAAGACAA GACATCAGAA
    AAGATGTCTC TGGGGTCGCA GATTGACTTA CAGAAGAAGA AGAGGCGGGC ACCAGCTCCC
    CCTCCACCAC AGCCCCCACC ACCCAGCCCG CTGGTGCCCC CCCGCACGGA GGACAGGGAG
    GAGGGCAGGA AGGGCAGGAC GGGTGTCGGA CGGCAGGTGC CACAGAAGCC CCCCCGAGGC
    GCCGCTCGGG CCCCCCCCCA GCTAGTGCTG CCCCCGCCCC CACCCTACCC GCCTCCTGCC
    GCGGACGCGA CCGAGCCTCC GCTTGGCGCT CCCGGGGAAG GCGCTGCTCC CGAGGCCTCC
    AGCCCAAGGC CAGCCCCCGA ACTGAGCCTG TCCATGGGGC CCGGCGGTCC CTGCAGTGTG
    GACGGGGCCC CCCCAGTGCC ATCGGAGGCT GAGGAGACCG TGTCTGTCAG CAGCTGCTTT
    GCCTCAGAGG ACACCACAGA GGACTCAGGT GTGATGAGCT CCCCCTCGGA CATCGTGTCC
    CTGGATTCCC AGCACGACAG CATGAAATCC AAAGACAGGT GGAGCACAGA CCGGGAGGAC
    TGCAGCGACC AGGACCTGGC TGGGACGCCA GAGCCGGGCC CCCCGAAGAG CCCCTTGTGG
    GAGAGGAGCA GCTTTGAGAA CCGGCATCTG AGAAACAGAA AAGCTGCCCC AGCCTCACAC
    GAGGATGAGG AAATATTCAT GACTGGACAG TTCCAGAAAA CCCTTGCAGA ACTGGATGAA
    GACCTGGAAG AAATGGAAGA AAGCTACGAA GCCGACACAG GCCCCCTCGC CAACTCCATA
    CATGGCATGT CCACCCCCCA TGGTGACCCT GAGGCAATCC CGGTGACGTT CATAGGGGAG
    GTTCTGGACG ATCCCGTGGA CTCAGGCGTG TTTTCCAATA GAAACAACAA CGCCGGCTCC
    TTCGACTTGG GGAGCACAGC CGACAGTAAG GCTCAGCCAC CACCTCACCA GGCCGAGTGC
    CCCCAGCAGC ACAGCCAGAG GCGGGCAGCA GGGCCCAGCT CACCAGCTCC TTCCCGGGAG
    CCTGAGAGAG AGGTCAGGTC AGCCTTGACC AACACCTGGA AGGAGGTGGA TCCCAGGAAG
    ATGGAGCCCA CGGCGAGTTC TGCTTCAGCG CTTCACACTC ACCACTGGAA TGGGAAGGAG
    GAAGGCAGGG CACCTGTTTC TGCTCACAGC GGGGGGACCC CAGCCCCTGG AAGGGTGATC
    CCACAGCTGG GGAAGGAAAA GGCAAGCGAT GGTAAAGGTG ATATCTCAAC ACTACCGCCA
    TGGCATCAAC GAGGCCAACA CCCAGCGGGG AGTTATGGCC TTAAATATGG CCTCACAACT
    TATAAAATTG TCCCTCCGAA GTCAGAGATG AAATGCTATG ACCGGGGGGC ATCTCTCTCC
    GTGGGTGCCA TTAAGATCGA TGAGCTGGGG AACCTGGTGA GCCCCCACGT GCATACCGGC
    AGGACTGTAG TCCCCTCATC GCCCACTCTG GAGGCAGAAA CCCCACCCAT TGGAAAAGTC
    AAAGAATTCT GGAGATCCAA TTCTATAGAG AAGCACTCTG GCCGGCCCAT GGAGAGGCCC
    AAGAGGACCC CCACCCCCAC CACCCCCACG AATCTGCAAC CTCAGGAAAG CAGACTGAGA
    GGCGAGCCCA CCTCCCCAGA CCCCCAGGGA ACACTGCCCG GGCCCCAGTC CCCACACTCG
    GAAGATGGGA GAGCTGTGGG GGAGGGGAGA AGCTGGCCAC CCCCAGCAGC CACTCGCCCC
    CTCCAAGTGC CAGCAGCTAA CCCCGCGGAA GTCCCGTTTC TCAAACCTCA GAGGAGAACA
    TCCAGTCAGT ACATGGCCTC TGCCATTGCC AAGCGGATAG GGACCCCAAA AGTCCACAGT
    GATGCGGGGA GGAGACAGGA TAACACCCAG AGGACGTATG AAGGCACAGC ACCAGAGCCA
    GTGGTAGCAC CTCCTGTGGT GAAGGACAGT ACCACCCCCA GCCCAAACCC AGGGACGGGC
    ATTCGTCGTG AGGGGGAGGA ATCGACGGCA GGACCCCATC CTGGATGGCA AGTCAGCAGC
    CCTTACAGGA AGCTGTCTGC TCAAGACTAC CGGTCTGGCA TCCACAGAAA TTCCCATGGC
    TCACTAGTCA CAGCTGCACA ACGGGACCAG GCTTCTGTGG GACAGAGCAG TGGTCTCAGT
    GGAGAGCAAA ACATAAGGAC ACACAGGACC GACTCAGCAT CCGACCCCAA GTACAAGTCT
    GACGGCCTGA GTCCCTCACG CCTTCACTCA GGAGATGATC AGACTTCAGG CAGCACCCTG
    GTGAATGGCT CCCGGTGGGT CCCCATCCAC AGCGAGCCTC CTTGCTCTCC AAGAGTGTCA
    GATACAAATG GCCGGGCTGG ACAGGAGCCC CCACAACGGG AGGAAAAGCC CAGTTTGTTA
    AGCACAGGTG CACTTGAAGC TGATGGTACA CTGCCAGCCA GCATCTTCGG GCCAAAGAAG
    AAATTCAGGC CTGTGGTCCA GAGGCCGGCT CCGAAAGACA CATCCCTGCA CAGCGCCTTG
    ATGGAGGCCA TCCACTCAGC AGGAGGCAAG GACAGGCTCC GCAAGATTCC AGAACCCGGC
    TCAAAGGGAG GGCCAAAGAA ACCATCCTAC ACTGAGGCAG ACGGCGAGCG CTCAGCCCTC
    CTGGCGGCCA TACGTGGCCA CAGCGGGACC TGCAGCCTGA GGAAGGTCAC GTCCTCTGCC
    TCTGAGGAGC TCCAGAGCCT CCGCGATGCC GCGCTGTCTG CACATGGGCC AGACCCCTCG
    CGGGTAGAAG ACCTTGGGGT CCAGTCCCAG CCGCCCCCGC CCCCACCCAC TCCGGTTACC
    CAGGCGCCCA CCTCATCCTG GTCAGCGTCG AGGTCCAGCG AGGGCCCCCT CGGCAACCCG
    CTGGACGCCA GACAGGCCTT GATGGACGCC ATCCGCTCCG GCACCGGAGC CGCAAGGCTG
    AGAAAGCATT CCTGTCCGAT CCTCGCCAAG GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89666
    Clone ID Related Accession (Same CDS sequence) XM_015467941.1 , XM_015462915.1
    Accession Version XM_015462915.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644689.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGCTC GCGCTCCCCC GCCTCCTGGG AAGCCTGCCA CCCCCAACTT GCACAGTGGA 
    CAGAGATCTC CTCGCAGAGC ATCCCCCGGG CCCCCACAGA ACCAGCTCAG CAGAAAGCAT
    TCGCTGGACG ACGGGGTGGT GGCCATGACC GTCGTGCTGC CCAGCGGGCT GGAGAAGAGA
    AGTGTGGTCA ACGGGAGCCA TGCGATGATG GACCTACTGG TTGAACTTTG CCTTCAGAAC
    CACCTGAATC CCTCCAACCA TGCCCTGGAG ATCCGGTCTT CAGAAACCCA ACAGCCTTTG
    AATTTTAAGC CAAATACTTT AGTTGGGACC TTGAATGTGC ACACCGTATT TCTGAAAGAA
    AAAGTTCCTG AAGCGAAAGT TAAGCCTGTT CCCCCTAAGG TGCCTGAGAA AACGGTGCGC
    CTAGTGGTGA ATTACCTGCG CACGCAGAAG GCGGTGGTGC GTGTGAGCCC CGAGGTGCCC
    CTGCAGAGCA TCCTCCCGGT CATCTGCGCC AAGTGTGAGG TCAGCCCAGA GCACGTGGTG
    CTCCTCAGGG ACAATGTGGC TGGTGAGGAG CTGGAGCTTT CCAAGTCCCT GAACGAGCTG
    GGGATAAAGG AGTTGTATGC CTGGGACAAC AAGAGAGTTC TTCTGACCAA AACGAAATCG
    GAGCCTTCCC TGAGCTGTCG AGAAACCTTT AGAAAATCTT CACTCAGCAA CGATGAGACA
    GACAAAGAGA AGAAAAAATT TCTGGGATTT TTCAAAGTTA ATAAAAGAAG CAATAATAAG
    GGCTGCTTGA CGACCCCGAA CTCCCCGTGT GTGAATCCAC GTTCTGTTAT CCTGGGTCCA
    TCCCTCTCTC TTGGCAACAT CTCAGGGGTG TCGGTCAAGT CGGACCTGAA GAAGCGCCGG
    GCACCCCCTC CTCCAACCCT GCCCCGCGCA GGGCCACCTG TGCAAGACAA GACATCAGAA
    AAGATGTCTC TGGGGTCGCA GATTGACTTA CAGAAGAAGA AGAGGCGGGC ACCAGCTCCC
    CCTCCACCAC AGCCCCCACC ACCCAGCCCG CTGGTGCCCC CCCGCACGGA GGACAGGGAG
    GAGGGCAGGA AGGGCAGGAC GGGTGTCGGA CGGCAGGTGC CACAGAAGCC CCCCCGAGGC
    GCCGCTCGGG CCCCCCCCCA GCTAGTGCTG CCCCCGCCCC CACCCTACCC GCCTCCTGCC
    GCGGACGCGA CCGAGCCTCC GCTTGGCGCT CCCGGGGAAG GCGCTGCTCC CGAGGCCTCC
    AGCCCAAGGC CAGCCCCCGA ACTGAGCCTG TCCATGGGGC CCGGCGGTCC CTGCAGTGTG
    GACGGGGCCC CCCCAGTGCC ATCGGAGGCT GAGGAGACCG TGTCTGTCAG CAGCTGCTTT
    GCCTCAGAGG ACACCACAGA GGACTCAGGT GTGATGAGCT CCCCCTCGGA CATCGTGTCC
    CTGGATTCCC AGCACGACAG CATGAAATCC AAAGACAGGT GGAGCACAGA CCGGGAGGAC
    TGCAGCGACC AGGACCTGGC TGGGACGCCA GAGCCGGGCC CCCCGAAGAG CCCCTTGTGG
    GAGAGGAGCA GCTTTGAGAA CCGGCATCTG AGAAACAGAA AAGCTGCCCC AGCCTCACAC
    GAGGATGAGG AAATATTCAT GACTGGACAG TTCCAGAAAA CCCTTGCAGA ACTGGATGAA
    GACCTGGAAG AAATGGAAGA AAGCTACGAA GCCGACACAG GCCCCCTCGC CAACTCCATA
    CATGGCATGT CCACCCCCCA TGGTGACCCT GAGGCAATCC CGGTGACGTT CATAGGGGAG
    GTTCTGGACG ATCCCGTGGA CTCAGGCGTG TTTTCCAATA GAAACAACAA CGCCGGCTCC
    TTCGACTTGG GGAGCACAGC CGACAGTAAG GCTCAGCCAC CACCTCACCA GGCCGAGTGC
    CCCCAGCAGC ACAGCCAGAG GCGGGCAGCA GGGCCCAGCT CACCAGCTCC TTCCCGGGAG
    CCTGAGAGAG AGGTCAGGTC AGCCTTGACC AACACCTGGA AGGAGGTGGA TCCCAGGAAG
    ATGGAGCCCA CGGCGAGTTC TGCTTCAGCG CTTCACACTC ACCACTGGAA TGGGAAGGAG
    GAAGGCAGGG CACCTGTTTC TGCTCACAGC GGGGGGACCC CAGCCCCTGG AAGGGTGATC
    CCACAGCTGG GGAAGGAAAA GGCAAGCGAT GGTAAAGGTG ATATCTCAAC ACTACCGCCA
    TGGCATCAAC GAGGCCAACA CCCAGCGGGG AGTTATGGCC TTAAATATGG CCTCACAACT
    TATAAAATTG TCCCTCCGAA GTCAGAGATG AAATGCTATG ACCGGGGGGC ATCTCTCTCC
    GTGGGTGCCA TTAAGATCGA TGAGCTGGGG AACCTGGTGA GCCCCCACGT GCATACCGGC
    AGGACTGTAG TCCCCTCATC GCCCACTCTG GAGGCAGAAA CCCCACCCAT TGGAAAAGTC
    AAAGAATTCT GGAGATCCAA TTCTATAGAG AAGCACTCTG GCCGGCCCAT GGAGAGGCCC
    AAGAGGACCC CCACCCCCAC CACCCCCACG AATCTGCAAC CTCAGGAAAG CAGACTGAGA
    GGCGAGCCCA CCTCCCCAGA CCCCCAGGGA ACACTGCCCG GGCCCCAGTC CCCACACTCG
    GAAGATGGGA GAGCTGTGGG GGAGGGGAGA AGCTGGCCAC CCCCAGCAGC CACTCGCCCC
    CTCCAAGTGC CAGCAGCTAA CCCCGCGGAA GTCCCGTTTC TCAAACCTCA GAGGAGAACA
    TCCAGTCAGT ACATGGCCTC TGCCATTGCC AAGCGGATAG GGACCCCAAA AGTCCACAGT
    GATGCGGGGA GGAGACAGGA TAACACCCAG AGGACGTATG AAGGCACAGC ACCAGAGCCA
    GTGGTAGCAC CTCCTGTGGT GAAGGACAGT ACCACCCCCA GCCCAAACCC AGGGACGGGC
    ATTCGTCGTG AGGGGGAGGA ATCGACGGCA GGACCCCATC CTGGATGGCA AGTCAGCAGC
    CCTTACAGGA AGCTGTCTGC TCAAGACTAC CGGTCTGGCA TCCACAGAAA TTCCCATGGC
    TCACTAGTCA CAGCTGCACA ACGGGACCAG GCTTCTGTGG GACAGAGCAG TGGTCTCAGT
    GGAGAGCAAA ACATAAGGAC ACACAGGACC GACTCAGCAT CCGACCCCAA GTACAAGTCT
    GACGGCCTGA GTCCCTCACG CCTTCACTCA GGAGATGATC AGACTTCAGG CAGCACCCTG
    GTGAATGGCT CCCGGTGGGT CCCCATCCAC AGCGAGCCTC CTTGCTCTCC AAGAGTGTCA
    GATACAAATG GCCGGGCTGG ACAGGAGCCC CCACAACGGG AGGAAAAGCC CAGTTTGTTA
    AGCACAGGTG CACTTGAAGC TGATGGTACA CTGCCAGCCA GCATCTTCGG GCCAAAGAAG
    AAATTCAGGC CTGTGGTCCA GAGGCCGGCT CCGAAAGACA CATCCCTGCA CAGCGCCTTG
    ATGGAGGCCA TCCACTCAGC AGGAGGCAAG GACAGGCTCC GCAAGATTCC AGAACCCGGC
    TCAAAGGGAG GGCCAAAGAA ACCATCCTAC ACTGAGGCAG ACGGCGAGCG CTCAGCCCTC
    CTGGCGGCCA TACGTGGCCA CAGCGGGACC TGCAGCCTGA GGAAGGTCAC GTCCTCTGCC
    TCTGAGGAGC TCCAGAGCCT CCGCGATGCC GCGCTGTCTG CACATGGGCC AGACCCCTCG
    CGGGTAGAAG ACCTTGGGGT CCAGTCCCAG CCGCCCCCGC CCCCACCCAC TCCGGTTACC
    CAGGCGCCCA CCTCATCCTG GTCAGCGTCG AGGTCCAGCG AGGGCCCCCT CGGCAACCCG
    CTGGACGCCA GACAGGCCTT GATGGACGCC ATCCGCTCCG GCACCGGAGC CGCAAGGCTG
    AGAAAGCATT CCTGTCCGAT CCTCGCCAAG GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318401.1
    CDS406..4341
    Translation

    Target ORF information:

    RefSeq Version XM_015462915.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462915.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAAGGCTC GCGCTCCCCC GCCTCCTGGG AAGCCTGCCA CCCCCAACTT GCACAGTGGA 
    CAGAGATCTC CTCGCAGAGC ATCCCCCGGG CCCCCACAGA ACCAGCTCAG CAGAAAGCAT
    TCGCTGGACG ACGGGGTGGT GGCCATGACC GTCGTGCTGC CCAGCGGGCT GGAGAAGAGA
    AGTGTGGTCA ACGGGAGCCA TGCGATGATG GACCTACTGG TTGAACTTTG CCTTCAGAAC
    CACCTGAATC CCTCCAACCA TGCCCTGGAG ATCCGGTCTT CAGAAACCCA ACAGCCTTTG
    AATTTTAAGC CAAATACTTT AGTTGGGACC TTGAATGTGC ACACCGTATT TCTGAAAGAA
    AAAGTTCCTG AAGCGAAAGT TAAGCCTGTT CCCCCTAAGG TGCCTGAGAA AACGGTGCGC
    CTAGTGGTGA ATTACCTGCG CACGCAGAAG GCGGTGGTGC GTGTGAGCCC CGAGGTGCCC
    CTGCAGAGCA TCCTCCCGGT CATCTGCGCC AAGTGTGAGG TCAGCCCAGA GCACGTGGTG
    CTCCTCAGGG ACAATGTGGC TGGTGAGGAG CTGGAGCTTT CCAAGTCCCT GAACGAGCTG
    GGGATAAAGG AGTTGTATGC CTGGGACAAC AAGAGAGTTC TTCTGACCAA AACGAAATCG
    GAGCCTTCCC TGAGCTGTCG AGAAACCTTT AGAAAATCTT CACTCAGCAA CGATGAGACA
    GACAAAGAGA AGAAAAAATT TCTGGGATTT TTCAAAGTTA ATAAAAGAAG CAATAATAAG
    GGCTGCTTGA CGACCCCGAA CTCCCCGTGT GTGAATCCAC GTTCTGTTAT CCTGGGTCCA
    TCCCTCTCTC TTGGCAACAT CTCAGGGGTG TCGGTCAAGT CGGACCTGAA GAAGCGCCGG
    GCACCCCCTC CTCCAACCCT GCCCCGCGCA GGGCCACCTG TGCAAGACAA GACATCAGAA
    AAGATGTCTC TGGGGTCGCA GATTGACTTA CAGAAGAAGA AGAGGCGGGC ACCAGCTCCC
    CCTCCACCAC AGCCCCCACC ACCCAGCCCG CTGGTGCCCC CCCGCACGGA GGACAGGGAG
    GAGGGCAGGA AGGGCAGGAC GGGTGTCGGA CGGCAGGTGC CACAGAAGCC CCCCCGAGGC
    GCCGCTCGGG CCCCCCCCCA GCTAGTGCTG CCCCCGCCCC CACCCTACCC GCCTCCTGCC
    GCGGACGCGA CCGAGCCTCC GCTTGGCGCT CCCGGGGAAG GCGCTGCTCC CGAGGCCTCC
    AGCCCAAGGC CAGCCCCCGA ACTGAGCCTG TCCATGGGGC CCGGCGGTCC CTGCAGTGTG
    GACGGGGCCC CCCCAGTGCC ATCGGAGGCT GAGGAGACCG TGTCTGTCAG CAGCTGCTTT
    GCCTCAGAGG ACACCACAGA GGACTCAGGT GTGATGAGCT CCCCCTCGGA CATCGTGTCC
    CTGGATTCCC AGCACGACAG CATGAAATCC AAAGACAGGT GGAGCACAGA CCGGGAGGAC
    TGCAGCGACC AGGACCTGGC TGGGACGCCA GAGCCGGGCC CCCCGAAGAG CCCCTTGTGG
    GAGAGGAGCA GCTTTGAGAA CCGGCATCTG AGAAACAGAA AAGCTGCCCC AGCCTCACAC
    GAGGATGAGG AAATATTCAT GACTGGACAG TTCCAGAAAA CCCTTGCAGA ACTGGATGAA
    GACCTGGAAG AAATGGAAGA AAGCTACGAA GCCGACACAG GCCCCCTCGC CAACTCCATA
    CATGGCATGT CCACCCCCCA TGGTGACCCT GAGGCAATCC CGGTGACGTT CATAGGGGAG
    GTTCTGGACG ATCCCGTGGA CTCAGGCGTG TTTTCCAATA GAAACAACAA CGCCGGCTCC
    TTCGACTTGG GGAGCACAGC CGACAGTAAG GCTCAGCCAC CACCTCACCA GGCCGAGTGC
    CCCCAGCAGC ACAGCCAGAG GCGGGCAGCA GGGCCCAGCT CACCAGCTCC TTCCCGGGAG
    CCTGAGAGAG AGGTCAGGTC AGCCTTGACC AACACCTGGA AGGAGGTGGA TCCCAGGAAG
    ATGGAGCCCA CGGCGAGTTC TGCTTCAGCG CTTCACACTC ACCACTGGAA TGGGAAGGAG
    GAAGGCAGGG CACCTGTTTC TGCTCACAGC GGGGGGACCC CAGCCCCTGG AAGGGTGATC
    CCACAGCTGG GGAAGGAAAA GGCAAGCGAT GGTAAAGGTG ATATCTCAAC ACTACCGCCA
    TGGCATCAAC GAGGCCAACA CCCAGCGGGG AGTTATGGCC TTAAATATGG CCTCACAACT
    TATAAAATTG TCCCTCCGAA GTCAGAGATG AAATGCTATG ACCGGGGGGC ATCTCTCTCC
    GTGGGTGCCA TTAAGATCGA TGAGCTGGGG AACCTGGTGA GCCCCCACGT GCATACCGGC
    AGGACTGTAG TCCCCTCATC GCCCACTCTG GAGGCAGAAA CCCCACCCAT TGGAAAAGTC
    AAAGAATTCT GGAGATCCAA TTCTATAGAG AAGCACTCTG GCCGGCCCAT GGAGAGGCCC
    AAGAGGACCC CCACCCCCAC CACCCCCACG AATCTGCAAC CTCAGGAAAG CAGACTGAGA
    GGCGAGCCCA CCTCCCCAGA CCCCCAGGGA ACACTGCCCG GGCCCCAGTC CCCACACTCG
    GAAGATGGGA GAGCTGTGGG GGAGGGGAGA AGCTGGCCAC CCCCAGCAGC CACTCGCCCC
    CTCCAAGTGC CAGCAGCTAA CCCCGCGGAA GTCCCGTTTC TCAAACCTCA GAGGAGAACA
    TCCAGTCAGT ACATGGCCTC TGCCATTGCC AAGCGGATAG GGACCCCAAA AGTCCACAGT
    GATGCGGGGA GGAGACAGGA TAACACCCAG AGGACGTATG AAGGCACAGC ACCAGAGCCA
    GTGGTAGCAC CTCCTGTGGT GAAGGACAGT ACCACCCCCA GCCCAAACCC AGGGACGGGC
    ATTCGTCGTG AGGGGGAGGA ATCGACGGCA GGACCCCATC CTGGATGGCA AGTCAGCAGC
    CCTTACAGGA AGCTGTCTGC TCAAGACTAC CGGTCTGGCA TCCACAGAAA TTCCCATGGC
    TCACTAGTCA CAGCTGCACA ACGGGACCAG GCTTCTGTGG GACAGAGCAG TGGTCTCAGT
    GGAGAGCAAA ACATAAGGAC ACACAGGACC GACTCAGCAT CCGACCCCAA GTACAAGTCT
    GACGGCCTGA GTCCCTCACG CCTTCACTCA GGAGATGATC AGACTTCAGG CAGCACCCTG
    GTGAATGGCT CCCGGTGGGT CCCCATCCAC AGCGAGCCTC CTTGCTCTCC AAGAGTGTCA
    GATACAAATG GCCGGGCTGG ACAGGAGCCC CCACAACGGG AGGAAAAGCC CAGTTTGTTA
    AGCACAGGTG CACTTGAAGC TGATGGTACA CTGCCAGCCA GCATCTTCGG GCCAAAGAAG
    AAATTCAGGC CTGTGGTCCA GAGGCCGGCT CCGAAAGACA CATCCCTGCA CAGCGCCTTG
    ATGGAGGCCA TCCACTCAGC AGGAGGCAAG GACAGGCTCC GCAAGATTCC AGAACCCGGC
    TCAAAGGGAG GGCCAAAGAA ACCATCCTAC ACTGAGGCAG ACGGCGAGCG CTCAGCCCTC
    CTGGCGGCCA TACGTGGCCA CAGCGGGACC TGCAGCCTGA GGAAGGTCAC GTCCTCTGCC
    TCTGAGGAGC TCCAGAGCCT CCGCGATGCC GCGCTGTCTG CACATGGGCC AGACCCCTCG
    CGGGTAGAAG ACCTTGGGGT CCAGTCCCAG CCGCCCCCGC CCCCACCCAC TCCGGTTACC
    CAGGCGCCCA CCTCATCCTG GTCAGCGTCG AGGTCCAGCG AGGGCCCCCT CGGCAACCCG
    CTGGACGCCA GACAGGCCTT GATGGACGCC ATCCGCTCCG GCACCGGAGC CGCAAGGCTG
    AGAAAGCATT CCTGTCCGAT CCTCGCCAAG GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55150
    Clone ID Related Accession (Same CDS sequence) XM_010803966.2 , XM_010827200.2
    Accession Version XM_010803966.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103883.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGAAACC TTTAGAAAAT CTTCACTCAG CAACGATGAG
    ACAGACAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAAT
    AAGGGCTGCT TGACGACCCC GAACTCCCCG TGTGTGAATC CACGTTCTGT TATCCTGGGT
    CCATCCCTCT CTCTTGGCAA CATCTCAGGG GTGTCGGTCA AGTCGGACCT GAAGAAGCGC
    CGGGCACCCC CTCCTCCAAC CCTGCCCCGC GCAGGGCCAC CTGTGCAAGA CAAGACATCA
    GAAAAGATGT CTCTGGGGTC GCAGATTGAC TTACAGAAGA AGAAGAGGCG GGCACCAGCT
    CCCCCTCCAC CACAGCCCCC ACCACCCAGC CCGCTGGTGC CCCCCCGCAC GGAGGACAGG
    GAGGAGGGCA GGAAGGGCAG GACGGGTGTC GGACGGCAGG TGCCACAGAA GCCCCCCCGA
    GGCGCCGCTC GGGCCCCCCC CCAGCTAGTG CTGCCCCCGC CCCCACCCTA CCCGCCTCCT
    GCCGCGGACG CGACCGAGCC TCCGCTTGGC GCTCCCGGGG AAGGCGCTGC TCCCGAGGCC
    TCCAGCCCAA GGCCAGCCCC CGAACTGAGC CTGTCCATGG GGCCCGGCGG TCCCTGCAGT
    GTGGACGGGG CCCCCCCAGT GCCATCGGAG GCTGAGGAGA CCGTGTCTGT CAGCAGCTGC
    TTTGCCTCAG AGGACACCAC AGAGGACTCA GGTGTGATGA GCTCCCCCTC GGACATCGTG
    TCCCTGGATT CCCAGCACGA CAGCATGAAA TCCAAAGACA GGTGGAGCAC AGACCGGGAG
    GACTGCAGCG ACCAGGACCT GGCTGGGACG CCAGAGCCGG GCCCCCCGAA GAGCCCCTTG
    TGGGAGAGGA GCAGCTTTGA GAACCGGCAT CTGAGAAACA GAAAAGCTGC CCCAGCCTCA
    CACGAGGATG AGGAAATATT CATGACTGGA CAGTTCCAGA AAACCCTTGC AGAACTGGAT
    GAAGACCTGG AAGAAATGGA AGAAAGCTAC GAAGCCGACA CAGGCCCCCT CGCCAACTCC
    ATACATGGCA TGTCCACCCC CCATGGTGAC CCTGAGGCAA TCCCGGTGAC GTTCATAGGG
    GAGGTTCTGG ACGATCCCGT GGACTCAGGC GTGTTTTCCA ATAGAAACAA CAACGCCGGC
    TCCTTCGACT TGGGGAGCAC AGCCGACAGT AAGGCTCAGC CACCACCTCA CCAGGCCGAG
    TGCCCCCAGC AGCACAGCCA GAGGCGGGCA GCAGGGCCCA GCTCACCAGC TCCTTCCCGG
    GAGCCTGAGA GAGAGGTCAG GTCAGCCTTG ACCAACACCT GGAAGGAGGT GGATCCCAGG
    AAGATGGAGC CCACGGCGAG TTCTGCTTCA GCGCTTCACA CTCACCACTG GAATGGGAAG
    GAGGAAGGCA GGGCACCTGT TTCTGCTCAC AGCGGGGGGA CCCCAGCCCC TGGAAGGGTG
    ATCCCACAGC TGGGGAAGGA AAAGGCAAGC GATGGTAAAG GTGATATCTC AACACTACCG
    CCATGGCATC AACGAGGCCA ACACCCAGCG GGGAGTTATG GCCTTAAATA TGGCCTCACA
    ACTTATAAAA TTGTCCCTCC GAAGTCAGAG ATGAAATGCT ATGACCGGGG GGCATCTCTC
    TCCGTGGGTG CCATTAAGAT CGATGAGCTG GGGAACCTGG TGAGCCCCCA CGTGCATACC
    GGCAGGACTG TAGTCCCCTC ATCGCCCACT CTGGAGGCAG AAACCCCACC CATTGGAAAA
    GTCAAAGAAT TCTGGAGATC CAATTCTATA GAGAAGCACT CTGGCCGGCC CATGGAGAGG
    CCCAAGAGGA CCCCCACCCC CACCACCCCC ACGAATCTGC AACCTCAGGA AAGCAGACTG
    AGAGGCGAGC CCACCTCCCC AGACCCCCAG GGAACACTGC CCGGGCCCCA GTCCCCACAC
    TCGGAAGATG GGAGAGCTGT GGGGGAGGGG AGAAGCTGGC CACCCCCAGC AGCCACTCGC
    CCCCTCCAAG TGCCAGCAGC TAACCCCGCG GAAGTCCCGT TTCTCAAACC TCAGAGGAGA
    ACATCCAGTC AGTACATGGC CTCTGCCATT GCCAAGCGGA TAGGGACCCC AAAAGTCCAC
    AGTGATGCGG GGAGGAGACA GGATAACACC CAGAGGACGT ATGAAGGCAC AGCACCAGAG
    CCAGTGGTAG CACCTCCTGT GGTGAAGGAC AGTACCACCC CCAGCCCAAA CCCAGGGACG
    GGCATTCGTC GTGAGGGGGA GGAATCGACG GCAGGACCCC ATCCTGGATG GCAAGTCAGC
    AGCCCTTACA GGAAGCTGTC TGCTCAAGAC TACCGGTCTG GCATCCACAG AAATTCCCAT
    GGCTCACTAG TCACAGCTGC ACAACGGGAC CAGGCTTCTG TGGGACAGAG CAGTGGTCTC
    AGTGGAGAGC AAAACATAAG GACACACAGG ACCGACTCAG CATCCGACCC CAAGTACAAG
    TCTGACGGCC TGAGTCCCTC ACGCCTTCAC TCAGGAGATG ATCAGACTTC AGGCAGCACC
    CTGGTGAATG GCTCCCGGTG GGTCCCCATC CACAGCGAGC CTCCTTGCTC TCCAAGAGTG
    TCAGATACAA ATGGCCGGGC TGGACAGGAG CCCCCACAAC GGGAGGAAAA GCCCAGTTTG
    TTAAGCACAG GTGCACTTGA AGCTGATGGT ACACTGCCAG CCAGCATCTT CGGGCCAAAG
    AAGAAATTCA GGCCTGTGGT CCAGAGGCCG GCTCCGAAAG ACACATCCCT GCACAGCGCC
    TTGATGGAGG CCATCCACTC AGCAGGAGGC AAGGACAGGC TCCGCAAGAT TCCAGAACCC
    GGCTCAAAGG GAGGGCCAAA GAAACCATCC TACACTGAGG CAGACGGCGA GCGCTCAGCC
    CTCCTGGCGG CCATACGTGG CCACAGCGGG ACCTGCAGCC TGAGGAAGGT CACGTCCTCT
    GCCTCTGAGG AGCTCCAGAG CCTCCGCGAT GCCGCGCTGT CTGCACATGG GCCAGACCCC
    TCGCGGGTAG AAGACCTTGG GGTCCAGTCC CAGCCGCCCC CGCCCCCACC CACTCCGGTT
    ACCCAGGCGC CCACCTCATC CTGGTCAGCG TCGAGGTCCA GCGAGGGCCC CCTCGGCAAC
    CCGCTGGACG CCAGACAGGC CTTGATGGAC GCCATCCGCT CCGGCACCGG AGCCGCAAGG
    CTGAGAAAGC ATTCCTGTCC GATCCTCGCC AAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802268.1
    CDS168..4106
    Translation

    Target ORF information:

    RefSeq Version XM_010803966.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010803966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGAAACC TTTAGAAAAT CTTCACTCAG CAACGATGAG
    ACAGACAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAAT
    AAGGGCTGCT TGACGACCCC GAACTCCCCG TGTGTGAATC CACGTTCTGT TATCCTGGGT
    CCATCCCTCT CTCTTGGCAA CATCTCAGGG GTGTCGGTCA AGTCGGACCT GAAGAAGCGC
    CGGGCACCCC CTCCTCCAAC CCTGCCCCGC GCAGGGCCAC CTGTGCAAGA CAAGACATCA
    GAAAAGATGT CTCTGGGGTC GCAGATTGAC TTACAGAAGA AGAAGAGGCG GGCACCAGCT
    CCCCCTCCAC CACAGCCCCC ACCACCCAGC CCGCTGGTGC CCCCCCGCAC GGAGGACAGG
    GAGGAGGGCA GGAAGGGCAG GACGGGTGTC GGACGGCAGG TGCCACAGAA GCCCCCCCGA
    GGCGCCGCTC GGGCCCCCCC CCAGCTAGTG CTGCCCCCGC CCCCACCCTA CCCGCCTCCT
    GCCGCGGACG CGACCGAGCC TCCGCTTGGC GCTCCCGGGG AAGGCGCTGC TCCCGAGGCC
    TCCAGCCCAA GGCCAGCCCC CGAACTGAGC CTGTCCATGG GGCCCGGCGG TCCCTGCAGT
    GTGGACGGGG CCCCCCCAGT GCCATCGGAG GCTGAGGAGA CCGTGTCTGT CAGCAGCTGC
    TTTGCCTCAG AGGACACCAC AGAGGACTCA GGTGTGATGA GCTCCCCCTC GGACATCGTG
    TCCCTGGATT CCCAGCACGA CAGCATGAAA TCCAAAGACA GGTGGAGCAC AGACCGGGAG
    GACTGCAGCG ACCAGGACCT GGCTGGGACG CCAGAGCCGG GCCCCCCGAA GAGCCCCTTG
    TGGGAGAGGA GCAGCTTTGA GAACCGGCAT CTGAGAAACA GAAAAGCTGC CCCAGCCTCA
    CACGAGGATG AGGAAATATT CATGACTGGA CAGTTCCAGA AAACCCTTGC AGAACTGGAT
    GAAGACCTGG AAGAAATGGA AGAAAGCTAC GAAGCCGACA CAGGCCCCCT CGCCAACTCC
    ATACATGGCA TGTCCACCCC CCATGGTGAC CCTGAGGCAA TCCCGGTGAC GTTCATAGGG
    GAGGTTCTGG ACGATCCCGT GGACTCAGGC GTGTTTTCCA ATAGAAACAA CAACGCCGGC
    TCCTTCGACT TGGGGAGCAC AGCCGACAGT AAGGCTCAGC CACCACCTCA CCAGGCCGAG
    TGCCCCCAGC AGCACAGCCA GAGGCGGGCA GCAGGGCCCA GCTCACCAGC TCCTTCCCGG
    GAGCCTGAGA GAGAGGTCAG GTCAGCCTTG ACCAACACCT GGAAGGAGGT GGATCCCAGG
    AAGATGGAGC CCACGGCGAG TTCTGCTTCA GCGCTTCACA CTCACCACTG GAATGGGAAG
    GAGGAAGGCA GGGCACCTGT TTCTGCTCAC AGCGGGGGGA CCCCAGCCCC TGGAAGGGTG
    ATCCCACAGC TGGGGAAGGA AAAGGCAAGC GATGGTAAAG GTGATATCTC AACACTACCG
    CCATGGCATC AACGAGGCCA ACACCCAGCG GGGAGTTATG GCCTTAAATA TGGCCTCACA
    ACTTATAAAA TTGTCCCTCC GAAGTCAGAG ATGAAATGCT ATGACCGGGG GGCATCTCTC
    TCCGTGGGTG CCATTAAGAT CGATGAGCTG GGGAACCTGG TGAGCCCCCA CGTGCATACC
    GGCAGGACTG TAGTCCCCTC ATCGCCCACT CTGGAGGCAG AAACCCCACC CATTGGAAAA
    GTCAAAGAAT TCTGGAGATC CAATTCTATA GAGAAGCACT CTGGCCGGCC CATGGAGAGG
    CCCAAGAGGA CCCCCACCCC CACCACCCCC ACGAATCTGC AACCTCAGGA AAGCAGACTG
    AGAGGCGAGC CCACCTCCCC AGACCCCCAG GGAACACTGC CCGGGCCCCA GTCCCCACAC
    TCGGAAGATG GGAGAGCTGT GGGGGAGGGG AGAAGCTGGC CACCCCCAGC AGCCACTCGC
    CCCCTCCAAG TGCCAGCAGC TAACCCCGCG GAAGTCCCGT TTCTCAAACC TCAGAGGAGA
    ACATCCAGTC AGTACATGGC CTCTGCCATT GCCAAGCGGA TAGGGACCCC AAAAGTCCAC
    AGTGATGCGG GGAGGAGACA GGATAACACC CAGAGGACGT ATGAAGGCAC AGCACCAGAG
    CCAGTGGTAG CACCTCCTGT GGTGAAGGAC AGTACCACCC CCAGCCCAAA CCCAGGGACG
    GGCATTCGTC GTGAGGGGGA GGAATCGACG GCAGGACCCC ATCCTGGATG GCAAGTCAGC
    AGCCCTTACA GGAAGCTGTC TGCTCAAGAC TACCGGTCTG GCATCCACAG AAATTCCCAT
    GGCTCACTAG TCACAGCTGC ACAACGGGAC CAGGCTTCTG TGGGACAGAG CAGTGGTCTC
    AGTGGAGAGC AAAACATAAG GACACACAGG ACCGACTCAG CATCCGACCC CAAGTACAAG
    TCTGACGGCC TGAGTCCCTC ACGCCTTCAC TCAGGAGATG ATCAGACTTC AGGCAGCACC
    CTGGTGAATG GCTCCCGGTG GGTCCCCATC CACAGCGAGC CTCCTTGCTC TCCAAGAGTG
    TCAGATACAA ATGGCCGGGC TGGACAGGAG CCCCCACAAC GGGAGGAAAA GCCCAGTTTG
    TTAAGCACAG GTGCACTTGA AGCTGATGGT ACACTGCCAG CCAGCATCTT CGGGCCAAAG
    AAGAAATTCA GGCCTGTGGT CCAGAGGCCG GCTCCGAAAG ACACATCCCT GCACAGCGCC
    TTGATGGAGG CCATCCACTC AGCAGGAGGC AAGGACAGGC TCCGCAAGAT TCCAGAACCC
    GGCTCAAAGG GAGGGCCAAA GAAACCATCC TACACTGAGG CAGACGGCGA GCGCTCAGCC
    CTCCTGGCGG CCATACGTGG CCACAGCGGG ACCTGCAGCC TGAGGAAGGT CACGTCCTCT
    GCCTCTGAGG AGCTCCAGAG CCTCCGCGAT GCCGCGCTGT CTGCACATGG GCCAGACCCC
    TCGCGGGTAG AAGACCTTGG GGTCCAGTCC CAGCCGCCCC CGCCCCCACC CACTCCGGTT
    ACCCAGGCGC CCACCTCATC CTGGTCAGCG TCGAGGTCCA GCGAGGGCCC CCTCGGCAAC
    CCGCTGGACG CCAGACAGGC CTTGATGGAC GCCATCCGCT CCGGCACCGG AGCCGCAAGG
    CTGAGAAAGC ATTCCTGTCC GATCCTCGCC AAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55150
    Clone ID Related Accession (Same CDS sequence) XM_010803966.2 , XM_010827200.2
    Accession Version XM_010827200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein cordon-bleu isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644689.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGAAACC TTTAGAAAAT CTTCACTCAG CAACGATGAG
    ACAGACAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAAT
    AAGGGCTGCT TGACGACCCC GAACTCCCCG TGTGTGAATC CACGTTCTGT TATCCTGGGT
    CCATCCCTCT CTCTTGGCAA CATCTCAGGG GTGTCGGTCA AGTCGGACCT GAAGAAGCGC
    CGGGCACCCC CTCCTCCAAC CCTGCCCCGC GCAGGGCCAC CTGTGCAAGA CAAGACATCA
    GAAAAGATGT CTCTGGGGTC GCAGATTGAC TTACAGAAGA AGAAGAGGCG GGCACCAGCT
    CCCCCTCCAC CACAGCCCCC ACCACCCAGC CCGCTGGTGC CCCCCCGCAC GGAGGACAGG
    GAGGAGGGCA GGAAGGGCAG GACGGGTGTC GGACGGCAGG TGCCACAGAA GCCCCCCCGA
    GGCGCCGCTC GGGCCCCCCC CCAGCTAGTG CTGCCCCCGC CCCCACCCTA CCCGCCTCCT
    GCCGCGGACG CGACCGAGCC TCCGCTTGGC GCTCCCGGGG AAGGCGCTGC TCCCGAGGCC
    TCCAGCCCAA GGCCAGCCCC CGAACTGAGC CTGTCCATGG GGCCCGGCGG TCCCTGCAGT
    GTGGACGGGG CCCCCCCAGT GCCATCGGAG GCTGAGGAGA CCGTGTCTGT CAGCAGCTGC
    TTTGCCTCAG AGGACACCAC AGAGGACTCA GGTGTGATGA GCTCCCCCTC GGACATCGTG
    TCCCTGGATT CCCAGCACGA CAGCATGAAA TCCAAAGACA GGTGGAGCAC AGACCGGGAG
    GACTGCAGCG ACCAGGACCT GGCTGGGACG CCAGAGCCGG GCCCCCCGAA GAGCCCCTTG
    TGGGAGAGGA GCAGCTTTGA GAACCGGCAT CTGAGAAACA GAAAAGCTGC CCCAGCCTCA
    CACGAGGATG AGGAAATATT CATGACTGGA CAGTTCCAGA AAACCCTTGC AGAACTGGAT
    GAAGACCTGG AAGAAATGGA AGAAAGCTAC GAAGCCGACA CAGGCCCCCT CGCCAACTCC
    ATACATGGCA TGTCCACCCC CCATGGTGAC CCTGAGGCAA TCCCGGTGAC GTTCATAGGG
    GAGGTTCTGG ACGATCCCGT GGACTCAGGC GTGTTTTCCA ATAGAAACAA CAACGCCGGC
    TCCTTCGACT TGGGGAGCAC AGCCGACAGT AAGGCTCAGC CACCACCTCA CCAGGCCGAG
    TGCCCCCAGC AGCACAGCCA GAGGCGGGCA GCAGGGCCCA GCTCACCAGC TCCTTCCCGG
    GAGCCTGAGA GAGAGGTCAG GTCAGCCTTG ACCAACACCT GGAAGGAGGT GGATCCCAGG
    AAGATGGAGC CCACGGCGAG TTCTGCTTCA GCGCTTCACA CTCACCACTG GAATGGGAAG
    GAGGAAGGCA GGGCACCTGT TTCTGCTCAC AGCGGGGGGA CCCCAGCCCC TGGAAGGGTG
    ATCCCACAGC TGGGGAAGGA AAAGGCAAGC GATGGTAAAG GTGATATCTC AACACTACCG
    CCATGGCATC AACGAGGCCA ACACCCAGCG GGGAGTTATG GCCTTAAATA TGGCCTCACA
    ACTTATAAAA TTGTCCCTCC GAAGTCAGAG ATGAAATGCT ATGACCGGGG GGCATCTCTC
    TCCGTGGGTG CCATTAAGAT CGATGAGCTG GGGAACCTGG TGAGCCCCCA CGTGCATACC
    GGCAGGACTG TAGTCCCCTC ATCGCCCACT CTGGAGGCAG AAACCCCACC CATTGGAAAA
    GTCAAAGAAT TCTGGAGATC CAATTCTATA GAGAAGCACT CTGGCCGGCC CATGGAGAGG
    CCCAAGAGGA CCCCCACCCC CACCACCCCC ACGAATCTGC AACCTCAGGA AAGCAGACTG
    AGAGGCGAGC CCACCTCCCC AGACCCCCAG GGAACACTGC CCGGGCCCCA GTCCCCACAC
    TCGGAAGATG GGAGAGCTGT GGGGGAGGGG AGAAGCTGGC CACCCCCAGC AGCCACTCGC
    CCCCTCCAAG TGCCAGCAGC TAACCCCGCG GAAGTCCCGT TTCTCAAACC TCAGAGGAGA
    ACATCCAGTC AGTACATGGC CTCTGCCATT GCCAAGCGGA TAGGGACCCC AAAAGTCCAC
    AGTGATGCGG GGAGGAGACA GGATAACACC CAGAGGACGT ATGAAGGCAC AGCACCAGAG
    CCAGTGGTAG CACCTCCTGT GGTGAAGGAC AGTACCACCC CCAGCCCAAA CCCAGGGACG
    GGCATTCGTC GTGAGGGGGA GGAATCGACG GCAGGACCCC ATCCTGGATG GCAAGTCAGC
    AGCCCTTACA GGAAGCTGTC TGCTCAAGAC TACCGGTCTG GCATCCACAG AAATTCCCAT
    GGCTCACTAG TCACAGCTGC ACAACGGGAC CAGGCTTCTG TGGGACAGAG CAGTGGTCTC
    AGTGGAGAGC AAAACATAAG GACACACAGG ACCGACTCAG CATCCGACCC CAAGTACAAG
    TCTGACGGCC TGAGTCCCTC ACGCCTTCAC TCAGGAGATG ATCAGACTTC AGGCAGCACC
    CTGGTGAATG GCTCCCGGTG GGTCCCCATC CACAGCGAGC CTCCTTGCTC TCCAAGAGTG
    TCAGATACAA ATGGCCGGGC TGGACAGGAG CCCCCACAAC GGGAGGAAAA GCCCAGTTTG
    TTAAGCACAG GTGCACTTGA AGCTGATGGT ACACTGCCAG CCAGCATCTT CGGGCCAAAG
    AAGAAATTCA GGCCTGTGGT CCAGAGGCCG GCTCCGAAAG ACACATCCCT GCACAGCGCC
    TTGATGGAGG CCATCCACTC AGCAGGAGGC AAGGACAGGC TCCGCAAGAT TCCAGAACCC
    GGCTCAAAGG GAGGGCCAAA GAAACCATCC TACACTGAGG CAGACGGCGA GCGCTCAGCC
    CTCCTGGCGG CCATACGTGG CCACAGCGGG ACCTGCAGCC TGAGGAAGGT CACGTCCTCT
    GCCTCTGAGG AGCTCCAGAG CCTCCGCGAT GCCGCGCTGT CTGCACATGG GCCAGACCCC
    TCGCGGGTAG AAGACCTTGG GGTCCAGTCC CAGCCGCCCC CGCCCCCACC CACTCCGGTT
    ACCCAGGCGC CCACCTCATC CTGGTCAGCG TCGAGGTCCA GCGAGGGCCC CCTCGGCAAC
    CCGCTGGACG CCAGACAGGC CTTGATGGAC GCCATCCGCT CCGGCACCGG AGCCGCAAGG
    CTGAGAAAGC ATTCCTGTCC GATCCTCGCC AAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825502.1
    CDS168..4106
    Translation

    Target ORF information:

    RefSeq Version XM_010827200.2
    Organism Bos taurus(cattle)
    Definition Bos taurus cordon-bleu WH2 repeat protein (COBL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010827200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACGCGT TGCGAGCCTC GGCGGCCAAG CCCCCGACCG GGAGGAAGAT GAAGGCTCGC 
    GCTCCCCCGC CTCCTGGGAA GCCTGCCACC CCCAACTTGC ACAGTGGACA GAGATCTCCT
    CGCAGAGCAT CCCCCGGGCC CCCACAGAAC CAGCTCAGCA GAAAGCATTC GCTGGACGAC
    GGGGTGGTGG CCATGACCGT CGTGCTGCCC AGCGGGCTGG AGAAGAGAAG TGTGGTCAAC
    GGGAGCCATG CGATGATGGA CCTACTGGTT GAACTTTGCC TTCAGAACCA CCTGAATCCC
    TCCAACCATG CCCTGGAGAT CCGGTCTTCA GAAACCCAAC AGCCTTTGAA TTTTAAGCCA
    AATACTTTAG TTGGGACCTT GAATGTGCAC ACCGTATTTC TGAAAGAAAA AGTTCCTGAA
    GCGAAAGTTA AGCCTGTTCC CCCTAAGGTG CCTGAGAAAA CGGTGCGCCT AGTGGTGAAT
    TACCTGCGCA CGCAGAAGGC GGTGGTGCGT GTGAGCCCCG AGGTGCCCCT GCAGAGCATC
    CTCCCGGTCA TCTGCGCCAA GTGTGAGGTC AGCCCAGAGC ACGTGGTGCT CCTCAGGGAC
    AATGTGGCTG GTGAGGAGCT GGAGCTTTCC AAGTCCCTGA ACGAGCTGGG GATAAAGGAG
    TTGTATGCCT GGGACAACAA GAGAGAAACC TTTAGAAAAT CTTCACTCAG CAACGATGAG
    ACAGACAAAG AGAAGAAAAA ATTTCTGGGA TTTTTCAAAG TTAATAAAAG AAGCAATAAT
    AAGGGCTGCT TGACGACCCC GAACTCCCCG TGTGTGAATC CACGTTCTGT TATCCTGGGT
    CCATCCCTCT CTCTTGGCAA CATCTCAGGG GTGTCGGTCA AGTCGGACCT GAAGAAGCGC
    CGGGCACCCC CTCCTCCAAC CCTGCCCCGC GCAGGGCCAC CTGTGCAAGA CAAGACATCA
    GAAAAGATGT CTCTGGGGTC GCAGATTGAC TTACAGAAGA AGAAGAGGCG GGCACCAGCT
    CCCCCTCCAC CACAGCCCCC ACCACCCAGC CCGCTGGTGC CCCCCCGCAC GGAGGACAGG
    GAGGAGGGCA GGAAGGGCAG GACGGGTGTC GGACGGCAGG TGCCACAGAA GCCCCCCCGA
    GGCGCCGCTC GGGCCCCCCC CCAGCTAGTG CTGCCCCCGC CCCCACCCTA CCCGCCTCCT
    GCCGCGGACG CGACCGAGCC TCCGCTTGGC GCTCCCGGGG AAGGCGCTGC TCCCGAGGCC
    TCCAGCCCAA GGCCAGCCCC CGAACTGAGC CTGTCCATGG GGCCCGGCGG TCCCTGCAGT
    GTGGACGGGG CCCCCCCAGT GCCATCGGAG GCTGAGGAGA CCGTGTCTGT CAGCAGCTGC
    TTTGCCTCAG AGGACACCAC AGAGGACTCA GGTGTGATGA GCTCCCCCTC GGACATCGTG
    TCCCTGGATT CCCAGCACGA CAGCATGAAA TCCAAAGACA GGTGGAGCAC AGACCGGGAG
    GACTGCAGCG ACCAGGACCT GGCTGGGACG CCAGAGCCGG GCCCCCCGAA GAGCCCCTTG
    TGGGAGAGGA GCAGCTTTGA GAACCGGCAT CTGAGAAACA GAAAAGCTGC CCCAGCCTCA
    CACGAGGATG AGGAAATATT CATGACTGGA CAGTTCCAGA AAACCCTTGC AGAACTGGAT
    GAAGACCTGG AAGAAATGGA AGAAAGCTAC GAAGCCGACA CAGGCCCCCT CGCCAACTCC
    ATACATGGCA TGTCCACCCC CCATGGTGAC CCTGAGGCAA TCCCGGTGAC GTTCATAGGG
    GAGGTTCTGG ACGATCCCGT GGACTCAGGC GTGTTTTCCA ATAGAAACAA CAACGCCGGC
    TCCTTCGACT TGGGGAGCAC AGCCGACAGT AAGGCTCAGC CACCACCTCA CCAGGCCGAG
    TGCCCCCAGC AGCACAGCCA GAGGCGGGCA GCAGGGCCCA GCTCACCAGC TCCTTCCCGG
    GAGCCTGAGA GAGAGGTCAG GTCAGCCTTG ACCAACACCT GGAAGGAGGT GGATCCCAGG
    AAGATGGAGC CCACGGCGAG TTCTGCTTCA GCGCTTCACA CTCACCACTG GAATGGGAAG
    GAGGAAGGCA GGGCACCTGT TTCTGCTCAC AGCGGGGGGA CCCCAGCCCC TGGAAGGGTG
    ATCCCACAGC TGGGGAAGGA AAAGGCAAGC GATGGTAAAG GTGATATCTC AACACTACCG
    CCATGGCATC AACGAGGCCA ACACCCAGCG GGGAGTTATG GCCTTAAATA TGGCCTCACA
    ACTTATAAAA TTGTCCCTCC GAAGTCAGAG ATGAAATGCT ATGACCGGGG GGCATCTCTC
    TCCGTGGGTG CCATTAAGAT CGATGAGCTG GGGAACCTGG TGAGCCCCCA CGTGCATACC
    GGCAGGACTG TAGTCCCCTC ATCGCCCACT CTGGAGGCAG AAACCCCACC CATTGGAAAA
    GTCAAAGAAT TCTGGAGATC CAATTCTATA GAGAAGCACT CTGGCCGGCC CATGGAGAGG
    CCCAAGAGGA CCCCCACCCC CACCACCCCC ACGAATCTGC AACCTCAGGA AAGCAGACTG
    AGAGGCGAGC CCACCTCCCC AGACCCCCAG GGAACACTGC CCGGGCCCCA GTCCCCACAC
    TCGGAAGATG GGAGAGCTGT GGGGGAGGGG AGAAGCTGGC CACCCCCAGC AGCCACTCGC
    CCCCTCCAAG TGCCAGCAGC TAACCCCGCG GAAGTCCCGT TTCTCAAACC TCAGAGGAGA
    ACATCCAGTC AGTACATGGC CTCTGCCATT GCCAAGCGGA TAGGGACCCC AAAAGTCCAC
    AGTGATGCGG GGAGGAGACA GGATAACACC CAGAGGACGT ATGAAGGCAC AGCACCAGAG
    CCAGTGGTAG CACCTCCTGT GGTGAAGGAC AGTACCACCC CCAGCCCAAA CCCAGGGACG
    GGCATTCGTC GTGAGGGGGA GGAATCGACG GCAGGACCCC ATCCTGGATG GCAAGTCAGC
    AGCCCTTACA GGAAGCTGTC TGCTCAAGAC TACCGGTCTG GCATCCACAG AAATTCCCAT
    GGCTCACTAG TCACAGCTGC ACAACGGGAC CAGGCTTCTG TGGGACAGAG CAGTGGTCTC
    AGTGGAGAGC AAAACATAAG GACACACAGG ACCGACTCAG CATCCGACCC CAAGTACAAG
    TCTGACGGCC TGAGTCCCTC ACGCCTTCAC TCAGGAGATG ATCAGACTTC AGGCAGCACC
    CTGGTGAATG GCTCCCGGTG GGTCCCCATC CACAGCGAGC CTCCTTGCTC TCCAAGAGTG
    TCAGATACAA ATGGCCGGGC TGGACAGGAG CCCCCACAAC GGGAGGAAAA GCCCAGTTTG
    TTAAGCACAG GTGCACTTGA AGCTGATGGT ACACTGCCAG CCAGCATCTT CGGGCCAAAG
    AAGAAATTCA GGCCTGTGGT CCAGAGGCCG GCTCCGAAAG ACACATCCCT GCACAGCGCC
    TTGATGGAGG CCATCCACTC AGCAGGAGGC AAGGACAGGC TCCGCAAGAT TCCAGAACCC
    GGCTCAAAGG GAGGGCCAAA GAAACCATCC TACACTGAGG CAGACGGCGA GCGCTCAGCC
    CTCCTGGCGG CCATACGTGG CCACAGCGGG ACCTGCAGCC TGAGGAAGGT CACGTCCTCT
    GCCTCTGAGG AGCTCCAGAG CCTCCGCGAT GCCGCGCTGT CTGCACATGG GCCAGACCCC
    TCGCGGGTAG AAGACCTTGG GGTCCAGTCC CAGCCGCCCC CGCCCCCACC CACTCCGGTT
    ACCCAGGCGC CCACCTCATC CTGGTCAGCG TCGAGGTCCA GCGAGGGCCC CCTCGGCAAC
    CCGCTGGACG CCAGACAGGC CTTGATGGAC GCCATCCGCT CCGGCACCGG AGCCGCAAGG
    CTGAGAAAGC ATTCCTGTCC GATCCTCGCC AAGGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.