MARCKS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MARCKS
Entrez Gene ID	613548
Full Name	myristoylated alanine rich protein kinase C substrate
General protein information	Preferred Names myristoylated alanine rich protein kinase C substrate Names myristoylated alanine-rich C-kinase substrate ACAMP-81
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001076276.1	NP_001069744.1	myristoylated alanine-rich C-kinase substrate

Pathways from BioSystems

Homologies

Homo sapiens (human)	MARCKS	NP_002347.5
Pan troglodytes (chimpanzee)	MARCKS	XP_001159872.1
	MARCKS	XP_001082393.1
Bos taurus (cattle)	MARCKS	NP_001069744.1

Gene Ontology
Bibliography

Related articles in PubMed

MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.
Jin BY, Lin AJ, Golan DE, Michel T
Proceedings of the National Academy of Sciences of the United States of America109(37)14864-9(2012 Sep)

Angiotensin-II and MARCKS: a hydrogen peroxide- and RAC1-dependent signaling pathway in vascular endothelium.
Kalwa H, Sartoretto JL, Sartoretto SM, Michel T
The Journal of biological chemistry287(34)29147-58(2012 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Protein kinase C mediated inhibition of endothelial L-arginine transport is mediated by MARCKS protein.
Venardos K, Enriquez C, Marshall T, Chin-Dusting JP, Ahlers B, Kaye DM
Journal of molecular and cellular cardiology46(1)86-92(2009 Jan)

Actin filament assembly by myristoylated alanine-rich C kinase substrate-phosphatidylinositol-4,5-diphosphate signaling is critical for dendrite branching.
Li H, Chen G, Zhou B, Duan S
Molecular biology of the cell19(11)4804-13(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.
Title: MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.

a critical role for H(2)O(2) in angiotensin-II signaling to the endothelial cytoskeleton in a novel pathway that is critically dependent on MARCKS, Rac1, and c-Abl.
Title: Angiotensin-II and MARCKS: a hydrogen peroxide- and RAC1-dependent signaling pathway in vascular endothelium.

Protein kinase C mediated inhibition of endothelial L-arginine transport is mediated by MARCKS protein
Title: Protein kinase C mediated inhibition of endothelial L-arginine transport is mediated by MARCKS protein.

These findings demonstrate a critical role for MARCKS-phosphatidylinositol-4,5-diphosphate signaling in regulating dendrite development.
Title: Actin filament assembly by myristoylated alanine-rich C kinase substrate-phosphatidylinositol-4,5-diphosphate signaling is critical for dendrite branching.

The following MARCKS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MARCKS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa42749	NM_001076276.1 Latest version!	Bos taurus myristoylated alanine rich protein kinase C substrate (MARCKS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MARCKS ( NM_001076276.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001069744.1 Bos taurus myristoylated alanine rich protein kinase C substrate (MARCKS), mRNA.

Price & Availability↑

CloneID	OBa42749
Clone ID Related Accession (Same CDS sequence)	NM_001076276.1
Accession Version	NM_001076276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	myristoylated alanine-rich C-kinase substrate
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M24638.1. On or before Oct 4, 2006 this sequence version replaced XM_864035.1, XM_872124.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC115989.1, EH131232.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGCCC AGTTCTCCAA GACCGCCGCG AAGGGAGAAG CCACCGCGGA GAGGCCCGGG 
GAGGCGGCTG TGGCCTCGTC GCCTTCTAAA GCGAATGGGC AGGAAAATGG CCACGTGAAG 
GTAAACGGCG ATGCTTCTCC CGCGGCCGCC GAGCCCGGCG CCAAGGAGGA GCTGCAGGCC 
AACGGCAGCG CCCCGGCGGC CGACAAGGAG GAGCCCGCGG CCGCCGGGAG TGGGGCAGCG 
TCGCCTGCCG CGGCCGAGAA AGATGAGCCG GCCGCCGCTG CCCCCGACGC CGGCCACCCC 
GTGGAGAAGG AGGCCCCCGT GGAGGGCGGA GCCGCGGAGC CCGGCTCACC CACGGCCGCC 
GAGGGGGAGG CCGCCTCTGC CGCCTCCTCG AGCTCTTCGC CGAAGGCTGA GGACGGGGGC 
CACGCCCTCG CCCAGCAACG AGACGCCGAA AAAAAAAAAA AGAAGCGCTT TTCCTTCAAG 
AAGTCTTTCA AGCTGAGCGG CTTCTCCTTC AAGAAGAACA AGAAGGAGGC AGGAGAGGGC 
GGTGAGGCCG AAGGGGCCGC CGGCGCCTCC GCCGAAGGCG GCAAGGACGA GGCCTCTGGG 
GGCGCCGCTG CGGCCGCCGG CGAGGCGGGT GCGGCCCCCG GGGAGCCGAC AGCGGCGCCA 
GGCGAGGAGG CGGCCGCGGG CGAGGAGGGG GCGGCGGGGG GCGACCCGCA GGAGGCCAAG 
CCCGAGGAGG CTGCCGTCGC GCCCGAGAAG CCGCCCGCCA GGCGAGGAGC TAAGGCCGTC 
GAGGAGCCCA GCAAGGCTGA AGAGAAGGCA GAAGAGGCCG GGGTCAGCGC GGCCGGCGCG 
GCCGGCTGCG AGGCGCCCTC GGCCGCCGGG CCGGGCTGCC CCAGAGCAGG AGGCGCCCCC 
CGTGAGGAGG CAGCGCCGCC CCGGGCGTCG TCAGCCTGCT CAGCCCCCTC ACAGGAGGCC 
CAGCCCGAGT GCAGTCCAGA AGCGCCCCCA GCGGAGGCAG CCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001069744.1
CDS	1..1008
Translation	MGAQFSKTAAKGEATAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAEPGAKEELQANGSAPAADKEEPAAAGSGAASPAAAEKDEPAAAAPDAGHPVEKEAPVEGGAAEPGSPTAAEGEAASAASSSSSPKAEDGGHALAQQRDAEKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGGEAEGAAGASAEGGKDEASGGAAAAAGEAGAAPGEPTAAPGEEAAAGEEGAAGGDPQEAKPEEAAVAPEKPPARRGAKAVEEPSKAEEKAEEAGVSAAGAAGCEAPSAAGPGCPRAGGAPREEAAPPRASSACSAPSQEAQPECSPEAPPAEAAE

Target ORF information:

RefSeq Version	NM_001076276.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myristoylated alanine rich protein kinase C substrate (MARCKS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001076276.1

ORF Insert Sequence:

ATGGGTGCCC AGTTCTCCAA GACCGCCGCG AAGGGAGAAG CCACCGCGGA GAGGCCCGGG 
GAGGCGGCTG TGGCCTCGTC GCCTTCTAAA GCGAATGGGC AGGAAAATGG CCACGTGAAG 
GTAAACGGCG ATGCTTCTCC CGCGGCCGCC GAGCCCGGCG CCAAGGAGGA GCTGCAGGCC 
AACGGCAGCG CCCCGGCGGC CGACAAGGAG GAGCCCGCGG CCGCCGGGAG TGGGGCAGCG 
TCGCCTGCCG CGGCCGAGAA AGATGAGCCG GCCGCCGCTG CCCCCGACGC CGGCCACCCC 
GTGGAGAAGG AGGCCCCCGT GGAGGGCGGA GCCGCGGAGC CCGGCTCACC CACGGCCGCC 
GAGGGGGAGG CCGCCTCTGC CGCCTCCTCG AGCTCTTCGC CGAAGGCTGA GGACGGGGGC 
CACGCCCTCG CCCAGCAACG AGACGCCGAA AAAAAAAAAA AGAAGCGCTT TTCCTTCAAG 
AAGTCTTTCA AGCTGAGCGG CTTCTCCTTC AAGAAGAACA AGAAGGAGGC AGGAGAGGGC 
GGTGAGGCCG AAGGGGCCGC CGGCGCCTCC GCCGAAGGCG GCAAGGACGA GGCCTCTGGG 
GGCGCCGCTG CGGCCGCCGG CGAGGCGGGT GCGGCCCCCG GGGAGCCGAC AGCGGCGCCA 
GGCGAGGAGG CGGCCGCGGG CGAGGAGGGG GCGGCGGGGG GCGACCCGCA GGAGGCCAAG 
CCCGAGGAGG CTGCCGTCGC GCCCGAGAAG CCGCCCGCCA GGCGAGGAGC TAAGGCCGTC 
GAGGAGCCCA GCAAGGCTGA AGAGAAGGCA GAAGAGGCCG GGGTCAGCGC GGCCGGCGCG 
GCCGGCTGCG AGGCGCCCTC GGCCGCCGGG CCGGGCTGCC CCAGAGCAGG AGGCGCCCCC 
CGTGAGGAGG CAGCGCCGCC CCGGGCGTCG TCAGCCTGCT CAGCCCCCTC ACAGGAGGCC 
CAGCCCGAGT GCAGTCCAGA AGCGCCCCCA GCGGAGGCAG CCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.
Proceedings of the National Academy of Sciences of the United States of America109(37)14864-9(2012 Sep)
Jin BY,Lin AJ,Golan DE,Michel T

Angiotensin-II and MARCKS: a hydrogen peroxide- and RAC1-dependent signaling pathway in vascular endothelium.
The Journal of biological chemistry287(34)29147-58(2012 Aug)
Kalwa H,Sartoretto JL,Sartoretto SM,Michel T

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Protein kinase C mediated inhibition of endothelial L-arginine transport is mediated by MARCKS protein.
Journal of molecular and cellular cardiology46(1)86-92(2009 Jan)
Venardos K,Enriquez C,Marshall T,Chin-Dusting JP,Ahlers B,Kaye DM

Actin filament assembly by myristoylated alanine-rich C kinase substrate-phosphatidylinositol-4,5-diphosphate signaling is critical for dendrite branching.
Molecular biology of the cell19(11)4804-13(2008 Nov)
Li H,Chen G,Zhou B,Duan S