AFF4 cDNA ORF clone, Bos taurus(cattle)

The following AFF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa21434 NM_001191129.1
Latest version!
Bos taurus AF4/FMR2 family member 4 (AFF4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa21434 XM_024994567.1
Latest version!
Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa21434 XM_005209304.4
Latest version!
Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa21435 XM_010807254.3
Latest version!
Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa21434 XM_005209304.3 Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa21435 XM_010807254.2 Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa21434
    Clone ID Related Accession (Same CDS sequence) NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
    Accession Version NM_001191129.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On Jul 17, 2010 this sequence version replaced XM_001249664.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.23887.1, SRR5571260.50474.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178058.1
    CDS170..3664
    Translation

    Target ORF information:

    RefSeq Version NM_001191129.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 4 (AFF4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21434
    Clone ID Related Accession (Same CDS sequence) NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
    Accession Version XM_005209304.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209304.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209361.1
    CDS365..3859
    Translation

    Target ORF information:

    RefSeq Version XM_005209304.4
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209304.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21434
    Clone ID Related Accession (Same CDS sequence) NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
    Accession Version XM_005209304.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209361.1
    CDS367..3861
    Translation

    Target ORF information:

    RefSeq Version XM_005209304.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209304.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21434
    Clone ID Related Accession (Same CDS sequence) NM_001191129.1 , XM_005209304.4 , XM_005209304.3 , XM_024994567.1
    Accession Version XM_024994567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850335.1
    CDS24..3518
    Translation

    Target ORF information:

    RefSeq Version XM_024994567.1
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024994567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGATGA CGCATTCATG GCCTCCCCCT CTAACGGCTA TTCATACACC ATGCAAAACA
    GAACCTTCCA AATTTCCTTT TCCAACTAAG GAGTCTCAGC AGTCCAGTTT TGGCCCTGGA
    GAACAAAAAA GATATAATCC TTCTTCTAAA ACTTCAAATG CACACCAGTC TAAATCTATG
    TTAAAAGATG ACTTAAAGCT AAGCAGCAGT GAAGACAGTG ATGGAGAACA GGATTGTGAT
    AAGACAATGC CAAGGAGTAC ACCAGGAAGT AACTCTGAAC CTTCACACCA TAATAGTGAA
    GGGGCAGATA ACTCCAGGGA TGATTCTAGC AGCCACAGTG GATCTGAAAG CAGCTCTGGA
    TCTGACTCAG AGAGTGAAAG TAGTTCCAGT GACAGTGAGG CAAATGAGCC ATCCCAGAGT
    GCATCTCCTG AGCCTGAACC ACCTCCCACA AATAAATGGC AACTTGATAA CTGGCTGAAT
    AAAGTAAATC CACATAAAGT ATCACCAGCT TCTTCTGTGG ACAGTAATAT CCCATCATCT
    CAAGGCTATA AAAAGGAAGG CCGAGAACAG GGCACAGGGA ATAGCTACAC AGATCCAGGT
    GGTCCTAAAG AAACAAGTTC TGCTACTCCT GGACGAGACT CCAAAACTGT CCAAAAGGGA
    TCAGAAAGTG GGCGTGGGAG GCAAAAGTCT CCTGCCCAGA GTGACAGCAC AGCACAGAGG
    AGAACTGTAG GGAAAAAACA ACCCAAAAAG ACTGAGAAGG CAGCTGCTGA AGAGCCCCGT
    GGAGGCCTGA AGATAGAGAG TGAAACTCCT GTAGACATGG CTACCAGCAT GCCCTCCAGC
    AGACACAAAG CGGCCACCAA AGGCTCAAGA AAACCCAATA TAAAAAAGGA GTCCAAATCT
    TCCCCTCGAC CTTCAGCAGA GAAAAAGAAA TACAAGTCAA CAAGTAAATC TTCCCAGAAA
    TCAAGGGAAA TTATAGAAAC AGATACCTCA TCCTCAGATT CGGATGAAAG CGAGAGCCTT
    CCTCCTTCCT CACAAACTCC TAAGTACCCT GAGAGTAATA GGACTCCCGT TAAACCCTCC
    TCAGTGGAGG AAGAAGATAG CTTTTTTCGG CAACGAATGT TCTCTCCTAT GGAAGAGAAG
    GAACTTCTTT CACCCCTCAG TGAGCCTGAT GACAGGTATC CACTTATTGT GAAGATTGAC
    CTGAATCTCT TGACTAGAAT ACCAGGAAAG CCTTACAAAG AAACAGAGCC ACCCAAGGGG
    GAAAAGAAAA ATGTGCCAGA AAAGCACACA AGAGAGGCTC AGAAACAAGC CTCAGAGAAA
    GTTTCCAACA AAGGCAAGAG GAAACATAAG AATGAAGATG ATAACCGAGC CAATGAAAGC
    AAGAAACCTA AAACAGAGGA CAAGAATATG GCAGGCCACA AGCCATCCAG CAACAAAGAG
    TCATCTAAAC AGAGTGCTTC TAAAGAAAAA GATTTATTGC CTTCTCCTGC TGGGCCTGTT
    CCTTCAAAAG ATCCAAAAAC AGAGCATGGC TCTCGGAAGA GGACTGTTAG TCAGTCTTCT
    TCCTTAAAGT CAAGTAATAA CAGCAACAAG GAAAATAGTG CCAGCAGCAA AAACAGTTCC
    TCCGCATCAA AGCAGAAGAG GACTGAAGGG AAGACTTCCA GTAGCTCTAA GGAGGTCAAG
    GAAAAAGCTC CAAATAGTTC TTCTAACTGT CCTCCATCTG CACCAACTCC TGATGCTTCT
    AAGCCTCGGA GAACGAAGCT TGCCTTTGAT GACAGAAATT ATTCAGCAGA CCATTATTTA
    CAAGAAGCAA AAAAGCTAAA GCACAATGCA GATGCATTGT CTGATAGGTT TGAGAAAGCT
    GTATACTATC TTGATGCTGT GGTATCTTTC ATTGAATGTG GGAATGCATT AGAGAAAAAT
    GCTCAGGAAT CCAAATCCCC ATTCCTTATG TATTCAGAAA CGGTGGAGCT CATCAAATAC
    ACTATGAAGC TAAAGAATTA CTTGGCACCA GATGCTACAG CTGCAGATAA AAGACTTACA
    GTGCTTTGCC TGCGATGCCA GTCTTTGCTG TACCTGAGGC TCTTCAAACT GAAGAAAGAA
    ACTGCTCTGA AGTATTCAAA GACACTGACA GAGCATCTGA AGAATTCTTA CAATAACTCT
    CAAGCACCAT CACCAGGCTT GGGAAGCAAA GCTGTTGGGA TGCCTTCCCC TGTTTCTCCA
    AAGCTGTCAC CAGGCAATTC AGGAAATTAT TCTTCTGGAG CCAGTAGTGC TTCAGCAAGT
    GGTTCTTCAG TGACCATTCC ACAGAGGATT CACCACATGG CAGCCAGCTA TGTTCAGGTT
    ACATCCAACT TCCTCTATGC CACTGAAATT TGGGACCAAG CTGAACAGCT TTCCAAAGAG
    CAAAAAGAAT TCTTTGCTGA ACTGGATAAA GTAATGGGCC CTCTCATCTT TAATGCAAGC
    ATCATGACAG ACCTAGTTCG TTACACCCGG CAGGGACTGC ACTGGCTTCG CCAGGATGCC
    AAGTTGATAT CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21435
    Clone ID Related Accession (Same CDS sequence) XM_010807254.2 , XM_010807254.3
    Accession Version XM_010807254.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807254.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT
    AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC
    AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA
    AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT
    AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC
    AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC
    ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA
    GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA
    CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT
    CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG
    TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA
    AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT
    CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA
    AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG
    AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC
    TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC
    CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT
    CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT
    GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA
    AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC
    ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT
    AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT
    ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA
    AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT
    GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC
    AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA
    GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC
    TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT
    GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT
    GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT
    TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT
    ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA
    CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG
    CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG
    ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC
    AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT
    TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG
    ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT
    AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC
    CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805556.1
    CDS367..3774
    Translation

    Target ORF information:

    RefSeq Version XM_010807254.2
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807254.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT
    AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC
    AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA
    AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT
    AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC
    AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC
    ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA
    GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA
    CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT
    CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG
    TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA
    AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT
    CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA
    AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG
    AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC
    TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC
    CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT
    CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT
    GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA
    AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC
    ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT
    AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT
    ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA
    AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT
    GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC
    AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA
    GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC
    TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT
    GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT
    GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT
    TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT
    ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA
    CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG
    CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG
    ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC
    AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT
    TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG
    ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT
    AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC
    CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21435
    Clone ID Related Accession (Same CDS sequence) XM_010807254.2 , XM_010807254.3
    Accession Version XM_010807254.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product AF4/FMR2 family member 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT
    AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC
    AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA
    AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT
    AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC
    AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC
    ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA
    GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA
    CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT
    CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG
    TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA
    AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT
    CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA
    AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG
    AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC
    TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC
    CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT
    CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT
    GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA
    AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC
    ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT
    AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT
    ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA
    AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT
    GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC
    AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA
    GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC
    TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT
    GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT
    GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT
    TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT
    ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA
    CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG
    CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG
    ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC
    AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT
    TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG
    ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT
    AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC
    CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805556.1
    CDS364..3771
    Translation

    Target ORF information:

    RefSeq Version XM_010807254.3
    Organism Bos taurus(cattle)
    Definition Bos taurus AF4/FMR2 family member 4 (AFF4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807254.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACCGTG AAGACCGGAA TGTGCTGCGT ATGAAAGAAC GGGAAAGGCG GAATCAGGAA 
    ATTCAGCAGG GCGACGACGC CTTCCCACCT AGCTCTCCTC TCTTTGCTGA GCCATACAAA
    GTTACTAGCA AAGAAGATAA GCTGTCAAGT CGTATTCAGA GTATGCTTGG AAACTACGAT
    GAAATGAAGG ATTTCATAGG AGACAGATCT ATACCAAAGC TTGTTGCAAT TCCCAAGCCT
    ACAGTACCGC CGACAGCAGA TGAAAAATCT AACCCAAATT TCTTTGAACA GAGACATGGG
    AGCTCTCATC AGAGTAGCAA ATGGACTCCA GTAGGACCTG CACCCAGCAC TTCTCAGTCT
    CAGAAAAGGT CCTCGGGCTT ACAGAGTGGA CACAGTAGCC AGCGGGCCAG TGCAGGTGCC
    AGTGGTGGCA CTAATAGTAG TGGCCAGAGA CATGACCGTG ACTCATACAG CAGTGGTGGG
    AGCAGTAGCC GAAAAAAAGG CCAGCATGGA TCAGAACACT CCAAATCACG CTCTTCCAGC
    CCTGGAAAAC CCCAGGCTGT TTCTTCATTA AGCTCCAGTC ATTCCAGGTC TCATGGGAAT
    GATCACCATA GCAAAGAACA TCAGCGTTCC AAATCACCTC GGGACCCTGA TGCAAACTGG
    GATTCTCCTT CCCGTGTACC TTTTTCAAGT GGGCAGCACT CAAATCAGTC TTTCCCACCT
    TCATTGATGT CAAAGTCCAG CTCAATGCTA CAGAAACCCA CTGCCTATGT GCGGCCCATG
    GATGGACAGG AGTCCATGGA ACCAAAGCTG TCCTCTGAAC ACTACAGCAG CCAGTCTCAT
    GGCAACAGCA TGACTGAACT GAAGCCCAGC AGTAAAGCAC ATCTCACCAA GCTGAAAATA
    CCTTCCCAAC CATTGGATGC ATCTGCTTCT GGGGATGTGA GTTGTGTGGA TGAAATTCTT
    AAAGAGTCTC AGCAGTCCAG TTTTGGCCCT GGAGAACAAA AAAGATATAA TCCTTCTTCT
    AAAACTTCAA ATGCACACCA GTCTAAATCT ATGTTAAAAG ATGACTTAAA GCTAAGCAGC
    AGTGAAGACA GTGATGGAGA ACAGGATTGT GATAAGACAA TGCCAAGGAG TACACCAGGA
    AGTAACTCTG AACCTTCACA CCATAATAGT GAAGGGGCAG ATAACTCCAG GGATGATTCT
    AGCAGCCACA GTGGATCTGA AAGCAGCTCT GGATCTGACT CAGAGAGTGA AAGTAGTTCC
    AGTGACAGTG AGGCAAATGA GCCATCCCAG AGTGCATCTC CTGAGCCTGA ACCACCTCCC
    ACAAATAAAT GGCAACTTGA TAACTGGCTG AATAAAGTAA ATCCACATAA AGTATCACCA
    GCTTCTTCTG TGGACAGTAA TATCCCATCA TCTCAAGGCT ATAAAAAGGA AGGCCGAGAA
    CAGGGCACAG GGAATAGCTA CACAGATCCA GGTGGTCCTA AAGAAACAAG TTCTGCTACT
    CCTGGACGAG ACTCCAAAAC TGTCCAAAAG GGATCAGAAA GTGGGCGTGG GAGGCAAAAG
    TCTCCTGCCC AGAGTGACAG CACAGCACAG AGGAGAACTG TAGGGAAAAA ACAACCCAAA
    AAGACTGAGA AGGCAGCTGC TGAAGAGCCC CGTGGAGGCC TGAAGATAGA GAGTGAAACT
    CCTGTAGACA TGGCTACCAG CATGCCCTCC AGCAGACACA AAGCGGCCAC CAAAGGCTCA
    AGAAAACCCA ATATAAAAAA GGAGTCCAAA TCTTCCCCTC GACCTTCAGC AGAGAAAAAG
    AAATACAAGT CAACAAGTAA ATCTTCCCAG AAATCAAGGG AAATTATAGA AACAGATACC
    TCATCCTCAG ATTCGGATGA AAGCGAGAGC CTTCCTCCTT CCTCACAAAC TCCTAAGTAC
    CCTGAGAGTA ATAGGACTCC CGTTAAACCC TCCTCAGTGG AGGAAGAAGA TAGCTTTTTT
    CGGCAACGAA TGTTCTCTCC TATGGAAGAG AAGGAACTTC TTTCACCCCT CAGTGAGCCT
    GATGACAGGT ATCCACTTAT TGTGAAGATT GACCTGAATC TCTTGACTAG AATACCAGGA
    AAGCCTTACA AAGAAACAGA GCCACCCAAG GGGGAAAAGA AAAATGTGCC AGAAAAGCAC
    ACAAGAGAGG CTCAGAAACA AGCCTCAGAG AAAGTTTCCA ACAAAGGCAA GAGGAAACAT
    AAGAATGAAG ATGATAACCG AGCCAATGAA AGCAAGAAAC CTAAAACAGA GGACAAGAAT
    ATGGCAGGCC ACAAGCCATC CAGCAACAAA GAGTCATCTA AACAGAGTGC TTCTAAAGAA
    AAAGATTTAT TGCCTTCTCC TGCTGGGCCT GTTCCTTCAA AAGATCCAAA AACAGAGCAT
    GGCTCTCGGA AGAGGACTGT TAGTCAGTCT TCTTCCTTAA AGTCAAGTAA TAACAGCAAC
    AAGGAAAATA GTGCCAGCAG CAAAAACAGT TCCTCCGCAT CAAAGCAGAA GAGGACTGAA
    GGGAAGACTT CCAGTAGCTC TAAGGAGGTC AAGGAAAAAG CTCCAAATAG TTCTTCTAAC
    TGTCCTCCAT CTGCACCAAC TCCTGATGCT TCTAAGCCTC GGAGAACGAA GCTTGCCTTT
    GATGACAGAA ATTATTCAGC AGACCATTAT TTACAAGAAG CAAAAAAGCT AAAGCACAAT
    GCAGATGCAT TGTCTGATAG GTTTGAGAAA GCTGTATACT ATCTTGATGC TGTGGTATCT
    TTCATTGAAT GTGGGAATGC ATTAGAGAAA AATGCTCAGG AATCCAAATC CCCATTCCTT
    ATGTATTCAG AAACGGTGGA GCTCATCAAA TACACTATGA AGCTAAAGAA TTACTTGGCA
    CCAGATGCTA CAGCTGCAGA TAAAAGACTT ACAGTGCTTT GCCTGCGATG CCAGTCTTTG
    CTGTACCTGA GGCTCTTCAA ACTGAAGAAA GAAACTGCTC TGAAGTATTC AAAGACACTG
    ACAGAGCATC TGAAGAATTC TTACAATAAC TCTCAAGCAC CATCACCAGG CTTGGGAAGC
    AAAGCTGTTG GGATGCCTTC CCCTGTTTCT CCAAAGCTGT CACCAGGCAA TTCAGGAAAT
    TATTCTTCTG GAGCCAGTAG TGCTTCAGCA AGTGGTTCTT CAGTGACCAT TCCACAGAGG
    ATTCACCACA TGGCAGCCAG CTATGTTCAG GTTACATCCA ACTTCCTCTA TGCCACTGAA
    ATTTGGGACC AAGCTGAACA GCTTTCCAAA GAGCAAAAAG AATTCTTTGC TGAACTGGAT
    AAAGTAATGG GCCCTCTCAT CTTTAATGCA AGCATCATGA CAGACCTAGT TCGTTACACC
    CGGCAGGGAC TGCACTGGCT TCGCCAGGAT GCCAAGTTGA TATCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.