RSF1 cDNA ORF clone, Bos taurus(cattle)

The following RSF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RSF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa12597 XM_005197281.3
Latest version!
Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa12597 XM_005226727.4
Latest version!
Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa12598 XM_864539.7
Latest version!
Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa12598 XM_002699063.5
Latest version!
Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa55069 XM_010826221.2
Latest version!
Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa55069 XM_010820856.3
Latest version!
Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa12597 XM_005226727.3 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa55069 XM_010820856.2 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa12598 XM_002699063.4 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa12597
    Clone ID Related Accession (Same CDS sequence) XM_005197281.3 , XM_005226727.4 , XM_005226727.3
    Accession Version XM_005197281.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646776.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT
    CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG
    CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA
    GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT
    TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT
    GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT
    AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA
    GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG
    ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA
    CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG
    GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG
    GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG
    ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC
    ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT
    CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG
    ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT
    GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC
    TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC
    CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT
    CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG
    GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA
    GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA
    ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG
    ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA
    TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA
    AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT
    TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG
    AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA
    GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA
    GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG
    GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT
    CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA
    AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA
    GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG
    TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT
    CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA
    GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA
    GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT
    TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT
    GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA
    GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA
    GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT
    GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC
    CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA
    TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG
    AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA
    AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG
    AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA
    AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA
    AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG
    AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT
    AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG
    CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA
    GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT
    TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG
    CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC
    TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC
    ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG
    CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT
    GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC
    CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005197338.1
    CDS284..4639
    Translation

    Target ORF information:

    RefSeq Version XM_005197281.3
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005197281.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT
    CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG
    CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA
    GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT
    TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT
    GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT
    AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA
    GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG
    ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA
    CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG
    GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG
    GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG
    ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC
    ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT
    CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG
    ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT
    GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC
    TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC
    CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT
    CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG
    GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA
    GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA
    ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG
    ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA
    TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA
    AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT
    TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG
    AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA
    GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA
    GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG
    GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT
    CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA
    AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA
    GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG
    TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT
    CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA
    GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA
    GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT
    TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT
    GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA
    GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA
    GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT
    GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC
    CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA
    TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG
    AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA
    AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG
    AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA
    AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA
    AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG
    AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT
    AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG
    CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA
    GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT
    TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG
    CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC
    TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC
    ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG
    CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT
    GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC
    CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12597
    Clone ID Related Accession (Same CDS sequence) XM_005197281.3 , XM_005226727.4 , XM_005226727.3
    Accession Version XM_005226727.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226727.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT
    CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG
    CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA
    GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT
    TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT
    GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT
    AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA
    GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG
    ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA
    CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG
    GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG
    GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG
    ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC
    ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT
    CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG
    ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT
    GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC
    TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC
    CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT
    CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG
    GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA
    GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA
    ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG
    ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA
    TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA
    AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT
    TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG
    AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA
    GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA
    GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG
    GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT
    CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA
    AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA
    GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG
    TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT
    CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA
    GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA
    GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT
    TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT
    GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA
    GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA
    GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT
    GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC
    CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA
    TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG
    AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA
    AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG
    AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA
    AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA
    AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG
    AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT
    AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG
    CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA
    GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT
    TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG
    CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC
    TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC
    ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG
    CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT
    GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC
    CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226784.1
    CDS280..4635
    Translation

    Target ORF information:

    RefSeq Version XM_005226727.4
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226727.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT
    CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG
    CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA
    GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT
    TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT
    GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT
    AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA
    GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG
    ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA
    CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG
    GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG
    GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG
    ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC
    ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT
    CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG
    ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT
    GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC
    TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC
    CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT
    CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG
    GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA
    GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA
    ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG
    ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA
    TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA
    AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT
    TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG
    AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA
    GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA
    GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG
    GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT
    CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA
    AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA
    GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG
    TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT
    CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA
    GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA
    GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT
    TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT
    GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA
    GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA
    GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT
    GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC
    CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA
    TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG
    AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA
    AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG
    AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA
    AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA
    AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG
    AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT
    AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG
    CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA
    GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT
    TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG
    CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC
    TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC
    ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG
    CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT
    GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC
    CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12597
    Clone ID Related Accession (Same CDS sequence) XM_005197281.3 , XM_005226727.4 , XM_005226727.3
    Accession Version XM_005226727.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4356bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104633.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT
    CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG
    CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA
    GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT
    TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT
    GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT
    AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA
    GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG
    ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA
    CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG
    GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG
    GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG
    ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC
    ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT
    CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG
    ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT
    GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC
    TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC
    CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT
    CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG
    GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA
    GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA
    ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG
    ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA
    TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA
    AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT
    TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG
    AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA
    GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA
    GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG
    GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT
    CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA
    AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA
    GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG
    TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT
    CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA
    GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA
    GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT
    TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT
    GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA
    GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA
    GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT
    GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC
    CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA
    TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG
    AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA
    AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG
    AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA
    AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA
    AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG
    AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT
    AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG
    CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA
    GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT
    TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG
    CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC
    TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC
    ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG
    CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT
    GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC
    CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226784.1
    CDS284..4639
    Translation

    Target ORF information:

    RefSeq Version XM_005226727.3
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005226727.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT
    CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG
    CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA
    GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT
    TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT
    GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT
    AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA
    GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG
    ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA
    CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG
    GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG
    GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG
    ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC
    ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT
    CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG
    ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT
    GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC
    TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC
    CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT
    CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG
    GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA
    GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA
    ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG
    ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA
    TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA
    AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT
    TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG
    AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA
    GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA
    GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG
    GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT
    CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA
    AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA
    GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG
    TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT
    CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA
    GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA
    GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT
    TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT
    GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA
    GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA
    GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT
    GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC
    CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA
    TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG
    AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA
    AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG
    AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA
    AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA
    AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG
    AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT
    AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG
    CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA
    GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT
    TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG
    CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC
    TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC
    ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG
    CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT
    GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC
    CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12598
    Clone ID Related Accession (Same CDS sequence) XM_002699063.5 , XM_002699063.4 , XM_864539.7
    Accession Version XM_002699063.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699063.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA
    GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA
    GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA
    GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA
    CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG
    GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT
    ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA
    CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG
    TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG
    ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT
    GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG
    TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG
    GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG
    AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG
    AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT
    GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT
    ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG
    TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA
    CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT
    GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG
    TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA
    AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT
    GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG
    TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG
    AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT
    CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA
    AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT
    GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG
    TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA
    AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA
    CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA
    TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT
    GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC
    TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT
    GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA
    TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC
    TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG
    GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG
    AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT
    GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA
    GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA
    AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA
    CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG
    ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA
    GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA
    CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG
    TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC
    TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC
    CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA
    CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA
    GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA
    GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA
    GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC
    AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA
    GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA
    AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT
    GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG
    ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG
    CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT
    GATTATGTCT GTAACAGTGA ACAGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699109.1
    CDS280..4569
    Translation

    Target ORF information:

    RefSeq Version XM_002699063.5
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699063.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA
    GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA
    GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA
    GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA
    CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG
    GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT
    ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA
    CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG
    TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG
    ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT
    GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG
    TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG
    GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG
    AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG
    AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT
    GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT
    ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG
    TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA
    CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT
    GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG
    TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA
    AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT
    GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG
    TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG
    AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT
    CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA
    AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT
    GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG
    TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA
    AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA
    CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA
    TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT
    GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC
    TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT
    GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA
    TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC
    TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG
    GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG
    AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT
    GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA
    GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA
    AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA
    CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG
    ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA
    GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA
    CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG
    TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC
    TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC
    CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA
    CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA
    GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA
    GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA
    GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC
    AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA
    GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA
    AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT
    GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG
    ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG
    CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT
    GATTATGTCT GTAACAGTGA ACAGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12598
    Clone ID Related Accession (Same CDS sequence) XM_002699063.5 , XM_002699063.4 , XM_864539.7
    Accession Version XM_002699063.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104633.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002699063.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA
    GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA
    GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA
    GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA
    CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG
    GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT
    ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA
    CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG
    TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG
    ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT
    GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG
    TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG
    GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG
    AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG
    AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT
    GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT
    ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG
    TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA
    CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT
    GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG
    TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA
    AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT
    GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG
    TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG
    AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT
    CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA
    AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT
    GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG
    TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA
    AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA
    CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA
    TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT
    GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC
    TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT
    GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA
    TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC
    TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG
    GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG
    AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT
    GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA
    GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA
    AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA
    CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG
    ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA
    GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA
    CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG
    TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC
    TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC
    CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA
    CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA
    GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA
    GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA
    GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC
    AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA
    GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA
    AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT
    GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG
    ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG
    CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT
    GATTATGTCT GTAACAGTGA ACAGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699109.1
    CDS283..4572
    Translation

    Target ORF information:

    RefSeq Version XM_002699063.4
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699063.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA
    GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA
    GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA
    GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA
    CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG
    GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT
    ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA
    CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG
    TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG
    ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT
    GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG
    TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG
    GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG
    AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG
    AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT
    GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT
    ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG
    TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA
    CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT
    GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG
    TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA
    AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT
    GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG
    TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG
    AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT
    CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA
    AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT
    GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG
    TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA
    AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA
    CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA
    TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT
    GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC
    TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT
    GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA
    TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC
    TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG
    GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG
    AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT
    GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA
    GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA
    AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA
    CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG
    ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA
    GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA
    CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG
    TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC
    TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC
    CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA
    CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA
    GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA
    GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA
    GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC
    AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA
    GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA
    AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT
    GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG
    ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG
    CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT
    GATTATGTCT GTAACAGTGA ACAGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa12598
    Clone ID Related Accession (Same CDS sequence) XM_002699063.5 , XM_002699063.4 , XM_864539.7
    Accession Version XM_864539.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646776.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_864539.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA
    GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA
    GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA
    GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA
    CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG
    GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT
    ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA
    CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG
    TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG
    ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT
    GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG
    TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG
    GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG
    AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG
    AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT
    GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT
    ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG
    TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA
    CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT
    GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG
    TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA
    AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT
    GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG
    TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG
    AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT
    CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA
    AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT
    GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG
    TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA
    AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA
    CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA
    TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT
    GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC
    TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT
    GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA
    TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC
    TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG
    GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG
    AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT
    GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA
    GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA
    AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA
    CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG
    ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA
    GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA
    CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG
    TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC
    TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC
    CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA
    CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA
    GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA
    GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA
    GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC
    AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA
    GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA
    AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT
    GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG
    ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG
    CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT
    GATTATGTCT GTAACAGTGA ACAGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_869632.1
    CDS283..4572
    Translation

    Target ORF information:

    RefSeq Version XM_864539.7
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_864539.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA
    GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA
    GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA
    GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA
    CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG
    GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT
    ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA
    CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG
    TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG
    ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT
    GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG
    TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG
    GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG
    AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG
    AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT
    GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT
    ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG
    TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA
    CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT
    GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG
    TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA
    AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT
    GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG
    TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG
    AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT
    CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA
    AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT
    GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG
    TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA
    AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA
    CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA
    TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT
    GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC
    TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT
    GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA
    TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC
    TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG
    GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG
    AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT
    GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA
    GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA
    AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA
    CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG
    ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA
    GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA
    CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG
    TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC
    TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC
    CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA
    CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA
    GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA
    GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA
    GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC
    AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA
    GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA
    AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT
    GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG
    ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG
    CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT
    GATTATGTCT GTAACAGTGA ACAGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55069
    Clone ID Related Accession (Same CDS sequence) XM_010820856.3 , XM_010826221.2 , XM_010820856.2
    Accession Version XM_010820856.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG
    CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA
    TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA
    GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG
    AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA
    AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG
    GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC
    AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT
    TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG
    GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC
    AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA
    CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA
    CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG
    ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT
    GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA
    GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC
    TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC
    TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT
    GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA
    CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA
    TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT
    CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC
    TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC
    CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT
    GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA
    TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC
    TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG
    TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA
    GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA
    GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA
    GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA
    GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT
    GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC
    AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC
    CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC
    CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG
    ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA
    TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC
    AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG
    CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC
    TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT
    GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT
    GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT
    TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG
    AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG
    GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA
    GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA
    AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC
    CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC
    TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG
    TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC
    CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC
    TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA
    TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG
    GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG
    GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG
    GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA
    GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA
    CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT
    GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG
    ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG
    ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010819158.1
    CDS280..4701
    Translation

    Target ORF information:

    RefSeq Version XM_010820856.3
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820856.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG
    CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA
    TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA
    GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG
    AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA
    AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG
    GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC
    AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT
    TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG
    GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC
    AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA
    CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA
    CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG
    ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT
    GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA
    GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC
    TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC
    TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT
    GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA
    CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA
    TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT
    CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC
    TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC
    CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT
    GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA
    TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC
    TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG
    TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA
    GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA
    GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA
    GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA
    GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT
    GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC
    AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC
    CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC
    CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG
    ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA
    TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC
    AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG
    CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC
    TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT
    GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT
    GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT
    TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG
    AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG
    GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA
    GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA
    AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC
    CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC
    TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG
    TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC
    CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC
    TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA
    TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG
    GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG
    GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG
    GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA
    GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA
    CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT
    GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG
    ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG
    ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55069
    Clone ID Related Accession (Same CDS sequence) XM_010820856.3 , XM_010826221.2 , XM_010820856.2
    Accession Version XM_010826221.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646776.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG
    CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA
    TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA
    GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG
    AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA
    AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG
    GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC
    AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT
    TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG
    GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC
    AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA
    CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA
    CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG
    ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT
    GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA
    GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC
    TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC
    TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT
    GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA
    CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA
    TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT
    CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC
    TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC
    CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT
    GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA
    TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC
    TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG
    TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA
    GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA
    GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA
    GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA
    GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT
    GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC
    AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC
    CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC
    CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG
    ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA
    TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC
    AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG
    CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC
    TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT
    GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT
    GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT
    TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG
    AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG
    GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA
    GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA
    AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC
    CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC
    TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG
    TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC
    CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC
    TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA
    TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG
    GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG
    GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG
    GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA
    GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA
    CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT
    GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG
    ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG
    ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010824523.1
    CDS284..4705
    Translation

    Target ORF information:

    RefSeq Version XM_010826221.2
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010826221.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG
    CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA
    TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA
    GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG
    AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA
    AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG
    GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC
    AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT
    TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG
    GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC
    AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA
    CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA
    CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG
    ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT
    GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA
    GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC
    TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC
    TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT
    GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA
    CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA
    TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT
    CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC
    TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC
    CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT
    GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA
    TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC
    TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG
    TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA
    GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA
    GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA
    GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA
    GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT
    GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC
    AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC
    CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC
    CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG
    ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA
    TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC
    AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG
    CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC
    TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT
    GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT
    GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT
    TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG
    AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG
    GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA
    GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA
    AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC
    CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC
    TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG
    TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC
    CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC
    TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA
    TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG
    GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG
    GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG
    GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA
    GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA
    CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT
    GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG
    ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG
    ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55069
    Clone ID Related Accession (Same CDS sequence) XM_010820856.3 , XM_010826221.2 , XM_010820856.2
    Accession Version XM_010820856.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4422bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product remodeling and spacing factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104633.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG
    CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA
    TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA
    GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG
    AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA
    AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG
    GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC
    AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT
    TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG
    GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC
    AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA
    CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA
    CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG
    ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT
    GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA
    GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC
    TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC
    TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT
    GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA
    CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA
    TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT
    CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC
    TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC
    CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT
    GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA
    TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC
    TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG
    TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA
    GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA
    GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA
    GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA
    GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT
    GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC
    AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC
    CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC
    CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG
    ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA
    TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC
    AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG
    CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC
    TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT
    GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT
    GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT
    TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG
    AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG
    GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA
    GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA
    AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC
    CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC
    TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG
    TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC
    CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC
    TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA
    TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG
    GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG
    GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG
    GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA
    GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA
    CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT
    GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG
    ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG
    ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010819158.1
    CDS284..4705
    Translation

    Target ORF information:

    RefSeq Version XM_010820856.2
    Organism Bos taurus(cattle)
    Definition Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
    CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT
    GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC
    GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA
    TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC
    ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA
    GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT
    ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC
    CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA
    GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG
    ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG
    GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA
    ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG
    CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA
    TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA
    GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG
    AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA
    AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG
    GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC
    AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT
    TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG
    GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC
    AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA
    CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA
    CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG
    ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT
    GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA
    GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC
    TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC
    TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT
    GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA
    CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA
    TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT
    CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC
    TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC
    CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT
    GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA
    TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC
    TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG
    TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA
    GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA
    GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA
    GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA
    GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT
    GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC
    AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC
    CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC
    CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG
    ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA
    TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC
    AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG
    CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC
    TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT
    GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT
    GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT
    TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG
    AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG
    GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA
    GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA
    AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC
    CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC
    TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG
    TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC
    CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC
    TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA
    TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG
    GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG
    GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG
    GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA
    GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA
    CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT
    GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG
    ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG
    ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.