TTBK2 cDNA ORF clone, Bos taurus(cattle)

The following TTBK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTBK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa23175 NM_001206071.1
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_024997791.1
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_010809199.3
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_015473121.1
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_024997792.1
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_024997793.1
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_015473122.2
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_010809200.3
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa55044 XM_010809201.3
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa55045 XM_010809202.3
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa55045 XM_024997795.1
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa55045 XM_024997794.1
Latest version!
Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa23175 XM_015473122.1 Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa23175 XM_010809199.2 Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa55044 XM_010809201.2 Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa55045 XM_010809202.2 Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23175 XM_010809200.2 Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version NM_001206071.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000010.1. On Apr 20, 2011 this sequence version replaced XM_615092.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR6764281.11303.1, SRR5571260.46403.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
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    121
    181
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193000.1
    CDS1..3732
    Translation

    Target ORF information:

    RefSeq Version NM_001206071.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206071.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_015473122.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328608.1
    CDS631..4362
    Translation

    Target ORF information:

    RefSeq Version XM_015473122.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473122.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_010809200.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809200.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807502.1
    CDS475..4206
    Translation

    Target ORF information:

    RefSeq Version XM_010809200.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809200.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_024997791.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853559.1
    CDS492..4223
    Translation

    Target ORF information:

    RefSeq Version XM_024997791.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_010809199.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807501.1
    CDS607..4338
    Translation

    Target ORF information:

    RefSeq Version XM_010809199.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809199.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_015473122.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473122.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328608.1
    CDS646..4377
    Translation

    Target ORF information:

    RefSeq Version XM_015473122.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473122.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_010809199.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807501.1
    CDS592..4323
    Translation

    Target ORF information:

    RefSeq Version XM_010809199.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809199.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_024997793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853561.1
    CDS597..4328
    Translation

    Target ORF information:

    RefSeq Version XM_024997793.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_024997792.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853560.1
    CDS656..4387
    Translation

    Target ORF information:

    RefSeq Version XM_024997792.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_015473121.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328607.1
    CDS2231..5962
    Translation

    Target ORF information:

    RefSeq Version XM_015473121.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473121.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23175
    Clone ID Related Accession (Same CDS sequence) XM_015473122.1 , XM_010809200.3 , NM_001206071.1 , XM_024997791.1 , XM_010809199.3 , XM_015473122.2 , XM_010809199.2 , XM_024997793.1 , XM_024997792.1 , XM_015473121.1 , XM_010809200.2
    Accession Version XM_010809200.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807502.1
    CDS460..4191
    Translation

    Target ORF information:

    RefSeq Version XM_010809200.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGTCAAACTT TGCCATGGGT CGCTTTCCTA GTACGTGTCG GAAATGTTAT
    ATGCTTGATT TTGGCTTGGC TCGACAGTTT ACCAATTCCT GTGGTGACGT CAGACCACCT
    CGAGCTGTGG CAGGCTTTCG AGGGACAGTT CGTTATGCAT CAATCAATGC TCATCGGAAC
    AGGGAAATGG GAAGACATGA TGACCTTTGG TCCTTGTTCT ATATGTTGGT GGAATTTGTG
    GTTGGTCAGC TCCCTTGGAG AAAAATAAAG GACAAGGAAC AAGTGGGCTC TATTAAGGAG
    AGGTATGACC ACAGGCTTAT GTTGAAACAT CTCCCTCCAG AATTCAGCAT CTTTCTGGAG
    CACATTTCCT CTTTGGATTA TTTTACAAAA CCAGACTACC AGCTTCTTAC ATCCGTGTTT
    GACAACAGCA TCAAGGCCTT TGGAGTAATT GAAAGTGATC CTTTTGACTG GGAGAAGACT
    GGAACTGATG GCTCCCTCAC CACAACCACT ACTTCCGCCA CTCCTCAGCT GCACACCCGT
    TTGACCCCTG CGGCCATTGG AATTGCCAAC GCCACTCCCA TCCCAGGAGA CATGCTTCGA
    GAAAATACAG ATGAGGTGTT TCCAGATGAA CAGCTTAGTG ATGGAGAAAA TGGCATCCCT
    GTTGGTGTGT CACCAAATAA AGTACCCGGG TCCCTGGGAC ACCCTCATCC CCAGGAGAAG
    GATGTCTGGG AAGAGATGGA TACCAACAGG AATAAGATAA AGCTTGGGAT TTGTAAGGCT
    GCTACTGAAG AGGAAAACAG CCATGGTCAA GCAAATGGTA TTCTGAATGC TCCAAGCCTT
    GGTTCCCCAA TTCGTGTCCG CTCAGAGATT ACTCAGCCAG ACAGAGATGT TCCGTTGGTG
    CGAAAGTTAC GTTCTATCCA CAGCTTTGAG CTGGAGAAAC GCCTGGCGTT GGAACCAAAG
    CCAGATACTG ACAAGTTTCT TGAGACCTGC TTGGAGAAAA TGCAGAAAGA TCCCAGTGCA
    GGAAAAGAAT CTACTCTCCC TGCTCTGCTT CATAAGCCTT GTGTTCCAGC TGTGTCCCGT
    GCAGACCACA TCTGGCACTA TGATGAAGAA TATCTTCCGG ATGCTTCCAA GCCTGCCTCG
    GTCAACACTC CTGAGCAGGC AGACGGTGGT GGCAGCAATG GATTTGTAGC CGTTAACTTG
    AGCTCCTGCA AGCAGGAGGT TGATTCCAAA GAATGGGTGA TTGTGGACAA GGAACAGGAC
    CTTCAGGACT TTAGGACAAA TGAGGCTTTA GGCCCTAAAA CAACTGGAAG CCCTTCTGAT
    GAGGAGCCCG AAGTCCTCCA AGTCTTAGAG GAGTCGCCTC AAGATGAAAA GCTCCAACTG
    GGTCCTTGGG CAGAAAATGA TCATTTAAAG AAGGAAACCT CAGGTGTGGT CTTTGTGCTT
    TCTGGGGAAT GCCCTGCCAC GGCTGCTTCA GAGCAATATA TAGATAGGCT AGAATTCCAG
    GCTGGAGCTG CTAGTCAGTT TATCACAGTG ACACCCACAA GTCCAATGGA GGCACAAGCG
    GAAGGACCCC TTACAGCGAT TACAATTCCA AGACCTTCCG TGGCATCTAC ACAGTCAACT
    TCAGGAAGTT TTCATTATGG TCAGCAGCCA GAAAAGAAAG ACCTTCATAC CATTGAGCCC
    ACTGTGGAAC TTTACTCTCC AAGGGAGAAC TTCTCTGGCT TGGTCGTGAT AGAGGGCGAA
    CCTCCTAGTG GAGGAAGCAG AACAGACTTG GGGCTTCAGA TAGATCATAT TGGTCATGAC
    ATGTTACCCA ATATTAGAGA GTATGACAGA TCCCAAGACG TGGGACCAAA AGACCTTCCT
    GACCATAACA GACTGGCTAT GAGAGAATTT GAGGGCCTCC CTAGGGGATT GGAAGAGAAA
    AGCATTGCTT TAGGGTCAGA TAATGAAGAT GAGAAATTAA GTAAAGGGCA GCATTATATT
    GAGATCTCCC CTCTCCCAGG AGACTTGGTA ACAATGGAAA GGGATCACTC AGCTACTACT
    GAACCTCTTG ATGTGACAAA GATGCAGACT TTTAGTGTGG TGCCAAATCA AGACAAAAAT
    CATGAGATAA TGAAGCTTCT GGCAGTTGGA TCTTCAGAAG TATCTCCACG AGTTATTGAC
    CCATATGTTG AAGAGCAGAT AGGCCAGGTG GCAGCAATGC AAAAAAGTAA GATATCTGAG
    GATGACATCA AGGGTGAAGA CTTGCCCAAT CATTCTGGAG ACCTCTCTAC TTTTTTGCAC
    CAAGAGGGTA AGAGAGAGAA AATTGCCCCT AGAAAAGGAG AGCTATTTAA TCCTGTTTCA
    GAGAGTGAAC ACTGTCCCCC ATCCCGGAAG GATGTGGTTA GGTCATCCTT TGTAACTAGA
    CACAGCCGAA TCCCTGTTCT AGCACAAGAG ATAGACTCAA CTTTTGACTC ATCCTCTCCA
    GTCTCTGCAA AAGAAAAGCT CCTCCAAAAG AAAGCTTGCC AGCCAGACCT CGTCAAGCTC
    CTGGTGGAGA AAAGGCAACT CAGGTCTCTC CTTGGGGACC TCTCAAGTGC CTCTGATAAA
    CTGCTAGAGG AGAGATTAGC CACTGTTCCT GCTCCCTTTT CCGAGGAGGA AGTCTTCACC
    CCCTTTTCCA GACTGGCTGC AGACTCACAC CTGAGCAGGT CAGCCGAAGA CAACTTTCTG
    GCACCCATCA TCTCCCAGTC CAGAAAGAGC AAAATTCCAA GACCAGTTTC ATGGGTCAAC
    ACAGATCAAG TCAATAGCTC AACTCCATCT CAGTTCTTGC CTCGGCCACC ACCCGGAAAA
    CCACCCATGA GGCCTGGAGT AGAAGCCAGG CTCCGCAGAT ATAAAGTCCT AGGGAGTAGC
    AATTCTGACT CAGACCTTTT CTCCCGCCTG GCCCAAATTC TTCAAAATGG ATCTCAGAAG
    CCCCGGAGCA CTACGCAGTG CAAGAGTCCA GGAACCCCTC ACAATCCAAA AACACCACCC
    AAGAGTCCAG TTGTCCCTCG CAGGAGTCCC AGTGCCTCTC CTCGAAGCTC TTCCTTGCCT
    CGCACATCCA GTTCCTCACC ATCTCGGGCT GGACGGCCCC ACCATGACCA GAGGAGTTCA
    TCCCCACATC TGGGGAGAAG CAAGTCACCT CCCAGCCACT CGGGATCTTC CTCCTCCAGG
    AGGTCCTGCC AACAGGAGCA TTGCAAACCC AGCAAGAATG GCCTGAAAGG ATCCGGCAGC
    TTCCACCACC ACTCAGCCAG CACTAAAGCC CCCCCAGGGA AGAGTAAGTC AGCCAGTAAA
    CTCAGCAGAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55044
    Clone ID Related Accession (Same CDS sequence) XM_010809201.2 , XM_010809201.3
    Accession Version XM_010809201.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGCCTCGAGC TGTGGCAGGC TTTCGAGGGA CAGTTCGTTA TGCATCAATC
    AATGCTCATC GGAACAGGGA AATGGGAAGA CATGATGACC TTTGGTCCTT GTTCTATATG
    TTGGTGGAAT TTGTGGTTGG TCAGCTCCCT TGGAGAAAAA TAAAGGACAA GGAACAAGTG
    GGCTCTATTA AGGAGAGGTA TGACCACAGG CTTATGTTGA AACATCTCCC TCCAGAATTC
    AGCATCTTTC TGGAGCACAT TTCCTCTTTG GATTATTTTA CAAAACCAGA CTACCAGCTT
    CTTACATCCG TGTTTGACAA CAGCATCAAG GCCTTTGGAG TAATTGAAAG TGATCCTTTT
    GACTGGGAGA AGACTGGAAC TGATGGCTCC CTCACCACAA CCACTACTTC CGCCACTCCT
    CAGCTGCACA CCCGTTTGAC CCCTGCGGCC ATTGGAATTG CCAACGCCAC TCCCATCCCA
    GGAGACATGC TTCGAGAAAA TACAGATGAG GTGTTTCCAG ATGAACAGCT TAGTGATGGA
    GAAAATGGCA TCCCTGTTGG TGTGTCACCA AATAAAGTAC CCGGGTCCCT GGGACACCCT
    CATCCCCAGG AGAAGGATGT CTGGGAAGAG ATGGATACCA ACAGGAATAA GATAAAGCTT
    GGGATTTGTA AGGCTGCTAC TGAAGAGGAA AACAGCCATG GTCAAGCAAA TGGTATTCTG
    AATGCTCCAA GCCTTGGTTC CCCAATTCGT GTCCGCTCAG AGATTACTCA GCCAGACAGA
    GATGTTCCGT TGGTGCGAAA GTTACGTTCT ATCCACAGCT TTGAGCTGGA GAAACGCCTG
    GCGTTGGAAC CAAAGCCAGA TACTGACAAG TTTCTTGAGA CCTGCTTGGA GAAAATGCAG
    AAAGATCCCA GTGCAGGAAA AGAATCTACT CTCCCTGCTC TGCTTCATAA GCCTTGTGTT
    CCAGCTGTGT CCCGTGCAGA CCACATCTGG CACTATGATG AAGAATATCT TCCGGATGCT
    TCCAAGCCTG CCTCGGTCAA CACTCCTGAG CAGGCAGACG GTGGTGGCAG CAATGGATTT
    GTAGCCGTTA ACTTGAGCTC CTGCAAGCAG GAGGTTGATT CCAAAGAATG GGTGATTGTG
    GACAAGGAAC AGGACCTTCA GGACTTTAGG ACAAATGAGG CTTTAGGCCC TAAAACAACT
    GGAAGCCCTT CTGATGAGGA GCCCGAAGTC CTCCAAGTCT TAGAGGAGTC GCCTCAAGAT
    GAAAAGCTCC AACTGGGTCC TTGGGCAGAA AATGATCATT TAAAGAAGGA AACCTCAGGT
    GTGGTCTTTG TGCTTTCTGG GGAATGCCCT GCCACGGCTG CTTCAGAGCA ATATATAGAT
    AGGCTAGAAT TCCAGGCTGG AGCTGCTAGT CAGTTTATCA CAGTGACACC CACAAGTCCA
    ATGGAGGCAC AAGCGGAAGG ACCCCTTACA GCGATTACAA TTCCAAGACC TTCCGTGGCA
    TCTACACAGT CAACTTCAGG AAGTTTTCAT TATGGTCAGC AGCCAGAAAA GAAAGACCTT
    CATACCATTG AGCCCACTGT GGAACTTTAC TCTCCAAGGG AGAACTTCTC TGGCTTGGTC
    GTGATAGAGG GCGAACCTCC TAGTGGAGGA AGCAGAACAG ACTTGGGGCT TCAGATAGAT
    CATATTGGTC ATGACATGTT ACCCAATATT AGAGAGTATG ACAGATCCCA AGACGTGGGA
    CCAAAAGACC TTCCTGACCA TAACAGACTG GCTATGAGAG AATTTGAGGG CCTCCCTAGG
    GGATTGGAAG AGAAAAGCAT TGCTTTAGGG TCAGATAATG AAGATGAGAA ATTAAGTAAA
    GGGCAGCATT ATATTGAGAT CTCCCCTCTC CCAGGAGACT TGGTAACAAT GGAAAGGGAT
    CACTCAGCTA CTACTGAACC TCTTGATGTG ACAAAGATGC AGACTTTTAG TGTGGTGCCA
    AATCAAGACA AAAATCATGA GATAATGAAG CTTCTGGCAG TTGGATCTTC AGAAGTATCT
    CCACGAGTTA TTGACCCATA TGTTGAAGAG CAGATAGGCC AGGTGGCAGC AATGCAAAAA
    AGTAAGATAT CTGAGGATGA CATCAAGGGT GAAGACTTGC CCAATCATTC TGGAGACCTC
    TCTACTTTTT TGCACCAAGA GGGTAAGAGA GAGAAAATTG CCCCTAGAAA AGGAGAGCTA
    TTTAATCCTG TTTCAGAGAG TGAACACTGT CCCCCATCCC GGAAGGATGT GGTTAGGTCA
    TCCTTTGTAA CTAGACACAG CCGAATCCCT GTTCTAGCAC AAGAGATAGA CTCAACTTTT
    GACTCATCCT CTCCAGTCTC TGCAAAAGAA AAGCTCCTCC AAAAGAAAGC TTGCCAGCCA
    GACCTCGTCA AGCTCCTGGT GGAGAAAAGG CAACTCAGGT CTCTCCTTGG GGACCTCTCA
    AGTGCCTCTG ATAAACTGCT AGAGGAGAGA TTAGCCACTG TTCCTGCTCC CTTTTCCGAG
    GAGGAAGTCT TCACCCCCTT TTCCAGACTG GCTGCAGACT CACACCTGAG CAGGTCAGCC
    GAAGACAACT TTCTGGCACC CATCATCTCC CAGTCCAGAA AGAGCAAAAT TCCAAGACCA
    GTTTCATGGG TCAACACAGA TCAAGTCAAT AGCTCAACTC CATCTCAGTT CTTGCCTCGG
    CCACCACCCG GAAAACCACC CATGAGGCCT GGAGTAGAAG CCAGGCTCCG CAGATATAAA
    GTCCTAGGGA GTAGCAATTC TGACTCAGAC CTTTTCTCCC GCCTGGCCCA AATTCTTCAA
    AATGGATCTC AGAAGCCCCG GAGCACTACG CAGTGCAAGA GTCCAGGAAC CCCTCACAAT
    CCAAAAACAC CACCCAAGAG TCCAGTTGTC CCTCGCAGGA GTCCCAGTGC CTCTCCTCGA
    AGCTCTTCCT TGCCTCGCAC ATCCAGTTCC TCACCATCTC GGGCTGGACG GCCCCACCAT
    GACCAGAGGA GTTCATCCCC ACATCTGGGG AGAAGCAAGT CACCTCCCAG CCACTCGGGA
    TCTTCCTCCT CCAGGAGGTC CTGCCAACAG GAGCATTGCA AACCCAGCAA GAATGGCCTG
    AAAGGATCCG GCAGCTTCCA CCACCACTCA GCCAGCACTA AAGCCCCCCC AGGGAAGAGT
    AAGTCAGCCA GTAAACTCAG CAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807503.1
    CDS2597..6223
    Translation

    Target ORF information:

    RefSeq Version XM_010809201.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809201.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGCCTCGAGC TGTGGCAGGC TTTCGAGGGA CAGTTCGTTA TGCATCAATC
    AATGCTCATC GGAACAGGGA AATGGGAAGA CATGATGACC TTTGGTCCTT GTTCTATATG
    TTGGTGGAAT TTGTGGTTGG TCAGCTCCCT TGGAGAAAAA TAAAGGACAA GGAACAAGTG
    GGCTCTATTA AGGAGAGGTA TGACCACAGG CTTATGTTGA AACATCTCCC TCCAGAATTC
    AGCATCTTTC TGGAGCACAT TTCCTCTTTG GATTATTTTA CAAAACCAGA CTACCAGCTT
    CTTACATCCG TGTTTGACAA CAGCATCAAG GCCTTTGGAG TAATTGAAAG TGATCCTTTT
    GACTGGGAGA AGACTGGAAC TGATGGCTCC CTCACCACAA CCACTACTTC CGCCACTCCT
    CAGCTGCACA CCCGTTTGAC CCCTGCGGCC ATTGGAATTG CCAACGCCAC TCCCATCCCA
    GGAGACATGC TTCGAGAAAA TACAGATGAG GTGTTTCCAG ATGAACAGCT TAGTGATGGA
    GAAAATGGCA TCCCTGTTGG TGTGTCACCA AATAAAGTAC CCGGGTCCCT GGGACACCCT
    CATCCCCAGG AGAAGGATGT CTGGGAAGAG ATGGATACCA ACAGGAATAA GATAAAGCTT
    GGGATTTGTA AGGCTGCTAC TGAAGAGGAA AACAGCCATG GTCAAGCAAA TGGTATTCTG
    AATGCTCCAA GCCTTGGTTC CCCAATTCGT GTCCGCTCAG AGATTACTCA GCCAGACAGA
    GATGTTCCGT TGGTGCGAAA GTTACGTTCT ATCCACAGCT TTGAGCTGGA GAAACGCCTG
    GCGTTGGAAC CAAAGCCAGA TACTGACAAG TTTCTTGAGA CCTGCTTGGA GAAAATGCAG
    AAAGATCCCA GTGCAGGAAA AGAATCTACT CTCCCTGCTC TGCTTCATAA GCCTTGTGTT
    CCAGCTGTGT CCCGTGCAGA CCACATCTGG CACTATGATG AAGAATATCT TCCGGATGCT
    TCCAAGCCTG CCTCGGTCAA CACTCCTGAG CAGGCAGACG GTGGTGGCAG CAATGGATTT
    GTAGCCGTTA ACTTGAGCTC CTGCAAGCAG GAGGTTGATT CCAAAGAATG GGTGATTGTG
    GACAAGGAAC AGGACCTTCA GGACTTTAGG ACAAATGAGG CTTTAGGCCC TAAAACAACT
    GGAAGCCCTT CTGATGAGGA GCCCGAAGTC CTCCAAGTCT TAGAGGAGTC GCCTCAAGAT
    GAAAAGCTCC AACTGGGTCC TTGGGCAGAA AATGATCATT TAAAGAAGGA AACCTCAGGT
    GTGGTCTTTG TGCTTTCTGG GGAATGCCCT GCCACGGCTG CTTCAGAGCA ATATATAGAT
    AGGCTAGAAT TCCAGGCTGG AGCTGCTAGT CAGTTTATCA CAGTGACACC CACAAGTCCA
    ATGGAGGCAC AAGCGGAAGG ACCCCTTACA GCGATTACAA TTCCAAGACC TTCCGTGGCA
    TCTACACAGT CAACTTCAGG AAGTTTTCAT TATGGTCAGC AGCCAGAAAA GAAAGACCTT
    CATACCATTG AGCCCACTGT GGAACTTTAC TCTCCAAGGG AGAACTTCTC TGGCTTGGTC
    GTGATAGAGG GCGAACCTCC TAGTGGAGGA AGCAGAACAG ACTTGGGGCT TCAGATAGAT
    CATATTGGTC ATGACATGTT ACCCAATATT AGAGAGTATG ACAGATCCCA AGACGTGGGA
    CCAAAAGACC TTCCTGACCA TAACAGACTG GCTATGAGAG AATTTGAGGG CCTCCCTAGG
    GGATTGGAAG AGAAAAGCAT TGCTTTAGGG TCAGATAATG AAGATGAGAA ATTAAGTAAA
    GGGCAGCATT ATATTGAGAT CTCCCCTCTC CCAGGAGACT TGGTAACAAT GGAAAGGGAT
    CACTCAGCTA CTACTGAACC TCTTGATGTG ACAAAGATGC AGACTTTTAG TGTGGTGCCA
    AATCAAGACA AAAATCATGA GATAATGAAG CTTCTGGCAG TTGGATCTTC AGAAGTATCT
    CCACGAGTTA TTGACCCATA TGTTGAAGAG CAGATAGGCC AGGTGGCAGC AATGCAAAAA
    AGTAAGATAT CTGAGGATGA CATCAAGGGT GAAGACTTGC CCAATCATTC TGGAGACCTC
    TCTACTTTTT TGCACCAAGA GGGTAAGAGA GAGAAAATTG CCCCTAGAAA AGGAGAGCTA
    TTTAATCCTG TTTCAGAGAG TGAACACTGT CCCCCATCCC GGAAGGATGT GGTTAGGTCA
    TCCTTTGTAA CTAGACACAG CCGAATCCCT GTTCTAGCAC AAGAGATAGA CTCAACTTTT
    GACTCATCCT CTCCAGTCTC TGCAAAAGAA AAGCTCCTCC AAAAGAAAGC TTGCCAGCCA
    GACCTCGTCA AGCTCCTGGT GGAGAAAAGG CAACTCAGGT CTCTCCTTGG GGACCTCTCA
    AGTGCCTCTG ATAAACTGCT AGAGGAGAGA TTAGCCACTG TTCCTGCTCC CTTTTCCGAG
    GAGGAAGTCT TCACCCCCTT TTCCAGACTG GCTGCAGACT CACACCTGAG CAGGTCAGCC
    GAAGACAACT TTCTGGCACC CATCATCTCC CAGTCCAGAA AGAGCAAAAT TCCAAGACCA
    GTTTCATGGG TCAACACAGA TCAAGTCAAT AGCTCAACTC CATCTCAGTT CTTGCCTCGG
    CCACCACCCG GAAAACCACC CATGAGGCCT GGAGTAGAAG CCAGGCTCCG CAGATATAAA
    GTCCTAGGGA GTAGCAATTC TGACTCAGAC CTTTTCTCCC GCCTGGCCCA AATTCTTCAA
    AATGGATCTC AGAAGCCCCG GAGCACTACG CAGTGCAAGA GTCCAGGAAC CCCTCACAAT
    CCAAAAACAC CACCCAAGAG TCCAGTTGTC CCTCGCAGGA GTCCCAGTGC CTCTCCTCGA
    AGCTCTTCCT TGCCTCGCAC ATCCAGTTCC TCACCATCTC GGGCTGGACG GCCCCACCAT
    GACCAGAGGA GTTCATCCCC ACATCTGGGG AGAAGCAAGT CACCTCCCAG CCACTCGGGA
    TCTTCCTCCT CCAGGAGGTC CTGCCAACAG GAGCATTGCA AACCCAGCAA GAATGGCCTG
    AAAGGATCCG GCAGCTTCCA CCACCACTCA GCCAGCACTA AAGCCCCCCC AGGGAAGAGT
    AAGTCAGCCA GTAAACTCAG CAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55044
    Clone ID Related Accession (Same CDS sequence) XM_010809201.2 , XM_010809201.3
    Accession Version XM_010809201.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809201.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGCCTCGAGC TGTGGCAGGC TTTCGAGGGA CAGTTCGTTA TGCATCAATC
    AATGCTCATC GGAACAGGGA AATGGGAAGA CATGATGACC TTTGGTCCTT GTTCTATATG
    TTGGTGGAAT TTGTGGTTGG TCAGCTCCCT TGGAGAAAAA TAAAGGACAA GGAACAAGTG
    GGCTCTATTA AGGAGAGGTA TGACCACAGG CTTATGTTGA AACATCTCCC TCCAGAATTC
    AGCATCTTTC TGGAGCACAT TTCCTCTTTG GATTATTTTA CAAAACCAGA CTACCAGCTT
    CTTACATCCG TGTTTGACAA CAGCATCAAG GCCTTTGGAG TAATTGAAAG TGATCCTTTT
    GACTGGGAGA AGACTGGAAC TGATGGCTCC CTCACCACAA CCACTACTTC CGCCACTCCT
    CAGCTGCACA CCCGTTTGAC CCCTGCGGCC ATTGGAATTG CCAACGCCAC TCCCATCCCA
    GGAGACATGC TTCGAGAAAA TACAGATGAG GTGTTTCCAG ATGAACAGCT TAGTGATGGA
    GAAAATGGCA TCCCTGTTGG TGTGTCACCA AATAAAGTAC CCGGGTCCCT GGGACACCCT
    CATCCCCAGG AGAAGGATGT CTGGGAAGAG ATGGATACCA ACAGGAATAA GATAAAGCTT
    GGGATTTGTA AGGCTGCTAC TGAAGAGGAA AACAGCCATG GTCAAGCAAA TGGTATTCTG
    AATGCTCCAA GCCTTGGTTC CCCAATTCGT GTCCGCTCAG AGATTACTCA GCCAGACAGA
    GATGTTCCGT TGGTGCGAAA GTTACGTTCT ATCCACAGCT TTGAGCTGGA GAAACGCCTG
    GCGTTGGAAC CAAAGCCAGA TACTGACAAG TTTCTTGAGA CCTGCTTGGA GAAAATGCAG
    AAAGATCCCA GTGCAGGAAA AGAATCTACT CTCCCTGCTC TGCTTCATAA GCCTTGTGTT
    CCAGCTGTGT CCCGTGCAGA CCACATCTGG CACTATGATG AAGAATATCT TCCGGATGCT
    TCCAAGCCTG CCTCGGTCAA CACTCCTGAG CAGGCAGACG GTGGTGGCAG CAATGGATTT
    GTAGCCGTTA ACTTGAGCTC CTGCAAGCAG GAGGTTGATT CCAAAGAATG GGTGATTGTG
    GACAAGGAAC AGGACCTTCA GGACTTTAGG ACAAATGAGG CTTTAGGCCC TAAAACAACT
    GGAAGCCCTT CTGATGAGGA GCCCGAAGTC CTCCAAGTCT TAGAGGAGTC GCCTCAAGAT
    GAAAAGCTCC AACTGGGTCC TTGGGCAGAA AATGATCATT TAAAGAAGGA AACCTCAGGT
    GTGGTCTTTG TGCTTTCTGG GGAATGCCCT GCCACGGCTG CTTCAGAGCA ATATATAGAT
    AGGCTAGAAT TCCAGGCTGG AGCTGCTAGT CAGTTTATCA CAGTGACACC CACAAGTCCA
    ATGGAGGCAC AAGCGGAAGG ACCCCTTACA GCGATTACAA TTCCAAGACC TTCCGTGGCA
    TCTACACAGT CAACTTCAGG AAGTTTTCAT TATGGTCAGC AGCCAGAAAA GAAAGACCTT
    CATACCATTG AGCCCACTGT GGAACTTTAC TCTCCAAGGG AGAACTTCTC TGGCTTGGTC
    GTGATAGAGG GCGAACCTCC TAGTGGAGGA AGCAGAACAG ACTTGGGGCT TCAGATAGAT
    CATATTGGTC ATGACATGTT ACCCAATATT AGAGAGTATG ACAGATCCCA AGACGTGGGA
    CCAAAAGACC TTCCTGACCA TAACAGACTG GCTATGAGAG AATTTGAGGG CCTCCCTAGG
    GGATTGGAAG AGAAAAGCAT TGCTTTAGGG TCAGATAATG AAGATGAGAA ATTAAGTAAA
    GGGCAGCATT ATATTGAGAT CTCCCCTCTC CCAGGAGACT TGGTAACAAT GGAAAGGGAT
    CACTCAGCTA CTACTGAACC TCTTGATGTG ACAAAGATGC AGACTTTTAG TGTGGTGCCA
    AATCAAGACA AAAATCATGA GATAATGAAG CTTCTGGCAG TTGGATCTTC AGAAGTATCT
    CCACGAGTTA TTGACCCATA TGTTGAAGAG CAGATAGGCC AGGTGGCAGC AATGCAAAAA
    AGTAAGATAT CTGAGGATGA CATCAAGGGT GAAGACTTGC CCAATCATTC TGGAGACCTC
    TCTACTTTTT TGCACCAAGA GGGTAAGAGA GAGAAAATTG CCCCTAGAAA AGGAGAGCTA
    TTTAATCCTG TTTCAGAGAG TGAACACTGT CCCCCATCCC GGAAGGATGT GGTTAGGTCA
    TCCTTTGTAA CTAGACACAG CCGAATCCCT GTTCTAGCAC AAGAGATAGA CTCAACTTTT
    GACTCATCCT CTCCAGTCTC TGCAAAAGAA AAGCTCCTCC AAAAGAAAGC TTGCCAGCCA
    GACCTCGTCA AGCTCCTGGT GGAGAAAAGG CAACTCAGGT CTCTCCTTGG GGACCTCTCA
    AGTGCCTCTG ATAAACTGCT AGAGGAGAGA TTAGCCACTG TTCCTGCTCC CTTTTCCGAG
    GAGGAAGTCT TCACCCCCTT TTCCAGACTG GCTGCAGACT CACACCTGAG CAGGTCAGCC
    GAAGACAACT TTCTGGCACC CATCATCTCC CAGTCCAGAA AGAGCAAAAT TCCAAGACCA
    GTTTCATGGG TCAACACAGA TCAAGTCAAT AGCTCAACTC CATCTCAGTT CTTGCCTCGG
    CCACCACCCG GAAAACCACC CATGAGGCCT GGAGTAGAAG CCAGGCTCCG CAGATATAAA
    GTCCTAGGGA GTAGCAATTC TGACTCAGAC CTTTTCTCCC GCCTGGCCCA AATTCTTCAA
    AATGGATCTC AGAAGCCCCG GAGCACTACG CAGTGCAAGA GTCCAGGAAC CCCTCACAAT
    CCAAAAACAC CACCCAAGAG TCCAGTTGTC CCTCGCAGGA GTCCCAGTGC CTCTCCTCGA
    AGCTCTTCCT TGCCTCGCAC ATCCAGTTCC TCACCATCTC GGGCTGGACG GCCCCACCAT
    GACCAGAGGA GTTCATCCCC ACATCTGGGG AGAAGCAAGT CACCTCCCAG CCACTCGGGA
    TCTTCCTCCT CCAGGAGGTC CTGCCAACAG GAGCATTGCA AACCCAGCAA GAATGGCCTG
    AAAGGATCCG GCAGCTTCCA CCACCACTCA GCCAGCACTA AAGCCCCCCC AGGGAAGAGT
    AAGTCAGCCA GTAAACTCAG CAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807503.1
    CDS494..4120
    Translation

    Target ORF information:

    RefSeq Version XM_010809201.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809201.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAGTGGAG GAGGAGAGCA GCCGGATATC CTCAGTGTTG GAATCCTGGT CAAAGAAAGA 
    TGGAAAGTAT TGAGGAAGAT TGGGGGTGGG GGCTTTGGAG AAATTTACGA TGCCTTGGAC
    ATGCTCACCA GGGAAAATGT TGCACTGAAG GTGGAATCAG CTCAACAACC AAAGCAAGTT
    CTGAAAATGG AGGTTGCTGT ATTGAAAAAA CTACAAGGGA AAGACCATGT TTGTAGATTT
    ATTGGTTGTG GGAGGAATGA TCGATTCAAC TACGTGGTCA TGCAGTTGCA GGGCCGGAAT
    CTGGCAGATC TACGCCGGAG CCAGTCCCGG GGCACATTCA CCATTAGCAC TACTCTTCGC
    TTGGGTAGAC AGATTCTGGA GTCTATTGAG AGCATCCATT CTGTGGGATT CTTGCACCGA
    GACATCAAAC CGCCTCGAGC TGTGGCAGGC TTTCGAGGGA CAGTTCGTTA TGCATCAATC
    AATGCTCATC GGAACAGGGA AATGGGAAGA CATGATGACC TTTGGTCCTT GTTCTATATG
    TTGGTGGAAT TTGTGGTTGG TCAGCTCCCT TGGAGAAAAA TAAAGGACAA GGAACAAGTG
    GGCTCTATTA AGGAGAGGTA TGACCACAGG CTTATGTTGA AACATCTCCC TCCAGAATTC
    AGCATCTTTC TGGAGCACAT TTCCTCTTTG GATTATTTTA CAAAACCAGA CTACCAGCTT
    CTTACATCCG TGTTTGACAA CAGCATCAAG GCCTTTGGAG TAATTGAAAG TGATCCTTTT
    GACTGGGAGA AGACTGGAAC TGATGGCTCC CTCACCACAA CCACTACTTC CGCCACTCCT
    CAGCTGCACA CCCGTTTGAC CCCTGCGGCC ATTGGAATTG CCAACGCCAC TCCCATCCCA
    GGAGACATGC TTCGAGAAAA TACAGATGAG GTGTTTCCAG ATGAACAGCT TAGTGATGGA
    GAAAATGGCA TCCCTGTTGG TGTGTCACCA AATAAAGTAC CCGGGTCCCT GGGACACCCT
    CATCCCCAGG AGAAGGATGT CTGGGAAGAG ATGGATACCA ACAGGAATAA GATAAAGCTT
    GGGATTTGTA AGGCTGCTAC TGAAGAGGAA AACAGCCATG GTCAAGCAAA TGGTATTCTG
    AATGCTCCAA GCCTTGGTTC CCCAATTCGT GTCCGCTCAG AGATTACTCA GCCAGACAGA
    GATGTTCCGT TGGTGCGAAA GTTACGTTCT ATCCACAGCT TTGAGCTGGA GAAACGCCTG
    GCGTTGGAAC CAAAGCCAGA TACTGACAAG TTTCTTGAGA CCTGCTTGGA GAAAATGCAG
    AAAGATCCCA GTGCAGGAAA AGAATCTACT CTCCCTGCTC TGCTTCATAA GCCTTGTGTT
    CCAGCTGTGT CCCGTGCAGA CCACATCTGG CACTATGATG AAGAATATCT TCCGGATGCT
    TCCAAGCCTG CCTCGGTCAA CACTCCTGAG CAGGCAGACG GTGGTGGCAG CAATGGATTT
    GTAGCCGTTA ACTTGAGCTC CTGCAAGCAG GAGGTTGATT CCAAAGAATG GGTGATTGTG
    GACAAGGAAC AGGACCTTCA GGACTTTAGG ACAAATGAGG CTTTAGGCCC TAAAACAACT
    GGAAGCCCTT CTGATGAGGA GCCCGAAGTC CTCCAAGTCT TAGAGGAGTC GCCTCAAGAT
    GAAAAGCTCC AACTGGGTCC TTGGGCAGAA AATGATCATT TAAAGAAGGA AACCTCAGGT
    GTGGTCTTTG TGCTTTCTGG GGAATGCCCT GCCACGGCTG CTTCAGAGCA ATATATAGAT
    AGGCTAGAAT TCCAGGCTGG AGCTGCTAGT CAGTTTATCA CAGTGACACC CACAAGTCCA
    ATGGAGGCAC AAGCGGAAGG ACCCCTTACA GCGATTACAA TTCCAAGACC TTCCGTGGCA
    TCTACACAGT CAACTTCAGG AAGTTTTCAT TATGGTCAGC AGCCAGAAAA GAAAGACCTT
    CATACCATTG AGCCCACTGT GGAACTTTAC TCTCCAAGGG AGAACTTCTC TGGCTTGGTC
    GTGATAGAGG GCGAACCTCC TAGTGGAGGA AGCAGAACAG ACTTGGGGCT TCAGATAGAT
    CATATTGGTC ATGACATGTT ACCCAATATT AGAGAGTATG ACAGATCCCA AGACGTGGGA
    CCAAAAGACC TTCCTGACCA TAACAGACTG GCTATGAGAG AATTTGAGGG CCTCCCTAGG
    GGATTGGAAG AGAAAAGCAT TGCTTTAGGG TCAGATAATG AAGATGAGAA ATTAAGTAAA
    GGGCAGCATT ATATTGAGAT CTCCCCTCTC CCAGGAGACT TGGTAACAAT GGAAAGGGAT
    CACTCAGCTA CTACTGAACC TCTTGATGTG ACAAAGATGC AGACTTTTAG TGTGGTGCCA
    AATCAAGACA AAAATCATGA GATAATGAAG CTTCTGGCAG TTGGATCTTC AGAAGTATCT
    CCACGAGTTA TTGACCCATA TGTTGAAGAG CAGATAGGCC AGGTGGCAGC AATGCAAAAA
    AGTAAGATAT CTGAGGATGA CATCAAGGGT GAAGACTTGC CCAATCATTC TGGAGACCTC
    TCTACTTTTT TGCACCAAGA GGGTAAGAGA GAGAAAATTG CCCCTAGAAA AGGAGAGCTA
    TTTAATCCTG TTTCAGAGAG TGAACACTGT CCCCCATCCC GGAAGGATGT GGTTAGGTCA
    TCCTTTGTAA CTAGACACAG CCGAATCCCT GTTCTAGCAC AAGAGATAGA CTCAACTTTT
    GACTCATCCT CTCCAGTCTC TGCAAAAGAA AAGCTCCTCC AAAAGAAAGC TTGCCAGCCA
    GACCTCGTCA AGCTCCTGGT GGAGAAAAGG CAACTCAGGT CTCTCCTTGG GGACCTCTCA
    AGTGCCTCTG ATAAACTGCT AGAGGAGAGA TTAGCCACTG TTCCTGCTCC CTTTTCCGAG
    GAGGAAGTCT TCACCCCCTT TTCCAGACTG GCTGCAGACT CACACCTGAG CAGGTCAGCC
    GAAGACAACT TTCTGGCACC CATCATCTCC CAGTCCAGAA AGAGCAAAAT TCCAAGACCA
    GTTTCATGGG TCAACACAGA TCAAGTCAAT AGCTCAACTC CATCTCAGTT CTTGCCTCGG
    CCACCACCCG GAAAACCACC CATGAGGCCT GGAGTAGAAG CCAGGCTCCG CAGATATAAA
    GTCCTAGGGA GTAGCAATTC TGACTCAGAC CTTTTCTCCC GCCTGGCCCA AATTCTTCAA
    AATGGATCTC AGAAGCCCCG GAGCACTACG CAGTGCAAGA GTCCAGGAAC CCCTCACAAT
    CCAAAAACAC CACCCAAGAG TCCAGTTGTC CCTCGCAGGA GTCCCAGTGC CTCTCCTCGA
    AGCTCTTCCT TGCCTCGCAC ATCCAGTTCC TCACCATCTC GGGCTGGACG GCCCCACCAT
    GACCAGAGGA GTTCATCCCC ACATCTGGGG AGAAGCAAGT CACCTCCCAG CCACTCGGGA
    TCTTCCTCCT CCAGGAGGTC CTGCCAACAG GAGCATTGCA AACCCAGCAA GAATGGCCTG
    AAAGGATCCG GCAGCTTCCA CCACCACTCA GCCAGCACTA AAGCCCCCCC AGGGAAGAGT
    AAGTCAGCCA GTAAACTCAG CAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55045
    Clone ID Related Accession (Same CDS sequence) XM_024997795.1 , XM_024997794.1 , XM_010809202.2 , XM_010809202.3
    Accession Version XM_024997795.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853563.1
    CDS84..3536
    Translation

    Target ORF information:

    RefSeq Version XM_024997795.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997795.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55045
    Clone ID Related Accession (Same CDS sequence) XM_024997795.1 , XM_024997794.1 , XM_010809202.2 , XM_010809202.3
    Accession Version XM_024997794.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853562.1
    CDS662..4114
    Translation

    Target ORF information:

    RefSeq Version XM_024997794.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55045
    Clone ID Related Accession (Same CDS sequence) XM_024997795.1 , XM_024997794.1 , XM_010809202.2 , XM_010809202.3
    Accession Version XM_010809202.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807504.1
    CDS281..3733
    Translation

    Target ORF information:

    RefSeq Version XM_010809202.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809202.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa55045
    Clone ID Related Accession (Same CDS sequence) XM_024997795.1 , XM_024997794.1 , XM_010809202.2 , XM_010809202.3
    Accession Version XM_010809202.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product tau-tubulin kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010809202.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010807504.1
    CDS310..3762
    Translation

    Target ORF information:

    RefSeq Version XM_010809202.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tau tubulin kinase 2 (TTBK2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010809202.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCAGTTGC AGGGCCGGAA TCTGGCAGAT CTACGCCGGA GCCAGTCCCG GGGCACATTC 
    ACCATTAGCA CTACTCTTCG CTTGGGTAGA CAGATTCTGG AGTCTATTGA GAGCATCCAT
    TCTGTGGGAT TCTTGCACCG AGACATCAAA CCGTCAAACT TTGCCATGGG TCGCTTTCCT
    AGTACGTGTC GGAAATGTTA TATGCTTGAT TTTGGCTTGG CTCGACAGTT TACCAATTCC
    TGTGGTGACG TCAGACCACC TCGAGCTGTG GCAGGCTTTC GAGGGACAGT TCGTTATGCA
    TCAATCAATG CTCATCGGAA CAGGGAAATG GGAAGACATG ATGACCTTTG GTCCTTGTTC
    TATATGTTGG TGGAATTTGT GGTTGGTCAG CTCCCTTGGA GAAAAATAAA GGACAAGGAA
    CAAGTGGGCT CTATTAAGGA GAGGTATGAC CACAGGCTTA TGTTGAAACA TCTCCCTCCA
    GAATTCAGCA TCTTTCTGGA GCACATTTCC TCTTTGGATT ATTTTACAAA ACCAGACTAC
    CAGCTTCTTA CATCCGTGTT TGACAACAGC ATCAAGGCCT TTGGAGTAAT TGAAAGTGAT
    CCTTTTGACT GGGAGAAGAC TGGAACTGAT GGCTCCCTCA CCACAACCAC TACTTCCGCC
    ACTCCTCAGC TGCACACCCG TTTGACCCCT GCGGCCATTG GAATTGCCAA CGCCACTCCC
    ATCCCAGGAG ACATGCTTCG AGAAAATACA GATGAGGTGT TTCCAGATGA ACAGCTTAGT
    GATGGAGAAA ATGGCATCCC TGTTGGTGTG TCACCAAATA AAGTACCCGG GTCCCTGGGA
    CACCCTCATC CCCAGGAGAA GGATGTCTGG GAAGAGATGG ATACCAACAG GAATAAGATA
    AAGCTTGGGA TTTGTAAGGC TGCTACTGAA GAGGAAAACA GCCATGGTCA AGCAAATGGT
    ATTCTGAATG CTCCAAGCCT TGGTTCCCCA ATTCGTGTCC GCTCAGAGAT TACTCAGCCA
    GACAGAGATG TTCCGTTGGT GCGAAAGTTA CGTTCTATCC ACAGCTTTGA GCTGGAGAAA
    CGCCTGGCGT TGGAACCAAA GCCAGATACT GACAAGTTTC TTGAGACCTG CTTGGAGAAA
    ATGCAGAAAG ATCCCAGTGC AGGAAAAGAA TCTACTCTCC CTGCTCTGCT TCATAAGCCT
    TGTGTTCCAG CTGTGTCCCG TGCAGACCAC ATCTGGCACT ATGATGAAGA ATATCTTCCG
    GATGCTTCCA AGCCTGCCTC GGTCAACACT CCTGAGCAGG CAGACGGTGG TGGCAGCAAT
    GGATTTGTAG CCGTTAACTT GAGCTCCTGC AAGCAGGAGG TTGATTCCAA AGAATGGGTG
    ATTGTGGACA AGGAACAGGA CCTTCAGGAC TTTAGGACAA ATGAGGCTTT AGGCCCTAAA
    ACAACTGGAA GCCCTTCTGA TGAGGAGCCC GAAGTCCTCC AAGTCTTAGA GGAGTCGCCT
    CAAGATGAAA AGCTCCAACT GGGTCCTTGG GCAGAAAATG ATCATTTAAA GAAGGAAACC
    TCAGGTGTGG TCTTTGTGCT TTCTGGGGAA TGCCCTGCCA CGGCTGCTTC AGAGCAATAT
    ATAGATAGGC TAGAATTCCA GGCTGGAGCT GCTAGTCAGT TTATCACAGT GACACCCACA
    AGTCCAATGG AGGCACAAGC GGAAGGACCC CTTACAGCGA TTACAATTCC AAGACCTTCC
    GTGGCATCTA CACAGTCAAC TTCAGGAAGT TTTCATTATG GTCAGCAGCC AGAAAAGAAA
    GACCTTCATA CCATTGAGCC CACTGTGGAA CTTTACTCTC CAAGGGAGAA CTTCTCTGGC
    TTGGTCGTGA TAGAGGGCGA ACCTCCTAGT GGAGGAAGCA GAACAGACTT GGGGCTTCAG
    ATAGATCATA TTGGTCATGA CATGTTACCC AATATTAGAG AGTATGACAG ATCCCAAGAC
    GTGGGACCAA AAGACCTTCC TGACCATAAC AGACTGGCTA TGAGAGAATT TGAGGGCCTC
    CCTAGGGGAT TGGAAGAGAA AAGCATTGCT TTAGGGTCAG ATAATGAAGA TGAGAAATTA
    AGTAAAGGGC AGCATTATAT TGAGATCTCC CCTCTCCCAG GAGACTTGGT AACAATGGAA
    AGGGATCACT CAGCTACTAC TGAACCTCTT GATGTGACAA AGATGCAGAC TTTTAGTGTG
    GTGCCAAATC AAGACAAAAA TCATGAGATA ATGAAGCTTC TGGCAGTTGG ATCTTCAGAA
    GTATCTCCAC GAGTTATTGA CCCATATGTT GAAGAGCAGA TAGGCCAGGT GGCAGCAATG
    CAAAAAAGTA AGATATCTGA GGATGACATC AAGGGTGAAG ACTTGCCCAA TCATTCTGGA
    GACCTCTCTA CTTTTTTGCA CCAAGAGGGT AAGAGAGAGA AAATTGCCCC TAGAAAAGGA
    GAGCTATTTA ATCCTGTTTC AGAGAGTGAA CACTGTCCCC CATCCCGGAA GGATGTGGTT
    AGGTCATCCT TTGTAACTAG ACACAGCCGA ATCCCTGTTC TAGCACAAGA GATAGACTCA
    ACTTTTGACT CATCCTCTCC AGTCTCTGCA AAAGAAAAGC TCCTCCAAAA GAAAGCTTGC
    CAGCCAGACC TCGTCAAGCT CCTGGTGGAG AAAAGGCAAC TCAGGTCTCT CCTTGGGGAC
    CTCTCAAGTG CCTCTGATAA ACTGCTAGAG GAGAGATTAG CCACTGTTCC TGCTCCCTTT
    TCCGAGGAGG AAGTCTTCAC CCCCTTTTCC AGACTGGCTG CAGACTCACA CCTGAGCAGG
    TCAGCCGAAG ACAACTTTCT GGCACCCATC ATCTCCCAGT CCAGAAAGAG CAAAATTCCA
    AGACCAGTTT CATGGGTCAA CACAGATCAA GTCAATAGCT CAACTCCATC TCAGTTCTTG
    CCTCGGCCAC CACCCGGAAA ACCACCCATG AGGCCTGGAG TAGAAGCCAG GCTCCGCAGA
    TATAAAGTCC TAGGGAGTAG CAATTCTGAC TCAGACCTTT TCTCCCGCCT GGCCCAAATT
    CTTCAAAATG GATCTCAGAA GCCCCGGAGC ACTACGCAGT GCAAGAGTCC AGGAACCCCT
    CACAATCCAA AAACACCACC CAAGAGTCCA GTTGTCCCTC GCAGGAGTCC CAGTGCCTCT
    CCTCGAAGCT CTTCCTTGCC TCGCACATCC AGTTCCTCAC CATCTCGGGC TGGACGGCCC
    CACCATGACC AGAGGAGTTC ATCCCCACAT CTGGGGAGAA GCAAGTCACC TCCCAGCCAC
    TCGGGATCTT CCTCCTCCAG GAGGTCCTGC CAACAGGAGC ATTGCAAACC CAGCAAGAAT
    GGCCTGAAAG GATCCGGCAG CTTCCACCAC CACTCAGCCA GCACTAAAGC CCCCCCAGGG
    AAGAGTAAGT CAGCCAGTAA ACTCAGCAGA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.