KDM5B cDNA ORF clone, Bos taurus(cattle)

The following KDM5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223794 XM_024976576.1
Latest version!
Bos taurus lysine demethylase 5B (KDM5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223796 XM_024976578.1
Latest version!
Bos taurus lysine demethylase 5B (KDM5B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa93014 XM_015475206.1
Latest version!
Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa93014 XM_015466901.1
Latest version!
Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa93015 XM_015475207.1
Latest version!
Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa93015 XM_015466902.1
Latest version!
Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa223795 XM_024976577.1
Latest version!
Bos taurus lysine demethylase 5B (KDM5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223794
    Clone ID Related Accession (Same CDS sequence) XM_024976576.1
    Accession Version XM_024976576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGCCC CCGGCCCGCG CCCCGTGCCG GCCCTCGGCG GTCCCGGCCC ACTGGGGGAG 
    TTCCTGCCGC CGCCCGAGTG CCCGGTCTTC GAGCCCAGCT GGGAGGAGTT CGCCGATCCC
    TTCGCCTTCA TCCACAAGAT ACGGCCCATA GCCGAGCAGA CCGGGATCTG CAAGGTGCGG
    CCGCCGCCGG ATTGGCAGCC ACCATTTGCC TGTGATGTTG ACAAACTTCA TTTTACCCCG
    CGTATCCAGA GGCTGAATGA ACTGGAGGCT CAAACACGTG TAAAATTAAA TTTCCTGGAC
    CAGATTGCAA AGTACTGGGA ATTACAGGGA AGTACTCTGA AAATTCCACA CGTGGAGAGG
    AAGATCTTGG ACTTGTTTCA GCTTAATAAG TTAGTTGCAG AGGAAGGTGG CTTTGCAGTT
    GTTTGCAAGG ATAGAAAATG GACCAAAATT GCCACCAAGA TGGGCTTTGC TCCTGGCAAA
    GCTGTGGGCT CTCATATAAG AGGACACTAC GAGCGGATTC TGAACCCCTA CAACTTATTT
    CTGTCTGGAG ACAGCTTGAG GGCCATGAAT ATTAAAATAG AACCTGAAGA GACAACAGAA
    GCCAGAACTC ATAACCTGAG ACGTCGAATG GGATGTCCAG CTCCAAAGTG TGAGAATGAC
    AAAGACGTGA AAAGCAGCGT CAAGCAAGAA CCTGTTGAAA AGAAAGAGTG TGTTACAGAA
    AGTGAGAAGG AAAAACCCAA GAGTCGAGCT AAAAAAACTA CCAATGCTGT TGACCTGTAC
    GTCTGCCTCT TGTGTGGCAG TGGCAGCGAC GAGGACCGGC TTCTCCTGTG CGATGGGTGT
    GATGACAGCT ACCACACCTT CTGCCTGATC CCGCCGCTCC ATGACGTTCC CAAGGGAGAC
    TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA TGCAGTAAGC CACAAGAAGC ATTCGGCTTT
    GAGCAGGCGG CCAGGGATTA TACTCTCCGT ACTTTTGGTG AAATGGCAGA TGCATTCAAA
    TCTGATTACT TCAACATGCC AGTCCATATG GTTCCCACGG AACTGGTAGA GAAGGAATTT
    TGGCGACTAG TGAGCACTAT TGAAGAGGAT GTCACCGTGG AATATGGAGC CGATATTGCC
    TCGAAGGAGT TCGGCAGTGG CTTCCCTGTC CGAGATGGGA AAGTCAAGCT CTCACCTGAG
    GAGGAGGAGT ATCTTGATAG TGGCTGGAAT CTGAACAACA TGCCAGTGAT GGAGCAGTCT
    GTTCTTGCTC ACATCACTGC CGACATATGC GGCATGAAGC TTCCTTGGCT GTACGTGGGA
    ATGTGCTTTT CGTCCTTCTG TTGGCACATT GAAGACCACT GGAGCTATTC AATTAACTAC
    CTGCACTGGG GTGAGCCAAA GACCTGGTAC GGCGTTCCCG GGTACGCTGC TGAGCAGCTG
    GAGACCGTGA TGAAGAAGCT GGCCCCAGAG CTCTTCATCT CCCAGCCCGA CCTTCTCCAC
    CAGCTTGTCA CCATCATGAA CCCCAACACC CTGATGACTC ACGAAGTGCC TGTTTACCGA
    ACCAATCAGT GTGCTGGGGA GTTTGTGATC ACGTTTCCAA GAGCCTACCA CAGCGGTTTC
    AATCAAGGTT TCAATTTTGC TGAGGCCGTT AACTTCTGCA CTGTTGATTG GCTGCCCTTA
    GGCCGGCAGT GCGTGGAGCA TTACCGTCTG CTCCACCGCT ACTGCGTGTT CTCCCACGAC
    GAGATGATCT GCAAGATGGC TTCCAAGGCG GATGTCCTAG ACGTTGTAGT GGCCTCAACT
    GTTCAGAAAG ACATGGCCAT CATGATTGAG GACGAGAAGG CACTCAGGGA AACTGTTCGC
    AAATTGGGAG TAATTGATTC GGAAAGAATG GATTTTGAAC TGTTGCCGGA TGATGAGCGT
    CAGTGTGTGA AATGCAAAAC CACGTGCTTC ATGTCTGCCC TCTCCTGCTG CTGCAAACCT
    GGCCTGCTTG TTTGCCTGCA TCACGTACAG GAGTTATGTT CCTGTCCTCC TTATAAATAC
    AAACTGAGGT ACAGATACAC TCTGGATGAC CTCTACCCCA TGATGAATGC ACTGAAGCTT
    CGAGCAGAGT CTTATAACGA GTGGGCCTTG AATGTGAATG AAGCTTTGGA GGCTAAGATT
    AATAAGAAGA AAAGCCTTGT CAGCTTTAAG GCTTTGATTG AAGAATCTGA GATGAAGAAA
    TTTCCAGACA ACGATCTTCT ACGTCACCTC CGCTTAGTCA CACAGGACGC GGAGAAATGC
    GCCTCTGTTG CCCAGCAGCT ACTTAATGGA AAGAGGCAGA CGAGATACCG GTCTGGTGGA
    GGGAAATCCC ACAATCAGCT GACCGTGAAT GAGCTCCGGC AGTTTGTGAC CCAGCTGCAC
    GCCCTCCCCT GTGTCCTCAG TCAGACACCG CTGCTGAAGG ATCTCTTGAA TCGTGTAGAA
    GATTTCCAAC AGCATAGCCA GAAACTGCTC TCTGAGGAGA TGCCCAGTGC TTCTGAGCTG
    CAGGACTTGC TGGATGTCAG CTTTGAATTT GATGTTGAGC TCCCCCAGCT TGCTGAGATG
    CGTGTCCGGC TGGAGCAGGC CCATTGGCTG GAGGAGGTGC AGCAGGCTTG CCTGGACCCG
    AGCTCCCTCA CCTTAGATGA TATGAGACGT CTCATAGACC TGGGAGTGGG CCTGGCCCCA
    TATTCAGCCG TGGAGAAGGC TATGGCGCGG CTTCAGGAAC TGCTCACAGT ATCAGAGCAC
    TGGGATGACA AAGCCAAGAG TCTCCTCAAG GCCAGGCCTC GGCATTCACT GAACAGCCTC
    GCCGCCGTGG TGAAGGAGAT TGAGGAGATC CCCGCACACC TGCCCAACGG CGCGGCGCTG
    AAAGACTCTG TGCAGAGAGC CAGAGACTGG CTTCAGGAAG TGGAGGCCCT GCAGGCTGGA
    GGACGTGTGC CAGTGTTGGA CACGCTGGTG GAGCTTGTCA CTCGAGGCCG GTGTATCCCG
    GTGCATCTAC CCTCGCTGCC GAGACTGGAG TCCCTAGTAG CTGAAGTTCA CGTTTGGAAA
    GAATGTGCTG CTAATACATT CCTGACTAAG AACTCGTCCT ATTCTCTCCT AGAGGTGCTG
    TGTCCTCGAT GTGATATTGG CCTTTTGGGA TTAAAGAGGA AGCAGAGAAA GTTAAAGGAT
    CCCTTGCCAA GTGGCAAGAA GAAGAGTCCC CGTGTAGAGA GTCTGAGCGA CCTGGAGAGA
    ACGCTGATGG AAAGCAGAGA GACTGCTTCA GCTATGGCAG CACTCGGGGA AGCTCGCGCA
    CGAGAAATGG AAGCCTTGCA GTCTCTCCGA CAAGCCAATG AGGAGAAACT GCTGTCGCCT
    GTGCACGATG TGGAGATGAG AGTCTGCGTG TGTCAGAAGG CTCCCGCGGC CCCCATGGTC
    CAGTGTGAAC TCTGCAGGGA TGCTTTCCAC ACCAGCTGTG TGGCAGCTCC CAGCATTCCA
    CAGGGCCCCC GAGTCTGGCT GTGTCCCAAC TGTCGGAGGT CGGAGAAGCC TCCACTCGAG
    AAGATTCTGC CCCTGCTGGC CTCCCTGCAG CGCATCCGCG TCCGCCTGCC CGAGGGAGAC
    GCACTGCGGT ATATGATCGA GAGGACCGTG AGCTGGCAGC ACAGAGCCCG GCAGCTGCTC
    TCGTCAGGGC ACCTCAAGTC CTTGCAGGAC CCAGTGGGCT CAGGACTGTT GTGTGGCAGG
    TGGCAGGCCA CAGCAGGACA GGTGCCAGAG ACCAGCAAGA TATCTCAGCC TCCTGGCCCC
    ACGTCCTTCT CCTTGCCCGA CGACTGGGAC AACAGAACCT CCTATTTACA CTCTCCTTTC
    TCCACTGGTC GGAGCTGTAT CCCCCTCCAT GGTATCAGCC CAGAAGTGAA TGAGCTGCTG
    ATGGAGGCCC AGCTGCTCCA GGTGTCCCTT CCTGAAATTC AGGAACTCTA CCAGACGTTA
    CTTGCAAAGC CGAGCCCTGC GCAGCAGGCT GACCACAGCT CTCCAGTGAG ACCCGGCAGT
    GAGAAGAGTG ACGGCTGCCG AGGGAAGCGA GATGGAGCCA GCAGCCTCGA GAGCAAGCTG
    AAGAGACGCC TGGAAAGAGA AGGCCTCTCC AGTGAGCGGT GGGATCGAAT CACCAAAATG
    CGGGCCCCCA AAAAGAAGAG AATCAAACTG AGCCACCCCA AGGACACGGA CAGTCTCAAG
    TTAGAGAGAG AGTGTAGCTA TGAGTTGGCC CCCTCTGCCG ACACGCAGTC CCTGCCCTCA
    GACTCGTCCT CCTCGGAGCA GGAGGACTCT GAGGACGAGG ACGCCATCTG CCCGGCCGTG
    AGCTGCCTGC AGCCAGAAGG AGATGAGGTG GACTGGGTCC AGTGTGATGG CAGCTGCAGT
    CGGTGGTTCC ATCAGGTCTG TGTTGGTGTC TCTCCCGAGA CGGCCGAGGA GGAGGACTAC
    GTGTGCGTGC GCTGTGCGGA GACGGACGTG CCGGGCCGGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832344.1
    CDS183..4667
    Translation

    Target ORF information:

    RefSeq Version XM_024976576.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 5B (KDM5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGCCC CCGGCCCGCG CCCCGTGCCG GCCCTCGGCG GTCCCGGCCC ACTGGGGGAG 
    TTCCTGCCGC CGCCCGAGTG CCCGGTCTTC GAGCCCAGCT GGGAGGAGTT CGCCGATCCC
    TTCGCCTTCA TCCACAAGAT ACGGCCCATA GCCGAGCAGA CCGGGATCTG CAAGGTGCGG
    CCGCCGCCGG ATTGGCAGCC ACCATTTGCC TGTGATGTTG ACAAACTTCA TTTTACCCCG
    CGTATCCAGA GGCTGAATGA ACTGGAGGCT CAAACACGTG TAAAATTAAA TTTCCTGGAC
    CAGATTGCAA AGTACTGGGA ATTACAGGGA AGTACTCTGA AAATTCCACA CGTGGAGAGG
    AAGATCTTGG ACTTGTTTCA GCTTAATAAG TTAGTTGCAG AGGAAGGTGG CTTTGCAGTT
    GTTTGCAAGG ATAGAAAATG GACCAAAATT GCCACCAAGA TGGGCTTTGC TCCTGGCAAA
    GCTGTGGGCT CTCATATAAG AGGACACTAC GAGCGGATTC TGAACCCCTA CAACTTATTT
    CTGTCTGGAG ACAGCTTGAG GGCCATGAAT ATTAAAATAG AACCTGAAGA GACAACAGAA
    GCCAGAACTC ATAACCTGAG ACGTCGAATG GGATGTCCAG CTCCAAAGTG TGAGAATGAC
    AAAGACGTGA AAAGCAGCGT CAAGCAAGAA CCTGTTGAAA AGAAAGAGTG TGTTACAGAA
    AGTGAGAAGG AAAAACCCAA GAGTCGAGCT AAAAAAACTA CCAATGCTGT TGACCTGTAC
    GTCTGCCTCT TGTGTGGCAG TGGCAGCGAC GAGGACCGGC TTCTCCTGTG CGATGGGTGT
    GATGACAGCT ACCACACCTT CTGCCTGATC CCGCCGCTCC ATGACGTTCC CAAGGGAGAC
    TGGAGGTGTC CTAAGTGTTT GGCTCAGGAA TGCAGTAAGC CACAAGAAGC ATTCGGCTTT
    GAGCAGGCGG CCAGGGATTA TACTCTCCGT ACTTTTGGTG AAATGGCAGA TGCATTCAAA
    TCTGATTACT TCAACATGCC AGTCCATATG GTTCCCACGG AACTGGTAGA GAAGGAATTT
    TGGCGACTAG TGAGCACTAT TGAAGAGGAT GTCACCGTGG AATATGGAGC CGATATTGCC
    TCGAAGGAGT TCGGCAGTGG CTTCCCTGTC CGAGATGGGA AAGTCAAGCT CTCACCTGAG
    GAGGAGGAGT ATCTTGATAG TGGCTGGAAT CTGAACAACA TGCCAGTGAT GGAGCAGTCT
    GTTCTTGCTC ACATCACTGC CGACATATGC GGCATGAAGC TTCCTTGGCT GTACGTGGGA
    ATGTGCTTTT CGTCCTTCTG TTGGCACATT GAAGACCACT GGAGCTATTC AATTAACTAC
    CTGCACTGGG GTGAGCCAAA GACCTGGTAC GGCGTTCCCG GGTACGCTGC TGAGCAGCTG
    GAGACCGTGA TGAAGAAGCT GGCCCCAGAG CTCTTCATCT CCCAGCCCGA CCTTCTCCAC
    CAGCTTGTCA CCATCATGAA CCCCAACACC CTGATGACTC ACGAAGTGCC TGTTTACCGA
    ACCAATCAGT GTGCTGGGGA GTTTGTGATC ACGTTTCCAA GAGCCTACCA CAGCGGTTTC
    AATCAAGGTT TCAATTTTGC TGAGGCCGTT AACTTCTGCA CTGTTGATTG GCTGCCCTTA
    GGCCGGCAGT GCGTGGAGCA TTACCGTCTG CTCCACCGCT ACTGCGTGTT CTCCCACGAC
    GAGATGATCT GCAAGATGGC TTCCAAGGCG GATGTCCTAG ACGTTGTAGT GGCCTCAACT
    GTTCAGAAAG ACATGGCCAT CATGATTGAG GACGAGAAGG CACTCAGGGA AACTGTTCGC
    AAATTGGGAG TAATTGATTC GGAAAGAATG GATTTTGAAC TGTTGCCGGA TGATGAGCGT
    CAGTGTGTGA AATGCAAAAC CACGTGCTTC ATGTCTGCCC TCTCCTGCTG CTGCAAACCT
    GGCCTGCTTG TTTGCCTGCA TCACGTACAG GAGTTATGTT CCTGTCCTCC TTATAAATAC
    AAACTGAGGT ACAGATACAC TCTGGATGAC CTCTACCCCA TGATGAATGC ACTGAAGCTT
    CGAGCAGAGT CTTATAACGA GTGGGCCTTG AATGTGAATG AAGCTTTGGA GGCTAAGATT
    AATAAGAAGA AAAGCCTTGT CAGCTTTAAG GCTTTGATTG AAGAATCTGA GATGAAGAAA
    TTTCCAGACA ACGATCTTCT ACGTCACCTC CGCTTAGTCA CACAGGACGC GGAGAAATGC
    GCCTCTGTTG CCCAGCAGCT ACTTAATGGA AAGAGGCAGA CGAGATACCG GTCTGGTGGA
    GGGAAATCCC ACAATCAGCT GACCGTGAAT GAGCTCCGGC AGTTTGTGAC CCAGCTGCAC
    GCCCTCCCCT GTGTCCTCAG TCAGACACCG CTGCTGAAGG ATCTCTTGAA TCGTGTAGAA
    GATTTCCAAC AGCATAGCCA GAAACTGCTC TCTGAGGAGA TGCCCAGTGC TTCTGAGCTG
    CAGGACTTGC TGGATGTCAG CTTTGAATTT GATGTTGAGC TCCCCCAGCT TGCTGAGATG
    CGTGTCCGGC TGGAGCAGGC CCATTGGCTG GAGGAGGTGC AGCAGGCTTG CCTGGACCCG
    AGCTCCCTCA CCTTAGATGA TATGAGACGT CTCATAGACC TGGGAGTGGG CCTGGCCCCA
    TATTCAGCCG TGGAGAAGGC TATGGCGCGG CTTCAGGAAC TGCTCACAGT ATCAGAGCAC
    TGGGATGACA AAGCCAAGAG TCTCCTCAAG GCCAGGCCTC GGCATTCACT GAACAGCCTC
    GCCGCCGTGG TGAAGGAGAT TGAGGAGATC CCCGCACACC TGCCCAACGG CGCGGCGCTG
    AAAGACTCTG TGCAGAGAGC CAGAGACTGG CTTCAGGAAG TGGAGGCCCT GCAGGCTGGA
    GGACGTGTGC CAGTGTTGGA CACGCTGGTG GAGCTTGTCA CTCGAGGCCG GTGTATCCCG
    GTGCATCTAC CCTCGCTGCC GAGACTGGAG TCCCTAGTAG CTGAAGTTCA CGTTTGGAAA
    GAATGTGCTG CTAATACATT CCTGACTAAG AACTCGTCCT ATTCTCTCCT AGAGGTGCTG
    TGTCCTCGAT GTGATATTGG CCTTTTGGGA TTAAAGAGGA AGCAGAGAAA GTTAAAGGAT
    CCCTTGCCAA GTGGCAAGAA GAAGAGTCCC CGTGTAGAGA GTCTGAGCGA CCTGGAGAGA
    ACGCTGATGG AAAGCAGAGA GACTGCTTCA GCTATGGCAG CACTCGGGGA AGCTCGCGCA
    CGAGAAATGG AAGCCTTGCA GTCTCTCCGA CAAGCCAATG AGGAGAAACT GCTGTCGCCT
    GTGCACGATG TGGAGATGAG AGTCTGCGTG TGTCAGAAGG CTCCCGCGGC CCCCATGGTC
    CAGTGTGAAC TCTGCAGGGA TGCTTTCCAC ACCAGCTGTG TGGCAGCTCC CAGCATTCCA
    CAGGGCCCCC GAGTCTGGCT GTGTCCCAAC TGTCGGAGGT CGGAGAAGCC TCCACTCGAG
    AAGATTCTGC CCCTGCTGGC CTCCCTGCAG CGCATCCGCG TCCGCCTGCC CGAGGGAGAC
    GCACTGCGGT ATATGATCGA GAGGACCGTG AGCTGGCAGC ACAGAGCCCG GCAGCTGCTC
    TCGTCAGGGC ACCTCAAGTC CTTGCAGGAC CCAGTGGGCT CAGGACTGTT GTGTGGCAGG
    TGGCAGGCCA CAGCAGGACA GGTGCCAGAG ACCAGCAAGA TATCTCAGCC TCCTGGCCCC
    ACGTCCTTCT CCTTGCCCGA CGACTGGGAC AACAGAACCT CCTATTTACA CTCTCCTTTC
    TCCACTGGTC GGAGCTGTAT CCCCCTCCAT GGTATCAGCC CAGAAGTGAA TGAGCTGCTG
    ATGGAGGCCC AGCTGCTCCA GGTGTCCCTT CCTGAAATTC AGGAACTCTA CCAGACGTTA
    CTTGCAAAGC CGAGCCCTGC GCAGCAGGCT GACCACAGCT CTCCAGTGAG ACCCGGCAGT
    GAGAAGAGTG ACGGCTGCCG AGGGAAGCGA GATGGAGCCA GCAGCCTCGA GAGCAAGCTG
    AAGAGACGCC TGGAAAGAGA AGGCCTCTCC AGTGAGCGGT GGGATCGAAT CACCAAAATG
    CGGGCCCCCA AAAAGAAGAG AATCAAACTG AGCCACCCCA AGGACACGGA CAGTCTCAAG
    TTAGAGAGAG AGTGTAGCTA TGAGTTGGCC CCCTCTGCCG ACACGCAGTC CCTGCCCTCA
    GACTCGTCCT CCTCGGAGCA GGAGGACTCT GAGGACGAGG ACGCCATCTG CCCGGCCGTG
    AGCTGCCTGC AGCCAGAAGG AGATGAGGTG GACTGGGTCC AGTGTGATGG CAGCTGCAGT
    CGGTGGTTCC ATCAGGTCTG TGTTGGTGTC TCTCCCGAGA CGGCCGAGGA GGAGGACTAC
    GTGTGCGTGC GCTGTGCGGA GACGGACGTG CCGGGCCGGA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223796
    Clone ID Related Accession (Same CDS sequence) XM_024976578.1
    Accession Version XM_024976578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 5B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGCCC CCGGCCCGCG CCCCGTGCCG GCCCTCGGCG GTCCCGGCCC ACTGGGGGAG 
    TTCCTGCCGC CGCCCGAGTG CCCGGTCTTC GAGCCCAGCT GGGAGGAGTT CGCCGATCCC
    TTCGCCTTCA TCCACAAGAT ACGGCCCATA GCCGAGCAGA CCGGGATCTG CAAGGTGCGG
    CCGCCGCCGG ATTGGCAGCC ACCATTTGCC TGTGATGTTG ACAAACTTCA TTTTACCCCG
    CGTATCCAGA GGCTGAATGA ACTGGAGGCT CAAACACGTG TAAAATTAAA TTTCCTGGAC
    CAGATTGCAA AGTACTGGGA ATTACAGGGA AGTACTCTGA AAATTCCACA CGTGGAGAGG
    AAGATCTTGG ACTTGTTTCA GCTTAATAAG TTAGTTGCAG AGGAAGGTGG CTTTGCAGTT
    GTTTGCAAGG ATAGAAAATG GACCAAAATT GCCACCAAGA TGGGCTTTGC TCCTGGCAAA
    GCTGTGGGCT CTCATATAAG AGGACACTAC GAGCGGATTC TGAACCCCTA CAACTTATTT
    CTGTCTGGAG ACAGCTTGAG GGTTGACCTG TACGTCTGCC TCTTGTGTGG CAGTGGCAGC
    GACGAGGACC GGCTTCTCCT GTGCGATGGG TGTGATGACA GCTACCACAC CTTCTGCCTG
    ATCCCGCCGC TCCATGACGT TCCCAAGGGA GACTGGAGGT GTCCTAAGTG TTTGGCTCAG
    GAATGCAGTA AGCCACAAGA AGCATTCGGC TTTGAGCAGG CGGCCAGGGA TTATACTCTC
    CGTACTTTTG GTGAAATGGC AGATGCATTC AAATCTGATT ACTTCAACAT GCCAGTCCAT
    ATGGTTCCCA CGGAACTGGT AGAGAAGGAA TTTTGGCGAC TAGTGAGCAC TATTGAAGAG
    GATGTCACCG TGGAATATGG AGCCGATATT GCCTCGAAGG AGTTCGGCAG TGGCTTCCCT
    GTCCGAGATG GGAAAGTCAA GCTCTCACCT GAGGAGGAGG AGTATCTTGA TAGTGGCTGG
    AATCTGAACA ACATGCCAGT GATGGAGCAG TCTGTTCTTG CTCACATCAC TGCCGACATA
    TGCGGCATGA AGCTTCCTTG GCTGTACGTG GGAATGTGCT TTTCGTCCTT CTGTTGGCAC
    ATTGAAGACC ACTGGAGCTA TTCAATTAAC TACCTGCACT GGGGTGAGCC AAAGACCTGG
    TACGGCGTTC CCGGGTACGC TGCTGAGCAG CTGGAGACCG TGATGAAGAA GCTGGCCCCA
    GAGCTCTTCA TCTCCCAGCC CGACCTTCTC CACCAGCTTG TCACCATCAT GAACCCCAAC
    ACCCTGATGA CTCACGAAGT GCCTGTTTAC CGAACCAATC AGTGTGCTGG GGAGTTTGTG
    ATCACGTTTC CAAGAGCCTA CCACAGCGGT TTCAATCAAG GTTTCAATTT TGCTGAGGCC
    GTTAACTTCT GCACTGTTGA TTGGCTGCCC TTAGGCCGGC AGTGCGTGGA GCATTACCGT
    CTGCTCCACC GCTACTGCGT GTTCTCCCAC GACGAGATGA TCTGCAAGAT GGCTTCCAAG
    GCGGATGTCC TAGACGTTGT AGTGGCCTCA ACTGTTCAGA AAGACATGGC CATCATGATT
    GAGGACGAGA AGGCACTCAG GGAAACTGTT CGCAAATTGG GAGTAATTGA TTCGGAAAGA
    ATGGATTTTG AACTGTTGCC GGATGATGAG CGTCAGTGTG TGAAATGCAA AACCACGTGC
    TTCATGTCTG CCCTCTCCTG CTGCTGCAAA CCTGGCCTGC TTGTTTGCCT GCATCACGTA
    CAGGAGTTAT GTTCCTGTCC TCCTTATAAA TACAAACTGA GGTACAGATA CACTCTGGAT
    GACCTCTACC CCATGATGAA TGCACTGAAG CTTCGAGCAG AGTCTTATAA CGAGTGGGCC
    TTGAATGTGA ATGAAGCTTT GGAGGCTAAG ATTAATAAGA AGAAAAGCCT TGTCAGCTTT
    AAGGCTTTGA TTGAAGAATC TGAGATGAAG AAATTTCCAG ACAACGATCT TCTACGTCAC
    CTCCGCTTAG TCACACAGGA CGCGGAGAAA TGCGCCTCTG TTGCCCAGCA GCTACTTAAT
    GGAAAGAGGC AGACGAGATA CCGGTCTGGT GGAGGGAAAT CCCACAATCA GCTGACCGTG
    AATGAGCTCC GGCAGTTTGT GACCCAGCTG CACGCCCTCC CCTGTGTCCT CAGTCAGACA
    CCGCTGCTGA AGGATCTCTT GAATCGTGTA GAAGATTTCC AACAGCATAG CCAGAAACTG
    CTCTCTGAGG AGATGCCCAG TGCTTCTGAG CTGCAGGACT TGCTGGATGT CAGCTTTGAA
    TTTGATGTTG AGCTCCCCCA GCTTGCTGAG ATGCGTGTCC GGCTGGAGCA GGCCCATTGG
    CTGGAGGAGG TGCAGCAGGC TTGCCTGGAC CCGAGCTCCC TCACCTTAGA TGATATGAGA
    CGTCTCATAG ACCTGGGAGT GGGCCTGGCC CCATATTCAG CCGTGGAGAA GGCTATGGCG
    CGGCTTCAGG AACTGCTCAC AGTATCAGAG CACTGGGATG ACAAAGCCAA GAGTCTCCTC
    AAGGCCAGGC CTCGGCATTC ACTGAACAGC CTCGCCGCCG TGGTGAAGGA GATTGAGGAG
    ATCCCCGCAC ACCTGCCCAA CGGCGCGGCG CTGAAAGACT CTGTGCAGAG AGCCAGAGAC
    TGGCTTCAGG AAGTGGAGGC CCTGCAGGCT GGAGGACGTG TGCCAGTGTT GGACACGCTG
    GTGGAGCTTG TCACTCGAGG CCGGTGTATC CCGGTGCATC TACCCTCGCT GCCGAGACTG
    GAGTCCCTAG TAGCTGAAGT TCACGTTTGG AAAGAATGTG CTGCTAATAC ATTCCTGACT
    AAGAACTCGT CCTATTCTCT CCTAGAGGTG CTGTGTCCTC GATGTGATAT TGGCCTTTTG
    GGATTAAAGA GGAAGCAGAG AAAGTTAAAG GATCCCTTGC CAAGTGGCAA GAAGAAGAGT
    CCCCGTGTAG AGAGTCTGAG CGACCTGGAG AGAACGCTGA TGGAAAGCAG AGAGACTGCT
    TCAGCTATGG CAGCACTCGG GGAAGCTCGC GCACGAGAAA TGGAAGCCTT GCAGTCTCTC
    CGACAAGCCA ATGAGGAGAA ACTGCTGTCG CCTGTGCACG ATGTGGAGAT GAGAGTCTGC
    GTGTGTCAGA AGGCTCCCGC GGCCCCCATG GTCCAGTGTG AACTCTGCAG GGATGCTTTC
    CACACCAGCT GTGTGGCAGC TCCCAGCATT CCACAGGGCC CCCGAGTCTG GCTGTGTCCC
    AACTGTCGGA GGTCGGAGAA GCCTCCACTC GAGAAGATTC TGCCCCTGCT GGCCTCCCTG
    CAGCGCATCC GCGTCCGCCT GCCCGAGGGA GACGCACTGC GGTATATGAT CGAGAGGACC
    GTGAGCTGGC AGCACAGAGC CCGGCAGCTG CTCTCGTCAG GGCACCTCAA GTCCTTGCAG
    GACCCAGTGG GCTCAGGACT GTTGTGTGGC AGGTGGCAGG CCACAGCAGG ACAGGTGCCA
    GAGACCAGCA AGATATCTCA GCCTCCTGGC CCCACGTCCT TCTCCTTGCC CGACGACTGG
    GACAACAGAA CCTCCTATTT ACACTCTCCT TTCTCCACTG GTCGGAGCTG TATCCCCCTC
    CATGGTATCA GCCCAGAAGT GAATGAGCTG CTGATGGAGG CCCAGCTGCT CCAGGTGTCC
    CTTCCTGAAA TTCAGGAACT CTACCAGACG TTACTTGCAA AGCCGAGCCC TGCGCAGCAG
    GCTGACCACA GCTCTCCAGT GAGACCCGGC AGTGAGAAGA GTGACGGCTG CCGAGGGAAG
    CGAGATGGAG CCAGCAGCCT CGAGAGCAAG CTGAAGAGAC GCCTGGAAAG AGAAGGCCTC
    TCCAGTGAGC GGTGGGATCG AATCACCAAA ATGCGGGCCC CCAAAAAGAA GAGAATCAAA
    CTGAGCCACC CCAAGGACAC GGACAGTCTC AAGTTAGAGA GAGAGTGTAG CTATGAGTTG
    GCCCCCTCTG CCGACACGCA GTCCCTGCCC TCAGACTCGT CCTCCTCGGA GCAGGAGGAC
    TCTGAGGACG AGGACGCCAT CTGCCCGGCC GTGAGCTGCC TGCAGCCAGA AGGAGATGAG
    GTGGACTGGG TCCAGTGTGA TGGCAGCTGC AGTCGGTGGT TCCATCAGGT CTGTGTTGGT
    GTCTCTCCCG AGACGGCCGA GGAGGAGGAC TACGTGTGCG TGCGCTGTGC GGAGACGGAC
    GTGCCGGGCC GGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832346.1
    CDS183..4460
    Translation

    Target ORF information:

    RefSeq Version XM_024976578.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 5B (KDM5B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGGCCC CCGGCCCGCG CCCCGTGCCG GCCCTCGGCG GTCCCGGCCC ACTGGGGGAG 
    TTCCTGCCGC CGCCCGAGTG CCCGGTCTTC GAGCCCAGCT GGGAGGAGTT CGCCGATCCC
    TTCGCCTTCA TCCACAAGAT ACGGCCCATA GCCGAGCAGA CCGGGATCTG CAAGGTGCGG
    CCGCCGCCGG ATTGGCAGCC ACCATTTGCC TGTGATGTTG ACAAACTTCA TTTTACCCCG
    CGTATCCAGA GGCTGAATGA ACTGGAGGCT CAAACACGTG TAAAATTAAA TTTCCTGGAC
    CAGATTGCAA AGTACTGGGA ATTACAGGGA AGTACTCTGA AAATTCCACA CGTGGAGAGG
    AAGATCTTGG ACTTGTTTCA GCTTAATAAG TTAGTTGCAG AGGAAGGTGG CTTTGCAGTT
    GTTTGCAAGG ATAGAAAATG GACCAAAATT GCCACCAAGA TGGGCTTTGC TCCTGGCAAA
    GCTGTGGGCT CTCATATAAG AGGACACTAC GAGCGGATTC TGAACCCCTA CAACTTATTT
    CTGTCTGGAG ACAGCTTGAG GGTTGACCTG TACGTCTGCC TCTTGTGTGG CAGTGGCAGC
    GACGAGGACC GGCTTCTCCT GTGCGATGGG TGTGATGACA GCTACCACAC CTTCTGCCTG
    ATCCCGCCGC TCCATGACGT TCCCAAGGGA GACTGGAGGT GTCCTAAGTG TTTGGCTCAG
    GAATGCAGTA AGCCACAAGA AGCATTCGGC TTTGAGCAGG CGGCCAGGGA TTATACTCTC
    CGTACTTTTG GTGAAATGGC AGATGCATTC AAATCTGATT ACTTCAACAT GCCAGTCCAT
    ATGGTTCCCA CGGAACTGGT AGAGAAGGAA TTTTGGCGAC TAGTGAGCAC TATTGAAGAG
    GATGTCACCG TGGAATATGG AGCCGATATT GCCTCGAAGG AGTTCGGCAG TGGCTTCCCT
    GTCCGAGATG GGAAAGTCAA GCTCTCACCT GAGGAGGAGG AGTATCTTGA TAGTGGCTGG
    AATCTGAACA ACATGCCAGT GATGGAGCAG TCTGTTCTTG CTCACATCAC TGCCGACATA
    TGCGGCATGA AGCTTCCTTG GCTGTACGTG GGAATGTGCT TTTCGTCCTT CTGTTGGCAC
    ATTGAAGACC ACTGGAGCTA TTCAATTAAC TACCTGCACT GGGGTGAGCC AAAGACCTGG
    TACGGCGTTC CCGGGTACGC TGCTGAGCAG CTGGAGACCG TGATGAAGAA GCTGGCCCCA
    GAGCTCTTCA TCTCCCAGCC CGACCTTCTC CACCAGCTTG TCACCATCAT GAACCCCAAC
    ACCCTGATGA CTCACGAAGT GCCTGTTTAC CGAACCAATC AGTGTGCTGG GGAGTTTGTG
    ATCACGTTTC CAAGAGCCTA CCACAGCGGT TTCAATCAAG GTTTCAATTT TGCTGAGGCC
    GTTAACTTCT GCACTGTTGA TTGGCTGCCC TTAGGCCGGC AGTGCGTGGA GCATTACCGT
    CTGCTCCACC GCTACTGCGT GTTCTCCCAC GACGAGATGA TCTGCAAGAT GGCTTCCAAG
    GCGGATGTCC TAGACGTTGT AGTGGCCTCA ACTGTTCAGA AAGACATGGC CATCATGATT
    GAGGACGAGA AGGCACTCAG GGAAACTGTT CGCAAATTGG GAGTAATTGA TTCGGAAAGA
    ATGGATTTTG AACTGTTGCC GGATGATGAG CGTCAGTGTG TGAAATGCAA AACCACGTGC
    TTCATGTCTG CCCTCTCCTG CTGCTGCAAA CCTGGCCTGC TTGTTTGCCT GCATCACGTA
    CAGGAGTTAT GTTCCTGTCC TCCTTATAAA TACAAACTGA GGTACAGATA CACTCTGGAT
    GACCTCTACC CCATGATGAA TGCACTGAAG CTTCGAGCAG AGTCTTATAA CGAGTGGGCC
    TTGAATGTGA ATGAAGCTTT GGAGGCTAAG ATTAATAAGA AGAAAAGCCT TGTCAGCTTT
    AAGGCTTTGA TTGAAGAATC TGAGATGAAG AAATTTCCAG ACAACGATCT TCTACGTCAC
    CTCCGCTTAG TCACACAGGA CGCGGAGAAA TGCGCCTCTG TTGCCCAGCA GCTACTTAAT
    GGAAAGAGGC AGACGAGATA CCGGTCTGGT GGAGGGAAAT CCCACAATCA GCTGACCGTG
    AATGAGCTCC GGCAGTTTGT GACCCAGCTG CACGCCCTCC CCTGTGTCCT CAGTCAGACA
    CCGCTGCTGA AGGATCTCTT GAATCGTGTA GAAGATTTCC AACAGCATAG CCAGAAACTG
    CTCTCTGAGG AGATGCCCAG TGCTTCTGAG CTGCAGGACT TGCTGGATGT CAGCTTTGAA
    TTTGATGTTG AGCTCCCCCA GCTTGCTGAG ATGCGTGTCC GGCTGGAGCA GGCCCATTGG
    CTGGAGGAGG TGCAGCAGGC TTGCCTGGAC CCGAGCTCCC TCACCTTAGA TGATATGAGA
    CGTCTCATAG ACCTGGGAGT GGGCCTGGCC CCATATTCAG CCGTGGAGAA GGCTATGGCG
    CGGCTTCAGG AACTGCTCAC AGTATCAGAG CACTGGGATG ACAAAGCCAA GAGTCTCCTC
    AAGGCCAGGC CTCGGCATTC ACTGAACAGC CTCGCCGCCG TGGTGAAGGA GATTGAGGAG
    ATCCCCGCAC ACCTGCCCAA CGGCGCGGCG CTGAAAGACT CTGTGCAGAG AGCCAGAGAC
    TGGCTTCAGG AAGTGGAGGC CCTGCAGGCT GGAGGACGTG TGCCAGTGTT GGACACGCTG
    GTGGAGCTTG TCACTCGAGG CCGGTGTATC CCGGTGCATC TACCCTCGCT GCCGAGACTG
    GAGTCCCTAG TAGCTGAAGT TCACGTTTGG AAAGAATGTG CTGCTAATAC ATTCCTGACT
    AAGAACTCGT CCTATTCTCT CCTAGAGGTG CTGTGTCCTC GATGTGATAT TGGCCTTTTG
    GGATTAAAGA GGAAGCAGAG AAAGTTAAAG GATCCCTTGC CAAGTGGCAA GAAGAAGAGT
    CCCCGTGTAG AGAGTCTGAG CGACCTGGAG AGAACGCTGA TGGAAAGCAG AGAGACTGCT
    TCAGCTATGG CAGCACTCGG GGAAGCTCGC GCACGAGAAA TGGAAGCCTT GCAGTCTCTC
    CGACAAGCCA ATGAGGAGAA ACTGCTGTCG CCTGTGCACG ATGTGGAGAT GAGAGTCTGC
    GTGTGTCAGA AGGCTCCCGC GGCCCCCATG GTCCAGTGTG AACTCTGCAG GGATGCTTTC
    CACACCAGCT GTGTGGCAGC TCCCAGCATT CCACAGGGCC CCCGAGTCTG GCTGTGTCCC
    AACTGTCGGA GGTCGGAGAA GCCTCCACTC GAGAAGATTC TGCCCCTGCT GGCCTCCCTG
    CAGCGCATCC GCGTCCGCCT GCCCGAGGGA GACGCACTGC GGTATATGAT CGAGAGGACC
    GTGAGCTGGC AGCACAGAGC CCGGCAGCTG CTCTCGTCAG GGCACCTCAA GTCCTTGCAG
    GACCCAGTGG GCTCAGGACT GTTGTGTGGC AGGTGGCAGG CCACAGCAGG ACAGGTGCCA
    GAGACCAGCA AGATATCTCA GCCTCCTGGC CCCACGTCCT TCTCCTTGCC CGACGACTGG
    GACAACAGAA CCTCCTATTT ACACTCTCCT TTCTCCACTG GTCGGAGCTG TATCCCCCTC
    CATGGTATCA GCCCAGAAGT GAATGAGCTG CTGATGGAGG CCCAGCTGCT CCAGGTGTCC
    CTTCCTGAAA TTCAGGAACT CTACCAGACG TTACTTGCAA AGCCGAGCCC TGCGCAGCAG
    GCTGACCACA GCTCTCCAGT GAGACCCGGC AGTGAGAAGA GTGACGGCTG CCGAGGGAAG
    CGAGATGGAG CCAGCAGCCT CGAGAGCAAG CTGAAGAGAC GCCTGGAAAG AGAAGGCCTC
    TCCAGTGAGC GGTGGGATCG AATCACCAAA ATGCGGGCCC CCAAAAAGAA GAGAATCAAA
    CTGAGCCACC CCAAGGACAC GGACAGTCTC AAGTTAGAGA GAGAGTGTAG CTATGAGTTG
    GCCCCCTCTG CCGACACGCA GTCCCTGCCC TCAGACTCGT CCTCCTCGGA GCAGGAGGAC
    TCTGAGGACG AGGACGCCAT CTGCCCGGCC GTGAGCTGCC TGCAGCCAGA AGGAGATGAG
    GTGGACTGGG TCCAGTGTGA TGGCAGCTGC AGTCGGTGGT TCCATCAGGT CTGTGTTGGT
    GTCTCTCCCG AGACGGCCGA GGAGGAGGAC TACGTGTGCG TGCGCTGTGC GGAGACGGAC
    GTGCCGGGCC GGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93014
    Clone ID Related Accession (Same CDS sequence) XM_015466901.1 , XM_015475206.1
    Accession Version XM_015466901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGTGTCTGCA GAAGCCAAAC
    CTGAGCACAG ACACAAAGGA CAAGGAGTAC AAACCCCATG GTATTCCCCA GAGGCAGTCT
    GTGCAGCCCG CGGAAACCTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCGGAGGCC
    ATGAATATTA AAATAGAACC TGAAGAGACA ACAGAAGCCA GAACTCATAA CCTGAGACGT
    CGAATGGGAT GTCCAGCTCC AAAGTGTGAG AATGACAAAG ACGTGAAAAG CAGCGTCAAG
    CAAGAACCTG TTGAAAAGAA AGAGTGTGTT ACAGAAAGTG AGAAGGAAAA ACCCAAGAGT
    CGAGCTAAAA AAACTACCAA TGCTGTTGAC CTGTACGTCT GCCTCTTGTG TGGCAGTGGC
    AGCGACGAGG ACCGGCTTCT CCTGTGCGAT GGGTGTGATG ACAGCTACCA CACCTTCTGC
    CTGATCCCGC CGCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT
    CAGGAATGCA GTAAGCCACA AGAAGCATTC GGCTTTGAGC AGGCGGCCAG GGATTATACT
    CTCCGTACTT TTGGTGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC
    CATATGGTTC CCACGGAACT GGTAGAGAAG GAATTTTGGC GACTAGTGAG CACTATTGAA
    GAGGATGTCA CCGTGGAATA TGGAGCCGAT ATTGCCTCGA AGGAGTTCGG CAGTGGCTTC
    CCTGTCCGAG ATGGGAAAGT CAAGCTCTCA CCTGAGGAGG AGGAGTATCT TGATAGTGGC
    TGGAATCTGA ACAACATGCC AGTGATGGAG CAGTCTGTTC TTGCTCACAT CACTGCCGAC
    ATATGCGGCA TGAAGCTTCC TTGGCTGTAC GTGGGAATGT GCTTTTCGTC CTTCTGTTGG
    CACATTGAAG ACCACTGGAG CTATTCAATT AACTACCTGC ACTGGGGTGA GCCAAAGACC
    TGGTACGGCG TTCCCGGGTA CGCTGCTGAG CAGCTGGAGA CCGTGATGAA GAAGCTGGCC
    CCAGAGCTCT TCATCTCCCA GCCCGACCTT CTCCACCAGC TTGTCACCAT CATGAACCCC
    AACACCCTGA TGACTCACGA AGTGCCTGTT TACCGAACCA ATCAGTGTGC TGGGGAGTTT
    GTGATCACGT TTCCAAGAGC CTACCACAGC GGTTTCAATC AAGGTTTCAA TTTTGCTGAG
    GCCGTTAACT TCTGCACTGT TGATTGGCTG CCCTTAGGCC GGCAGTGCGT GGAGCATTAC
    CGTCTGCTCC ACCGCTACTG CGTGTTCTCC CACGACGAGA TGATCTGCAA GATGGCTTCC
    AAGGCGGATG TCCTAGACGT TGTAGTGGCC TCAACTGTTC AGAAAGACAT GGCCATCATG
    ATTGAGGACG AGAAGGCACT CAGGGAAACT GTTCGCAAAT TGGGAGTAAT TGATTCGGAA
    AGAATGGATT TTGAACTGTT GCCGGATGAT GAGCGTCAGT GTGTGAAATG CAAAACCACG
    TGCTTCATGT CTGCCCTCTC CTGCTGCTGC AAACCTGGCC TGCTTGTTTG CCTGCATCAC
    GTACAGGAGT TATGTTCCTG TCCTCCTTAT AAATACAAAC TGAGGTACAG ATACACTCTG
    GATGACCTCT ACCCCATGAT GAATGCACTG AAGCTTCGAG CAGAGTCTTA TAACGAGTGG
    GCCTTGAATG TGAATGAAGC TTTGGAGGCT AAGATTAATA AGAAGAAAAG CCTTGTCAGC
    TTTAAGGCTT TGATTGAAGA ATCTGAGATG AAGAAATTTC CAGACAACGA TCTTCTACGT
    CACCTCCGCT TAGTCACACA GGACGCGGAG AAATGCGCCT CTGTTGCCCA GCAGCTACTT
    AATGGAAAGA GGCAGACGAG ATACCGGTCT GGTGGAGGGA AATCCCACAA TCAGCTGACC
    GTGAATGAGC TCCGGCAGTT TGTGACCCAG CTGCACGCCC TCCCCTGTGT CCTCAGTCAG
    ACACCGCTGC TGAAGGATCT CTTGAATCGT GTAGAAGATT TCCAACAGCA TAGCCAGAAA
    CTGCTCTCTG AGGAGATGCC CAGTGCTTCT GAGCTGCAGG ACTTGCTGGA TGTCAGCTTT
    GAATTTGATG TTGAGCTCCC CCAGCTTGCT GAGATGCGTG TCCGGCTGGA GCAGGCCCAT
    TGGCTGGAGG AGGTGCAGCA GGCTTGCCTG GACCCGAGCT CCCTCACCTT AGATGATATG
    AGACGTCTCA TAGACCTGGG AGTGGGCCTG GCCCCATATT CAGCCGTGGA GAAGGCTATG
    GCGCGGCTTC AGGAACTGCT CACAGTATCA GAGCACTGGG ATGACAAAGC CAAGAGTCTC
    CTCAAGGCCA GGCCTCGGCA TTCACTGAAC AGCCTCGCCG CCGTGGTGAA GGAGATTGAG
    GAGATCCCCG CACACCTGCC CAACGGCGCG GCGCTGAAAG ACTCTGTGCA GAGAGCCAGA
    GACTGGCTTC AGGAAGTGGA GGCCCTGCAG GCTGGAGGAC GTGTGCCAGT GTTGGACACG
    CTGGTGGAGC TTGTCACTCG AGGCCGGTGT ATCCCGGTGC ATCTACCCTC GCTGCCGAGA
    CTGGAGTCCC TAGTAGCTGA AGTTCACGTT TGGAAAGAAT GTGCTGCTAA TACATTCCTG
    ACTAAGAACT CGTCCTATTC TCTCCTAGAG GTGCTGTGTC CTCGATGTGA TATTGGCCTT
    TTGGGATTAA AGAGGAAGCA GAGAAAGTTA AAGGATCCCT TGCCAAGTGG CAAGAAGAAG
    AGTCCCCGTG TAGAGAGTCT GAGCGACCTG GAGAGAACGC TGATGGAAAG CAGAGAGACT
    GCTTCAGCTA TGGCAGCACT CGGGGAAGCT CGCGCACGAG AAATGGAAGC CTTGCAGTCT
    CTCCGACAAG CCAATGAGGA GAAACTGCTG TCGCCTGTGC ACGATGTGGA GATGAGAGTC
    TGCGTGTGTC AGAAGGCTCC CGCGGCCCCC ATGGTCCAGT GTGAACTCTG CAGGGATGCT
    TTCCACACCA GCTGTGTGGC AGCTCCCAGC ATTCCACAGG GCCCCCGAGT CTGGCTGTGT
    CCCAACTGTC GGAGGTCGGA GAAGCCTCCA CTCGAGAAGA TTCTGCCCCT GCTGGCCTCC
    CTGCAGCGCA TCCGCGTCCG CCTGCCCGAG GGAGACGCAC TGCGGTATAT GATCGAGAGG
    ACCGTGAGCT GGCAGCACAG AGCCCGGCAG CTGCTCTCGT CAGGGCACCT CAAGTCCTTG
    CAGGACCCAG TGGGCTCAGG ACTGTTGTGT GGCAGGTGGC AGGCCACAGC AGGACAGGTG
    CCAGAGACCA GCAAGATGTC TCAGCCTCCT GGCCCCACGT CCTTCTCCTT GCCCGACGAC
    TGGGACAACA GAACCTCCTA TTTACACTCT CCTTTCTCCA CTGGTCGGAG CTGTATCCCC
    CTCCATGGTA TCAGCCCAGA AGTGAATGAG CTGCTGATGG AGGCCCAGCT GCTCCAGGTG
    TCCCTTCCTG AAATTCAGGA ACTCTACCAG ACGTTACTTG CAAAGCCGAG CCCTGCGCAG
    CAGGCTGACC ACAGCTCTCC AGTGAGACCC GGCAGTGAGA AGAGTGACGG CTGCCGAGGG
    AAGCGAGATG GAGCCAGCAG CCTCGAGAGC AAGCTGAAGA GACGCCTGGA AAGAGAAGGC
    CTCTCCAGTG AGCGGTGGGA TCGAATCACC AAAATGCGGG CCCCCAAAAA GAAGAGAATC
    AAACTGAGCC ACCCCAAGGA CACGGACAGT CTCAAGTTAG AGAGAGAGTG TAGCTATGAG
    TTGGCCCCCT CTGCCGACAC GCAGTCCCTG CCCTCAGACT CGTCCTCCTC GGAGCAGGAG
    GACTCTGAGG ACGAGGACGC CATCTGCCCG GCCGTGAGCT GCCTGCAGCC AGAAGGAGAT
    GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAGTCGGT GGTTCCATCA GGTCTGTGTT
    GGTGTCTCTC CCGAGACGGC CGAGGAGGAG GACTACGTGT GCGTGCGCTG TGCGGAGACG
    GACGTGCCGG GCCGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322387.1
    CDS620..4780
    Translation

    Target ORF information:

    RefSeq Version XM_015466901.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGTGTCTGCA GAAGCCAAAC
    CTGAGCACAG ACACAAAGGA CAAGGAGTAC AAACCCCATG GTATTCCCCA GAGGCAGTCT
    GTGCAGCCCG CGGAAACCTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCGGAGGCC
    ATGAATATTA AAATAGAACC TGAAGAGACA ACAGAAGCCA GAACTCATAA CCTGAGACGT
    CGAATGGGAT GTCCAGCTCC AAAGTGTGAG AATGACAAAG ACGTGAAAAG CAGCGTCAAG
    CAAGAACCTG TTGAAAAGAA AGAGTGTGTT ACAGAAAGTG AGAAGGAAAA ACCCAAGAGT
    CGAGCTAAAA AAACTACCAA TGCTGTTGAC CTGTACGTCT GCCTCTTGTG TGGCAGTGGC
    AGCGACGAGG ACCGGCTTCT CCTGTGCGAT GGGTGTGATG ACAGCTACCA CACCTTCTGC
    CTGATCCCGC CGCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT
    CAGGAATGCA GTAAGCCACA AGAAGCATTC GGCTTTGAGC AGGCGGCCAG GGATTATACT
    CTCCGTACTT TTGGTGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC
    CATATGGTTC CCACGGAACT GGTAGAGAAG GAATTTTGGC GACTAGTGAG CACTATTGAA
    GAGGATGTCA CCGTGGAATA TGGAGCCGAT ATTGCCTCGA AGGAGTTCGG CAGTGGCTTC
    CCTGTCCGAG ATGGGAAAGT CAAGCTCTCA CCTGAGGAGG AGGAGTATCT TGATAGTGGC
    TGGAATCTGA ACAACATGCC AGTGATGGAG CAGTCTGTTC TTGCTCACAT CACTGCCGAC
    ATATGCGGCA TGAAGCTTCC TTGGCTGTAC GTGGGAATGT GCTTTTCGTC CTTCTGTTGG
    CACATTGAAG ACCACTGGAG CTATTCAATT AACTACCTGC ACTGGGGTGA GCCAAAGACC
    TGGTACGGCG TTCCCGGGTA CGCTGCTGAG CAGCTGGAGA CCGTGATGAA GAAGCTGGCC
    CCAGAGCTCT TCATCTCCCA GCCCGACCTT CTCCACCAGC TTGTCACCAT CATGAACCCC
    AACACCCTGA TGACTCACGA AGTGCCTGTT TACCGAACCA ATCAGTGTGC TGGGGAGTTT
    GTGATCACGT TTCCAAGAGC CTACCACAGC GGTTTCAATC AAGGTTTCAA TTTTGCTGAG
    GCCGTTAACT TCTGCACTGT TGATTGGCTG CCCTTAGGCC GGCAGTGCGT GGAGCATTAC
    CGTCTGCTCC ACCGCTACTG CGTGTTCTCC CACGACGAGA TGATCTGCAA GATGGCTTCC
    AAGGCGGATG TCCTAGACGT TGTAGTGGCC TCAACTGTTC AGAAAGACAT GGCCATCATG
    ATTGAGGACG AGAAGGCACT CAGGGAAACT GTTCGCAAAT TGGGAGTAAT TGATTCGGAA
    AGAATGGATT TTGAACTGTT GCCGGATGAT GAGCGTCAGT GTGTGAAATG CAAAACCACG
    TGCTTCATGT CTGCCCTCTC CTGCTGCTGC AAACCTGGCC TGCTTGTTTG CCTGCATCAC
    GTACAGGAGT TATGTTCCTG TCCTCCTTAT AAATACAAAC TGAGGTACAG ATACACTCTG
    GATGACCTCT ACCCCATGAT GAATGCACTG AAGCTTCGAG CAGAGTCTTA TAACGAGTGG
    GCCTTGAATG TGAATGAAGC TTTGGAGGCT AAGATTAATA AGAAGAAAAG CCTTGTCAGC
    TTTAAGGCTT TGATTGAAGA ATCTGAGATG AAGAAATTTC CAGACAACGA TCTTCTACGT
    CACCTCCGCT TAGTCACACA GGACGCGGAG AAATGCGCCT CTGTTGCCCA GCAGCTACTT
    AATGGAAAGA GGCAGACGAG ATACCGGTCT GGTGGAGGGA AATCCCACAA TCAGCTGACC
    GTGAATGAGC TCCGGCAGTT TGTGACCCAG CTGCACGCCC TCCCCTGTGT CCTCAGTCAG
    ACACCGCTGC TGAAGGATCT CTTGAATCGT GTAGAAGATT TCCAACAGCA TAGCCAGAAA
    CTGCTCTCTG AGGAGATGCC CAGTGCTTCT GAGCTGCAGG ACTTGCTGGA TGTCAGCTTT
    GAATTTGATG TTGAGCTCCC CCAGCTTGCT GAGATGCGTG TCCGGCTGGA GCAGGCCCAT
    TGGCTGGAGG AGGTGCAGCA GGCTTGCCTG GACCCGAGCT CCCTCACCTT AGATGATATG
    AGACGTCTCA TAGACCTGGG AGTGGGCCTG GCCCCATATT CAGCCGTGGA GAAGGCTATG
    GCGCGGCTTC AGGAACTGCT CACAGTATCA GAGCACTGGG ATGACAAAGC CAAGAGTCTC
    CTCAAGGCCA GGCCTCGGCA TTCACTGAAC AGCCTCGCCG CCGTGGTGAA GGAGATTGAG
    GAGATCCCCG CACACCTGCC CAACGGCGCG GCGCTGAAAG ACTCTGTGCA GAGAGCCAGA
    GACTGGCTTC AGGAAGTGGA GGCCCTGCAG GCTGGAGGAC GTGTGCCAGT GTTGGACACG
    CTGGTGGAGC TTGTCACTCG AGGCCGGTGT ATCCCGGTGC ATCTACCCTC GCTGCCGAGA
    CTGGAGTCCC TAGTAGCTGA AGTTCACGTT TGGAAAGAAT GTGCTGCTAA TACATTCCTG
    ACTAAGAACT CGTCCTATTC TCTCCTAGAG GTGCTGTGTC CTCGATGTGA TATTGGCCTT
    TTGGGATTAA AGAGGAAGCA GAGAAAGTTA AAGGATCCCT TGCCAAGTGG CAAGAAGAAG
    AGTCCCCGTG TAGAGAGTCT GAGCGACCTG GAGAGAACGC TGATGGAAAG CAGAGAGACT
    GCTTCAGCTA TGGCAGCACT CGGGGAAGCT CGCGCACGAG AAATGGAAGC CTTGCAGTCT
    CTCCGACAAG CCAATGAGGA GAAACTGCTG TCGCCTGTGC ACGATGTGGA GATGAGAGTC
    TGCGTGTGTC AGAAGGCTCC CGCGGCCCCC ATGGTCCAGT GTGAACTCTG CAGGGATGCT
    TTCCACACCA GCTGTGTGGC AGCTCCCAGC ATTCCACAGG GCCCCCGAGT CTGGCTGTGT
    CCCAACTGTC GGAGGTCGGA GAAGCCTCCA CTCGAGAAGA TTCTGCCCCT GCTGGCCTCC
    CTGCAGCGCA TCCGCGTCCG CCTGCCCGAG GGAGACGCAC TGCGGTATAT GATCGAGAGG
    ACCGTGAGCT GGCAGCACAG AGCCCGGCAG CTGCTCTCGT CAGGGCACCT CAAGTCCTTG
    CAGGACCCAG TGGGCTCAGG ACTGTTGTGT GGCAGGTGGC AGGCCACAGC AGGACAGGTG
    CCAGAGACCA GCAAGATGTC TCAGCCTCCT GGCCCCACGT CCTTCTCCTT GCCCGACGAC
    TGGGACAACA GAACCTCCTA TTTACACTCT CCTTTCTCCA CTGGTCGGAG CTGTATCCCC
    CTCCATGGTA TCAGCCCAGA AGTGAATGAG CTGCTGATGG AGGCCCAGCT GCTCCAGGTG
    TCCCTTCCTG AAATTCAGGA ACTCTACCAG ACGTTACTTG CAAAGCCGAG CCCTGCGCAG
    CAGGCTGACC ACAGCTCTCC AGTGAGACCC GGCAGTGAGA AGAGTGACGG CTGCCGAGGG
    AAGCGAGATG GAGCCAGCAG CCTCGAGAGC AAGCTGAAGA GACGCCTGGA AAGAGAAGGC
    CTCTCCAGTG AGCGGTGGGA TCGAATCACC AAAATGCGGG CCCCCAAAAA GAAGAGAATC
    AAACTGAGCC ACCCCAAGGA CACGGACAGT CTCAAGTTAG AGAGAGAGTG TAGCTATGAG
    TTGGCCCCCT CTGCCGACAC GCAGTCCCTG CCCTCAGACT CGTCCTCCTC GGAGCAGGAG
    GACTCTGAGG ACGAGGACGC CATCTGCCCG GCCGTGAGCT GCCTGCAGCC AGAAGGAGAT
    GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAGTCGGT GGTTCCATCA GGTCTGTGTT
    GGTGTCTCTC CCGAGACGGC CGAGGAGGAG GACTACGTGT GCGTGCGCTG TGCGGAGACG
    GACGTGCCGG GCCGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93014
    Clone ID Related Accession (Same CDS sequence) XM_015466901.1 , XM_015475206.1
    Accession Version XM_015475206.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGTGTCTGCA GAAGCCAAAC
    CTGAGCACAG ACACAAAGGA CAAGGAGTAC AAACCCCATG GTATTCCCCA GAGGCAGTCT
    GTGCAGCCCG CGGAAACCTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCGGAGGCC
    ATGAATATTA AAATAGAACC TGAAGAGACA ACAGAAGCCA GAACTCATAA CCTGAGACGT
    CGAATGGGAT GTCCAGCTCC AAAGTGTGAG AATGACAAAG ACGTGAAAAG CAGCGTCAAG
    CAAGAACCTG TTGAAAAGAA AGAGTGTGTT ACAGAAAGTG AGAAGGAAAA ACCCAAGAGT
    CGAGCTAAAA AAACTACCAA TGCTGTTGAC CTGTACGTCT GCCTCTTGTG TGGCAGTGGC
    AGCGACGAGG ACCGGCTTCT CCTGTGCGAT GGGTGTGATG ACAGCTACCA CACCTTCTGC
    CTGATCCCGC CGCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT
    CAGGAATGCA GTAAGCCACA AGAAGCATTC GGCTTTGAGC AGGCGGCCAG GGATTATACT
    CTCCGTACTT TTGGTGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC
    CATATGGTTC CCACGGAACT GGTAGAGAAG GAATTTTGGC GACTAGTGAG CACTATTGAA
    GAGGATGTCA CCGTGGAATA TGGAGCCGAT ATTGCCTCGA AGGAGTTCGG CAGTGGCTTC
    CCTGTCCGAG ATGGGAAAGT CAAGCTCTCA CCTGAGGAGG AGGAGTATCT TGATAGTGGC
    TGGAATCTGA ACAACATGCC AGTGATGGAG CAGTCTGTTC TTGCTCACAT CACTGCCGAC
    ATATGCGGCA TGAAGCTTCC TTGGCTGTAC GTGGGAATGT GCTTTTCGTC CTTCTGTTGG
    CACATTGAAG ACCACTGGAG CTATTCAATT AACTACCTGC ACTGGGGTGA GCCAAAGACC
    TGGTACGGCG TTCCCGGGTA CGCTGCTGAG CAGCTGGAGA CCGTGATGAA GAAGCTGGCC
    CCAGAGCTCT TCATCTCCCA GCCCGACCTT CTCCACCAGC TTGTCACCAT CATGAACCCC
    AACACCCTGA TGACTCACGA AGTGCCTGTT TACCGAACCA ATCAGTGTGC TGGGGAGTTT
    GTGATCACGT TTCCAAGAGC CTACCACAGC GGTTTCAATC AAGGTTTCAA TTTTGCTGAG
    GCCGTTAACT TCTGCACTGT TGATTGGCTG CCCTTAGGCC GGCAGTGCGT GGAGCATTAC
    CGTCTGCTCC ACCGCTACTG CGTGTTCTCC CACGACGAGA TGATCTGCAA GATGGCTTCC
    AAGGCGGATG TCCTAGACGT TGTAGTGGCC TCAACTGTTC AGAAAGACAT GGCCATCATG
    ATTGAGGACG AGAAGGCACT CAGGGAAACT GTTCGCAAAT TGGGAGTAAT TGATTCGGAA
    AGAATGGATT TTGAACTGTT GCCGGATGAT GAGCGTCAGT GTGTGAAATG CAAAACCACG
    TGCTTCATGT CTGCCCTCTC CTGCTGCTGC AAACCTGGCC TGCTTGTTTG CCTGCATCAC
    GTACAGGAGT TATGTTCCTG TCCTCCTTAT AAATACAAAC TGAGGTACAG ATACACTCTG
    GATGACCTCT ACCCCATGAT GAATGCACTG AAGCTTCGAG CAGAGTCTTA TAACGAGTGG
    GCCTTGAATG TGAATGAAGC TTTGGAGGCT AAGATTAATA AGAAGAAAAG CCTTGTCAGC
    TTTAAGGCTT TGATTGAAGA ATCTGAGATG AAGAAATTTC CAGACAACGA TCTTCTACGT
    CACCTCCGCT TAGTCACACA GGACGCGGAG AAATGCGCCT CTGTTGCCCA GCAGCTACTT
    AATGGAAAGA GGCAGACGAG ATACCGGTCT GGTGGAGGGA AATCCCACAA TCAGCTGACC
    GTGAATGAGC TCCGGCAGTT TGTGACCCAG CTGCACGCCC TCCCCTGTGT CCTCAGTCAG
    ACACCGCTGC TGAAGGATCT CTTGAATCGT GTAGAAGATT TCCAACAGCA TAGCCAGAAA
    CTGCTCTCTG AGGAGATGCC CAGTGCTTCT GAGCTGCAGG ACTTGCTGGA TGTCAGCTTT
    GAATTTGATG TTGAGCTCCC CCAGCTTGCT GAGATGCGTG TCCGGCTGGA GCAGGCCCAT
    TGGCTGGAGG AGGTGCAGCA GGCTTGCCTG GACCCGAGCT CCCTCACCTT AGATGATATG
    AGACGTCTCA TAGACCTGGG AGTGGGCCTG GCCCCATATT CAGCCGTGGA GAAGGCTATG
    GCGCGGCTTC AGGAACTGCT CACAGTATCA GAGCACTGGG ATGACAAAGC CAAGAGTCTC
    CTCAAGGCCA GGCCTCGGCA TTCACTGAAC AGCCTCGCCG CCGTGGTGAA GGAGATTGAG
    GAGATCCCCG CACACCTGCC CAACGGCGCG GCGCTGAAAG ACTCTGTGCA GAGAGCCAGA
    GACTGGCTTC AGGAAGTGGA GGCCCTGCAG GCTGGAGGAC GTGTGCCAGT GTTGGACACG
    CTGGTGGAGC TTGTCACTCG AGGCCGGTGT ATCCCGGTGC ATCTACCCTC GCTGCCGAGA
    CTGGAGTCCC TAGTAGCTGA AGTTCACGTT TGGAAAGAAT GTGCTGCTAA TACATTCCTG
    ACTAAGAACT CGTCCTATTC TCTCCTAGAG GTGCTGTGTC CTCGATGTGA TATTGGCCTT
    TTGGGATTAA AGAGGAAGCA GAGAAAGTTA AAGGATCCCT TGCCAAGTGG CAAGAAGAAG
    AGTCCCCGTG TAGAGAGTCT GAGCGACCTG GAGAGAACGC TGATGGAAAG CAGAGAGACT
    GCTTCAGCTA TGGCAGCACT CGGGGAAGCT CGCGCACGAG AAATGGAAGC CTTGCAGTCT
    CTCCGACAAG CCAATGAGGA GAAACTGCTG TCGCCTGTGC ACGATGTGGA GATGAGAGTC
    TGCGTGTGTC AGAAGGCTCC CGCGGCCCCC ATGGTCCAGT GTGAACTCTG CAGGGATGCT
    TTCCACACCA GCTGTGTGGC AGCTCCCAGC ATTCCACAGG GCCCCCGAGT CTGGCTGTGT
    CCCAACTGTC GGAGGTCGGA GAAGCCTCCA CTCGAGAAGA TTCTGCCCCT GCTGGCCTCC
    CTGCAGCGCA TCCGCGTCCG CCTGCCCGAG GGAGACGCAC TGCGGTATAT GATCGAGAGG
    ACCGTGAGCT GGCAGCACAG AGCCCGGCAG CTGCTCTCGT CAGGGCACCT CAAGTCCTTG
    CAGGACCCAG TGGGCTCAGG ACTGTTGTGT GGCAGGTGGC AGGCCACAGC AGGACAGGTG
    CCAGAGACCA GCAAGATGTC TCAGCCTCCT GGCCCCACGT CCTTCTCCTT GCCCGACGAC
    TGGGACAACA GAACCTCCTA TTTACACTCT CCTTTCTCCA CTGGTCGGAG CTGTATCCCC
    CTCCATGGTA TCAGCCCAGA AGTGAATGAG CTGCTGATGG AGGCCCAGCT GCTCCAGGTG
    TCCCTTCCTG AAATTCAGGA ACTCTACCAG ACGTTACTTG CAAAGCCGAG CCCTGCGCAG
    CAGGCTGACC ACAGCTCTCC AGTGAGACCC GGCAGTGAGA AGAGTGACGG CTGCCGAGGG
    AAGCGAGATG GAGCCAGCAG CCTCGAGAGC AAGCTGAAGA GACGCCTGGA AAGAGAAGGC
    CTCTCCAGTG AGCGGTGGGA TCGAATCACC AAAATGCGGG CCCCCAAAAA GAAGAGAATC
    AAACTGAGCC ACCCCAAGGA CACGGACAGT CTCAAGTTAG AGAGAGAGTG TAGCTATGAG
    TTGGCCCCCT CTGCCGACAC GCAGTCCCTG CCCTCAGACT CGTCCTCCTC GGAGCAGGAG
    GACTCTGAGG ACGAGGACGC CATCTGCCCG GCCGTGAGCT GCCTGCAGCC AGAAGGAGAT
    GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAGTCGGT GGTTCCATCA GGTCTGTGTT
    GGTGTCTCTC CCGAGACGGC CGAGGAGGAG GACTACGTGT GCGTGCGCTG TGCGGAGACG
    GACGTGCCGG GCCGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330692.1
    CDS620..4780
    Translation

    Target ORF information:

    RefSeq Version XM_015475206.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGTGTCTGCA GAAGCCAAAC
    CTGAGCACAG ACACAAAGGA CAAGGAGTAC AAACCCCATG GTATTCCCCA GAGGCAGTCT
    GTGCAGCCCG CGGAAACCTG CCCCCCAGCC CGACGAGCAA AACGCATGAG AGCGGAGGCC
    ATGAATATTA AAATAGAACC TGAAGAGACA ACAGAAGCCA GAACTCATAA CCTGAGACGT
    CGAATGGGAT GTCCAGCTCC AAAGTGTGAG AATGACAAAG ACGTGAAAAG CAGCGTCAAG
    CAAGAACCTG TTGAAAAGAA AGAGTGTGTT ACAGAAAGTG AGAAGGAAAA ACCCAAGAGT
    CGAGCTAAAA AAACTACCAA TGCTGTTGAC CTGTACGTCT GCCTCTTGTG TGGCAGTGGC
    AGCGACGAGG ACCGGCTTCT CCTGTGCGAT GGGTGTGATG ACAGCTACCA CACCTTCTGC
    CTGATCCCGC CGCTCCATGA CGTTCCCAAG GGAGACTGGA GGTGTCCTAA GTGTTTGGCT
    CAGGAATGCA GTAAGCCACA AGAAGCATTC GGCTTTGAGC AGGCGGCCAG GGATTATACT
    CTCCGTACTT TTGGTGAAAT GGCAGATGCA TTCAAATCTG ATTACTTCAA CATGCCAGTC
    CATATGGTTC CCACGGAACT GGTAGAGAAG GAATTTTGGC GACTAGTGAG CACTATTGAA
    GAGGATGTCA CCGTGGAATA TGGAGCCGAT ATTGCCTCGA AGGAGTTCGG CAGTGGCTTC
    CCTGTCCGAG ATGGGAAAGT CAAGCTCTCA CCTGAGGAGG AGGAGTATCT TGATAGTGGC
    TGGAATCTGA ACAACATGCC AGTGATGGAG CAGTCTGTTC TTGCTCACAT CACTGCCGAC
    ATATGCGGCA TGAAGCTTCC TTGGCTGTAC GTGGGAATGT GCTTTTCGTC CTTCTGTTGG
    CACATTGAAG ACCACTGGAG CTATTCAATT AACTACCTGC ACTGGGGTGA GCCAAAGACC
    TGGTACGGCG TTCCCGGGTA CGCTGCTGAG CAGCTGGAGA CCGTGATGAA GAAGCTGGCC
    CCAGAGCTCT TCATCTCCCA GCCCGACCTT CTCCACCAGC TTGTCACCAT CATGAACCCC
    AACACCCTGA TGACTCACGA AGTGCCTGTT TACCGAACCA ATCAGTGTGC TGGGGAGTTT
    GTGATCACGT TTCCAAGAGC CTACCACAGC GGTTTCAATC AAGGTTTCAA TTTTGCTGAG
    GCCGTTAACT TCTGCACTGT TGATTGGCTG CCCTTAGGCC GGCAGTGCGT GGAGCATTAC
    CGTCTGCTCC ACCGCTACTG CGTGTTCTCC CACGACGAGA TGATCTGCAA GATGGCTTCC
    AAGGCGGATG TCCTAGACGT TGTAGTGGCC TCAACTGTTC AGAAAGACAT GGCCATCATG
    ATTGAGGACG AGAAGGCACT CAGGGAAACT GTTCGCAAAT TGGGAGTAAT TGATTCGGAA
    AGAATGGATT TTGAACTGTT GCCGGATGAT GAGCGTCAGT GTGTGAAATG CAAAACCACG
    TGCTTCATGT CTGCCCTCTC CTGCTGCTGC AAACCTGGCC TGCTTGTTTG CCTGCATCAC
    GTACAGGAGT TATGTTCCTG TCCTCCTTAT AAATACAAAC TGAGGTACAG ATACACTCTG
    GATGACCTCT ACCCCATGAT GAATGCACTG AAGCTTCGAG CAGAGTCTTA TAACGAGTGG
    GCCTTGAATG TGAATGAAGC TTTGGAGGCT AAGATTAATA AGAAGAAAAG CCTTGTCAGC
    TTTAAGGCTT TGATTGAAGA ATCTGAGATG AAGAAATTTC CAGACAACGA TCTTCTACGT
    CACCTCCGCT TAGTCACACA GGACGCGGAG AAATGCGCCT CTGTTGCCCA GCAGCTACTT
    AATGGAAAGA GGCAGACGAG ATACCGGTCT GGTGGAGGGA AATCCCACAA TCAGCTGACC
    GTGAATGAGC TCCGGCAGTT TGTGACCCAG CTGCACGCCC TCCCCTGTGT CCTCAGTCAG
    ACACCGCTGC TGAAGGATCT CTTGAATCGT GTAGAAGATT TCCAACAGCA TAGCCAGAAA
    CTGCTCTCTG AGGAGATGCC CAGTGCTTCT GAGCTGCAGG ACTTGCTGGA TGTCAGCTTT
    GAATTTGATG TTGAGCTCCC CCAGCTTGCT GAGATGCGTG TCCGGCTGGA GCAGGCCCAT
    TGGCTGGAGG AGGTGCAGCA GGCTTGCCTG GACCCGAGCT CCCTCACCTT AGATGATATG
    AGACGTCTCA TAGACCTGGG AGTGGGCCTG GCCCCATATT CAGCCGTGGA GAAGGCTATG
    GCGCGGCTTC AGGAACTGCT CACAGTATCA GAGCACTGGG ATGACAAAGC CAAGAGTCTC
    CTCAAGGCCA GGCCTCGGCA TTCACTGAAC AGCCTCGCCG CCGTGGTGAA GGAGATTGAG
    GAGATCCCCG CACACCTGCC CAACGGCGCG GCGCTGAAAG ACTCTGTGCA GAGAGCCAGA
    GACTGGCTTC AGGAAGTGGA GGCCCTGCAG GCTGGAGGAC GTGTGCCAGT GTTGGACACG
    CTGGTGGAGC TTGTCACTCG AGGCCGGTGT ATCCCGGTGC ATCTACCCTC GCTGCCGAGA
    CTGGAGTCCC TAGTAGCTGA AGTTCACGTT TGGAAAGAAT GTGCTGCTAA TACATTCCTG
    ACTAAGAACT CGTCCTATTC TCTCCTAGAG GTGCTGTGTC CTCGATGTGA TATTGGCCTT
    TTGGGATTAA AGAGGAAGCA GAGAAAGTTA AAGGATCCCT TGCCAAGTGG CAAGAAGAAG
    AGTCCCCGTG TAGAGAGTCT GAGCGACCTG GAGAGAACGC TGATGGAAAG CAGAGAGACT
    GCTTCAGCTA TGGCAGCACT CGGGGAAGCT CGCGCACGAG AAATGGAAGC CTTGCAGTCT
    CTCCGACAAG CCAATGAGGA GAAACTGCTG TCGCCTGTGC ACGATGTGGA GATGAGAGTC
    TGCGTGTGTC AGAAGGCTCC CGCGGCCCCC ATGGTCCAGT GTGAACTCTG CAGGGATGCT
    TTCCACACCA GCTGTGTGGC AGCTCCCAGC ATTCCACAGG GCCCCCGAGT CTGGCTGTGT
    CCCAACTGTC GGAGGTCGGA GAAGCCTCCA CTCGAGAAGA TTCTGCCCCT GCTGGCCTCC
    CTGCAGCGCA TCCGCGTCCG CCTGCCCGAG GGAGACGCAC TGCGGTATAT GATCGAGAGG
    ACCGTGAGCT GGCAGCACAG AGCCCGGCAG CTGCTCTCGT CAGGGCACCT CAAGTCCTTG
    CAGGACCCAG TGGGCTCAGG ACTGTTGTGT GGCAGGTGGC AGGCCACAGC AGGACAGGTG
    CCAGAGACCA GCAAGATGTC TCAGCCTCCT GGCCCCACGT CCTTCTCCTT GCCCGACGAC
    TGGGACAACA GAACCTCCTA TTTACACTCT CCTTTCTCCA CTGGTCGGAG CTGTATCCCC
    CTCCATGGTA TCAGCCCAGA AGTGAATGAG CTGCTGATGG AGGCCCAGCT GCTCCAGGTG
    TCCCTTCCTG AAATTCAGGA ACTCTACCAG ACGTTACTTG CAAAGCCGAG CCCTGCGCAG
    CAGGCTGACC ACAGCTCTCC AGTGAGACCC GGCAGTGAGA AGAGTGACGG CTGCCGAGGG
    AAGCGAGATG GAGCCAGCAG CCTCGAGAGC AAGCTGAAGA GACGCCTGGA AAGAGAAGGC
    CTCTCCAGTG AGCGGTGGGA TCGAATCACC AAAATGCGGG CCCCCAAAAA GAAGAGAATC
    AAACTGAGCC ACCCCAAGGA CACGGACAGT CTCAAGTTAG AGAGAGAGTG TAGCTATGAG
    TTGGCCCCCT CTGCCGACAC GCAGTCCCTG CCCTCAGACT CGTCCTCCTC GGAGCAGGAG
    GACTCTGAGG ACGAGGACGC CATCTGCCCG GCCGTGAGCT GCCTGCAGCC AGAAGGAGAT
    GAGGTGGACT GGGTCCAGTG TGATGGCAGC TGCAGTCGGT GGTTCCATCA GGTCTGTGTT
    GGTGTCTCTC CCGAGACGGC CGAGGAGGAG GACTACGTGT GCGTGCGCTG TGCGGAGACG
    GACGTGCCGG GCCGGAGATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93015
    Clone ID Related Accession (Same CDS sequence) XM_015466902.1 , XM_015475207.1
    Accession Version XM_015466902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGGTTGACCT GTACGTCTGC
    CTCTTGTGTG GCAGTGGCAG CGACGAGGAC CGGCTTCTCC TGTGCGATGG GTGTGATGAC
    AGCTACCACA CCTTCTGCCT GATCCCGCCG CTCCATGACG TTCCCAAGGG AGACTGGAGG
    TGTCCTAAGT GTTTGGCTCA GGAATGCAGT AAGCCACAAG AAGCATTCGG CTTTGAGCAG
    GCGGCCAGGG ATTATACTCT CCGTACTTTT GGTGAAATGG CAGATGCATT CAAATCTGAT
    TACTTCAACA TGCCAGTCCA TATGGTTCCC ACGGAACTGG TAGAGAAGGA ATTTTGGCGA
    CTAGTGAGCA CTATTGAAGA GGATGTCACC GTGGAATATG GAGCCGATAT TGCCTCGAAG
    GAGTTCGGCA GTGGCTTCCC TGTCCGAGAT GGGAAAGTCA AGCTCTCACC TGAGGAGGAG
    GAGTATCTTG ATAGTGGCTG GAATCTGAAC AACATGCCAG TGATGGAGCA GTCTGTTCTT
    GCTCACATCA CTGCCGACAT ATGCGGCATG AAGCTTCCTT GGCTGTACGT GGGAATGTGC
    TTTTCGTCCT TCTGTTGGCA CATTGAAGAC CACTGGAGCT ATTCAATTAA CTACCTGCAC
    TGGGGTGAGC CAAAGACCTG GTACGGCGTT CCCGGGTACG CTGCTGAGCA GCTGGAGACC
    GTGATGAAGA AGCTGGCCCC AGAGCTCTTC ATCTCCCAGC CCGACCTTCT CCACCAGCTT
    GTCACCATCA TGAACCCCAA CACCCTGATG ACTCACGAAG TGCCTGTTTA CCGAACCAAT
    CAGTGTGCTG GGGAGTTTGT GATCACGTTT CCAAGAGCCT ACCACAGCGG TTTCAATCAA
    GGTTTCAATT TTGCTGAGGC CGTTAACTTC TGCACTGTTG ATTGGCTGCC CTTAGGCCGG
    CAGTGCGTGG AGCATTACCG TCTGCTCCAC CGCTACTGCG TGTTCTCCCA CGACGAGATG
    ATCTGCAAGA TGGCTTCCAA GGCGGATGTC CTAGACGTTG TAGTGGCCTC AACTGTTCAG
    AAAGACATGG CCATCATGAT TGAGGACGAG AAGGCACTCA GGGAAACTGT TCGCAAATTG
    GGAGTAATTG ATTCGGAAAG AATGGATTTT GAACTGTTGC CGGATGATGA GCGTCAGTGT
    GTGAAATGCA AAACCACGTG CTTCATGTCT GCCCTCTCCT GCTGCTGCAA ACCTGGCCTG
    CTTGTTTGCC TGCATCACGT ACAGGAGTTA TGTTCCTGTC CTCCTTATAA ATACAAACTG
    AGGTACAGAT ACACTCTGGA TGACCTCTAC CCCATGATGA ATGCACTGAA GCTTCGAGCA
    GAGTCTTATA ACGAGTGGGC CTTGAATGTG AATGAAGCTT TGGAGGCTAA GATTAATAAG
    AAGAAAAGCC TTGTCAGCTT TAAGGCTTTG ATTGAAGAAT CTGAGATGAA GAAATTTCCA
    GACAACGATC TTCTACGTCA CCTCCGCTTA GTCACACAGG ACGCGGAGAA ATGCGCCTCT
    GTTGCCCAGC AGCTACTTAA TGGAAAGAGG CAGACGAGAT ACCGGTCTGG TGGAGGGAAA
    TCCCACAATC AGCTGACCGT GAATGAGCTC CGGCAGTTTG TGACCCAGCT GCACGCCCTC
    CCCTGTGTCC TCAGTCAGAC ACCGCTGCTG AAGGATCTCT TGAATCGTGT AGAAGATTTC
    CAACAGCATA GCCAGAAACT GCTCTCTGAG GAGATGCCCA GTGCTTCTGA GCTGCAGGAC
    TTGCTGGATG TCAGCTTTGA ATTTGATGTT GAGCTCCCCC AGCTTGCTGA GATGCGTGTC
    CGGCTGGAGC AGGCCCATTG GCTGGAGGAG GTGCAGCAGG CTTGCCTGGA CCCGAGCTCC
    CTCACCTTAG ATGATATGAG ACGTCTCATA GACCTGGGAG TGGGCCTGGC CCCATATTCA
    GCCGTGGAGA AGGCTATGGC GCGGCTTCAG GAACTGCTCA CAGTATCAGA GCACTGGGAT
    GACAAAGCCA AGAGTCTCCT CAAGGCCAGG CCTCGGCATT CACTGAACAG CCTCGCCGCC
    GTGGTGAAGG AGATTGAGGA GATCCCCGCA CACCTGCCCA ACGGCGCGGC GCTGAAAGAC
    TCTGTGCAGA GAGCCAGAGA CTGGCTTCAG GAAGTGGAGG CCCTGCAGGC TGGAGGACGT
    GTGCCAGTGT TGGACACGCT GGTGGAGCTT GTCACTCGAG GCCGGTGTAT CCCGGTGCAT
    CTACCCTCGC TGCCGAGACT GGAGTCCCTA GTAGCTGAAG TTCACGTTTG GAAAGAATGT
    GCTGCTAATA CATTCCTGAC TAAGAACTCG TCCTATTCTC TCCTAGAGGT GCTGTGTCCT
    CGATGTGATA TTGGCCTTTT GGGATTAAAG AGGAAGCAGA GAAAGTTAAA GGATCCCTTG
    CCAAGTGGCA AGAAGAAGAG TCCCCGTGTA GAGAGTCTGA GCGACCTGGA GAGAACGCTG
    ATGGAAAGCA GAGAGACTGC TTCAGCTATG GCAGCACTCG GGGAAGCTCG CGCACGAGAA
    ATGGAAGCCT TGCAGTCTCT CCGACAAGCC AATGAGGAGA AACTGCTGTC GCCTGTGCAC
    GATGTGGAGA TGAGAGTCTG CGTGTGTCAG AAGGCTCCCG CGGCCCCCAT GGTCCAGTGT
    GAACTCTGCA GGGATGCTTT CCACACCAGC TGTGTGGCAG CTCCCAGCAT TCCACAGGGC
    CCCCGAGTCT GGCTGTGTCC CAACTGTCGG AGGTCGGAGA AGCCTCCACT CGAGAAGATT
    CTGCCCCTGC TGGCCTCCCT GCAGCGCATC CGCGTCCGCC TGCCCGAGGG AGACGCACTG
    CGGTATATGA TCGAGAGGAC CGTGAGCTGG CAGCACAGAG CCCGGCAGCT GCTCTCGTCA
    GGGCACCTCA AGTCCTTGCA GGACCCAGTG GGCTCAGGAC TGTTGTGTGG CAGGTGGCAG
    GCCACAGCAG GACAGGTGCC AGAGACCAGC AAGATGTCTC AGCCTCCTGG CCCCACGTCC
    TTCTCCTTGC CCGACGACTG GGACAACAGA ACCTCCTATT TACACTCTCC TTTCTCCACT
    GGTCGGAGCT GTATCCCCCT CCATGGTATC AGCCCAGAAG TGAATGAGCT GCTGATGGAG
    GCCCAGCTGC TCCAGGTGTC CCTTCCTGAA ATTCAGGAAC TCTACCAGAC GTTACTTGCA
    AAGCCGAGCC CTGCGCAGCA GGCTGACCAC AGCTCTCCAG TGAGACCCGG CAGTGAGAAG
    AGTGACGGCT GCCGAGGGAA GCGAGATGGA GCCAGCAGCC TCGAGAGCAA GCTGAAGAGA
    CGCCTGGAAA GAGAAGGCCT CTCCAGTGAG CGGTGGGATC GAATCACCAA AATGCGGGCC
    CCCAAAAAGA AGAGAATCAA ACTGAGCCAC CCCAAGGACA CGGACAGTCT CAAGTTAGAG
    AGAGAGTGTA GCTATGAGTT GGCCCCCTCT GCCGACACGC AGTCCCTGCC CTCAGACTCG
    TCCTCCTCGG AGCAGGAGGA CTCTGAGGAC GAGGACGCCA TCTGCCCGGC CGTGAGCTGC
    CTGCAGCCAG AAGGAGATGA GGTGGACTGG GTCCAGTGTG ATGGCAGCTG CAGTCGGTGG
    TTCCATCAGG TCTGTGTTGG TGTCTCTCCC GAGACGGCCG AGGAGGAGGA CTACGTGTGC
    GTGCGCTGTG CGGAGACGGA CGTGCCGGGC CGGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322388.1
    CDS621..4439
    Translation

    Target ORF information:

    RefSeq Version XM_015466902.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGGTTGACCT GTACGTCTGC
    CTCTTGTGTG GCAGTGGCAG CGACGAGGAC CGGCTTCTCC TGTGCGATGG GTGTGATGAC
    AGCTACCACA CCTTCTGCCT GATCCCGCCG CTCCATGACG TTCCCAAGGG AGACTGGAGG
    TGTCCTAAGT GTTTGGCTCA GGAATGCAGT AAGCCACAAG AAGCATTCGG CTTTGAGCAG
    GCGGCCAGGG ATTATACTCT CCGTACTTTT GGTGAAATGG CAGATGCATT CAAATCTGAT
    TACTTCAACA TGCCAGTCCA TATGGTTCCC ACGGAACTGG TAGAGAAGGA ATTTTGGCGA
    CTAGTGAGCA CTATTGAAGA GGATGTCACC GTGGAATATG GAGCCGATAT TGCCTCGAAG
    GAGTTCGGCA GTGGCTTCCC TGTCCGAGAT GGGAAAGTCA AGCTCTCACC TGAGGAGGAG
    GAGTATCTTG ATAGTGGCTG GAATCTGAAC AACATGCCAG TGATGGAGCA GTCTGTTCTT
    GCTCACATCA CTGCCGACAT ATGCGGCATG AAGCTTCCTT GGCTGTACGT GGGAATGTGC
    TTTTCGTCCT TCTGTTGGCA CATTGAAGAC CACTGGAGCT ATTCAATTAA CTACCTGCAC
    TGGGGTGAGC CAAAGACCTG GTACGGCGTT CCCGGGTACG CTGCTGAGCA GCTGGAGACC
    GTGATGAAGA AGCTGGCCCC AGAGCTCTTC ATCTCCCAGC CCGACCTTCT CCACCAGCTT
    GTCACCATCA TGAACCCCAA CACCCTGATG ACTCACGAAG TGCCTGTTTA CCGAACCAAT
    CAGTGTGCTG GGGAGTTTGT GATCACGTTT CCAAGAGCCT ACCACAGCGG TTTCAATCAA
    GGTTTCAATT TTGCTGAGGC CGTTAACTTC TGCACTGTTG ATTGGCTGCC CTTAGGCCGG
    CAGTGCGTGG AGCATTACCG TCTGCTCCAC CGCTACTGCG TGTTCTCCCA CGACGAGATG
    ATCTGCAAGA TGGCTTCCAA GGCGGATGTC CTAGACGTTG TAGTGGCCTC AACTGTTCAG
    AAAGACATGG CCATCATGAT TGAGGACGAG AAGGCACTCA GGGAAACTGT TCGCAAATTG
    GGAGTAATTG ATTCGGAAAG AATGGATTTT GAACTGTTGC CGGATGATGA GCGTCAGTGT
    GTGAAATGCA AAACCACGTG CTTCATGTCT GCCCTCTCCT GCTGCTGCAA ACCTGGCCTG
    CTTGTTTGCC TGCATCACGT ACAGGAGTTA TGTTCCTGTC CTCCTTATAA ATACAAACTG
    AGGTACAGAT ACACTCTGGA TGACCTCTAC CCCATGATGA ATGCACTGAA GCTTCGAGCA
    GAGTCTTATA ACGAGTGGGC CTTGAATGTG AATGAAGCTT TGGAGGCTAA GATTAATAAG
    AAGAAAAGCC TTGTCAGCTT TAAGGCTTTG ATTGAAGAAT CTGAGATGAA GAAATTTCCA
    GACAACGATC TTCTACGTCA CCTCCGCTTA GTCACACAGG ACGCGGAGAA ATGCGCCTCT
    GTTGCCCAGC AGCTACTTAA TGGAAAGAGG CAGACGAGAT ACCGGTCTGG TGGAGGGAAA
    TCCCACAATC AGCTGACCGT GAATGAGCTC CGGCAGTTTG TGACCCAGCT GCACGCCCTC
    CCCTGTGTCC TCAGTCAGAC ACCGCTGCTG AAGGATCTCT TGAATCGTGT AGAAGATTTC
    CAACAGCATA GCCAGAAACT GCTCTCTGAG GAGATGCCCA GTGCTTCTGA GCTGCAGGAC
    TTGCTGGATG TCAGCTTTGA ATTTGATGTT GAGCTCCCCC AGCTTGCTGA GATGCGTGTC
    CGGCTGGAGC AGGCCCATTG GCTGGAGGAG GTGCAGCAGG CTTGCCTGGA CCCGAGCTCC
    CTCACCTTAG ATGATATGAG ACGTCTCATA GACCTGGGAG TGGGCCTGGC CCCATATTCA
    GCCGTGGAGA AGGCTATGGC GCGGCTTCAG GAACTGCTCA CAGTATCAGA GCACTGGGAT
    GACAAAGCCA AGAGTCTCCT CAAGGCCAGG CCTCGGCATT CACTGAACAG CCTCGCCGCC
    GTGGTGAAGG AGATTGAGGA GATCCCCGCA CACCTGCCCA ACGGCGCGGC GCTGAAAGAC
    TCTGTGCAGA GAGCCAGAGA CTGGCTTCAG GAAGTGGAGG CCCTGCAGGC TGGAGGACGT
    GTGCCAGTGT TGGACACGCT GGTGGAGCTT GTCACTCGAG GCCGGTGTAT CCCGGTGCAT
    CTACCCTCGC TGCCGAGACT GGAGTCCCTA GTAGCTGAAG TTCACGTTTG GAAAGAATGT
    GCTGCTAATA CATTCCTGAC TAAGAACTCG TCCTATTCTC TCCTAGAGGT GCTGTGTCCT
    CGATGTGATA TTGGCCTTTT GGGATTAAAG AGGAAGCAGA GAAAGTTAAA GGATCCCTTG
    CCAAGTGGCA AGAAGAAGAG TCCCCGTGTA GAGAGTCTGA GCGACCTGGA GAGAACGCTG
    ATGGAAAGCA GAGAGACTGC TTCAGCTATG GCAGCACTCG GGGAAGCTCG CGCACGAGAA
    ATGGAAGCCT TGCAGTCTCT CCGACAAGCC AATGAGGAGA AACTGCTGTC GCCTGTGCAC
    GATGTGGAGA TGAGAGTCTG CGTGTGTCAG AAGGCTCCCG CGGCCCCCAT GGTCCAGTGT
    GAACTCTGCA GGGATGCTTT CCACACCAGC TGTGTGGCAG CTCCCAGCAT TCCACAGGGC
    CCCCGAGTCT GGCTGTGTCC CAACTGTCGG AGGTCGGAGA AGCCTCCACT CGAGAAGATT
    CTGCCCCTGC TGGCCTCCCT GCAGCGCATC CGCGTCCGCC TGCCCGAGGG AGACGCACTG
    CGGTATATGA TCGAGAGGAC CGTGAGCTGG CAGCACAGAG CCCGGCAGCT GCTCTCGTCA
    GGGCACCTCA AGTCCTTGCA GGACCCAGTG GGCTCAGGAC TGTTGTGTGG CAGGTGGCAG
    GCCACAGCAG GACAGGTGCC AGAGACCAGC AAGATGTCTC AGCCTCCTGG CCCCACGTCC
    TTCTCCTTGC CCGACGACTG GGACAACAGA ACCTCCTATT TACACTCTCC TTTCTCCACT
    GGTCGGAGCT GTATCCCCCT CCATGGTATC AGCCCAGAAG TGAATGAGCT GCTGATGGAG
    GCCCAGCTGC TCCAGGTGTC CCTTCCTGAA ATTCAGGAAC TCTACCAGAC GTTACTTGCA
    AAGCCGAGCC CTGCGCAGCA GGCTGACCAC AGCTCTCCAG TGAGACCCGG CAGTGAGAAG
    AGTGACGGCT GCCGAGGGAA GCGAGATGGA GCCAGCAGCC TCGAGAGCAA GCTGAAGAGA
    CGCCTGGAAA GAGAAGGCCT CTCCAGTGAG CGGTGGGATC GAATCACCAA AATGCGGGCC
    CCCAAAAAGA AGAGAATCAA ACTGAGCCAC CCCAAGGACA CGGACAGTCT CAAGTTAGAG
    AGAGAGTGTA GCTATGAGTT GGCCCCCTCT GCCGACACGC AGTCCCTGCC CTCAGACTCG
    TCCTCCTCGG AGCAGGAGGA CTCTGAGGAC GAGGACGCCA TCTGCCCGGC CGTGAGCTGC
    CTGCAGCCAG AAGGAGATGA GGTGGACTGG GTCCAGTGTG ATGGCAGCTG CAGTCGGTGG
    TTCCATCAGG TCTGTGTTGG TGTCTCTCCC GAGACGGCCG AGGAGGAGGA CTACGTGTGC
    GTGCGCTGTG CGGAGACGGA CGTGCCGGGC CGGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93015
    Clone ID Related Accession (Same CDS sequence) XM_015466902.1 , XM_015475207.1
    Accession Version XM_015475207.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGGTTGACCT GTACGTCTGC
    CTCTTGTGTG GCAGTGGCAG CGACGAGGAC CGGCTTCTCC TGTGCGATGG GTGTGATGAC
    AGCTACCACA CCTTCTGCCT GATCCCGCCG CTCCATGACG TTCCCAAGGG AGACTGGAGG
    TGTCCTAAGT GTTTGGCTCA GGAATGCAGT AAGCCACAAG AAGCATTCGG CTTTGAGCAG
    GCGGCCAGGG ATTATACTCT CCGTACTTTT GGTGAAATGG CAGATGCATT CAAATCTGAT
    TACTTCAACA TGCCAGTCCA TATGGTTCCC ACGGAACTGG TAGAGAAGGA ATTTTGGCGA
    CTAGTGAGCA CTATTGAAGA GGATGTCACC GTGGAATATG GAGCCGATAT TGCCTCGAAG
    GAGTTCGGCA GTGGCTTCCC TGTCCGAGAT GGGAAAGTCA AGCTCTCACC TGAGGAGGAG
    GAGTATCTTG ATAGTGGCTG GAATCTGAAC AACATGCCAG TGATGGAGCA GTCTGTTCTT
    GCTCACATCA CTGCCGACAT ATGCGGCATG AAGCTTCCTT GGCTGTACGT GGGAATGTGC
    TTTTCGTCCT TCTGTTGGCA CATTGAAGAC CACTGGAGCT ATTCAATTAA CTACCTGCAC
    TGGGGTGAGC CAAAGACCTG GTACGGCGTT CCCGGGTACG CTGCTGAGCA GCTGGAGACC
    GTGATGAAGA AGCTGGCCCC AGAGCTCTTC ATCTCCCAGC CCGACCTTCT CCACCAGCTT
    GTCACCATCA TGAACCCCAA CACCCTGATG ACTCACGAAG TGCCTGTTTA CCGAACCAAT
    CAGTGTGCTG GGGAGTTTGT GATCACGTTT CCAAGAGCCT ACCACAGCGG TTTCAATCAA
    GGTTTCAATT TTGCTGAGGC CGTTAACTTC TGCACTGTTG ATTGGCTGCC CTTAGGCCGG
    CAGTGCGTGG AGCATTACCG TCTGCTCCAC CGCTACTGCG TGTTCTCCCA CGACGAGATG
    ATCTGCAAGA TGGCTTCCAA GGCGGATGTC CTAGACGTTG TAGTGGCCTC AACTGTTCAG
    AAAGACATGG CCATCATGAT TGAGGACGAG AAGGCACTCA GGGAAACTGT TCGCAAATTG
    GGAGTAATTG ATTCGGAAAG AATGGATTTT GAACTGTTGC CGGATGATGA GCGTCAGTGT
    GTGAAATGCA AAACCACGTG CTTCATGTCT GCCCTCTCCT GCTGCTGCAA ACCTGGCCTG
    CTTGTTTGCC TGCATCACGT ACAGGAGTTA TGTTCCTGTC CTCCTTATAA ATACAAACTG
    AGGTACAGAT ACACTCTGGA TGACCTCTAC CCCATGATGA ATGCACTGAA GCTTCGAGCA
    GAGTCTTATA ACGAGTGGGC CTTGAATGTG AATGAAGCTT TGGAGGCTAA GATTAATAAG
    AAGAAAAGCC TTGTCAGCTT TAAGGCTTTG ATTGAAGAAT CTGAGATGAA GAAATTTCCA
    GACAACGATC TTCTACGTCA CCTCCGCTTA GTCACACAGG ACGCGGAGAA ATGCGCCTCT
    GTTGCCCAGC AGCTACTTAA TGGAAAGAGG CAGACGAGAT ACCGGTCTGG TGGAGGGAAA
    TCCCACAATC AGCTGACCGT GAATGAGCTC CGGCAGTTTG TGACCCAGCT GCACGCCCTC
    CCCTGTGTCC TCAGTCAGAC ACCGCTGCTG AAGGATCTCT TGAATCGTGT AGAAGATTTC
    CAACAGCATA GCCAGAAACT GCTCTCTGAG GAGATGCCCA GTGCTTCTGA GCTGCAGGAC
    TTGCTGGATG TCAGCTTTGA ATTTGATGTT GAGCTCCCCC AGCTTGCTGA GATGCGTGTC
    CGGCTGGAGC AGGCCCATTG GCTGGAGGAG GTGCAGCAGG CTTGCCTGGA CCCGAGCTCC
    CTCACCTTAG ATGATATGAG ACGTCTCATA GACCTGGGAG TGGGCCTGGC CCCATATTCA
    GCCGTGGAGA AGGCTATGGC GCGGCTTCAG GAACTGCTCA CAGTATCAGA GCACTGGGAT
    GACAAAGCCA AGAGTCTCCT CAAGGCCAGG CCTCGGCATT CACTGAACAG CCTCGCCGCC
    GTGGTGAAGG AGATTGAGGA GATCCCCGCA CACCTGCCCA ACGGCGCGGC GCTGAAAGAC
    TCTGTGCAGA GAGCCAGAGA CTGGCTTCAG GAAGTGGAGG CCCTGCAGGC TGGAGGACGT
    GTGCCAGTGT TGGACACGCT GGTGGAGCTT GTCACTCGAG GCCGGTGTAT CCCGGTGCAT
    CTACCCTCGC TGCCGAGACT GGAGTCCCTA GTAGCTGAAG TTCACGTTTG GAAAGAATGT
    GCTGCTAATA CATTCCTGAC TAAGAACTCG TCCTATTCTC TCCTAGAGGT GCTGTGTCCT
    CGATGTGATA TTGGCCTTTT GGGATTAAAG AGGAAGCAGA GAAAGTTAAA GGATCCCTTG
    CCAAGTGGCA AGAAGAAGAG TCCCCGTGTA GAGAGTCTGA GCGACCTGGA GAGAACGCTG
    ATGGAAAGCA GAGAGACTGC TTCAGCTATG GCAGCACTCG GGGAAGCTCG CGCACGAGAA
    ATGGAAGCCT TGCAGTCTCT CCGACAAGCC AATGAGGAGA AACTGCTGTC GCCTGTGCAC
    GATGTGGAGA TGAGAGTCTG CGTGTGTCAG AAGGCTCCCG CGGCCCCCAT GGTCCAGTGT
    GAACTCTGCA GGGATGCTTT CCACACCAGC TGTGTGGCAG CTCCCAGCAT TCCACAGGGC
    CCCCGAGTCT GGCTGTGTCC CAACTGTCGG AGGTCGGAGA AGCCTCCACT CGAGAAGATT
    CTGCCCCTGC TGGCCTCCCT GCAGCGCATC CGCGTCCGCC TGCCCGAGGG AGACGCACTG
    CGGTATATGA TCGAGAGGAC CGTGAGCTGG CAGCACAGAG CCCGGCAGCT GCTCTCGTCA
    GGGCACCTCA AGTCCTTGCA GGACCCAGTG GGCTCAGGAC TGTTGTGTGG CAGGTGGCAG
    GCCACAGCAG GACAGGTGCC AGAGACCAGC AAGATGTCTC AGCCTCCTGG CCCCACGTCC
    TTCTCCTTGC CCGACGACTG GGACAACAGA ACCTCCTATT TACACTCTCC TTTCTCCACT
    GGTCGGAGCT GTATCCCCCT CCATGGTATC AGCCCAGAAG TGAATGAGCT GCTGATGGAG
    GCCCAGCTGC TCCAGGTGTC CCTTCCTGAA ATTCAGGAAC TCTACCAGAC GTTACTTGCA
    AAGCCGAGCC CTGCGCAGCA GGCTGACCAC AGCTCTCCAG TGAGACCCGG CAGTGAGAAG
    AGTGACGGCT GCCGAGGGAA GCGAGATGGA GCCAGCAGCC TCGAGAGCAA GCTGAAGAGA
    CGCCTGGAAA GAGAAGGCCT CTCCAGTGAG CGGTGGGATC GAATCACCAA AATGCGGGCC
    CCCAAAAAGA AGAGAATCAA ACTGAGCCAC CCCAAGGACA CGGACAGTCT CAAGTTAGAG
    AGAGAGTGTA GCTATGAGTT GGCCCCCTCT GCCGACACGC AGTCCCTGCC CTCAGACTCG
    TCCTCCTCGG AGCAGGAGGA CTCTGAGGAC GAGGACGCCA TCTGCCCGGC CGTGAGCTGC
    CTGCAGCCAG AAGGAGATGA GGTGGACTGG GTCCAGTGTG ATGGCAGCTG CAGTCGGTGG
    TTCCATCAGG TCTGTGTTGG TGTCTCTCCC GAGACGGCCG AGGAGGAGGA CTACGTGTGC
    GTGCGCTGTG CGGAGACGGA CGTGCCGGGC CGGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330693.1
    CDS621..4439
    Translation

    Target ORF information:

    RefSeq Version XM_015475207.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 5B (KDM5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGGCTTTG CTCCTGGCAA AGCTGTGGGC TCTCATATAA GAGGACACTA CGAGCGGATT 
    CTGAACCCCT ACAACTTATT TCTGTCTGGA GACAGCTTGA GGGTTGACCT GTACGTCTGC
    CTCTTGTGTG GCAGTGGCAG CGACGAGGAC CGGCTTCTCC TGTGCGATGG GTGTGATGAC
    AGCTACCACA CCTTCTGCCT GATCCCGCCG CTCCATGACG TTCCCAAGGG AGACTGGAGG
    TGTCCTAAGT GTTTGGCTCA GGAATGCAGT AAGCCACAAG AAGCATTCGG CTTTGAGCAG
    GCGGCCAGGG ATTATACTCT CCGTACTTTT GGTGAAATGG CAGATGCATT CAAATCTGAT
    TACTTCAACA TGCCAGTCCA TATGGTTCCC ACGGAACTGG TAGAGAAGGA ATTTTGGCGA
    CTAGTGAGCA CTATTGAAGA GGATGTCACC GTGGAATATG GAGCCGATAT TGCCTCGAAG
    GAGTTCGGCA GTGGCTTCCC TGTCCGAGAT GGGAAAGTCA AGCTCTCACC TGAGGAGGAG
    GAGTATCTTG ATAGTGGCTG GAATCTGAAC AACATGCCAG TGATGGAGCA GTCTGTTCTT
    GCTCACATCA CTGCCGACAT ATGCGGCATG AAGCTTCCTT GGCTGTACGT GGGAATGTGC
    TTTTCGTCCT TCTGTTGGCA CATTGAAGAC CACTGGAGCT ATTCAATTAA CTACCTGCAC
    TGGGGTGAGC CAAAGACCTG GTACGGCGTT CCCGGGTACG CTGCTGAGCA GCTGGAGACC
    GTGATGAAGA AGCTGGCCCC AGAGCTCTTC ATCTCCCAGC CCGACCTTCT CCACCAGCTT
    GTCACCATCA TGAACCCCAA CACCCTGATG ACTCACGAAG TGCCTGTTTA CCGAACCAAT
    CAGTGTGCTG GGGAGTTTGT GATCACGTTT CCAAGAGCCT ACCACAGCGG TTTCAATCAA
    GGTTTCAATT TTGCTGAGGC CGTTAACTTC TGCACTGTTG ATTGGCTGCC CTTAGGCCGG
    CAGTGCGTGG AGCATTACCG TCTGCTCCAC CGCTACTGCG TGTTCTCCCA CGACGAGATG
    ATCTGCAAGA TGGCTTCCAA GGCGGATGTC CTAGACGTTG TAGTGGCCTC AACTGTTCAG
    AAAGACATGG CCATCATGAT TGAGGACGAG AAGGCACTCA GGGAAACTGT TCGCAAATTG
    GGAGTAATTG ATTCGGAAAG AATGGATTTT GAACTGTTGC CGGATGATGA GCGTCAGTGT
    GTGAAATGCA AAACCACGTG CTTCATGTCT GCCCTCTCCT GCTGCTGCAA ACCTGGCCTG
    CTTGTTTGCC TGCATCACGT ACAGGAGTTA TGTTCCTGTC CTCCTTATAA ATACAAACTG
    AGGTACAGAT ACACTCTGGA TGACCTCTAC CCCATGATGA ATGCACTGAA GCTTCGAGCA
    GAGTCTTATA ACGAGTGGGC CTTGAATGTG AATGAAGCTT TGGAGGCTAA GATTAATAAG
    AAGAAAAGCC TTGTCAGCTT TAAGGCTTTG ATTGAAGAAT CTGAGATGAA GAAATTTCCA
    GACAACGATC TTCTACGTCA CCTCCGCTTA GTCACACAGG ACGCGGAGAA ATGCGCCTCT
    GTTGCCCAGC AGCTACTTAA TGGAAAGAGG CAGACGAGAT ACCGGTCTGG TGGAGGGAAA
    TCCCACAATC AGCTGACCGT GAATGAGCTC CGGCAGTTTG TGACCCAGCT GCACGCCCTC
    CCCTGTGTCC TCAGTCAGAC ACCGCTGCTG AAGGATCTCT TGAATCGTGT AGAAGATTTC
    CAACAGCATA GCCAGAAACT GCTCTCTGAG GAGATGCCCA GTGCTTCTGA GCTGCAGGAC
    TTGCTGGATG TCAGCTTTGA ATTTGATGTT GAGCTCCCCC AGCTTGCTGA GATGCGTGTC
    CGGCTGGAGC AGGCCCATTG GCTGGAGGAG GTGCAGCAGG CTTGCCTGGA CCCGAGCTCC
    CTCACCTTAG ATGATATGAG ACGTCTCATA GACCTGGGAG TGGGCCTGGC CCCATATTCA
    GCCGTGGAGA AGGCTATGGC GCGGCTTCAG GAACTGCTCA CAGTATCAGA GCACTGGGAT
    GACAAAGCCA AGAGTCTCCT CAAGGCCAGG CCTCGGCATT CACTGAACAG CCTCGCCGCC
    GTGGTGAAGG AGATTGAGGA GATCCCCGCA CACCTGCCCA ACGGCGCGGC GCTGAAAGAC
    TCTGTGCAGA GAGCCAGAGA CTGGCTTCAG GAAGTGGAGG CCCTGCAGGC TGGAGGACGT
    GTGCCAGTGT TGGACACGCT GGTGGAGCTT GTCACTCGAG GCCGGTGTAT CCCGGTGCAT
    CTACCCTCGC TGCCGAGACT GGAGTCCCTA GTAGCTGAAG TTCACGTTTG GAAAGAATGT
    GCTGCTAATA CATTCCTGAC TAAGAACTCG TCCTATTCTC TCCTAGAGGT GCTGTGTCCT
    CGATGTGATA TTGGCCTTTT GGGATTAAAG AGGAAGCAGA GAAAGTTAAA GGATCCCTTG
    CCAAGTGGCA AGAAGAAGAG TCCCCGTGTA GAGAGTCTGA GCGACCTGGA GAGAACGCTG
    ATGGAAAGCA GAGAGACTGC TTCAGCTATG GCAGCACTCG GGGAAGCTCG CGCACGAGAA
    ATGGAAGCCT TGCAGTCTCT CCGACAAGCC AATGAGGAGA AACTGCTGTC GCCTGTGCAC
    GATGTGGAGA TGAGAGTCTG CGTGTGTCAG AAGGCTCCCG CGGCCCCCAT GGTCCAGTGT
    GAACTCTGCA GGGATGCTTT CCACACCAGC TGTGTGGCAG CTCCCAGCAT TCCACAGGGC
    CCCCGAGTCT GGCTGTGTCC CAACTGTCGG AGGTCGGAGA AGCCTCCACT CGAGAAGATT
    CTGCCCCTGC TGGCCTCCCT GCAGCGCATC CGCGTCCGCC TGCCCGAGGG AGACGCACTG
    CGGTATATGA TCGAGAGGAC CGTGAGCTGG CAGCACAGAG CCCGGCAGCT GCTCTCGTCA
    GGGCACCTCA AGTCCTTGCA GGACCCAGTG GGCTCAGGAC TGTTGTGTGG CAGGTGGCAG
    GCCACAGCAG GACAGGTGCC AGAGACCAGC AAGATGTCTC AGCCTCCTGG CCCCACGTCC
    TTCTCCTTGC CCGACGACTG GGACAACAGA ACCTCCTATT TACACTCTCC TTTCTCCACT
    GGTCGGAGCT GTATCCCCCT CCATGGTATC AGCCCAGAAG TGAATGAGCT GCTGATGGAG
    GCCCAGCTGC TCCAGGTGTC CCTTCCTGAA ATTCAGGAAC TCTACCAGAC GTTACTTGCA
    AAGCCGAGCC CTGCGCAGCA GGCTGACCAC AGCTCTCCAG TGAGACCCGG CAGTGAGAAG
    AGTGACGGCT GCCGAGGGAA GCGAGATGGA GCCAGCAGCC TCGAGAGCAA GCTGAAGAGA
    CGCCTGGAAA GAGAAGGCCT CTCCAGTGAG CGGTGGGATC GAATCACCAA AATGCGGGCC
    CCCAAAAAGA AGAGAATCAA ACTGAGCCAC CCCAAGGACA CGGACAGTCT CAAGTTAGAG
    AGAGAGTGTA GCTATGAGTT GGCCCCCTCT GCCGACACGC AGTCCCTGCC CTCAGACTCG
    TCCTCCTCGG AGCAGGAGGA CTCTGAGGAC GAGGACGCCA TCTGCCCGGC CGTGAGCTGC
    CTGCAGCCAG AAGGAGATGA GGTGGACTGG GTCCAGTGTG ATGGCAGCTG CAGTCGGTGG
    TTCCATCAGG TCTGTGTTGG TGTCTCTCCC GAGACGGCCG AGGAGGAGGA CTACGTGTGC
    GTGCGCTGTG CGGAGACGGA CGTGCCGGGC CGGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223795
    Clone ID Related Accession (Same CDS sequence) XM_024976577.1
    Accession Version XM_024976577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4620bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGCCC CCGGCCCGCG CCCCGTGCCG GCCCTCGGCG GTCCCGGCCC ACTGGGGGAG 
    TTCCTGCCGC CGCCCGAGTG CCCGGTCTTC GAGCCCAGCT GGGAGGAGTT CGCCGATCCC
    TTCGCCTTCA TCCACAAGAT ACGGCCCATA GCCGAGCAGA CCGGGATCTG CAAGGTGCGG
    CCGCCGCCGG ATTGGCAGCC ACCATTTGCC TGTGATGTTG ACAAACTTCA TTTTACCCCG
    CGTATCCAGA GGCTGAATGA ACTGGAGGCT CAAACACGTG TAAAATTAAA TTTCCTGGAC
    CAGATTGCAA AGTACTGGGA ATTACAGGGA AGTACTCTGA AAATTCCACA CGTGGAGAGG
    AAGATCTTGG ACTTGTTTCA GCTTAATAAG TTAGTTGCAG AGGAAGGTGG CTTTGCAGTT
    GTTTGCAAGG ATAGAAAATG GACCAAAATT GCCACCAAGA TGGGCTTTGC TCCTGGCAAA
    GCTGTGGGCT CTCATATAAG AGGACACTAC GAGCGGATTC TGAACCCCTA CAACTTATTT
    CTGTCTGGAG ACAGCTTGAG GTGTCTGCAG AAGCCAAACC TGAGCACAGA CACAAAGGAC
    AAGGAGTACA AACCCCATGG TATTCCCCAG AGGCAGTCTG TGCAGCCCGC GGAAACCTGC
    CCCCCAGCCC GACGAGCAAA ACGCATGAGA GCGGAGGCCA TGAATATTAA AATAGAACCT
    GAAGAGACAA CAGAAGCCAG AACTCATAAC CTGAGACGTC GAATGGGATG TCCAGCTCCA
    AAGTGTGAGA ATGACAAAGA CGTGAAAAGC AGCGTCAAGC AAGAACCTGT TGAAAAGAAA
    GAGTGTGTTA CAGAAAGTGA GAAGGAAAAA CCCAAGAGTC GAGCTAAAAA AACTACCAAT
    GCTGTTGACC TGTACGTCTG CCTCTTGTGT GGCAGTGGCA GCGACGAGGA CCGGCTTCTC
    CTGTGCGATG GGTGTGATGA CAGCTACCAC ACCTTCTGCC TGATCCCGCC GCTCCATGAC
    GTTCCCAAGG GAGACTGGAG GTGTCCTAAG TGTTTGGCTC AGGAATGCAG TAAGCCACAA
    GAAGCATTCG GCTTTGAGCA GGCGGCCAGG GATTATACTC TCCGTACTTT TGGTGAAATG
    GCAGATGCAT TCAAATCTGA TTACTTCAAC ATGCCAGTCC ATATGGTTCC CACGGAACTG
    GTAGAGAAGG AATTTTGGCG ACTAGTGAGC ACTATTGAAG AGGATGTCAC CGTGGAATAT
    GGAGCCGATA TTGCCTCGAA GGAGTTCGGC AGTGGCTTCC CTGTCCGAGA TGGGAAAGTC
    AAGCTCTCAC CTGAGGAGGA GGAGTATCTT GATAGTGGCT GGAATCTGAA CAACATGCCA
    GTGATGGAGC AGTCTGTTCT TGCTCACATC ACTGCCGACA TATGCGGCAT GAAGCTTCCT
    TGGCTGTACG TGGGAATGTG CTTTTCGTCC TTCTGTTGGC ACATTGAAGA CCACTGGAGC
    TATTCAATTA ACTACCTGCA CTGGGGTGAG CCAAAGACCT GGTACGGCGT TCCCGGGTAC
    GCTGCTGAGC AGCTGGAGAC CGTGATGAAG AAGCTGGCCC CAGAGCTCTT CATCTCCCAG
    CCCGACCTTC TCCACCAGCT TGTCACCATC ATGAACCCCA ACACCCTGAT GACTCACGAA
    GTGCCTGTTT ACCGAACCAA TCAGTGTGCT GGGGAGTTTG TGATCACGTT TCCAAGAGCC
    TACCACAGCG GTTTCAATCA AGGTTTCAAT TTTGCTGAGG CCGTTAACTT CTGCACTGTT
    GATTGGCTGC CCTTAGGCCG GCAGTGCGTG GAGCATTACC GTCTGCTCCA CCGCTACTGC
    GTGTTCTCCC ACGACGAGAT GATCTGCAAG ATGGCTTCCA AGGCGGATGT CCTAGACGTT
    GTAGTGGCCT CAACTGTTCA GAAAGACATG GCCATCATGA TTGAGGACGA GAAGGCACTC
    AGGGAAACTG TTCGCAAATT GGGAGTAATT GATTCGGAAA GAATGGATTT TGAACTGTTG
    CCGGATGATG AGCGTCAGTG TGTGAAATGC AAAACCACGT GCTTCATGTC TGCCCTCTCC
    TGCTGCTGCA AACCTGGCCT GCTTGTTTGC CTGCATCACG TACAGGAGTT ATGTTCCTGT
    CCTCCTTATA AATACAAACT GAGGTACAGA TACACTCTGG ATGACCTCTA CCCCATGATG
    AATGCACTGA AGCTTCGAGC AGAGTCTTAT AACGAGTGGG CCTTGAATGT GAATGAAGCT
    TTGGAGGCTA AGATTAATAA GAAGAAAAGC CTTGTCAGCT TTAAGGCTTT GATTGAAGAA
    TCTGAGATGA AGAAATTTCC AGACAACGAT CTTCTACGTC ACCTCCGCTT AGTCACACAG
    GACGCGGAGA AATGCGCCTC TGTTGCCCAG CAGCTACTTA ATGGAAAGAG GCAGACGAGA
    TACCGGTCTG GTGGAGGGAA ATCCCACAAT CAGCTGACCG TGAATGAGCT CCGGCAGTTT
    GTGACCCAGC TGCACGCCCT CCCCTGTGTC CTCAGTCAGA CACCGCTGCT GAAGGATCTC
    TTGAATCGTG TAGAAGATTT CCAACAGCAT AGCCAGAAAC TGCTCTCTGA GGAGATGCCC
    AGTGCTTCTG AGCTGCAGGA CTTGCTGGAT GTCAGCTTTG AATTTGATGT TGAGCTCCCC
    CAGCTTGCTG AGATGCGTGT CCGGCTGGAG CAGGCCCATT GGCTGGAGGA GGTGCAGCAG
    GCTTGCCTGG ACCCGAGCTC CCTCACCTTA GATGATATGA GACGTCTCAT AGACCTGGGA
    GTGGGCCTGG CCCCATATTC AGCCGTGGAG AAGGCTATGG CGCGGCTTCA GGAACTGCTC
    ACAGTATCAG AGCACTGGGA TGACAAAGCC AAGAGTCTCC TCAAGGCCAG GCCTCGGCAT
    TCACTGAACA GCCTCGCCGC CGTGGTGAAG GAGATTGAGG AGATCCCCGC ACACCTGCCC
    AACGGCGCGG CGCTGAAAGA CTCTGTGCAG AGAGCCAGAG ACTGGCTTCA GGAAGTGGAG
    GCCCTGCAGG CTGGAGGACG TGTGCCAGTG TTGGACACGC TGGTGGAGCT TGTCACTCGA
    GGCCGGTGTA TCCCGGTGCA TCTACCCTCG CTGCCGAGAC TGGAGTCCCT AGTAGCTGAA
    GTTCACGTTT GGAAAGAATG TGCTGCTAAT ACATTCCTGA CTAAGAACTC GTCCTATTCT
    CTCCTAGAGG TGCTGTGTCC TCGATGTGAT ATTGGCCTTT TGGGATTAAA GAGGAAGCAG
    AGAAAGTTAA AGGATCCCTT GCCAAGTGGC AAGAAGAAGA GTCCCCGTGT AGAGAGTCTG
    AGCGACCTGG AGAGAACGCT GATGGAAAGC AGAGAGACTG CTTCAGCTAT GGCAGCACTC
    GGGGAAGCTC GCGCACGAGA AATGGAAGCC TTGCAGTCTC TCCGACAAGC CAATGAGGAG
    AAACTGCTGT CGCCTGTGCA CGATGTGGAG ATGAGAGTCT GCGTGTGTCA GAAGGCTCCC
    GCGGCCCCCA TGGTCCAGTG TGAACTCTGC AGGGATGCTT TCCACACCAG CTGTGTGGCA
    GCTCCCAGCA TTCCACAGGG CCCCCGAGTC TGGCTGTGTC CCAACTGTCG GAGGTCGGAG
    AAGCCTCCAC TCGAGAAGAT TCTGCCCCTG CTGGCCTCCC TGCAGCGCAT CCGCGTCCGC
    CTGCCCGAGG GAGACGCACT GCGGTATATG ATCGAGAGGA CCGTGAGCTG GCAGCACAGA
    GCCCGGCAGC TGCTCTCGTC AGGGCACCTC AAGTCCTTGC AGGACCCAGT GGGCTCAGGA
    CTGTTGTGTG GCAGGTGGCA GGCCACAGCA GGACAGGTGC CAGAGACCAG CAAGATATCT
    CAGCCTCCTG GCCCCACGTC CTTCTCCTTG CCCGACGACT GGGACAACAG AACCTCCTAT
    TTACACTCTC CTTTCTCCAC TGGTCGGAGC TGTATCCCCC TCCATGGTAT CAGCCCAGAA
    GTGAATGAGC TGCTGATGGA GGCCCAGCTG CTCCAGGTGT CCCTTCCTGA AATTCAGGAA
    CTCTACCAGA CGTTACTTGC AAAGCCGAGC CCTGCGCAGC AGGCTGACCA CAGCTCTCCA
    GTGAGACCCG GCAGTGAGAA GAGTGACGGC TGCCGAGGGA AGCGAGATGG AGCCAGCAGC
    CTCGAGAGCA AGCTGAAGAG ACGCCTGGAA AGAGAAGGCC TCTCCAGTGA GCGGTGGGAT
    CGAATCACCA AAATGCGGGC CCCCAAAAAG AAGAGAATCA AACTGAGCCA CCCCAAGGAC
    ACGGACAGTC TCAAGTTAGA GAGAGAGTGT AGCTATGAGT TGGCCCCCTC TGCCGACACG
    CAGTCCCTGC CCTCAGACTC GTCCTCCTCG GAGCAGGAGG ACTCTGAGGA CGAGGACGCC
    ATCTGCCCGG CCGTGAGCTG CCTGCAGCCA GAAGGAGATG AGGTGGACTG GGTCCAGTGT
    GATGGCAGCT GCAGTCGGTG GTTCCATCAG GTCTGTGTTG GTGTCTCTCC CGAGACGGCC
    GAGGAGGAGG ACTACGTGTG CGTGCGCTGT GCGGAGACGG ACGTGCCGGG CCGGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832345.1
    CDS271..4890
    Translation

    Target ORF information:

    RefSeq Version XM_024976577.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 5B (KDM5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGCCC CCGGCCCGCG CCCCGTGCCG GCCCTCGGCG GTCCCGGCCC ACTGGGGGAG 
    TTCCTGCCGC CGCCCGAGTG CCCGGTCTTC GAGCCCAGCT GGGAGGAGTT CGCCGATCCC
    TTCGCCTTCA TCCACAAGAT ACGGCCCATA GCCGAGCAGA CCGGGATCTG CAAGGTGCGG
    CCGCCGCCGG ATTGGCAGCC ACCATTTGCC TGTGATGTTG ACAAACTTCA TTTTACCCCG
    CGTATCCAGA GGCTGAATGA ACTGGAGGCT CAAACACGTG TAAAATTAAA TTTCCTGGAC
    CAGATTGCAA AGTACTGGGA ATTACAGGGA AGTACTCTGA AAATTCCACA CGTGGAGAGG
    AAGATCTTGG ACTTGTTTCA GCTTAATAAG TTAGTTGCAG AGGAAGGTGG CTTTGCAGTT
    GTTTGCAAGG ATAGAAAATG GACCAAAATT GCCACCAAGA TGGGCTTTGC TCCTGGCAAA
    GCTGTGGGCT CTCATATAAG AGGACACTAC GAGCGGATTC TGAACCCCTA CAACTTATTT
    CTGTCTGGAG ACAGCTTGAG GTGTCTGCAG AAGCCAAACC TGAGCACAGA CACAAAGGAC
    AAGGAGTACA AACCCCATGG TATTCCCCAG AGGCAGTCTG TGCAGCCCGC GGAAACCTGC
    CCCCCAGCCC GACGAGCAAA ACGCATGAGA GCGGAGGCCA TGAATATTAA AATAGAACCT
    GAAGAGACAA CAGAAGCCAG AACTCATAAC CTGAGACGTC GAATGGGATG TCCAGCTCCA
    AAGTGTGAGA ATGACAAAGA CGTGAAAAGC AGCGTCAAGC AAGAACCTGT TGAAAAGAAA
    GAGTGTGTTA CAGAAAGTGA GAAGGAAAAA CCCAAGAGTC GAGCTAAAAA AACTACCAAT
    GCTGTTGACC TGTACGTCTG CCTCTTGTGT GGCAGTGGCA GCGACGAGGA CCGGCTTCTC
    CTGTGCGATG GGTGTGATGA CAGCTACCAC ACCTTCTGCC TGATCCCGCC GCTCCATGAC
    GTTCCCAAGG GAGACTGGAG GTGTCCTAAG TGTTTGGCTC AGGAATGCAG TAAGCCACAA
    GAAGCATTCG GCTTTGAGCA GGCGGCCAGG GATTATACTC TCCGTACTTT TGGTGAAATG
    GCAGATGCAT TCAAATCTGA TTACTTCAAC ATGCCAGTCC ATATGGTTCC CACGGAACTG
    GTAGAGAAGG AATTTTGGCG ACTAGTGAGC ACTATTGAAG AGGATGTCAC CGTGGAATAT
    GGAGCCGATA TTGCCTCGAA GGAGTTCGGC AGTGGCTTCC CTGTCCGAGA TGGGAAAGTC
    AAGCTCTCAC CTGAGGAGGA GGAGTATCTT GATAGTGGCT GGAATCTGAA CAACATGCCA
    GTGATGGAGC AGTCTGTTCT TGCTCACATC ACTGCCGACA TATGCGGCAT GAAGCTTCCT
    TGGCTGTACG TGGGAATGTG CTTTTCGTCC TTCTGTTGGC ACATTGAAGA CCACTGGAGC
    TATTCAATTA ACTACCTGCA CTGGGGTGAG CCAAAGACCT GGTACGGCGT TCCCGGGTAC
    GCTGCTGAGC AGCTGGAGAC CGTGATGAAG AAGCTGGCCC CAGAGCTCTT CATCTCCCAG
    CCCGACCTTC TCCACCAGCT TGTCACCATC ATGAACCCCA ACACCCTGAT GACTCACGAA
    GTGCCTGTTT ACCGAACCAA TCAGTGTGCT GGGGAGTTTG TGATCACGTT TCCAAGAGCC
    TACCACAGCG GTTTCAATCA AGGTTTCAAT TTTGCTGAGG CCGTTAACTT CTGCACTGTT
    GATTGGCTGC CCTTAGGCCG GCAGTGCGTG GAGCATTACC GTCTGCTCCA CCGCTACTGC
    GTGTTCTCCC ACGACGAGAT GATCTGCAAG ATGGCTTCCA AGGCGGATGT CCTAGACGTT
    GTAGTGGCCT CAACTGTTCA GAAAGACATG GCCATCATGA TTGAGGACGA GAAGGCACTC
    AGGGAAACTG TTCGCAAATT GGGAGTAATT GATTCGGAAA GAATGGATTT TGAACTGTTG
    CCGGATGATG AGCGTCAGTG TGTGAAATGC AAAACCACGT GCTTCATGTC TGCCCTCTCC
    TGCTGCTGCA AACCTGGCCT GCTTGTTTGC CTGCATCACG TACAGGAGTT ATGTTCCTGT
    CCTCCTTATA AATACAAACT GAGGTACAGA TACACTCTGG ATGACCTCTA CCCCATGATG
    AATGCACTGA AGCTTCGAGC AGAGTCTTAT AACGAGTGGG CCTTGAATGT GAATGAAGCT
    TTGGAGGCTA AGATTAATAA GAAGAAAAGC CTTGTCAGCT TTAAGGCTTT GATTGAAGAA
    TCTGAGATGA AGAAATTTCC AGACAACGAT CTTCTACGTC ACCTCCGCTT AGTCACACAG
    GACGCGGAGA AATGCGCCTC TGTTGCCCAG CAGCTACTTA ATGGAAAGAG GCAGACGAGA
    TACCGGTCTG GTGGAGGGAA ATCCCACAAT CAGCTGACCG TGAATGAGCT CCGGCAGTTT
    GTGACCCAGC TGCACGCCCT CCCCTGTGTC CTCAGTCAGA CACCGCTGCT GAAGGATCTC
    TTGAATCGTG TAGAAGATTT CCAACAGCAT AGCCAGAAAC TGCTCTCTGA GGAGATGCCC
    AGTGCTTCTG AGCTGCAGGA CTTGCTGGAT GTCAGCTTTG AATTTGATGT TGAGCTCCCC
    CAGCTTGCTG AGATGCGTGT CCGGCTGGAG CAGGCCCATT GGCTGGAGGA GGTGCAGCAG
    GCTTGCCTGG ACCCGAGCTC CCTCACCTTA GATGATATGA GACGTCTCAT AGACCTGGGA
    GTGGGCCTGG CCCCATATTC AGCCGTGGAG AAGGCTATGG CGCGGCTTCA GGAACTGCTC
    ACAGTATCAG AGCACTGGGA TGACAAAGCC AAGAGTCTCC TCAAGGCCAG GCCTCGGCAT
    TCACTGAACA GCCTCGCCGC CGTGGTGAAG GAGATTGAGG AGATCCCCGC ACACCTGCCC
    AACGGCGCGG CGCTGAAAGA CTCTGTGCAG AGAGCCAGAG ACTGGCTTCA GGAAGTGGAG
    GCCCTGCAGG CTGGAGGACG TGTGCCAGTG TTGGACACGC TGGTGGAGCT TGTCACTCGA
    GGCCGGTGTA TCCCGGTGCA TCTACCCTCG CTGCCGAGAC TGGAGTCCCT AGTAGCTGAA
    GTTCACGTTT GGAAAGAATG TGCTGCTAAT ACATTCCTGA CTAAGAACTC GTCCTATTCT
    CTCCTAGAGG TGCTGTGTCC TCGATGTGAT ATTGGCCTTT TGGGATTAAA GAGGAAGCAG
    AGAAAGTTAA AGGATCCCTT GCCAAGTGGC AAGAAGAAGA GTCCCCGTGT AGAGAGTCTG
    AGCGACCTGG AGAGAACGCT GATGGAAAGC AGAGAGACTG CTTCAGCTAT GGCAGCACTC
    GGGGAAGCTC GCGCACGAGA AATGGAAGCC TTGCAGTCTC TCCGACAAGC CAATGAGGAG
    AAACTGCTGT CGCCTGTGCA CGATGTGGAG ATGAGAGTCT GCGTGTGTCA GAAGGCTCCC
    GCGGCCCCCA TGGTCCAGTG TGAACTCTGC AGGGATGCTT TCCACACCAG CTGTGTGGCA
    GCTCCCAGCA TTCCACAGGG CCCCCGAGTC TGGCTGTGTC CCAACTGTCG GAGGTCGGAG
    AAGCCTCCAC TCGAGAAGAT TCTGCCCCTG CTGGCCTCCC TGCAGCGCAT CCGCGTCCGC
    CTGCCCGAGG GAGACGCACT GCGGTATATG ATCGAGAGGA CCGTGAGCTG GCAGCACAGA
    GCCCGGCAGC TGCTCTCGTC AGGGCACCTC AAGTCCTTGC AGGACCCAGT GGGCTCAGGA
    CTGTTGTGTG GCAGGTGGCA GGCCACAGCA GGACAGGTGC CAGAGACCAG CAAGATATCT
    CAGCCTCCTG GCCCCACGTC CTTCTCCTTG CCCGACGACT GGGACAACAG AACCTCCTAT
    TTACACTCTC CTTTCTCCAC TGGTCGGAGC TGTATCCCCC TCCATGGTAT CAGCCCAGAA
    GTGAATGAGC TGCTGATGGA GGCCCAGCTG CTCCAGGTGT CCCTTCCTGA AATTCAGGAA
    CTCTACCAGA CGTTACTTGC AAAGCCGAGC CCTGCGCAGC AGGCTGACCA CAGCTCTCCA
    GTGAGACCCG GCAGTGAGAA GAGTGACGGC TGCCGAGGGA AGCGAGATGG AGCCAGCAGC
    CTCGAGAGCA AGCTGAAGAG ACGCCTGGAA AGAGAAGGCC TCTCCAGTGA GCGGTGGGAT
    CGAATCACCA AAATGCGGGC CCCCAAAAAG AAGAGAATCA AACTGAGCCA CCCCAAGGAC
    ACGGACAGTC TCAAGTTAGA GAGAGAGTGT AGCTATGAGT TGGCCCCCTC TGCCGACACG
    CAGTCCCTGC CCTCAGACTC GTCCTCCTCG GAGCAGGAGG ACTCTGAGGA CGAGGACGCC
    ATCTGCCCGG CCGTGAGCTG CCTGCAGCCA GAAGGAGATG AGGTGGACTG GGTCCAGTGT
    GATGGCAGCT GCAGTCGGTG GTTCCATCAG GTCTGTGTTG GTGTCTCTCC CGAGACGGCC
    GAGGAGGAGG ACTACGTGTG CGTGCGCTGT GCGGAGACGG ACGTGCCGGG CCGGAGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.