PRKAA1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKAA1
Entrez Gene ID	540404
Full Name	protein kinase AMP-activated catalytic subunit alpha 1
General protein information	Preferred Names protein kinase AMP-activated catalytic subunit alpha 1 Names 5'-AMP-activated protein kinase catalytic subunit alpha-1 protein kinase, AMP-activated, alpha 1 catalytic subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024981361.1	XP_024837129.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1
XM_024981363.1	XP_024837131.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X3
NM_001109802.2	NP_001103272.1	5'-AMP-activated protein kinase catalytic subunit alpha-1
XM_005221557.2	XP_005221614.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1
XM_005221557.3	XP_005221614.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X2
XM_024981362.1	XP_024837130.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X3
XM_010816788.2	XP_010815090.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PRKAA1	NP_001034692.1
Arabidopsis thaliana (thale cress)	KIN11	NP_566843.1
Oryza sativa (rice)	Os05g0530500	NP_001056128.1
Pan troglodytes (chimpanzee)	PRKAA1	XP_003310787.1
Bos taurus (cattle)	PRKAA1	NP_001103272.1
Arabidopsis thaliana (thale cress)	KIN10	NP_566130.1
Danio rerio (zebrafish)	prkaa1	NP_001103756.1
Canis lupus familiaris (dog)	PRKAA1	XP_536491.4
Mus musculus (house mouse)	Prkaa1	NP_001013385.3
Homo sapiens (human)	PRKAA1	NP_996790.3
	PRKAA1	XP_001086285.1
Rattus norvegicus (Norway rat)	Prkaa1	NP_062015.2
Xenopus tropicalis (tropical clawed frog)	prkaa1	NP_001120434.1

Gene Ontology
Bibliography

Related articles in PubMed

Glucose Availability and AMP-Activated Protein Kinase Link Energy Metabolism and Innate Immunity in the Bovine Endometrium.
Turner ML, Cronin JG, Noleto PG, Sheldon IM
PloS one11(3)e0151416(2016)

Energy depletion of bovine mammary epithelial cells activates AMPK and suppresses protein synthesis through inhibition of mTORC1 signaling.
Burgos SA, Kim JJ, Dai M, Cant JP
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(3)183-9(2013 Mar)

Activation of the AMP-activated protein kinase (AMPK) by nitrated lipids in endothelial cells.
Wu Y, Dong Y, Song P, Zou MH
PloS one7(2)e31056(2012)

Ischemia-induced stimulation of Na-K-Cl cotransport in cerebral microvascular endothelial cells involves AMP kinase.
Wallace BK, Foroutan S, O'Donnell ME
American journal of physiology. Cell physiology301(2)C316-26(2011 Aug)

Redox regulation of the AMP-activated protein kinase.
Han Y, Wang Q, Song P, Zhu Y, Zou MH
PloS one5(11)e15420(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endometrial inflammatory responses to lipopolysaccharide were also reduced by small molecules that activate or inhibit the intracellular sensor of energy, AMP-activated protein kinase (AMPK).
Title: Glucose Availability and AMP-Activated Protein Kinase Link Energy Metabolism and Innate Immunity in the Bovine Endometrium.

The results suggest that activation of AMPK represses global protein synthesis in mammary epithelial cells through inhibition of mTORC1 signaling.
Title: Energy depletion of bovine mammary epithelial cells activates AMPK and suppresses protein synthesis through inhibition of mTORC1 signaling.

Nitrated oleic acid activates AMPK in endothelial cells.
Title: Activation of the AMP-activated protein kinase (AMPK) by nitrated lipids in endothelial cells.

Findings suggest that ischemic factor stimulation of the blood brain barrier Na-K-Cl cotransporter involves activation of AMPK.
Title: Ischemia-induced stimulation of Na-K-Cl cotransport in cerebral microvascular endothelial cells involves AMP kinase.

Results suggest that mitochondria-derived superoxide anions and peroxynitrite are required for Berberine-induced AMPK activation in endothelial cells.
Title: Redox regulation of the AMP-activated protein kinase.

The following PRKAA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKAA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa40068	NM_001109802.2 Latest version!	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa30647	XM_005221557.3 Latest version!	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa40068	XM_010816788.2 Latest version!	Bos taurus protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa228063	XM_024981362.1 Latest version!	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa228063	XM_024981363.1 Latest version!	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa228062	XM_024981361.1 Latest version!	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OBa30647	XM_005221557.2	Bos taurus protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKAA1 ( NM_001109802.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001103272.1 Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), mRNA.

Price & Availability↑

CloneID	OBa40068
Clone ID Related Accession (Same CDS sequence)	NM_001109802.2 , XM_010816788.2
Accession Version	NM_001109802.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC153842.1. On Aug 30, 2012 this sequence version replaced NM_001109802.1. ##Evidence-Data-START## Transcript exon combination :: BC153842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGTGATGG AATATGTCTC AGGAGGAGAG CTATTTGATT ATATCTGTAA AAATGGAAGG 
CTGGATGAAA AAGAAAGTCG ACGACTGTTC CAACAAATCC TTTCTGGTGT GGATTACTGT 
CACAGGCATA TGGTGGTCCA CAGAGATTTG AAACCTGAAA ACGTCCTGCT TGATGCACAC 
ATGAATGCAA AGATAGCTGA TTTTGGTCTT TCAAACATGA TGTCAGATGG TGAATTTTTA 
AGAACAAGTT GTGGCTCACC CAACTATGCT GCACCAGAAG TAATTTCAGG AAGATTGTAT 
GCTGGTCCAG AGGTAGATAT ATGGAGCAGT GGGGTTATTC TGTATGCTTT ATTATGTGGA 
ACCCTTCCGT TTGATGATGA TCACGTGCCA ACTCTTTTTA AGAAGATATG TGATGGGATC 
TTTTATACCC CTCAATATTT AAATCCTTCT GTGATTAGCC TTTTGAAACA TATGCTGCAG 
GTGGATCCCA TGAAGAGAGC CACAATCAAA GATATCAGGG AACATGAATG GTTTAAACAG 
GACCTTCCAA AATATCTCTT TCCTGAGGAT CCATCATATA GCTCAACCAT GATTGATGAT 
GAAGCCTTAA AAGAAGTATG TGAAAAATTT GAATGCTCAG AGGAGGAGGT TCTCAGCTGC 
CTTTATAACA GAAATCACCA GGACCCTTTG GCAGTTGCCT ACCACCTCAT AATAGATAAC 
AGGAGGATAA TGAACGAGGC CAAAGATTTT TACTTGGCAA CGAGCCCACC AGATTCGTTT 
CTCGACGATC ACCACTTGAC TCGGCCCCAT CCTGAAAGAG TACCATTCTT GGTTGCTGAA 
ACTCCCAGGG CGCGCCATAC CCTTGATGAA TTAAATCCAC AGAAATCCAA ACACCAAGGT 
GTAAGGAAAG CAAAATGGCA TTTGGGAATT AGAAGTCAAA GTCGGCCAAA TGATATCATG 
GCAGAAGTTT GTAGAGCAAT TAAACAGCTG GATTATGAAT GGAAGGTTGT AAACCCGTAT 
TATTTGCGTG TTCGGAGGAA GAATCCTGTG ACAAGTACAT ACTCCAAAAT GAGTCTGCAG 
TTATACCAAG TGGATAGTAG AACGTATTTA CTGGATTTCC GAAGTATTGA TGATGAAATT 
ACTGAAGCCA AATCAGGGAC TGCTACGCCA CAGAGATCGG GCTCAGTTAG CAACTATCGA 
GCTTGCCAAA GGAATGATTC AGATGCTGAG GCTCAAGGAA AATCCTCAGA AGCTTCTCTT 
ACCTCATCTG TAACCTCACT TGACTCTTCT CCTGTTGAAT TAACTCCAAG ACCTGGAAGT 
CACACAATAG AATTTTTTGA GATGTGCGCG AATCTAATTA AAATTCTTGC ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103272.1
CDS	424..1800
Translation	MVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRACQRNDSDAEAQGKSSEASLTSSVTSLDSSPVELTPRPGSHTIEFFEMCANLIKILAQ

Target ORF information:

RefSeq Version	NM_001109802.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109802.2

ORF Insert Sequence:

ATGGTGATGG AATATGTCTC AGGAGGAGAG CTATTTGATT ATATCTGTAA AAATGGAAGG 
CTGGATGAAA AAGAAAGTCG ACGACTGTTC CAACAAATCC TTTCTGGTGT GGATTACTGT 
CACAGGCATA TGGTGGTCCA CAGAGATTTG AAACCTGAAA ACGTCCTGCT TGATGCACAC 
ATGAATGCAA AGATAGCTGA TTTTGGTCTT TCAAACATGA TGTCAGATGG TGAATTTTTA 
AGAACAAGTT GTGGCTCACC CAACTATGCT GCACCAGAAG TAATTTCAGG AAGATTGTAT 
GCTGGTCCAG AGGTAGATAT ATGGAGCAGT GGGGTTATTC TGTATGCTTT ATTATGTGGA 
ACCCTTCCGT TTGATGATGA TCACGTGCCA ACTCTTTTTA AGAAGATATG TGATGGGATC 
TTTTATACCC CTCAATATTT AAATCCTTCT GTGATTAGCC TTTTGAAACA TATGCTGCAG 
GTGGATCCCA TGAAGAGAGC CACAATCAAA GATATCAGGG AACATGAATG GTTTAAACAG 
GACCTTCCAA AATATCTCTT TCCTGAGGAT CCATCATATA GCTCAACCAT GATTGATGAT 
GAAGCCTTAA AAGAAGTATG TGAAAAATTT GAATGCTCAG AGGAGGAGGT TCTCAGCTGC 
CTTTATAACA GAAATCACCA GGACCCTTTG GCAGTTGCCT ACCACCTCAT AATAGATAAC 
AGGAGGATAA TGAACGAGGC CAAAGATTTT TACTTGGCAA CGAGCCCACC AGATTCGTTT 
CTCGACGATC ACCACTTGAC TCGGCCCCAT CCTGAAAGAG TACCATTCTT GGTTGCTGAA 
ACTCCCAGGG CGCGCCATAC CCTTGATGAA TTAAATCCAC AGAAATCCAA ACACCAAGGT 
GTAAGGAAAG CAAAATGGCA TTTGGGAATT AGAAGTCAAA GTCGGCCAAA TGATATCATG 
GCAGAAGTTT GTAGAGCAAT TAAACAGCTG GATTATGAAT GGAAGGTTGT AAACCCGTAT 
TATTTGCGTG TTCGGAGGAA GAATCCTGTG ACAAGTACAT ACTCCAAAAT GAGTCTGCAG 
TTATACCAAG TGGATAGTAG AACGTATTTA CTGGATTTCC GAAGTATTGA TGATGAAATT 
ACTGAAGCCA AATCAGGGAC TGCTACGCCA CAGAGATCGG GCTCAGTTAG CAACTATCGA 
GCTTGCCAAA GGAATGATTC AGATGCTGAG GCTCAAGGAA AATCCTCAGA AGCTTCTCTT 
ACCTCATCTG TAACCTCACT TGACTCTTCT CCTGTTGAAT TAACTCCAAG ACCTGGAAGT 
CACACAATAG AATTTTTTGA GATGTGCGCG AATCTAATTA AAATTCTTGC ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA1 ( XM_005221557.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221614.1 Bos taurus protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30647
Clone ID Related Accession (Same CDS sequence)	XM_005221557.2 , XM_005221557.3
Accession Version	XM_005221557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104514.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005221557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGAC TCAGTTCCTG GAGAAAGATG GCGACAGCCG AGAAGCAGAA ACACGACGGG 
CGGGTGAAGA TCGGTCACTA CATCCTGGGG GATACGCTGG GGGTCGGGAC CTTCGGCAAA 
GTGAAGGTTG GCAAACATGA ATTGACTGGG CATAAAGTTG CTGTGAAGAT ACTGAATCGG 
CAGAAGATTC GAAGCCTTGA TGTAGTAGGA AAAATCCGCA GAGAAATTCA GAACCTCAAG 
CTTTTCAGGC ATCCTCATAT AATTAAACTG TACCAGGTCA TCAGTACACC ATCTGATATT 
TTCATGGTGA TGGAATATGT CTCAGGAGGA GAGCTATTTG ATTATATCTG TAAAAATGGA 
AGGCTGGATG AAAAAGAAAG TCGACGACTG TTCCAACAAA TCCTTTCTGG TGTGGATTAC 
TGTCACAGGC ATATGGTGGT CCACAGAGAT TTGAAACCTG AAAACGTCCT GCTTGATGCA 
CACATGAATG CAAAGATAGC TGATTTTGGT CTTTCAAACA TGATGTCAGA TGGTGAATTT 
TTAAGAACAA GTTGTGGCTC ACCCAACTAT GCTGCACCAG AAGTAATTTC AGGAAGATTG 
TATGCTGGTC CAGAGGTAGA TATATGGAGC AGTGGGGTTA TTCTGTATGC TTTATTATGT 
GGAACCCTTC CGTTTGATGA TGATCACGTG CCAACTCTTT TTAAGAAGAT ATGTGATGGG 
ATCTTTTATA CCCCTCAATA TTTAAATCCT TCTGTGATTA GCCTTTTGAA ACATATGCTG 
CAGGTGGATC CCATGAAGAG AGCCACAATC AAAGATATCA GGGAACATGA ATGGTTTAAA 
CAGGACCTTC CAAAATATCT CTTTCCTGAG GATCCATCAT ATAGCTCAAC CATGATTGAT 
GATGAAGCCT TAAAAGAAGT ATGTGAAAAA TTTGAATGCT CAGAGGAGGA GGTTCTCAGC 
TGCCTTTATA ACAGAAATCA CCAGGACCCT TTGGCAGTTG CCTACCACCT CATAATAGAT 
AACAGGAGGA TAATGAACGA GGCCAAAGAT TTTTACTTGG CAACGAGCCC ACCAGATTCG 
TTTCTCGACG ATCACCACTT GACTCGGCCC CATCCTGAAA GAGTACCATT CTTGGTTGCT 
GAAACTCCCA GGGCGCGCCA TACCCTTGAT GAATTAAATC CACAGAAATC CAAACACCAA 
GGTGTAAGGA AAGCAAAATG GCATTTGGGA ATTAGAAGTC AAAGTCGGCC AAATGATATC 
ATGGCAGAAG TTTGTAGAGC AATTAAACAG CTGGATTATG AATGGAAGGT TGTAAACCCG 
TATTATTTGC GTGTTCGGAG GAAGAATCCT GTGACAAGTA CATACTCCAA AATGAGTCTG 
CAGTTATACC AAGTGGATAG TAGAACGTAT TTACTGGATT TCCGAAGTAT TGATGATGAA 
ATTACTGAAG CCAAATCAGG GACTGCTACG CCACAGAGAT CGGGCTCAGT TAGCAACTAT 
CGAGCTTGCC AAAGGAATGA TTCAGATGCT GAGGCTCAAG GAAAATCCTC AGAAGCTTCT 
CTTACCTCAT CTGTAACCTC ACTTGACTCT TCTCCTGTTG AATTAACTCC AAGACCTGGA 
AGTCACACAA TAGAATTTTT TGAGATGTGC GCGAATCTAA TTAAAATTCT TGCACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221614.1
CDS	18..1697
Translation	MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRACQRNDSDAEAQGKSSEASLTSSVTSLDSSPVELTPRPGSHTIEFFEMCANLIKILAQ

Target ORF information:

RefSeq Version	XM_005221557.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221557.2

ORF Insert Sequence:

ATGCGCAGAC TCAGTTCCTG GAGAAAGATG GCGACAGCCG AGAAGCAGAA ACACGACGGG 
CGGGTGAAGA TCGGTCACTA CATCCTGGGG GATACGCTGG GGGTCGGGAC CTTCGGCAAA 
GTGAAGGTTG GCAAACATGA ATTGACTGGG CATAAAGTTG CTGTGAAGAT ACTGAATCGG 
CAGAAGATTC GAAGCCTTGA TGTAGTAGGA AAAATCCGCA GAGAAATTCA GAACCTCAAG 
CTTTTCAGGC ATCCTCATAT AATTAAACTG TACCAGGTCA TCAGTACACC ATCTGATATT 
TTCATGGTGA TGGAATATGT CTCAGGAGGA GAGCTATTTG ATTATATCTG TAAAAATGGA 
AGGCTGGATG AAAAAGAAAG TCGACGACTG TTCCAACAAA TCCTTTCTGG TGTGGATTAC 
TGTCACAGGC ATATGGTGGT CCACAGAGAT TTGAAACCTG AAAACGTCCT GCTTGATGCA 
CACATGAATG CAAAGATAGC TGATTTTGGT CTTTCAAACA TGATGTCAGA TGGTGAATTT 
TTAAGAACAA GTTGTGGCTC ACCCAACTAT GCTGCACCAG AAGTAATTTC AGGAAGATTG 
TATGCTGGTC CAGAGGTAGA TATATGGAGC AGTGGGGTTA TTCTGTATGC TTTATTATGT 
GGAACCCTTC CGTTTGATGA TGATCACGTG CCAACTCTTT TTAAGAAGAT ATGTGATGGG 
ATCTTTTATA CCCCTCAATA TTTAAATCCT TCTGTGATTA GCCTTTTGAA ACATATGCTG 
CAGGTGGATC CCATGAAGAG AGCCACAATC AAAGATATCA GGGAACATGA ATGGTTTAAA 
CAGGACCTTC CAAAATATCT CTTTCCTGAG GATCCATCAT ATAGCTCAAC CATGATTGAT 
GATGAAGCCT TAAAAGAAGT ATGTGAAAAA TTTGAATGCT CAGAGGAGGA GGTTCTCAGC 
TGCCTTTATA ACAGAAATCA CCAGGACCCT TTGGCAGTTG CCTACCACCT CATAATAGAT 
AACAGGAGGA TAATGAACGA GGCCAAAGAT TTTTACTTGG CAACGAGCCC ACCAGATTCG 
TTTCTCGACG ATCACCACTT GACTCGGCCC CATCCTGAAA GAGTACCATT CTTGGTTGCT 
GAAACTCCCA GGGCGCGCCA TACCCTTGAT GAATTAAATC CACAGAAATC CAAACACCAA 
GGTGTAAGGA AAGCAAAATG GCATTTGGGA ATTAGAAGTC AAAGTCGGCC AAATGATATC 
ATGGCAGAAG TTTGTAGAGC AATTAAACAG CTGGATTATG AATGGAAGGT TGTAAACCCG 
TATTATTTGC GTGTTCGGAG GAAGAATCCT GTGACAAGTA CATACTCCAA AATGAGTCTG 
CAGTTATACC AAGTGGATAG TAGAACGTAT TTACTGGATT TCCGAAGTAT TGATGATGAA 
ATTACTGAAG CCAAATCAGG GACTGCTACG CCACAGAGAT CGGGCTCAGT TAGCAACTAT 
CGAGCTTGCC AAAGGAATGA TTCAGATGCT GAGGCTCAAG GAAAATCCTC AGAAGCTTCT 
CTTACCTCAT CTGTAACCTC ACTTGACTCT TCTCCTGTTG AATTAACTCC AAGACCTGGA 
AGTCACACAA TAGAATTTTT TGAGATGTGC GCGAATCTAA TTAAAATTCT TGCACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA1 ( XM_005221557.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221614.1 Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30647
Clone ID Related Accession (Same CDS sequence)	XM_005221557.2 , XM_005221557.3
Accession Version	XM_005221557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGAC TCAGTTCCTG GAGAAAGATG GCGACAGCCG AGAAGCAGAA ACACGACGGG 
CGGGTGAAGA TCGGTCACTA CATCCTGGGG GATACGCTGG GGGTCGGGAC CTTCGGCAAA 
GTGAAGGTTG GCAAACATGA ATTGACTGGG CATAAAGTTG CTGTGAAGAT ACTGAATCGG 
CAGAAGATTC GAAGCCTTGA TGTAGTAGGA AAAATCCGCA GAGAAATTCA GAACCTCAAG 
CTTTTCAGGC ATCCTCATAT AATTAAACTG TACCAGGTCA TCAGTACACC ATCTGATATT 
TTCATGGTGA TGGAATATGT CTCAGGAGGA GAGCTATTTG ATTATATCTG TAAAAATGGA 
AGGCTGGATG AAAAAGAAAG TCGACGACTG TTCCAACAAA TCCTTTCTGG TGTGGATTAC 
TGTCACAGGC ATATGGTGGT CCACAGAGAT TTGAAACCTG AAAACGTCCT GCTTGATGCA 
CACATGAATG CAAAGATAGC TGATTTTGGT CTTTCAAACA TGATGTCAGA TGGTGAATTT 
TTAAGAACAA GTTGTGGCTC ACCCAACTAT GCTGCACCAG AAGTAATTTC AGGAAGATTG 
TATGCTGGTC CAGAGGTAGA TATATGGAGC AGTGGGGTTA TTCTGTATGC TTTATTATGT 
GGAACCCTTC CGTTTGATGA TGATCACGTG CCAACTCTTT TTAAGAAGAT ATGTGATGGG 
ATCTTTTATA CCCCTCAATA TTTAAATCCT TCTGTGATTA GCCTTTTGAA ACATATGCTG 
CAGGTGGATC CCATGAAGAG AGCCACAATC AAAGATATCA GGGAACATGA ATGGTTTAAA 
CAGGACCTTC CAAAATATCT CTTTCCTGAG GATCCATCAT ATAGCTCAAC CATGATTGAT 
GATGAAGCCT TAAAAGAAGT ATGTGAAAAA TTTGAATGCT CAGAGGAGGA GGTTCTCAGC 
TGCCTTTATA ACAGAAATCA CCAGGACCCT TTGGCAGTTG CCTACCACCT CATAATAGAT 
AACAGGAGGA TAATGAACGA GGCCAAAGAT TTTTACTTGG CAACGAGCCC ACCAGATTCG 
TTTCTCGACG ATCACCACTT GACTCGGCCC CATCCTGAAA GAGTACCATT CTTGGTTGCT 
GAAACTCCCA GGGCGCGCCA TACCCTTGAT GAATTAAATC CACAGAAATC CAAACACCAA 
GGTGTAAGGA AAGCAAAATG GCATTTGGGA ATTAGAAGTC AAAGTCGGCC AAATGATATC 
ATGGCAGAAG TTTGTAGAGC AATTAAACAG CTGGATTATG AATGGAAGGT TGTAAACCCG 
TATTATTTGC GTGTTCGGAG GAAGAATCCT GTGACAAGTA CATACTCCAA AATGAGTCTG 
CAGTTATACC AAGTGGATAG TAGAACGTAT TTACTGGATT TCCGAAGTAT TGATGATGAA 
ATTACTGAAG CCAAATCAGG GACTGCTACG CCACAGAGAT CGGGCTCAGT TAGCAACTAT 
CGAGCTTGCC AAAGGAATGA TTCAGATGCT GAGGCTCAAG GAAAATCCTC AGAAGCTTCT 
CTTACCTCAT CTGTAACCTC ACTTGACTCT TCTCCTGTTG AATTAACTCC AAGACCTGGA 
AGTCACACAA TAGAATTTTT TGAGATGTGC GCGAATCTAA TTAAAATTCT TGCACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221614.1
CDS	112..1791
Translation	MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRACQRNDSDAEAQGKSSEASLTSSVTSLDSSPVELTPRPGSHTIEFFEMCANLIKILAQ

Target ORF information:

RefSeq Version	XM_005221557.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221557.3

ORF Insert Sequence:

ATGCGCAGAC TCAGTTCCTG GAGAAAGATG GCGACAGCCG AGAAGCAGAA ACACGACGGG 
CGGGTGAAGA TCGGTCACTA CATCCTGGGG GATACGCTGG GGGTCGGGAC CTTCGGCAAA 
GTGAAGGTTG GCAAACATGA ATTGACTGGG CATAAAGTTG CTGTGAAGAT ACTGAATCGG 
CAGAAGATTC GAAGCCTTGA TGTAGTAGGA AAAATCCGCA GAGAAATTCA GAACCTCAAG 
CTTTTCAGGC ATCCTCATAT AATTAAACTG TACCAGGTCA TCAGTACACC ATCTGATATT 
TTCATGGTGA TGGAATATGT CTCAGGAGGA GAGCTATTTG ATTATATCTG TAAAAATGGA 
AGGCTGGATG AAAAAGAAAG TCGACGACTG TTCCAACAAA TCCTTTCTGG TGTGGATTAC 
TGTCACAGGC ATATGGTGGT CCACAGAGAT TTGAAACCTG AAAACGTCCT GCTTGATGCA 
CACATGAATG CAAAGATAGC TGATTTTGGT CTTTCAAACA TGATGTCAGA TGGTGAATTT 
TTAAGAACAA GTTGTGGCTC ACCCAACTAT GCTGCACCAG AAGTAATTTC AGGAAGATTG 
TATGCTGGTC CAGAGGTAGA TATATGGAGC AGTGGGGTTA TTCTGTATGC TTTATTATGT 
GGAACCCTTC CGTTTGATGA TGATCACGTG CCAACTCTTT TTAAGAAGAT ATGTGATGGG 
ATCTTTTATA CCCCTCAATA TTTAAATCCT TCTGTGATTA GCCTTTTGAA ACATATGCTG 
CAGGTGGATC CCATGAAGAG AGCCACAATC AAAGATATCA GGGAACATGA ATGGTTTAAA 
CAGGACCTTC CAAAATATCT CTTTCCTGAG GATCCATCAT ATAGCTCAAC CATGATTGAT 
GATGAAGCCT TAAAAGAAGT ATGTGAAAAA TTTGAATGCT CAGAGGAGGA GGTTCTCAGC 
TGCCTTTATA ACAGAAATCA CCAGGACCCT TTGGCAGTTG CCTACCACCT CATAATAGAT 
AACAGGAGGA TAATGAACGA GGCCAAAGAT TTTTACTTGG CAACGAGCCC ACCAGATTCG 
TTTCTCGACG ATCACCACTT GACTCGGCCC CATCCTGAAA GAGTACCATT CTTGGTTGCT 
GAAACTCCCA GGGCGCGCCA TACCCTTGAT GAATTAAATC CACAGAAATC CAAACACCAA 
GGTGTAAGGA AAGCAAAATG GCATTTGGGA ATTAGAAGTC AAAGTCGGCC AAATGATATC 
ATGGCAGAAG TTTGTAGAGC AATTAAACAG CTGGATTATG AATGGAAGGT TGTAAACCCG 
TATTATTTGC GTGTTCGGAG GAAGAATCCT GTGACAAGTA CATACTCCAA AATGAGTCTG 
CAGTTATACC AAGTGGATAG TAGAACGTAT TTACTGGATT TCCGAAGTAT TGATGATGAA 
ATTACTGAAG CCAAATCAGG GACTGCTACG CCACAGAGAT CGGGCTCAGT TAGCAACTAT 
CGAGCTTGCC AAAGGAATGA TTCAGATGCT GAGGCTCAAG GAAAATCCTC AGAAGCTTCT 
CTTACCTCAT CTGTAACCTC ACTTGACTCT TCTCCTGTTG AATTAACTCC AAGACCTGGA 
AGTCACACAA TAGAATTTTT TGAGATGTGC GCGAATCTAA TTAAAATTCT TGCACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA1 ( XM_010816788.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010815090.1 Bos taurus protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant X2, mRNA.

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CloneID	OBa40068
Clone ID Related Accession (Same CDS sequence)	NM_001109802.2 , XM_010816788.2
Accession Version	XM_010816788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104514.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATGG AATATGTCTC AGGAGGAGAG CTATTTGATT ATATCTGTAA AAATGGAAGG 
CTGGATGAAA AAGAAAGTCG ACGACTGTTC CAACAAATCC TTTCTGGTGT GGATTACTGT 
CACAGGCATA TGGTGGTCCA CAGAGATTTG AAACCTGAAA ACGTCCTGCT TGATGCACAC 
ATGAATGCAA AGATAGCTGA TTTTGGTCTT TCAAACATGA TGTCAGATGG TGAATTTTTA 
AGAACAAGTT GTGGCTCACC CAACTATGCT GCACCAGAAG TAATTTCAGG AAGATTGTAT 
GCTGGTCCAG AGGTAGATAT ATGGAGCAGT GGGGTTATTC TGTATGCTTT ATTATGTGGA 
ACCCTTCCGT TTGATGATGA TCACGTGCCA ACTCTTTTTA AGAAGATATG TGATGGGATC 
TTTTATACCC CTCAATATTT AAATCCTTCT GTGATTAGCC TTTTGAAACA TATGCTGCAG 
GTGGATCCCA TGAAGAGAGC CACAATCAAA GATATCAGGG AACATGAATG GTTTAAACAG 
GACCTTCCAA AATATCTCTT TCCTGAGGAT CCATCATATA GCTCAACCAT GATTGATGAT 
GAAGCCTTAA AAGAAGTATG TGAAAAATTT GAATGCTCAG AGGAGGAGGT TCTCAGCTGC 
CTTTATAACA GAAATCACCA GGACCCTTTG GCAGTTGCCT ACCACCTCAT AATAGATAAC 
AGGAGGATAA TGAACGAGGC CAAAGATTTT TACTTGGCAA CGAGCCCACC AGATTCGTTT 
CTCGACGATC ACCACTTGAC TCGGCCCCAT CCTGAAAGAG TACCATTCTT GGTTGCTGAA 
ACTCCCAGGG CGCGCCATAC CCTTGATGAA TTAAATCCAC AGAAATCCAA ACACCAAGGT 
GTAAGGAAAG CAAAATGGCA TTTGGGAATT AGAAGTCAAA GTCGGCCAAA TGATATCATG 
GCAGAAGTTT GTAGAGCAAT TAAACAGCTG GATTATGAAT GGAAGGTTGT AAACCCGTAT 
TATTTGCGTG TTCGGAGGAA GAATCCTGTG ACAAGTACAT ACTCCAAAAT GAGTCTGCAG 
TTATACCAAG TGGATAGTAG AACGTATTTA CTGGATTTCC GAAGTATTGA TGATGAAATT 
ACTGAAGCCA AATCAGGGAC TGCTACGCCA CAGAGATCGG GCTCAGTTAG CAACTATCGA 
GCTTGCCAAA GGAATGATTC AGATGCTGAG GCTCAAGGAA AATCCTCAGA AGCTTCTCTT 
ACCTCATCTG TAACCTCACT TGACTCTTCT CCTGTTGAAT TAACTCCAAG ACCTGGAAGT 
CACACAATAG AATTTTTTGA GATGTGCGCG AATCTAATTA AAATTCTTGC ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010815090.1
CDS	409..1785
Translation	MVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRACQRNDSDAEAQGKSSEASLTSSVTSLDSSPVELTPRPGSHTIEFFEMCANLIKILAQ

Target ORF information:

RefSeq Version	XM_010816788.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816788.2

ORF Insert Sequence:

ATGGTGATGG AATATGTCTC AGGAGGAGAG CTATTTGATT ATATCTGTAA AAATGGAAGG 
CTGGATGAAA AAGAAAGTCG ACGACTGTTC CAACAAATCC TTTCTGGTGT GGATTACTGT 
CACAGGCATA TGGTGGTCCA CAGAGATTTG AAACCTGAAA ACGTCCTGCT TGATGCACAC 
ATGAATGCAA AGATAGCTGA TTTTGGTCTT TCAAACATGA TGTCAGATGG TGAATTTTTA 
AGAACAAGTT GTGGCTCACC CAACTATGCT GCACCAGAAG TAATTTCAGG AAGATTGTAT 
GCTGGTCCAG AGGTAGATAT ATGGAGCAGT GGGGTTATTC TGTATGCTTT ATTATGTGGA 
ACCCTTCCGT TTGATGATGA TCACGTGCCA ACTCTTTTTA AGAAGATATG TGATGGGATC 
TTTTATACCC CTCAATATTT AAATCCTTCT GTGATTAGCC TTTTGAAACA TATGCTGCAG 
GTGGATCCCA TGAAGAGAGC CACAATCAAA GATATCAGGG AACATGAATG GTTTAAACAG 
GACCTTCCAA AATATCTCTT TCCTGAGGAT CCATCATATA GCTCAACCAT GATTGATGAT 
GAAGCCTTAA AAGAAGTATG TGAAAAATTT GAATGCTCAG AGGAGGAGGT TCTCAGCTGC 
CTTTATAACA GAAATCACCA GGACCCTTTG GCAGTTGCCT ACCACCTCAT AATAGATAAC 
AGGAGGATAA TGAACGAGGC CAAAGATTTT TACTTGGCAA CGAGCCCACC AGATTCGTTT 
CTCGACGATC ACCACTTGAC TCGGCCCCAT CCTGAAAGAG TACCATTCTT GGTTGCTGAA 
ACTCCCAGGG CGCGCCATAC CCTTGATGAA TTAAATCCAC AGAAATCCAA ACACCAAGGT 
GTAAGGAAAG CAAAATGGCA TTTGGGAATT AGAAGTCAAA GTCGGCCAAA TGATATCATG 
GCAGAAGTTT GTAGAGCAAT TAAACAGCTG GATTATGAAT GGAAGGTTGT AAACCCGTAT 
TATTTGCGTG TTCGGAGGAA GAATCCTGTG ACAAGTACAT ACTCCAAAAT GAGTCTGCAG 
TTATACCAAG TGGATAGTAG AACGTATTTA CTGGATTTCC GAAGTATTGA TGATGAAATT 
ACTGAAGCCA AATCAGGGAC TGCTACGCCA CAGAGATCGG GCTCAGTTAG CAACTATCGA 
GCTTGCCAAA GGAATGATTC AGATGCTGAG GCTCAAGGAA AATCCTCAGA AGCTTCTCTT 
ACCTCATCTG TAACCTCACT TGACTCTTCT CCTGTTGAAT TAACTCCAAG ACCTGGAAGT 
CACACAATAG AATTTTTTGA GATGTGCGCG AATCTAATTA AAATTCTTGC ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA1 ( XM_024981363.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837131.1 Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa228063
Clone ID Related Accession (Same CDS sequence)	XM_024981363.1 , XM_024981362.1
Accession Version	XM_024981363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCC ACAGAGATTT GAAACCTGAA AACGTCCTGC TTGATGCACA CATGAATGCA 
AAGATAGCTG ATTTTGGTCT TTCAAACATG ATGTCAGATG GTGAATTTTT AAGAACAAGT 
TGTGGCTCAC CCAACTATGC TGCACCAGAA GTAATTTCAG GAAGATTGTA TGCTGGTCCA 
GAGGTAGATA TATGGAGCAG TGGGGTTATT CTGTATGCTT TATTATGTGG AACCCTTCCG 
TTTGATGATG ATCACGTGCC AACTCTTTTT AAGAAGATAT GTGATGGGAT CTTTTATACC 
CCTCAATATT TAAATCCTTC TGTGATTAGC CTTTTGAAAC ATATGCTGCA GGTGGATCCC 
ATGAAGAGAG CCACAATCAA AGATATCAGG GAACATGAAT GGTTTAAACA GGACCTTCCA 
AAATATCTCT TTCCTGAGGA TCCATCATAT AGCTCAACCA TGATTGATGA TGAAGCCTTA 
AAAGAAGTAT GTGAAAAATT TGAATGCTCA GAGGAGGAGG TTCTCAGCTG CCTTTATAAC 
AGAAATCACC AGGACCCTTT GGCAGTTGCC TACCACCTCA TAATAGATAA CAGGAGGATA 
ATGAACGAGG CCAAAGATTT TTACTTGGCA ACGAGCCCAC CAGATTCGTT TCTCGACGAT 
CACCACTTGA CTCGGCCCCA TCCTGAAAGA GTACCATTCT TGGTTGCTGA AACTCCCAGG 
GCGCGCCATA CCCTTGATGA ATTAAATCCA CAGAAATCCA AACACCAAGG TGTAAGGAAA 
GCAAAATGGC ATTTGGGAAT TAGAAGTCAA AGTCGGCCAA ATGATATCAT GGCAGAAGTT 
TGTAGAGCAA TTAAACAGCT GGATTATGAA TGGAAGGTTG TAAACCCGTA TTATTTGCGT 
GTTCGGAGGA AGAATCCTGT GACAAGTACA TACTCCAAAA TGAGTCTGCA GTTATACCAA 
GTGGATAGTA GAACGTATTT ACTGGATTTC CGAAGTATTG ATGATGAAAT TACTGAAGCC 
AAATCAGGGA CTGCTACGCC ACAGAGATCG GGCTCAGTTA GCAACTATCG AGCTTGCCAA 
AGGAATGATT CAGATGCTGA GGCTCAAGGA AAATCCTCAG AAGCTTCTCT TACCTCATCT 
GTAACCTCAC TTGACTCTTC TCCTGTTGAA TTAACTCCAA GACCTGGAAG TCACACAATA 
GAATTTTTTG AGATGTGCGC GAATCTAATT AAAATTCTTG CACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837131.1
CDS	319..1566
Translation	MVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRACQRNDSDAEAQGKSSEASLTSSVTSLDSSPVELTPRPGSHTIEFFEMCANLIKILAQ

Target ORF information:

RefSeq Version	XM_024981363.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981363.1

ORF Insert Sequence:

ATGGTGGTCC ACAGAGATTT GAAACCTGAA AACGTCCTGC TTGATGCACA CATGAATGCA 
AAGATAGCTG ATTTTGGTCT TTCAAACATG ATGTCAGATG GTGAATTTTT AAGAACAAGT 
TGTGGCTCAC CCAACTATGC TGCACCAGAA GTAATTTCAG GAAGATTGTA TGCTGGTCCA 
GAGGTAGATA TATGGAGCAG TGGGGTTATT CTGTATGCTT TATTATGTGG AACCCTTCCG 
TTTGATGATG ATCACGTGCC AACTCTTTTT AAGAAGATAT GTGATGGGAT CTTTTATACC 
CCTCAATATT TAAATCCTTC TGTGATTAGC CTTTTGAAAC ATATGCTGCA GGTGGATCCC 
ATGAAGAGAG CCACAATCAA AGATATCAGG GAACATGAAT GGTTTAAACA GGACCTTCCA 
AAATATCTCT TTCCTGAGGA TCCATCATAT AGCTCAACCA TGATTGATGA TGAAGCCTTA 
AAAGAAGTAT GTGAAAAATT TGAATGCTCA GAGGAGGAGG TTCTCAGCTG CCTTTATAAC 
AGAAATCACC AGGACCCTTT GGCAGTTGCC TACCACCTCA TAATAGATAA CAGGAGGATA 
ATGAACGAGG CCAAAGATTT TTACTTGGCA ACGAGCCCAC CAGATTCGTT TCTCGACGAT 
CACCACTTGA CTCGGCCCCA TCCTGAAAGA GTACCATTCT TGGTTGCTGA AACTCCCAGG 
GCGCGCCATA CCCTTGATGA ATTAAATCCA CAGAAATCCA AACACCAAGG TGTAAGGAAA 
GCAAAATGGC ATTTGGGAAT TAGAAGTCAA AGTCGGCCAA ATGATATCAT GGCAGAAGTT 
TGTAGAGCAA TTAAACAGCT GGATTATGAA TGGAAGGTTG TAAACCCGTA TTATTTGCGT 
GTTCGGAGGA AGAATCCTGT GACAAGTACA TACTCCAAAA TGAGTCTGCA GTTATACCAA 
GTGGATAGTA GAACGTATTT ACTGGATTTC CGAAGTATTG ATGATGAAAT TACTGAAGCC 
AAATCAGGGA CTGCTACGCC ACAGAGATCG GGCTCAGTTA GCAACTATCG AGCTTGCCAA 
AGGAATGATT CAGATGCTGA GGCTCAAGGA AAATCCTCAG AAGCTTCTCT TACCTCATCT 
GTAACCTCAC TTGACTCTTC TCCTGTTGAA TTAACTCCAA GACCTGGAAG TCACACAATA 
GAATTTTTTG AGATGTGCGC GAATCTAATT AAAATTCTTG CACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA1 ( XM_024981362.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837130.1 Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa228063
Clone ID Related Accession (Same CDS sequence)	XM_024981363.1 , XM_024981362.1
Accession Version	XM_024981362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCC ACAGAGATTT GAAACCTGAA AACGTCCTGC TTGATGCACA CATGAATGCA 
AAGATAGCTG ATTTTGGTCT TTCAAACATG ATGTCAGATG GTGAATTTTT AAGAACAAGT 
TGTGGCTCAC CCAACTATGC TGCACCAGAA GTAATTTCAG GAAGATTGTA TGCTGGTCCA 
GAGGTAGATA TATGGAGCAG TGGGGTTATT CTGTATGCTT TATTATGTGG AACCCTTCCG 
TTTGATGATG ATCACGTGCC AACTCTTTTT AAGAAGATAT GTGATGGGAT CTTTTATACC 
CCTCAATATT TAAATCCTTC TGTGATTAGC CTTTTGAAAC ATATGCTGCA GGTGGATCCC 
ATGAAGAGAG CCACAATCAA AGATATCAGG GAACATGAAT GGTTTAAACA GGACCTTCCA 
AAATATCTCT TTCCTGAGGA TCCATCATAT AGCTCAACCA TGATTGATGA TGAAGCCTTA 
AAAGAAGTAT GTGAAAAATT TGAATGCTCA GAGGAGGAGG TTCTCAGCTG CCTTTATAAC 
AGAAATCACC AGGACCCTTT GGCAGTTGCC TACCACCTCA TAATAGATAA CAGGAGGATA 
ATGAACGAGG CCAAAGATTT TTACTTGGCA ACGAGCCCAC CAGATTCGTT TCTCGACGAT 
CACCACTTGA CTCGGCCCCA TCCTGAAAGA GTACCATTCT TGGTTGCTGA AACTCCCAGG 
GCGCGCCATA CCCTTGATGA ATTAAATCCA CAGAAATCCA AACACCAAGG TGTAAGGAAA 
GCAAAATGGC ATTTGGGAAT TAGAAGTCAA AGTCGGCCAA ATGATATCAT GGCAGAAGTT 
TGTAGAGCAA TTAAACAGCT GGATTATGAA TGGAAGGTTG TAAACCCGTA TTATTTGCGT 
GTTCGGAGGA AGAATCCTGT GACAAGTACA TACTCCAAAA TGAGTCTGCA GTTATACCAA 
GTGGATAGTA GAACGTATTT ACTGGATTTC CGAAGTATTG ATGATGAAAT TACTGAAGCC 
AAATCAGGGA CTGCTACGCC ACAGAGATCG GGCTCAGTTA GCAACTATCG AGCTTGCCAA 
AGGAATGATT CAGATGCTGA GGCTCAAGGA AAATCCTCAG AAGCTTCTCT TACCTCATCT 
GTAACCTCAC TTGACTCTTC TCCTGTTGAA TTAACTCCAA GACCTGGAAG TCACACAATA 
GAATTTTTTG AGATGTGCGC GAATCTAATT AAAATTCTTG CACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837130.1
CDS	451..1698
Translation	MVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRACQRNDSDAEAQGKSSEASLTSSVTSLDSSPVELTPRPGSHTIEFFEMCANLIKILAQ

Target ORF information:

RefSeq Version	XM_024981362.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981362.1

ORF Insert Sequence:

ATGGTGGTCC ACAGAGATTT GAAACCTGAA AACGTCCTGC TTGATGCACA CATGAATGCA 
AAGATAGCTG ATTTTGGTCT TTCAAACATG ATGTCAGATG GTGAATTTTT AAGAACAAGT 
TGTGGCTCAC CCAACTATGC TGCACCAGAA GTAATTTCAG GAAGATTGTA TGCTGGTCCA 
GAGGTAGATA TATGGAGCAG TGGGGTTATT CTGTATGCTT TATTATGTGG AACCCTTCCG 
TTTGATGATG ATCACGTGCC AACTCTTTTT AAGAAGATAT GTGATGGGAT CTTTTATACC 
CCTCAATATT TAAATCCTTC TGTGATTAGC CTTTTGAAAC ATATGCTGCA GGTGGATCCC 
ATGAAGAGAG CCACAATCAA AGATATCAGG GAACATGAAT GGTTTAAACA GGACCTTCCA 
AAATATCTCT TTCCTGAGGA TCCATCATAT AGCTCAACCA TGATTGATGA TGAAGCCTTA 
AAAGAAGTAT GTGAAAAATT TGAATGCTCA GAGGAGGAGG TTCTCAGCTG CCTTTATAAC 
AGAAATCACC AGGACCCTTT GGCAGTTGCC TACCACCTCA TAATAGATAA CAGGAGGATA 
ATGAACGAGG CCAAAGATTT TTACTTGGCA ACGAGCCCAC CAGATTCGTT TCTCGACGAT 
CACCACTTGA CTCGGCCCCA TCCTGAAAGA GTACCATTCT TGGTTGCTGA AACTCCCAGG 
GCGCGCCATA CCCTTGATGA ATTAAATCCA CAGAAATCCA AACACCAAGG TGTAAGGAAA 
GCAAAATGGC ATTTGGGAAT TAGAAGTCAA AGTCGGCCAA ATGATATCAT GGCAGAAGTT 
TGTAGAGCAA TTAAACAGCT GGATTATGAA TGGAAGGTTG TAAACCCGTA TTATTTGCGT 
GTTCGGAGGA AGAATCCTGT GACAAGTACA TACTCCAAAA TGAGTCTGCA GTTATACCAA 
GTGGATAGTA GAACGTATTT ACTGGATTTC CGAAGTATTG ATGATGAAAT TACTGAAGCC 
AAATCAGGGA CTGCTACGCC ACAGAGATCG GGCTCAGTTA GCAACTATCG AGCTTGCCAA 
AGGAATGATT CAGATGCTGA GGCTCAAGGA AAATCCTCAG AAGCTTCTCT TACCTCATCT 
GTAACCTCAC TTGACTCTTC TCCTGTTGAA TTAACTCCAA GACCTGGAAG TCACACAATA 
GAATTTTTTG AGATGTGCGC GAATCTAATT AAAATTCTTG CACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKAA1 ( XM_024981361.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837129.1 Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X1, mRNA.

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CloneID	OBa228062
Clone ID Related Accession (Same CDS sequence)	XM_024981361.1
Accession Version	XM_024981361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGAC TCAGTTCCTG GAGAAAGATG GCGACAGCCG AGAAGCAGAA ACACGACGGG 
CGGGTGAAGA TCGGTCACTA CATCCTGGGG GATACGCTGG GGGTCGGGAC CTTCGGCAAA 
GTGAAGGTTG GCAAACATGA ATTGACTGGG CATAAAGTTG CTGTGAAGAT ACTGAATCGG 
CAGAAGATTC GAAGCCTTGA TGTAGTAGGA AAAATCCGCA GAGAAATTCA GAACCTCAAG 
CTTTTCAGGC ATCCTCATAT AATTAAACTG TACCAGGTCA TCAGTACACC ATCTGATATT 
TTCATGGTGA TGGAATATGT CTCAGGAGGA GAGCTATTTG ATTATATCTG TAAAAATGGA 
AGGAAATCTG ATGTACCTGG AGTAGAAAGA ACAGGCTCCA TGAAGGAGCT GGATGAAAAA 
GAAAGTCGAC GACTGTTCCA ACAAATCCTT TCTGGTGTGG ATTACTGTCA CAGGCATATG 
GTGGTCCACA GAGATTTGAA ACCTGAAAAC GTCCTGCTTG ATGCACACAT GAATGCAAAG 
ATAGCTGATT TTGGTCTTTC AAACATGATG TCAGATGGTG AATTTTTAAG AACAAGTTGT 
GGCTCACCCA ACTATGCTGC ACCAGAAGTA ATTTCAGGAA GATTGTATGC TGGTCCAGAG 
GTAGATATAT GGAGCAGTGG GGTTATTCTG TATGCTTTAT TATGTGGAAC CCTTCCGTTT 
GATGATGATC ACGTGCCAAC TCTTTTTAAG AAGATATGTG ATGGGATCTT TTATACCCCT 
CAATATTTAA ATCCTTCTGT GATTAGCCTT TTGAAACATA TGCTGCAGGT GGATCCCATG 
AAGAGAGCCA CAATCAAAGA TATCAGGGAA CATGAATGGT TTAAACAGGA CCTTCCAAAA 
TATCTCTTTC CTGAGGATCC ATCATATAGC TCAACCATGA TTGATGATGA AGCCTTAAAA 
GAAGTATGTG AAAAATTTGA ATGCTCAGAG GAGGAGGTTC TCAGCTGCCT TTATAACAGA 
AATCACCAGG ACCCTTTGGC AGTTGCCTAC CACCTCATAA TAGATAACAG GAGGATAATG 
AACGAGGCCA AAGATTTTTA CTTGGCAACG AGCCCACCAG ATTCGTTTCT CGACGATCAC 
CACTTGACTC GGCCCCATCC TGAAAGAGTA CCATTCTTGG TTGCTGAAAC TCCCAGGGCG 
CGCCATACCC TTGATGAATT AAATCCACAG AAATCCAAAC ACCAAGGTGT AAGGAAAGCA 
AAATGGCATT TGGGAATTAG AAGTCAAAGT CGGCCAAATG ATATCATGGC AGAAGTTTGT 
AGAGCAATTA AACAGCTGGA TTATGAATGG AAGGTTGTAA ACCCGTATTA TTTGCGTGTT 
CGGAGGAAGA ATCCTGTGAC AAGTACATAC TCCAAAATGA GTCTGCAGTT ATACCAAGTG 
GATAGTAGAA CGTATTTACT GGATTTCCGA AGTATTGATG ATGAAATTAC TGAAGCCAAA 
TCAGGGACTG CTACGCCACA GAGATCGGGC TCAGTTAGCA ACTATCGAGC TTGCCAAAGG 
AATGATTCAG ATGCTGAGGC TCAAGGAAAA TCCTCAGAAG CTTCTCTTAC CTCATCTGTA 
ACCTCACTTG ACTCTTCTCC TGTTGAATTA ACTCCAAGAC CTGGAAGTCA CACAATAGAA 
TTTTTTGAGA TGTGCGCGAA TCTAATTAAA ATTCTTGCAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837129.1
CDS	19..1743
Translation	MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRKSDVPGVERTGSMKELDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMIDDEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDSFLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDEITEAKSGTATPQRSGSVSNYRACQRNDSDAEAQGKSSEASLTSSVTSLDSSPVELTPRPGSHTIEFFEMCANLIKILAQ

Target ORF information:

RefSeq Version	XM_024981361.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase AMP-activated catalytic subunit alpha 1 (PRKAA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981361.1

ORF Insert Sequence:

ATGCGCAGAC TCAGTTCCTG GAGAAAGATG GCGACAGCCG AGAAGCAGAA ACACGACGGG 
CGGGTGAAGA TCGGTCACTA CATCCTGGGG GATACGCTGG GGGTCGGGAC CTTCGGCAAA 
GTGAAGGTTG GCAAACATGA ATTGACTGGG CATAAAGTTG CTGTGAAGAT ACTGAATCGG 
CAGAAGATTC GAAGCCTTGA TGTAGTAGGA AAAATCCGCA GAGAAATTCA GAACCTCAAG 
CTTTTCAGGC ATCCTCATAT AATTAAACTG TACCAGGTCA TCAGTACACC ATCTGATATT 
TTCATGGTGA TGGAATATGT CTCAGGAGGA GAGCTATTTG ATTATATCTG TAAAAATGGA 
AGGAAATCTG ATGTACCTGG AGTAGAAAGA ACAGGCTCCA TGAAGGAGCT GGATGAAAAA 
GAAAGTCGAC GACTGTTCCA ACAAATCCTT TCTGGTGTGG ATTACTGTCA CAGGCATATG 
GTGGTCCACA GAGATTTGAA ACCTGAAAAC GTCCTGCTTG ATGCACACAT GAATGCAAAG 
ATAGCTGATT TTGGTCTTTC AAACATGATG TCAGATGGTG AATTTTTAAG AACAAGTTGT 
GGCTCACCCA ACTATGCTGC ACCAGAAGTA ATTTCAGGAA GATTGTATGC TGGTCCAGAG 
GTAGATATAT GGAGCAGTGG GGTTATTCTG TATGCTTTAT TATGTGGAAC CCTTCCGTTT 
GATGATGATC ACGTGCCAAC TCTTTTTAAG AAGATATGTG ATGGGATCTT TTATACCCCT 
CAATATTTAA ATCCTTCTGT GATTAGCCTT TTGAAACATA TGCTGCAGGT GGATCCCATG 
AAGAGAGCCA CAATCAAAGA TATCAGGGAA CATGAATGGT TTAAACAGGA CCTTCCAAAA 
TATCTCTTTC CTGAGGATCC ATCATATAGC TCAACCATGA TTGATGATGA AGCCTTAAAA 
GAAGTATGTG AAAAATTTGA ATGCTCAGAG GAGGAGGTTC TCAGCTGCCT TTATAACAGA 
AATCACCAGG ACCCTTTGGC AGTTGCCTAC CACCTCATAA TAGATAACAG GAGGATAATG 
AACGAGGCCA AAGATTTTTA CTTGGCAACG AGCCCACCAG ATTCGTTTCT CGACGATCAC 
CACTTGACTC GGCCCCATCC TGAAAGAGTA CCATTCTTGG TTGCTGAAAC TCCCAGGGCG 
CGCCATACCC TTGATGAATT AAATCCACAG AAATCCAAAC ACCAAGGTGT AAGGAAAGCA 
AAATGGCATT TGGGAATTAG AAGTCAAAGT CGGCCAAATG ATATCATGGC AGAAGTTTGT 
AGAGCAATTA AACAGCTGGA TTATGAATGG AAGGTTGTAA ACCCGTATTA TTTGCGTGTT 
CGGAGGAAGA ATCCTGTGAC AAGTACATAC TCCAAAATGA GTCTGCAGTT ATACCAAGTG 
GATAGTAGAA CGTATTTACT GGATTTCCGA AGTATTGATG ATGAAATTAC TGAAGCCAAA 
TCAGGGACTG CTACGCCACA GAGATCGGGC TCAGTTAGCA ACTATCGAGC TTGCCAAAGG 
AATGATTCAG ATGCTGAGGC TCAAGGAAAA TCCTCAGAAG CTTCTCTTAC CTCATCTGTA 
ACCTCACTTG ACTCTTCTCC TGTTGAATTA ACTCCAAGAC CTGGAAGTCA CACAATAGAA 
TTTTTTGAGA TGTGCGCGAA TCTAATTAAA ATTCTTGCAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucose Availability and AMP-Activated Protein Kinase Link Energy Metabolism and Innate Immunity in the Bovine Endometrium.
PloS one11(3)e0151416(2016)
Turner ML,Cronin JG,Noleto PG,Sheldon IM

Energy depletion of bovine mammary epithelial cells activates AMPK and suppresses protein synthesis through inhibition of mTORC1 signaling.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(3)183-9(2013 Mar)
Burgos SA,Kim JJ,Dai M,Cant JP

Activation of the AMP-activated protein kinase (AMPK) by nitrated lipids in endothelial cells.
PloS one7(2)e31056(2012)
Wu Y,Dong Y,Song P,Zou MH

Ischemia-induced stimulation of Na-K-Cl cotransport in cerebral microvascular endothelial cells involves AMP kinase.
American journal of physiology. Cell physiology301(2)C316-26(2011 Aug)
Wallace BK,Foroutan S,O'Donnell ME

Redox regulation of the AMP-activated protein kinase.
PloS one5(11)e15420(2010 Nov)
Han Y,Wang Q,Song P,Zhu Y,Zou MH

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PRKAA1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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