PUM2 cDNA ORF clone, Bos taurus(cattle)

The following PUM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PUM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa06993 XM_005213099.4
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06993 XM_005193972.3
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06994 XM_024999527.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06994 XM_024999525.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06994 XM_024999526.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06994 XM_864456.8
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06994 XM_003586651.5
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06995 XM_005213100.4
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06995 XM_005193973.3
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa54578 XM_010817483.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa54578 XM_010810300.2
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06990 XM_005193965.3
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_005213092.4
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_015465435.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_015465434.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_015473569.2
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_005193969.3
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_024999522.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_005213096.4
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_015473570.2
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06991 XM_005193970.3
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06991 XM_024999523.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06991 XM_005213097.4
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06991 XM_024999524.1
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06992 XM_010810299.2
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06992 XM_010810298.2
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06992 XM_010817472.2
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06992 XM_010817457.2
Latest version!
Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OBa06994 XM_003586651.4 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06990 XM_005213096.3 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06990 XM_015473569.1 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06995 XM_005213100.3 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa54578 XM_010810300.1 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06990 XM_005213092.3 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06993 XM_005213099.3 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa06990 XM_015473570.1 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa06991 XM_005213097.3 Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa06993
    Clone ID Related Accession (Same CDS sequence) XM_005193972.3 , XM_005213099.3 , XM_005213099.4
    Accession Version XM_005193972.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGGTAATT AGTGAAATGG TAAAGGAGCT CGATGGTCAT
    GTACTCAAAT GTGTGAAAGA TCAAAATGGA AACCATGTTG TACAAAAGTG TATTGAATGT
    GTTCAGCCAC AGTCACTACA GTTCATCATT GATGCTTTCA AAGGACAAGT ATTTGTACTT
    TCAACTCATC CTTATGGCTG CAGAGTAATC CAGCGCATTC TGGAGCATTG CACTGCAGAG
    CAGACCTTAC CTATCTTAGA AGAACTCCAC CAACATACGG AGCAGTTGGT ACAGGATCAG
    TATGGCAATT ACGTTATTCA GCATGTACTG GAACATGGTC GACCTGAAGA CAAGAGCAAA
    ATTGTTTCTG AAATCCGAGG AAAGGTCTTA GCCTTGAGTC AACACAAATT TGCCAGCAAT
    GTAGTAGAAA AGTGTGTTAC TCATGCCTCC CGTGCTGAGA GAGCTTTACT GATTGACGAA
    GTTTGCTGCC AGAATGATGG TCCTCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAATTATG TCGTTCAGAA GATGATTGAT ATGGCTGAAC CTGCTCAGAG AAAGATAATC
    ATGCACAAGA TTCGACCTCA CATTTCTACT TTACGCAAAT ATACATATGG GAAGCATATA
    CTGGCCAAGT TGGAAAAATA TTATTTGAAA AATAGCCCAG ATCTAGGGCC TATTGGAGGA
    CCACCAAATG GAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194029.1
    CDS150..3110
    Translation

    Target ORF information:

    RefSeq Version XM_005193972.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193972.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGGTAATT AGTGAAATGG TAAAGGAGCT CGATGGTCAT
    GTACTCAAAT GTGTGAAAGA TCAAAATGGA AACCATGTTG TACAAAAGTG TATTGAATGT
    GTTCAGCCAC AGTCACTACA GTTCATCATT GATGCTTTCA AAGGACAAGT ATTTGTACTT
    TCAACTCATC CTTATGGCTG CAGAGTAATC CAGCGCATTC TGGAGCATTG CACTGCAGAG
    CAGACCTTAC CTATCTTAGA AGAACTCCAC CAACATACGG AGCAGTTGGT ACAGGATCAG
    TATGGCAATT ACGTTATTCA GCATGTACTG GAACATGGTC GACCTGAAGA CAAGAGCAAA
    ATTGTTTCTG AAATCCGAGG AAAGGTCTTA GCCTTGAGTC AACACAAATT TGCCAGCAAT
    GTAGTAGAAA AGTGTGTTAC TCATGCCTCC CGTGCTGAGA GAGCTTTACT GATTGACGAA
    GTTTGCTGCC AGAATGATGG TCCTCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAATTATG TCGTTCAGAA GATGATTGAT ATGGCTGAAC CTGCTCAGAG AAAGATAATC
    ATGCACAAGA TTCGACCTCA CATTTCTACT TTACGCAAAT ATACATATGG GAAGCATATA
    CTGGCCAAGT TGGAAAAATA TTATTTGAAA AATAGCCCAG ATCTAGGGCC TATTGGAGGA
    CCACCAAATG GAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06993
    Clone ID Related Accession (Same CDS sequence) XM_005193972.3 , XM_005213099.3 , XM_005213099.4
    Accession Version XM_005213099.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213099.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGGTAATT AGTGAAATGG TAAAGGAGCT CGATGGTCAT
    GTACTCAAAT GTGTGAAAGA TCAAAATGGA AACCATGTTG TACAAAAGTG TATTGAATGT
    GTTCAGCCAC AGTCACTACA GTTCATCATT GATGCTTTCA AAGGACAAGT ATTTGTACTT
    TCAACTCATC CTTATGGCTG CAGAGTAATC CAGCGCATTC TGGAGCATTG CACTGCAGAG
    CAGACCTTAC CTATCTTAGA AGAACTCCAC CAACATACGG AGCAGTTGGT ACAGGATCAG
    TATGGCAATT ACGTTATTCA GCATGTACTG GAACATGGTC GACCTGAAGA CAAGAGCAAA
    ATTGTTTCTG AAATCCGAGG AAAGGTCTTA GCCTTGAGTC AACACAAATT TGCCAGCAAT
    GTAGTAGAAA AGTGTGTTAC TCATGCCTCC CGTGCTGAGA GAGCTTTACT GATTGACGAA
    GTTTGCTGCC AGAATGATGG TCCTCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAATTATG TCGTTCAGAA GATGATTGAT ATGGCTGAAC CTGCTCAGAG AAAGATAATC
    ATGCACAAGA TTCGACCTCA CATTTCTACT TTACGCAAAT ATACATATGG GAAGCATATA
    CTGGCCAAGT TGGAAAAATA TTATTTGAAA AATAGCCCAG ATCTAGGGCC TATTGGAGGA
    CCACCAAATG GAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213156.1
    CDS150..3110
    Translation

    Target ORF information:

    RefSeq Version XM_005213099.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213099.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGGTAATT AGTGAAATGG TAAAGGAGCT CGATGGTCAT
    GTACTCAAAT GTGTGAAAGA TCAAAATGGA AACCATGTTG TACAAAAGTG TATTGAATGT
    GTTCAGCCAC AGTCACTACA GTTCATCATT GATGCTTTCA AAGGACAAGT ATTTGTACTT
    TCAACTCATC CTTATGGCTG CAGAGTAATC CAGCGCATTC TGGAGCATTG CACTGCAGAG
    CAGACCTTAC CTATCTTAGA AGAACTCCAC CAACATACGG AGCAGTTGGT ACAGGATCAG
    TATGGCAATT ACGTTATTCA GCATGTACTG GAACATGGTC GACCTGAAGA CAAGAGCAAA
    ATTGTTTCTG AAATCCGAGG AAAGGTCTTA GCCTTGAGTC AACACAAATT TGCCAGCAAT
    GTAGTAGAAA AGTGTGTTAC TCATGCCTCC CGTGCTGAGA GAGCTTTACT GATTGACGAA
    GTTTGCTGCC AGAATGATGG TCCTCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAATTATG TCGTTCAGAA GATGATTGAT ATGGCTGAAC CTGCTCAGAG AAAGATAATC
    ATGCACAAGA TTCGACCTCA CATTTCTACT TTACGCAAAT ATACATATGG GAAGCATATA
    CTGGCCAAGT TGGAAAAATA TTATTTGAAA AATAGCCCAG ATCTAGGGCC TATTGGAGGA
    CCACCAAATG GAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06993
    Clone ID Related Accession (Same CDS sequence) XM_005193972.3 , XM_005213099.3 , XM_005213099.4
    Accession Version XM_005213099.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213099.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGGTAATT AGTGAAATGG TAAAGGAGCT CGATGGTCAT
    GTACTCAAAT GTGTGAAAGA TCAAAATGGA AACCATGTTG TACAAAAGTG TATTGAATGT
    GTTCAGCCAC AGTCACTACA GTTCATCATT GATGCTTTCA AAGGACAAGT ATTTGTACTT
    TCAACTCATC CTTATGGCTG CAGAGTAATC CAGCGCATTC TGGAGCATTG CACTGCAGAG
    CAGACCTTAC CTATCTTAGA AGAACTCCAC CAACATACGG AGCAGTTGGT ACAGGATCAG
    TATGGCAATT ACGTTATTCA GCATGTACTG GAACATGGTC GACCTGAAGA CAAGAGCAAA
    ATTGTTTCTG AAATCCGAGG AAAGGTCTTA GCCTTGAGTC AACACAAATT TGCCAGCAAT
    GTAGTAGAAA AGTGTGTTAC TCATGCCTCC CGTGCTGAGA GAGCTTTACT GATTGACGAA
    GTTTGCTGCC AGAATGATGG TCCTCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAATTATG TCGTTCAGAA GATGATTGAT ATGGCTGAAC CTGCTCAGAG AAAGATAATC
    ATGCACAAGA TTCGACCTCA CATTTCTACT TTACGCAAAT ATACATATGG GAAGCATATA
    CTGGCCAAGT TGGAAAAATA TTATTTGAAA AATAGCCCAG ATCTAGGGCC TATTGGAGGA
    CCACCAAATG GAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213156.1
    CDS167..3127
    Translation

    Target ORF information:

    RefSeq Version XM_005213099.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213099.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGGTAATT AGTGAAATGG TAAAGGAGCT CGATGGTCAT
    GTACTCAAAT GTGTGAAAGA TCAAAATGGA AACCATGTTG TACAAAAGTG TATTGAATGT
    GTTCAGCCAC AGTCACTACA GTTCATCATT GATGCTTTCA AAGGACAAGT ATTTGTACTT
    TCAACTCATC CTTATGGCTG CAGAGTAATC CAGCGCATTC TGGAGCATTG CACTGCAGAG
    CAGACCTTAC CTATCTTAGA AGAACTCCAC CAACATACGG AGCAGTTGGT ACAGGATCAG
    TATGGCAATT ACGTTATTCA GCATGTACTG GAACATGGTC GACCTGAAGA CAAGAGCAAA
    ATTGTTTCTG AAATCCGAGG AAAGGTCTTA GCCTTGAGTC AACACAAATT TGCCAGCAAT
    GTAGTAGAAA AGTGTGTTAC TCATGCCTCC CGTGCTGAGA GAGCTTTACT GATTGACGAA
    GTTTGCTGCC AGAATGATGG TCCTCACAGT GCCTTATACA CCATGATGAA GGACCAGTAT
    GCCAATTATG TCGTTCAGAA GATGATTGAT ATGGCTGAAC CTGCTCAGAG AAAGATAATC
    ATGCACAAGA TTCGACCTCA CATTTCTACT TTACGCAAAT ATACATATGG GAAGCATATA
    CTGGCCAAGT TGGAAAAATA TTATTTGAAA AATAGCCCAG ATCTAGGGCC TATTGGAGGA
    CCACCAAATG GAATGCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06994
    Clone ID Related Accession (Same CDS sequence) XM_003586651.5 , XM_003586651.4 , XM_024999526.1 , XM_864456.8 , XM_024999525.1 , XM_024999527.1
    Accession Version XM_003586651.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586651.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586699.1
    CDS272..3226
    Translation

    Target ORF information:

    RefSeq Version XM_003586651.5
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586651.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06994
    Clone ID Related Accession (Same CDS sequence) XM_003586651.5 , XM_003586651.4 , XM_024999526.1 , XM_864456.8 , XM_024999525.1 , XM_024999527.1
    Accession Version XM_003586651.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586651.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586699.1
    CDS150..3104
    Translation

    Target ORF information:

    RefSeq Version XM_003586651.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586651.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06994
    Clone ID Related Accession (Same CDS sequence) XM_003586651.5 , XM_003586651.4 , XM_024999526.1 , XM_864456.8 , XM_024999525.1 , XM_024999527.1
    Accession Version XM_024999526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855294.1
    CDS73..3027
    Translation

    Target ORF information:

    RefSeq Version XM_024999526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06994
    Clone ID Related Accession (Same CDS sequence) XM_003586651.5 , XM_003586651.4 , XM_024999526.1 , XM_864456.8 , XM_024999525.1 , XM_024999527.1
    Accession Version XM_864456.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_864456.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_869549.5
    CDS150..3104
    Translation

    Target ORF information:

    RefSeq Version XM_864456.8
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_864456.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06994
    Clone ID Related Accession (Same CDS sequence) XM_003586651.5 , XM_003586651.4 , XM_024999526.1 , XM_864456.8 , XM_024999525.1 , XM_024999527.1
    Accession Version XM_024999525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855293.1
    CDS58..3012
    Translation

    Target ORF information:

    RefSeq Version XM_024999525.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06994
    Clone ID Related Accession (Same CDS sequence) XM_003586651.5 , XM_003586651.4 , XM_024999526.1 , XM_864456.8 , XM_024999525.1 , XM_024999527.1
    Accession Version XM_024999527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2955bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855295.1
    CDS81..3035
    Translation

    Target ORF information:

    RefSeq Version XM_024999527.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGGAG GACTGACAAA TGGTAGTGGT
    CGATATATCT CTGCAGCACC TGGAGCAGAA GCAAAATACC GAAGTGCTTC AGGCACTTCC
    AGTCTATTTA GCTCCAGCAG CCAGCTCTTT CCTCCTTCTC GGCTTCGGTA TAATAGGTCT
    GATATTATGC CTTCTGGCCG AAGTAGATTA TTAGAAGATT TCAGAAACAA CCGCTTCCCA
    AACCTTCAAC TTAGAGACTT GATTGGACAC ATAGTTGAGT TTTCTCAAGA CCAGCATGGT
    TCTAGATTCA TACAGCAAAA GCTAGAGAGA GCTACTCCAG CTGAGCGACA GATGGTTTTT
    AATGAAATTC TACAAGCAGC CTATCAATTA ATGACAGATG TTTTTGGCAA CTATGTCATA
    CAGAAATTTT TTGAGTTTGG GAGTTTGGAT CAAAAATTAG CCCTAGCTAC TCGTATTCGA
    GGTCATGTTC TACCCTTAGC ATTGCAGATG TATGGCTGCC GTGTTATTCA GAAAGCATTA
    GAGTCTATTT CATCTGACCA GCAGAGTGAA ATGGTAAAGG AGCTCGATGG TCATGTACTC
    AAATGTGTGA AAGATCAAAA TGGAAACCAT GTTGTACAAA AGTGTATTGA ATGTGTTCAG
    CCACAGTCAC TACAGTTCAT CATTGATGCT TTCAAAGGAC AAGTATTTGT ACTTTCAACT
    CATCCTTATG GCTGCAGAGT AATCCAGCGC ATTCTGGAGC ATTGCACTGC AGAGCAGACC
    TTACCTATCT TAGAAGAACT CCACCAACAT ACGGAGCAGT TGGTACAGGA TCAGTATGGC
    AATTACGTTA TTCAGCATGT ACTGGAACAT GGTCGACCTG AAGACAAGAG CAAAATTGTT
    TCTGAAATCC GAGGAAAGGT CTTAGCCTTG AGTCAACACA AATTTGCCAG CAATGTAGTA
    GAAAAGTGTG TTACTCATGC CTCCCGTGCT GAGAGAGCTT TACTGATTGA CGAAGTTTGC
    TGCCAGAATG ATGGTCCTCA CAGTGCCTTA TACACCATGA TGAAGGACCA GTATGCCAAT
    TATGTCGTTC AGAAGATGAT TGATATGGCT GAACCTGCTC AGAGAAAGAT AATCATGCAC
    AAGATTCGAC CTCACATTTC TACTTTACGC AAATATACAT ATGGGAAGCA TATACTGGCC
    AAGTTGGAAA AATATTATTT GAAAAATAGC CCAGATCTAG GGCCTATTGG AGGACCACCA
    AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06995
    Clone ID Related Accession (Same CDS sequence) XM_005193973.3 , XM_005213100.3 , XM_005213100.4
    Accession Version XM_005193973.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193973.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG CTATCAAGTA
    CTAGCTCCAA CTGCCTATTA TGATCAGACC GGTGCCTTAG TGGTTGGCCC TGGGGCAAGA
    ACTGGCCTTG GAGCTCCAGT TCGATTAATG GCTCCAACAC CTGTTTTAAT TAGTTCAGCA
    GCAGCACAAG CTGCAGCGGC AGCAGCAGCT GGAGGAACTG CAAATAGTCT CACAGGCAGC
    ACAAATGGTC TGTTCCGGCC AATTGGCACT CAGCCACCAC CGCAGCAGCA GCAGCAGCAG
    CAGCCAAGCA CTAATCTGCA GTCTAATTCA TTTTATGGGA GCACTTCTCT GACTAATAGC
    TCCCAGAGCA GTTCTTTATT TTCTCACGGA CCTGGCCAGC CTGGAAGTAC ATCTCTTGGC
    TTTGGAAGCA GTAGCTCTTT GGGTGCTGCT ATAGGCTCAG CCCTCAGTGG ATTTGGTTCA
    TCAGTTGGCA GTTCTGCAAG TAGTAGTGCC ACAAGGAGAG AGTCTCTATC TACTAGCTCT
    GACTTGTACA AAAGATCTAG TAGCAGCCTA GCACCCATAG GGCAACCATT TTACAATAGT
    CTGGGATTTT CCTCCTCTCC AAGTCCAATA GGCATGCCTC TGCCAAGCCA AACTCCAGGA
    CATTCACTTA CGCCACCGCC ATCACTTTCA TCACATGGAT CCTCATCCAG TTTGCATTTA
    GGAGGACTGA CAAATGGTAG TGGTCGATAT ATCTCTGCAG CACCTGGAGC AGAAGCAAAA
    TACCGAAGTG CTTCAGGCAC TTCCAGTCTA TTTAGCTCCA GCAGCCAGCT CTTTCCTCCT
    TCTCGGCTTC GGTATAATAG GTCTGATATT ATGCCTTCTG GCCGAAGTAG ATTATTAGAA
    GATTTCAGAA ACAACCGCTT CCCAAACCTT CAACTTAGAG ACTTGATTGG ACACATAGTT
    GAGTTTTCTC AAGACCAGCA TGGTTCTAGA TTCATACAGC AAAAGCTAGA GAGAGCTACT
    CCAGCTGAGC GACAGATGGT TTTTAATGAA ATTCTACAAG CAGCCTATCA ATTAATGACA
    GATGTTTTTG GCAACTATGT CATACAGAAA TTTTTTGAGT TTGGGAGTTT GGATCAAAAA
    TTAGCCCTAG CTACTCGTAT TCGAGGTCAT GTTCTACCCT TAGCATTGCA GATGTATGGC
    TGCCGTGTTA TTCAGAAAGC ATTAGAGTCT ATTTCATCTG ACCAGCAGGT AATTAGTGAA
    ATGGTAAAGG AGCTCGATGG TCATGTACTC AAATGTGTGA AAGATCAAAA TGGAAACCAT
    GTTGTACAAA AGTGTATTGA ATGTGTTCAG CCACAGTCAC TACAGTTCAT CATTGATGCT
    TTCAAAGGAC AAGTATTTGT ACTTTCAACT CATCCTTATG GCTGCAGAGT AATCCAGCGC
    ATTCTGGAGC ATTGCACTGC AGAGCAGACC TTACCTATCT TAGAAGAACT CCACCAACAT
    ACGGAGCAGT TGGTACAGGA TCAGTATGGC AATTACGTTA TTCAGCATGT ACTGGAACAT
    GGTCGACCTG AAGACAAGAG CAAAATTGTT TCTGAAATCC GAGGAAAGGT CTTAGCCTTG
    AGTCAACACA AATTTGCCAG CAATGTAGTA GAAAAGTGTG TTACTCATGC CTCCCGTGCT
    GAGAGAGCTT TACTGATTGA CGAAGTTTGC TGCCAGAATG ATGGTCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAT TATGTCGTTC AGAAGATGAT TGATATGGCT
    GAACCTGCTC AGAGAAAGAT AATCATGCAC AAGATTCGAC CTCACATTTC TACTTTACGC
    AAATATACAT ATGGGAAGCA TATACTGGCC AAGTTGGAAA AATATTATTT GAAAAATAGC
    CCAGATCTAG GGCCTATTGG AGGACCACCA AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194030.1
    CDS150..3074
    Translation

    Target ORF information:

    RefSeq Version XM_005193973.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193973.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG CTATCAAGTA
    CTAGCTCCAA CTGCCTATTA TGATCAGACC GGTGCCTTAG TGGTTGGCCC TGGGGCAAGA
    ACTGGCCTTG GAGCTCCAGT TCGATTAATG GCTCCAACAC CTGTTTTAAT TAGTTCAGCA
    GCAGCACAAG CTGCAGCGGC AGCAGCAGCT GGAGGAACTG CAAATAGTCT CACAGGCAGC
    ACAAATGGTC TGTTCCGGCC AATTGGCACT CAGCCACCAC CGCAGCAGCA GCAGCAGCAG
    CAGCCAAGCA CTAATCTGCA GTCTAATTCA TTTTATGGGA GCACTTCTCT GACTAATAGC
    TCCCAGAGCA GTTCTTTATT TTCTCACGGA CCTGGCCAGC CTGGAAGTAC ATCTCTTGGC
    TTTGGAAGCA GTAGCTCTTT GGGTGCTGCT ATAGGCTCAG CCCTCAGTGG ATTTGGTTCA
    TCAGTTGGCA GTTCTGCAAG TAGTAGTGCC ACAAGGAGAG AGTCTCTATC TACTAGCTCT
    GACTTGTACA AAAGATCTAG TAGCAGCCTA GCACCCATAG GGCAACCATT TTACAATAGT
    CTGGGATTTT CCTCCTCTCC AAGTCCAATA GGCATGCCTC TGCCAAGCCA AACTCCAGGA
    CATTCACTTA CGCCACCGCC ATCACTTTCA TCACATGGAT CCTCATCCAG TTTGCATTTA
    GGAGGACTGA CAAATGGTAG TGGTCGATAT ATCTCTGCAG CACCTGGAGC AGAAGCAAAA
    TACCGAAGTG CTTCAGGCAC TTCCAGTCTA TTTAGCTCCA GCAGCCAGCT CTTTCCTCCT
    TCTCGGCTTC GGTATAATAG GTCTGATATT ATGCCTTCTG GCCGAAGTAG ATTATTAGAA
    GATTTCAGAA ACAACCGCTT CCCAAACCTT CAACTTAGAG ACTTGATTGG ACACATAGTT
    GAGTTTTCTC AAGACCAGCA TGGTTCTAGA TTCATACAGC AAAAGCTAGA GAGAGCTACT
    CCAGCTGAGC GACAGATGGT TTTTAATGAA ATTCTACAAG CAGCCTATCA ATTAATGACA
    GATGTTTTTG GCAACTATGT CATACAGAAA TTTTTTGAGT TTGGGAGTTT GGATCAAAAA
    TTAGCCCTAG CTACTCGTAT TCGAGGTCAT GTTCTACCCT TAGCATTGCA GATGTATGGC
    TGCCGTGTTA TTCAGAAAGC ATTAGAGTCT ATTTCATCTG ACCAGCAGGT AATTAGTGAA
    ATGGTAAAGG AGCTCGATGG TCATGTACTC AAATGTGTGA AAGATCAAAA TGGAAACCAT
    GTTGTACAAA AGTGTATTGA ATGTGTTCAG CCACAGTCAC TACAGTTCAT CATTGATGCT
    TTCAAAGGAC AAGTATTTGT ACTTTCAACT CATCCTTATG GCTGCAGAGT AATCCAGCGC
    ATTCTGGAGC ATTGCACTGC AGAGCAGACC TTACCTATCT TAGAAGAACT CCACCAACAT
    ACGGAGCAGT TGGTACAGGA TCAGTATGGC AATTACGTTA TTCAGCATGT ACTGGAACAT
    GGTCGACCTG AAGACAAGAG CAAAATTGTT TCTGAAATCC GAGGAAAGGT CTTAGCCTTG
    AGTCAACACA AATTTGCCAG CAATGTAGTA GAAAAGTGTG TTACTCATGC CTCCCGTGCT
    GAGAGAGCTT TACTGATTGA CGAAGTTTGC TGCCAGAATG ATGGTCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAT TATGTCGTTC AGAAGATGAT TGATATGGCT
    GAACCTGCTC AGAGAAAGAT AATCATGCAC AAGATTCGAC CTCACATTTC TACTTTACGC
    AAATATACAT ATGGGAAGCA TATACTGGCC AAGTTGGAAA AATATTATTT GAAAAATAGC
    CCAGATCTAG GGCCTATTGG AGGACCACCA AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06995
    Clone ID Related Accession (Same CDS sequence) XM_005193973.3 , XM_005213100.3 , XM_005213100.4
    Accession Version XM_005213100.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG CTATCAAGTA
    CTAGCTCCAA CTGCCTATTA TGATCAGACC GGTGCCTTAG TGGTTGGCCC TGGGGCAAGA
    ACTGGCCTTG GAGCTCCAGT TCGATTAATG GCTCCAACAC CTGTTTTAAT TAGTTCAGCA
    GCAGCACAAG CTGCAGCGGC AGCAGCAGCT GGAGGAACTG CAAATAGTCT CACAGGCAGC
    ACAAATGGTC TGTTCCGGCC AATTGGCACT CAGCCACCAC CGCAGCAGCA GCAGCAGCAG
    CAGCCAAGCA CTAATCTGCA GTCTAATTCA TTTTATGGGA GCACTTCTCT GACTAATAGC
    TCCCAGAGCA GTTCTTTATT TTCTCACGGA CCTGGCCAGC CTGGAAGTAC ATCTCTTGGC
    TTTGGAAGCA GTAGCTCTTT GGGTGCTGCT ATAGGCTCAG CCCTCAGTGG ATTTGGTTCA
    TCAGTTGGCA GTTCTGCAAG TAGTAGTGCC ACAAGGAGAG AGTCTCTATC TACTAGCTCT
    GACTTGTACA AAAGATCTAG TAGCAGCCTA GCACCCATAG GGCAACCATT TTACAATAGT
    CTGGGATTTT CCTCCTCTCC AAGTCCAATA GGCATGCCTC TGCCAAGCCA AACTCCAGGA
    CATTCACTTA CGCCACCGCC ATCACTTTCA TCACATGGAT CCTCATCCAG TTTGCATTTA
    GGAGGACTGA CAAATGGTAG TGGTCGATAT ATCTCTGCAG CACCTGGAGC AGAAGCAAAA
    TACCGAAGTG CTTCAGGCAC TTCCAGTCTA TTTAGCTCCA GCAGCCAGCT CTTTCCTCCT
    TCTCGGCTTC GGTATAATAG GTCTGATATT ATGCCTTCTG GCCGAAGTAG ATTATTAGAA
    GATTTCAGAA ACAACCGCTT CCCAAACCTT CAACTTAGAG ACTTGATTGG ACACATAGTT
    GAGTTTTCTC AAGACCAGCA TGGTTCTAGA TTCATACAGC AAAAGCTAGA GAGAGCTACT
    CCAGCTGAGC GACAGATGGT TTTTAATGAA ATTCTACAAG CAGCCTATCA ATTAATGACA
    GATGTTTTTG GCAACTATGT CATACAGAAA TTTTTTGAGT TTGGGAGTTT GGATCAAAAA
    TTAGCCCTAG CTACTCGTAT TCGAGGTCAT GTTCTACCCT TAGCATTGCA GATGTATGGC
    TGCCGTGTTA TTCAGAAAGC ATTAGAGTCT ATTTCATCTG ACCAGCAGGT AATTAGTGAA
    ATGGTAAAGG AGCTCGATGG TCATGTACTC AAATGTGTGA AAGATCAAAA TGGAAACCAT
    GTTGTACAAA AGTGTATTGA ATGTGTTCAG CCACAGTCAC TACAGTTCAT CATTGATGCT
    TTCAAAGGAC AAGTATTTGT ACTTTCAACT CATCCTTATG GCTGCAGAGT AATCCAGCGC
    ATTCTGGAGC ATTGCACTGC AGAGCAGACC TTACCTATCT TAGAAGAACT CCACCAACAT
    ACGGAGCAGT TGGTACAGGA TCAGTATGGC AATTACGTTA TTCAGCATGT ACTGGAACAT
    GGTCGACCTG AAGACAAGAG CAAAATTGTT TCTGAAATCC GAGGAAAGGT CTTAGCCTTG
    AGTCAACACA AATTTGCCAG CAATGTAGTA GAAAAGTGTG TTACTCATGC CTCCCGTGCT
    GAGAGAGCTT TACTGATTGA CGAAGTTTGC TGCCAGAATG ATGGTCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAT TATGTCGTTC AGAAGATGAT TGATATGGCT
    GAACCTGCTC AGAGAAAGAT AATCATGCAC AAGATTCGAC CTCACATTTC TACTTTACGC
    AAATATACAT ATGGGAAGCA TATACTGGCC AAGTTGGAAA AATATTATTT GAAAAATAGC
    CCAGATCTAG GGCCTATTGG AGGACCACCA AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213157.1
    CDS150..3074
    Translation

    Target ORF information:

    RefSeq Version XM_005213100.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213100.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG CTATCAAGTA
    CTAGCTCCAA CTGCCTATTA TGATCAGACC GGTGCCTTAG TGGTTGGCCC TGGGGCAAGA
    ACTGGCCTTG GAGCTCCAGT TCGATTAATG GCTCCAACAC CTGTTTTAAT TAGTTCAGCA
    GCAGCACAAG CTGCAGCGGC AGCAGCAGCT GGAGGAACTG CAAATAGTCT CACAGGCAGC
    ACAAATGGTC TGTTCCGGCC AATTGGCACT CAGCCACCAC CGCAGCAGCA GCAGCAGCAG
    CAGCCAAGCA CTAATCTGCA GTCTAATTCA TTTTATGGGA GCACTTCTCT GACTAATAGC
    TCCCAGAGCA GTTCTTTATT TTCTCACGGA CCTGGCCAGC CTGGAAGTAC ATCTCTTGGC
    TTTGGAAGCA GTAGCTCTTT GGGTGCTGCT ATAGGCTCAG CCCTCAGTGG ATTTGGTTCA
    TCAGTTGGCA GTTCTGCAAG TAGTAGTGCC ACAAGGAGAG AGTCTCTATC TACTAGCTCT
    GACTTGTACA AAAGATCTAG TAGCAGCCTA GCACCCATAG GGCAACCATT TTACAATAGT
    CTGGGATTTT CCTCCTCTCC AAGTCCAATA GGCATGCCTC TGCCAAGCCA AACTCCAGGA
    CATTCACTTA CGCCACCGCC ATCACTTTCA TCACATGGAT CCTCATCCAG TTTGCATTTA
    GGAGGACTGA CAAATGGTAG TGGTCGATAT ATCTCTGCAG CACCTGGAGC AGAAGCAAAA
    TACCGAAGTG CTTCAGGCAC TTCCAGTCTA TTTAGCTCCA GCAGCCAGCT CTTTCCTCCT
    TCTCGGCTTC GGTATAATAG GTCTGATATT ATGCCTTCTG GCCGAAGTAG ATTATTAGAA
    GATTTCAGAA ACAACCGCTT CCCAAACCTT CAACTTAGAG ACTTGATTGG ACACATAGTT
    GAGTTTTCTC AAGACCAGCA TGGTTCTAGA TTCATACAGC AAAAGCTAGA GAGAGCTACT
    CCAGCTGAGC GACAGATGGT TTTTAATGAA ATTCTACAAG CAGCCTATCA ATTAATGACA
    GATGTTTTTG GCAACTATGT CATACAGAAA TTTTTTGAGT TTGGGAGTTT GGATCAAAAA
    TTAGCCCTAG CTACTCGTAT TCGAGGTCAT GTTCTACCCT TAGCATTGCA GATGTATGGC
    TGCCGTGTTA TTCAGAAAGC ATTAGAGTCT ATTTCATCTG ACCAGCAGGT AATTAGTGAA
    ATGGTAAAGG AGCTCGATGG TCATGTACTC AAATGTGTGA AAGATCAAAA TGGAAACCAT
    GTTGTACAAA AGTGTATTGA ATGTGTTCAG CCACAGTCAC TACAGTTCAT CATTGATGCT
    TTCAAAGGAC AAGTATTTGT ACTTTCAACT CATCCTTATG GCTGCAGAGT AATCCAGCGC
    ATTCTGGAGC ATTGCACTGC AGAGCAGACC TTACCTATCT TAGAAGAACT CCACCAACAT
    ACGGAGCAGT TGGTACAGGA TCAGTATGGC AATTACGTTA TTCAGCATGT ACTGGAACAT
    GGTCGACCTG AAGACAAGAG CAAAATTGTT TCTGAAATCC GAGGAAAGGT CTTAGCCTTG
    AGTCAACACA AATTTGCCAG CAATGTAGTA GAAAAGTGTG TTACTCATGC CTCCCGTGCT
    GAGAGAGCTT TACTGATTGA CGAAGTTTGC TGCCAGAATG ATGGTCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAT TATGTCGTTC AGAAGATGAT TGATATGGCT
    GAACCTGCTC AGAGAAAGAT AATCATGCAC AAGATTCGAC CTCACATTTC TACTTTACGC
    AAATATACAT ATGGGAAGCA TATACTGGCC AAGTTGGAAA AATATTATTT GAAAAATAGC
    CCAGATCTAG GGCCTATTGG AGGACCACCA AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06995
    Clone ID Related Accession (Same CDS sequence) XM_005193973.3 , XM_005213100.3 , XM_005213100.4
    Accession Version XM_005213100.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213100.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG CTATCAAGTA
    CTAGCTCCAA CTGCCTATTA TGATCAGACC GGTGCCTTAG TGGTTGGCCC TGGGGCAAGA
    ACTGGCCTTG GAGCTCCAGT TCGATTAATG GCTCCAACAC CTGTTTTAAT TAGTTCAGCA
    GCAGCACAAG CTGCAGCGGC AGCAGCAGCT GGAGGAACTG CAAATAGTCT CACAGGCAGC
    ACAAATGGTC TGTTCCGGCC AATTGGCACT CAGCCACCAC CGCAGCAGCA GCAGCAGCAG
    CAGCCAAGCA CTAATCTGCA GTCTAATTCA TTTTATGGGA GCACTTCTCT GACTAATAGC
    TCCCAGAGCA GTTCTTTATT TTCTCACGGA CCTGGCCAGC CTGGAAGTAC ATCTCTTGGC
    TTTGGAAGCA GTAGCTCTTT GGGTGCTGCT ATAGGCTCAG CCCTCAGTGG ATTTGGTTCA
    TCAGTTGGCA GTTCTGCAAG TAGTAGTGCC ACAAGGAGAG AGTCTCTATC TACTAGCTCT
    GACTTGTACA AAAGATCTAG TAGCAGCCTA GCACCCATAG GGCAACCATT TTACAATAGT
    CTGGGATTTT CCTCCTCTCC AAGTCCAATA GGCATGCCTC TGCCAAGCCA AACTCCAGGA
    CATTCACTTA CGCCACCGCC ATCACTTTCA TCACATGGAT CCTCATCCAG TTTGCATTTA
    GGAGGACTGA CAAATGGTAG TGGTCGATAT ATCTCTGCAG CACCTGGAGC AGAAGCAAAA
    TACCGAAGTG CTTCAGGCAC TTCCAGTCTA TTTAGCTCCA GCAGCCAGCT CTTTCCTCCT
    TCTCGGCTTC GGTATAATAG GTCTGATATT ATGCCTTCTG GCCGAAGTAG ATTATTAGAA
    GATTTCAGAA ACAACCGCTT CCCAAACCTT CAACTTAGAG ACTTGATTGG ACACATAGTT
    GAGTTTTCTC AAGACCAGCA TGGTTCTAGA TTCATACAGC AAAAGCTAGA GAGAGCTACT
    CCAGCTGAGC GACAGATGGT TTTTAATGAA ATTCTACAAG CAGCCTATCA ATTAATGACA
    GATGTTTTTG GCAACTATGT CATACAGAAA TTTTTTGAGT TTGGGAGTTT GGATCAAAAA
    TTAGCCCTAG CTACTCGTAT TCGAGGTCAT GTTCTACCCT TAGCATTGCA GATGTATGGC
    TGCCGTGTTA TTCAGAAAGC ATTAGAGTCT ATTTCATCTG ACCAGCAGGT AATTAGTGAA
    ATGGTAAAGG AGCTCGATGG TCATGTACTC AAATGTGTGA AAGATCAAAA TGGAAACCAT
    GTTGTACAAA AGTGTATTGA ATGTGTTCAG CCACAGTCAC TACAGTTCAT CATTGATGCT
    TTCAAAGGAC AAGTATTTGT ACTTTCAACT CATCCTTATG GCTGCAGAGT AATCCAGCGC
    ATTCTGGAGC ATTGCACTGC AGAGCAGACC TTACCTATCT TAGAAGAACT CCACCAACAT
    ACGGAGCAGT TGGTACAGGA TCAGTATGGC AATTACGTTA TTCAGCATGT ACTGGAACAT
    GGTCGACCTG AAGACAAGAG CAAAATTGTT TCTGAAATCC GAGGAAAGGT CTTAGCCTTG
    AGTCAACACA AATTTGCCAG CAATGTAGTA GAAAAGTGTG TTACTCATGC CTCCCGTGCT
    GAGAGAGCTT TACTGATTGA CGAAGTTTGC TGCCAGAATG ATGGTCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAT TATGTCGTTC AGAAGATGAT TGATATGGCT
    GAACCTGCTC AGAGAAAGAT AATCATGCAC AAGATTCGAC CTCACATTTC TACTTTACGC
    AAATATACAT ATGGGAAGCA TATACTGGCC AAGTTGGAAA AATATTATTT GAAAAATAGC
    CCAGATCTAG GGCCTATTGG AGGACCACCA AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213157.1
    CDS167..3091
    Translation

    Target ORF information:

    RefSeq Version XM_005213100.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213100.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG CTATCAAGTA
    CTAGCTCCAA CTGCCTATTA TGATCAGACC GGTGCCTTAG TGGTTGGCCC TGGGGCAAGA
    ACTGGCCTTG GAGCTCCAGT TCGATTAATG GCTCCAACAC CTGTTTTAAT TAGTTCAGCA
    GCAGCACAAG CTGCAGCGGC AGCAGCAGCT GGAGGAACTG CAAATAGTCT CACAGGCAGC
    ACAAATGGTC TGTTCCGGCC AATTGGCACT CAGCCACCAC CGCAGCAGCA GCAGCAGCAG
    CAGCCAAGCA CTAATCTGCA GTCTAATTCA TTTTATGGGA GCACTTCTCT GACTAATAGC
    TCCCAGAGCA GTTCTTTATT TTCTCACGGA CCTGGCCAGC CTGGAAGTAC ATCTCTTGGC
    TTTGGAAGCA GTAGCTCTTT GGGTGCTGCT ATAGGCTCAG CCCTCAGTGG ATTTGGTTCA
    TCAGTTGGCA GTTCTGCAAG TAGTAGTGCC ACAAGGAGAG AGTCTCTATC TACTAGCTCT
    GACTTGTACA AAAGATCTAG TAGCAGCCTA GCACCCATAG GGCAACCATT TTACAATAGT
    CTGGGATTTT CCTCCTCTCC AAGTCCAATA GGCATGCCTC TGCCAAGCCA AACTCCAGGA
    CATTCACTTA CGCCACCGCC ATCACTTTCA TCACATGGAT CCTCATCCAG TTTGCATTTA
    GGAGGACTGA CAAATGGTAG TGGTCGATAT ATCTCTGCAG CACCTGGAGC AGAAGCAAAA
    TACCGAAGTG CTTCAGGCAC TTCCAGTCTA TTTAGCTCCA GCAGCCAGCT CTTTCCTCCT
    TCTCGGCTTC GGTATAATAG GTCTGATATT ATGCCTTCTG GCCGAAGTAG ATTATTAGAA
    GATTTCAGAA ACAACCGCTT CCCAAACCTT CAACTTAGAG ACTTGATTGG ACACATAGTT
    GAGTTTTCTC AAGACCAGCA TGGTTCTAGA TTCATACAGC AAAAGCTAGA GAGAGCTACT
    CCAGCTGAGC GACAGATGGT TTTTAATGAA ATTCTACAAG CAGCCTATCA ATTAATGACA
    GATGTTTTTG GCAACTATGT CATACAGAAA TTTTTTGAGT TTGGGAGTTT GGATCAAAAA
    TTAGCCCTAG CTACTCGTAT TCGAGGTCAT GTTCTACCCT TAGCATTGCA GATGTATGGC
    TGCCGTGTTA TTCAGAAAGC ATTAGAGTCT ATTTCATCTG ACCAGCAGGT AATTAGTGAA
    ATGGTAAAGG AGCTCGATGG TCATGTACTC AAATGTGTGA AAGATCAAAA TGGAAACCAT
    GTTGTACAAA AGTGTATTGA ATGTGTTCAG CCACAGTCAC TACAGTTCAT CATTGATGCT
    TTCAAAGGAC AAGTATTTGT ACTTTCAACT CATCCTTATG GCTGCAGAGT AATCCAGCGC
    ATTCTGGAGC ATTGCACTGC AGAGCAGACC TTACCTATCT TAGAAGAACT CCACCAACAT
    ACGGAGCAGT TGGTACAGGA TCAGTATGGC AATTACGTTA TTCAGCATGT ACTGGAACAT
    GGTCGACCTG AAGACAAGAG CAAAATTGTT TCTGAAATCC GAGGAAAGGT CTTAGCCTTG
    AGTCAACACA AATTTGCCAG CAATGTAGTA GAAAAGTGTG TTACTCATGC CTCCCGTGCT
    GAGAGAGCTT TACTGATTGA CGAAGTTTGC TGCCAGAATG ATGGTCCTCA CAGTGCCTTA
    TACACCATGA TGAAGGACCA GTATGCCAAT TATGTCGTTC AGAAGATGAT TGATATGGCT
    GAACCTGCTC AGAGAAAGAT AATCATGCAC AAGATTCGAC CTCACATTTC TACTTTACGC
    AAATATACAT ATGGGAAGCA TATACTGGCC AAGTTGGAAA AATATTATTT GAAAAATAGC
    CCAGATCTAG GGCCTATTGG AGGACCACCA AATGGAATGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54578
    Clone ID Related Accession (Same CDS sequence) XM_010810300.1 , XM_010810300.2 , XM_010817483.1
    Accession Version XM_010810300.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAACAAG TTGGTCTGGA TTCCTTACAG TTTGACTATC CTGGTAATCA GGTACCAATG 
    GACTCTTCAG GAGCTACTGT AGGCCTTTTT GACTACAATT CCCAGCAGCA GCTTTTTCAG
    AGGACTAATG CACTAACAGT TCAGCAGTTA ACTGCAGCTC AGCAGCAGCA GTATGCATTA
    GCCACAGCTC AGCAGCCACA TATAGCTGGT GTATTTTCAG CAGGCCTTGC TCCAGCTGCA
    TTTGTGCCAA ATCCATATAT TATTAGTGCT GCTCCTCCAG GGACTGATCC GTATACTGCA
    GCAGGATTGG CTGCAGCAGC TACATTAGCA GGTCCGGCAG TGGTTCCACC TCAGTATTAT
    GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT TTATTTCAGC AGCAAGCTGC AGCTGCGGCA
    AACAACACCG CAAATCAACA AGCAGCATCA CAAGCTCAGC CTGGACAGCA ACAGGTTCTC
    CGTGCTGGGG CAGGTCAGCG CCCTCTTACT CCCAATCAGG GCCAGCAAGG GCAGCAAGCT
    GAATCACTTG CAGCAGCAAA TCCAACTTTG GCTTTTGGTC AGGGTCTTGC TACCGGCATG
    CCAGGCTATC AAGTACTAGC TCCAACTGCC TATTATGATC AGACCGGTGC CTTAGTGGTT
    GGCCCTGGGG CAAGAACTGG CCTTGGAGCT CCAGTTCGAT TAATGGCTCC AACACCTGTT
    TTAATTAGTT CAGCAGCAGC ACAAGCTGCA GCGGCAGCAG CAGCTGGAGG AACTGCAAAT
    AGTCTCACAG GCAGCACAAA TGGTCTGTTC CGGCCAATTG GCACTCAGCC ACCACCGCAG
    CAGCAGCAGC AGCAGCAGCC AAGCACTAAT CTGCAGTCTA ATTCATTTTA TGGGAGCACT
    TCTCTGACTA ATAGCTCCCA GAGCAGTTCT TTATTTTCTC ACGGACCTGG CCAGCCTGGA
    AGTACATCTC TTGGCTTTGG AAGCAGTAGC TCTTTGGGTG CTGCTATAGG CTCAGCCCTC
    AGTGGATTTG GTTCATCAGT TGGCAGTTCT GCAAGTAGTA GTGCCACAAG GAGAGAGTCT
    CTATCTACTA GCTCTGACTT GTACAAAAGA TCTAGTAGCA GCCTAGCACC CATAGGGCAA
    CCATTTTACA ATAGTCTGGG ATTTTCCTCC TCTCCAAGTC CAATAGGCAT GCCTCTGCCA
    AGCCAAACTC CAGGACATTC ACTTACGCCA CCGCCATCAC TTTCATCACA TGGATCCTCA
    TCCAGTTTGC ATTTAGGAGG ACTGACAAAT GGTAGTGGTC GATATATCTC TGCAGCACCT
    GGAGCAGAAG CAAAATACCG AAGTGCTTCA GGCACTTCCA GTCTATTTAG CTCCAGCAGC
    CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT AATAGGTCTG ATATTATGCC TTCTGGCCGA
    AGTAGATTAT TAGAAGATTT CAGAAACAAC CGCTTCCCAA ACCTTCAACT TAGAGACTTG
    ATTGGACACA TAGTTGAGTT TTCTCAAGAC CAGCATGGTT CTAGATTCAT ACAGCAAAAG
    CTAGAGAGAG CTACTCCAGC TGAGCGACAG ATGGTTTTTA ATGAAATTCT ACAAGCAGCC
    TATCAATTAA TGACAGATGT TTTTGGCAAC TATGTCATAC AGAAATTTTT TGAGTTTGGG
    AGTTTGGATC AAAAATTAGC CCTAGCTACT CGTATTCGAG GTCATGTTCT ACCCTTAGCA
    TTGCAGATGT ATGGCTGCCG TGTTATTCAG AAAGCATTAG AGTCTATTTC ATCTGACCAG
    CAGGTAATTA GTGAAATGGT AAAGGAGCTC GATGGTCATG TACTCAAATG TGTGAAAGAT
    CAAAATGGAA ACCATGTTGT ACAAAAGTGT ATTGAATGTG TTCAGCCACA GTCACTACAG
    TTCATCATTG ATGCTTTCAA AGGACAAGTA TTTGTACTTT CAACTCATCC TTATGGCTGC
    AGAGTAATCC AGCGCATTCT GGAGCATTGC ACTGCAGAGC AGACCTTACC TATCTTAGAA
    GAACTCCACC AACATACGGA GCAGTTGGTA CAGGATCAGT ATGGCAATTA CGTTATTCAG
    CATGTACTGG AACATGGTCG ACCTGAAGAC AAGAGCAAAA TTGTTTCTGA AATCCGAGGA
    AAGGTCTTAG CCTTGAGTCA ACACAAATTT GCCAGCAATG TAGTAGAAAA GTGTGTTACT
    CATGCCTCCC GTGCTGAGAG AGCTTTACTG ATTGACGAAG TTTGCTGCCA GAATGATGGT
    CCTCACAGTG CCTTATACAC CATGATGAAG GACCAGTATG CCAATTATGT CGTTCAGAAG
    ATGATTGATA TGGCTGAACC TGCTCAGAGA AAGATAATCA TGCACAAGAT TCGACCTCAC
    ATTTCTACTT TACGCAAATA TACATATGGG AAGCATATAC TGGCCAAGTT GGAAAAATAT
    TATTTGAAAA ATAGCCCAGA TCTAGGGCCT ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808602.1
    CDS250..2769
    Translation

    Target ORF information:

    RefSeq Version XM_010810300.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810300.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAACAAG TTGGTCTGGA TTCCTTACAG TTTGACTATC CTGGTAATCA GGTACCAATG 
    GACTCTTCAG GAGCTACTGT AGGCCTTTTT GACTACAATT CCCAGCAGCA GCTTTTTCAG
    AGGACTAATG CACTAACAGT TCAGCAGTTA ACTGCAGCTC AGCAGCAGCA GTATGCATTA
    GCCACAGCTC AGCAGCCACA TATAGCTGGT GTATTTTCAG CAGGCCTTGC TCCAGCTGCA
    TTTGTGCCAA ATCCATATAT TATTAGTGCT GCTCCTCCAG GGACTGATCC GTATACTGCA
    GCAGGATTGG CTGCAGCAGC TACATTAGCA GGTCCGGCAG TGGTTCCACC TCAGTATTAT
    GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT TTATTTCAGC AGCAAGCTGC AGCTGCGGCA
    AACAACACCG CAAATCAACA AGCAGCATCA CAAGCTCAGC CTGGACAGCA ACAGGTTCTC
    CGTGCTGGGG CAGGTCAGCG CCCTCTTACT CCCAATCAGG GCCAGCAAGG GCAGCAAGCT
    GAATCACTTG CAGCAGCAAA TCCAACTTTG GCTTTTGGTC AGGGTCTTGC TACCGGCATG
    CCAGGCTATC AAGTACTAGC TCCAACTGCC TATTATGATC AGACCGGTGC CTTAGTGGTT
    GGCCCTGGGG CAAGAACTGG CCTTGGAGCT CCAGTTCGAT TAATGGCTCC AACACCTGTT
    TTAATTAGTT CAGCAGCAGC ACAAGCTGCA GCGGCAGCAG CAGCTGGAGG AACTGCAAAT
    AGTCTCACAG GCAGCACAAA TGGTCTGTTC CGGCCAATTG GCACTCAGCC ACCACCGCAG
    CAGCAGCAGC AGCAGCAGCC AAGCACTAAT CTGCAGTCTA ATTCATTTTA TGGGAGCACT
    TCTCTGACTA ATAGCTCCCA GAGCAGTTCT TTATTTTCTC ACGGACCTGG CCAGCCTGGA
    AGTACATCTC TTGGCTTTGG AAGCAGTAGC TCTTTGGGTG CTGCTATAGG CTCAGCCCTC
    AGTGGATTTG GTTCATCAGT TGGCAGTTCT GCAAGTAGTA GTGCCACAAG GAGAGAGTCT
    CTATCTACTA GCTCTGACTT GTACAAAAGA TCTAGTAGCA GCCTAGCACC CATAGGGCAA
    CCATTTTACA ATAGTCTGGG ATTTTCCTCC TCTCCAAGTC CAATAGGCAT GCCTCTGCCA
    AGCCAAACTC CAGGACATTC ACTTACGCCA CCGCCATCAC TTTCATCACA TGGATCCTCA
    TCCAGTTTGC ATTTAGGAGG ACTGACAAAT GGTAGTGGTC GATATATCTC TGCAGCACCT
    GGAGCAGAAG CAAAATACCG AAGTGCTTCA GGCACTTCCA GTCTATTTAG CTCCAGCAGC
    CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT AATAGGTCTG ATATTATGCC TTCTGGCCGA
    AGTAGATTAT TAGAAGATTT CAGAAACAAC CGCTTCCCAA ACCTTCAACT TAGAGACTTG
    ATTGGACACA TAGTTGAGTT TTCTCAAGAC CAGCATGGTT CTAGATTCAT ACAGCAAAAG
    CTAGAGAGAG CTACTCCAGC TGAGCGACAG ATGGTTTTTA ATGAAATTCT ACAAGCAGCC
    TATCAATTAA TGACAGATGT TTTTGGCAAC TATGTCATAC AGAAATTTTT TGAGTTTGGG
    AGTTTGGATC AAAAATTAGC CCTAGCTACT CGTATTCGAG GTCATGTTCT ACCCTTAGCA
    TTGCAGATGT ATGGCTGCCG TGTTATTCAG AAAGCATTAG AGTCTATTTC ATCTGACCAG
    CAGGTAATTA GTGAAATGGT AAAGGAGCTC GATGGTCATG TACTCAAATG TGTGAAAGAT
    CAAAATGGAA ACCATGTTGT ACAAAAGTGT ATTGAATGTG TTCAGCCACA GTCACTACAG
    TTCATCATTG ATGCTTTCAA AGGACAAGTA TTTGTACTTT CAACTCATCC TTATGGCTGC
    AGAGTAATCC AGCGCATTCT GGAGCATTGC ACTGCAGAGC AGACCTTACC TATCTTAGAA
    GAACTCCACC AACATACGGA GCAGTTGGTA CAGGATCAGT ATGGCAATTA CGTTATTCAG
    CATGTACTGG AACATGGTCG ACCTGAAGAC AAGAGCAAAA TTGTTTCTGA AATCCGAGGA
    AAGGTCTTAG CCTTGAGTCA ACACAAATTT GCCAGCAATG TAGTAGAAAA GTGTGTTACT
    CATGCCTCCC GTGCTGAGAG AGCTTTACTG ATTGACGAAG TTTGCTGCCA GAATGATGGT
    CCTCACAGTG CCTTATACAC CATGATGAAG GACCAGTATG CCAATTATGT CGTTCAGAAG
    ATGATTGATA TGGCTGAACC TGCTCAGAGA AAGATAATCA TGCACAAGAT TCGACCTCAC
    ATTTCTACTT TACGCAAATA TACATATGGG AAGCATATAC TGGCCAAGTT GGAAAAATAT
    TATTTGAAAA ATAGCCCAGA TCTAGGGCCT ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54578
    Clone ID Related Accession (Same CDS sequence) XM_010810300.1 , XM_010810300.2 , XM_010817483.1
    Accession Version XM_010810300.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010810300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAACAAG TTGGTCTGGA TTCCTTACAG TTTGACTATC CTGGTAATCA GGTACCAATG 
    GACTCTTCAG GAGCTACTGT AGGCCTTTTT GACTACAATT CCCAGCAGCA GCTTTTTCAG
    AGGACTAATG CACTAACAGT TCAGCAGTTA ACTGCAGCTC AGCAGCAGCA GTATGCATTA
    GCCACAGCTC AGCAGCCACA TATAGCTGGT GTATTTTCAG CAGGCCTTGC TCCAGCTGCA
    TTTGTGCCAA ATCCATATAT TATTAGTGCT GCTCCTCCAG GGACTGATCC GTATACTGCA
    GCAGGATTGG CTGCAGCAGC TACATTAGCA GGTCCGGCAG TGGTTCCACC TCAGTATTAT
    GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT TTATTTCAGC AGCAAGCTGC AGCTGCGGCA
    AACAACACCG CAAATCAACA AGCAGCATCA CAAGCTCAGC CTGGACAGCA ACAGGTTCTC
    CGTGCTGGGG CAGGTCAGCG CCCTCTTACT CCCAATCAGG GCCAGCAAGG GCAGCAAGCT
    GAATCACTTG CAGCAGCAAA TCCAACTTTG GCTTTTGGTC AGGGTCTTGC TACCGGCATG
    CCAGGCTATC AAGTACTAGC TCCAACTGCC TATTATGATC AGACCGGTGC CTTAGTGGTT
    GGCCCTGGGG CAAGAACTGG CCTTGGAGCT CCAGTTCGAT TAATGGCTCC AACACCTGTT
    TTAATTAGTT CAGCAGCAGC ACAAGCTGCA GCGGCAGCAG CAGCTGGAGG AACTGCAAAT
    AGTCTCACAG GCAGCACAAA TGGTCTGTTC CGGCCAATTG GCACTCAGCC ACCACCGCAG
    CAGCAGCAGC AGCAGCAGCC AAGCACTAAT CTGCAGTCTA ATTCATTTTA TGGGAGCACT
    TCTCTGACTA ATAGCTCCCA GAGCAGTTCT TTATTTTCTC ACGGACCTGG CCAGCCTGGA
    AGTACATCTC TTGGCTTTGG AAGCAGTAGC TCTTTGGGTG CTGCTATAGG CTCAGCCCTC
    AGTGGATTTG GTTCATCAGT TGGCAGTTCT GCAAGTAGTA GTGCCACAAG GAGAGAGTCT
    CTATCTACTA GCTCTGACTT GTACAAAAGA TCTAGTAGCA GCCTAGCACC CATAGGGCAA
    CCATTTTACA ATAGTCTGGG ATTTTCCTCC TCTCCAAGTC CAATAGGCAT GCCTCTGCCA
    AGCCAAACTC CAGGACATTC ACTTACGCCA CCGCCATCAC TTTCATCACA TGGATCCTCA
    TCCAGTTTGC ATTTAGGAGG ACTGACAAAT GGTAGTGGTC GATATATCTC TGCAGCACCT
    GGAGCAGAAG CAAAATACCG AAGTGCTTCA GGCACTTCCA GTCTATTTAG CTCCAGCAGC
    CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT AATAGGTCTG ATATTATGCC TTCTGGCCGA
    AGTAGATTAT TAGAAGATTT CAGAAACAAC CGCTTCCCAA ACCTTCAACT TAGAGACTTG
    ATTGGACACA TAGTTGAGTT TTCTCAAGAC CAGCATGGTT CTAGATTCAT ACAGCAAAAG
    CTAGAGAGAG CTACTCCAGC TGAGCGACAG ATGGTTTTTA ATGAAATTCT ACAAGCAGCC
    TATCAATTAA TGACAGATGT TTTTGGCAAC TATGTCATAC AGAAATTTTT TGAGTTTGGG
    AGTTTGGATC AAAAATTAGC CCTAGCTACT CGTATTCGAG GTCATGTTCT ACCCTTAGCA
    TTGCAGATGT ATGGCTGCCG TGTTATTCAG AAAGCATTAG AGTCTATTTC ATCTGACCAG
    CAGGTAATTA GTGAAATGGT AAAGGAGCTC GATGGTCATG TACTCAAATG TGTGAAAGAT
    CAAAATGGAA ACCATGTTGT ACAAAAGTGT ATTGAATGTG TTCAGCCACA GTCACTACAG
    TTCATCATTG ATGCTTTCAA AGGACAAGTA TTTGTACTTT CAACTCATCC TTATGGCTGC
    AGAGTAATCC AGCGCATTCT GGAGCATTGC ACTGCAGAGC AGACCTTACC TATCTTAGAA
    GAACTCCACC AACATACGGA GCAGTTGGTA CAGGATCAGT ATGGCAATTA CGTTATTCAG
    CATGTACTGG AACATGGTCG ACCTGAAGAC AAGAGCAAAA TTGTTTCTGA AATCCGAGGA
    AAGGTCTTAG CCTTGAGTCA ACACAAATTT GCCAGCAATG TAGTAGAAAA GTGTGTTACT
    CATGCCTCCC GTGCTGAGAG AGCTTTACTG ATTGACGAAG TTTGCTGCCA GAATGATGGT
    CCTCACAGTG CCTTATACAC CATGATGAAG GACCAGTATG CCAATTATGT CGTTCAGAAG
    ATGATTGATA TGGCTGAACC TGCTCAGAGA AAGATAATCA TGCACAAGAT TCGACCTCAC
    ATTTCTACTT TACGCAAATA TACATATGGG AAGCATATAC TGGCCAAGTT GGAAAAATAT
    TATTTGAAAA ATAGCCCAGA TCTAGGGCCT ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808602.1
    CDS300..2819
    Translation

    Target ORF information:

    RefSeq Version XM_010810300.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810300.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAACAAG TTGGTCTGGA TTCCTTACAG TTTGACTATC CTGGTAATCA GGTACCAATG 
    GACTCTTCAG GAGCTACTGT AGGCCTTTTT GACTACAATT CCCAGCAGCA GCTTTTTCAG
    AGGACTAATG CACTAACAGT TCAGCAGTTA ACTGCAGCTC AGCAGCAGCA GTATGCATTA
    GCCACAGCTC AGCAGCCACA TATAGCTGGT GTATTTTCAG CAGGCCTTGC TCCAGCTGCA
    TTTGTGCCAA ATCCATATAT TATTAGTGCT GCTCCTCCAG GGACTGATCC GTATACTGCA
    GCAGGATTGG CTGCAGCAGC TACATTAGCA GGTCCGGCAG TGGTTCCACC TCAGTATTAT
    GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT TTATTTCAGC AGCAAGCTGC AGCTGCGGCA
    AACAACACCG CAAATCAACA AGCAGCATCA CAAGCTCAGC CTGGACAGCA ACAGGTTCTC
    CGTGCTGGGG CAGGTCAGCG CCCTCTTACT CCCAATCAGG GCCAGCAAGG GCAGCAAGCT
    GAATCACTTG CAGCAGCAAA TCCAACTTTG GCTTTTGGTC AGGGTCTTGC TACCGGCATG
    CCAGGCTATC AAGTACTAGC TCCAACTGCC TATTATGATC AGACCGGTGC CTTAGTGGTT
    GGCCCTGGGG CAAGAACTGG CCTTGGAGCT CCAGTTCGAT TAATGGCTCC AACACCTGTT
    TTAATTAGTT CAGCAGCAGC ACAAGCTGCA GCGGCAGCAG CAGCTGGAGG AACTGCAAAT
    AGTCTCACAG GCAGCACAAA TGGTCTGTTC CGGCCAATTG GCACTCAGCC ACCACCGCAG
    CAGCAGCAGC AGCAGCAGCC AAGCACTAAT CTGCAGTCTA ATTCATTTTA TGGGAGCACT
    TCTCTGACTA ATAGCTCCCA GAGCAGTTCT TTATTTTCTC ACGGACCTGG CCAGCCTGGA
    AGTACATCTC TTGGCTTTGG AAGCAGTAGC TCTTTGGGTG CTGCTATAGG CTCAGCCCTC
    AGTGGATTTG GTTCATCAGT TGGCAGTTCT GCAAGTAGTA GTGCCACAAG GAGAGAGTCT
    CTATCTACTA GCTCTGACTT GTACAAAAGA TCTAGTAGCA GCCTAGCACC CATAGGGCAA
    CCATTTTACA ATAGTCTGGG ATTTTCCTCC TCTCCAAGTC CAATAGGCAT GCCTCTGCCA
    AGCCAAACTC CAGGACATTC ACTTACGCCA CCGCCATCAC TTTCATCACA TGGATCCTCA
    TCCAGTTTGC ATTTAGGAGG ACTGACAAAT GGTAGTGGTC GATATATCTC TGCAGCACCT
    GGAGCAGAAG CAAAATACCG AAGTGCTTCA GGCACTTCCA GTCTATTTAG CTCCAGCAGC
    CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT AATAGGTCTG ATATTATGCC TTCTGGCCGA
    AGTAGATTAT TAGAAGATTT CAGAAACAAC CGCTTCCCAA ACCTTCAACT TAGAGACTTG
    ATTGGACACA TAGTTGAGTT TTCTCAAGAC CAGCATGGTT CTAGATTCAT ACAGCAAAAG
    CTAGAGAGAG CTACTCCAGC TGAGCGACAG ATGGTTTTTA ATGAAATTCT ACAAGCAGCC
    TATCAATTAA TGACAGATGT TTTTGGCAAC TATGTCATAC AGAAATTTTT TGAGTTTGGG
    AGTTTGGATC AAAAATTAGC CCTAGCTACT CGTATTCGAG GTCATGTTCT ACCCTTAGCA
    TTGCAGATGT ATGGCTGCCG TGTTATTCAG AAAGCATTAG AGTCTATTTC ATCTGACCAG
    CAGGTAATTA GTGAAATGGT AAAGGAGCTC GATGGTCATG TACTCAAATG TGTGAAAGAT
    CAAAATGGAA ACCATGTTGT ACAAAAGTGT ATTGAATGTG TTCAGCCACA GTCACTACAG
    TTCATCATTG ATGCTTTCAA AGGACAAGTA TTTGTACTTT CAACTCATCC TTATGGCTGC
    AGAGTAATCC AGCGCATTCT GGAGCATTGC ACTGCAGAGC AGACCTTACC TATCTTAGAA
    GAACTCCACC AACATACGGA GCAGTTGGTA CAGGATCAGT ATGGCAATTA CGTTATTCAG
    CATGTACTGG AACATGGTCG ACCTGAAGAC AAGAGCAAAA TTGTTTCTGA AATCCGAGGA
    AAGGTCTTAG CCTTGAGTCA ACACAAATTT GCCAGCAATG TAGTAGAAAA GTGTGTTACT
    CATGCCTCCC GTGCTGAGAG AGCTTTACTG ATTGACGAAG TTTGCTGCCA GAATGATGGT
    CCTCACAGTG CCTTATACAC CATGATGAAG GACCAGTATG CCAATTATGT CGTTCAGAAG
    ATGATTGATA TGGCTGAACC TGCTCAGAGA AAGATAATCA TGCACAAGAT TCGACCTCAC
    ATTTCTACTT TACGCAAATA TACATATGGG AAGCATATAC TGGCCAAGTT GGAAAAATAT
    TATTTGAAAA ATAGCCCAGA TCTAGGGCCT ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54578
    Clone ID Related Accession (Same CDS sequence) XM_010810300.1 , XM_010810300.2 , XM_010817483.1
    Accession Version XM_010817483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAACAAG TTGGTCTGGA TTCCTTACAG TTTGACTATC CTGGTAATCA GGTACCAATG 
    GACTCTTCAG GAGCTACTGT AGGCCTTTTT GACTACAATT CCCAGCAGCA GCTTTTTCAG
    AGGACTAATG CACTAACAGT TCAGCAGTTA ACTGCAGCTC AGCAGCAGCA GTATGCATTA
    GCCACAGCTC AGCAGCCACA TATAGCTGGT GTATTTTCAG CAGGCCTTGC TCCAGCTGCA
    TTTGTGCCAA ATCCATATAT TATTAGTGCT GCTCCTCCAG GGACTGATCC GTATACTGCA
    GCAGGATTGG CTGCAGCAGC TACATTAGCA GGTCCGGCAG TGGTTCCACC TCAGTATTAT
    GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT TTATTTCAGC AGCAAGCTGC AGCTGCGGCA
    AACAACACCG CAAATCAACA AGCAGCATCA CAAGCTCAGC CTGGACAGCA ACAGGTTCTC
    CGTGCTGGGG CAGGTCAGCG CCCTCTTACT CCCAATCAGG GCCAGCAAGG GCAGCAAGCT
    GAATCACTTG CAGCAGCAAA TCCAACTTTG GCTTTTGGTC AGGGTCTTGC TACCGGCATG
    CCAGGCTATC AAGTACTAGC TCCAACTGCC TATTATGATC AGACCGGTGC CTTAGTGGTT
    GGCCCTGGGG CAAGAACTGG CCTTGGAGCT CCAGTTCGAT TAATGGCTCC AACACCTGTT
    TTAATTAGTT CAGCAGCAGC ACAAGCTGCA GCGGCAGCAG CAGCTGGAGG AACTGCAAAT
    AGTCTCACAG GCAGCACAAA TGGTCTGTTC CGGCCAATTG GCACTCAGCC ACCACCGCAG
    CAGCAGCAGC AGCAGCAGCC AAGCACTAAT CTGCAGTCTA ATTCATTTTA TGGGAGCACT
    TCTCTGACTA ATAGCTCCCA GAGCAGTTCT TTATTTTCTC ACGGACCTGG CCAGCCTGGA
    AGTACATCTC TTGGCTTTGG AAGCAGTAGC TCTTTGGGTG CTGCTATAGG CTCAGCCCTC
    AGTGGATTTG GTTCATCAGT TGGCAGTTCT GCAAGTAGTA GTGCCACAAG GAGAGAGTCT
    CTATCTACTA GCTCTGACTT GTACAAAAGA TCTAGTAGCA GCCTAGCACC CATAGGGCAA
    CCATTTTACA ATAGTCTGGG ATTTTCCTCC TCTCCAAGTC CAATAGGCAT GCCTCTGCCA
    AGCCAAACTC CAGGACATTC ACTTACGCCA CCGCCATCAC TTTCATCACA TGGATCCTCA
    TCCAGTTTGC ATTTAGGAGG ACTGACAAAT GGTAGTGGTC GATATATCTC TGCAGCACCT
    GGAGCAGAAG CAAAATACCG AAGTGCTTCA GGCACTTCCA GTCTATTTAG CTCCAGCAGC
    CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT AATAGGTCTG ATATTATGCC TTCTGGCCGA
    AGTAGATTAT TAGAAGATTT CAGAAACAAC CGCTTCCCAA ACCTTCAACT TAGAGACTTG
    ATTGGACACA TAGTTGAGTT TTCTCAAGAC CAGCATGGTT CTAGATTCAT ACAGCAAAAG
    CTAGAGAGAG CTACTCCAGC TGAGCGACAG ATGGTTTTTA ATGAAATTCT ACAAGCAGCC
    TATCAATTAA TGACAGATGT TTTTGGCAAC TATGTCATAC AGAAATTTTT TGAGTTTGGG
    AGTTTGGATC AAAAATTAGC CCTAGCTACT CGTATTCGAG GTCATGTTCT ACCCTTAGCA
    TTGCAGATGT ATGGCTGCCG TGTTATTCAG AAAGCATTAG AGTCTATTTC ATCTGACCAG
    CAGGTAATTA GTGAAATGGT AAAGGAGCTC GATGGTCATG TACTCAAATG TGTGAAAGAT
    CAAAATGGAA ACCATGTTGT ACAAAAGTGT ATTGAATGTG TTCAGCCACA GTCACTACAG
    TTCATCATTG ATGCTTTCAA AGGACAAGTA TTTGTACTTT CAACTCATCC TTATGGCTGC
    AGAGTAATCC AGCGCATTCT GGAGCATTGC ACTGCAGAGC AGACCTTACC TATCTTAGAA
    GAACTCCACC AACATACGGA GCAGTTGGTA CAGGATCAGT ATGGCAATTA CGTTATTCAG
    CATGTACTGG AACATGGTCG ACCTGAAGAC AAGAGCAAAA TTGTTTCTGA AATCCGAGGA
    AAGGTCTTAG CCTTGAGTCA ACACAAATTT GCCAGCAATG TAGTAGAAAA GTGTGTTACT
    CATGCCTCCC GTGCTGAGAG AGCTTTACTG ATTGACGAAG TTTGCTGCCA GAATGATGGT
    CCTCACAGTG CCTTATACAC CATGATGAAG GACCAGTATG CCAATTATGT CGTTCAGAAG
    ATGATTGATA TGGCTGAACC TGCTCAGAGA AAGATAATCA TGCACAAGAT TCGACCTCAC
    ATTTCTACTT TACGCAAATA TACATATGGG AAGCATATAC TGGCCAAGTT GGAAAAATAT
    TATTTGAAAA ATAGCCCAGA TCTAGGGCCT ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815785.1
    CDS250..2769
    Translation

    Target ORF information:

    RefSeq Version XM_010817483.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAACAAG TTGGTCTGGA TTCCTTACAG TTTGACTATC CTGGTAATCA GGTACCAATG 
    GACTCTTCAG GAGCTACTGT AGGCCTTTTT GACTACAATT CCCAGCAGCA GCTTTTTCAG
    AGGACTAATG CACTAACAGT TCAGCAGTTA ACTGCAGCTC AGCAGCAGCA GTATGCATTA
    GCCACAGCTC AGCAGCCACA TATAGCTGGT GTATTTTCAG CAGGCCTTGC TCCAGCTGCA
    TTTGTGCCAA ATCCATATAT TATTAGTGCT GCTCCTCCAG GGACTGATCC GTATACTGCA
    GCAGGATTGG CTGCAGCAGC TACATTAGCA GGTCCGGCAG TGGTTCCACC TCAGTATTAT
    GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT TTATTTCAGC AGCAAGCTGC AGCTGCGGCA
    AACAACACCG CAAATCAACA AGCAGCATCA CAAGCTCAGC CTGGACAGCA ACAGGTTCTC
    CGTGCTGGGG CAGGTCAGCG CCCTCTTACT CCCAATCAGG GCCAGCAAGG GCAGCAAGCT
    GAATCACTTG CAGCAGCAAA TCCAACTTTG GCTTTTGGTC AGGGTCTTGC TACCGGCATG
    CCAGGCTATC AAGTACTAGC TCCAACTGCC TATTATGATC AGACCGGTGC CTTAGTGGTT
    GGCCCTGGGG CAAGAACTGG CCTTGGAGCT CCAGTTCGAT TAATGGCTCC AACACCTGTT
    TTAATTAGTT CAGCAGCAGC ACAAGCTGCA GCGGCAGCAG CAGCTGGAGG AACTGCAAAT
    AGTCTCACAG GCAGCACAAA TGGTCTGTTC CGGCCAATTG GCACTCAGCC ACCACCGCAG
    CAGCAGCAGC AGCAGCAGCC AAGCACTAAT CTGCAGTCTA ATTCATTTTA TGGGAGCACT
    TCTCTGACTA ATAGCTCCCA GAGCAGTTCT TTATTTTCTC ACGGACCTGG CCAGCCTGGA
    AGTACATCTC TTGGCTTTGG AAGCAGTAGC TCTTTGGGTG CTGCTATAGG CTCAGCCCTC
    AGTGGATTTG GTTCATCAGT TGGCAGTTCT GCAAGTAGTA GTGCCACAAG GAGAGAGTCT
    CTATCTACTA GCTCTGACTT GTACAAAAGA TCTAGTAGCA GCCTAGCACC CATAGGGCAA
    CCATTTTACA ATAGTCTGGG ATTTTCCTCC TCTCCAAGTC CAATAGGCAT GCCTCTGCCA
    AGCCAAACTC CAGGACATTC ACTTACGCCA CCGCCATCAC TTTCATCACA TGGATCCTCA
    TCCAGTTTGC ATTTAGGAGG ACTGACAAAT GGTAGTGGTC GATATATCTC TGCAGCACCT
    GGAGCAGAAG CAAAATACCG AAGTGCTTCA GGCACTTCCA GTCTATTTAG CTCCAGCAGC
    CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT AATAGGTCTG ATATTATGCC TTCTGGCCGA
    AGTAGATTAT TAGAAGATTT CAGAAACAAC CGCTTCCCAA ACCTTCAACT TAGAGACTTG
    ATTGGACACA TAGTTGAGTT TTCTCAAGAC CAGCATGGTT CTAGATTCAT ACAGCAAAAG
    CTAGAGAGAG CTACTCCAGC TGAGCGACAG ATGGTTTTTA ATGAAATTCT ACAAGCAGCC
    TATCAATTAA TGACAGATGT TTTTGGCAAC TATGTCATAC AGAAATTTTT TGAGTTTGGG
    AGTTTGGATC AAAAATTAGC CCTAGCTACT CGTATTCGAG GTCATGTTCT ACCCTTAGCA
    TTGCAGATGT ATGGCTGCCG TGTTATTCAG AAAGCATTAG AGTCTATTTC ATCTGACCAG
    CAGGTAATTA GTGAAATGGT AAAGGAGCTC GATGGTCATG TACTCAAATG TGTGAAAGAT
    CAAAATGGAA ACCATGTTGT ACAAAAGTGT ATTGAATGTG TTCAGCCACA GTCACTACAG
    TTCATCATTG ATGCTTTCAA AGGACAAGTA TTTGTACTTT CAACTCATCC TTATGGCTGC
    AGAGTAATCC AGCGCATTCT GGAGCATTGC ACTGCAGAGC AGACCTTACC TATCTTAGAA
    GAACTCCACC AACATACGGA GCAGTTGGTA CAGGATCAGT ATGGCAATTA CGTTATTCAG
    CATGTACTGG AACATGGTCG ACCTGAAGAC AAGAGCAAAA TTGTTTCTGA AATCCGAGGA
    AAGGTCTTAG CCTTGAGTCA ACACAAATTT GCCAGCAATG TAGTAGAAAA GTGTGTTACT
    CATGCCTCCC GTGCTGAGAG AGCTTTACTG ATTGACGAAG TTTGCTGCCA GAATGATGGT
    CCTCACAGTG CCTTATACAC CATGATGAAG GACCAGTATG CCAATTATGT CGTTCAGAAG
    ATGATTGATA TGGCTGAACC TGCTCAGAGA AAGATAATCA TGCACAAGAT TCGACCTCAC
    ATTTCTACTT TACGCAAATA TACATATGGG AAGCATATAC TGGCCAAGTT GGAAAAATAT
    TATTTGAAAA ATAGCCCAGA TCTAGGGCCT ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_015473570.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329056.1
    CDS38..3235
    Translation

    Target ORF information:

    RefSeq Version XM_015473570.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_005213096.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213096.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213153.1
    CDS88..3285
    Translation

    Target ORF information:

    RefSeq Version XM_005213096.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213096.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_015465434.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320920.1
    CDS38..3235
    Translation

    Target ORF information:

    RefSeq Version XM_015465434.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465434.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_015473569.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329055.1
    CDS58..3255
    Translation

    Target ORF information:

    RefSeq Version XM_015473569.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473569.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_005213096.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213153.1
    CDS83..3280
    Translation

    Target ORF information:

    RefSeq Version XM_005213096.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_015473569.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329055.1
    CDS38..3235
    Translation

    Target ORF information:

    RefSeq Version XM_015473569.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_005193969.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193969.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194026.1
    CDS83..3280
    Translation

    Target ORF information:

    RefSeq Version XM_005193969.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193969.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_005213092.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213092.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213149.1
    CDS167..3364
    Translation

    Target ORF information:

    RefSeq Version XM_005213092.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213092.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_005213092.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213092.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213149.1
    CDS150..3347
    Translation

    Target ORF information:

    RefSeq Version XM_005213092.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213092.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_005193965.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193965.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194022.1
    CDS150..3347
    Translation

    Target ORF information:

    RefSeq Version XM_005193965.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193965.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_015465435.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320921.1
    CDS38..3235
    Translation

    Target ORF information:

    RefSeq Version XM_015465435.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465435.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_015473570.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329056.1
    CDS42..3239
    Translation

    Target ORF information:

    RefSeq Version XM_015473570.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473570.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06990
    Clone ID Related Accession (Same CDS sequence) XM_015473570.1 , XM_005213096.4 , XM_015465434.1 , XM_015473569.2 , XM_005213096.3 , XM_015473569.1 , XM_005193969.3 , XM_005213092.4 , XM_005213092.3 , XM_005193965.3 , XM_015465435.1 , XM_015473570.2 , XM_024999522.1
    Accession Version XM_024999522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855290.1
    CDS38..3235
    Translation

    Target ORF information:

    RefSeq Version XM_024999522.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GGTAATTAGT GAAATGGTAA AGGAGCTCGA TGGTCATGTA
    CTCAAATGTG TGAAAGATCA AAATGGAAAC CATGTTGTAC AAAAGTGTAT TGAATGTGTT
    CAGCCACAGT CACTACAGTT CATCATTGAT GCTTTCAAAG GACAAGTATT TGTACTTTCA
    ACTCATCCTT ATGGCTGCAG AGTAATCCAG CGCATTCTGG AGCATTGCAC TGCAGAGCAG
    ACCTTACCTA TCTTAGAAGA ACTCCACCAA CATACGGAGC AGTTGGTACA GGATCAGTAT
    GGCAATTACG TTATTCAGCA TGTACTGGAA CATGGTCGAC CTGAAGACAA GAGCAAAATT
    GTTTCTGAAA TCCGAGGAAA GGTCTTAGCC TTGAGTCAAC ACAAATTTGC CAGCAATGTA
    GTAGAAAAGT GTGTTACTCA TGCCTCCCGT GCTGAGAGAG CTTTACTGAT TGACGAAGTT
    TGCTGCCAGA ATGATGGTCC TCACAGTGCC TTATACACCA TGATGAAGGA CCAGTATGCC
    AATTATGTCG TTCAGAAGAT GATTGATATG GCTGAACCTG CTCAGAGAAA GATAATCATG
    CACAAGATTC GACCTCACAT TTCTACTTTA CGCAAATATA CATATGGGAA GCATATACTG
    GCCAAGTTGG AAAAATATTA TTTGAAAAAT AGCCCAGATC TAGGGCCTAT TGGAGGACCA
    CCAAATGGAA TGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06991
    Clone ID Related Accession (Same CDS sequence) XM_024999523.1 , XM_024999524.1 , XM_005193970.3 , XM_005213097.3 , XM_005213097.4
    Accession Version XM_024999523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855291.1
    CDS59..3250
    Translation

    Target ORF information:

    RefSeq Version XM_024999523.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06991
    Clone ID Related Accession (Same CDS sequence) XM_024999523.1 , XM_024999524.1 , XM_005193970.3 , XM_005213097.3 , XM_005213097.4
    Accession Version XM_024999524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855292.1
    CDS47..3238
    Translation

    Target ORF information:

    RefSeq Version XM_024999524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06991
    Clone ID Related Accession (Same CDS sequence) XM_024999523.1 , XM_024999524.1 , XM_005193970.3 , XM_005213097.3 , XM_005213097.4
    Accession Version XM_005193970.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005193970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194027.1
    CDS150..3341
    Translation

    Target ORF information:

    RefSeq Version XM_005193970.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005193970.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06991
    Clone ID Related Accession (Same CDS sequence) XM_024999523.1 , XM_024999524.1 , XM_005193970.3 , XM_005213097.3 , XM_005213097.4
    Accession Version XM_005213097.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213154.1
    CDS150..3341
    Translation

    Target ORF information:

    RefSeq Version XM_005213097.3
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213097.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06991
    Clone ID Related Accession (Same CDS sequence) XM_024999523.1 , XM_024999524.1 , XM_005193970.3 , XM_005213097.3 , XM_005213097.4
    Accession Version XM_005213097.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005213097.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213154.1
    CDS272..3463
    Translation

    Target ORF information:

    RefSeq Version XM_005213097.4
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213097.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAATCATG ATTTTCAAGC TCTTGCATTA GAATCTCGGG GAATGGGAGA GCTTTTGCCT 
    ACCAAAAAAT TTTGGGAACC TGATGATTCA ACAAAAGATG GACAAAAAGG CATATTTCTT
    GGGGATGATG AATGGAGAGA GACTGCATGG GGAACTTCTC ACCATTCAAT GTCCCAGCCT
    ATTATGGTAC AGAGAAGATC TGGACAGGGT TTTCATGGAA ACAGTGAAGT AAATGCAATA
    TTGTCTCCGC GATCAGAAAG TGGAGGCCTT GGTGTGAGCA TGGTAGAATA TGTATTAAGT
    TCTTCTCCTG CTGATAAATT GGATTCTCGA TTTAGGAAGG GAACTTTTGG CACTAGAGAT
    GCTGAAACAG ATGGACCTGA GAAAGGAGAT CAAAAAGGCA AGGCTTCTCC ATTTGAGGAG
    GACCAAAACA GAGATCTTAA GCAAGGAGAT GATGATGATT CTAAAATAAA TGGCAGAGGT
    TTGCCAAATG GAATGGATGC CGATTGCAAA GATTTTAATC GTACTCCTGG AAGTCGTCAA
    GCCTCTCCAA CTGAAGTAGT TGAGCGCCTG GGCCCCAATA CTAATCCCCC AGAAGGACTG
    GGGCCTCTTC CTAATCCTAC ATCTAATAAA CCACTTGTTG AAGAATTTTC AAATCCTGAA
    ACTCAGAATC TGGATGCCAT GGAACAAGTT GGTCTGGATT CCTTACAGTT TGACTATCCT
    GGTAATCAGG TACCAATGGA CTCTTCAGGA GCTACTGTAG GCCTTTTTGA CTACAATTCC
    CAGCAGCAGC TTTTTCAGAG GACTAATGCA CTAACAGTTC AGCAGTTAAC TGCAGCTCAG
    CAGCAGCAGT ATGCATTAGC CACAGCTCAG CAGCCACATA TAGCTGGTGT ATTTTCAGCA
    GGCCTTGCTC CAGCTGCATT TGTGCCAAAT CCATATATTA TTAGTGCTGC TCCTCCAGGG
    ACTGATCCGT ATACTGCAGC AGGATTGGCT GCAGCAGCTA CATTAGCAGG TCCGGCAGTG
    GTTCCACCTC AGTATTATGG TGTTCCGTGG GGGGTGTATC CAGCCAATTT ATTTCAGCAG
    CAAGCTGCAG CTGCGGCAAA CAACACCGCA AATCAACAAG CAGCATCACA AGCTCAGCCT
    GGACAGCAAC AGGTTCTCCG TGCTGGGGCA GGTCAGCGCC CTCTTACTCC CAATCAGGGC
    CAGCAAGGGC AGCAAGCTGA ATCACTTGCA GCAGCAAATC CAACTTTGGC TTTTGGTCAG
    GGTCTTGCTA CCGGCATGCC AGGCTATCAA GTACTAGCTC CAACTGCCTA TTATGATCAG
    ACCGGTGCCT TAGTGGTTGG CCCTGGGGCA AGAACTGGCC TTGGAGCTCC AGTTCGATTA
    ATGGCTCCAA CACCTGTTTT AATTAGTTCA GCAGCAGCAC AAGCTGCAGC GGCAGCAGCA
    GCTGGAGGAA CTGCAAATAG TCTCACAGGC AGCACAAATG GTCTGTTCCG GCCAATTGGC
    ACTCAGCCAC CACCGCAGCA GCAGCAGCAG CAGCAGCCAA GCACTAATCT GCAGTCTAAT
    TCATTTTATG GGAGCACTTC TCTGACTAAT AGCTCCCAGA GCAGTTCTTT ATTTTCTCAC
    GGACCTGGCC AGCCTGGAAG TACATCTCTT GGCTTTGGAA GCAGTAGCTC TTTGGGTGCT
    GCTATAGGCT CAGCCCTCAG TGGATTTGGT TCATCAGTTG GCAGTTCTGC AAGTAGTAGT
    GCCACAAGGA GAGAGTCTCT ATCTACTAGC TCTGACTTGT ACAAAAGATC TAGTAGCAGC
    CTAGCACCCA TAGGGCAACC ATTTTACAAT AGTCTGGGAT TTTCCTCCTC TCCAAGTCCA
    ATAGGCATGC CTCTGCCAAG CCAAACTCCA GGACATTCAC TTACGCCACC GCCATCACTT
    TCATCACATG GATCCTCATC CAGTTTGCAT TTAGGAGGAC TGACAAATGG TAGTGGTCGA
    TATATCTCTG CAGCACCTGG AGCAGAAGCA AAATACCGAA GTGCTTCAGG CACTTCCAGT
    CTATTTAGCT CCAGCAGCCA GCTCTTTCCT CCTTCTCGGC TTCGGTATAA TAGGTCTGAT
    ATTATGCCTT CTGGCCGAAG TAGATTATTA GAAGATTTCA GAAACAACCG CTTCCCAAAC
    CTTCAACTTA GAGACTTGAT TGGACACATA GTTGAGTTTT CTCAAGACCA GCATGGTTCT
    AGATTCATAC AGCAAAAGCT AGAGAGAGCT ACTCCAGCTG AGCGACAGAT GGTTTTTAAT
    GAAATTCTAC AAGCAGCCTA TCAATTAATG ACAGATGTTT TTGGCAACTA TGTCATACAG
    AAATTTTTTG AGTTTGGGAG TTTGGATCAA AAATTAGCCC TAGCTACTCG TATTCGAGGT
    CATGTTCTAC CCTTAGCATT GCAGATGTAT GGCTGCCGTG TTATTCAGAA AGCATTAGAG
    TCTATTTCAT CTGACCAGCA GAGTGAAATG GTAAAGGAGC TCGATGGTCA TGTACTCAAA
    TGTGTGAAAG ATCAAAATGG AAACCATGTT GTACAAAAGT GTATTGAATG TGTTCAGCCA
    CAGTCACTAC AGTTCATCAT TGATGCTTTC AAAGGACAAG TATTTGTACT TTCAACTCAT
    CCTTATGGCT GCAGAGTAAT CCAGCGCATT CTGGAGCATT GCACTGCAGA GCAGACCTTA
    CCTATCTTAG AAGAACTCCA CCAACATACG GAGCAGTTGG TACAGGATCA GTATGGCAAT
    TACGTTATTC AGCATGTACT GGAACATGGT CGACCTGAAG ACAAGAGCAA AATTGTTTCT
    GAAATCCGAG GAAAGGTCTT AGCCTTGAGT CAACACAAAT TTGCCAGCAA TGTAGTAGAA
    AAGTGTGTTA CTCATGCCTC CCGTGCTGAG AGAGCTTTAC TGATTGACGA AGTTTGCTGC
    CAGAATGATG GTCCTCACAG TGCCTTATAC ACCATGATGA AGGACCAGTA TGCCAATTAT
    GTCGTTCAGA AGATGATTGA TATGGCTGAA CCTGCTCAGA GAAAGATAAT CATGCACAAG
    ATTCGACCTC ACATTTCTAC TTTACGCAAA TATACATATG GGAAGCATAT ACTGGCCAAG
    TTGGAAAAAT ATTATTTGAA AAATAGCCCA GATCTAGGGC CTATTGGAGG ACCACCAAAT
    GGAATGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06992
    Clone ID Related Accession (Same CDS sequence) XM_010810298.2 , XM_010810299.2 , XM_010817472.2 , XM_010817457.2
    Accession Version XM_010810298.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808600.1
    CDS182..3211
    Translation

    Target ORF information:

    RefSeq Version XM_010810298.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810298.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06992
    Clone ID Related Accession (Same CDS sequence) XM_010810298.2 , XM_010810299.2 , XM_010817472.2 , XM_010817457.2
    Accession Version XM_010810299.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010810299.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808601.1
    CDS243..3272
    Translation

    Target ORF information:

    RefSeq Version XM_010810299.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010810299.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06992
    Clone ID Related Accession (Same CDS sequence) XM_010810298.2 , XM_010810299.2 , XM_010817472.2 , XM_010817457.2
    Accession Version XM_010817472.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815774.1
    CDS243..3272
    Translation

    Target ORF information:

    RefSeq Version XM_010817472.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817472.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa06992
    Clone ID Related Accession (Same CDS sequence) XM_010810298.2 , XM_010810299.2 , XM_010817472.2 , XM_010817457.2
    Accession Version XM_010817457.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3030bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product pumilio homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010817457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815759.1
    CDS182..3211
    Translation

    Target ORF information:

    RefSeq Version XM_010817457.2
    Organism Bos taurus(cattle)
    Definition Bos taurus pumilio RNA binding family member 2 (PUM2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010817457.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCCCAGC CTATTATGGT ACAGAGAAGA TCTGGACAGG GTTTTCATGG AAACAGTGAA 
    GTAAATGCAA TATTGTCTCC GCGATCAGAA AGTGGAGGCC TTGGTGTGAG CATGGTAGAA
    TATGTATTAA GTTCTTCTCC TGCTGATAAA TTGGATTCTC GATTTAGGAA GGGAACTTTT
    GGCACTAGAG ATGCTGAAAC AGATGGACCT GAGAAAGGAG ATCAAAAAGG CAAGGCTTCT
    CCATTTGAGG AGGACCAAAA CAGAGATCTT AAGCAAGGAG ATGATGATGA TTCTAAAATA
    AATGGCAGAG GTTTGCCAAA TGGAATGGAT GCCGATTGCA AAGATTTTAA TCGTACTCCT
    GGAAGTCGTC AAGCCTCTCC AACTGAAGTA GTTGAGCGCC TGGGCCCCAA TACTAATCCC
    CCAGAAGGAC TGGGGCCTCT TCCTAATCCT ACATCTAATA AACCACTTGT TGAAGAATTT
    TCAAATCCTG AAACTCAGAA TCTGGATGCC ATGGAACAAG TTGGTCTGGA TTCCTTACAG
    TTTGACTATC CTGGTAATCA GGTACCAATG GACTCTTCAG GAGCTACTGT AGGCCTTTTT
    GACTACAATT CCCAGCAGCA GCTTTTTCAG AGGACTAATG CACTAACAGT TCAGCAGTTA
    ACTGCAGCTC AGCAGCAGCA GTATGCATTA GCCACAGCTC AGCAGCCACA TATAGCTGGT
    GTATTTTCAG CAGGCCTTGC TCCAGCTGCA TTTGTGCCAA ATCCATATAT TATTAGTGCT
    GCTCCTCCAG GGACTGATCC GTATACTGCA GCAGGATTGG CTGCAGCAGC TACATTAGCA
    GGTCCGGCAG TGGTTCCACC TCAGTATTAT GGTGTTCCGT GGGGGGTGTA TCCAGCCAAT
    TTATTTCAGC AGCAAGCTGC AGCTGCGGCA AACAACACCG CAAATCAACA AGCAGCATCA
    CAAGCTCAGC CTGGACAGCA ACAGGTTCTC CGTGCTGGGG CAGGTCAGCG CCCTCTTACT
    CCCAATCAGG GCCAGCAAGG GCAGCAAGCT GAATCACTTG CAGCAGCAAA TCCAACTTTG
    GCTTTTGGTC AGGGTCTTGC TACCGGCATG CCAGGCTATC AAGTACTAGC TCCAACTGCC
    TATTATGATC AGACCGGTGC CTTAGTGGTT GGCCCTGGGG CAAGAACTGG CCTTGGAGCT
    CCAGTTCGAT TAATGGCTCC AACACCTGTT TTAATTAGTT CAGCAGCAGC ACAAGCTGCA
    GCGGCAGCAG CAGCTGGAGG AACTGCAAAT AGTCTCACAG GCAGCACAAA TGGTCTGTTC
    CGGCCAATTG GCACTCAGCC ACCACCGCAG CAGCAGCAGC AGCAGCAGCC AAGCACTAAT
    CTGCAGTCTA ATTCATTTTA TGGGAGCACT TCTCTGACTA ATAGCTCCCA GAGCAGTTCT
    TTATTTTCTC ACGGACCTGG CCAGCCTGGA AGTACATCTC TTGGCTTTGG AAGCAGTAGC
    TCTTTGGGTG CTGCTATAGG CTCAGCCCTC AGTGGATTTG GTTCATCAGT TGGCAGTTCT
    GCAAGTAGTA GTGCCACAAG GAGAGAGTCT CTATCTACTA GCTCTGACTT GTACAAAAGA
    TCTAGTAGCA GCCTAGCACC CATAGGGCAA CCATTTTACA ATAGTCTGGG ATTTTCCTCC
    TCTCCAAGTC CAATAGGCAT GCCTCTGCCA AGCCAAACTC CAGGACATTC ACTTACGCCA
    CCGCCATCAC TTTCATCACA TGGATCCTCA TCCAGTTTGC ATTTAGGAGG ACTGACAAAT
    GGTAGTGGTC GATATATCTC TGCAGCACCT GGAGCAGAAG CAAAATACCG AAGTGCTTCA
    GGCACTTCCA GTCTATTTAG CTCCAGCAGC CAGCTCTTTC CTCCTTCTCG GCTTCGGTAT
    AATAGGTCTG ATATTATGCC TTCTGGCCGA AGTAGATTAT TAGAAGATTT CAGAAACAAC
    CGCTTCCCAA ACCTTCAACT TAGAGACTTG ATTGGACACA TAGTTGAGTT TTCTCAAGAC
    CAGCATGGTT CTAGATTCAT ACAGCAAAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG
    ATGGTTTTTA ATGAAATTCT ACAAGCAGCC TATCAATTAA TGACAGATGT TTTTGGCAAC
    TATGTCATAC AGAAATTTTT TGAGTTTGGG AGTTTGGATC AAAAATTAGC CCTAGCTACT
    CGTATTCGAG GTCATGTTCT ACCCTTAGCA TTGCAGATGT ATGGCTGCCG TGTTATTCAG
    AAAGCATTAG AGTCTATTTC ATCTGACCAG CAGGTAATTA GTGAAATGGT AAAGGAGCTC
    GATGGTCATG TACTCAAATG TGTGAAAGAT CAAAATGGAA ACCATGTTGT ACAAAAGTGT
    ATTGAATGTG TTCAGCCACA GTCACTACAG TTCATCATTG ATGCTTTCAA AGGACAAGTA
    TTTGTACTTT CAACTCATCC TTATGGCTGC AGAGTAATCC AGCGCATTCT GGAGCATTGC
    ACTGCAGAGC AGACCTTACC TATCTTAGAA GAACTCCACC AACATACGGA GCAGTTGGTA
    CAGGATCAGT ATGGCAATTA CGTTATTCAG CATGTACTGG AACATGGTCG ACCTGAAGAC
    AAGAGCAAAA TTGTTTCTGA AATCCGAGGA AAGGTCTTAG CCTTGAGTCA ACACAAATTT
    GCCAGCAATG TAGTAGAAAA GTGTGTTACT CATGCCTCCC GTGCTGAGAG AGCTTTACTG
    ATTGACGAAG TTTGCTGCCA GAATGATGGT CCTCACAGTG CCTTATACAC CATGATGAAG
    GACCAGTATG CCAATTATGT CGTTCAGAAG ATGATTGATA TGGCTGAACC TGCTCAGAGA
    AAGATAATCA TGCACAAGAT TCGACCTCAC ATTTCTACTT TACGCAAATA TACATATGGG
    AAGCATATAC TGGCCAAGTT GGAAAAATAT TATTTGAAAA ATAGCCCAGA TCTAGGGCCT
    ATTGGAGGAC CACCAAATGG AATGCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.