KDM2A cDNA ORF clone, Bos taurus(cattle)

The following KDM2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KDM2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa232233 XM_024987460.1
Latest version!
Bos taurus lysine demethylase 2A (KDM2A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa232230 XM_024987456.1
Latest version!
Bos taurus lysine demethylase 2A (KDM2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa232230 XM_024987455.1
Latest version!
Bos taurus lysine demethylase 2A (KDM2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa232231 XM_024987457.1
Latest version!
Bos taurus lysine demethylase 2A (KDM2A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa232232 XM_024987458.1
Latest version!
Bos taurus lysine demethylase 2A (KDM2A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa89123 XM_015461297.1
Latest version!
Bos taurus lysine (K)-specific demethylase 2A (KDM2A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa95290 XM_015470056.1
Latest version!
Bos taurus lysine (K)-specific demethylase 2A (KDM2A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa232233
    Clone ID Related Accession (Same CDS sequence) XM_024987460.1
    Accession Version XM_024987460.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCTGTTTG ATCAAGTGGA GGACAGAAAA GCCCACAACC TGGAGCTGTA CGAGAATTGG 
    CTGCTGTCAG GGAAACAGGG AGACATCTTT CTGGGTGACC GGGTATCAGA TTGCCAACGC
    ATTGAGCTCA AGCAGGGCTA TACCTTCGTC ATTCCCTCAG GCTGGATTCA TGCTGTGTAT
    ACTCCTACAG ACACCTTGGT GTTTGGAGGC AATTTTTTGC ATAGCTTCAA TATCCCCATG
    CAGTTAAAGA TATACAACAT TGAAGATCGA ACACGGGTTC CTAATAAGTT CCGCTATCCA
    TTCTACTACG AGATGTGCTG GTACGTGCTG GAGCGCTATG TGTACTGCAT AACCAACCGC
    TCTCACCTAA CTAAGGAATT TCAGAAAGAG TCTCTTAGCA TGGATTTGGA GTTAAATGGG
    TTGGAGTCTG GAAACGGGGA CGAGGAAGCA GTAGACCGAG AGCCCCGGCG CTTGAGCAGT
    AGACGTTCTG TCCTCACCAG CCCTGTGGCC AATGGGGTCA ACCTGGATTA TGATGGACTG
    GGCAAAACCT GCAGAAGTCT TCCAAGTCTG AAGAAAACTT TGTCTGGGGA CTCATCCTCT
    GACTCTAGCC GGGGTTCCCA GAATGGCCAA GTGTGGGATT CCCAGTGTAG CCCCCGAAAG
    GACCGACAGG TGCATCTGAC CCATTTTGAG CTTGAAGGCC TTCGTTGCCT TGTAGATAAA
    TTGGAATCTC TGCCACTGCA CAAGAAATGC GTCCCTACAG GGATAGAAGA CGAAGATGCT
    CTCATTGCTG ATGTGAAGGT TTTGCTGGAG GAACTTGCCA ACAGTGATCC CAAGTTAGCC
    CTCACTGGAG TCCCTATAGT ACAGTGGCCG AAAAGGGATA AGCTTAAATT CCCAACCCGG
    CCAAAGGTGC GGGTTCCTAC TATCCCCATC ACAAAGCCTC ACACTATGAA ACCAGCGCCG
    AGGTTAACAC CTGTGCGCCC AGCCGCTGCT TCTCCCATCG TGTCTGGAGC CAGACGGAGA
    CGAGTGCGAT GTCGAAAATG CAAAGCCTGC GTGCAGGGAG AGTGTGGTGT TTGTCACTAC
    TGCAGGGACA TGAAGAAGTT TGGGGGGCCT GGTCGCATGA AGCAGTCCTG TGTTCTCCGG
    CAGTGCTTGG CACCCAGACT GCCTCACTCT GTCACATGTT CCCTCTGTGG AGAGGTGGAT
    CAAAATGAGG AGACACAGGA CTTTGAGAAG AAACTCATGG AATGCTGTAT CTGCAATGAG
    ATTGTTCATC CTGGCTGCCT CCAGATGGAT GGAGAGGGGT TGCTTAATGA GGAATTGCCA
    AATTGCTGGG AGTGTCCAAA GTGCTACCAA GAGGACAGCT CAGAAAAAGC CCAGTGTTCT
    TCTACACCCT GGGGCCCTTG GAAAAGAGGA GCCACAGCCA GGCTGTTTGA GGAGGGAAAT
    GAGAAGCGGA AAATGGAAGA GAGTGACGAA GAAGCCGTGC AAGCCAAAGT CCTCCGGCCC
    CTGCGGAGCT GCGACGAGCC CCTCACGCCC CCACCTCACT CGCCCACCTC CATGCTGCAG
    CTCATCCACG ACCCGGCCTC CCCCCGGGGC GTGGTGACTC GGTCATCCCC TGGGGCCGGC
    CCCAGCGACC ACCACAGTGC CAGCCGCGAT GAGCGCTTCA AACGGCGGCA GTTGCTGCGG
    CTGCAGGCCA CAGAGCGCAC CATGGTACGG GAAAAGGAGA ACAATCCCAG CGGCAAAAAG
    GAGCTGTCTG AAGTTGAGAA AGCCAAGATC CGGGGATCGT ACCTCACTGT CACGCTGCAG
    AGGCCCACCA AAGAGCTCCA CGGGACATCC ATTGTGCCCA AGCTGCAGGC CATCACGGCC
    TCCTCTGCCA ACCTTCGCCA TTCCCCCCGT GTGCTGGTCC AGCACTGCCC AGCCCGAACC
    CCCCAGCGCG GGGATGAGGA GGGGCTGGGG GGAGAGGAGG AGGACGAGGA GGAGGAGGAG
    GAGGAAGATG ACAGTGCAGA GGAGGGGGGT GCGGCCAGGC TGAATGGCCG GGGCAGTTGG
    GCTCAGGATG GAGACGAAAG CTGGATGCAG CGGGAGGTCT GGATGTCTGT CTTCCGCTAC
    CTCAGCCGCA GAGAACTTTG TGAATGTATG CGAGTGTGCA AGACGTGGTA TAAATGGTGC
    TGCGACAAGA GACTTTGGAC AAAAATTGAC TTGAGTAGGT GTAAGGCTAT TGTACCCCAG
    GCTCTCAGTG GCATCATCAA GAGGCAGCCA GTTAGCCTTG ACCTCAGTTG GACCAACATC
    TCCAAAAAAC AGCTGACATG GCTCGTCAAT AGGCTGCCAG GACTGAAAGA CCTCCTCCTA
    GCAGGCTGCT CCTGGTCTGC AGTCTCTGCC CTCAGCACCT CCAGCTGCCC CCTTCTCAGG
    ACCCTTGATC TTCGGTGGGC AGTAGGAATC AAGGACCCTC AAATTCGGGA CTTGCTGACT
    CCACCGGCGG ATAAACCGGG CCAGGACAAT CGCAGCAAGC TCCGGAACAT GACCGACTTC
    CGGCTGGCAG GCCTTGACAT CACAGACGCC ACGCTTCGTC TCATCATTCG CCACATGCCC
    CTCCTGTCTC GACTCGACCT CAGTCACTGC AGCCACCTGA CAGACCAGTC CTCCAATCTG
    CTCACTGCTG TTGGGTCTTC CACTCGTTAC TCCCTCACAG AGCTCAACAT GGCAGGTTGC
    AATAAACTGA CAGACCAGAC CCTGATCTAC CTACGGCGCA TCGCCAACGT CACCTTGATC
    GACCTTCGAG GATGCAAGCA GATCACTCGA AAAGCCTGCG AGCACTTCAT CTCAGACTTG
    TCCATCAACA GTCTGTACTG CCTGTCTGAC GAGAAGCTGA TACAGAAGAT CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843228.1
    CDS142..3018
    Translation

    Target ORF information:

    RefSeq Version XM_024987460.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2A (KDM2A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987460.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCTGTTTG ATCAAGTGGA GGACAGAAAA GCCCACAACC TGGAGCTGTA CGAGAATTGG 
    CTGCTGTCAG GGAAACAGGG AGACATCTTT CTGGGTGACC GGGTATCAGA TTGCCAACGC
    ATTGAGCTCA AGCAGGGCTA TACCTTCGTC ATTCCCTCAG GCTGGATTCA TGCTGTGTAT
    ACTCCTACAG ACACCTTGGT GTTTGGAGGC AATTTTTTGC ATAGCTTCAA TATCCCCATG
    CAGTTAAAGA TATACAACAT TGAAGATCGA ACACGGGTTC CTAATAAGTT CCGCTATCCA
    TTCTACTACG AGATGTGCTG GTACGTGCTG GAGCGCTATG TGTACTGCAT AACCAACCGC
    TCTCACCTAA CTAAGGAATT TCAGAAAGAG TCTCTTAGCA TGGATTTGGA GTTAAATGGG
    TTGGAGTCTG GAAACGGGGA CGAGGAAGCA GTAGACCGAG AGCCCCGGCG CTTGAGCAGT
    AGACGTTCTG TCCTCACCAG CCCTGTGGCC AATGGGGTCA ACCTGGATTA TGATGGACTG
    GGCAAAACCT GCAGAAGTCT TCCAAGTCTG AAGAAAACTT TGTCTGGGGA CTCATCCTCT
    GACTCTAGCC GGGGTTCCCA GAATGGCCAA GTGTGGGATT CCCAGTGTAG CCCCCGAAAG
    GACCGACAGG TGCATCTGAC CCATTTTGAG CTTGAAGGCC TTCGTTGCCT TGTAGATAAA
    TTGGAATCTC TGCCACTGCA CAAGAAATGC GTCCCTACAG GGATAGAAGA CGAAGATGCT
    CTCATTGCTG ATGTGAAGGT TTTGCTGGAG GAACTTGCCA ACAGTGATCC CAAGTTAGCC
    CTCACTGGAG TCCCTATAGT ACAGTGGCCG AAAAGGGATA AGCTTAAATT CCCAACCCGG
    CCAAAGGTGC GGGTTCCTAC TATCCCCATC ACAAAGCCTC ACACTATGAA ACCAGCGCCG
    AGGTTAACAC CTGTGCGCCC AGCCGCTGCT TCTCCCATCG TGTCTGGAGC CAGACGGAGA
    CGAGTGCGAT GTCGAAAATG CAAAGCCTGC GTGCAGGGAG AGTGTGGTGT TTGTCACTAC
    TGCAGGGACA TGAAGAAGTT TGGGGGGCCT GGTCGCATGA AGCAGTCCTG TGTTCTCCGG
    CAGTGCTTGG CACCCAGACT GCCTCACTCT GTCACATGTT CCCTCTGTGG AGAGGTGGAT
    CAAAATGAGG AGACACAGGA CTTTGAGAAG AAACTCATGG AATGCTGTAT CTGCAATGAG
    ATTGTTCATC CTGGCTGCCT CCAGATGGAT GGAGAGGGGT TGCTTAATGA GGAATTGCCA
    AATTGCTGGG AGTGTCCAAA GTGCTACCAA GAGGACAGCT CAGAAAAAGC CCAGTGTTCT
    TCTACACCCT GGGGCCCTTG GAAAAGAGGA GCCACAGCCA GGCTGTTTGA GGAGGGAAAT
    GAGAAGCGGA AAATGGAAGA GAGTGACGAA GAAGCCGTGC AAGCCAAAGT CCTCCGGCCC
    CTGCGGAGCT GCGACGAGCC CCTCACGCCC CCACCTCACT CGCCCACCTC CATGCTGCAG
    CTCATCCACG ACCCGGCCTC CCCCCGGGGC GTGGTGACTC GGTCATCCCC TGGGGCCGGC
    CCCAGCGACC ACCACAGTGC CAGCCGCGAT GAGCGCTTCA AACGGCGGCA GTTGCTGCGG
    CTGCAGGCCA CAGAGCGCAC CATGGTACGG GAAAAGGAGA ACAATCCCAG CGGCAAAAAG
    GAGCTGTCTG AAGTTGAGAA AGCCAAGATC CGGGGATCGT ACCTCACTGT CACGCTGCAG
    AGGCCCACCA AAGAGCTCCA CGGGACATCC ATTGTGCCCA AGCTGCAGGC CATCACGGCC
    TCCTCTGCCA ACCTTCGCCA TTCCCCCCGT GTGCTGGTCC AGCACTGCCC AGCCCGAACC
    CCCCAGCGCG GGGATGAGGA GGGGCTGGGG GGAGAGGAGG AGGACGAGGA GGAGGAGGAG
    GAGGAAGATG ACAGTGCAGA GGAGGGGGGT GCGGCCAGGC TGAATGGCCG GGGCAGTTGG
    GCTCAGGATG GAGACGAAAG CTGGATGCAG CGGGAGGTCT GGATGTCTGT CTTCCGCTAC
    CTCAGCCGCA GAGAACTTTG TGAATGTATG CGAGTGTGCA AGACGTGGTA TAAATGGTGC
    TGCGACAAGA GACTTTGGAC AAAAATTGAC TTGAGTAGGT GTAAGGCTAT TGTACCCCAG
    GCTCTCAGTG GCATCATCAA GAGGCAGCCA GTTAGCCTTG ACCTCAGTTG GACCAACATC
    TCCAAAAAAC AGCTGACATG GCTCGTCAAT AGGCTGCCAG GACTGAAAGA CCTCCTCCTA
    GCAGGCTGCT CCTGGTCTGC AGTCTCTGCC CTCAGCACCT CCAGCTGCCC CCTTCTCAGG
    ACCCTTGATC TTCGGTGGGC AGTAGGAATC AAGGACCCTC AAATTCGGGA CTTGCTGACT
    CCACCGGCGG ATAAACCGGG CCAGGACAAT CGCAGCAAGC TCCGGAACAT GACCGACTTC
    CGGCTGGCAG GCCTTGACAT CACAGACGCC ACGCTTCGTC TCATCATTCG CCACATGCCC
    CTCCTGTCTC GACTCGACCT CAGTCACTGC AGCCACCTGA CAGACCAGTC CTCCAATCTG
    CTCACTGCTG TTGGGTCTTC CACTCGTTAC TCCCTCACAG AGCTCAACAT GGCAGGTTGC
    AATAAACTGA CAGACCAGAC CCTGATCTAC CTACGGCGCA TCGCCAACGT CACCTTGATC
    GACCTTCGAG GATGCAAGCA GATCACTCGA AAAGCCTGCG AGCACTTCAT CTCAGACTTG
    TCCATCAACA GTCTGTACTG CCTGTCTGAC GAGAAGCTGA TACAGAAGAT CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232230
    Clone ID Related Accession (Same CDS sequence) XM_024987455.1 , XM_024987456.1
    Accession Version XM_024987455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTT
    CCTAATAAGT TCCGCTATCC ATTCTACTAC GAGATGTGCT GGTACGTGCT GGAGCGCTAT
    GTGTACTGCA TAACCAACCG CTCTCACCTA ACTAAGGAAT TTCAGAAAGA GTCTCTTAGC
    ATGGATTTGG AGTTAAATGG GTTGGAGTCT GGAAACGGGG ACGAGGAAGC AGTAGACCGA
    GAGCCCCGGC GCTTGAGCAG TAGACGTTCT GTCCTCACCA GCCCTGTGGC CAATGGGGTC
    AACCTGGATT ATGATGGACT GGGCAAAACC TGCAGAAGTC TTCCAAGTCT GAAGAAAACT
    TTGTCTGGGG ACTCATCCTC TGACTCTAGC CGGGGTTCCC AGAATGGCCA AGTGTGGGAT
    TCCCAGTGTA GCCCCCGAAA GGACCGACAG GTGCATCTGA CCCATTTTGA GCTTGAAGGC
    CTTCGTTGCC TTGTAGATAA ATTGGAATCT CTGCCACTGC ACAAGAAATG CGTCCCTACA
    GGGATAGAAG ACGAAGATGC TCTCATTGCT GATGTGAAGG TTTTGCTGGA GGAACTTGCC
    AACAGTGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC GAAAAGGGAT
    AAGCTTAAAT TCCCAACCCG GCCAAAGGTG CGGGTTCCTA CTATCCCCAT CACAAAGCCT
    CACACTATGA AACCAGCGCC GAGGTTAACA CCTGTGCGCC CAGCCGCTGC TTCTCCCATC
    GTGTCTGGAG CCAGACGGAG ACGAGTGCGA TGTCGAAAAT GCAAAGCCTG CGTGCAGGGA
    GAGTGTGGTG TTTGTCACTA CTGCAGGGAC ATGAAGAAGT TTGGGGGGCC TGGTCGCATG
    AAGCAGTCCT GTGTTCTCCG GCAGTGCTTG GCACCCAGAC TGCCTCACTC TGTCACATGT
    TCCCTCTGTG GAGAGGTGGA TCAAAATGAG GAGACACAGG ACTTTGAGAA GAAACTCATG
    GAATGCTGTA TCTGCAATGA GATTGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG
    TTGCTTAATG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGCTACCA AGAGGACAGC
    TCAGAAAAAG CCCAGTGTTC TTCTACACCC TGGGGCCCTT GGAAAAGAGG AGCCACAGCC
    AGGCTGTTTG AGGAGGGAAA TGAGAAGCGG AAAATGGAAG AGAGTGACGA AGAAGCCGTG
    CAAGCCAAAG TCCTCCGGCC CCTGCGGAGC TGCGACGAGC CCCTCACGCC CCCACCTCAC
    TCGCCCACCT CCATGCTGCA GCTCATCCAC GACCCGGCCT CCCCCCGGGG CGTGGTGACT
    CGGTCATCCC CTGGGGCCGG CCCCAGCGAC CACCACAGTG CCAGCCGCGA TGAGCGCTTC
    AAACGGCGGC AGTTGCTGCG GCTGCAGGCC ACAGAGCGCA CCATGGTACG GGAAAAGGAG
    AACAATCCCA GCGGCAAAAA GGAGCTGTCT GAAGTTGAGA AAGCCAAGAT CCGGGGATCG
    TACCTCACTG TCACGCTGCA GAGGCCCACC AAAGAGCTCC ACGGGACATC CATTGTGCCC
    AAGCTGCAGG CCATCACGGC CTCCTCTGCC AACCTTCGCC ATTCCCCCCG TGTGCTGGTC
    CAGCACTGCC CAGCCCGAAC CCCCCAGCGC GGGGATGAGG AGGGGCTGGG GGGAGAGGAG
    GAGGACGAGG AGGAGGAGGA GGAGGAAGAT GACAGTGCAG AGGAGGGGGG TGCGGCCAGG
    CTGAATGGCC GGGGCAGTTG GGCTCAGGAT GGAGACGAAA GCTGGATGCA GCGGGAGGTC
    TGGATGTCTG TCTTCCGCTA CCTCAGCCGC AGAGAACTTT GTGAATGTAT GCGAGTGTGC
    AAGACGTGGT ATAAATGGTG CTGCGACAAG AGACTTTGGA CAAAAATTGA CTTGAGTAGG
    TGTAAGGCTA TTGTACCCCA GGCTCTCAGT GGCATCATCA AGAGGCAGCC AGTTAGCCTT
    GACCTCAGTT GGACCAACAT CTCCAAAAAA CAGCTGACAT GGCTCGTCAA TAGGCTGCCA
    GGACTGAAAG ACCTCCTCCT AGCAGGCTGC TCCTGGTCTG CAGTCTCTGC CCTCAGCACC
    TCCAGCTGCC CCCTTCTCAG GACCCTTGAT CTTCGGTGGG CAGTAGGAAT CAAGGACCCT
    CAAATTCGGG ACTTGCTGAC TCCACCGGCG GATAAACCGG GCCAGGACAA TCGCAGCAAG
    CTCCGGAACA TGACCGACTT CCGGCTGGCA GGCCTTGACA TCACAGACGC CACGCTTCGT
    CTCATCATTC GCCACATGCC CCTCCTGTCT CGACTCGACC TCAGTCACTG CAGCCACCTG
    ACAGACCAGT CCTCCAATCT GCTCACTGCT GTTGGGTCTT CCACTCGTTA CTCCCTCACA
    GAGCTCAACA TGGCAGGTTG CAATAAACTG ACAGACCAGA CCCTGATCTA CCTACGGCGC
    ATCGCCAACG TCACCTTGAT CGACCTTCGA GGATGCAAGC AGATCACTCG AAAAGCCTGC
    GAGCACTTCA TCTCAGACTT GTCCATCAAC AGTCTGTACT GCCTGTCTGA CGAGAAGCTG
    ATACAGAAGA TCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843223.1
    CDS498..4055
    Translation

    Target ORF information:

    RefSeq Version XM_024987455.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2A (KDM2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTT
    CCTAATAAGT TCCGCTATCC ATTCTACTAC GAGATGTGCT GGTACGTGCT GGAGCGCTAT
    GTGTACTGCA TAACCAACCG CTCTCACCTA ACTAAGGAAT TTCAGAAAGA GTCTCTTAGC
    ATGGATTTGG AGTTAAATGG GTTGGAGTCT GGAAACGGGG ACGAGGAAGC AGTAGACCGA
    GAGCCCCGGC GCTTGAGCAG TAGACGTTCT GTCCTCACCA GCCCTGTGGC CAATGGGGTC
    AACCTGGATT ATGATGGACT GGGCAAAACC TGCAGAAGTC TTCCAAGTCT GAAGAAAACT
    TTGTCTGGGG ACTCATCCTC TGACTCTAGC CGGGGTTCCC AGAATGGCCA AGTGTGGGAT
    TCCCAGTGTA GCCCCCGAAA GGACCGACAG GTGCATCTGA CCCATTTTGA GCTTGAAGGC
    CTTCGTTGCC TTGTAGATAA ATTGGAATCT CTGCCACTGC ACAAGAAATG CGTCCCTACA
    GGGATAGAAG ACGAAGATGC TCTCATTGCT GATGTGAAGG TTTTGCTGGA GGAACTTGCC
    AACAGTGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC GAAAAGGGAT
    AAGCTTAAAT TCCCAACCCG GCCAAAGGTG CGGGTTCCTA CTATCCCCAT CACAAAGCCT
    CACACTATGA AACCAGCGCC GAGGTTAACA CCTGTGCGCC CAGCCGCTGC TTCTCCCATC
    GTGTCTGGAG CCAGACGGAG ACGAGTGCGA TGTCGAAAAT GCAAAGCCTG CGTGCAGGGA
    GAGTGTGGTG TTTGTCACTA CTGCAGGGAC ATGAAGAAGT TTGGGGGGCC TGGTCGCATG
    AAGCAGTCCT GTGTTCTCCG GCAGTGCTTG GCACCCAGAC TGCCTCACTC TGTCACATGT
    TCCCTCTGTG GAGAGGTGGA TCAAAATGAG GAGACACAGG ACTTTGAGAA GAAACTCATG
    GAATGCTGTA TCTGCAATGA GATTGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG
    TTGCTTAATG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGCTACCA AGAGGACAGC
    TCAGAAAAAG CCCAGTGTTC TTCTACACCC TGGGGCCCTT GGAAAAGAGG AGCCACAGCC
    AGGCTGTTTG AGGAGGGAAA TGAGAAGCGG AAAATGGAAG AGAGTGACGA AGAAGCCGTG
    CAAGCCAAAG TCCTCCGGCC CCTGCGGAGC TGCGACGAGC CCCTCACGCC CCCACCTCAC
    TCGCCCACCT CCATGCTGCA GCTCATCCAC GACCCGGCCT CCCCCCGGGG CGTGGTGACT
    CGGTCATCCC CTGGGGCCGG CCCCAGCGAC CACCACAGTG CCAGCCGCGA TGAGCGCTTC
    AAACGGCGGC AGTTGCTGCG GCTGCAGGCC ACAGAGCGCA CCATGGTACG GGAAAAGGAG
    AACAATCCCA GCGGCAAAAA GGAGCTGTCT GAAGTTGAGA AAGCCAAGAT CCGGGGATCG
    TACCTCACTG TCACGCTGCA GAGGCCCACC AAAGAGCTCC ACGGGACATC CATTGTGCCC
    AAGCTGCAGG CCATCACGGC CTCCTCTGCC AACCTTCGCC ATTCCCCCCG TGTGCTGGTC
    CAGCACTGCC CAGCCCGAAC CCCCCAGCGC GGGGATGAGG AGGGGCTGGG GGGAGAGGAG
    GAGGACGAGG AGGAGGAGGA GGAGGAAGAT GACAGTGCAG AGGAGGGGGG TGCGGCCAGG
    CTGAATGGCC GGGGCAGTTG GGCTCAGGAT GGAGACGAAA GCTGGATGCA GCGGGAGGTC
    TGGATGTCTG TCTTCCGCTA CCTCAGCCGC AGAGAACTTT GTGAATGTAT GCGAGTGTGC
    AAGACGTGGT ATAAATGGTG CTGCGACAAG AGACTTTGGA CAAAAATTGA CTTGAGTAGG
    TGTAAGGCTA TTGTACCCCA GGCTCTCAGT GGCATCATCA AGAGGCAGCC AGTTAGCCTT
    GACCTCAGTT GGACCAACAT CTCCAAAAAA CAGCTGACAT GGCTCGTCAA TAGGCTGCCA
    GGACTGAAAG ACCTCCTCCT AGCAGGCTGC TCCTGGTCTG CAGTCTCTGC CCTCAGCACC
    TCCAGCTGCC CCCTTCTCAG GACCCTTGAT CTTCGGTGGG CAGTAGGAAT CAAGGACCCT
    CAAATTCGGG ACTTGCTGAC TCCACCGGCG GATAAACCGG GCCAGGACAA TCGCAGCAAG
    CTCCGGAACA TGACCGACTT CCGGCTGGCA GGCCTTGACA TCACAGACGC CACGCTTCGT
    CTCATCATTC GCCACATGCC CCTCCTGTCT CGACTCGACC TCAGTCACTG CAGCCACCTG
    ACAGACCAGT CCTCCAATCT GCTCACTGCT GTTGGGTCTT CCACTCGTTA CTCCCTCACA
    GAGCTCAACA TGGCAGGTTG CAATAAACTG ACAGACCAGA CCCTGATCTA CCTACGGCGC
    ATCGCCAACG TCACCTTGAT CGACCTTCGA GGATGCAAGC AGATCACTCG AAAAGCCTGC
    GAGCACTTCA TCTCAGACTT GTCCATCAAC AGTCTGTACT GCCTGTCTGA CGAGAAGCTG
    ATACAGAAGA TCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232230
    Clone ID Related Accession (Same CDS sequence) XM_024987455.1 , XM_024987456.1
    Accession Version XM_024987456.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTT
    CCTAATAAGT TCCGCTATCC ATTCTACTAC GAGATGTGCT GGTACGTGCT GGAGCGCTAT
    GTGTACTGCA TAACCAACCG CTCTCACCTA ACTAAGGAAT TTCAGAAAGA GTCTCTTAGC
    ATGGATTTGG AGTTAAATGG GTTGGAGTCT GGAAACGGGG ACGAGGAAGC AGTAGACCGA
    GAGCCCCGGC GCTTGAGCAG TAGACGTTCT GTCCTCACCA GCCCTGTGGC CAATGGGGTC
    AACCTGGATT ATGATGGACT GGGCAAAACC TGCAGAAGTC TTCCAAGTCT GAAGAAAACT
    TTGTCTGGGG ACTCATCCTC TGACTCTAGC CGGGGTTCCC AGAATGGCCA AGTGTGGGAT
    TCCCAGTGTA GCCCCCGAAA GGACCGACAG GTGCATCTGA CCCATTTTGA GCTTGAAGGC
    CTTCGTTGCC TTGTAGATAA ATTGGAATCT CTGCCACTGC ACAAGAAATG CGTCCCTACA
    GGGATAGAAG ACGAAGATGC TCTCATTGCT GATGTGAAGG TTTTGCTGGA GGAACTTGCC
    AACAGTGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC GAAAAGGGAT
    AAGCTTAAAT TCCCAACCCG GCCAAAGGTG CGGGTTCCTA CTATCCCCAT CACAAAGCCT
    CACACTATGA AACCAGCGCC GAGGTTAACA CCTGTGCGCC CAGCCGCTGC TTCTCCCATC
    GTGTCTGGAG CCAGACGGAG ACGAGTGCGA TGTCGAAAAT GCAAAGCCTG CGTGCAGGGA
    GAGTGTGGTG TTTGTCACTA CTGCAGGGAC ATGAAGAAGT TTGGGGGGCC TGGTCGCATG
    AAGCAGTCCT GTGTTCTCCG GCAGTGCTTG GCACCCAGAC TGCCTCACTC TGTCACATGT
    TCCCTCTGTG GAGAGGTGGA TCAAAATGAG GAGACACAGG ACTTTGAGAA GAAACTCATG
    GAATGCTGTA TCTGCAATGA GATTGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG
    TTGCTTAATG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGCTACCA AGAGGACAGC
    TCAGAAAAAG CCCAGTGTTC TTCTACACCC TGGGGCCCTT GGAAAAGAGG AGCCACAGCC
    AGGCTGTTTG AGGAGGGAAA TGAGAAGCGG AAAATGGAAG AGAGTGACGA AGAAGCCGTG
    CAAGCCAAAG TCCTCCGGCC CCTGCGGAGC TGCGACGAGC CCCTCACGCC CCCACCTCAC
    TCGCCCACCT CCATGCTGCA GCTCATCCAC GACCCGGCCT CCCCCCGGGG CGTGGTGACT
    CGGTCATCCC CTGGGGCCGG CCCCAGCGAC CACCACAGTG CCAGCCGCGA TGAGCGCTTC
    AAACGGCGGC AGTTGCTGCG GCTGCAGGCC ACAGAGCGCA CCATGGTACG GGAAAAGGAG
    AACAATCCCA GCGGCAAAAA GGAGCTGTCT GAAGTTGAGA AAGCCAAGAT CCGGGGATCG
    TACCTCACTG TCACGCTGCA GAGGCCCACC AAAGAGCTCC ACGGGACATC CATTGTGCCC
    AAGCTGCAGG CCATCACGGC CTCCTCTGCC AACCTTCGCC ATTCCCCCCG TGTGCTGGTC
    CAGCACTGCC CAGCCCGAAC CCCCCAGCGC GGGGATGAGG AGGGGCTGGG GGGAGAGGAG
    GAGGACGAGG AGGAGGAGGA GGAGGAAGAT GACAGTGCAG AGGAGGGGGG TGCGGCCAGG
    CTGAATGGCC GGGGCAGTTG GGCTCAGGAT GGAGACGAAA GCTGGATGCA GCGGGAGGTC
    TGGATGTCTG TCTTCCGCTA CCTCAGCCGC AGAGAACTTT GTGAATGTAT GCGAGTGTGC
    AAGACGTGGT ATAAATGGTG CTGCGACAAG AGACTTTGGA CAAAAATTGA CTTGAGTAGG
    TGTAAGGCTA TTGTACCCCA GGCTCTCAGT GGCATCATCA AGAGGCAGCC AGTTAGCCTT
    GACCTCAGTT GGACCAACAT CTCCAAAAAA CAGCTGACAT GGCTCGTCAA TAGGCTGCCA
    GGACTGAAAG ACCTCCTCCT AGCAGGCTGC TCCTGGTCTG CAGTCTCTGC CCTCAGCACC
    TCCAGCTGCC CCCTTCTCAG GACCCTTGAT CTTCGGTGGG CAGTAGGAAT CAAGGACCCT
    CAAATTCGGG ACTTGCTGAC TCCACCGGCG GATAAACCGG GCCAGGACAA TCGCAGCAAG
    CTCCGGAACA TGACCGACTT CCGGCTGGCA GGCCTTGACA TCACAGACGC CACGCTTCGT
    CTCATCATTC GCCACATGCC CCTCCTGTCT CGACTCGACC TCAGTCACTG CAGCCACCTG
    ACAGACCAGT CCTCCAATCT GCTCACTGCT GTTGGGTCTT CCACTCGTTA CTCCCTCACA
    GAGCTCAACA TGGCAGGTTG CAATAAACTG ACAGACCAGA CCCTGATCTA CCTACGGCGC
    ATCGCCAACG TCACCTTGAT CGACCTTCGA GGATGCAAGC AGATCACTCG AAAAGCCTGC
    GAGCACTTCA TCTCAGACTT GTCCATCAAC AGTCTGTACT GCCTGTCTGA CGAGAAGCTG
    ATACAGAAGA TCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843224.1
    CDS202..3759
    Translation

    Target ORF information:

    RefSeq Version XM_024987456.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2A (KDM2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTT
    CCTAATAAGT TCCGCTATCC ATTCTACTAC GAGATGTGCT GGTACGTGCT GGAGCGCTAT
    GTGTACTGCA TAACCAACCG CTCTCACCTA ACTAAGGAAT TTCAGAAAGA GTCTCTTAGC
    ATGGATTTGG AGTTAAATGG GTTGGAGTCT GGAAACGGGG ACGAGGAAGC AGTAGACCGA
    GAGCCCCGGC GCTTGAGCAG TAGACGTTCT GTCCTCACCA GCCCTGTGGC CAATGGGGTC
    AACCTGGATT ATGATGGACT GGGCAAAACC TGCAGAAGTC TTCCAAGTCT GAAGAAAACT
    TTGTCTGGGG ACTCATCCTC TGACTCTAGC CGGGGTTCCC AGAATGGCCA AGTGTGGGAT
    TCCCAGTGTA GCCCCCGAAA GGACCGACAG GTGCATCTGA CCCATTTTGA GCTTGAAGGC
    CTTCGTTGCC TTGTAGATAA ATTGGAATCT CTGCCACTGC ACAAGAAATG CGTCCCTACA
    GGGATAGAAG ACGAAGATGC TCTCATTGCT GATGTGAAGG TTTTGCTGGA GGAACTTGCC
    AACAGTGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC GAAAAGGGAT
    AAGCTTAAAT TCCCAACCCG GCCAAAGGTG CGGGTTCCTA CTATCCCCAT CACAAAGCCT
    CACACTATGA AACCAGCGCC GAGGTTAACA CCTGTGCGCC CAGCCGCTGC TTCTCCCATC
    GTGTCTGGAG CCAGACGGAG ACGAGTGCGA TGTCGAAAAT GCAAAGCCTG CGTGCAGGGA
    GAGTGTGGTG TTTGTCACTA CTGCAGGGAC ATGAAGAAGT TTGGGGGGCC TGGTCGCATG
    AAGCAGTCCT GTGTTCTCCG GCAGTGCTTG GCACCCAGAC TGCCTCACTC TGTCACATGT
    TCCCTCTGTG GAGAGGTGGA TCAAAATGAG GAGACACAGG ACTTTGAGAA GAAACTCATG
    GAATGCTGTA TCTGCAATGA GATTGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG
    TTGCTTAATG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGCTACCA AGAGGACAGC
    TCAGAAAAAG CCCAGTGTTC TTCTACACCC TGGGGCCCTT GGAAAAGAGG AGCCACAGCC
    AGGCTGTTTG AGGAGGGAAA TGAGAAGCGG AAAATGGAAG AGAGTGACGA AGAAGCCGTG
    CAAGCCAAAG TCCTCCGGCC CCTGCGGAGC TGCGACGAGC CCCTCACGCC CCCACCTCAC
    TCGCCCACCT CCATGCTGCA GCTCATCCAC GACCCGGCCT CCCCCCGGGG CGTGGTGACT
    CGGTCATCCC CTGGGGCCGG CCCCAGCGAC CACCACAGTG CCAGCCGCGA TGAGCGCTTC
    AAACGGCGGC AGTTGCTGCG GCTGCAGGCC ACAGAGCGCA CCATGGTACG GGAAAAGGAG
    AACAATCCCA GCGGCAAAAA GGAGCTGTCT GAAGTTGAGA AAGCCAAGAT CCGGGGATCG
    TACCTCACTG TCACGCTGCA GAGGCCCACC AAAGAGCTCC ACGGGACATC CATTGTGCCC
    AAGCTGCAGG CCATCACGGC CTCCTCTGCC AACCTTCGCC ATTCCCCCCG TGTGCTGGTC
    CAGCACTGCC CAGCCCGAAC CCCCCAGCGC GGGGATGAGG AGGGGCTGGG GGGAGAGGAG
    GAGGACGAGG AGGAGGAGGA GGAGGAAGAT GACAGTGCAG AGGAGGGGGG TGCGGCCAGG
    CTGAATGGCC GGGGCAGTTG GGCTCAGGAT GGAGACGAAA GCTGGATGCA GCGGGAGGTC
    TGGATGTCTG TCTTCCGCTA CCTCAGCCGC AGAGAACTTT GTGAATGTAT GCGAGTGTGC
    AAGACGTGGT ATAAATGGTG CTGCGACAAG AGACTTTGGA CAAAAATTGA CTTGAGTAGG
    TGTAAGGCTA TTGTACCCCA GGCTCTCAGT GGCATCATCA AGAGGCAGCC AGTTAGCCTT
    GACCTCAGTT GGACCAACAT CTCCAAAAAA CAGCTGACAT GGCTCGTCAA TAGGCTGCCA
    GGACTGAAAG ACCTCCTCCT AGCAGGCTGC TCCTGGTCTG CAGTCTCTGC CCTCAGCACC
    TCCAGCTGCC CCCTTCTCAG GACCCTTGAT CTTCGGTGGG CAGTAGGAAT CAAGGACCCT
    CAAATTCGGG ACTTGCTGAC TCCACCGGCG GATAAACCGG GCCAGGACAA TCGCAGCAAG
    CTCCGGAACA TGACCGACTT CCGGCTGGCA GGCCTTGACA TCACAGACGC CACGCTTCGT
    CTCATCATTC GCCACATGCC CCTCCTGTCT CGACTCGACC TCAGTCACTG CAGCCACCTG
    ACAGACCAGT CCTCCAATCT GCTCACTGCT GTTGGGTCTT CCACTCGTTA CTCCCTCACA
    GAGCTCAACA TGGCAGGTTG CAATAAACTG ACAGACCAGA CCCTGATCTA CCTACGGCGC
    ATCGCCAACG TCACCTTGAT CGACCTTCGA GGATGCAAGC AGATCACTCG AAAAGCCTGC
    GAGCACTTCA TCTCAGACTT GTCCATCAAC AGTCTGTACT GCCTGTCTGA CGAGAAGCTG
    ATACAGAAGA TCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232231
    Clone ID Related Accession (Same CDS sequence) XM_024987457.1
    Accession Version XM_024987457.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGACGAC GCTATGAAGA TGATGGCATT TCAGATGATG AAATTGAAGG AAAAAGAACT 
    TTTGACTTGG AAGAGAAGCT CCACACCAAC AAATATAATG CAAATTTTGT TACTTTTATG
    GAAGGAAAAG ATTTTAACGT AGAGTATATC CAGCGGGGTG GCTTAAGAGA TCCTCTGATT
    TTCAAGAATT CCGATGGACT TGGAATAAAG ATGCCAGATC CAGACTTCAC TGTGAATGAT
    GTCAAAATGT GTGTGGGGAG TCGTCGTATG GTGGATGTCA TGGATGTGAA CACACAGAAA
    GGCATTGAAA TGACCATGGC TCAGTGGACA CGCTACTATG AGACCCCAGA GGAGGAGCGA
    GAAAAACTCT ATAATGTCAT CAGCTTAGAG TTTAGCCACA CCAGGCTGGA GAACATGGTG
    CAGAGGCCCT CCACGGTGGA TTTCATTGAC TGGGTAGACA ATATGTGGCC AAGGCATTTG
    AAAGAAAGTC AGACTGAATC AACAAATGCC ATCTTGGAGA TGCAGTATCC TAAAGTGCAG
    AAGTACTGTC TGATGAGTGT TCGAGGCTGC TATACTGACT TCCATGTGGA CTTCGGTGGT
    ACCTCGGTTT GGTATCATAT CCATCAAGGG GGAAAGGTCT TCTGGCTCAT CCCCCCTACA
    GCCCACAACC TGGAGCTGTA CGAGAATTGG CTGCTGTCAG GGAAACAGGG AGACATCTTT
    CTGGGTGACC GGGTATCAGA TTGCCAACGC ATTGAGCTCA AGCAGGGCTA TACCTTCGTC
    ATTCCCTCAG GCTGGATTCA TGCTGTGTAT ACTCCTACAG ACACCTTGGT GTTTGGAGGC
    AATTTTTTGC ATAGCTTCAA TATCCCCATG CAGTTAAAGA TATACAACAT TGAAGATCGA
    ACACGGGTTC CTAATAAGTT CCGCTATCCA TTCTACTACG AGATGTGCTG GTACGTGCTG
    GAGCGCTATG TGTACTGCAT AACCAACCGC TCTCACCTAA CTAAGGAATT TCAGAAAGAG
    TCTCTTAGCA TGGATTTGGA GTTAAATGGG TTGGAGTCTG GAAACGGGGA CGAGGAAGCA
    GTAGACCGAG AGCCCCGGCG CTTGAGCAGT AGACGTTCTG TCCTCACCAG CCCTGTGGCC
    AATGGGGTCA ACCTGGATTA TGATGGACTG GGCAAAACCT GCAGAAGTCT TCCAAGTCTG
    AAGAAAACTT TGTCTGGGGA CTCATCCTCT GACTCTAGCC GGGGTTCCCA GAATGGCCAA
    GTGTGGGATT CCCAGTGTAG CCCCCGAAAG GACCGACAGG TGCATCTGAC CCATTTTGAG
    CTTGAAGGCC TTCGTTGCCT TGTAGATAAA TTGGAATCTC TGCCACTGCA CAAGAAATGC
    GTCCCTACAG GGATAGAAGA CGAAGATGCT CTCATTGCTG ATGTGAAGGT TTTGCTGGAG
    GAACTTGCCA ACAGTGATCC CAAGTTAGCC CTCACTGGAG TCCCTATAGT ACAGTGGCCG
    AAAAGGGATA AGCTTAAATT CCCAACCCGG CCAAAGGTGC GGGTTCCTAC TATCCCCATC
    ACAAAGCCTC ACACTATGAA ACCAGCGCCG AGGTTAACAC CTGTGCGCCC AGCCGCTGCT
    TCTCCCATCG TGTCTGGAGC CAGACGGAGA CGAGTGCGAT GTCGAAAATG CAAAGCCTGC
    GTGCAGGGAG AGTGTGGTGT TTGTCACTAC TGCAGGGACA TGAAGAAGTT TGGGGGGCCT
    GGTCGCATGA AGCAGTCCTG TGTTCTCCGG CAGTGCTTGG CACCCAGACT GCCTCACTCT
    GTCACATGTT CCCTCTGTGG AGAGGTGGAT CAAAATGAGG AGACACAGGA CTTTGAGAAG
    AAACTCATGG AATGCTGTAT CTGCAATGAG ATTGTTCATC CTGGCTGCCT CCAGATGGAT
    GGAGAGGGGT TGCTTAATGA GGAATTGCCA AATTGCTGGG AGTGTCCAAA GTGCTACCAA
    GAGGACAGCT CAGAAAAAGC CCAGTGTTCT TCTACACCCT GGGGCCCTTG GAAAAGAGGA
    GCCACAGCCA GGCTGTTTGA GGAGGGAAAT GAGAAGCGGA AAATGGAAGA GAGTGACGAA
    GAAGCCGTGC AAGCCAAAGT CCTCCGGCCC CTGCGGAGCT GCGACGAGCC CCTCACGCCC
    CCACCTCACT CGCCCACCTC CATGCTGCAG CTCATCCACG ACCCGGCCTC CCCCCGGGGC
    GTGGTGACTC GGTCATCCCC TGGGGCCGGC CCCAGCGACC ACCACAGTGC CAGCCGCGAT
    GAGCGCTTCA AACGGCGGCA GTTGCTGCGG CTGCAGGCCA CAGAGCGCAC CATGGTACGG
    GAAAAGGAGA ACAATCCCAG CGGCAAAAAG GAGCTGTCTG AAGTTGAGAA AGCCAAGATC
    CGGGGATCGT ACCTCACTGT CACGCTGCAG AGGCCCACCA AAGAGCTCCA CGGGACATCC
    ATTGTGCCCA AGCTGCAGGC CATCACGGCC TCCTCTGCCA ACCTTCGCCA TTCCCCCCGT
    GTGCTGGTCC AGCACTGCCC AGCCCGAACC CCCCAGCGCG GGGATGAGGA GGGGCTGGGG
    GGAGAGGAGG AGGACGAGGA GGAGGAGGAG GAGGAAGATG ACAGTGCAGA GGAGGGGGGT
    GCGGCCAGGC TGAATGGCCG GGGCAGTTGG GCTCAGGATG GAGACGAAAG CTGGATGCAG
    CGGGAGGTCT GGATGTCTGT CTTCCGCTAC CTCAGCCGCA GAGAACTTTG TGAATGTATG
    CGAGTGTGCA AGACGTGGTA TAAATGGTGC TGCGACAAGA GACTTTGGAC AAAAATTGAC
    TTGAGTAGGT GTAAGGCTAT TGTACCCCAG GCTCTCAGTG GCATCATCAA GAGGCAGCCA
    GTTAGCCTTG ACCTCAGTTG GACCAACATC TCCAAAAAAC AGCTGACATG GCTCGTCAAT
    AGGCTGCCAG GACTGAAAGA CCTCCTCCTA GCAGGCTGCT CCTGGTCTGC AGTCTCTGCC
    CTCAGCACCT CCAGCTGCCC CCTTCTCAGG ACCCTTGATC TTCGGTGGGC AGTAGGAATC
    AAGGACCCTC AAATTCGGGA CTTGCTGACT CCACCGGCGG ATAAACCGGG CCAGGACAAT
    CGCAGCAAGC TCCGGAACAT GACCGACTTC CGGCTGGCAG GCCTTGACAT CACAGACGCC
    ACGCTTCGTC TCATCATTCG CCACATGCCC CTCCTGTCTC GACTCGACCT CAGTCACTGC
    AGCCACCTGA CAGACCAGTC CTCCAATCTG CTCACTGCTG TTGGGTCTTC CACTCGTTAC
    TCCCTCACAG AGCTCAACAT GGCAGGTTGC AATAAACTGA CAGACCAGAC CCTGATCTAC
    CTACGGCGCA TCGCCAACGT CACCTTGATC GACCTTCGAG GATGCAAGCA GATCACTCGA
    AAAGCCTGCG AGCACTTCAT CTCAGACTTG TCCATCAACA GTCTGTACTG CCTGTCTGAC
    GAGAAGCTGA TACAGAAGAT CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843225.1
    CDS256..3762
    Translation

    Target ORF information:

    RefSeq Version XM_024987457.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2A (KDM2A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987457.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCGACGAC GCTATGAAGA TGATGGCATT TCAGATGATG AAATTGAAGG AAAAAGAACT 
    TTTGACTTGG AAGAGAAGCT CCACACCAAC AAATATAATG CAAATTTTGT TACTTTTATG
    GAAGGAAAAG ATTTTAACGT AGAGTATATC CAGCGGGGTG GCTTAAGAGA TCCTCTGATT
    TTCAAGAATT CCGATGGACT TGGAATAAAG ATGCCAGATC CAGACTTCAC TGTGAATGAT
    GTCAAAATGT GTGTGGGGAG TCGTCGTATG GTGGATGTCA TGGATGTGAA CACACAGAAA
    GGCATTGAAA TGACCATGGC TCAGTGGACA CGCTACTATG AGACCCCAGA GGAGGAGCGA
    GAAAAACTCT ATAATGTCAT CAGCTTAGAG TTTAGCCACA CCAGGCTGGA GAACATGGTG
    CAGAGGCCCT CCACGGTGGA TTTCATTGAC TGGGTAGACA ATATGTGGCC AAGGCATTTG
    AAAGAAAGTC AGACTGAATC AACAAATGCC ATCTTGGAGA TGCAGTATCC TAAAGTGCAG
    AAGTACTGTC TGATGAGTGT TCGAGGCTGC TATACTGACT TCCATGTGGA CTTCGGTGGT
    ACCTCGGTTT GGTATCATAT CCATCAAGGG GGAAAGGTCT TCTGGCTCAT CCCCCCTACA
    GCCCACAACC TGGAGCTGTA CGAGAATTGG CTGCTGTCAG GGAAACAGGG AGACATCTTT
    CTGGGTGACC GGGTATCAGA TTGCCAACGC ATTGAGCTCA AGCAGGGCTA TACCTTCGTC
    ATTCCCTCAG GCTGGATTCA TGCTGTGTAT ACTCCTACAG ACACCTTGGT GTTTGGAGGC
    AATTTTTTGC ATAGCTTCAA TATCCCCATG CAGTTAAAGA TATACAACAT TGAAGATCGA
    ACACGGGTTC CTAATAAGTT CCGCTATCCA TTCTACTACG AGATGTGCTG GTACGTGCTG
    GAGCGCTATG TGTACTGCAT AACCAACCGC TCTCACCTAA CTAAGGAATT TCAGAAAGAG
    TCTCTTAGCA TGGATTTGGA GTTAAATGGG TTGGAGTCTG GAAACGGGGA CGAGGAAGCA
    GTAGACCGAG AGCCCCGGCG CTTGAGCAGT AGACGTTCTG TCCTCACCAG CCCTGTGGCC
    AATGGGGTCA ACCTGGATTA TGATGGACTG GGCAAAACCT GCAGAAGTCT TCCAAGTCTG
    AAGAAAACTT TGTCTGGGGA CTCATCCTCT GACTCTAGCC GGGGTTCCCA GAATGGCCAA
    GTGTGGGATT CCCAGTGTAG CCCCCGAAAG GACCGACAGG TGCATCTGAC CCATTTTGAG
    CTTGAAGGCC TTCGTTGCCT TGTAGATAAA TTGGAATCTC TGCCACTGCA CAAGAAATGC
    GTCCCTACAG GGATAGAAGA CGAAGATGCT CTCATTGCTG ATGTGAAGGT TTTGCTGGAG
    GAACTTGCCA ACAGTGATCC CAAGTTAGCC CTCACTGGAG TCCCTATAGT ACAGTGGCCG
    AAAAGGGATA AGCTTAAATT CCCAACCCGG CCAAAGGTGC GGGTTCCTAC TATCCCCATC
    ACAAAGCCTC ACACTATGAA ACCAGCGCCG AGGTTAACAC CTGTGCGCCC AGCCGCTGCT
    TCTCCCATCG TGTCTGGAGC CAGACGGAGA CGAGTGCGAT GTCGAAAATG CAAAGCCTGC
    GTGCAGGGAG AGTGTGGTGT TTGTCACTAC TGCAGGGACA TGAAGAAGTT TGGGGGGCCT
    GGTCGCATGA AGCAGTCCTG TGTTCTCCGG CAGTGCTTGG CACCCAGACT GCCTCACTCT
    GTCACATGTT CCCTCTGTGG AGAGGTGGAT CAAAATGAGG AGACACAGGA CTTTGAGAAG
    AAACTCATGG AATGCTGTAT CTGCAATGAG ATTGTTCATC CTGGCTGCCT CCAGATGGAT
    GGAGAGGGGT TGCTTAATGA GGAATTGCCA AATTGCTGGG AGTGTCCAAA GTGCTACCAA
    GAGGACAGCT CAGAAAAAGC CCAGTGTTCT TCTACACCCT GGGGCCCTTG GAAAAGAGGA
    GCCACAGCCA GGCTGTTTGA GGAGGGAAAT GAGAAGCGGA AAATGGAAGA GAGTGACGAA
    GAAGCCGTGC AAGCCAAAGT CCTCCGGCCC CTGCGGAGCT GCGACGAGCC CCTCACGCCC
    CCACCTCACT CGCCCACCTC CATGCTGCAG CTCATCCACG ACCCGGCCTC CCCCCGGGGC
    GTGGTGACTC GGTCATCCCC TGGGGCCGGC CCCAGCGACC ACCACAGTGC CAGCCGCGAT
    GAGCGCTTCA AACGGCGGCA GTTGCTGCGG CTGCAGGCCA CAGAGCGCAC CATGGTACGG
    GAAAAGGAGA ACAATCCCAG CGGCAAAAAG GAGCTGTCTG AAGTTGAGAA AGCCAAGATC
    CGGGGATCGT ACCTCACTGT CACGCTGCAG AGGCCCACCA AAGAGCTCCA CGGGACATCC
    ATTGTGCCCA AGCTGCAGGC CATCACGGCC TCCTCTGCCA ACCTTCGCCA TTCCCCCCGT
    GTGCTGGTCC AGCACTGCCC AGCCCGAACC CCCCAGCGCG GGGATGAGGA GGGGCTGGGG
    GGAGAGGAGG AGGACGAGGA GGAGGAGGAG GAGGAAGATG ACAGTGCAGA GGAGGGGGGT
    GCGGCCAGGC TGAATGGCCG GGGCAGTTGG GCTCAGGATG GAGACGAAAG CTGGATGCAG
    CGGGAGGTCT GGATGTCTGT CTTCCGCTAC CTCAGCCGCA GAGAACTTTG TGAATGTATG
    CGAGTGTGCA AGACGTGGTA TAAATGGTGC TGCGACAAGA GACTTTGGAC AAAAATTGAC
    TTGAGTAGGT GTAAGGCTAT TGTACCCCAG GCTCTCAGTG GCATCATCAA GAGGCAGCCA
    GTTAGCCTTG ACCTCAGTTG GACCAACATC TCCAAAAAAC AGCTGACATG GCTCGTCAAT
    AGGCTGCCAG GACTGAAAGA CCTCCTCCTA GCAGGCTGCT CCTGGTCTGC AGTCTCTGCC
    CTCAGCACCT CCAGCTGCCC CCTTCTCAGG ACCCTTGATC TTCGGTGGGC AGTAGGAATC
    AAGGACCCTC AAATTCGGGA CTTGCTGACT CCACCGGCGG ATAAACCGGG CCAGGACAAT
    CGCAGCAAGC TCCGGAACAT GACCGACTTC CGGCTGGCAG GCCTTGACAT CACAGACGCC
    ACGCTTCGTC TCATCATTCG CCACATGCCC CTCCTGTCTC GACTCGACCT CAGTCACTGC
    AGCCACCTGA CAGACCAGTC CTCCAATCTG CTCACTGCTG TTGGGTCTTC CACTCGTTAC
    TCCCTCACAG AGCTCAACAT GGCAGGTTGC AATAAACTGA CAGACCAGAC CCTGATCTAC
    CTACGGCGCA TCGCCAACGT CACCTTGATC GACCTTCGAG GATGCAAGCA GATCACTCGA
    AAAGCCTGCG AGCACTTCAT CTCAGACTTG TCCATCAACA GTCTGTACTG CCTGTCTGAC
    GAGAAGCTGA TACAGAAGAT CAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232232
    Clone ID Related Accession (Same CDS sequence) XM_024987458.1
    Accession Version XM_024987458.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTT
    CCTAATAAGT TCCGCTATCC ATTCTACTAC GAGATGTGCT GGTACGTGCT GGAGCGCTAT
    GTGTACTGCA TAACCAACCG CTCTCACCTA ACTAAGGAAT TTCAGAAAGA GTCTCTTAGC
    ATGGATTTGG AGTTAAATGG GTTGGAGTCT GGAAACGGGG ACGAGGAAGC AGTAGACCGA
    GAGCCCCGGC GCTTGAGCAG TAGACGTTCT GTCCTCACCA GCCCTGTGGC CAATGGGGTC
    AACCTGGATT ATGATGGACT GGGCAAAACC TGCAGAAGTC TTCCAAGTCT GAAGAAAACT
    TTGTCTGGGG ACTCATCCTC TGACTCTAGC CGGGGTTCCC AGAATGGCCA AGTGTGGGAT
    TCCCAGTGTA GCCCCCGAAA GGACCGACAG GTGCATCTGA CCCATTTTGA GCTTGAAGGC
    CTTCGTTGCC TTGTAGATAA ATTGGAATCT CTGCCACTGC ACAAGAAATG CGTCCCTACA
    GGGATAGAAG ACGAAGATGC TCTCATTGCT GATGTGAAGG TTTTGCTGGA GGAACTTGCC
    AACAGTGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC GAAAAGGGAT
    AAGCTTAAAT TCCCAACCCG GCCAAAGGTG CGGGTTCCTA CTATCCCCAT CACAAAGCCT
    CACACTATGA AACCAGCGCC GAGGTTAACA CCTGTGCGCC CAGCCGCTGC TTCTCCCATC
    GTGTCTGGAG CCAGACGGAG ACGAGTGCGA TGTCGAAAAT GCAAAGCCTG CGTGCAGGGA
    GAGTGTGGTG TTTGTCACTA CTGCAGGGAC ATGAAGAAGT TTGGGGGGCC TGGTCGCATG
    AAGCAGTCCT GTGTTCTCCG GCAGTGCTTG GCACCCAGAC TGCCTCACTC TGTCACATGT
    TCCCTCTGTG GAGAGGTGGA TCAAAATGAG GAGACACAGG ACTTTGAGAA GAAACTCATG
    GAATGCTGTA TCTGCAATGA GATTGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG
    TTGCTTAATG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGCTACCA AGAGGACAGC
    TCAGAAAAAG CCCAGAAGCG GAAAATGGAA GAGAGTGACG AAGAAGCCGT GCAAGCCAAA
    GTCCTCCGGC CCCTGCGGAG CTGCGACGAG CCCCTCACGC CCCCACCTCA CTCGCCCACC
    TCCATGCTGC AGCTCATCCA CGACCCGGCC TCCCCCCGGG GCGTGGTGAC TCGGTCATCC
    CCTGGGGCCG GCCCCAGCGA CCACCACAGT GCCAGCCGCG ATGAGCGCTT CAAACGGCGG
    CAGTTGCTGC GGCTGCAGGC CACAGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC
    AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT
    GTCACGCTGC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG
    GCCATCACGG CCTCCTCTGC CAACCTTCGC CATTCCCCCC GTGTGCTGGT CCAGCACTGC
    CCAGCCCGAA CCCCCCAGCG CGGGGATGAG GAGGGGCTGG GGGGAGAGGA GGAGGACGAG
    GAGGAGGAGG AGGAGGAAGA TGACAGTGCA GAGGAGGGGG GTGCGGCCAG GCTGAATGGC
    CGGGGCAGTT GGGCTCAGGA TGGAGACGAA AGCTGGATGC AGCGGGAGGT CTGGATGTCT
    GTCTTCCGCT ACCTCAGCCG CAGAGAACTT TGTGAATGTA TGCGAGTGTG CAAGACGTGG
    TATAAATGGT GCTGCGACAA GAGACTTTGG ACAAAAATTG ACTTGAGTAG GTGTAAGGCT
    ATTGTACCCC AGGCTCTCAG TGGCATCATC AAGAGGCAGC CAGTTAGCCT TGACCTCAGT
    TGGACCAACA TCTCCAAAAA ACAGCTGACA TGGCTCGTCA ATAGGCTGCC AGGACTGAAA
    GACCTCCTCC TAGCAGGCTG CTCCTGGTCT GCAGTCTCTG CCCTCAGCAC CTCCAGCTGC
    CCCCTTCTCA GGACCCTTGA TCTTCGGTGG GCAGTAGGAA TCAAGGACCC TCAAATTCGG
    GACTTGCTGA CTCCACCGGC GGATAAACCG GGCCAGGACA ATCGCAGCAA GCTCCGGAAC
    ATGACCGACT TCCGGCTGGC AGGCCTTGAC ATCACAGACG CCACGCTTCG TCTCATCATT
    CGCCACATGC CCCTCCTGTC TCGACTCGAC CTCAGTCACT GCAGCCACCT GACAGACCAG
    TCCTCCAATC TGCTCACTGC TGTTGGGTCT TCCACTCGTT ACTCCCTCAC AGAGCTCAAC
    ATGGCAGGTT GCAATAAACT GACAGACCAG ACCCTGATCT ACCTACGGCG CATCGCCAAC
    GTCACCTTGA TCGACCTTCG AGGATGCAAG CAGATCACTC GAAAAGCCTG CGAGCACTTC
    ATCTCAGACT TGTCCATCAA CAGTCTGTAC TGCCTGTCTG ACGAGAAGCT GATACAGAAG
    ATCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024843226.1
    CDS496..3984
    Translation

    Target ORF information:

    RefSeq Version XM_024987458.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine demethylase 2A (KDM2A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987458.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTT
    CCTAATAAGT TCCGCTATCC ATTCTACTAC GAGATGTGCT GGTACGTGCT GGAGCGCTAT
    GTGTACTGCA TAACCAACCG CTCTCACCTA ACTAAGGAAT TTCAGAAAGA GTCTCTTAGC
    ATGGATTTGG AGTTAAATGG GTTGGAGTCT GGAAACGGGG ACGAGGAAGC AGTAGACCGA
    GAGCCCCGGC GCTTGAGCAG TAGACGTTCT GTCCTCACCA GCCCTGTGGC CAATGGGGTC
    AACCTGGATT ATGATGGACT GGGCAAAACC TGCAGAAGTC TTCCAAGTCT GAAGAAAACT
    TTGTCTGGGG ACTCATCCTC TGACTCTAGC CGGGGTTCCC AGAATGGCCA AGTGTGGGAT
    TCCCAGTGTA GCCCCCGAAA GGACCGACAG GTGCATCTGA CCCATTTTGA GCTTGAAGGC
    CTTCGTTGCC TTGTAGATAA ATTGGAATCT CTGCCACTGC ACAAGAAATG CGTCCCTACA
    GGGATAGAAG ACGAAGATGC TCTCATTGCT GATGTGAAGG TTTTGCTGGA GGAACTTGCC
    AACAGTGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC GAAAAGGGAT
    AAGCTTAAAT TCCCAACCCG GCCAAAGGTG CGGGTTCCTA CTATCCCCAT CACAAAGCCT
    CACACTATGA AACCAGCGCC GAGGTTAACA CCTGTGCGCC CAGCCGCTGC TTCTCCCATC
    GTGTCTGGAG CCAGACGGAG ACGAGTGCGA TGTCGAAAAT GCAAAGCCTG CGTGCAGGGA
    GAGTGTGGTG TTTGTCACTA CTGCAGGGAC ATGAAGAAGT TTGGGGGGCC TGGTCGCATG
    AAGCAGTCCT GTGTTCTCCG GCAGTGCTTG GCACCCAGAC TGCCTCACTC TGTCACATGT
    TCCCTCTGTG GAGAGGTGGA TCAAAATGAG GAGACACAGG ACTTTGAGAA GAAACTCATG
    GAATGCTGTA TCTGCAATGA GATTGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG
    TTGCTTAATG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGCTACCA AGAGGACAGC
    TCAGAAAAAG CCCAGAAGCG GAAAATGGAA GAGAGTGACG AAGAAGCCGT GCAAGCCAAA
    GTCCTCCGGC CCCTGCGGAG CTGCGACGAG CCCCTCACGC CCCCACCTCA CTCGCCCACC
    TCCATGCTGC AGCTCATCCA CGACCCGGCC TCCCCCCGGG GCGTGGTGAC TCGGTCATCC
    CCTGGGGCCG GCCCCAGCGA CCACCACAGT GCCAGCCGCG ATGAGCGCTT CAAACGGCGG
    CAGTTGCTGC GGCTGCAGGC CACAGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC
    AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT
    GTCACGCTGC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG
    GCCATCACGG CCTCCTCTGC CAACCTTCGC CATTCCCCCC GTGTGCTGGT CCAGCACTGC
    CCAGCCCGAA CCCCCCAGCG CGGGGATGAG GAGGGGCTGG GGGGAGAGGA GGAGGACGAG
    GAGGAGGAGG AGGAGGAAGA TGACAGTGCA GAGGAGGGGG GTGCGGCCAG GCTGAATGGC
    CGGGGCAGTT GGGCTCAGGA TGGAGACGAA AGCTGGATGC AGCGGGAGGT CTGGATGTCT
    GTCTTCCGCT ACCTCAGCCG CAGAGAACTT TGTGAATGTA TGCGAGTGTG CAAGACGTGG
    TATAAATGGT GCTGCGACAA GAGACTTTGG ACAAAAATTG ACTTGAGTAG GTGTAAGGCT
    ATTGTACCCC AGGCTCTCAG TGGCATCATC AAGAGGCAGC CAGTTAGCCT TGACCTCAGT
    TGGACCAACA TCTCCAAAAA ACAGCTGACA TGGCTCGTCA ATAGGCTGCC AGGACTGAAA
    GACCTCCTCC TAGCAGGCTG CTCCTGGTCT GCAGTCTCTG CCCTCAGCAC CTCCAGCTGC
    CCCCTTCTCA GGACCCTTGA TCTTCGGTGG GCAGTAGGAA TCAAGGACCC TCAAATTCGG
    GACTTGCTGA CTCCACCGGC GGATAAACCG GGCCAGGACA ATCGCAGCAA GCTCCGGAAC
    ATGACCGACT TCCGGCTGGC AGGCCTTGAC ATCACAGACG CCACGCTTCG TCTCATCATT
    CGCCACATGC CCCTCCTGTC TCGACTCGAC CTCAGTCACT GCAGCCACCT GACAGACCAG
    TCCTCCAATC TGCTCACTGC TGTTGGGTCT TCCACTCGTT ACTCCCTCAC AGAGCTCAAC
    ATGGCAGGTT GCAATAAACT GACAGACCAG ACCCTGATCT ACCTACGGCG CATCGCCAAC
    GTCACCTTGA TCGACCTTCG AGGATGCAAG CAGATCACTC GAAAAGCCTG CGAGCACTTC
    ATCTCAGACT TGTCCATCAA CAGTCTGTAC TGCCTGTCTG ACGAGAAGCT GATACAGAAG
    ATCAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89123
    Clone ID Related Accession (Same CDS sequence) XM_015461297.1
    Accession Version XM_015461297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3486bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104647.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTA
    AGTAACTGTG TAAGAATTAA ATGTGAAGAT GTTTCATATA TCTATTTTTA CTCTGCTTAC
    TGTGTAACAC TCAAACTATT ATACGGAGCC GCCCGCAGCG GGGCGGCTCT CACGGTGTGG
    GCTGGGGCAG AGGCCGCCGC AGTTGTGGAA GTAGCTGAGA TGAACAGACG GATGCCTAGG
    GGAGGGGCAG CGGGGATAGG TGAGGCTGGC CGGCGGTGGG GAGAGAACGC AACTCCAGAG
    GCGCTCGTCT CCGGGCCGGG CTCTGCTGCA GAGGCTGAAC TTCAGGCGAC TCGCTGTCTG
    CCCGGGCACG GGGAGAGCCC TGTGCGCAGC CCGGGGGAGC GCGGCCGTGA CAGGGAGGTG
    ACCCTGAGGG CCGGGGTGTG GACCGCGACT GGGATTTTTA CTAGAGGCAG AACACCTAAC
    TCCTTCAGCA CAACTTCCAA TACAGAAAGC AAAACAAAGA CGCTGGGGAA ACTTCATCCA
    AGAGCCTCGG CAGCTCCAGA GGACGGGAAA CACAAGGTTT TGCTGGAGGA ACTTGCCAAC
    AGTGATCCCA AGTTAGCCCT CACTGGAGTC CCTATAGTAC AGTGGCCGAA AAGGGATAAG
    CTTAAATTCC CAACCCGGCC AAAGGTGCGG GTTCCTACTA TCCCCATCAC AAAGCCTCAC
    ACTATGAAAC CAGCGCCGAG GTTAACACCT GTGCGCCCAG CCGCTGCTTC TCCCATCGTG
    TCTGGAGCCA GACGGAGACG AGTGCGATGT CGAAAATGCA AAGCCTGCGT GCAGGGAGAG
    TGTGGTGTTT GTCACTACTG CAGGGACATG AAGAAGTTTG GGGGGCCTGG TCGCATGAAG
    CAGTCCTGTG TTCTCCGGCA GTGCTTGGCA CCCAGACTGC CTCACTCTGT CACATGTTCC
    CTCTGTGGAG AGGTGGATCA AAATGAGGAG ACACAGGACT TTGAGAAGAA ACTCATGGAA
    TGCTGTATCT GCAATGAGAT TGTTCATCCT GGCTGCCTCC AGATGGATGG AGAGGGGTTG
    CTTAATGAGG AATTGCCAAA TTGCTGGGAG TGTCCAAAGT GCTACCAAGA GGACAGCTCA
    GAAAAAGCCC AGAAGCGGAA AATGGAAGAG AGTGACGAAG AAGCCGTGCA AGCCAAAGTC
    CTCCGGCCCC TGCGGAGCTG CGACGAGCCC CTCACGCCCC CACCTCACTC GCCCACCTCC
    ATGCTGCAGC TCATCCACGA CCCGGCCTCC CCCCGGGGCG TGGTGACTCG GTCATCCCCT
    GGGGCCGGCC CCAGCGACCA CCACAGTGCC AGCCGCGATG AGCGCTTCAA ACGGCGGCAG
    TTGCTGCGGC TGCAGGCCAC AGAGCGCACC ATGGTACGGG AAAAGGAGAA CAATCCCAGC
    GGCAAAAAGG AGCTGTCTGA AGTTGAGAAA GCCAAGATCC GGGGATCGTA CCTCACTGTC
    ACGCTGCAGA GGCCCACCAA AGAGCTCCAC GGGACATCCA TTGTGCCCAA GCTGCAGGCC
    ATCACGGCCT CCTCTGCCAA CCTTCGCCAT TCCCCCCGTG TGCTGGTCCA GCACTGCCCA
    GCCCGAACCC CCCAGCGCGG GGATGAGGAG GGGCTGGGGG GAGAGGAGGA GGACGAGGAG
    GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CGGCCAGGCT GAATGGCCGG
    GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC
    TTCCGCTACC TCAGCCGCAG AGAACTTTGT GAATGTATGC GAGTGTGCAA GACGTGGTAT
    AAATGGTGCT GCGACAAGAG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCTATT
    GTACCCCAGG CTCTCAGTGG CATCATCAAG AGGCAGCCAG TTAGCCTTGA CCTCAGTTGG
    ACCAACATCT CCAAAAAACA GCTGACATGG CTCGTCAATA GGCTGCCAGG ACTGAAAGAC
    CTCCTCCTAG CAGGCTGCTC CTGGTCTGCA GTCTCTGCCC TCAGCACCTC CAGCTGCCCC
    CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATCA AGGACCCTCA AATTCGGGAC
    TTGCTGACTC CACCGGCGGA TAAACCGGGC CAGGACAATC GCAGCAAGCT CCGGAACATG
    ACCGACTTCC GGCTGGCAGG CCTTGACATC ACAGACGCCA CGCTTCGTCT CATCATTCGC
    CACATGCCCC TCCTGTCTCG ACTCGACCTC AGTCACTGCA GCCACCTGAC AGACCAGTCC
    TCCAATCTGC TCACTGCTGT TGGGTCTTCC ACTCGTTACT CCCTCACAGA GCTCAACATG
    GCAGGTTGCA ATAAACTGAC AGACCAGACC CTGATCTACC TACGGCGCAT CGCCAACGTC
    ACCTTGATCG ACCTTCGAGG ATGCAAGCAG ATCACTCGAA AAGCCTGCGA GCACTTCATC
    TCAGACTTGT CCATCAACAG TCTGTACTGC CTGTCTGACG AGAAGCTGAT ACAGAAGATC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316783.1
    CDS306..3791
    Translation

    Target ORF information:

    RefSeq Version XM_015461297.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2A (KDM2A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTA
    AGTAACTGTG TAAGAATTAA ATGTGAAGAT GTTTCATATA TCTATTTTTA CTCTGCTTAC
    TGTGTAACAC TCAAACTATT ATACGGAGCC GCCCGCAGCG GGGCGGCTCT CACGGTGTGG
    GCTGGGGCAG AGGCCGCCGC AGTTGTGGAA GTAGCTGAGA TGAACAGACG GATGCCTAGG
    GGAGGGGCAG CGGGGATAGG TGAGGCTGGC CGGCGGTGGG GAGAGAACGC AACTCCAGAG
    GCGCTCGTCT CCGGGCCGGG CTCTGCTGCA GAGGCTGAAC TTCAGGCGAC TCGCTGTCTG
    CCCGGGCACG GGGAGAGCCC TGTGCGCAGC CCGGGGGAGC GCGGCCGTGA CAGGGAGGTG
    ACCCTGAGGG CCGGGGTGTG GACCGCGACT GGGATTTTTA CTAGAGGCAG AACACCTAAC
    TCCTTCAGCA CAACTTCCAA TACAGAAAGC AAAACAAAGA CGCTGGGGAA ACTTCATCCA
    AGAGCCTCGG CAGCTCCAGA GGACGGGAAA CACAAGGTTT TGCTGGAGGA ACTTGCCAAC
    AGTGATCCCA AGTTAGCCCT CACTGGAGTC CCTATAGTAC AGTGGCCGAA AAGGGATAAG
    CTTAAATTCC CAACCCGGCC AAAGGTGCGG GTTCCTACTA TCCCCATCAC AAAGCCTCAC
    ACTATGAAAC CAGCGCCGAG GTTAACACCT GTGCGCCCAG CCGCTGCTTC TCCCATCGTG
    TCTGGAGCCA GACGGAGACG AGTGCGATGT CGAAAATGCA AAGCCTGCGT GCAGGGAGAG
    TGTGGTGTTT GTCACTACTG CAGGGACATG AAGAAGTTTG GGGGGCCTGG TCGCATGAAG
    CAGTCCTGTG TTCTCCGGCA GTGCTTGGCA CCCAGACTGC CTCACTCTGT CACATGTTCC
    CTCTGTGGAG AGGTGGATCA AAATGAGGAG ACACAGGACT TTGAGAAGAA ACTCATGGAA
    TGCTGTATCT GCAATGAGAT TGTTCATCCT GGCTGCCTCC AGATGGATGG AGAGGGGTTG
    CTTAATGAGG AATTGCCAAA TTGCTGGGAG TGTCCAAAGT GCTACCAAGA GGACAGCTCA
    GAAAAAGCCC AGAAGCGGAA AATGGAAGAG AGTGACGAAG AAGCCGTGCA AGCCAAAGTC
    CTCCGGCCCC TGCGGAGCTG CGACGAGCCC CTCACGCCCC CACCTCACTC GCCCACCTCC
    ATGCTGCAGC TCATCCACGA CCCGGCCTCC CCCCGGGGCG TGGTGACTCG GTCATCCCCT
    GGGGCCGGCC CCAGCGACCA CCACAGTGCC AGCCGCGATG AGCGCTTCAA ACGGCGGCAG
    TTGCTGCGGC TGCAGGCCAC AGAGCGCACC ATGGTACGGG AAAAGGAGAA CAATCCCAGC
    GGCAAAAAGG AGCTGTCTGA AGTTGAGAAA GCCAAGATCC GGGGATCGTA CCTCACTGTC
    ACGCTGCAGA GGCCCACCAA AGAGCTCCAC GGGACATCCA TTGTGCCCAA GCTGCAGGCC
    ATCACGGCCT CCTCTGCCAA CCTTCGCCAT TCCCCCCGTG TGCTGGTCCA GCACTGCCCA
    GCCCGAACCC CCCAGCGCGG GGATGAGGAG GGGCTGGGGG GAGAGGAGGA GGACGAGGAG
    GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CGGCCAGGCT GAATGGCCGG
    GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC
    TTCCGCTACC TCAGCCGCAG AGAACTTTGT GAATGTATGC GAGTGTGCAA GACGTGGTAT
    AAATGGTGCT GCGACAAGAG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCTATT
    GTACCCCAGG CTCTCAGTGG CATCATCAAG AGGCAGCCAG TTAGCCTTGA CCTCAGTTGG
    ACCAACATCT CCAAAAAACA GCTGACATGG CTCGTCAATA GGCTGCCAGG ACTGAAAGAC
    CTCCTCCTAG CAGGCTGCTC CTGGTCTGCA GTCTCTGCCC TCAGCACCTC CAGCTGCCCC
    CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATCA AGGACCCTCA AATTCGGGAC
    TTGCTGACTC CACCGGCGGA TAAACCGGGC CAGGACAATC GCAGCAAGCT CCGGAACATG
    ACCGACTTCC GGCTGGCAGG CCTTGACATC ACAGACGCCA CGCTTCGTCT CATCATTCGC
    CACATGCCCC TCCTGTCTCG ACTCGACCTC AGTCACTGCA GCCACCTGAC AGACCAGTCC
    TCCAATCTGC TCACTGCTGT TGGGTCTTCC ACTCGTTACT CCCTCACAGA GCTCAACATG
    GCAGGTTGCA ATAAACTGAC AGACCAGACC CTGATCTACC TACGGCGCAT CGCCAACGTC
    ACCTTGATCG ACCTTCGAGG ATGCAAGCAG ATCACTCGAA AAGCCTGCGA GCACTTCATC
    TCAGACTTGT CCATCAACAG TCTGTACTGC CTGTCTGACG AGAAGCTGAT ACAGAAGATC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa95290
    Clone ID Related Accession (Same CDS sequence) XM_015470056.1
    Accession Version XM_015470056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product lysine-specific demethylase 2A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646807.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 19% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTA
    AGTAACTGTG TAAGAATTAA ATGTGAAGAT GTTTCATATA TCTATTTTTA CTCTGCTTAC
    TGTGTAACAC TCAAACTATT ATACTTTAAC ATATGGCTGG TGAGGACAGA ACGTCTACTG
    CTCAAGCGCC GGGGCTCTCG GTCTACTGCT TCCTCGTCCC CGTTTCCAGA CTCCAACCCA
    TTTAACTCCA AATCTGCCAG TATGAAGAGA AAGTGTGGTG TGAATGTGCC ACTCGGCCAT
    GTGATCAACA AGAGAAATAG CCCACAACCT GAGCTGTACG AGAATTGGCT GCTGTCAGGG
    AAACAGGGAG ACATCTTTCT GGGTGACCGG GTATCAGATT GCCAACGCAT TGAGCTCAAG
    CAGGCTATAC CTTCGTTGTC ACCCTCAGGC TGGATTCATG CTGTGTATAC TCCTACAGAC
    ACCTTGGTGT TTGGAGGCAA TTTTTTGCAT AGCTTCAATA TCCCCATGCA GTTAAAGATA
    TACAACATTG AAGATCGAAC ACGGGGAGCC GCCCGCAGCG GGGCGGCTCT CACGGTGTGG
    GCTGGGGCAG AGGCCGCCGC AGTTGTGGAA GTAGCTGAGA TGAACAGACG GATGCCTAGG
    GGAGGGGCAG CGGGGATAGG TGAGGCTGGC CGGCGGTGGG GAGAGAACGC AACTCCAGAG
    GCGCTCGTCT CCGGGCCGGG CTCTGCTGCA GAGGCTGAAC TTCAGGCGAC TCGCTGTCTG
    CCCGGGCACG GGGAGAGCCC TGTGCGCAGC CCGGGGGAGC GCGGCCGTGA CAGGGAGGTG
    ACCCTGAGGG CCGGGGTGTG GACCGCGACT GGGATTTTTA CTAGAGGCAG AACACCTAAC
    TCCTTCAGCA CAACTTCCAA TACAGAAAGC AAAACAAAGA CGCTGGGGAA ACTTCATCCA
    AGAGCCTCGG CAGCTCCAGA GGACGGGAAA CACAAGGTTT TGCTGGAGGA ACTTGCCAAC
    AGTGATCCCA AGTTAGCCCT CACTGGAGTC CCTATAGTAC AGTGGCCGAA AAGGGATAAG
    CTTAAATTCC CAACCCGGCC AAAGGTGCGG GTTCCTACTA TCCCCATCAC AAAGCCTCAC
    ACTATGAAAC CAGCGCCGAG GTTAACACCT GTGCGCCCAG CCGCTGCTTC TCCCATCGTG
    TCTGGAGCCA GACGGAGACG AGTGCGATGT CGAAAATGCA AAGCCTGCGT GCAGGGAGAG
    TGTGGTGTTT GTCACTACTG CAGGGACATG AAGAAGTTTG GGGGGCCTGG TCGCATGAAG
    CAGTCCTGTG TTCTCCGGCA GTGCTTGGCA CCCAGACTGC CTCACTCTGT CACATGTTCC
    CTCTGTGGAG AGGTGGATCA AAATGAGGAG ACACAGGACT TTGAGAAGAA ACTCATGGAA
    TGCTGTATCT GCAATGAGAT TGTTCATCCT GGCTGCCTCC AGATGGATGG AGAGGGGTTG
    CTTAATGAGG AATTGCCAAA TTGCTGGGAG TGTCCAAAGT GCTACCAAGA GGACAGCTCA
    GAAAAAGCCC AGAAGCGGAA AATGGAAGAG AGTGACGAAG AAGCCGTGCA AGCCAAAGTC
    CTCCGGCCCC TGCGGAGCTG CGACGAGCCC CTCACGCCCC CACCTCACTC GCCCACCTCC
    ATGCTGCAGC TCATCCACGA CCCGGCCTCC CCCCGGGGCG TGGTGACTCG GTCATCCCCT
    GGGGCCGGCC CCAGCGACCA CCACAGTGCC AGCCGCGATG AGCGCTTCAA ACGGCGGCAG
    TTGCTGCGGC TGCAGGCCAC AGAGCGCACC ATGGTACGGG AAAAGGAGAA CAATCCCAGC
    GGCAAAAAGG AGCTGTCTGA AGTTGAGAAA GCCAAGATCC GGGGATCGTA CCTCACTGTC
    ACGCTGCAGA GGCCCACCAA AGAGCTCCAC GGGACATCCA TTGTGCCCAA GCTGCAGGCC
    ATCACGGCCT CCTCTGCCAA CCTTCGCCAT TCCCCCCGTG TGCTGGTCCA GCACTGCCCA
    GCCCGAACCC CCCAGCGCGG GGATGAGGAG GGGCTGGGGG GAGAGGAGGA GGACGAGGAG
    GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CGGCCAGGCT GAATGGCCGG
    GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC
    TTCCGCTACC TCAGCCGCAG AGAACTTTGT GAATGTATGC GAGTGTGCAA GACGTGGTAT
    AAATGGTGCT GCGACAAGAG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCTATT
    GTACCCCAGG CTCTCAGTGG CATCATCAAG AGGCAGCCAG TTAGCCTTGA CCTCAGTTGG
    ACCAACATCT CCAAAAAACA GCTGACATGG CTCGTCAATA GGCTGCCAGG ACTGAAAGAC
    CTCCTCCTAG CAGGCTGCTC CTGGTCTGCA GTCTCTGCCC TCAGCACCTC CAGCTGCCCC
    CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATCA AGGACCCTCA AATTCGGGAC
    TTGCTGACTC CACCGGCGGA TAAACCGGGC CAGGACAATC GCAGCAAGCT CCGGAACATG
    ACCGACTTCC GGCTGGCAGG CCTTGACATC ACAGACGCCA CGCTTCGTCT CATCATTCGC
    CACATGCCCC TCCTGTCTCG ACTCGACCTC AGTCACTGCA GCCACCTGAC AGACCAGTCC
    TCCAATCTGC TCACTGCTGT TGGGTCTTCC ACTCGTTACT CCCTCACAGA GCTCAACATG
    GCAGGTTGCA ATAAACTGAC AGACCAGACC CTGATCTACC TACGGCGCAT CGCCAACGTC
    ACCTTGATCG ACCTTCGAGG ATGCAAGCAG ATCACTCGAA AAGCCTGCGA GCACTTCATC
    TCAGACTTGT CCATCAACAG TCTGTACTGC CTGTCTGACG AGAAGCTGAT ACAGAAGATC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325542.1
    CDS306..4211
    Translation

    Target ORF information:

    RefSeq Version XM_015470056.1
    Organism Bos taurus(cattle)
    Definition Bos taurus lysine (K)-specific demethylase 2A (KDM2A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGAACCCG AAGAAGAAAG GATTCGTTAC AGCCAGAGAT TGCGTGGTAC CATGCGACGA 
    CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GAAAAAGAAC TTTTGACTTG
    GAAGAGAAGC TCCACACCAA CAAATATAAT GCAAATTTTG TTACTTTTAT GGAAGGAAAA
    GATTTTAACG TAGAGTATAT CCAGCGGGGT GGCTTAAGAG ATCCTCTGAT TTTCAAGAAT
    TCCGATGGAC TTGGAATAAA GATGCCAGAT CCAGACTTCA CTGTGAATGA TGTCAAAATG
    TGTGTGGGGA GTCGTCGTAT GGTGGATGTC ATGGATGTGA ACACACAGAA AGGCATTGAA
    ATGACCATGG CTCAGTGGAC ACGCTACTAT GAGACCCCAG AGGAGGAGCG AGAAAAACTC
    TATAATGTCA TCAGCTTAGA GTTTAGCCAC ACCAGGCTGG AGAACATGGT GCAGAGGCCC
    TCCACGGTGG ATTTCATTGA CTGGGTAGAC AATATGTGGC CAAGGCATTT GAAAGAAAGT
    CAGACTGAAT CAACAAATGC CATCTTGGAG ATGCAGTATC CTAAAGTGCA GAAGTACTGT
    CTGATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ACTTCGGTGG TACCTCGGTT
    TGGTATCATA TCCATCAAGG GGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC
    CTGGAGCTGT ACGAGAATTG GCTGCTGTCA GGGAAACAGG GAGACATCTT TCTGGGTGAC
    CGGGTATCAG ATTGCCAACG CATTGAGCTC AAGCAGGGCT ATACCTTCGT CATTCCCTCA
    GGCTGGATTC ATGCTGTGTA TACTCCTACA GACACCTTGG TGTTTGGAGG CAATTTTTTG
    CATAGCTTCA ATATCCCCAT GCAGTTAAAG ATATACAACA TTGAAGATCG AACACGGGTA
    AGTAACTGTG TAAGAATTAA ATGTGAAGAT GTTTCATATA TCTATTTTTA CTCTGCTTAC
    TGTGTAACAC TCAAACTATT ATACTTTAAC ATATGGCTGG TGAGGACAGA ACGTCTACTG
    CTCAAGCGCC GGGGCTCTCG GTCTACTGCT TCCTCGTCCC CGTTTCCAGA CTCCAACCCA
    TTTAACTCCA AATCTGCCAG TATGAAGAGA AAGTGTGGTG TGAATGTGCC ACTCGGCCAT
    GTGATCAACA AGAGAAATAG CCCACAACCT GAGCTGTACG AGAATTGGCT GCTGTCAGGG
    AAACAGGGAG ACATCTTTCT GGGTGACCGG GTATCAGATT GCCAACGCAT TGAGCTCAAG
    CAGGCTATAC CTTCGTTGTC ACCCTCAGGC TGGATTCATG CTGTGTATAC TCCTACAGAC
    ACCTTGGTGT TTGGAGGCAA TTTTTTGCAT AGCTTCAATA TCCCCATGCA GTTAAAGATA
    TACAACATTG AAGATCGAAC ACGGGGAGCC GCCCGCAGCG GGGCGGCTCT CACGGTGTGG
    GCTGGGGCAG AGGCCGCCGC AGTTGTGGAA GTAGCTGAGA TGAACAGACG GATGCCTAGG
    GGAGGGGCAG CGGGGATAGG TGAGGCTGGC CGGCGGTGGG GAGAGAACGC AACTCCAGAG
    GCGCTCGTCT CCGGGCCGGG CTCTGCTGCA GAGGCTGAAC TTCAGGCGAC TCGCTGTCTG
    CCCGGGCACG GGGAGAGCCC TGTGCGCAGC CCGGGGGAGC GCGGCCGTGA CAGGGAGGTG
    ACCCTGAGGG CCGGGGTGTG GACCGCGACT GGGATTTTTA CTAGAGGCAG AACACCTAAC
    TCCTTCAGCA CAACTTCCAA TACAGAAAGC AAAACAAAGA CGCTGGGGAA ACTTCATCCA
    AGAGCCTCGG CAGCTCCAGA GGACGGGAAA CACAAGGTTT TGCTGGAGGA ACTTGCCAAC
    AGTGATCCCA AGTTAGCCCT CACTGGAGTC CCTATAGTAC AGTGGCCGAA AAGGGATAAG
    CTTAAATTCC CAACCCGGCC AAAGGTGCGG GTTCCTACTA TCCCCATCAC AAAGCCTCAC
    ACTATGAAAC CAGCGCCGAG GTTAACACCT GTGCGCCCAG CCGCTGCTTC TCCCATCGTG
    TCTGGAGCCA GACGGAGACG AGTGCGATGT CGAAAATGCA AAGCCTGCGT GCAGGGAGAG
    TGTGGTGTTT GTCACTACTG CAGGGACATG AAGAAGTTTG GGGGGCCTGG TCGCATGAAG
    CAGTCCTGTG TTCTCCGGCA GTGCTTGGCA CCCAGACTGC CTCACTCTGT CACATGTTCC
    CTCTGTGGAG AGGTGGATCA AAATGAGGAG ACACAGGACT TTGAGAAGAA ACTCATGGAA
    TGCTGTATCT GCAATGAGAT TGTTCATCCT GGCTGCCTCC AGATGGATGG AGAGGGGTTG
    CTTAATGAGG AATTGCCAAA TTGCTGGGAG TGTCCAAAGT GCTACCAAGA GGACAGCTCA
    GAAAAAGCCC AGAAGCGGAA AATGGAAGAG AGTGACGAAG AAGCCGTGCA AGCCAAAGTC
    CTCCGGCCCC TGCGGAGCTG CGACGAGCCC CTCACGCCCC CACCTCACTC GCCCACCTCC
    ATGCTGCAGC TCATCCACGA CCCGGCCTCC CCCCGGGGCG TGGTGACTCG GTCATCCCCT
    GGGGCCGGCC CCAGCGACCA CCACAGTGCC AGCCGCGATG AGCGCTTCAA ACGGCGGCAG
    TTGCTGCGGC TGCAGGCCAC AGAGCGCACC ATGGTACGGG AAAAGGAGAA CAATCCCAGC
    GGCAAAAAGG AGCTGTCTGA AGTTGAGAAA GCCAAGATCC GGGGATCGTA CCTCACTGTC
    ACGCTGCAGA GGCCCACCAA AGAGCTCCAC GGGACATCCA TTGTGCCCAA GCTGCAGGCC
    ATCACGGCCT CCTCTGCCAA CCTTCGCCAT TCCCCCCGTG TGCTGGTCCA GCACTGCCCA
    GCCCGAACCC CCCAGCGCGG GGATGAGGAG GGGCTGGGGG GAGAGGAGGA GGACGAGGAG
    GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CGGCCAGGCT GAATGGCCGG
    GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC
    TTCCGCTACC TCAGCCGCAG AGAACTTTGT GAATGTATGC GAGTGTGCAA GACGTGGTAT
    AAATGGTGCT GCGACAAGAG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCTATT
    GTACCCCAGG CTCTCAGTGG CATCATCAAG AGGCAGCCAG TTAGCCTTGA CCTCAGTTGG
    ACCAACATCT CCAAAAAACA GCTGACATGG CTCGTCAATA GGCTGCCAGG ACTGAAAGAC
    CTCCTCCTAG CAGGCTGCTC CTGGTCTGCA GTCTCTGCCC TCAGCACCTC CAGCTGCCCC
    CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATCA AGGACCCTCA AATTCGGGAC
    TTGCTGACTC CACCGGCGGA TAAACCGGGC CAGGACAATC GCAGCAAGCT CCGGAACATG
    ACCGACTTCC GGCTGGCAGG CCTTGACATC ACAGACGCCA CGCTTCGTCT CATCATTCGC
    CACATGCCCC TCCTGTCTCG ACTCGACCTC AGTCACTGCA GCCACCTGAC AGACCAGTCC
    TCCAATCTGC TCACTGCTGT TGGGTCTTCC ACTCGTTACT CCCTCACAGA GCTCAACATG
    GCAGGTTGCA ATAAACTGAC AGACCAGACC CTGATCTACC TACGGCGCAT CGCCAACGTC
    ACCTTGATCG ACCTTCGAGG ATGCAAGCAG ATCACTCGAA AAGCCTGCGA GCACTTCATC
    TCAGACTTGT CCATCAACAG TCTGTACTGC CTGTCTGACG AGAAGCTGAT ACAGAAGATC
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.