ZGRF1 cDNA ORF clone, Bos taurus(cattle)

The following ZGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235494 XM_024993502.1
Latest version!
Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa235491 XM_010805918.3
Latest version!
Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa235491 XM_024993499.1
Latest version!
Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa235492 XM_024993500.1
Latest version!
Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa235493 XM_024993501.1
Latest version!
Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa54374 XM_010805918.2
Latest version!
Bos taurus zinc finger, GRF-type containing 1 (ZGRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa54374 XM_010828055.2
Latest version!
Bos taurus zinc finger, GRF-type containing 1 (ZGRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235494
    Clone ID Related Accession (Same CDS sequence) XM_024993502.1
    Accession Version XM_024993502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ZGRF1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGAG
    GTAAAAGACT CTGAGCTATG CTTCCCAAGT GGGCAGAAAG TAAAATCTGC ATGTCTTCCC
    CAAAGGCAGA TTCACATACC AGCTGTTTTT CAGTCTCCTG CGCATTATAA GCAGGTTTTT
    ACATCTTGTC TCACAGAACA TTTAAATATA TTGCTATTTG GATTGGCACA AAGGTTGCAC
    AAAGCTCTTT CAAAAGTTGA CATATCATTT TACACATCAT CAAATGGAGA CAAACTGAAA
    AATGTAGAAA ATAATGTGCC ATCCTGCCAT CATCAGCAGC CTGCAAAACT GGTCATGGTT
    AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACCT GTGATGGACC CAAAGCTGAC
    CAATGTAAAT TTTTTAAGTG GTTTGAAGAA ATGACCCCAG GATATTTAAC ACAGGAAAAA
    TCTCTACCTA GCATGGTTTT AAGTGATATA AAAAGTATTG GCTTCTACTT AAGAAGTCAA
    AAGATACCCC TCTATGAGGA ATGCCAACTT TTCGTGAGGA AAGGATTTGA TTTTCAAAGA
    AAACGGTATG GTAAACTAAA GAAGTTTACT ACTGTAGATC CTGAATTTTA TAATGAACCT
    AAGAGCAAAC TTTATCTTAA ACTGAGTCGG AAAGAAAGTT CATCTGCTTA CAGCAAAGAT
    GATCTTTGGG TGGTTTCGAA AACCCTAGAC TTTGAATTGG ATACTTTTAT TGCATGCAGT
    ACTTTCTTTG GACCATCATC TATCAATGAG GTAGAGCTTC TACCTTTGAA AGGTTATTTC
    CCTTCGAACT GGCCCACTAA CACAGTGGTC CACGCCTTAT TGGTTTGTAA TGCCAGCACA
    GAGCTGACCA CTTTAAAAAA TATTCGGGAC TGCTTTAATC CAGCTACTCT ACCTCTAACA
    CAGTACCTCT TAACAATGTC TTCATCAACT ACAACGAGCA ACAAGAGAGT CAATAAGAGA
    AAGTTTATCC CACCAGCCTT CTCAAATATC AACACAAAAT TTGAACTACT CAGTATAGAA
    GCAACATTGA AGTTGGCTAG TGAGTTAATT CAGGTGCACA AGTTAAACAA GGATCAAGCC
    GCAGCTCTAA TTCAGATAGC TCAAATGATG GCATCACGCC AGAGTGTCGA AGAAGCGAAG
    GAACTGGCAA TTCATACCCT CCCTATCACG ATCATACACG GCGTTTTCGG AGCAGGAAAG
    AGTTACTTGC TAGCTGTGGT GATTTTGTTT TTTGTGCAAC TATTTGAAAA ATGTGAAGCT
    CTTACAGTTG GAAATGCAAG ACCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTAGCT
    GTTGACAGAG TGCTTCTTGG GCTTCTTAGT CTTGGATTTG AAAAGTTTGT CAGAGTTGGG
    AGTGTCAGAA AGATTGCCAA GCCAGTTTTA CCTTACAGTT TGTATGCTGG CTCAGAAAAT
    GAAAATGAGC AATTAAAGGA ACTGCATGCA CTCATGAAGG AAGACTTGAC TCCTGTGGAA
    AGAGTCTACG TGAGGAAAAG TATTGAGCAG CATAAACTGG GGACCAATAA AACCCTGCTG
    AAACAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT
    CTCAAATTCC CTGTGGTTGT CCTGGATGAG TGTAGTCAAA TGACAGAACC GGCTTCTCTC
    CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATACTTG TTGGAGATCC CAAACAGCTT
    CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GACTGGAACA AACTCTTTTT
    GACCGACTCT GCTTAATGGG CCACAAGCCA ATTCTGTTGA GAACTCAGTA CCGTTGTCAC
    CCCACAATCA GCGCTATTGC TAATGATCTG TTTTATGAAG GAAATCTCAT GAATGGCATT
    TCCGAAACAG AGAGGAGCCC TTTACTGGAA TGGCTCCCAA CTCTGTGTTT CTATAACGTA
    AAAGGACTGG AACAGATTGA AAGAGGTAAC AGCTTTCATA ATGTGGCAGA AGCTGCTTTT
    ACACTCAAGT TGATTCAATC ACTGATTGCG AGTGGAATAG CGGGCTCCAT GATTGGGGTG
    ATAACATTAT ACAAATCCCA GATGTACAAG CTCTGTCATC TACTCAGTGC GATGGACTTC
    GACCATCCCA ACTTGAAAGC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA
    AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACCAGACAAG TAGGATTTAT TGATTCAGAA
    AAAAGAATGA ATGTTGCATT GACCAGAGGA AGGAGGCATT TGTTGATTGT AGGAAATTTA
    GCCTGTCTGA GGAAAAATCG ACTATGGGGG CGTGTGATCC AACACTGTGA AGGGAGGGAA
    GGTGGATTGC AACATGCAAG CCAGTATGAA CCACAACTGG ACCATCTCCT TAAAGATTAT
    CTTGAAAAAC AAGCAGAAGA AAAACAGAAG AAAAAGGCTG AAAAAGATAA ATTGACAGAT
    AAAAAATCTT ATTCACAAAA AGACATGGTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849270.1
    CDS256..6168
    Translation

    Target ORF information:

    RefSeq Version XM_024993502.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGAG
    GTAAAAGACT CTGAGCTATG CTTCCCAAGT GGGCAGAAAG TAAAATCTGC ATGTCTTCCC
    CAAAGGCAGA TTCACATACC AGCTGTTTTT CAGTCTCCTG CGCATTATAA GCAGGTTTTT
    ACATCTTGTC TCACAGAACA TTTAAATATA TTGCTATTTG GATTGGCACA AAGGTTGCAC
    AAAGCTCTTT CAAAAGTTGA CATATCATTT TACACATCAT CAAATGGAGA CAAACTGAAA
    AATGTAGAAA ATAATGTGCC ATCCTGCCAT CATCAGCAGC CTGCAAAACT GGTCATGGTT
    AAAAAGGAAG GTCCAAATAA GGGTCGTCTC TTTTATACCT GTGATGGACC CAAAGCTGAC
    CAATGTAAAT TTTTTAAGTG GTTTGAAGAA ATGACCCCAG GATATTTAAC ACAGGAAAAA
    TCTCTACCTA GCATGGTTTT AAGTGATATA AAAAGTATTG GCTTCTACTT AAGAAGTCAA
    AAGATACCCC TCTATGAGGA ATGCCAACTT TTCGTGAGGA AAGGATTTGA TTTTCAAAGA
    AAACGGTATG GTAAACTAAA GAAGTTTACT ACTGTAGATC CTGAATTTTA TAATGAACCT
    AAGAGCAAAC TTTATCTTAA ACTGAGTCGG AAAGAAAGTT CATCTGCTTA CAGCAAAGAT
    GATCTTTGGG TGGTTTCGAA AACCCTAGAC TTTGAATTGG ATACTTTTAT TGCATGCAGT
    ACTTTCTTTG GACCATCATC TATCAATGAG GTAGAGCTTC TACCTTTGAA AGGTTATTTC
    CCTTCGAACT GGCCCACTAA CACAGTGGTC CACGCCTTAT TGGTTTGTAA TGCCAGCACA
    GAGCTGACCA CTTTAAAAAA TATTCGGGAC TGCTTTAATC CAGCTACTCT ACCTCTAACA
    CAGTACCTCT TAACAATGTC TTCATCAACT ACAACGAGCA ACAAGAGAGT CAATAAGAGA
    AAGTTTATCC CACCAGCCTT CTCAAATATC AACACAAAAT TTGAACTACT CAGTATAGAA
    GCAACATTGA AGTTGGCTAG TGAGTTAATT CAGGTGCACA AGTTAAACAA GGATCAAGCC
    GCAGCTCTAA TTCAGATAGC TCAAATGATG GCATCACGCC AGAGTGTCGA AGAAGCGAAG
    GAACTGGCAA TTCATACCCT CCCTATCACG ATCATACACG GCGTTTTCGG AGCAGGAAAG
    AGTTACTTGC TAGCTGTGGT GATTTTGTTT TTTGTGCAAC TATTTGAAAA ATGTGAAGCT
    CTTACAGTTG GAAATGCAAG ACCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTAGCT
    GTTGACAGAG TGCTTCTTGG GCTTCTTAGT CTTGGATTTG AAAAGTTTGT CAGAGTTGGG
    AGTGTCAGAA AGATTGCCAA GCCAGTTTTA CCTTACAGTT TGTATGCTGG CTCAGAAAAT
    GAAAATGAGC AATTAAAGGA ACTGCATGCA CTCATGAAGG AAGACTTGAC TCCTGTGGAA
    AGAGTCTACG TGAGGAAAAG TATTGAGCAG CATAAACTGG GGACCAATAA AACCCTGCTG
    AAACAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT
    CTCAAATTCC CTGTGGTTGT CCTGGATGAG TGTAGTCAAA TGACAGAACC GGCTTCTCTC
    CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATACTTG TTGGAGATCC CAAACAGCTT
    CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GACTGGAACA AACTCTTTTT
    GACCGACTCT GCTTAATGGG CCACAAGCCA ATTCTGTTGA GAACTCAGTA CCGTTGTCAC
    CCCACAATCA GCGCTATTGC TAATGATCTG TTTTATGAAG GAAATCTCAT GAATGGCATT
    TCCGAAACAG AGAGGAGCCC TTTACTGGAA TGGCTCCCAA CTCTGTGTTT CTATAACGTA
    AAAGGACTGG AACAGATTGA AAGAGGTAAC AGCTTTCATA ATGTGGCAGA AGCTGCTTTT
    ACACTCAAGT TGATTCAATC ACTGATTGCG AGTGGAATAG CGGGCTCCAT GATTGGGGTG
    ATAACATTAT ACAAATCCCA GATGTACAAG CTCTGTCATC TACTCAGTGC GATGGACTTC
    GACCATCCCA ACTTGAAAGC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA
    AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACCAGACAAG TAGGATTTAT TGATTCAGAA
    AAAAGAATGA ATGTTGCATT GACCAGAGGA AGGAGGCATT TGTTGATTGT AGGAAATTTA
    GCCTGTCTGA GGAAAAATCG ACTATGGGGG CGTGTGATCC AACACTGTGA AGGGAGGGAA
    GGTGGATTGC AACATGCAAG CCAGTATGAA CCACAACTGG ACCATCTCCT TAAAGATTAT
    CTTGAAAAAC AAGCAGAAGA AAAACAGAAG AAAAAGGCTG AAAAAGATAA ATTGACAGAT
    AAAAAATCTT ATTCACAAAA AGACATGGTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235491
    Clone ID Related Accession (Same CDS sequence) XM_024993499.1 , XM_010805918.3
    Accession Version XM_024993499.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ZGRF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849267.1
    CDS233..6748
    Translation

    Target ORF information:

    RefSeq Version XM_024993499.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993499.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235491
    Clone ID Related Accession (Same CDS sequence) XM_024993499.1 , XM_010805918.3
    Accession Version XM_010805918.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ZGRF1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805918.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804220.2
    CDS257..6772
    Translation

    Target ORF information:

    RefSeq Version XM_010805918.3
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805918.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235492
    Clone ID Related Accession (Same CDS sequence) XM_024993500.1
    Accession Version XM_024993500.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ZGRF1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACATGGTC CACGCCTTAT TGGTTTGTAA TGCCAGCACA
    GAGCTGACCA CTTTAAAAAA TATTCGGGAC TGCTTTAATC CAGCTACTCT ACCTCTAACA
    CAGTACCTCT TAACAATGTC TTCATCAACT ACAACGAGCA ACAAGAGAGT CAATAAGAGA
    AAGTTTATCC CACCAGCCTT CTCAAATATC AACACAAAAT TTGAACTACT CAGTATAGAA
    GCAACATTGA AGTTGGCTAG TGAGTTAATT CAGGTGCACA AGTTAAACAA GGATCAAGCC
    GCAGCTCTAA TTCAGATAGC TCAAATGATG GCATCACGCC AGAGTGTCGA AGAAGCGAAG
    GAACTGGCAA TTCATACCCT CCCTATCACG ATCATACACG GCGTTTTCGG AGCAGGAAAG
    AGTTACTTGC TAGCTGTGGT GATTTTGTTT TTTGTGCAAC TATTTGAAAA ATGTGAAGCT
    CTTACAGTTG GAAATGCAAG ACCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTAGCT
    GTTGACAGAG TGCTTCTTGG GCTTCTTAGT CTTGGATTTG AAAAGTTTGT CAGAGTTGGG
    AGTGTCAGAA AGATTGCCAA GCCAGTTTTA CCTTACAGTT TGTATGCTGG CTCAGAAAAT
    GAAAATGAGC AATTAAAGGA ACTGCATGCA CTCATGAAGG AAGACTTGAC TCCTGTGGAA
    AGAGTCTACG TGAGGAAAAG TATTGAGCAG CATAAACTGG GGACCAATAA AACCCTGCTG
    AAACAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT
    CTCAAATTCC CTGTGGTTGT CCTGGATGAG TGTAGTCAAA TGACAGAACC GGCTTCTCTC
    CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATACTTG TTGGAGATCC CAAACAGCTT
    CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GACTGGAACA AACTCTTTTT
    GACCGACTCT GCTTAATGGG CCACAAGCCA ATTCTGTTGA GAACTCAGTA CCGTTGTCAC
    CCCACAATCA GCGCTATTGC TAATGATCTG TTTTATGAAG GAAATCTCAT GAATGGCATT
    TCCGAAACAG AGAGGAGCCC TTTACTGGAA TGGCTCCCAA CTCTGTGTTT CTATAACGTA
    AAAGGACTGG AACAGATTGA AAGAGGTAAC AGCTTTCATA ATGTGGCAGA AGCTGCTTTT
    ACACTCAAGT TGATTCAATC ACTGATTGCG AGTGGAATAG CGGGCTCCAT GATTGGGGTG
    ATAACATTAT ACAAATCCCA GATGTACAAG CTCTGTCATC TACTCAGTGC GATGGACTTC
    GACCATCCCA ACTTGAAAGC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA
    AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACCAGACAAG TAGGATTTAT TGATTCAGAA
    AAAAGAATGA ATGTTGCATT GACCAGAGGA AGGAGGCATT TGTTGATTGT AGGAAATTTA
    GCCTGTCTGA GGAAAAATCG ACTATGGGGG CGTGTGATCC AACACTGTGA AGGGAGGGAA
    GGTGGATTGC AACATGCAAG CCAGTATGAA CCACAACTGG ACCATCTCCT TAAAGATTAT
    CTTGAAAAAC AAGCAGAAGA AAAACAGAAG AAAAAGGCTG AAAAAGATAA ATTGACAGAT
    AAAAAATCTT ATTCACAAAA AGACATGGTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849268.1
    CDS257..6769
    Translation

    Target ORF information:

    RefSeq Version XM_024993500.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993500.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACATGGTC CACGCCTTAT TGGTTTGTAA TGCCAGCACA
    GAGCTGACCA CTTTAAAAAA TATTCGGGAC TGCTTTAATC CAGCTACTCT ACCTCTAACA
    CAGTACCTCT TAACAATGTC TTCATCAACT ACAACGAGCA ACAAGAGAGT CAATAAGAGA
    AAGTTTATCC CACCAGCCTT CTCAAATATC AACACAAAAT TTGAACTACT CAGTATAGAA
    GCAACATTGA AGTTGGCTAG TGAGTTAATT CAGGTGCACA AGTTAAACAA GGATCAAGCC
    GCAGCTCTAA TTCAGATAGC TCAAATGATG GCATCACGCC AGAGTGTCGA AGAAGCGAAG
    GAACTGGCAA TTCATACCCT CCCTATCACG ATCATACACG GCGTTTTCGG AGCAGGAAAG
    AGTTACTTGC TAGCTGTGGT GATTTTGTTT TTTGTGCAAC TATTTGAAAA ATGTGAAGCT
    CTTACAGTTG GAAATGCAAG ACCGTGGAAA CTTCTGATTT CTTCTTCTAC TAATGTAGCT
    GTTGACAGAG TGCTTCTTGG GCTTCTTAGT CTTGGATTTG AAAAGTTTGT CAGAGTTGGG
    AGTGTCAGAA AGATTGCCAA GCCAGTTTTA CCTTACAGTT TGTATGCTGG CTCAGAAAAT
    GAAAATGAGC AATTAAAGGA ACTGCATGCA CTCATGAAGG AAGACTTGAC TCCTGTGGAA
    AGAGTCTACG TGAGGAAAAG TATTGAGCAG CATAAACTGG GGACCAATAA AACCCTGCTG
    AAACAGGTTC GAGTAGTTGG AGTTACCTGT GCAGCCTGCC CATTCCCATG CATGAATGAT
    CTCAAATTCC CTGTGGTTGT CCTGGATGAG TGTAGTCAAA TGACAGAACC GGCTTCTCTC
    CTTCCCATTG CAAGGTTTGA GTGTGAAAAG CTGATACTTG TTGGAGATCC CAAACAGCTT
    CCTCCTACTA TTCAGGGTTC TGATGCAGCT CATGAAAATG GACTGGAACA AACTCTTTTT
    GACCGACTCT GCTTAATGGG CCACAAGCCA ATTCTGTTGA GAACTCAGTA CCGTTGTCAC
    CCCACAATCA GCGCTATTGC TAATGATCTG TTTTATGAAG GAAATCTCAT GAATGGCATT
    TCCGAAACAG AGAGGAGCCC TTTACTGGAA TGGCTCCCAA CTCTGTGTTT CTATAACGTA
    AAAGGACTGG AACAGATTGA AAGAGGTAAC AGCTTTCATA ATGTGGCAGA AGCTGCTTTT
    ACACTCAAGT TGATTCAATC ACTGATTGCG AGTGGAATAG CGGGCTCCAT GATTGGGGTG
    ATAACATTAT ACAAATCCCA GATGTACAAG CTCTGTCATC TACTCAGTGC GATGGACTTC
    GACCATCCCA ACTTGAAAGC TGTGCAGGTG TCCACAGTAG ATGCTTTTCA GGGAGCTGAA
    AAGGAGATCA TTATTCTGTC CTGTGTAAGG ACCAGACAAG TAGGATTTAT TGATTCAGAA
    AAAAGAATGA ATGTTGCATT GACCAGAGGA AGGAGGCATT TGTTGATTGT AGGAAATTTA
    GCCTGTCTGA GGAAAAATCG ACTATGGGGG CGTGTGATCC AACACTGTGA AGGGAGGGAA
    GGTGGATTGC AACATGCAAG CCAGTATGAA CCACAACTGG ACCATCTCCT TAAAGATTAT
    CTTGAAAAAC AAGCAGAAGA AAAACAGAAG AAAAAGGCTG AAAAAGATAA ATTGACAGAT
    AAAAAATCTT ATTCACAAAA AGACATGGTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235493
    Clone ID Related Accession (Same CDS sequence) XM_024993501.1
    Accession Version XM_024993501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6330bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein ZGRF1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG TTCCCAAACG AAGAAAACTT TACAAAAGGA
    GATACTAGCT TTACTATTGA AGACAATTTA CAAGTGAGAG CTGAGTTTAA TAAGGACTTG
    ACTAAAAATG ATTCAATTTT AGATTTCCCT CCTAAAGATT CAGACCATTT ATATAATTAT
    TTGGATTATA ATTTTAAATC AACAGAGAGG ACTTCGTGGG AAAAAAATAA GGTGACGTCA
    CCAGAACAGA AGATCTCAAC ATTAAGCCCT GTTTCTACTT TATCTTTTAA CTCGAGAGAT
    GATGACTTCA TGCTAGAATT CTCTGAGGAG TCCCTGAAAG GGCAAACTTT ACCTGTAATG
    AAAAAATCAA GTTCTCTGGA GAATAAGAAC CTTCAGAGGC TTTCCTTTGA AGTAAGTAGA
    ACCCGAGCTC CACTTGTTGC TTTGCCGCCT GCCAGTGGGC CACCTGACTC AGATTCTTGT
    TCACGTATGA CGGACCGTGA CAGGCAGAAG GCTTCTGGTC CTCCCATATT CAGCCTGCGT
    GAAGAGTCAG CAGTTCCTTC TTTCAGCTTT GGGCCCAAGG ACCAAAGCGA AGCTTCTTTC
    TCTAACGAAT CTAGGGAAGT GACTCCAAGT GAGGTTTCCC TTCTTGATAA TACATCTACT
    CAAAGCAAGT GGCTGAAATA TCAAAATACA CGCCAGTGCA ACTTGACTAC TCCAGACAGA
    GTTGATGAGA AAGTAACAGA CGGCTTCTTT GCTAAGGCTG TTTCTGGGAT GCGTTTTAGT
    AACACAGGTG AAAGAGAGAA TGATGCTGTG AATGAAAGTT CTTTAGATTC TGTGTATTTA
    CAAATGATGA AACACATGCT TCAACAGCAG CAGCAGGATT TTAGCAGTCA AGATTCGGTT
    TGCAGAAAGA AGGCATTTTC TTTGAATATA AACGAGGCTT CTAAGACTGA GGAAGTTCAG
    AATATGTTAG GAGAGTATGC CTGCTACAAC TGCAGTGTAA CAGGAGATTT ACAGGAGGTA
    AAAGACTCTG AGCTATGCTT CCCAAGTGGG CAGAAAGTAA AATCTGCATG TCTTCCCCAA
    AGGCAGATTC ACATACCAGC TGTTTTTCAG TCTCCTGCGC ATTATAAGCA GGTTTTTACA
    TCTTGTCTCA CAGAACATTT AAATATATTG CTATTTGGAT TGGCACAAAG GTTGCACAAA
    GCTCTTTCAA AAGTTGACAT ATCATTTTAC ACATCATCAA ATGGAGACAA ACTGAAAAAT
    GTAGAAAATA ATGTGCCATC CTGCCATCAT CAGCAGCCTG CAAAACTGGT CATGGTTAAA
    AAGGAAGGTC CAAATAAGGG TCGTCTCTTT TATACCTGTG ATGGACCCAA AGCTGACCAA
    TGTAAATTTT TTAAGTGGTT TGAAGAAATG ACCCCAGGAT ATTTAACACA GGAAAAATCT
    CTACCTAGCA TGGTTTTAAG TGATATAAAA AGTATTGGCT TCTACTTAAG AAGTCAAAAG
    ATACCCCTCT ATGAGGAATG CCAACTTTTC GTGAGGAAAG GATTTGATTT TCAAAGAAAA
    CGGTATGGTA AACTAAAGAA GTTTACTACT GTAGATCCTG AATTTTATAA TGAACCTAAG
    AGCAAACTTT ATCTTAAACT GAGTCGGAAA GAAAGTTCAT CTGCTTACAG CAAAGATGAT
    CTTTGGGTGG TTTCGAAAAC CCTAGACTTT GAATTGGATA CTTTTATTGC ATGCAGTACT
    TTCTTTGGAC CATCATCTAT CAATGAGGTA GAGCTTCTAC CTTTGAAAGG TTATTTCCCT
    TCGAACTGGC CCACTAACAC AGTGGTCCAC GCCTTATTGG TTTGTAATGC CAGCACAGAG
    CTGACCACTT TAAAAAATAT TCGGGACTGC TTTAATCCAG CTACTCTACC TCTAACACAG
    TACCTCTTAA CAATGTCTTC ATCAACTACA ACGAGCAACA AGAGAGTCAA TAAGAGAAAG
    TTTATCCCAC CAGCCTTCTC AAATATCAAC ACAAAATTTG AACTACTCAG TATAGAAGCA
    ACATTGAAGT TGGCTAGTGA GTTAATTCAG GTGCACAAGT TAAACAAGGA TCAAGCCGCA
    GCTCTAATTC AGATAGCTCA AATGATGGCA TCACGCCAGA GTGTCGAAGA AGCGAAGGAA
    CTGGCAATTC ATACCCTCCC TATCACGATC ATACACGGCG TTTTCGGAGC AGGAAAGAGT
    TACTTGCTAG CTGTGGTGAT TTTGTTTTTT GTGCAACTAT TTGAAAAATG TGAAGCTCTT
    ACAGTTGGAA ATGCAAGACC GTGGAAACTT CTGATTTCTT CTTCTACTAA TGTAGCTGTT
    GACAGAGTGC TTCTTGGGCT TCTTAGTCTT GGATTTGAAA AGTTTGTCAG AGTTGGGAGT
    GTCAGAAAGA TTGCCAAGCC AGTTTTACCT TACAGTTTGT ATGCTGGCTC AGAAAATGAA
    AATGAGCAAT TAAAGGAACT GCATGCACTC ATGAAGGAAG ACTTGACTCC TGTGGAAAGA
    GTCTACGTGA GGAAAAGTAT TGAGCAGCAT AAACTGGGGA CCAATAAAAC CCTGCTGAAA
    CAGGTTCGAG TAGTTGGAGT TACCTGTGCA GCCTGCCCAT TCCCATGCAT GAATGATCTC
    AAATTCCCTG TGGTTGTCCT GGATGAGTGT AGTCAAATGA CAGAACCGGC TTCTCTCCTT
    CCCATTGCAA GGTTTGAGTG TGAAAAGCTG ATACTTGTTG GAGATCCCAA ACAGCTTCCT
    CCTACTATTC AGGGTTCTGA TGCAGCTCAT GAAAATGGAC TGGAACAAAC TCTTTTTGAC
    CGACTCTGCT TAATGGGCCA CAAGCCAATT CTGTTGAGAA CTCAGTACCG TTGTCACCCC
    ACAATCAGCG CTATTGCTAA TGATCTGTTT TATGAAGGAA ATCTCATGAA TGGCATTTCC
    GAAACAGAGA GGAGCCCTTT ACTGGAATGG CTCCCAACTC TGTGTTTCTA TAACGTAAAA
    GGACTGGAAC AGATTGAAAG AGGTAACAGC TTTCATAATG TGGCAGAAGC TGCTTTTACA
    CTCAAGTTGA TTCAATCACT GATTGCGAGT GGAATAGCGG GCTCCATGAT TGGGGTGATA
    ACATTATACA AATCCCAGAT GTACAAGCTC TGTCATCTAC TCAGTGCGAT GGACTTCGAC
    CATCCCAACT TGAAAGCTGT GCAGGTGTCC ACAGTAGATG CTTTTCAGGG AGCTGAAAAG
    GAGATCATTA TTCTGTCCTG TGTAAGGACC AGACAAGTAG GATTTATTGA TTCAGAAAAA
    AGAATGAATG TTGCATTGAC CAGAGGAAGG AGGCATTTGT TGATTGTAGG AAATTTAGCC
    TGTCTGAGGA AAAATCGACT ATGGGGGCGT GTGATCCAAC ACTGTGAAGG GAGGGAAGGT
    GGATTGCAAC ATGCAAGCCA GTATGAACCA CAACTGGACC ATCTCCTTAA AGATTATCTT
    GAAAAACAAG CAGAAGAAAA ACAGAAGAAA AAGGCTGAAA AAGATAAATT GACAGATAAA
    AAATCTTATT CACAAAAAGA CATGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024849269.1
    CDS257..6586
    Translation

    Target ORF information:

    RefSeq Version XM_024993501.1
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger GRF-type containing 1 (ZGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACATCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGAGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAGGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG TTCCCAAACG AAGAAAACTT TACAAAAGGA
    GATACTAGCT TTACTATTGA AGACAATTTA CAAGTGAGAG CTGAGTTTAA TAAGGACTTG
    ACTAAAAATG ATTCAATTTT AGATTTCCCT CCTAAAGATT CAGACCATTT ATATAATTAT
    TTGGATTATA ATTTTAAATC AACAGAGAGG ACTTCGTGGG AAAAAAATAA GGTGACGTCA
    CCAGAACAGA AGATCTCAAC ATTAAGCCCT GTTTCTACTT TATCTTTTAA CTCGAGAGAT
    GATGACTTCA TGCTAGAATT CTCTGAGGAG TCCCTGAAAG GGCAAACTTT ACCTGTAATG
    AAAAAATCAA GTTCTCTGGA GAATAAGAAC CTTCAGAGGC TTTCCTTTGA AGTAAGTAGA
    ACCCGAGCTC CACTTGTTGC TTTGCCGCCT GCCAGTGGGC CACCTGACTC AGATTCTTGT
    TCACGTATGA CGGACCGTGA CAGGCAGAAG GCTTCTGGTC CTCCCATATT CAGCCTGCGT
    GAAGAGTCAG CAGTTCCTTC TTTCAGCTTT GGGCCCAAGG ACCAAAGCGA AGCTTCTTTC
    TCTAACGAAT CTAGGGAAGT GACTCCAAGT GAGGTTTCCC TTCTTGATAA TACATCTACT
    CAAAGCAAGT GGCTGAAATA TCAAAATACA CGCCAGTGCA ACTTGACTAC TCCAGACAGA
    GTTGATGAGA AAGTAACAGA CGGCTTCTTT GCTAAGGCTG TTTCTGGGAT GCGTTTTAGT
    AACACAGGTG AAAGAGAGAA TGATGCTGTG AATGAAAGTT CTTTAGATTC TGTGTATTTA
    CAAATGATGA AACACATGCT TCAACAGCAG CAGCAGGATT TTAGCAGTCA AGATTCGGTT
    TGCAGAAAGA AGGCATTTTC TTTGAATATA AACGAGGCTT CTAAGACTGA GGAAGTTCAG
    AATATGTTAG GAGAGTATGC CTGCTACAAC TGCAGTGTAA CAGGAGATTT ACAGGAGGTA
    AAAGACTCTG AGCTATGCTT CCCAAGTGGG CAGAAAGTAA AATCTGCATG TCTTCCCCAA
    AGGCAGATTC ACATACCAGC TGTTTTTCAG TCTCCTGCGC ATTATAAGCA GGTTTTTACA
    TCTTGTCTCA CAGAACATTT AAATATATTG CTATTTGGAT TGGCACAAAG GTTGCACAAA
    GCTCTTTCAA AAGTTGACAT ATCATTTTAC ACATCATCAA ATGGAGACAA ACTGAAAAAT
    GTAGAAAATA ATGTGCCATC CTGCCATCAT CAGCAGCCTG CAAAACTGGT CATGGTTAAA
    AAGGAAGGTC CAAATAAGGG TCGTCTCTTT TATACCTGTG ATGGACCCAA AGCTGACCAA
    TGTAAATTTT TTAAGTGGTT TGAAGAAATG ACCCCAGGAT ATTTAACACA GGAAAAATCT
    CTACCTAGCA TGGTTTTAAG TGATATAAAA AGTATTGGCT TCTACTTAAG AAGTCAAAAG
    ATACCCCTCT ATGAGGAATG CCAACTTTTC GTGAGGAAAG GATTTGATTT TCAAAGAAAA
    CGGTATGGTA AACTAAAGAA GTTTACTACT GTAGATCCTG AATTTTATAA TGAACCTAAG
    AGCAAACTTT ATCTTAAACT GAGTCGGAAA GAAAGTTCAT CTGCTTACAG CAAAGATGAT
    CTTTGGGTGG TTTCGAAAAC CCTAGACTTT GAATTGGATA CTTTTATTGC ATGCAGTACT
    TTCTTTGGAC CATCATCTAT CAATGAGGTA GAGCTTCTAC CTTTGAAAGG TTATTTCCCT
    TCGAACTGGC CCACTAACAC AGTGGTCCAC GCCTTATTGG TTTGTAATGC CAGCACAGAG
    CTGACCACTT TAAAAAATAT TCGGGACTGC TTTAATCCAG CTACTCTACC TCTAACACAG
    TACCTCTTAA CAATGTCTTC ATCAACTACA ACGAGCAACA AGAGAGTCAA TAAGAGAAAG
    TTTATCCCAC CAGCCTTCTC AAATATCAAC ACAAAATTTG AACTACTCAG TATAGAAGCA
    ACATTGAAGT TGGCTAGTGA GTTAATTCAG GTGCACAAGT TAAACAAGGA TCAAGCCGCA
    GCTCTAATTC AGATAGCTCA AATGATGGCA TCACGCCAGA GTGTCGAAGA AGCGAAGGAA
    CTGGCAATTC ATACCCTCCC TATCACGATC ATACACGGCG TTTTCGGAGC AGGAAAGAGT
    TACTTGCTAG CTGTGGTGAT TTTGTTTTTT GTGCAACTAT TTGAAAAATG TGAAGCTCTT
    ACAGTTGGAA ATGCAAGACC GTGGAAACTT CTGATTTCTT CTTCTACTAA TGTAGCTGTT
    GACAGAGTGC TTCTTGGGCT TCTTAGTCTT GGATTTGAAA AGTTTGTCAG AGTTGGGAGT
    GTCAGAAAGA TTGCCAAGCC AGTTTTACCT TACAGTTTGT ATGCTGGCTC AGAAAATGAA
    AATGAGCAAT TAAAGGAACT GCATGCACTC ATGAAGGAAG ACTTGACTCC TGTGGAAAGA
    GTCTACGTGA GGAAAAGTAT TGAGCAGCAT AAACTGGGGA CCAATAAAAC CCTGCTGAAA
    CAGGTTCGAG TAGTTGGAGT TACCTGTGCA GCCTGCCCAT TCCCATGCAT GAATGATCTC
    AAATTCCCTG TGGTTGTCCT GGATGAGTGT AGTCAAATGA CAGAACCGGC TTCTCTCCTT
    CCCATTGCAA GGTTTGAGTG TGAAAAGCTG ATACTTGTTG GAGATCCCAA ACAGCTTCCT
    CCTACTATTC AGGGTTCTGA TGCAGCTCAT GAAAATGGAC TGGAACAAAC TCTTTTTGAC
    CGACTCTGCT TAATGGGCCA CAAGCCAATT CTGTTGAGAA CTCAGTACCG TTGTCACCCC
    ACAATCAGCG CTATTGCTAA TGATCTGTTT TATGAAGGAA ATCTCATGAA TGGCATTTCC
    GAAACAGAGA GGAGCCCTTT ACTGGAATGG CTCCCAACTC TGTGTTTCTA TAACGTAAAA
    GGACTGGAAC AGATTGAAAG AGGTAACAGC TTTCATAATG TGGCAGAAGC TGCTTTTACA
    CTCAAGTTGA TTCAATCACT GATTGCGAGT GGAATAGCGG GCTCCATGAT TGGGGTGATA
    ACATTATACA AATCCCAGAT GTACAAGCTC TGTCATCTAC TCAGTGCGAT GGACTTCGAC
    CATCCCAACT TGAAAGCTGT GCAGGTGTCC ACAGTAGATG CTTTTCAGGG AGCTGAAAAG
    GAGATCATTA TTCTGTCCTG TGTAAGGACC AGACAAGTAG GATTTATTGA TTCAGAAAAA
    AGAATGAATG TTGCATTGAC CAGAGGAAGG AGGCATTTGT TGATTGTAGG AAATTTAGCC
    TGTCTGAGGA AAAATCGACT ATGGGGGCGT GTGATCCAAC ACTGTGAAGG GAGGGAAGGT
    GGATTGCAAC ATGCAAGCCA GTATGAACCA CAACTGGACC ATCTCCTTAA AGATTATCTT
    GAAAAACAAG CAGAAGAAAA ACAGAAGAAA AAGGCTGAAA AAGATAAATT GACAGATAAA
    AAATCTTATT CACAAAAAGA CATGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54374
    Clone ID Related Accession (Same CDS sequence) XM_010805918.2 , XM_010828055.2
    Accession Version XM_010805918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ZGRF1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103961.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACGTCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGCGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAAGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804220.1
    CDS257..6772
    Translation

    Target ORF information:

    RefSeq Version XM_010805918.2
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger, GRF-type containing 1 (ZGRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACGTCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGCGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAAGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54374
    Clone ID Related Accession (Same CDS sequence) XM_010805918.2 , XM_010828055.2
    Accession Version XM_010828055.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6516bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein ZGRF1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010828055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACGTCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGCGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAAGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010826357.2
    CDS257..6772
    Translation

    Target ORF information:

    RefSeq Version XM_010828055.2
    Organism Bos taurus(cattle)
    Definition Bos taurus zinc finger, GRF-type containing 1 (ZGRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010828055.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGACTACTG ATTATTTTCT GGACCTGACT GTAATATCAG AAGAAATAAT GGAAAGCCAA 
    GAATTTATTG TACTATATAC TCATCAAAAG ACGAAGAAGT CAAAAGTGTG GCAAGATGGA
    ATTCTGAAGA TCACTCACTT AGGAAACAAA GCAATTTTAT ATGATGACAA AGGAGGATGT
    TTGGAGAGTT TATTTCTTAA GTGCCTTGAG GTTAAACCTG GAGATGATTT AGAAAGTGAT
    CGGTACTTAA TCACAGTTGA GGAGGTTAAA GTTGCTGGAA GCACAGCTGT CAAGCAGGAT
    GTCATTAAAG AAGCACCAGC ATTAAATTCA AAGAGGTTTA TGTCTTCTGG CCGATCCCTT
    GGGTGTCAGC CTGCTGGTTT AAAAAGGAAG TTCACTGGTT TTCGAGGACC ACGTCAAGTG
    CCAAAGAAAA TGGTTATTAC AGAAAATAGT GACCCAGCTG CTTCACTTGA GTCTGAGAAA
    CCTGGTCCTG CCTTTCTTTC TCCATTGTAC AGCACACCTC CTTTGTTTCC TACAATTGGC
    AAGAAAGATG CAAGTAATAT GCCAACAGAC TCTGAGAACA TTGTCACTTA CAAGAACAGA
    GAACGAAATG GCATACCTTT TTCTTCAGTT ATCTCTACTC CATCCTTCAA GATTAACCCA
    GAAGTGCTAT GTGAAGAAAA TTATGTTTGT TCTCCTGTCA GTTCTGTAAA TAAGCATTCA
    GATTCTTTAC TGACTGATGA GTCCATGAAA AGAGACAGTT TGGTATCTCA CAGTTTAGGA
    GTTTCACAAA ACATTAGAAG CAAAGCCCAG ATATTAGCTC TTCTGAAGTC CACATCCACA
    AGTACGTCTA AGGAGTTAAA TTCTGAGATT ATAGGCCATT TCCCTCAGAT ACAACCACAA
    GGAAGTTTAG AAATTCCTGC TGAACCAAAT TGCTTACCTG AACAGGAAGA GTATGCTGAA
    GTGAAGCGCA CAGAAAGTTT ACACAACCAG CATCAATCAG AAAATACCAT GAGAAATAAA
    AGCCGGTGGG CCGTGTATTT ATCCTCACAG AGTTCACCTG TACATTCTTC TACAGTAGAT
    GGAAAAGATA TAGAAAAGAA ACCCAAGACC CAAGAAGGTC ATTTAAACTT GAAAGACCTT
    TCGATGCAAA ACAGGATCCA GTTCTTGGAA ACATGTGCTG AAAAGGGAAA AAGGTATAAT
    GAAGATAAGT CAATAGATGA TAATGATCAA TCCTGGGATC AAGAAGGAAA ACTAGAAATT
    CCTTCATTCT GTGAAAACAG TAGCTTACCA ATTAATTGTA ACATTGTAGT AAATGATGGC
    TTATTATTAG ATTCTGACAT TCAAAAAAAT AATAAAAGAT CTGTTGATCA AAATGACCAG
    ATAGGCAAAA AAGGATCAAT TCTTCTTAGA GAAAAAGCAC AGGAGATAAA TATAAATGGA
    ACACCAGAAA AGAAGTATGA ACATCTACAA ATTGAATCTT CACTAAGTAA CAATTCTGGG
    ATCTCTGATG ATATATTTTC TAAAAGTAGT ACTAACAATG AAAGCCTTAG TAGTATTCAT
    GAACCCATGA GTGATATATC ACAGCCATTT GTGGAAGTCA CATTTAATCT AAACAATTTT
    GAAACCAGTG ACACTGAAGA GGAATCACAG GAAAGCAGCA AAACTTCCCA GGATTCAGAG
    GATTCAACTT TGGTTAATAG CAGCAATTCA AGATGTGAGA ATATAAGCTG TAAAGAAGTT
    GGCAGTGAAC ATCTGCCTCT CTTAACGTCT ACTGGGGGCA AACTTTCAGA GACATTTCCC
    ATGAAAGAGA TTCTGCCTTC ACAGTTTTGT GATAAAACTT GTGTAGGTTA TGACACAGAA
    ACTTGGAAAG CTGGAAACAC TGGAAAAGAA AGAAAGGAGG TGTGTAGTGA CACATTAAGC
    AATTTTGACT CATCTTTAGA GTGGACTGAT GATAGATATG TAGAAAATGA TGAAGGCGCT
    AATAAATCCA TTAGTGCTTC CTTCCCAAGT AAATCTGAAA GTATGAACAC AAATTTACAT
    ATTCCTCATC TTTTAAACAT AGCCACTGAT CAGAAACCTG AAAACAACCT ATTTTCAGAA
    CAGGCTCAGA CTGTTCTGGG AAGTGACTTA GACAAAACAA CCAATCAGGT TTTACCGTTA
    ACCTCTAGCA GTGGCAACAG TATCCAGATA TTAAATGCCA AACAGATTCA CTCCAAAGAA
    TGTACTGCAC TTGATAAATC ACAGACCCAA GGTTCCAATT CTTTGTGTTA CCCTTTGGGA
    AAAACACATA TTTCCAAAGA CACAGAATCA CATATTTCTG AAGCTGAAGA GGTGGGAAGG
    AGTCGAGGTT TATCCCAGGA CCATATTGAT GTAGAAACTT CTGGAGAAAA CAAACAATAT
    GGGAATAATC TTAGAAATTC TTCAGAAATT TCTGGATTAG TAAATAGCAT TTCTCTCTTA
    AAATCATTGA CTGAACACAG TACGGCTTTG GAAGGCTTGG AAGTATTGAA AAAGAAAAAT
    ACCTCCTTGA AACAACAAGG AACTCTGCAG ACATATGAGC CAGATAGCAG TCCTGAAGTT
    GGGAAACCAT TGACTACAAT AGTTTCACAA GCTATACCAA AGTCTCCTCA TCTGAACCAG
    GATCCTCATC AGATGATAAA AGAAAATGAA GCTGAACCAA GTGAATCACG TCAGTCTTTG
    CAGTTCATTC CCTTGGGCAC TGAAGAAGAG ACTGCTTTTC TAGCTGTTAT TCCTAAACAA
    ATGGAGAGAG AAACCTGTAA TGACCCCAAG CCTGTTGAGT TTCAAGGGCA CCAAGTGAAA
    GGCTCCGCAT CTAGCGCTGT GATGGTCAGA GGACACAGCT CACAGGTTGA ATGCAGTCAG
    CTTTCGGATA GCACTGTGTA TGGAAGCTGC ACGACAAACA CTTGTTTTTT GACACCAGAG
    TTGCCTAGCA CTCGTATGCA GATGGGCTTC TTGCAGTTCC CAAACGAAGA AAACTTTACA
    AAAGGAGATA CTAGCTTTAC TATTGAAGAC AATTTACAAG TGAGAGCTGA GTTTAATAAG
    GACTTGACTA AAAATGATTC AATTTTAGAT TTCCCTCCTA AAGATTCAGA CCATTTATAT
    AATTATTTGG ATTATAATTT TAAATCAACA GAGAGGACTT CGTGGGAAAA AAATAAGGTG
    ACGTCACCAG AACAGAAGAT CTCAACATTA AGCCCTGTTT CTACTTTATC TTTTAACTCG
    AGAGATGATG ACTTCATGCT AGAATTCTCT GAGGAGTCCC TGAAAGGGCA AACTTTACCT
    GTAATGAAAA AATCAAGTTC TCTGGAGAAT AAGAACCTTC AGAGGCTTTC CTTTGAAGTA
    AGTAGAACCC GAGCTCCACT TGTTGCTTTG CCGCCTGCCA GTGGGCCACC TGACTCAGAT
    TCTTGTTCAC GTATGACGGA CCGTGACAGG CAGAAGGCTT CTGGTCCTCC CATATTCAGC
    CTGCGTGAAG AGTCAGCAGT TCCTTCTTTC AGCTTTGGGC CCAAGGACCA AAGCGAAGCT
    TCTTTCTCTA ACGAATCTAG GGAAGTGACT CCAAGTGAGG TTTCCCTTCT TGATAATACA
    TCTACTCAAA GCAAGTGGCT GAAATATCAA AATACACGCC AGTGCAACTT GACTACTCCA
    GACAGAGTTG ATGAGAAAGT AACAGACGGC TTCTTTGCTA AGGCTGTTTC TGGGATGCGT
    TTTAGTAACA CAGGTGAAAG AGAGAATGAT GCTGTGAATG AAAGTTCTTT AGATTCTGTG
    TATTTACAAA TGATGAAACA CATGCTTCAA CAGCAGCAGC AGGATTTTAG CAGTCAAGAT
    TCGGTTTGCA GAAAGAAGGC ATTTTCTTTG AATATAAACG AGGCTTCTAA GACTGAGGAA
    GTTCAGAATA TGTTAGGAGA GTATGCCTGC TACAACTGCA GTGTAACAGG AGATTTACAG
    GAGGTAAAAG ACTCTGAGCT ATGCTTCCCA AGTGGGCAGA AAGTAAAATC TGCATGTCTT
    CCCCAAAGGC AGATTCACAT ACCAGCTGTT TTTCAGTCTC CTGCGCATTA TAAGCAGGTT
    TTTACATCTT GTCTCACAGA ACATTTAAAT ATATTGCTAT TTGGATTGGC ACAAAGGTTG
    CACAAAGCTC TTTCAAAAGT TGACATATCA TTTTACACAT CATCAAATGG AGACAAACTG
    AAAAATGTAG AAAATAATGT GCCATCCTGC CATCATCAGC AGCCTGCAAA ACTGGTCATG
    GTTAAAAAGG AAGGTCCAAA TAAGGGTCGT CTCTTTTATA CCTGTGATGG ACCCAAAGCT
    GACCAATGTA AATTTTTTAA GTGGTTTGAA GAAATGACCC CAGGATATTT AACACAGGAA
    AAATCTCTAC CTAGCATGGT TTTAAGTGAT ATAAAAAGTA TTGGCTTCTA CTTAAGAAGT
    CAAAAGATAC CCCTCTATGA GGAATGCCAA CTTTTCGTGA GGAAAGGATT TGATTTTCAA
    AGAAAACGGT ATGGTAAACT AAAGAAGTTT ACTACTGTAG ATCCTGAATT TTATAATGAA
    CCTAAGAGCA AACTTTATCT TAAACTGAGT CGGAAAGAAA GTTCATCTGC TTACAGCAAA
    GATGATCTTT GGGTGGTTTC GAAAACCCTA GACTTTGAAT TGGATACTTT TATTGCATGC
    AGTACTTTCT TTGGACCATC ATCTATCAAT GAGGTAGAGC TTCTACCTTT GAAAGGTTAT
    TTCCCTTCGA ACTGGCCCAC TAACACAGTG GTCCACGCCT TATTGGTTTG TAATGCCAGC
    ACAGAGCTGA CCACTTTAAA AAATATTCGG GACTGCTTTA ATCCAGCTAC TCTACCTCTA
    ACACAGTACC TCTTAACAAT GTCTTCATCA ACTACAACGA GCAACAAGAG AGTCAATAAG
    AGAAAGTTTA TCCCACCAGC CTTCTCAAAT ATCAACACAA AATTTGAACT ACTCAGTATA
    GAAGCAACAT TGAAGTTGGC TAGTGAGTTA ATTCAGGTGC ACAAGTTAAA CAAGGATCAA
    GCCGCAGCTC TAATTCAGAT AGCTCAAATG ATGGCATCAC GCCAGAGTGT CGAAGAAGCG
    AAGGAACTGG CAATTCATAC CCTCCCTATC ACGATCATAC ACGGCGTTTT CGGAGCAGGA
    AAGAGTTACT TGCTAGCTGT GGTGATTTTG TTTTTTGTGC AACTATTTGA AAAATGTGAA
    GCTCTTACAG TTGGAAATGC AAGACCGTGG AAACTTCTGA TTTCTTCTTC TACTAATGTA
    GCTGTTGACA GAGTGCTTCT TGGGCTTCTT AGTCTTGGAT TTGAAAAGTT TGTCAGAGTT
    GGGAGTGTCA GAAAGATTGC CAAGCCAGTT TTACCTTACA GTTTGTATGC TGGCTCAGAA
    AATGAAAATG AGCAATTAAA GGAACTGCAT GCACTCATGA AGGAAGACTT GACTCCTGTG
    GAAAGAGTCT ACGTGAGGAA AAGTATTGAG CAGCATAAAC TGGGGACCAA TAAAACCCTG
    CTGAAACAGG TTCGAGTAGT TGGAGTTACC TGTGCAGCCT GCCCATTCCC ATGCATGAAT
    GATCTCAAAT TCCCTGTGGT TGTCCTGGAT GAGTGTAGTC AAATGACAGA ACCGGCTTCT
    CTCCTTCCCA TTGCAAGGTT TGAGTGTGAA AAGCTGATAC TTGTTGGAGA TCCCAAACAG
    CTTCCTCCTA CTATTCAGGG TTCTGATGCA GCTCATGAAA ATGGACTGGA ACAAACTCTT
    TTTGACCGAC TCTGCTTAAT GGGCCACAAG CCAATTCTGT TGAGAACTCA GTACCGTTGT
    CACCCCACAA TCAGCGCTAT TGCTAATGAT CTGTTTTATG AAGGAAATCT CATGAATGGC
    ATTTCCGAAA CAGAGAGGAG CCCTTTACTG GAATGGCTCC CAACTCTGTG TTTCTATAAC
    GTAAAAGGAC TGGAACAGAT TGAAAGAGGT AACAGCTTTC ATAATGTGGC AGAAGCTGCT
    TTTACACTCA AGTTGATTCA ATCACTGATT GCGAGTGGAA TAGCGGGCTC CATGATTGGG
    GTGATAACAT TATACAAATC CCAGATGTAC AAGCTCTGTC ATCTACTCAG TGCGATGGAC
    TTCGACCATC CCAACTTGAA AGCTGTGCAG GTGTCCACAG TAGATGCTTT TCAGGGAGCT
    GAAAAGGAGA TCATTATTCT GTCCTGTGTA AGGACCAGAC AAGTAGGATT TATTGATTCA
    GAAAAAAGAA TGAATGTTGC ATTGACCAGA GGAAGGAGGC ATTTGTTGAT TGTAGGAAAT
    TTAGCCTGTC TGAGGAAAAA TCGACTATGG GGGCGTGTGA TCCAACACTG TGAAGGGAGG
    GAAGGTGGAT TGCAACATGC AAGCCAGTAT GAACCACAAC TGGACCATCT CCTTAAAGAT
    TATCTTGAAA AACAAGCAGA AGAAAAACAG AAGAAAAAGG CTGAAAAAGA TAAATTGACA
    GATAAAAAAT CTTATTCACA AAAAGACATG GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.