NPC1L1 cDNA ORF clone, Bos taurus(cattle)

The following NPC1L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPC1L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa03944 XM_588051.7
Latest version!
Bos taurus NPC1-like 1 (NPC1L1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa03944 XM_002686890.5
Latest version!
Bos taurus NPC1 like intracellular cholesterol transporter 1 (NPC1L1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa03944 XM_002686890.4 Bos taurus NPC1-like 1 (NPC1L1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa03944
    Clone ID Related Accession (Same CDS sequence) XM_002686890.4 , XM_588051.7 , XM_002686890.5
    Accession Version XM_002686890.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product Niemann-Pick C1-like protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103902.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002686890.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGAGA CTGGCCTGAA GGGCTGGTTG CTCTGGGCCC TGCTCCTGCA CTCGGCCCAG 
    AGTGAGGTCT ACACACCCAT ACACCAGCCT GGCTACTGTG CCTTCTACGA TGAGTGCGGG
    AAGAACCCGG AGCTGTCCGG AAGCCTGGCT TCGCTGTCCA ATGTGTCCTG CCTGGATAAC
    TCACCTGCTC GCCACATCAC AGGTGACCAC CTGGCCCTCC TACAAAGCAT CTGTCCCCGT
    CTCTACACTG GCGCCAGCAC CACCTACGCC TGCTGCTCCT CCAAGCAGCT GGTGGCCCTG
    GACATGAGCC TGCGGATCAC CAAGGCCCTC CTCACTCGCT GTCCCGCCTG CTCTGACAAC
    TTCGTGAGCC TGCACTGCCA CAACACCTGC AGCCCCAACC AAAGCCTCTT CATCAATGTG
    ACCCGAGTGG TCACACAGGG GGACAGCCAG TCCCAGGCCG TGGTGGCCTA CGAGGCCTTC
    TACCAGCGCA GCTTTGCCGA GCAGACCTAC AACTCCTGCA GCCGTGTGCG CATCCCCGCA
    GCTGCCACAC TGGCCGTGGG CTCCATGTGT GGCGTCTATG GCTCTGCCCT CTGCAACGCC
    CAGCGCTGGC TCAATTTCCA GGGGGACACG AGCAATGGCC TGGCACCCCT GGACATCACC
    TTCCACCTGT GGGAGCCTAG CCAGGCCGAG GGGAGCACGA TACAGCCTCT AAATGATGAG
    GTCGTGCCCT GCAACCAGTC CCAGGGGGAC GGTGCAGCGG CCTGTTCCTG CCAGGACTGT
    GCCGCCTCCT GCCCCGTGAT TGCCCAGCCC CGGGCCCTGG ACCCCACCTT CCGCCTGGGC
    CGGATGGAAG GGAGCCTGGT CCTCATTATC ATCCTCTGTT CTCTCTTTGT CTTGCTCACG
    GCCTTCCTCC TGCGGTCCCG CCTGGCCGAG TGGTGCAGGG GCAAGCGCAA GACGCCCAAG
    CCCAAGGCAA GCATCAACCT GGCCCACAGG CTTAGCCTCT CCACCCACAC CCTCCTCAGC
    CGCTGCTTCC AGTGCTGGGG CACTTGGGTG GCCTCATGGC CACTGACCAT CCTGGCGGTA
    TCTGTGATTG TGGTGGTGGC CCTGGCAGGG GGCCTGGCTT TTATAGAACT GACCACGGAC
    CCCGTGGAGT TGTGGTCAGC CCCCAACAGC CTAGCCCGAC GTGAGAAGGC GTTCCACGAC
    AAGTATTTCG GCCCCTTCTT CCGAACCAGC CAGGTGTTCA TGACAGCACC TCACCGGCCC
    AGCTATAGGT ATGACTCCCT GCTGCTGGGG CCCAAGAACT TCAGCGGGAT CTTGTCCTCG
    GATCTGCTGC TGGAGGTGCT GGAATTGCAG GAGAGGCTGC GGCATCTGCA GGTGTGGTCG
    CCAGAGGAGC AGCGGAACGT CTCCCTGCGG GACACCTGCT ATGCCCCCCT CAACCCACAT
    AACGCCAGTC TGTCCGACTG CTGCGTCAAC AGCCTCCTGC AGTACTTCCA GAACAACCGC
    ACTCAGCTGC TGCTCACAGC CAACCAGACG CTGTCAGGGC AGACCGCCCA GGTGGACTGG
    AGGGACCACT TCCTCTACTG CGCCAATGCC CCCCTCACCT ACAAAGATGG CACGGCCCTG
    GCCCTGAGCT GCATGGCTGA CTATGGGGCA CCCATCTTCC CCTTCCTCGC CGTGGGGGGC
    TACAAAGGAA AGGACTATTC TGAGGCAGAG GCCCTGATCA TGACCTTCTC CCTCAACAAT
    TACCCTCCTG GGGACCCCAA GCTGGCCCAG GCTAAGCTCT GGGAGGCAGC CTTCTTGGAG
    GAGATGCGAG CCTTTCAGCG TCGGACGGCT GGTGTGTTCC AGGTCACATT CATGGCGGAG
    CGTTCCCTGG AGGATGAGAT CAACAGCACC ACGGCCGAGG ACCTGCCCGT CTTTGCTGTT
    AGCTACCTTG TCATCTTCCT GTACATCTCC CTGGCCCTGG GCAGCTACTC CAGCTGGCGT
    CGGGTACCGG TGGACTCCAA GGCCACGCTG GGCCTGGGCG GGGTGGCCGT GGTGCTGGGA
    GCAGTCGTGG CCTCTATGGG CTTCTTCTCC TACCTGGGTG TCCCTTCCTC ACTGGTGATC
    CTTCAAGTGG TGCCCTTCCT GGTGCTTGCC GTAGGGGCCG ACAACATCTT CATCTTCGTT
    CTTGAGTACC AGAGGCTGCC TCGGAGGCCT GGGGAAGAAC GGGAGGCCCA CATCGGCCGA
    GCCCTGGGCA GAGTGGCCCC CAGCATGCTT CTCTGCAGCC TCTCTGAGGC CATCTGCTTC
    TTCTTAGGAG CCCTGACCCC CATGCCAGCT GTACGGACCT TTGCCCTGAC CTCCGGCTTT
    GCAGTGCTTC TTGACTTCCT GCTACAGATG TCAGCCTTTG TGGCCCTGCT CTCCCTCGAC
    AGCAGGAGGC AAGAGGCCTC CCGGATGGAC ATCTGCTGTT GCAAGACAGC CCAGAAGCTG
    CCCCCGCCGA GCCAGGATGA GGGGCTCCTG CTCCGATTCT TCCGCAAGTT CTACGTCCCA
    TTCCTGTTGC ACTGGCTCAC CCGCGTGGTC GTGGTGCTGC TCTTCCTGGC CCTGTTTGCA
    GCGAGCCTCT ATTTCATGTG CTACATTAAT GTGGGGCTGG ACCAGCAGCT GGCTCTGCCC
    AAGGACTCAT ACCTGATAGA CTATTTCCTC TTTATGAACC GCTACTTTGA GGTGGGGGCC
    CCGGTCTATT TTGTTACCAC TGGGGGCTAC AACTTCTCCA GTGAGGAAGG AATGAATGCC
    ATCTGCTCCA GTGCAGGCTG CAACAACTTC TCCTTAACCC AGAAGATCCA GTACGCCACC
    GACTTCCCTG ATATGTCTTA CCTGGCCATC CCTGCCTCCT CCTGGGTGGA CGACTTCATT
    GACTGGCTGA CTTCATCTTC CTGCTGCCGC CTTTATATCT CTGGTCCCAA TAAAGATGAG
    TTCTGCCCCT CGACTGTCAA CTCCCTGGCC TGCTTGAAGA CCTGTGTGAG CCCCACAGCG
    GGCTCTGCCC GGCCATCAGT GGAGCAGTTC CACAAATATC TTCCCTGGTT TCTGAGTGAC
    GAGCCCAACA TCAAATGTCC CAAAGGTGGC CTGGCAGCAT ACAGCACCTC AGTGAATATG
    AGCTCAGATG GCCAGATTTT AGCCTCGCGG TTCATGGCCT ACAACAAGCC GCTGAAGAAC
    TCGCGGGATT TCACGGAGGC TCTGCGGGCA ACTCGGGCAC TGGCAGCCAA CATCACCGCT
    GACCTGCGGA AGGTGCCAGG CACTGACCCA GATTTTGAGG TCTTCCCTTA CTCGGTCACC
    AACGTGTTCT ATGAGCAGTA CCTGACCATA GTCCCCGAGG GGCTCTTTAT GCTCACCATC
    TGCCTGGTGC CCACCTTTGT CGTCTGCTGC TTCCTGCTGG GCATGGACGT CCGCTCCGGC
    CTCCTCAACC TCTTCTCTAT CATCATGATC CTGGTGGACA CTGTGGGCTT CATGATGCTC
    TGGGACATCA GCTACAATGC TGTGTCCCTC ATCAACCTGG TCACGGCGGT GGGCATCTCT
    GTGGAGTTTG TGTCCCATAT CACCCGCTCC TTTGCCATCA GCACCAAGCC CACCCGGCTG
    GAGAGGGCTA AGGAGGCCAC CATCTCCATG GGCAGTGCGG TGTTTGCAGG AGTAGCCATG
    ACCAACCTGC CTGGCATCCT TGTCCTGGGC CTGGCCAAGG CGCAGCTCAT CCAGATCTTT
    TTCTTCCGTC TCAACCTCCT CATCACTGTG CTGGGCCTGC TGCATGGCCT GGTCTTCCTG
    CCCGTCATCC TCAGCTACCT GGGGCCTGAT GTCAACCCAG CTCTGGTGCA GCAGCAGAAG
    CAGCAGGAGG AGGCAGCTGC GGCCAAGGAG ACCTCTTGCT CAAAGCACCC CGCTCAGATG
    AGCACTGATT ATGGTGTCTA CGTCAACTGC AGCTTTGAAC ATCCTGCCAA GAGTGTAGGA
    GGCCTCGAGA GCTCTCCATC AGAGAACAGG CAGAAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002686936.1
    CDS130..4131
    Translation

    Target ORF information:

    RefSeq Version XM_002686890.4
    Organism Bos taurus(cattle)
    Definition Bos taurus NPC1-like 1 (NPC1L1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002686890.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGAGA CTGGCCTGAA GGGCTGGTTG CTCTGGGCCC TGCTCCTGCA CTCGGCCCAG 
    AGTGAGGTCT ACACACCCAT ACACCAGCCT GGCTACTGTG CCTTCTACGA TGAGTGCGGG
    AAGAACCCGG AGCTGTCCGG AAGCCTGGCT TCGCTGTCCA ATGTGTCCTG CCTGGATAAC
    TCACCTGCTC GCCACATCAC AGGTGACCAC CTGGCCCTCC TACAAAGCAT CTGTCCCCGT
    CTCTACACTG GCGCCAGCAC CACCTACGCC TGCTGCTCCT CCAAGCAGCT GGTGGCCCTG
    GACATGAGCC TGCGGATCAC CAAGGCCCTC CTCACTCGCT GTCCCGCCTG CTCTGACAAC
    TTCGTGAGCC TGCACTGCCA CAACACCTGC AGCCCCAACC AAAGCCTCTT CATCAATGTG
    ACCCGAGTGG TCACACAGGG GGACAGCCAG TCCCAGGCCG TGGTGGCCTA CGAGGCCTTC
    TACCAGCGCA GCTTTGCCGA GCAGACCTAC AACTCCTGCA GCCGTGTGCG CATCCCCGCA
    GCTGCCACAC TGGCCGTGGG CTCCATGTGT GGCGTCTATG GCTCTGCCCT CTGCAACGCC
    CAGCGCTGGC TCAATTTCCA GGGGGACACG AGCAATGGCC TGGCACCCCT GGACATCACC
    TTCCACCTGT GGGAGCCTAG CCAGGCCGAG GGGAGCACGA TACAGCCTCT AAATGATGAG
    GTCGTGCCCT GCAACCAGTC CCAGGGGGAC GGTGCAGCGG CCTGTTCCTG CCAGGACTGT
    GCCGCCTCCT GCCCCGTGAT TGCCCAGCCC CGGGCCCTGG ACCCCACCTT CCGCCTGGGC
    CGGATGGAAG GGAGCCTGGT CCTCATTATC ATCCTCTGTT CTCTCTTTGT CTTGCTCACG
    GCCTTCCTCC TGCGGTCCCG CCTGGCCGAG TGGTGCAGGG GCAAGCGCAA GACGCCCAAG
    CCCAAGGCAA GCATCAACCT GGCCCACAGG CTTAGCCTCT CCACCCACAC CCTCCTCAGC
    CGCTGCTTCC AGTGCTGGGG CACTTGGGTG GCCTCATGGC CACTGACCAT CCTGGCGGTA
    TCTGTGATTG TGGTGGTGGC CCTGGCAGGG GGCCTGGCTT TTATAGAACT GACCACGGAC
    CCCGTGGAGT TGTGGTCAGC CCCCAACAGC CTAGCCCGAC GTGAGAAGGC GTTCCACGAC
    AAGTATTTCG GCCCCTTCTT CCGAACCAGC CAGGTGTTCA TGACAGCACC TCACCGGCCC
    AGCTATAGGT ATGACTCCCT GCTGCTGGGG CCCAAGAACT TCAGCGGGAT CTTGTCCTCG
    GATCTGCTGC TGGAGGTGCT GGAATTGCAG GAGAGGCTGC GGCATCTGCA GGTGTGGTCG
    CCAGAGGAGC AGCGGAACGT CTCCCTGCGG GACACCTGCT ATGCCCCCCT CAACCCACAT
    AACGCCAGTC TGTCCGACTG CTGCGTCAAC AGCCTCCTGC AGTACTTCCA GAACAACCGC
    ACTCAGCTGC TGCTCACAGC CAACCAGACG CTGTCAGGGC AGACCGCCCA GGTGGACTGG
    AGGGACCACT TCCTCTACTG CGCCAATGCC CCCCTCACCT ACAAAGATGG CACGGCCCTG
    GCCCTGAGCT GCATGGCTGA CTATGGGGCA CCCATCTTCC CCTTCCTCGC CGTGGGGGGC
    TACAAAGGAA AGGACTATTC TGAGGCAGAG GCCCTGATCA TGACCTTCTC CCTCAACAAT
    TACCCTCCTG GGGACCCCAA GCTGGCCCAG GCTAAGCTCT GGGAGGCAGC CTTCTTGGAG
    GAGATGCGAG CCTTTCAGCG TCGGACGGCT GGTGTGTTCC AGGTCACATT CATGGCGGAG
    CGTTCCCTGG AGGATGAGAT CAACAGCACC ACGGCCGAGG ACCTGCCCGT CTTTGCTGTT
    AGCTACCTTG TCATCTTCCT GTACATCTCC CTGGCCCTGG GCAGCTACTC CAGCTGGCGT
    CGGGTACCGG TGGACTCCAA GGCCACGCTG GGCCTGGGCG GGGTGGCCGT GGTGCTGGGA
    GCAGTCGTGG CCTCTATGGG CTTCTTCTCC TACCTGGGTG TCCCTTCCTC ACTGGTGATC
    CTTCAAGTGG TGCCCTTCCT GGTGCTTGCC GTAGGGGCCG ACAACATCTT CATCTTCGTT
    CTTGAGTACC AGAGGCTGCC TCGGAGGCCT GGGGAAGAAC GGGAGGCCCA CATCGGCCGA
    GCCCTGGGCA GAGTGGCCCC CAGCATGCTT CTCTGCAGCC TCTCTGAGGC CATCTGCTTC
    TTCTTAGGAG CCCTGACCCC CATGCCAGCT GTACGGACCT TTGCCCTGAC CTCCGGCTTT
    GCAGTGCTTC TTGACTTCCT GCTACAGATG TCAGCCTTTG TGGCCCTGCT CTCCCTCGAC
    AGCAGGAGGC AAGAGGCCTC CCGGATGGAC ATCTGCTGTT GCAAGACAGC CCAGAAGCTG
    CCCCCGCCGA GCCAGGATGA GGGGCTCCTG CTCCGATTCT TCCGCAAGTT CTACGTCCCA
    TTCCTGTTGC ACTGGCTCAC CCGCGTGGTC GTGGTGCTGC TCTTCCTGGC CCTGTTTGCA
    GCGAGCCTCT ATTTCATGTG CTACATTAAT GTGGGGCTGG ACCAGCAGCT GGCTCTGCCC
    AAGGACTCAT ACCTGATAGA CTATTTCCTC TTTATGAACC GCTACTTTGA GGTGGGGGCC
    CCGGTCTATT TTGTTACCAC TGGGGGCTAC AACTTCTCCA GTGAGGAAGG AATGAATGCC
    ATCTGCTCCA GTGCAGGCTG CAACAACTTC TCCTTAACCC AGAAGATCCA GTACGCCACC
    GACTTCCCTG ATATGTCTTA CCTGGCCATC CCTGCCTCCT CCTGGGTGGA CGACTTCATT
    GACTGGCTGA CTTCATCTTC CTGCTGCCGC CTTTATATCT CTGGTCCCAA TAAAGATGAG
    TTCTGCCCCT CGACTGTCAA CTCCCTGGCC TGCTTGAAGA CCTGTGTGAG CCCCACAGCG
    GGCTCTGCCC GGCCATCAGT GGAGCAGTTC CACAAATATC TTCCCTGGTT TCTGAGTGAC
    GAGCCCAACA TCAAATGTCC CAAAGGTGGC CTGGCAGCAT ACAGCACCTC AGTGAATATG
    AGCTCAGATG GCCAGATTTT AGCCTCGCGG TTCATGGCCT ACAACAAGCC GCTGAAGAAC
    TCGCGGGATT TCACGGAGGC TCTGCGGGCA ACTCGGGCAC TGGCAGCCAA CATCACCGCT
    GACCTGCGGA AGGTGCCAGG CACTGACCCA GATTTTGAGG TCTTCCCTTA CTCGGTCACC
    AACGTGTTCT ATGAGCAGTA CCTGACCATA GTCCCCGAGG GGCTCTTTAT GCTCACCATC
    TGCCTGGTGC CCACCTTTGT CGTCTGCTGC TTCCTGCTGG GCATGGACGT CCGCTCCGGC
    CTCCTCAACC TCTTCTCTAT CATCATGATC CTGGTGGACA CTGTGGGCTT CATGATGCTC
    TGGGACATCA GCTACAATGC TGTGTCCCTC ATCAACCTGG TCACGGCGGT GGGCATCTCT
    GTGGAGTTTG TGTCCCATAT CACCCGCTCC TTTGCCATCA GCACCAAGCC CACCCGGCTG
    GAGAGGGCTA AGGAGGCCAC CATCTCCATG GGCAGTGCGG TGTTTGCAGG AGTAGCCATG
    ACCAACCTGC CTGGCATCCT TGTCCTGGGC CTGGCCAAGG CGCAGCTCAT CCAGATCTTT
    TTCTTCCGTC TCAACCTCCT CATCACTGTG CTGGGCCTGC TGCATGGCCT GGTCTTCCTG
    CCCGTCATCC TCAGCTACCT GGGGCCTGAT GTCAACCCAG CTCTGGTGCA GCAGCAGAAG
    CAGCAGGAGG AGGCAGCTGC GGCCAAGGAG ACCTCTTGCT CAAAGCACCC CGCTCAGATG
    AGCACTGATT ATGGTGTCTA CGTCAACTGC AGCTTTGAAC ATCCTGCCAA GAGTGTAGGA
    GGCCTCGAGA GCTCTCCATC AGAGAACAGG CAGAAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03944
    Clone ID Related Accession (Same CDS sequence) XM_002686890.4 , XM_588051.7 , XM_002686890.5
    Accession Version XM_588051.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product Niemann-Pick C1-like protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644730.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_588051.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGAGA CTGGCCTGAA GGGCTGGTTG CTCTGGGCCC TGCTCCTGCA CTCGGCCCAG 
    AGTGAGGTCT ACACACCCAT ACACCAGCCT GGCTACTGTG CCTTCTACGA TGAGTGCGGG
    AAGAACCCGG AGCTGTCCGG AAGCCTGGCT TCGCTGTCCA ATGTGTCCTG CCTGGATAAC
    TCACCTGCTC GCCACATCAC AGGTGACCAC CTGGCCCTCC TACAAAGCAT CTGTCCCCGT
    CTCTACACTG GCGCCAGCAC CACCTACGCC TGCTGCTCCT CCAAGCAGCT GGTGGCCCTG
    GACATGAGCC TGCGGATCAC CAAGGCCCTC CTCACTCGCT GTCCCGCCTG CTCTGACAAC
    TTCGTGAGCC TGCACTGCCA CAACACCTGC AGCCCCAACC AAAGCCTCTT CATCAATGTG
    ACCCGAGTGG TCACACAGGG GGACAGCCAG TCCCAGGCCG TGGTGGCCTA CGAGGCCTTC
    TACCAGCGCA GCTTTGCCGA GCAGACCTAC AACTCCTGCA GCCGTGTGCG CATCCCCGCA
    GCTGCCACAC TGGCCGTGGG CTCCATGTGT GGCGTCTATG GCTCTGCCCT CTGCAACGCC
    CAGCGCTGGC TCAATTTCCA GGGGGACACG AGCAATGGCC TGGCACCCCT GGACATCACC
    TTCCACCTGT GGGAGCCTAG CCAGGCCGAG GGGAGCACGA TACAGCCTCT AAATGATGAG
    GTCGTGCCCT GCAACCAGTC CCAGGGGGAC GGTGCAGCGG CCTGTTCCTG CCAGGACTGT
    GCCGCCTCCT GCCCCGTGAT TGCCCAGCCC CGGGCCCTGG ACCCCACCTT CCGCCTGGGC
    CGGATGGAAG GGAGCCTGGT CCTCATTATC ATCCTCTGTT CTCTCTTTGT CTTGCTCACG
    GCCTTCCTCC TGCGGTCCCG CCTGGCCGAG TGGTGCAGGG GCAAGCGCAA GACGCCCAAG
    CCCAAGGCAA GCATCAACCT GGCCCACAGG CTTAGCCTCT CCACCCACAC CCTCCTCAGC
    CGCTGCTTCC AGTGCTGGGG CACTTGGGTG GCCTCATGGC CACTGACCAT CCTGGCGGTA
    TCTGTGATTG TGGTGGTGGC CCTGGCAGGG GGCCTGGCTT TTATAGAACT GACCACGGAC
    CCCGTGGAGT TGTGGTCAGC CCCCAACAGC CTAGCCCGAC GTGAGAAGGC GTTCCACGAC
    AAGTATTTCG GCCCCTTCTT CCGAACCAGC CAGGTGTTCA TGACAGCACC TCACCGGCCC
    AGCTATAGGT ATGACTCCCT GCTGCTGGGG CCCAAGAACT TCAGCGGGAT CTTGTCCTCG
    GATCTGCTGC TGGAGGTGCT GGAATTGCAG GAGAGGCTGC GGCATCTGCA GGTGTGGTCG
    CCAGAGGAGC AGCGGAACGT CTCCCTGCGG GACACCTGCT ATGCCCCCCT CAACCCACAT
    AACGCCAGTC TGTCCGACTG CTGCGTCAAC AGCCTCCTGC AGTACTTCCA GAACAACCGC
    ACTCAGCTGC TGCTCACAGC CAACCAGACG CTGTCAGGGC AGACCGCCCA GGTGGACTGG
    AGGGACCACT TCCTCTACTG CGCCAATGCC CCCCTCACCT ACAAAGATGG CACGGCCCTG
    GCCCTGAGCT GCATGGCTGA CTATGGGGCA CCCATCTTCC CCTTCCTCGC CGTGGGGGGC
    TACAAAGGAA AGGACTATTC TGAGGCAGAG GCCCTGATCA TGACCTTCTC CCTCAACAAT
    TACCCTCCTG GGGACCCCAA GCTGGCCCAG GCTAAGCTCT GGGAGGCAGC CTTCTTGGAG
    GAGATGCGAG CCTTTCAGCG TCGGACGGCT GGTGTGTTCC AGGTCACATT CATGGCGGAG
    CGTTCCCTGG AGGATGAGAT CAACAGCACC ACGGCCGAGG ACCTGCCCGT CTTTGCTGTT
    AGCTACCTTG TCATCTTCCT GTACATCTCC CTGGCCCTGG GCAGCTACTC CAGCTGGCGT
    CGGGTACCGG TGGACTCCAA GGCCACGCTG GGCCTGGGCG GGGTGGCCGT GGTGCTGGGA
    GCAGTCGTGG CCTCTATGGG CTTCTTCTCC TACCTGGGTG TCCCTTCCTC ACTGGTGATC
    CTTCAAGTGG TGCCCTTCCT GGTGCTTGCC GTAGGGGCCG ACAACATCTT CATCTTCGTT
    CTTGAGTACC AGAGGCTGCC TCGGAGGCCT GGGGAAGAAC GGGAGGCCCA CATCGGCCGA
    GCCCTGGGCA GAGTGGCCCC CAGCATGCTT CTCTGCAGCC TCTCTGAGGC CATCTGCTTC
    TTCTTAGGAG CCCTGACCCC CATGCCAGCT GTACGGACCT TTGCCCTGAC CTCCGGCTTT
    GCAGTGCTTC TTGACTTCCT GCTACAGATG TCAGCCTTTG TGGCCCTGCT CTCCCTCGAC
    AGCAGGAGGC AAGAGGCCTC CCGGATGGAC ATCTGCTGTT GCAAGACAGC CCAGAAGCTG
    CCCCCGCCGA GCCAGGATGA GGGGCTCCTG CTCCGATTCT TCCGCAAGTT CTACGTCCCA
    TTCCTGTTGC ACTGGCTCAC CCGCGTGGTC GTGGTGCTGC TCTTCCTGGC CCTGTTTGCA
    GCGAGCCTCT ATTTCATGTG CTACATTAAT GTGGGGCTGG ACCAGCAGCT GGCTCTGCCC
    AAGGACTCAT ACCTGATAGA CTATTTCCTC TTTATGAACC GCTACTTTGA GGTGGGGGCC
    CCGGTCTATT TTGTTACCAC TGGGGGCTAC AACTTCTCCA GTGAGGAAGG AATGAATGCC
    ATCTGCTCCA GTGCAGGCTG CAACAACTTC TCCTTAACCC AGAAGATCCA GTACGCCACC
    GACTTCCCTG ATATGTCTTA CCTGGCCATC CCTGCCTCCT CCTGGGTGGA CGACTTCATT
    GACTGGCTGA CTTCATCTTC CTGCTGCCGC CTTTATATCT CTGGTCCCAA TAAAGATGAG
    TTCTGCCCCT CGACTGTCAA CTCCCTGGCC TGCTTGAAGA CCTGTGTGAG CCCCACAGCG
    GGCTCTGCCC GGCCATCAGT GGAGCAGTTC CACAAATATC TTCCCTGGTT TCTGAGTGAC
    GAGCCCAACA TCAAATGTCC CAAAGGTGGC CTGGCAGCAT ACAGCACCTC AGTGAATATG
    AGCTCAGATG GCCAGATTTT AGCCTCGCGG TTCATGGCCT ACAACAAGCC GCTGAAGAAC
    TCGCGGGATT TCACGGAGGC TCTGCGGGCA ACTCGGGCAC TGGCAGCCAA CATCACCGCT
    GACCTGCGGA AGGTGCCAGG CACTGACCCA GATTTTGAGG TCTTCCCTTA CTCGGTCACC
    AACGTGTTCT ATGAGCAGTA CCTGACCATA GTCCCCGAGG GGCTCTTTAT GCTCACCATC
    TGCCTGGTGC CCACCTTTGT CGTCTGCTGC TTCCTGCTGG GCATGGACGT CCGCTCCGGC
    CTCCTCAACC TCTTCTCTAT CATCATGATC CTGGTGGACA CTGTGGGCTT CATGATGCTC
    TGGGACATCA GCTACAATGC TGTGTCCCTC ATCAACCTGG TCACGGCGGT GGGCATCTCT
    GTGGAGTTTG TGTCCCATAT CACCCGCTCC TTTGCCATCA GCACCAAGCC CACCCGGCTG
    GAGAGGGCTA AGGAGGCCAC CATCTCCATG GGCAGTGCGG TGTTTGCAGG AGTAGCCATG
    ACCAACCTGC CTGGCATCCT TGTCCTGGGC CTGGCCAAGG CGCAGCTCAT CCAGATCTTT
    TTCTTCCGTC TCAACCTCCT CATCACTGTG CTGGGCCTGC TGCATGGCCT GGTCTTCCTG
    CCCGTCATCC TCAGCTACCT GGGGCCTGAT GTCAACCCAG CTCTGGTGCA GCAGCAGAAG
    CAGCAGGAGG AGGCAGCTGC GGCCAAGGAG ACCTCTTGCT CAAAGCACCC CGCTCAGATG
    AGCACTGATT ATGGTGTCTA CGTCAACTGC AGCTTTGAAC ATCCTGCCAA GAGTGTAGGA
    GGCCTCGAGA GCTCTCCATC AGAGAACAGG CAGAAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_588051.4
    CDS130..4131
    Translation

    Target ORF information:

    RefSeq Version XM_588051.7
    Organism Bos taurus(cattle)
    Definition Bos taurus NPC1-like 1 (NPC1L1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_588051.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGAGA CTGGCCTGAA GGGCTGGTTG CTCTGGGCCC TGCTCCTGCA CTCGGCCCAG 
    AGTGAGGTCT ACACACCCAT ACACCAGCCT GGCTACTGTG CCTTCTACGA TGAGTGCGGG
    AAGAACCCGG AGCTGTCCGG AAGCCTGGCT TCGCTGTCCA ATGTGTCCTG CCTGGATAAC
    TCACCTGCTC GCCACATCAC AGGTGACCAC CTGGCCCTCC TACAAAGCAT CTGTCCCCGT
    CTCTACACTG GCGCCAGCAC CACCTACGCC TGCTGCTCCT CCAAGCAGCT GGTGGCCCTG
    GACATGAGCC TGCGGATCAC CAAGGCCCTC CTCACTCGCT GTCCCGCCTG CTCTGACAAC
    TTCGTGAGCC TGCACTGCCA CAACACCTGC AGCCCCAACC AAAGCCTCTT CATCAATGTG
    ACCCGAGTGG TCACACAGGG GGACAGCCAG TCCCAGGCCG TGGTGGCCTA CGAGGCCTTC
    TACCAGCGCA GCTTTGCCGA GCAGACCTAC AACTCCTGCA GCCGTGTGCG CATCCCCGCA
    GCTGCCACAC TGGCCGTGGG CTCCATGTGT GGCGTCTATG GCTCTGCCCT CTGCAACGCC
    CAGCGCTGGC TCAATTTCCA GGGGGACACG AGCAATGGCC TGGCACCCCT GGACATCACC
    TTCCACCTGT GGGAGCCTAG CCAGGCCGAG GGGAGCACGA TACAGCCTCT AAATGATGAG
    GTCGTGCCCT GCAACCAGTC CCAGGGGGAC GGTGCAGCGG CCTGTTCCTG CCAGGACTGT
    GCCGCCTCCT GCCCCGTGAT TGCCCAGCCC CGGGCCCTGG ACCCCACCTT CCGCCTGGGC
    CGGATGGAAG GGAGCCTGGT CCTCATTATC ATCCTCTGTT CTCTCTTTGT CTTGCTCACG
    GCCTTCCTCC TGCGGTCCCG CCTGGCCGAG TGGTGCAGGG GCAAGCGCAA GACGCCCAAG
    CCCAAGGCAA GCATCAACCT GGCCCACAGG CTTAGCCTCT CCACCCACAC CCTCCTCAGC
    CGCTGCTTCC AGTGCTGGGG CACTTGGGTG GCCTCATGGC CACTGACCAT CCTGGCGGTA
    TCTGTGATTG TGGTGGTGGC CCTGGCAGGG GGCCTGGCTT TTATAGAACT GACCACGGAC
    CCCGTGGAGT TGTGGTCAGC CCCCAACAGC CTAGCCCGAC GTGAGAAGGC GTTCCACGAC
    AAGTATTTCG GCCCCTTCTT CCGAACCAGC CAGGTGTTCA TGACAGCACC TCACCGGCCC
    AGCTATAGGT ATGACTCCCT GCTGCTGGGG CCCAAGAACT TCAGCGGGAT CTTGTCCTCG
    GATCTGCTGC TGGAGGTGCT GGAATTGCAG GAGAGGCTGC GGCATCTGCA GGTGTGGTCG
    CCAGAGGAGC AGCGGAACGT CTCCCTGCGG GACACCTGCT ATGCCCCCCT CAACCCACAT
    AACGCCAGTC TGTCCGACTG CTGCGTCAAC AGCCTCCTGC AGTACTTCCA GAACAACCGC
    ACTCAGCTGC TGCTCACAGC CAACCAGACG CTGTCAGGGC AGACCGCCCA GGTGGACTGG
    AGGGACCACT TCCTCTACTG CGCCAATGCC CCCCTCACCT ACAAAGATGG CACGGCCCTG
    GCCCTGAGCT GCATGGCTGA CTATGGGGCA CCCATCTTCC CCTTCCTCGC CGTGGGGGGC
    TACAAAGGAA AGGACTATTC TGAGGCAGAG GCCCTGATCA TGACCTTCTC CCTCAACAAT
    TACCCTCCTG GGGACCCCAA GCTGGCCCAG GCTAAGCTCT GGGAGGCAGC CTTCTTGGAG
    GAGATGCGAG CCTTTCAGCG TCGGACGGCT GGTGTGTTCC AGGTCACATT CATGGCGGAG
    CGTTCCCTGG AGGATGAGAT CAACAGCACC ACGGCCGAGG ACCTGCCCGT CTTTGCTGTT
    AGCTACCTTG TCATCTTCCT GTACATCTCC CTGGCCCTGG GCAGCTACTC CAGCTGGCGT
    CGGGTACCGG TGGACTCCAA GGCCACGCTG GGCCTGGGCG GGGTGGCCGT GGTGCTGGGA
    GCAGTCGTGG CCTCTATGGG CTTCTTCTCC TACCTGGGTG TCCCTTCCTC ACTGGTGATC
    CTTCAAGTGG TGCCCTTCCT GGTGCTTGCC GTAGGGGCCG ACAACATCTT CATCTTCGTT
    CTTGAGTACC AGAGGCTGCC TCGGAGGCCT GGGGAAGAAC GGGAGGCCCA CATCGGCCGA
    GCCCTGGGCA GAGTGGCCCC CAGCATGCTT CTCTGCAGCC TCTCTGAGGC CATCTGCTTC
    TTCTTAGGAG CCCTGACCCC CATGCCAGCT GTACGGACCT TTGCCCTGAC CTCCGGCTTT
    GCAGTGCTTC TTGACTTCCT GCTACAGATG TCAGCCTTTG TGGCCCTGCT CTCCCTCGAC
    AGCAGGAGGC AAGAGGCCTC CCGGATGGAC ATCTGCTGTT GCAAGACAGC CCAGAAGCTG
    CCCCCGCCGA GCCAGGATGA GGGGCTCCTG CTCCGATTCT TCCGCAAGTT CTACGTCCCA
    TTCCTGTTGC ACTGGCTCAC CCGCGTGGTC GTGGTGCTGC TCTTCCTGGC CCTGTTTGCA
    GCGAGCCTCT ATTTCATGTG CTACATTAAT GTGGGGCTGG ACCAGCAGCT GGCTCTGCCC
    AAGGACTCAT ACCTGATAGA CTATTTCCTC TTTATGAACC GCTACTTTGA GGTGGGGGCC
    CCGGTCTATT TTGTTACCAC TGGGGGCTAC AACTTCTCCA GTGAGGAAGG AATGAATGCC
    ATCTGCTCCA GTGCAGGCTG CAACAACTTC TCCTTAACCC AGAAGATCCA GTACGCCACC
    GACTTCCCTG ATATGTCTTA CCTGGCCATC CCTGCCTCCT CCTGGGTGGA CGACTTCATT
    GACTGGCTGA CTTCATCTTC CTGCTGCCGC CTTTATATCT CTGGTCCCAA TAAAGATGAG
    TTCTGCCCCT CGACTGTCAA CTCCCTGGCC TGCTTGAAGA CCTGTGTGAG CCCCACAGCG
    GGCTCTGCCC GGCCATCAGT GGAGCAGTTC CACAAATATC TTCCCTGGTT TCTGAGTGAC
    GAGCCCAACA TCAAATGTCC CAAAGGTGGC CTGGCAGCAT ACAGCACCTC AGTGAATATG
    AGCTCAGATG GCCAGATTTT AGCCTCGCGG TTCATGGCCT ACAACAAGCC GCTGAAGAAC
    TCGCGGGATT TCACGGAGGC TCTGCGGGCA ACTCGGGCAC TGGCAGCCAA CATCACCGCT
    GACCTGCGGA AGGTGCCAGG CACTGACCCA GATTTTGAGG TCTTCCCTTA CTCGGTCACC
    AACGTGTTCT ATGAGCAGTA CCTGACCATA GTCCCCGAGG GGCTCTTTAT GCTCACCATC
    TGCCTGGTGC CCACCTTTGT CGTCTGCTGC TTCCTGCTGG GCATGGACGT CCGCTCCGGC
    CTCCTCAACC TCTTCTCTAT CATCATGATC CTGGTGGACA CTGTGGGCTT CATGATGCTC
    TGGGACATCA GCTACAATGC TGTGTCCCTC ATCAACCTGG TCACGGCGGT GGGCATCTCT
    GTGGAGTTTG TGTCCCATAT CACCCGCTCC TTTGCCATCA GCACCAAGCC CACCCGGCTG
    GAGAGGGCTA AGGAGGCCAC CATCTCCATG GGCAGTGCGG TGTTTGCAGG AGTAGCCATG
    ACCAACCTGC CTGGCATCCT TGTCCTGGGC CTGGCCAAGG CGCAGCTCAT CCAGATCTTT
    TTCTTCCGTC TCAACCTCCT CATCACTGTG CTGGGCCTGC TGCATGGCCT GGTCTTCCTG
    CCCGTCATCC TCAGCTACCT GGGGCCTGAT GTCAACCCAG CTCTGGTGCA GCAGCAGAAG
    CAGCAGGAGG AGGCAGCTGC GGCCAAGGAG ACCTCTTGCT CAAAGCACCC CGCTCAGATG
    AGCACTGATT ATGGTGTCTA CGTCAACTGC AGCTTTGAAC ATCCTGCCAA GAGTGTAGGA
    GGCCTCGAGA GCTCTCCATC AGAGAACAGG CAGAAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa03944
    Clone ID Related Accession (Same CDS sequence) XM_002686890.4 , XM_588051.7 , XM_002686890.5
    Accession Version XM_002686890.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NPC1-like intracellular cholesterol transporter 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002686890.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGAGA CTGGCCTGAA GGGCTGGTTG CTCTGGGCCC TGCTCCTGCA CTCGGCCCAG 
    AGTGAGGTCT ACACACCCAT ACACCAGCCT GGCTACTGTG CCTTCTACGA TGAGTGCGGG
    AAGAACCCGG AGCTGTCCGG AAGCCTGGCT TCGCTGTCCA ATGTGTCCTG CCTGGATAAC
    TCACCTGCTC GCCACATCAC AGGTGACCAC CTGGCCCTCC TACAAAGCAT CTGTCCCCGT
    CTCTACACTG GCGCCAGCAC CACCTACGCC TGCTGCTCCT CCAAGCAGCT GGTGGCCCTG
    GACATGAGCC TGCGGATCAC CAAGGCCCTC CTCACTCGCT GTCCCGCCTG CTCTGACAAC
    TTCGTGAGCC TGCACTGCCA CAACACCTGC AGCCCCAACC AAAGCCTCTT CATCAATGTG
    ACCCGAGTGG TCACACAGGG GGACAGCCAG TCCCAGGCCG TGGTGGCCTA CGAGGCCTTC
    TACCAGCGCA GCTTTGCCGA GCAGACCTAC AACTCCTGCA GCCGTGTGCG CATCCCCGCA
    GCTGCCACAC TGGCCGTGGG CTCCATGTGT GGCGTCTATG GCTCTGCCCT CTGCAACGCC
    CAGCGCTGGC TCAATTTCCA GGGGGACACG AGCAATGGCC TGGCACCCCT GGACATCACC
    TTCCACCTGT GGGAGCCTAG CCAGGCCGAG GGGAGCACGA TACAGCCTCT AAATGATGAG
    GTCGTGCCCT GCAACCAGTC CCAGGGGGAC GGTGCAGCGG CCTGTTCCTG CCAGGACTGT
    GCCGCCTCCT GCCCCGTGAT TGCCCAGCCC CGGGCCCTGG ACCCCACCTT CCGCCTGGGC
    CGGATGGAAG GGAGCCTGGT CCTCATTATC ATCCTCTGTT CTCTCTTTGT CTTGCTCACG
    GCCTTCCTCC TGCGGTCCCG CCTGGCCGAG TGGTGCAGGG GCAAGCGCAA GACGCCCAAG
    CCCAAGGCAA GCATCAACCT GGCCCACAGG CTTAGCCTCT CCACCCACAC CCTCCTCAGC
    CGCTGCTTCC AGTGCTGGGG CACTTGGGTG GCCTCATGGC CACTGACCAT CCTGGCGGTA
    TCTGTGATTG TGGTGGTGGC CCTGGCAGGG GGCCTGGCTT TTATAGAACT GACCACGGAC
    CCCGTGGAGT TGTGGTCAGC CCCCAACAGC CTAGCCCGAC GTGAGAAGGC GTTCCACGAC
    AAGTATTTCG GCCCCTTCTT CCGAACCAGC CAGGTGTTCA TGACAGCACC TCACCGGCCC
    AGCTATAGGT ATGACTCCCT GCTGCTGGGG CCCAAGAACT TCAGCGGGAT CTTGTCCTCG
    GATCTGCTGC TGGAGGTGCT GGAATTGCAG GAGAGGCTGC GGCATCTGCA GGTGTGGTCG
    CCAGAGGAGC AGCGGAACGT CTCCCTGCGG GACACCTGCT ATGCCCCCCT CAACCCACAT
    AACGCCAGTC TGTCCGACTG CTGCGTCAAC AGCCTCCTGC AGTACTTCCA GAACAACCGC
    ACTCAGCTGC TGCTCACAGC CAACCAGACG CTGTCAGGGC AGACCGCCCA GGTGGACTGG
    AGGGACCACT TCCTCTACTG CGCCAATGCC CCCCTCACCT ACAAAGATGG CACGGCCCTG
    GCCCTGAGCT GCATGGCTGA CTATGGGGCA CCCATCTTCC CCTTCCTCGC CGTGGGGGGC
    TACAAAGGAA AGGACTATTC TGAGGCAGAG GCCCTGATCA TGACCTTCTC CCTCAACAAT
    TACCCTCCTG GGGACCCCAA GCTGGCCCAG GCTAAGCTCT GGGAGGCAGC CTTCTTGGAG
    GAGATGCGAG CCTTTCAGCG TCGGACGGCT GGTGTGTTCC AGGTCACATT CATGGCGGAG
    CGTTCCCTGG AGGATGAGAT CAACAGCACC ACGGCCGAGG ACCTGCCCGT CTTTGCTGTT
    AGCTACCTTG TCATCTTCCT GTACATCTCC CTGGCCCTGG GCAGCTACTC CAGCTGGCGT
    CGGGTACCGG TGGACTCCAA GGCCACGCTG GGCCTGGGCG GGGTGGCCGT GGTGCTGGGA
    GCAGTCGTGG CCTCTATGGG CTTCTTCTCC TACCTGGGTG TCCCTTCCTC ACTGGTGATC
    CTTCAAGTGG TGCCCTTCCT GGTGCTTGCC GTAGGGGCCG ACAACATCTT CATCTTCGTT
    CTTGAGTACC AGAGGCTGCC TCGGAGGCCT GGGGAAGAAC GGGAGGCCCA CATCGGCCGA
    GCCCTGGGCA GAGTGGCCCC CAGCATGCTT CTCTGCAGCC TCTCTGAGGC CATCTGCTTC
    TTCTTAGGAG CCCTGACCCC CATGCCAGCT GTACGGACCT TTGCCCTGAC CTCCGGCTTT
    GCAGTGCTTC TTGACTTCCT GCTACAGATG TCAGCCTTTG TGGCCCTGCT CTCCCTCGAC
    AGCAGGAGGC AAGAGGCCTC CCGGATGGAC ATCTGCTGTT GCAAGACAGC CCAGAAGCTG
    CCCCCGCCGA GCCAGGATGA GGGGCTCCTG CTCCGATTCT TCCGCAAGTT CTACGTCCCA
    TTCCTGTTGC ACTGGCTCAC CCGCGTGGTC GTGGTGCTGC TCTTCCTGGC CCTGTTTGCA
    GCGAGCCTCT ATTTCATGTG CTACATTAAT GTGGGGCTGG ACCAGCAGCT GGCTCTGCCC
    AAGGACTCAT ACCTGATAGA CTATTTCCTC TTTATGAACC GCTACTTTGA GGTGGGGGCC
    CCGGTCTATT TTGTTACCAC TGGGGGCTAC AACTTCTCCA GTGAGGAAGG AATGAATGCC
    ATCTGCTCCA GTGCAGGCTG CAACAACTTC TCCTTAACCC AGAAGATCCA GTACGCCACC
    GACTTCCCTG ATATGTCTTA CCTGGCCATC CCTGCCTCCT CCTGGGTGGA CGACTTCATT
    GACTGGCTGA CTTCATCTTC CTGCTGCCGC CTTTATATCT CTGGTCCCAA TAAAGATGAG
    TTCTGCCCCT CGACTGTCAA CTCCCTGGCC TGCTTGAAGA CCTGTGTGAG CCCCACAGCG
    GGCTCTGCCC GGCCATCAGT GGAGCAGTTC CACAAATATC TTCCCTGGTT TCTGAGTGAC
    GAGCCCAACA TCAAATGTCC CAAAGGTGGC CTGGCAGCAT ACAGCACCTC AGTGAATATG
    AGCTCAGATG GCCAGATTTT AGCCTCGCGG TTCATGGCCT ACAACAAGCC GCTGAAGAAC
    TCGCGGGATT TCACGGAGGC TCTGCGGGCA ACTCGGGCAC TGGCAGCCAA CATCACCGCT
    GACCTGCGGA AGGTGCCAGG CACTGACCCA GATTTTGAGG TCTTCCCTTA CTCGGTCACC
    AACGTGTTCT ATGAGCAGTA CCTGACCATA GTCCCCGAGG GGCTCTTTAT GCTCACCATC
    TGCCTGGTGC CCACCTTTGT CGTCTGCTGC TTCCTGCTGG GCATGGACGT CCGCTCCGGC
    CTCCTCAACC TCTTCTCTAT CATCATGATC CTGGTGGACA CTGTGGGCTT CATGATGCTC
    TGGGACATCA GCTACAATGC TGTGTCCCTC ATCAACCTGG TCACGGCGGT GGGCATCTCT
    GTGGAGTTTG TGTCCCATAT CACCCGCTCC TTTGCCATCA GCACCAAGCC CACCCGGCTG
    GAGAGGGCTA AGGAGGCCAC CATCTCCATG GGCAGTGCGG TGTTTGCAGG AGTAGCCATG
    ACCAACCTGC CTGGCATCCT TGTCCTGGGC CTGGCCAAGG CGCAGCTCAT CCAGATCTTT
    TTCTTCCGTC TCAACCTCCT CATCACTGTG CTGGGCCTGC TGCATGGCCT GGTCTTCCTG
    CCCGTCATCC TCAGCTACCT GGGGCCTGAT GTCAACCCAG CTCTGGTGCA GCAGCAGAAG
    CAGCAGGAGG AGGCAGCTGC GGCCAAGGAG ACCTCTTGCT CAAAGCACCC CGCTCAGATG
    AGCACTGATT ATGGTGTCTA CGTCAACTGC AGCTTTGAAC ATCCTGCCAA GAGTGTAGGA
    GGCCTCGAGA GCTCTCCATC AGAGAACAGG CAGAAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002686936.1
    CDS152..4153
    Translation

    Target ORF information:

    RefSeq Version XM_002686890.5
    Organism Bos taurus(cattle)
    Definition Bos taurus NPC1 like intracellular cholesterol transporter 1 (NPC1L1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002686890.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGTGGAGA CTGGCCTGAA GGGCTGGTTG CTCTGGGCCC TGCTCCTGCA CTCGGCCCAG 
    AGTGAGGTCT ACACACCCAT ACACCAGCCT GGCTACTGTG CCTTCTACGA TGAGTGCGGG
    AAGAACCCGG AGCTGTCCGG AAGCCTGGCT TCGCTGTCCA ATGTGTCCTG CCTGGATAAC
    TCACCTGCTC GCCACATCAC AGGTGACCAC CTGGCCCTCC TACAAAGCAT CTGTCCCCGT
    CTCTACACTG GCGCCAGCAC CACCTACGCC TGCTGCTCCT CCAAGCAGCT GGTGGCCCTG
    GACATGAGCC TGCGGATCAC CAAGGCCCTC CTCACTCGCT GTCCCGCCTG CTCTGACAAC
    TTCGTGAGCC TGCACTGCCA CAACACCTGC AGCCCCAACC AAAGCCTCTT CATCAATGTG
    ACCCGAGTGG TCACACAGGG GGACAGCCAG TCCCAGGCCG TGGTGGCCTA CGAGGCCTTC
    TACCAGCGCA GCTTTGCCGA GCAGACCTAC AACTCCTGCA GCCGTGTGCG CATCCCCGCA
    GCTGCCACAC TGGCCGTGGG CTCCATGTGT GGCGTCTATG GCTCTGCCCT CTGCAACGCC
    CAGCGCTGGC TCAATTTCCA GGGGGACACG AGCAATGGCC TGGCACCCCT GGACATCACC
    TTCCACCTGT GGGAGCCTAG CCAGGCCGAG GGGAGCACGA TACAGCCTCT AAATGATGAG
    GTCGTGCCCT GCAACCAGTC CCAGGGGGAC GGTGCAGCGG CCTGTTCCTG CCAGGACTGT
    GCCGCCTCCT GCCCCGTGAT TGCCCAGCCC CGGGCCCTGG ACCCCACCTT CCGCCTGGGC
    CGGATGGAAG GGAGCCTGGT CCTCATTATC ATCCTCTGTT CTCTCTTTGT CTTGCTCACG
    GCCTTCCTCC TGCGGTCCCG CCTGGCCGAG TGGTGCAGGG GCAAGCGCAA GACGCCCAAG
    CCCAAGGCAA GCATCAACCT GGCCCACAGG CTTAGCCTCT CCACCCACAC CCTCCTCAGC
    CGCTGCTTCC AGTGCTGGGG CACTTGGGTG GCCTCATGGC CACTGACCAT CCTGGCGGTA
    TCTGTGATTG TGGTGGTGGC CCTGGCAGGG GGCCTGGCTT TTATAGAACT GACCACGGAC
    CCCGTGGAGT TGTGGTCAGC CCCCAACAGC CTAGCCCGAC GTGAGAAGGC GTTCCACGAC
    AAGTATTTCG GCCCCTTCTT CCGAACCAGC CAGGTGTTCA TGACAGCACC TCACCGGCCC
    AGCTATAGGT ATGACTCCCT GCTGCTGGGG CCCAAGAACT TCAGCGGGAT CTTGTCCTCG
    GATCTGCTGC TGGAGGTGCT GGAATTGCAG GAGAGGCTGC GGCATCTGCA GGTGTGGTCG
    CCAGAGGAGC AGCGGAACGT CTCCCTGCGG GACACCTGCT ATGCCCCCCT CAACCCACAT
    AACGCCAGTC TGTCCGACTG CTGCGTCAAC AGCCTCCTGC AGTACTTCCA GAACAACCGC
    ACTCAGCTGC TGCTCACAGC CAACCAGACG CTGTCAGGGC AGACCGCCCA GGTGGACTGG
    AGGGACCACT TCCTCTACTG CGCCAATGCC CCCCTCACCT ACAAAGATGG CACGGCCCTG
    GCCCTGAGCT GCATGGCTGA CTATGGGGCA CCCATCTTCC CCTTCCTCGC CGTGGGGGGC
    TACAAAGGAA AGGACTATTC TGAGGCAGAG GCCCTGATCA TGACCTTCTC CCTCAACAAT
    TACCCTCCTG GGGACCCCAA GCTGGCCCAG GCTAAGCTCT GGGAGGCAGC CTTCTTGGAG
    GAGATGCGAG CCTTTCAGCG TCGGACGGCT GGTGTGTTCC AGGTCACATT CATGGCGGAG
    CGTTCCCTGG AGGATGAGAT CAACAGCACC ACGGCCGAGG ACCTGCCCGT CTTTGCTGTT
    AGCTACCTTG TCATCTTCCT GTACATCTCC CTGGCCCTGG GCAGCTACTC CAGCTGGCGT
    CGGGTACCGG TGGACTCCAA GGCCACGCTG GGCCTGGGCG GGGTGGCCGT GGTGCTGGGA
    GCAGTCGTGG CCTCTATGGG CTTCTTCTCC TACCTGGGTG TCCCTTCCTC ACTGGTGATC
    CTTCAAGTGG TGCCCTTCCT GGTGCTTGCC GTAGGGGCCG ACAACATCTT CATCTTCGTT
    CTTGAGTACC AGAGGCTGCC TCGGAGGCCT GGGGAAGAAC GGGAGGCCCA CATCGGCCGA
    GCCCTGGGCA GAGTGGCCCC CAGCATGCTT CTCTGCAGCC TCTCTGAGGC CATCTGCTTC
    TTCTTAGGAG CCCTGACCCC CATGCCAGCT GTACGGACCT TTGCCCTGAC CTCCGGCTTT
    GCAGTGCTTC TTGACTTCCT GCTACAGATG TCAGCCTTTG TGGCCCTGCT CTCCCTCGAC
    AGCAGGAGGC AAGAGGCCTC CCGGATGGAC ATCTGCTGTT GCAAGACAGC CCAGAAGCTG
    CCCCCGCCGA GCCAGGATGA GGGGCTCCTG CTCCGATTCT TCCGCAAGTT CTACGTCCCA
    TTCCTGTTGC ACTGGCTCAC CCGCGTGGTC GTGGTGCTGC TCTTCCTGGC CCTGTTTGCA
    GCGAGCCTCT ATTTCATGTG CTACATTAAT GTGGGGCTGG ACCAGCAGCT GGCTCTGCCC
    AAGGACTCAT ACCTGATAGA CTATTTCCTC TTTATGAACC GCTACTTTGA GGTGGGGGCC
    CCGGTCTATT TTGTTACCAC TGGGGGCTAC AACTTCTCCA GTGAGGAAGG AATGAATGCC
    ATCTGCTCCA GTGCAGGCTG CAACAACTTC TCCTTAACCC AGAAGATCCA GTACGCCACC
    GACTTCCCTG ATATGTCTTA CCTGGCCATC CCTGCCTCCT CCTGGGTGGA CGACTTCATT
    GACTGGCTGA CTTCATCTTC CTGCTGCCGC CTTTATATCT CTGGTCCCAA TAAAGATGAG
    TTCTGCCCCT CGACTGTCAA CTCCCTGGCC TGCTTGAAGA CCTGTGTGAG CCCCACAGCG
    GGCTCTGCCC GGCCATCAGT GGAGCAGTTC CACAAATATC TTCCCTGGTT TCTGAGTGAC
    GAGCCCAACA TCAAATGTCC CAAAGGTGGC CTGGCAGCAT ACAGCACCTC AGTGAATATG
    AGCTCAGATG GCCAGATTTT AGCCTCGCGG TTCATGGCCT ACAACAAGCC GCTGAAGAAC
    TCGCGGGATT TCACGGAGGC TCTGCGGGCA ACTCGGGCAC TGGCAGCCAA CATCACCGCT
    GACCTGCGGA AGGTGCCAGG CACTGACCCA GATTTTGAGG TCTTCCCTTA CTCGGTCACC
    AACGTGTTCT ATGAGCAGTA CCTGACCATA GTCCCCGAGG GGCTCTTTAT GCTCACCATC
    TGCCTGGTGC CCACCTTTGT CGTCTGCTGC TTCCTGCTGG GCATGGACGT CCGCTCCGGC
    CTCCTCAACC TCTTCTCTAT CATCATGATC CTGGTGGACA CTGTGGGCTT CATGATGCTC
    TGGGACATCA GCTACAATGC TGTGTCCCTC ATCAACCTGG TCACGGCGGT GGGCATCTCT
    GTGGAGTTTG TGTCCCATAT CACCCGCTCC TTTGCCATCA GCACCAAGCC CACCCGGCTG
    GAGAGGGCTA AGGAGGCCAC CATCTCCATG GGCAGTGCGG TGTTTGCAGG AGTAGCCATG
    ACCAACCTGC CTGGCATCCT TGTCCTGGGC CTGGCCAAGG CGCAGCTCAT CCAGATCTTT
    TTCTTCCGTC TCAACCTCCT CATCACTGTG CTGGGCCTGC TGCATGGCCT GGTCTTCCTG
    CCCGTCATCC TCAGCTACCT GGGGCCTGAT GTCAACCCAG CTCTGGTGCA GCAGCAGAAG
    CAGCAGGAGG AGGCAGCTGC GGCCAAGGAG ACCTCTTGCT CAAAGCACCC CGCTCAGATG
    AGCACTGATT ATGGTGTCTA CGTCAACTGC AGCTTTGAAC ATCCTGCCAA GAGTGTAGGA
    GGCCTCGAGA GCTCTCCATC AGAGAACAGG CAGAAGTTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.