FGD6 cDNA ORF clone, Bos taurus(cattle)

The following FGD6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FGD6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa234730 XM_024992408.1
Latest version!
Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa54319 XM_010827606.2
Latest version!
Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa54319 XM_010804840.2
Latest version!
Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa04187 XM_005206128.4
Latest version!
Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa04187 XM_005198220.3
Latest version!
Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa04188 XM_587549.9
Latest version!
Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa04188 XM_002687205.6
Latest version!
Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa04188 XM_002687205.5 Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa04187 XM_005206128.3 Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa234730
    Clone ID Related Accession (Same CDS sequence) XM_024992408.1
    Accession Version XM_024992408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4032bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA TGTCCTCCGG AGTGGCAGCA GCACCACCAT
    GGAACTCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848176.1
    CDS310..4341
    Translation

    Target ORF information:

    RefSeq Version XM_024992408.1
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA TGTCCTCCGG AGTGGCAGCA GCACCACCAT
    GGAACTCACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54319
    Clone ID Related Accession (Same CDS sequence) XM_010804840.2 , XM_010827606.2
    Accession Version XM_010804840.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3681bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGATG GCTGTAATTC CCACAGACAA TTCAGCATTG AATTTGCAGA TTTGTCACCT 
    TCCCTGTCCA GCTTTGAAAA CGTTTCTAAT CATCACCCTT GCCACCTACA ACTTCCTAGC
    ACTGAATTTC AAAATTTTGA AACTAATCAG AATGACAGTG AAAAAAGCAA CACTTGCTAT
    CATTCATCTG AACAAGAAGC TCTAGAAAAT GGAAAAAGGA GTCCTTTCTT ATCTTCAGAT
    GGAGATAGTA AGAAATCAGA AGCCAGAGAC CTTGGTCCCT TGGAAATCCA CTTAGTGCCA
    TGTACTCCCA AATTTCCAAC TCCCAAGCCC AGAAAAACAC ATGTTGCACG CCTCTTACGC
    CAGAGACATA TAGATACTTC TAGTGAAAGT ACTGAAGAAC CAGAGGATGT AGACAGCTTG
    CCTCATGTTT TCGAAGATAG TTTGAAAAAC AATAAAATCA GTGTCTTTCA TCAGACTGCT
    TTGTGTAACC AGGAACAGGT GGACAAAGTG AAGCCAGAAA ATAAAGGTGA ATTGAATGTG
    GACCTCACTG GTGACAGACA AAACTCGGTC TATTCACAGA AAGCCTTGCG TCCTGAAACA
    TTTTCCTCTG AAAAAACGGC ACCTCCTTTA GAAACAGACT CTAACTTGAG TTCTGACGAC
    AAGTCAGGAG ATAGTTCTGG TATGTCACTT GCCGAGGACA AAGGAATCAG TTTTATAAGA
    TGCAGTACTC TATCTATGAG CCTGCCCAAG CAGCTCAAAT TAACCTGCAG CGAACATTTG
    CCTACTGCCT CTAACCCAGG AGTGTCTGCC CCTCAAATGC AAAAGGAATC TATGATAAAA
    GGTGAAAGTT CCTCAAAAGT TGTCCCCAAA AAACCTCAAA GACACAGTTT GCCTGCTGCC
    GGAGTGCTTA AAAAGGCTGC CTCTGAGGAG CTTTTGGAGA AAAGTTCTTA TCCCTCAAAT
    GAAGAAAAAG ATGCAGAGAA GGGTCTAGAA AGAGATCACG TGCAGCATTT ATGTGCCCCA
    AACAATGGTA TGTCATCCTC CTTTGATATG CCTAAACGGG CTTCAGAGAA GCCAGTGTGG
    AAATTACCTC ATCCCATTAT CCCCTTTTCA GGGAACCCAG AATCCTTAAA GTCTGTCACC
    ATATCCTCAA ACAGTGAGAC TCCCACACCC CTGACCAAGC CTAGAGCAAA ATCATTATCT
    GCTATGGATG TGGAAAGGTG CACTAAGCCA TGCAAAGACT CTCACAAGAA GAACTCTTTA
    AAGAAGTTGC TCAATATGAA GCTGTCCATC TGTTTCATGA AGAGTGACTT CCAGAAATTT
    TGGTCCAAGC ATAGCCATCT TGGAGACACA ACCTCAGGCA GCCTGGCAGG TGGGGAGCAA
    AGAGGGAGTG AGAGTGATTG GCATGGCCTG TTGGTAGGAG AAGAGAAGAG AAGTAAACCC
    ATCAAGGCAT ATTCGGCAGA TAACTATAGC CTGGAGTCCC ACAAGAAGAG GAAGAAGTTG
    CGGGGCCACA CCAATGCAGC TAATGGACCA AGAGCCGAGT CTCTGGATGA CCAAATGCTG
    TCCAGGGAGT CGTCCTTGCA GGCACCTTGC AAATCTGTTA CCAGCGACTG TGTACCAGAG
    TATGAAAATA TACGGCATTA TGAGGAAATA CCAGAGTATG AGAACTTGCC CTTTGTTATG
    GCTATGGGGA AAACTCCCGA GTTGGAACGA CGGAACTCCA GCAGCGTGGA GGACACTGAT
    GCAAATGTGT ATGAGGTACA GGAGCCGTAT GAAGCTCCAG ACGGCCAGCT CCAACTCGGA
    CCCAGACACC AGCATTCCAG CAGTTCTGGA GCATCCCAGG AGGGACAAAG TGATCTGGAC
    CTTGGTCTTG GTGACCTTCC TTCAGATGAA GAGGAAGTCA TCAACAGTTC AGATGAAGAT
    GATGTCAGCT CTGCTTCTAG TAAAGGGGAG CCTGATCTGC TGGACGATAA ACAGGGTGAA
    GATACTGGAA TGAAATGTAA AGTCCATCAT ATTGCCAAAG AGATCATGAG CTCAGAAAAA
    GTGTTTGTGG ATGTGTTAAA ACTTTTGCAT ATTGATTTCC GGGATGCAGT AGCCCATGCT
    TCCAGGCAAC TTGGGAAACC AGTGATTGAG GACCGGATCC TAAACCAGAT CCTGTACTAC
    TTGCCTCAGC TGTATGAGCT CAATCGGGAT CTCCTGAAGG AGCTGGAGGA AAGAATGTCA
    AACTGGACGG AACAACAAAG AATTGCTGAT ATCTTTGTAA AGAAGGGACC ATATCTAAAA
    ATGTATTCCA CATACATCAA AGAATTTGAT AAGAATATAG CTTTGTTGGA TGAGCAGTGC
    AAGAAAAACC CAGGTTTTGC TGCTGTTGTT AGAGAATTTG AGATGAGCCC ACGCTGTGCT
    AATCTGGCCC TCAAGCACTA CCTGCTCAAG CCGGTTCAGA GGATTCCCCA GTACAGGCTG
    CTGCTAACAG ATTATTTGAA AAATCTCTTA GAAGACTCTG GAGATTACAG AGACACTCAA
    GATGCCCTTG CTGTTGTTAT AGAGGTAGCC AACCACGCCA ATGACACCAT GAAGCAAGGA
    GACAACTTCC AGAAACTTAT GCAAATTCAG TACAGCTTAA ATGGACACCA TGAAATAGTT
    CAGCCTGGAC GGGTTTTTCT CAAAGAAGGA ATTCTGATGA AGCTATCTCG GAAAGTCATG
    CAGCCCCGAA TGTTTTTCCT GTTTAATGAT GCCCTGTTGT ATACAACACC AGTGCAGTCT
    GGGATGTACA AACTGAACAA CATGCTATCT TTGGCTGGAA TGAAGGTCAG AAAACCCACC
    CAAGAAGCAT ATCAAAATGA ACTTAAGATC GAAAGTGTAG AACGTTCCTT CATCCTCTCA
    GCCAGTTCTG CCCCAGAAAG GGATGAATGG CTCGAAGCCA TTTCCAGGTC AATAGAAGAG
    TATGCCAAGA AAAGAATCAC ATTCTGTCCT AGTCGGAGTC TTGATGAGGC TGACTCAGAA
    GATAAAGACG AGGTTAGTCC TCTTGGATCT AAGGCTCCCA TCTGGATTCC TGATACCAGG
    GCCACCATGT GTATGATCTG CACCAGTGAA TTTACTCTCA CCTGGAGACG ACATCACTGC
    CGGGCCTGCG GAAAGATTGT ATGCCAAGCT TGTTCATCAA ATAAGTATGG CTTAGATTAC
    CTGAAAAATC AACCAGCAAG AGTATGTGAA CACTGTTTCC AAGAACTGCA GAAATTAGAT
    CACCAGCACT CTCCTAAGTT TGGATCCCCT GGAAATCACA AATCTCCTTC CAGCGCCTTA
    TCATCAGTCT TACATAGTAT TCCATCAGGG AGGAAACAGA AAAAAATCCC AGCTGCTCTG
    AAAGAAGTAT CAGCAAACAC AGAAGATTCC TCTATGAGTG GCTACTTGTA CAGATCAAAG
    GGCAATAAAA AACCTTGGAA ACACTTGTGG TTTGTCATAA AAAATAAAGT ACTGTATACA
    TATGCTGCAA GTGAGGATGT GGCAGCTTTG GAGAGTCAAC CTTTACTAGG ATTCACAGTT
    ACTCAAGTCA AAGATGAGAA TTCAGAGTCT AGAGTGTTTC AGTTACTGCA CAAAAACATG
    CTATTTTATG TATTCAAAGC AGATGACGCT CACTCAGCTC AGAAGTGGAT AGAAGCATTT
    CAAGAAGGCA CAATACTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803142.1
    CDS138..3818
    Translation

    Target ORF information:

    RefSeq Version XM_010804840.2
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804840.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGATG GCTGTAATTC CCACAGACAA TTCAGCATTG AATTTGCAGA TTTGTCACCT 
    TCCCTGTCCA GCTTTGAAAA CGTTTCTAAT CATCACCCTT GCCACCTACA ACTTCCTAGC
    ACTGAATTTC AAAATTTTGA AACTAATCAG AATGACAGTG AAAAAAGCAA CACTTGCTAT
    CATTCATCTG AACAAGAAGC TCTAGAAAAT GGAAAAAGGA GTCCTTTCTT ATCTTCAGAT
    GGAGATAGTA AGAAATCAGA AGCCAGAGAC CTTGGTCCCT TGGAAATCCA CTTAGTGCCA
    TGTACTCCCA AATTTCCAAC TCCCAAGCCC AGAAAAACAC ATGTTGCACG CCTCTTACGC
    CAGAGACATA TAGATACTTC TAGTGAAAGT ACTGAAGAAC CAGAGGATGT AGACAGCTTG
    CCTCATGTTT TCGAAGATAG TTTGAAAAAC AATAAAATCA GTGTCTTTCA TCAGACTGCT
    TTGTGTAACC AGGAACAGGT GGACAAAGTG AAGCCAGAAA ATAAAGGTGA ATTGAATGTG
    GACCTCACTG GTGACAGACA AAACTCGGTC TATTCACAGA AAGCCTTGCG TCCTGAAACA
    TTTTCCTCTG AAAAAACGGC ACCTCCTTTA GAAACAGACT CTAACTTGAG TTCTGACGAC
    AAGTCAGGAG ATAGTTCTGG TATGTCACTT GCCGAGGACA AAGGAATCAG TTTTATAAGA
    TGCAGTACTC TATCTATGAG CCTGCCCAAG CAGCTCAAAT TAACCTGCAG CGAACATTTG
    CCTACTGCCT CTAACCCAGG AGTGTCTGCC CCTCAAATGC AAAAGGAATC TATGATAAAA
    GGTGAAAGTT CCTCAAAAGT TGTCCCCAAA AAACCTCAAA GACACAGTTT GCCTGCTGCC
    GGAGTGCTTA AAAAGGCTGC CTCTGAGGAG CTTTTGGAGA AAAGTTCTTA TCCCTCAAAT
    GAAGAAAAAG ATGCAGAGAA GGGTCTAGAA AGAGATCACG TGCAGCATTT ATGTGCCCCA
    AACAATGGTA TGTCATCCTC CTTTGATATG CCTAAACGGG CTTCAGAGAA GCCAGTGTGG
    AAATTACCTC ATCCCATTAT CCCCTTTTCA GGGAACCCAG AATCCTTAAA GTCTGTCACC
    ATATCCTCAA ACAGTGAGAC TCCCACACCC CTGACCAAGC CTAGAGCAAA ATCATTATCT
    GCTATGGATG TGGAAAGGTG CACTAAGCCA TGCAAAGACT CTCACAAGAA GAACTCTTTA
    AAGAAGTTGC TCAATATGAA GCTGTCCATC TGTTTCATGA AGAGTGACTT CCAGAAATTT
    TGGTCCAAGC ATAGCCATCT TGGAGACACA ACCTCAGGCA GCCTGGCAGG TGGGGAGCAA
    AGAGGGAGTG AGAGTGATTG GCATGGCCTG TTGGTAGGAG AAGAGAAGAG AAGTAAACCC
    ATCAAGGCAT ATTCGGCAGA TAACTATAGC CTGGAGTCCC ACAAGAAGAG GAAGAAGTTG
    CGGGGCCACA CCAATGCAGC TAATGGACCA AGAGCCGAGT CTCTGGATGA CCAAATGCTG
    TCCAGGGAGT CGTCCTTGCA GGCACCTTGC AAATCTGTTA CCAGCGACTG TGTACCAGAG
    TATGAAAATA TACGGCATTA TGAGGAAATA CCAGAGTATG AGAACTTGCC CTTTGTTATG
    GCTATGGGGA AAACTCCCGA GTTGGAACGA CGGAACTCCA GCAGCGTGGA GGACACTGAT
    GCAAATGTGT ATGAGGTACA GGAGCCGTAT GAAGCTCCAG ACGGCCAGCT CCAACTCGGA
    CCCAGACACC AGCATTCCAG CAGTTCTGGA GCATCCCAGG AGGGACAAAG TGATCTGGAC
    CTTGGTCTTG GTGACCTTCC TTCAGATGAA GAGGAAGTCA TCAACAGTTC AGATGAAGAT
    GATGTCAGCT CTGCTTCTAG TAAAGGGGAG CCTGATCTGC TGGACGATAA ACAGGGTGAA
    GATACTGGAA TGAAATGTAA AGTCCATCAT ATTGCCAAAG AGATCATGAG CTCAGAAAAA
    GTGTTTGTGG ATGTGTTAAA ACTTTTGCAT ATTGATTTCC GGGATGCAGT AGCCCATGCT
    TCCAGGCAAC TTGGGAAACC AGTGATTGAG GACCGGATCC TAAACCAGAT CCTGTACTAC
    TTGCCTCAGC TGTATGAGCT CAATCGGGAT CTCCTGAAGG AGCTGGAGGA AAGAATGTCA
    AACTGGACGG AACAACAAAG AATTGCTGAT ATCTTTGTAA AGAAGGGACC ATATCTAAAA
    ATGTATTCCA CATACATCAA AGAATTTGAT AAGAATATAG CTTTGTTGGA TGAGCAGTGC
    AAGAAAAACC CAGGTTTTGC TGCTGTTGTT AGAGAATTTG AGATGAGCCC ACGCTGTGCT
    AATCTGGCCC TCAAGCACTA CCTGCTCAAG CCGGTTCAGA GGATTCCCCA GTACAGGCTG
    CTGCTAACAG ATTATTTGAA AAATCTCTTA GAAGACTCTG GAGATTACAG AGACACTCAA
    GATGCCCTTG CTGTTGTTAT AGAGGTAGCC AACCACGCCA ATGACACCAT GAAGCAAGGA
    GACAACTTCC AGAAACTTAT GCAAATTCAG TACAGCTTAA ATGGACACCA TGAAATAGTT
    CAGCCTGGAC GGGTTTTTCT CAAAGAAGGA ATTCTGATGA AGCTATCTCG GAAAGTCATG
    CAGCCCCGAA TGTTTTTCCT GTTTAATGAT GCCCTGTTGT ATACAACACC AGTGCAGTCT
    GGGATGTACA AACTGAACAA CATGCTATCT TTGGCTGGAA TGAAGGTCAG AAAACCCACC
    CAAGAAGCAT ATCAAAATGA ACTTAAGATC GAAAGTGTAG AACGTTCCTT CATCCTCTCA
    GCCAGTTCTG CCCCAGAAAG GGATGAATGG CTCGAAGCCA TTTCCAGGTC AATAGAAGAG
    TATGCCAAGA AAAGAATCAC ATTCTGTCCT AGTCGGAGTC TTGATGAGGC TGACTCAGAA
    GATAAAGACG AGGTTAGTCC TCTTGGATCT AAGGCTCCCA TCTGGATTCC TGATACCAGG
    GCCACCATGT GTATGATCTG CACCAGTGAA TTTACTCTCA CCTGGAGACG ACATCACTGC
    CGGGCCTGCG GAAAGATTGT ATGCCAAGCT TGTTCATCAA ATAAGTATGG CTTAGATTAC
    CTGAAAAATC AACCAGCAAG AGTATGTGAA CACTGTTTCC AAGAACTGCA GAAATTAGAT
    CACCAGCACT CTCCTAAGTT TGGATCCCCT GGAAATCACA AATCTCCTTC CAGCGCCTTA
    TCATCAGTCT TACATAGTAT TCCATCAGGG AGGAAACAGA AAAAAATCCC AGCTGCTCTG
    AAAGAAGTAT CAGCAAACAC AGAAGATTCC TCTATGAGTG GCTACTTGTA CAGATCAAAG
    GGCAATAAAA AACCTTGGAA ACACTTGTGG TTTGTCATAA AAAATAAAGT ACTGTATACA
    TATGCTGCAA GTGAGGATGT GGCAGCTTTG GAGAGTCAAC CTTTACTAGG ATTCACAGTT
    ACTCAAGTCA AAGATGAGAA TTCAGAGTCT AGAGTGTTTC AGTTACTGCA CAAAAACATG
    CTATTTTATG TATTCAAAGC AGATGACGCT CACTCAGCTC AGAAGTGGAT AGAAGCATTT
    CAAGAAGGCA CAATACTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa54319
    Clone ID Related Accession (Same CDS sequence) XM_010804840.2 , XM_010827606.2
    Accession Version XM_010827606.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3681bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644784.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGATG GCTGTAATTC CCACAGACAA TTCAGCATTG AATTTGCAGA TTTGTCACCT 
    TCCCTGTCCA GCTTTGAAAA CGTTTCTAAT CATCACCCTT GCCACCTACA ACTTCCTAGC
    ACTGAATTTC AAAATTTTGA AACTAATCAG AATGACAGTG AAAAAAGCAA CACTTGCTAT
    CATTCATCTG AACAAGAAGC TCTAGAAAAT GGAAAAAGGA GTCCTTTCTT ATCTTCAGAT
    GGAGATAGTA AGAAATCAGA AGCCAGAGAC CTTGGTCCCT TGGAAATCCA CTTAGTGCCA
    TGTACTCCCA AATTTCCAAC TCCCAAGCCC AGAAAAACAC ATGTTGCACG CCTCTTACGC
    CAGAGACATA TAGATACTTC TAGTGAAAGT ACTGAAGAAC CAGAGGATGT AGACAGCTTG
    CCTCATGTTT TCGAAGATAG TTTGAAAAAC AATAAAATCA GTGTCTTTCA TCAGACTGCT
    TTGTGTAACC AGGAACAGGT GGACAAAGTG AAGCCAGAAA ATAAAGGTGA ATTGAATGTG
    GACCTCACTG GTGACAGACA AAACTCGGTC TATTCACAGA AAGCCTTGCG TCCTGAAACA
    TTTTCCTCTG AAAAAACGGC ACCTCCTTTA GAAACAGACT CTAACTTGAG TTCTGACGAC
    AAGTCAGGAG ATAGTTCTGG TATGTCACTT GCCGAGGACA AAGGAATCAG TTTTATAAGA
    TGCAGTACTC TATCTATGAG CCTGCCCAAG CAGCTCAAAT TAACCTGCAG CGAACATTTG
    CCTACTGCCT CTAACCCAGG AGTGTCTGCC CCTCAAATGC AAAAGGAATC TATGATAAAA
    GGTGAAAGTT CCTCAAAAGT TGTCCCCAAA AAACCTCAAA GACACAGTTT GCCTGCTGCC
    GGAGTGCTTA AAAAGGCTGC CTCTGAGGAG CTTTTGGAGA AAAGTTCTTA TCCCTCAAAT
    GAAGAAAAAG ATGCAGAGAA GGGTCTAGAA AGAGATCACG TGCAGCATTT ATGTGCCCCA
    AACAATGGTA TGTCATCCTC CTTTGATATG CCTAAACGGG CTTCAGAGAA GCCAGTGTGG
    AAATTACCTC ATCCCATTAT CCCCTTTTCA GGGAACCCAG AATCCTTAAA GTCTGTCACC
    ATATCCTCAA ACAGTGAGAC TCCCACACCC CTGACCAAGC CTAGAGCAAA ATCATTATCT
    GCTATGGATG TGGAAAGGTG CACTAAGCCA TGCAAAGACT CTCACAAGAA GAACTCTTTA
    AAGAAGTTGC TCAATATGAA GCTGTCCATC TGTTTCATGA AGAGTGACTT CCAGAAATTT
    TGGTCCAAGC ATAGCCATCT TGGAGACACA ACCTCAGGCA GCCTGGCAGG TGGGGAGCAA
    AGAGGGAGTG AGAGTGATTG GCATGGCCTG TTGGTAGGAG AAGAGAAGAG AAGTAAACCC
    ATCAAGGCAT ATTCGGCAGA TAACTATAGC CTGGAGTCCC ACAAGAAGAG GAAGAAGTTG
    CGGGGCCACA CCAATGCAGC TAATGGACCA AGAGCCGAGT CTCTGGATGA CCAAATGCTG
    TCCAGGGAGT CGTCCTTGCA GGCACCTTGC AAATCTGTTA CCAGCGACTG TGTACCAGAG
    TATGAAAATA TACGGCATTA TGAGGAAATA CCAGAGTATG AGAACTTGCC CTTTGTTATG
    GCTATGGGGA AAACTCCCGA GTTGGAACGA CGGAACTCCA GCAGCGTGGA GGACACTGAT
    GCAAATGTGT ATGAGGTACA GGAGCCGTAT GAAGCTCCAG ACGGCCAGCT CCAACTCGGA
    CCCAGACACC AGCATTCCAG CAGTTCTGGA GCATCCCAGG AGGGACAAAG TGATCTGGAC
    CTTGGTCTTG GTGACCTTCC TTCAGATGAA GAGGAAGTCA TCAACAGTTC AGATGAAGAT
    GATGTCAGCT CTGCTTCTAG TAAAGGGGAG CCTGATCTGC TGGACGATAA ACAGGGTGAA
    GATACTGGAA TGAAATGTAA AGTCCATCAT ATTGCCAAAG AGATCATGAG CTCAGAAAAA
    GTGTTTGTGG ATGTGTTAAA ACTTTTGCAT ATTGATTTCC GGGATGCAGT AGCCCATGCT
    TCCAGGCAAC TTGGGAAACC AGTGATTGAG GACCGGATCC TAAACCAGAT CCTGTACTAC
    TTGCCTCAGC TGTATGAGCT CAATCGGGAT CTCCTGAAGG AGCTGGAGGA AAGAATGTCA
    AACTGGACGG AACAACAAAG AATTGCTGAT ATCTTTGTAA AGAAGGGACC ATATCTAAAA
    ATGTATTCCA CATACATCAA AGAATTTGAT AAGAATATAG CTTTGTTGGA TGAGCAGTGC
    AAGAAAAACC CAGGTTTTGC TGCTGTTGTT AGAGAATTTG AGATGAGCCC ACGCTGTGCT
    AATCTGGCCC TCAAGCACTA CCTGCTCAAG CCGGTTCAGA GGATTCCCCA GTACAGGCTG
    CTGCTAACAG ATTATTTGAA AAATCTCTTA GAAGACTCTG GAGATTACAG AGACACTCAA
    GATGCCCTTG CTGTTGTTAT AGAGGTAGCC AACCACGCCA ATGACACCAT GAAGCAAGGA
    GACAACTTCC AGAAACTTAT GCAAATTCAG TACAGCTTAA ATGGACACCA TGAAATAGTT
    CAGCCTGGAC GGGTTTTTCT CAAAGAAGGA ATTCTGATGA AGCTATCTCG GAAAGTCATG
    CAGCCCCGAA TGTTTTTCCT GTTTAATGAT GCCCTGTTGT ATACAACACC AGTGCAGTCT
    GGGATGTACA AACTGAACAA CATGCTATCT TTGGCTGGAA TGAAGGTCAG AAAACCCACC
    CAAGAAGCAT ATCAAAATGA ACTTAAGATC GAAAGTGTAG AACGTTCCTT CATCCTCTCA
    GCCAGTTCTG CCCCAGAAAG GGATGAATGG CTCGAAGCCA TTTCCAGGTC AATAGAAGAG
    TATGCCAAGA AAAGAATCAC ATTCTGTCCT AGTCGGAGTC TTGATGAGGC TGACTCAGAA
    GATAAAGACG AGGTTAGTCC TCTTGGATCT AAGGCTCCCA TCTGGATTCC TGATACCAGG
    GCCACCATGT GTATGATCTG CACCAGTGAA TTTACTCTCA CCTGGAGACG ACATCACTGC
    CGGGCCTGCG GAAAGATTGT ATGCCAAGCT TGTTCATCAA ATAAGTATGG CTTAGATTAC
    CTGAAAAATC AACCAGCAAG AGTATGTGAA CACTGTTTCC AAGAACTGCA GAAATTAGAT
    CACCAGCACT CTCCTAAGTT TGGATCCCCT GGAAATCACA AATCTCCTTC CAGCGCCTTA
    TCATCAGTCT TACATAGTAT TCCATCAGGG AGGAAACAGA AAAAAATCCC AGCTGCTCTG
    AAAGAAGTAT CAGCAAACAC AGAAGATTCC TCTATGAGTG GCTACTTGTA CAGATCAAAG
    GGCAATAAAA AACCTTGGAA ACACTTGTGG TTTGTCATAA AAAATAAAGT ACTGTATACA
    TATGCTGCAA GTGAGGATGT GGCAGCTTTG GAGAGTCAAC CTTTACTAGG ATTCACAGTT
    ACTCAAGTCA AAGATGAGAA TTCAGAGTCT AGAGTGTTTC AGTTACTGCA CAAAAACATG
    CTATTTTATG TATTCAAAGC AGATGACGCT CACTCAGCTC AGAAGTGGAT AGAAGCATTT
    CAAGAAGGCA CAATACTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010825908.1
    CDS138..3818
    Translation

    Target ORF information:

    RefSeq Version XM_010827606.2
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010827606.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAGTGATG GCTGTAATTC CCACAGACAA TTCAGCATTG AATTTGCAGA TTTGTCACCT 
    TCCCTGTCCA GCTTTGAAAA CGTTTCTAAT CATCACCCTT GCCACCTACA ACTTCCTAGC
    ACTGAATTTC AAAATTTTGA AACTAATCAG AATGACAGTG AAAAAAGCAA CACTTGCTAT
    CATTCATCTG AACAAGAAGC TCTAGAAAAT GGAAAAAGGA GTCCTTTCTT ATCTTCAGAT
    GGAGATAGTA AGAAATCAGA AGCCAGAGAC CTTGGTCCCT TGGAAATCCA CTTAGTGCCA
    TGTACTCCCA AATTTCCAAC TCCCAAGCCC AGAAAAACAC ATGTTGCACG CCTCTTACGC
    CAGAGACATA TAGATACTTC TAGTGAAAGT ACTGAAGAAC CAGAGGATGT AGACAGCTTG
    CCTCATGTTT TCGAAGATAG TTTGAAAAAC AATAAAATCA GTGTCTTTCA TCAGACTGCT
    TTGTGTAACC AGGAACAGGT GGACAAAGTG AAGCCAGAAA ATAAAGGTGA ATTGAATGTG
    GACCTCACTG GTGACAGACA AAACTCGGTC TATTCACAGA AAGCCTTGCG TCCTGAAACA
    TTTTCCTCTG AAAAAACGGC ACCTCCTTTA GAAACAGACT CTAACTTGAG TTCTGACGAC
    AAGTCAGGAG ATAGTTCTGG TATGTCACTT GCCGAGGACA AAGGAATCAG TTTTATAAGA
    TGCAGTACTC TATCTATGAG CCTGCCCAAG CAGCTCAAAT TAACCTGCAG CGAACATTTG
    CCTACTGCCT CTAACCCAGG AGTGTCTGCC CCTCAAATGC AAAAGGAATC TATGATAAAA
    GGTGAAAGTT CCTCAAAAGT TGTCCCCAAA AAACCTCAAA GACACAGTTT GCCTGCTGCC
    GGAGTGCTTA AAAAGGCTGC CTCTGAGGAG CTTTTGGAGA AAAGTTCTTA TCCCTCAAAT
    GAAGAAAAAG ATGCAGAGAA GGGTCTAGAA AGAGATCACG TGCAGCATTT ATGTGCCCCA
    AACAATGGTA TGTCATCCTC CTTTGATATG CCTAAACGGG CTTCAGAGAA GCCAGTGTGG
    AAATTACCTC ATCCCATTAT CCCCTTTTCA GGGAACCCAG AATCCTTAAA GTCTGTCACC
    ATATCCTCAA ACAGTGAGAC TCCCACACCC CTGACCAAGC CTAGAGCAAA ATCATTATCT
    GCTATGGATG TGGAAAGGTG CACTAAGCCA TGCAAAGACT CTCACAAGAA GAACTCTTTA
    AAGAAGTTGC TCAATATGAA GCTGTCCATC TGTTTCATGA AGAGTGACTT CCAGAAATTT
    TGGTCCAAGC ATAGCCATCT TGGAGACACA ACCTCAGGCA GCCTGGCAGG TGGGGAGCAA
    AGAGGGAGTG AGAGTGATTG GCATGGCCTG TTGGTAGGAG AAGAGAAGAG AAGTAAACCC
    ATCAAGGCAT ATTCGGCAGA TAACTATAGC CTGGAGTCCC ACAAGAAGAG GAAGAAGTTG
    CGGGGCCACA CCAATGCAGC TAATGGACCA AGAGCCGAGT CTCTGGATGA CCAAATGCTG
    TCCAGGGAGT CGTCCTTGCA GGCACCTTGC AAATCTGTTA CCAGCGACTG TGTACCAGAG
    TATGAAAATA TACGGCATTA TGAGGAAATA CCAGAGTATG AGAACTTGCC CTTTGTTATG
    GCTATGGGGA AAACTCCCGA GTTGGAACGA CGGAACTCCA GCAGCGTGGA GGACACTGAT
    GCAAATGTGT ATGAGGTACA GGAGCCGTAT GAAGCTCCAG ACGGCCAGCT CCAACTCGGA
    CCCAGACACC AGCATTCCAG CAGTTCTGGA GCATCCCAGG AGGGACAAAG TGATCTGGAC
    CTTGGTCTTG GTGACCTTCC TTCAGATGAA GAGGAAGTCA TCAACAGTTC AGATGAAGAT
    GATGTCAGCT CTGCTTCTAG TAAAGGGGAG CCTGATCTGC TGGACGATAA ACAGGGTGAA
    GATACTGGAA TGAAATGTAA AGTCCATCAT ATTGCCAAAG AGATCATGAG CTCAGAAAAA
    GTGTTTGTGG ATGTGTTAAA ACTTTTGCAT ATTGATTTCC GGGATGCAGT AGCCCATGCT
    TCCAGGCAAC TTGGGAAACC AGTGATTGAG GACCGGATCC TAAACCAGAT CCTGTACTAC
    TTGCCTCAGC TGTATGAGCT CAATCGGGAT CTCCTGAAGG AGCTGGAGGA AAGAATGTCA
    AACTGGACGG AACAACAAAG AATTGCTGAT ATCTTTGTAA AGAAGGGACC ATATCTAAAA
    ATGTATTCCA CATACATCAA AGAATTTGAT AAGAATATAG CTTTGTTGGA TGAGCAGTGC
    AAGAAAAACC CAGGTTTTGC TGCTGTTGTT AGAGAATTTG AGATGAGCCC ACGCTGTGCT
    AATCTGGCCC TCAAGCACTA CCTGCTCAAG CCGGTTCAGA GGATTCCCCA GTACAGGCTG
    CTGCTAACAG ATTATTTGAA AAATCTCTTA GAAGACTCTG GAGATTACAG AGACACTCAA
    GATGCCCTTG CTGTTGTTAT AGAGGTAGCC AACCACGCCA ATGACACCAT GAAGCAAGGA
    GACAACTTCC AGAAACTTAT GCAAATTCAG TACAGCTTAA ATGGACACCA TGAAATAGTT
    CAGCCTGGAC GGGTTTTTCT CAAAGAAGGA ATTCTGATGA AGCTATCTCG GAAAGTCATG
    CAGCCCCGAA TGTTTTTCCT GTTTAATGAT GCCCTGTTGT ATACAACACC AGTGCAGTCT
    GGGATGTACA AACTGAACAA CATGCTATCT TTGGCTGGAA TGAAGGTCAG AAAACCCACC
    CAAGAAGCAT ATCAAAATGA ACTTAAGATC GAAAGTGTAG AACGTTCCTT CATCCTCTCA
    GCCAGTTCTG CCCCAGAAAG GGATGAATGG CTCGAAGCCA TTTCCAGGTC AATAGAAGAG
    TATGCCAAGA AAAGAATCAC ATTCTGTCCT AGTCGGAGTC TTGATGAGGC TGACTCAGAA
    GATAAAGACG AGGTTAGTCC TCTTGGATCT AAGGCTCCCA TCTGGATTCC TGATACCAGG
    GCCACCATGT GTATGATCTG CACCAGTGAA TTTACTCTCA CCTGGAGACG ACATCACTGC
    CGGGCCTGCG GAAAGATTGT ATGCCAAGCT TGTTCATCAA ATAAGTATGG CTTAGATTAC
    CTGAAAAATC AACCAGCAAG AGTATGTGAA CACTGTTTCC AAGAACTGCA GAAATTAGAT
    CACCAGCACT CTCCTAAGTT TGGATCCCCT GGAAATCACA AATCTCCTTC CAGCGCCTTA
    TCATCAGTCT TACATAGTAT TCCATCAGGG AGGAAACAGA AAAAAATCCC AGCTGCTCTG
    AAAGAAGTAT CAGCAAACAC AGAAGATTCC TCTATGAGTG GCTACTTGTA CAGATCAAAG
    GGCAATAAAA AACCTTGGAA ACACTTGTGG TTTGTCATAA AAAATAAAGT ACTGTATACA
    TATGCTGCAA GTGAGGATGT GGCAGCTTTG GAGAGTCAAC CTTTACTAGG ATTCACAGTT
    ACTCAAGTCA AAGATGAGAA TTCAGAGTCT AGAGTGTTTC AGTTACTGCA CAAAAACATG
    CTATTTTATG TATTCAAAGC AGATGACGCT CACTCAGCTC AGAAGTGGAT AGAAGCATTT
    CAAGAAGGCA CAATACTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04187
    Clone ID Related Accession (Same CDS sequence) XM_005206128.3 , XM_005198220.3 , XM_005206128.4
    Accession Version XM_005206128.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103917.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA GTATCAGCAA ACACAGAAGA TTCCTCTATG
    AGTGGCTACT TGTACAGATC AAAGGGCAAT AAAAAACCTT GGAAACACTT GTGGTTTGTC
    ATAAAAAATA AAGTACTGTA TACATATGCT GCAAGTGAGG ATGTGGCAGC TTTGGAGAGT
    CAACCTTTAC TAGGATTCAC AGTTACTCAA GTCAAAGATG AGAATTCAGA GTCTAGAGTG
    TTTCAGTTAC TGCACAAAAA CATGCTATTT TATGTATTCA AAGCAGATGA CGCTCACTCA
    GCTCAGAAGT GGATAGAAGC ATTTCAAGAA GGCACAATAC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206185.1
    CDS153..4457
    Translation

    Target ORF information:

    RefSeq Version XM_005206128.3
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206128.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA GTATCAGCAA ACACAGAAGA TTCCTCTATG
    AGTGGCTACT TGTACAGATC AAAGGGCAAT AAAAAACCTT GGAAACACTT GTGGTTTGTC
    ATAAAAAATA AAGTACTGTA TACATATGCT GCAAGTGAGG ATGTGGCAGC TTTGGAGAGT
    CAACCTTTAC TAGGATTCAC AGTTACTCAA GTCAAAGATG AGAATTCAGA GTCTAGAGTG
    TTTCAGTTAC TGCACAAAAA CATGCTATTT TATGTATTCA AAGCAGATGA CGCTCACTCA
    GCTCAGAAGT GGATAGAAGC ATTTCAAGAA GGCACAATAC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04187
    Clone ID Related Accession (Same CDS sequence) XM_005206128.3 , XM_005198220.3 , XM_005206128.4
    Accession Version XM_005198220.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644784.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA GTATCAGCAA ACACAGAAGA TTCCTCTATG
    AGTGGCTACT TGTACAGATC AAAGGGCAAT AAAAAACCTT GGAAACACTT GTGGTTTGTC
    ATAAAAAATA AAGTACTGTA TACATATGCT GCAAGTGAGG ATGTGGCAGC TTTGGAGAGT
    CAACCTTTAC TAGGATTCAC AGTTACTCAA GTCAAAGATG AGAATTCAGA GTCTAGAGTG
    TTTCAGTTAC TGCACAAAAA CATGCTATTT TATGTATTCA AAGCAGATGA CGCTCACTCA
    GCTCAGAAGT GGATAGAAGC ATTTCAAGAA GGCACAATAC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005198277.1
    CDS153..4457
    Translation

    Target ORF information:

    RefSeq Version XM_005198220.3
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005198220.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA GTATCAGCAA ACACAGAAGA TTCCTCTATG
    AGTGGCTACT TGTACAGATC AAAGGGCAAT AAAAAACCTT GGAAACACTT GTGGTTTGTC
    ATAAAAAATA AAGTACTGTA TACATATGCT GCAAGTGAGG ATGTGGCAGC TTTGGAGAGT
    CAACCTTTAC TAGGATTCAC AGTTACTCAA GTCAAAGATG AGAATTCAGA GTCTAGAGTG
    TTTCAGTTAC TGCACAAAAA CATGCTATTT TATGTATTCA AAGCAGATGA CGCTCACTCA
    GCTCAGAAGT GGATAGAAGC ATTTCAAGAA GGCACAATAC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04187
    Clone ID Related Accession (Same CDS sequence) XM_005206128.3 , XM_005198220.3 , XM_005206128.4
    Accession Version XM_005206128.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206128.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA GTATCAGCAA ACACAGAAGA TTCCTCTATG
    AGTGGCTACT TGTACAGATC AAAGGGCAAT AAAAAACCTT GGAAACACTT GTGGTTTGTC
    ATAAAAAATA AAGTACTGTA TACATATGCT GCAAGTGAGG ATGTGGCAGC TTTGGAGAGT
    CAACCTTTAC TAGGATTCAC AGTTACTCAA GTCAAAGATG AGAATTCAGA GTCTAGAGTG
    TTTCAGTTAC TGCACAAAAA CATGCTATTT TATGTATTCA AAGCAGATGA CGCTCACTCA
    GCTCAGAAGT GGATAGAAGC ATTTCAAGAA GGCACAATAC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206185.1
    CDS308..4612
    Translation

    Target ORF information:

    RefSeq Version XM_005206128.4
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206128.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGCAGTTC TGGAGCATCC
    CAGGAGGGAC AAAGTGATCT GGACCTTGGT CTTGGTGACC TTCCTTCAGA TGAAGAGGAA
    GTCATCAACA GTTCAGATGA AGATGATGTC AGCTCTGCTT CTAGTAAAGG GGAGCCTGAT
    CTGCTGGACG ATAAACAGGG TGAAGATACT GGAATGAAAT GTAAAGTCCA TCATATTGCC
    AAAGAGATCA TGAGCTCAGA AAAAGTGTTT GTGGATGTGT TAAAACTTTT GCATATTGAT
    TTCCGGGATG CAGTAGCCCA TGCTTCCAGG CAACTTGGGA AACCAGTGAT TGAGGACCGG
    ATCCTAAACC AGATCCTGTA CTACTTGCCT CAGCTGTATG AGCTCAATCG GGATCTCCTG
    AAGGAGCTGG AGGAAAGAAT GTCAAACTGG ACGGAACAAC AAAGAATTGC TGATATCTTT
    GTAAAGAAGG GACCATATCT AAAAATGTAT TCCACATACA TCAAAGAATT TGATAAGAAT
    ATAGCTTTGT TGGATGAGCA GTGCAAGAAA AACCCAGGTT TTGCTGCTGT TGTTAGAGAA
    TTTGAGATGA GCCCACGCTG TGCTAATCTG GCCCTCAAGC ACTACCTGCT CAAGCCGGTT
    CAGAGGATTC CCCAGTACAG GCTGCTGCTA ACAGATTATT TGAAAAATCT CTTAGAAGAC
    TCTGGAGATT ACAGAGACAC TCAAGATGCC CTTGCTGTTG TTATAGAGGT AGCCAACCAC
    GCCAATGACA CCATGAAGCA AGGAGACAAC TTCCAGAAAC TTATGCAAAT TCAGTACAGC
    TTAAATGGAC ACCATGAAAT AGTTCAGCCT GGACGGGTTT TTCTCAAAGA AGGAATTCTG
    ATGAAGCTAT CTCGGAAAGT CATGCAGCCC CGAATGTTTT TCCTGTTTAA TGATGCCCTG
    TTGTATACAA CACCAGTGCA GTCTGGGATG TACAAACTGA ACAACATGCT ATCTTTGGCT
    GGAATGAAGG TCAGAAAACC CACCCAAGAA GCATATCAAA ATGAACTTAA GATCGAAAGT
    GTAGAACGTT CCTTCATCCT CTCAGCCAGT TCTGCCCCAG AAAGGGATGA ATGGCTCGAA
    GCCATTTCCA GGTCAATAGA AGAGTATGCC AAGAAAAGAA TCACATTCTG TCCTAGTCGG
    AGTCTTGATG AGGCTGACTC AGAAGATAAA GACGAGGTTA GTCCTCTTGG ATCTAAGGCT
    CCCATCTGGA TTCCTGATAC CAGGGCCACC ATGTGTATGA TCTGCACCAG TGAATTTACT
    CTCACCTGGA GACGACATCA CTGCCGGGCC TGCGGAAAGA TTGTATGCCA AGCTTGTTCA
    TCAAATAAGT ATGGCTTAGA TTACCTGAAA AATCAACCAG CAAGAGTATG TGAACACTGT
    TTCCAAGAAC TGCAGAAATT AGATCACCAG CACTCTCCTA AGTTTGGATC CCCTGGAAAT
    CACAAATCTC CTTCCAGCGC CTTATCATCA GTCTTACATA GTATTCCATC AGGGAGGAAA
    CAGAAAAAAA TCCCAGCTGC TCTGAAAGAA GTATCAGCAA ACACAGAAGA TTCCTCTATG
    AGTGGCTACT TGTACAGATC AAAGGGCAAT AAAAAACCTT GGAAACACTT GTGGTTTGTC
    ATAAAAAATA AAGTACTGTA TACATATGCT GCAAGTGAGG ATGTGGCAGC TTTGGAGAGT
    CAACCTTTAC TAGGATTCAC AGTTACTCAA GTCAAAGATG AGAATTCAGA GTCTAGAGTG
    TTTCAGTTAC TGCACAAAAA CATGCTATTT TATGTATTCA AAGCAGATGA CGCTCACTCA
    GCTCAGAAGT GGATAGAAGC ATTTCAAGAA GGCACAATAC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04188
    Clone ID Related Accession (Same CDS sequence) XM_002687205.5 , XM_002687205.6 , XM_587549.9
    Accession Version XM_002687205.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103917.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002687205.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGTTCTGG AGCATCCCAG
    GAGGGACAAA GTGATCTGGA CCTTGGTCTT GGTGACCTTC CTTCAGATGA AGAGGAAGTC
    ATCAACAGTT CAGATGAAGA TGATGTCAGC TCTGCTTCTA GTAAAGGGGA GCCTGATCTG
    CTGGACGATA AACAGGGTGA AGATACTGGA ATGAAATGTA AAGTCCATCA TATTGCCAAA
    GAGATCATGA GCTCAGAAAA AGTGTTTGTG GATGTGTTAA AACTTTTGCA TATTGATTTC
    CGGGATGCAG TAGCCCATGC TTCCAGGCAA CTTGGGAAAC CAGTGATTGA GGACCGGATC
    CTAAACCAGA TCCTGTACTA CTTGCCTCAG CTGTATGAGC TCAATCGGGA TCTCCTGAAG
    GAGCTGGAGG AAAGAATGTC AAACTGGACG GAACAACAAA GAATTGCTGA TATCTTTGTA
    AAGAAGGGAC CATATCTAAA AATGTATTCC ACATACATCA AAGAATTTGA TAAGAATATA
    GCTTTGTTGG ATGAGCAGTG CAAGAAAAAC CCAGGTTTTG CTGCTGTTGT TAGAGAATTT
    GAGATGAGCC CACGCTGTGC TAATCTGGCC CTCAAGCACT ACCTGCTCAA GCCGGTTCAG
    AGGATTCCCC AGTACAGGCT GCTGCTAACA GATTATTTGA AAAATCTCTT AGAAGACTCT
    GGAGATTACA GAGACACTCA AGATGCCCTT GCTGTTGTTA TAGAGGTAGC CAACCACGCC
    AATGACACCA TGAAGCAAGG AGACAACTTC CAGAAACTTA TGCAAATTCA GTACAGCTTA
    AATGGACACC ATGAAATAGT TCAGCCTGGA CGGGTTTTTC TCAAAGAAGG AATTCTGATG
    AAGCTATCTC GGAAAGTCAT GCAGCCCCGA ATGTTTTTCC TGTTTAATGA TGCCCTGTTG
    TATACAACAC CAGTGCAGTC TGGGATGTAC AAACTGAACA ACATGCTATC TTTGGCTGGA
    ATGAAGGTCA GAAAACCCAC CCAAGAAGCA TATCAAAATG AACTTAAGAT CGAAAGTGTA
    GAACGTTCCT TCATCCTCTC AGCCAGTTCT GCCCCAGAAA GGGATGAATG GCTCGAAGCC
    ATTTCCAGGT CAATAGAAGA GTATGCCAAG AAAAGAATCA CATTCTGTCC TAGTCGGAGT
    CTTGATGAGG CTGACTCAGA AGATAAAGAC GAGGTTAGTC CTCTTGGATC TAAGGCTCCC
    ATCTGGATTC CTGATACCAG GGCCACCATG TGTATGATCT GCACCAGTGA ATTTACTCTC
    ACCTGGAGAC GACATCACTG CCGGGCCTGC GGAAAGATTG TATGCCAAGC TTGTTCATCA
    AATAAGTATG GCTTAGATTA CCTGAAAAAT CAACCAGCAA GAGTATGTGA ACACTGTTTC
    CAAGAACTGC AGAAATTAGA TCACCAGCAC TCTCCTAAGT TTGGATCCCC TGGAAATCAC
    AAATCTCCTT CCAGCGCCTT ATCATCAGTC TTACATAGTA TTCCATCAGG GAGGAAACAG
    AAAAAAATCC CAGCTGCTCT GAAAGAAGTA TCAGCAAACA CAGAAGATTC CTCTATGAGT
    GGCTACTTGT ACAGATCAAA GGGCAATAAA AAACCTTGGA AACACTTGTG GTTTGTCATA
    AAAAATAAAG TACTGTATAC ATATGCTGCA AGTGAGGATG TGGCAGCTTT GGAGAGTCAA
    CCTTTACTAG GATTCACAGT TACTCAAGTC AAAGATGAGA ATTCAGAGTC TAGAGTGTTT
    CAGTTACTGC ACAAAAACAT GCTATTTTAT GTATTCAAAG CAGATGACGC TCACTCAGCT
    CAGAAGTGGA TAGAAGCATT TCAAGAAGGC ACAATACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002687251.1
    CDS153..4454
    Translation

    Target ORF information:

    RefSeq Version XM_002687205.5
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002687205.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGTTCTGG AGCATCCCAG
    GAGGGACAAA GTGATCTGGA CCTTGGTCTT GGTGACCTTC CTTCAGATGA AGAGGAAGTC
    ATCAACAGTT CAGATGAAGA TGATGTCAGC TCTGCTTCTA GTAAAGGGGA GCCTGATCTG
    CTGGACGATA AACAGGGTGA AGATACTGGA ATGAAATGTA AAGTCCATCA TATTGCCAAA
    GAGATCATGA GCTCAGAAAA AGTGTTTGTG GATGTGTTAA AACTTTTGCA TATTGATTTC
    CGGGATGCAG TAGCCCATGC TTCCAGGCAA CTTGGGAAAC CAGTGATTGA GGACCGGATC
    CTAAACCAGA TCCTGTACTA CTTGCCTCAG CTGTATGAGC TCAATCGGGA TCTCCTGAAG
    GAGCTGGAGG AAAGAATGTC AAACTGGACG GAACAACAAA GAATTGCTGA TATCTTTGTA
    AAGAAGGGAC CATATCTAAA AATGTATTCC ACATACATCA AAGAATTTGA TAAGAATATA
    GCTTTGTTGG ATGAGCAGTG CAAGAAAAAC CCAGGTTTTG CTGCTGTTGT TAGAGAATTT
    GAGATGAGCC CACGCTGTGC TAATCTGGCC CTCAAGCACT ACCTGCTCAA GCCGGTTCAG
    AGGATTCCCC AGTACAGGCT GCTGCTAACA GATTATTTGA AAAATCTCTT AGAAGACTCT
    GGAGATTACA GAGACACTCA AGATGCCCTT GCTGTTGTTA TAGAGGTAGC CAACCACGCC
    AATGACACCA TGAAGCAAGG AGACAACTTC CAGAAACTTA TGCAAATTCA GTACAGCTTA
    AATGGACACC ATGAAATAGT TCAGCCTGGA CGGGTTTTTC TCAAAGAAGG AATTCTGATG
    AAGCTATCTC GGAAAGTCAT GCAGCCCCGA ATGTTTTTCC TGTTTAATGA TGCCCTGTTG
    TATACAACAC CAGTGCAGTC TGGGATGTAC AAACTGAACA ACATGCTATC TTTGGCTGGA
    ATGAAGGTCA GAAAACCCAC CCAAGAAGCA TATCAAAATG AACTTAAGAT CGAAAGTGTA
    GAACGTTCCT TCATCCTCTC AGCCAGTTCT GCCCCAGAAA GGGATGAATG GCTCGAAGCC
    ATTTCCAGGT CAATAGAAGA GTATGCCAAG AAAAGAATCA CATTCTGTCC TAGTCGGAGT
    CTTGATGAGG CTGACTCAGA AGATAAAGAC GAGGTTAGTC CTCTTGGATC TAAGGCTCCC
    ATCTGGATTC CTGATACCAG GGCCACCATG TGTATGATCT GCACCAGTGA ATTTACTCTC
    ACCTGGAGAC GACATCACTG CCGGGCCTGC GGAAAGATTG TATGCCAAGC TTGTTCATCA
    AATAAGTATG GCTTAGATTA CCTGAAAAAT CAACCAGCAA GAGTATGTGA ACACTGTTTC
    CAAGAACTGC AGAAATTAGA TCACCAGCAC TCTCCTAAGT TTGGATCCCC TGGAAATCAC
    AAATCTCCTT CCAGCGCCTT ATCATCAGTC TTACATAGTA TTCCATCAGG GAGGAAACAG
    AAAAAAATCC CAGCTGCTCT GAAAGAAGTA TCAGCAAACA CAGAAGATTC CTCTATGAGT
    GGCTACTTGT ACAGATCAAA GGGCAATAAA AAACCTTGGA AACACTTGTG GTTTGTCATA
    AAAAATAAAG TACTGTATAC ATATGCTGCA AGTGAGGATG TGGCAGCTTT GGAGAGTCAA
    CCTTTACTAG GATTCACAGT TACTCAAGTC AAAGATGAGA ATTCAGAGTC TAGAGTGTTT
    CAGTTACTGC ACAAAAACAT GCTATTTTAT GTATTCAAAG CAGATGACGC TCACTCAGCT
    CAGAAGTGGA TAGAAGCATT TCAAGAAGGC ACAATACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04188
    Clone ID Related Accession (Same CDS sequence) XM_002687205.5 , XM_002687205.6 , XM_587549.9
    Accession Version XM_002687205.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002687205.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGTTCTGG AGCATCCCAG
    GAGGGACAAA GTGATCTGGA CCTTGGTCTT GGTGACCTTC CTTCAGATGA AGAGGAAGTC
    ATCAACAGTT CAGATGAAGA TGATGTCAGC TCTGCTTCTA GTAAAGGGGA GCCTGATCTG
    CTGGACGATA AACAGGGTGA AGATACTGGA ATGAAATGTA AAGTCCATCA TATTGCCAAA
    GAGATCATGA GCTCAGAAAA AGTGTTTGTG GATGTGTTAA AACTTTTGCA TATTGATTTC
    CGGGATGCAG TAGCCCATGC TTCCAGGCAA CTTGGGAAAC CAGTGATTGA GGACCGGATC
    CTAAACCAGA TCCTGTACTA CTTGCCTCAG CTGTATGAGC TCAATCGGGA TCTCCTGAAG
    GAGCTGGAGG AAAGAATGTC AAACTGGACG GAACAACAAA GAATTGCTGA TATCTTTGTA
    AAGAAGGGAC CATATCTAAA AATGTATTCC ACATACATCA AAGAATTTGA TAAGAATATA
    GCTTTGTTGG ATGAGCAGTG CAAGAAAAAC CCAGGTTTTG CTGCTGTTGT TAGAGAATTT
    GAGATGAGCC CACGCTGTGC TAATCTGGCC CTCAAGCACT ACCTGCTCAA GCCGGTTCAG
    AGGATTCCCC AGTACAGGCT GCTGCTAACA GATTATTTGA AAAATCTCTT AGAAGACTCT
    GGAGATTACA GAGACACTCA AGATGCCCTT GCTGTTGTTA TAGAGGTAGC CAACCACGCC
    AATGACACCA TGAAGCAAGG AGACAACTTC CAGAAACTTA TGCAAATTCA GTACAGCTTA
    AATGGACACC ATGAAATAGT TCAGCCTGGA CGGGTTTTTC TCAAAGAAGG AATTCTGATG
    AAGCTATCTC GGAAAGTCAT GCAGCCCCGA ATGTTTTTCC TGTTTAATGA TGCCCTGTTG
    TATACAACAC CAGTGCAGTC TGGGATGTAC AAACTGAACA ACATGCTATC TTTGGCTGGA
    ATGAAGGTCA GAAAACCCAC CCAAGAAGCA TATCAAAATG AACTTAAGAT CGAAAGTGTA
    GAACGTTCCT TCATCCTCTC AGCCAGTTCT GCCCCAGAAA GGGATGAATG GCTCGAAGCC
    ATTTCCAGGT CAATAGAAGA GTATGCCAAG AAAAGAATCA CATTCTGTCC TAGTCGGAGT
    CTTGATGAGG CTGACTCAGA AGATAAAGAC GAGGTTAGTC CTCTTGGATC TAAGGCTCCC
    ATCTGGATTC CTGATACCAG GGCCACCATG TGTATGATCT GCACCAGTGA ATTTACTCTC
    ACCTGGAGAC GACATCACTG CCGGGCCTGC GGAAAGATTG TATGCCAAGC TTGTTCATCA
    AATAAGTATG GCTTAGATTA CCTGAAAAAT CAACCAGCAA GAGTATGTGA ACACTGTTTC
    CAAGAACTGC AGAAATTAGA TCACCAGCAC TCTCCTAAGT TTGGATCCCC TGGAAATCAC
    AAATCTCCTT CCAGCGCCTT ATCATCAGTC TTACATAGTA TTCCATCAGG GAGGAAACAG
    AAAAAAATCC CAGCTGCTCT GAAAGAAGTA TCAGCAAACA CAGAAGATTC CTCTATGAGT
    GGCTACTTGT ACAGATCAAA GGGCAATAAA AAACCTTGGA AACACTTGTG GTTTGTCATA
    AAAAATAAAG TACTGTATAC ATATGCTGCA AGTGAGGATG TGGCAGCTTT GGAGAGTCAA
    CCTTTACTAG GATTCACAGT TACTCAAGTC AAAGATGAGA ATTCAGAGTC TAGAGTGTTT
    CAGTTACTGC ACAAAAACAT GCTATTTTAT GTATTCAAAG CAGATGACGC TCACTCAGCT
    CAGAAGTGGA TAGAAGCATT TCAAGAAGGC ACAATACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002687251.1
    CDS305..4606
    Translation

    Target ORF information:

    RefSeq Version XM_002687205.6
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002687205.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGTTCTGG AGCATCCCAG
    GAGGGACAAA GTGATCTGGA CCTTGGTCTT GGTGACCTTC CTTCAGATGA AGAGGAAGTC
    ATCAACAGTT CAGATGAAGA TGATGTCAGC TCTGCTTCTA GTAAAGGGGA GCCTGATCTG
    CTGGACGATA AACAGGGTGA AGATACTGGA ATGAAATGTA AAGTCCATCA TATTGCCAAA
    GAGATCATGA GCTCAGAAAA AGTGTTTGTG GATGTGTTAA AACTTTTGCA TATTGATTTC
    CGGGATGCAG TAGCCCATGC TTCCAGGCAA CTTGGGAAAC CAGTGATTGA GGACCGGATC
    CTAAACCAGA TCCTGTACTA CTTGCCTCAG CTGTATGAGC TCAATCGGGA TCTCCTGAAG
    GAGCTGGAGG AAAGAATGTC AAACTGGACG GAACAACAAA GAATTGCTGA TATCTTTGTA
    AAGAAGGGAC CATATCTAAA AATGTATTCC ACATACATCA AAGAATTTGA TAAGAATATA
    GCTTTGTTGG ATGAGCAGTG CAAGAAAAAC CCAGGTTTTG CTGCTGTTGT TAGAGAATTT
    GAGATGAGCC CACGCTGTGC TAATCTGGCC CTCAAGCACT ACCTGCTCAA GCCGGTTCAG
    AGGATTCCCC AGTACAGGCT GCTGCTAACA GATTATTTGA AAAATCTCTT AGAAGACTCT
    GGAGATTACA GAGACACTCA AGATGCCCTT GCTGTTGTTA TAGAGGTAGC CAACCACGCC
    AATGACACCA TGAAGCAAGG AGACAACTTC CAGAAACTTA TGCAAATTCA GTACAGCTTA
    AATGGACACC ATGAAATAGT TCAGCCTGGA CGGGTTTTTC TCAAAGAAGG AATTCTGATG
    AAGCTATCTC GGAAAGTCAT GCAGCCCCGA ATGTTTTTCC TGTTTAATGA TGCCCTGTTG
    TATACAACAC CAGTGCAGTC TGGGATGTAC AAACTGAACA ACATGCTATC TTTGGCTGGA
    ATGAAGGTCA GAAAACCCAC CCAAGAAGCA TATCAAAATG AACTTAAGAT CGAAAGTGTA
    GAACGTTCCT TCATCCTCTC AGCCAGTTCT GCCCCAGAAA GGGATGAATG GCTCGAAGCC
    ATTTCCAGGT CAATAGAAGA GTATGCCAAG AAAAGAATCA CATTCTGTCC TAGTCGGAGT
    CTTGATGAGG CTGACTCAGA AGATAAAGAC GAGGTTAGTC CTCTTGGATC TAAGGCTCCC
    ATCTGGATTC CTGATACCAG GGCCACCATG TGTATGATCT GCACCAGTGA ATTTACTCTC
    ACCTGGAGAC GACATCACTG CCGGGCCTGC GGAAAGATTG TATGCCAAGC TTGTTCATCA
    AATAAGTATG GCTTAGATTA CCTGAAAAAT CAACCAGCAA GAGTATGTGA ACACTGTTTC
    CAAGAACTGC AGAAATTAGA TCACCAGCAC TCTCCTAAGT TTGGATCCCC TGGAAATCAC
    AAATCTCCTT CCAGCGCCTT ATCATCAGTC TTACATAGTA TTCCATCAGG GAGGAAACAG
    AAAAAAATCC CAGCTGCTCT GAAAGAAGTA TCAGCAAACA CAGAAGATTC CTCTATGAGT
    GGCTACTTGT ACAGATCAAA GGGCAATAAA AAACCTTGGA AACACTTGTG GTTTGTCATA
    AAAAATAAAG TACTGTATAC ATATGCTGCA AGTGAGGATG TGGCAGCTTT GGAGAGTCAA
    CCTTTACTAG GATTCACAGT TACTCAAGTC AAAGATGAGA ATTCAGAGTC TAGAGTGTTT
    CAGTTACTGC ACAAAAACAT GCTATTTTAT GTATTCAAAG CAGATGACGC TCACTCAGCT
    CAGAAGTGGA TAGAAGCATT TCAAGAAGGC ACAATACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa04188
    Clone ID Related Accession (Same CDS sequence) XM_002687205.5 , XM_002687205.6 , XM_587549.9
    Accession Version XM_587549.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4302bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product FYVE, RhoGEF and PH domain-containing protein 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644784.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_587549.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGTTCTGG AGCATCCCAG
    GAGGGACAAA GTGATCTGGA CCTTGGTCTT GGTGACCTTC CTTCAGATGA AGAGGAAGTC
    ATCAACAGTT CAGATGAAGA TGATGTCAGC TCTGCTTCTA GTAAAGGGGA GCCTGATCTG
    CTGGACGATA AACAGGGTGA AGATACTGGA ATGAAATGTA AAGTCCATCA TATTGCCAAA
    GAGATCATGA GCTCAGAAAA AGTGTTTGTG GATGTGTTAA AACTTTTGCA TATTGATTTC
    CGGGATGCAG TAGCCCATGC TTCCAGGCAA CTTGGGAAAC CAGTGATTGA GGACCGGATC
    CTAAACCAGA TCCTGTACTA CTTGCCTCAG CTGTATGAGC TCAATCGGGA TCTCCTGAAG
    GAGCTGGAGG AAAGAATGTC AAACTGGACG GAACAACAAA GAATTGCTGA TATCTTTGTA
    AAGAAGGGAC CATATCTAAA AATGTATTCC ACATACATCA AAGAATTTGA TAAGAATATA
    GCTTTGTTGG ATGAGCAGTG CAAGAAAAAC CCAGGTTTTG CTGCTGTTGT TAGAGAATTT
    GAGATGAGCC CACGCTGTGC TAATCTGGCC CTCAAGCACT ACCTGCTCAA GCCGGTTCAG
    AGGATTCCCC AGTACAGGCT GCTGCTAACA GATTATTTGA AAAATCTCTT AGAAGACTCT
    GGAGATTACA GAGACACTCA AGATGCCCTT GCTGTTGTTA TAGAGGTAGC CAACCACGCC
    AATGACACCA TGAAGCAAGG AGACAACTTC CAGAAACTTA TGCAAATTCA GTACAGCTTA
    AATGGACACC ATGAAATAGT TCAGCCTGGA CGGGTTTTTC TCAAAGAAGG AATTCTGATG
    AAGCTATCTC GGAAAGTCAT GCAGCCCCGA ATGTTTTTCC TGTTTAATGA TGCCCTGTTG
    TATACAACAC CAGTGCAGTC TGGGATGTAC AAACTGAACA ACATGCTATC TTTGGCTGGA
    ATGAAGGTCA GAAAACCCAC CCAAGAAGCA TATCAAAATG AACTTAAGAT CGAAAGTGTA
    GAACGTTCCT TCATCCTCTC AGCCAGTTCT GCCCCAGAAA GGGATGAATG GCTCGAAGCC
    ATTTCCAGGT CAATAGAAGA GTATGCCAAG AAAAGAATCA CATTCTGTCC TAGTCGGAGT
    CTTGATGAGG CTGACTCAGA AGATAAAGAC GAGGTTAGTC CTCTTGGATC TAAGGCTCCC
    ATCTGGATTC CTGATACCAG GGCCACCATG TGTATGATCT GCACCAGTGA ATTTACTCTC
    ACCTGGAGAC GACATCACTG CCGGGCCTGC GGAAAGATTG TATGCCAAGC TTGTTCATCA
    AATAAGTATG GCTTAGATTA CCTGAAAAAT CAACCAGCAA GAGTATGTGA ACACTGTTTC
    CAAGAACTGC AGAAATTAGA TCACCAGCAC TCTCCTAAGT TTGGATCCCC TGGAAATCAC
    AAATCTCCTT CCAGCGCCTT ATCATCAGTC TTACATAGTA TTCCATCAGG GAGGAAACAG
    AAAAAAATCC CAGCTGCTCT GAAAGAAGTA TCAGCAAACA CAGAAGATTC CTCTATGAGT
    GGCTACTTGT ACAGATCAAA GGGCAATAAA AAACCTTGGA AACACTTGTG GTTTGTCATA
    AAAAATAAAG TACTGTATAC ATATGCTGCA AGTGAGGATG TGGCAGCTTT GGAGAGTCAA
    CCTTTACTAG GATTCACAGT TACTCAAGTC AAAGATGAGA ATTCAGAGTC TAGAGTGTTT
    CAGTTACTGC ACAAAAACAT GCTATTTTAT GTATTCAAAG CAGATGACGC TCACTCAGCT
    CAGAAGTGGA TAGAAGCATT TCAAGAAGGC ACAATACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_587549.4
    CDS153..4454
    Translation

    Target ORF information:

    RefSeq Version XM_587549.9
    Organism Bos taurus(cattle)
    Definition Bos taurus FYVE, RhoGEF and PH domain containing 6 (FGD6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_587549.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACTTCTG CTGCAGAGAT TAAGAAGCCA CCAGTGGCCC CCAAACCAAA GTTTGTTCTG 
    GCAGCTAATA AGCCAGCTCC ACCTCCTGTC GCACCTAAAC CTGAGATCTT GGTTTCTAGT
    GTTCCACAGT CAACAAAGAA AACCAAACCA TCCATAGCTC CAAAACCAAA AGTTCTGAAG
    GCCTCACCTG TCCAGGACAT CACACAGTCA CCATCAAGGA AAATCGTCGT GAACCTGGAA
    GAGCATACAC AGGAATTCCC TGAAAGTATT GACAACTCGA ACTGCAGAAA CAGAGGGCAT
    CCGAGCAGTG ATTATATTTT ACCAGTGTGT TCCTGCCATC CTGGATGTAA CCATAAGCTT
    GAGAGCAGAA AGAATTTGTA TGTCAAGCAG CTTGTCTTAG GGCCCCTGGA AATGCATGAG
    AATTTAGAAA ACAACAAAAT TGGTGAGTCC CCTTCAGCTG TGAAAAATCA GCATAAATGT
    GATTCTGTTG GTGAAAGGGT CAAGAGCCAG AGTAAAGTAG TTTTAAAGGC AAGCATCCTC
    GAAGAGAAGC TCAAAGATGT TTTAACACAA CCAATGTCAC CCTTTCTTTT CCCCCAGAAG
    CACAGATCCA CAGACAGTCC AGAAATGAGT GATGGCTGTA ATTCCCACAG ACAATTCAGC
    ATTGAATTTG CAGATTTGTC ACCTTCCCTG TCCAGCTTTG AAAACGTTTC TAATCATCAC
    CCTTGCCACC TACAACTTCC TAGCACTGAA TTTCAAAATT TTGAAACTAA TCAGAATGAC
    AGTGAAAAAA GCAACACTTG CTATCATTCA TCTGAACAAG AAGCTCTAGA AAATGGAAAA
    AGGAGTCCTT TCTTATCTTC AGATGGAGAT AGTAAGAAAT CAGAAGCCAG AGACCTTGGT
    CCCTTGGAAA TCCACTTAGT GCCATGTACT CCCAAATTTC CAACTCCCAA GCCCAGAAAA
    ACACATGTTG CACGCCTCTT ACGCCAGAGA CATATAGATA CTTCTAGTGA AAGTACTGAA
    GAACCAGAGG ATGTAGACAG CTTGCCTCAT GTTTTCGAAG ATAGTTTGAA AAACAATAAA
    ATCAGTGTCT TTCATCAGAC TGCTTTGTGT AACCAGGAAC AGGTGGACAA AGTGAAGCCA
    GAAAATAAAG GTGAATTGAA TGTGGACCTC ACTGGTGACA GACAAAACTC GGTCTATTCA
    CAGAAAGCCT TGCGTCCTGA AACATTTTCC TCTGAAAAAA CGGCACCTCC TTTAGAAACA
    GACTCTAACT TGAGTTCTGA CGACAAGTCA GGAGATAGTT CTGGTATGTC ACTTGCCGAG
    GACAAAGGAA TCAGTTTTAT AAGATGCAGT ACTCTATCTA TGAGCCTGCC CAAGCAGCTC
    AAATTAACCT GCAGCGAACA TTTGCCTACT GCCTCTAACC CAGGAGTGTC TGCCCCTCAA
    ATGCAAAAGG AATCTATGAT AAAAGGTGAA AGTTCCTCAA AAGTTGTCCC CAAAAAACCT
    CAAAGACACA GTTTGCCTGC TGCCGGAGTG CTTAAAAAGG CTGCCTCTGA GGAGCTTTTG
    GAGAAAAGTT CTTATCCCTC AAATGAAGAA AAAGATGCAG AGAAGGGTCT AGAAAGAGAT
    CACGTGCAGC ATTTATGTGC CCCAAACAAT GGTATGTCAT CCTCCTTTGA TATGCCTAAA
    CGGGCTTCAG AGAAGCCAGT GTGGAAATTA CCTCATCCCA TTATCCCCTT TTCAGGGAAC
    CCAGAATCCT TAAAGTCTGT CACCATATCC TCAAACAGTG AGACTCCCAC ACCCCTGACC
    AAGCCTAGAG CAAAATCATT ATCTGCTATG GATGTGGAAA GGTGCACTAA GCCATGCAAA
    GACTCTCACA AGAAGAACTC TTTAAAGAAG TTGCTCAATA TGAAGCTGTC CATCTGTTTC
    ATGAAGAGTG ACTTCCAGAA ATTTTGGTCC AAGCATAGCC ATCTTGGAGA CACAACCTCA
    GGCAGCCTGG CAGGTGGGGA GCAAAGAGGG AGTGAGAGTG ATTGGCATGG CCTGTTGGTA
    GGAGAAGAGA AGAGAAGTAA ACCCATCAAG GCATATTCGG CAGATAACTA TAGCCTGGAG
    TCCCACAAGA AGAGGAAGAA GTTGCGGGGC CACACCAATG CAGCTAATGG ACCAAGAGCC
    GAGTCTCTGG ATGACCAAAT GCTGTCCAGG GAGTCGTCCT TGCAGGCACC TTGCAAATCT
    GTTACCAGCG ACTGTGTACC AGAGTATGAA AATATACGGC ATTATGAGGA AATACCAGAG
    TATGAGAACT TGCCCTTTGT TATGGCTATG GGGAAAACTC CCGAGTTGGA ACGACGGAAC
    TCCAGCAGCG TGGAGGACAC TGATGCAAAT GTGTATGAGG TACAGGAGCC GTATGAAGCT
    CCAGACGGCC AGCTCCAACT CGGACCCAGA CACCAGCATT CCAGTTCTGG AGCATCCCAG
    GAGGGACAAA GTGATCTGGA CCTTGGTCTT GGTGACCTTC CTTCAGATGA AGAGGAAGTC
    ATCAACAGTT CAGATGAAGA TGATGTCAGC TCTGCTTCTA GTAAAGGGGA GCCTGATCTG
    CTGGACGATA AACAGGGTGA AGATACTGGA ATGAAATGTA AAGTCCATCA TATTGCCAAA
    GAGATCATGA GCTCAGAAAA AGTGTTTGTG GATGTGTTAA AACTTTTGCA TATTGATTTC
    CGGGATGCAG TAGCCCATGC TTCCAGGCAA CTTGGGAAAC CAGTGATTGA GGACCGGATC
    CTAAACCAGA TCCTGTACTA CTTGCCTCAG CTGTATGAGC TCAATCGGGA TCTCCTGAAG
    GAGCTGGAGG AAAGAATGTC AAACTGGACG GAACAACAAA GAATTGCTGA TATCTTTGTA
    AAGAAGGGAC CATATCTAAA AATGTATTCC ACATACATCA AAGAATTTGA TAAGAATATA
    GCTTTGTTGG ATGAGCAGTG CAAGAAAAAC CCAGGTTTTG CTGCTGTTGT TAGAGAATTT
    GAGATGAGCC CACGCTGTGC TAATCTGGCC CTCAAGCACT ACCTGCTCAA GCCGGTTCAG
    AGGATTCCCC AGTACAGGCT GCTGCTAACA GATTATTTGA AAAATCTCTT AGAAGACTCT
    GGAGATTACA GAGACACTCA AGATGCCCTT GCTGTTGTTA TAGAGGTAGC CAACCACGCC
    AATGACACCA TGAAGCAAGG AGACAACTTC CAGAAACTTA TGCAAATTCA GTACAGCTTA
    AATGGACACC ATGAAATAGT TCAGCCTGGA CGGGTTTTTC TCAAAGAAGG AATTCTGATG
    AAGCTATCTC GGAAAGTCAT GCAGCCCCGA ATGTTTTTCC TGTTTAATGA TGCCCTGTTG
    TATACAACAC CAGTGCAGTC TGGGATGTAC AAACTGAACA ACATGCTATC TTTGGCTGGA
    ATGAAGGTCA GAAAACCCAC CCAAGAAGCA TATCAAAATG AACTTAAGAT CGAAAGTGTA
    GAACGTTCCT TCATCCTCTC AGCCAGTTCT GCCCCAGAAA GGGATGAATG GCTCGAAGCC
    ATTTCCAGGT CAATAGAAGA GTATGCCAAG AAAAGAATCA CATTCTGTCC TAGTCGGAGT
    CTTGATGAGG CTGACTCAGA AGATAAAGAC GAGGTTAGTC CTCTTGGATC TAAGGCTCCC
    ATCTGGATTC CTGATACCAG GGCCACCATG TGTATGATCT GCACCAGTGA ATTTACTCTC
    ACCTGGAGAC GACATCACTG CCGGGCCTGC GGAAAGATTG TATGCCAAGC TTGTTCATCA
    AATAAGTATG GCTTAGATTA CCTGAAAAAT CAACCAGCAA GAGTATGTGA ACACTGTTTC
    CAAGAACTGC AGAAATTAGA TCACCAGCAC TCTCCTAAGT TTGGATCCCC TGGAAATCAC
    AAATCTCCTT CCAGCGCCTT ATCATCAGTC TTACATAGTA TTCCATCAGG GAGGAAACAG
    AAAAAAATCC CAGCTGCTCT GAAAGAAGTA TCAGCAAACA CAGAAGATTC CTCTATGAGT
    GGCTACTTGT ACAGATCAAA GGGCAATAAA AAACCTTGGA AACACTTGTG GTTTGTCATA
    AAAAATAAAG TACTGTATAC ATATGCTGCA AGTGAGGATG TGGCAGCTTT GGAGAGTCAA
    CCTTTACTAG GATTCACAGT TACTCAAGTC AAAGATGAGA ATTCAGAGTC TAGAGTGTTT
    CAGTTACTGC ACAAAAACAT GCTATTTTAT GTATTCAAAG CAGATGACGC TCACTCAGCT
    CAGAAGTGGA TAGAAGCATT TCAAGAAGGC ACAATACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.