MPZ cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MPZ
Entrez Gene ID	539462
Full Name	myelin protein zero
General protein information	Preferred Names myelin protein zero Names myelin protein P0 MPP myelin peripheral protein myelin protein zero (Charcot-Marie-Tooth neuropathy 1B)
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in the orthologous gene in human are associated with myelinating neuropathies. A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316359.1	NP_001303288.1	myelin protein P0 isoform L-MPZ precursor
NM_001079507.2	NP_001072975.1	myelin protein P0 isoform MPZ precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100859605	XP_003643863.1
Danio rerio (zebrafish)	mpz	NP_919342.1
Canis lupus familiaris (dog)	MPZ	XP_545771.1
Pan troglodytes (chimpanzee)	MPZ	XP_001173975.1
Mus musculus (house mouse)	Mpz	NP_032649.2
Homo sapiens (human)	MPZ	NP_000521.2
	LOC719969	XP_001118030.1
Xenopus tropicalis (tropical clawed frog)	mpz	NP_001072741.1
Bos taurus (cattle)	MPZ	NP_001072975.1
Rattus norvegicus (Norway rat)	Mpz	NP_058723.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon are Essential for Translational Readthrough to Produce L-MPZ.
Yamaguchi Y, Baba H
Neurochemical research43(1)227-237(2018 Jan)

L-MPZ, a novel isoform of myelin P0, is produced by stop codon readthrough.
Yamaguchi Y, Hayashi A, Campagnoni CW, Kimura A, Inuzuka T, Baba H
The Journal of biological chemistry287(21)17765-76(2012 May)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Beier H, Grimm M
Nucleic acids research29(23)4767-82(2001 Dec)

Complete amino acid sequence of PO protein in bovine peripheral nerve myelin.
Sakamoto Y, Kitamura K, Yoshimura K, Nishijima T, Uyemura K
The Journal of biological chemistry262(9)4208-14(1987 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MPZ gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MPZ cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43006	NM_001079507.2 Latest version!	Bos taurus myelin protein zero (MPZ), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa95422	NM_001316359.1 Latest version!	Bos taurus myelin protein zero (MPZ), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MPZ ( NM_001079507.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001072975.1 Bos taurus myelin protein zero (MPZ), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa43006
Clone ID Related Accession (Same CDS sequence)	NM_001079507.2
Accession Version	NM_001079507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Bos taurus(cattle)
Product	myelin protein P0 isoform MPZ precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EE369960.1 and BC122659.1. On Oct 16, 2015 this sequence version replaced NM_001079507.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MPZ, also known as P0) results from translation termination at the upstream UAG stop codon, while the longer isoform (L-MPZ) results from UAG stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MPZ). ##Evidence-Data-START## Transcript exon combination :: BC122659.1, DV917420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTG GGGCTCCCTC GTCCAGCCCC AGTCCTATCC TGGCTGCGCT ACTCTTCTCC 
TCCTTGGTGC TCTCCCCTGT CCAGGCCATT GTGGTCTACA CAGACAAGGA GGTCCACGGT 
GCTGTGGGCT CCCAGGTGAC CCTGTACTGC TCCTTCTGGT CCAGTGAGTG GGTTTCAGAT 
GACCTCTCCT TCACCTGGCG CTACCAGCCC GAAGGGGGCC GTGATGCCAT CTCGATCTTC 
CACTACGCCA AGGGACAGCC CTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCACCG GAAGGATGGC TCCATTGTCA TACACAACCT GGACTATGGT 
GACAACGGCA CTTTCACCTG TGACGTCAAA AACCCCCCAG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCTACGAGGT ACGGGGTGGT GCTGGGAGCC 
GTGATCGGGG GCGTCCTGGG GGTGGTGCTG TTGGCGCTGC TGCTTTTCTA CCTGATTCGG 
TACTGCTGGC TACGCAGGCA GGCGGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGC 
AAATTGCACA AGACTGCGAA GGACGCCTCA AAGCGCGGCC GGCAGACGCC GGTTCTGTAT 
GCCATGCTGG ACCACAGCAG AAGCACCAAA GCTGCCAGTG AGAAGAAGAC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072975.1
CDS	51..797
Translation	MAPGAPSSSPSPILAALLFSSLVLSPVQAIVVYTDKEVHGAVGSQVTLYCSFWSSEWVSDDLSFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPHRKDGSIVIHNLDYGDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGAVIGGVLGVVLLALLLFYLIRYCWLRRQAALQRRLSAMEKGKLHKTAKDASKRGRQTPVLYAMLDHSRSTKAASEKKTKGLGESRKDKK

Target ORF information:

RefSeq Version	NM_001079507.2
Organism	Bos taurus(cattle)
Definition	Bos taurus myelin protein zero (MPZ), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079507.2

ORF Insert Sequence:

ATGGCTCCTG GGGCTCCCTC GTCCAGCCCC AGTCCTATCC TGGCTGCGCT ACTCTTCTCC 
TCCTTGGTGC TCTCCCCTGT CCAGGCCATT GTGGTCTACA CAGACAAGGA GGTCCACGGT 
GCTGTGGGCT CCCAGGTGAC CCTGTACTGC TCCTTCTGGT CCAGTGAGTG GGTTTCAGAT 
GACCTCTCCT TCACCTGGCG CTACCAGCCC GAAGGGGGCC GTGATGCCAT CTCGATCTTC 
CACTACGCCA AGGGACAGCC CTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCACCG GAAGGATGGC TCCATTGTCA TACACAACCT GGACTATGGT 
GACAACGGCA CTTTCACCTG TGACGTCAAA AACCCCCCAG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCTACGAGGT ACGGGGTGGT GCTGGGAGCC 
GTGATCGGGG GCGTCCTGGG GGTGGTGCTG TTGGCGCTGC TGCTTTTCTA CCTGATTCGG 
TACTGCTGGC TACGCAGGCA GGCGGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGC 
AAATTGCACA AGACTGCGAA GGACGCCTCA AAGCGCGGCC GGCAGACGCC GGTTCTGTAT 
GCCATGCTGG ACCACAGCAG AAGCACCAAA GCTGCCAGTG AGAAGAAGAC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MPZ ( NM_001316359.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001303288.1 Bos taurus myelin protein zero (MPZ), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa95422
Clone ID Related Accession (Same CDS sequence)	NM_001316359.1
Accession Version	NM_001316359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Bos taurus(cattle)
Product	myelin protein P0 isoform L-MPZ precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EE369960.1 and BC122659.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MPZ, also known as P0) results from translation termination at the upstream UAG stop codon, while the longer isoform (L-MPZ) results from UAG stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (L-MPZ). As the UAG stop codon has been reported to specify several alternative amino acids (glutamine, tyrosine and leucine), its location in the longer isoform is denoted by an 'X'. ##Evidence-Data-START## Transcript exon combination :: BC122659.1, DV917420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTG GGGCTCCCTC GTCCAGCCCC AGTCCTATCC TGGCTGCGCT ACTCTTCTCC 
TCCTTGGTGC TCTCCCCTGT CCAGGCCATT GTGGTCTACA CAGACAAGGA GGTCCACGGT 
GCTGTGGGCT CCCAGGTGAC CCTGTACTGC TCCTTCTGGT CCAGTGAGTG GGTTTCAGAT 
GACCTCTCCT TCACCTGGCG CTACCAGCCC GAAGGGGGCC GTGATGCCAT CTCGATCTTC 
CACTACGCCA AGGGACAGCC CTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCACCG GAAGGATGGC TCCATTGTCA TACACAACCT GGACTATGGT 
GACAACGGCA CTTTCACCTG TGACGTCAAA AACCCCCCAG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCTACGAGGT ACGGGGTGGT GCTGGGAGCC 
GTGATCGGGG GCGTCCTGGG GGTGGTGCTG TTGGCGCTGC TGCTTTTCTA CCTGATTCGG 
TACTGCTGGC TACGCAGGCA GGCGGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGC 
AAATTGCACA AGACTGCGAA GGACGCCTCA AAGCGCGGCC GGCAGACGCC GGTTCTGTAT 
GCCATGCTGG ACCACAGCAG AAGCACCAAA GCTGCCAGTG AGAAGAAGAC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAGCGG TTAGCGGGCC GGGCGGGGGA TCGGGGGTTA 
GGGGCGGAGG CTTCCAAAGG CCCTCGGGTG GTGGTCATCG AGATGGAGCT ACGGAAGGAT 
GAGCAGAGCG CGGAGCTCCG GCCTGCCGTC AAGTCCCCCA GCAGAACCAG CCTCAAGAAC 
GCCCTCAAGA ACATGATGGG CCTGGACTCA GAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303288.1
CDS	51..989
Translation	MAPGAPSSSPSPILAALLFSSLVLSPVQAIVVYTDKEVHGAVGSQVTLYCSFWSSEWVSDDLSFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPHRKDGSIVIHNLDYGDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGAVIGGVLGVVLLALLLFYLIRYCWLRRQAALQRRLSAMEKGKLHKTAKDASKRGRQTPVLYAMLDHSRSTKAASEKKTKGLGESRKDKK*RLAGRAGDRGLGAEASKGPRVVVIEMELRKDEQSAELRPAVKSPSRTSLKNALKNMMGLDSEK

Target ORF information:

RefSeq Version	NM_001316359.1
Organism	Bos taurus(cattle)
Definition	Bos taurus myelin protein zero (MPZ), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316359.1

ORF Insert Sequence:

ATGGCTCCTG GGGCTCCCTC GTCCAGCCCC AGTCCTATCC TGGCTGCGCT ACTCTTCTCC 
TCCTTGGTGC TCTCCCCTGT CCAGGCCATT GTGGTCTACA CAGACAAGGA GGTCCACGGT 
GCTGTGGGCT CCCAGGTGAC CCTGTACTGC TCCTTCTGGT CCAGTGAGTG GGTTTCAGAT 
GACCTCTCCT TCACCTGGCG CTACCAGCCC GAAGGGGGCC GTGATGCCAT CTCGATCTTC 
CACTACGCCA AGGGACAGCC CTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCACCG GAAGGATGGC TCCATTGTCA TACACAACCT GGACTATGGT 
GACAACGGCA CTTTCACCTG TGACGTCAAA AACCCCCCAG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCTACGAGGT ACGGGGTGGT GCTGGGAGCC 
GTGATCGGGG GCGTCCTGGG GGTGGTGCTG TTGGCGCTGC TGCTTTTCTA CCTGATTCGG 
TACTGCTGGC TACGCAGGCA GGCGGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGC 
AAATTGCACA AGACTGCGAA GGACGCCTCA AAGCGCGGCC GGCAGACGCC GGTTCTGTAT 
GCCATGCTGG ACCACAGCAG AAGCACCAAA GCTGCCAGTG AGAAGAAGAC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAGCGG TTAGCGGGCC GGGCGGGGGA TCGGGGGTTA 
GGGGCGGAGG CTTCCAAAGG CCCTCGGGTG GTGGTCATCG AGATGGAGCT ACGGAAGGAT 
GAGCAGAGCG CGGAGCTCCG GCCTGCCGTC AAGTCCCCCA GCAGAACCAG CCTCAAGAAC 
GCCCTCAAGA ACATGATGGG CCTGGACTCA GAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon are Essential for Translational Readthrough to Produce L-MPZ.
Neurochemical research43(1)227-237(2018 Jan)
Yamaguchi Y,Baba H

L-MPZ, a novel isoform of myelin P0, is produced by stop codon readthrough.
The Journal of biological chemistry287(21)17765-76(2012 May)
Yamaguchi Y,Hayashi A,Campagnoni CW,Kimura A,Inuzuka T,Baba H

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Nucleic acids research29(23)4767-82(2001 Dec)
Beier H,Grimm M

Complete amino acid sequence of PO protein in bovine peripheral nerve myelin.
The Journal of biological chemistry262(9)4208-14(1987 Mar)
Sakamoto Y,Kitamura K,Yoshimura K,Nishijima T,Uyemura K

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MPZ cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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