PPP1R12A cDNA ORF clone, Bos taurus(cattle)

The following PPP1R12A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R12A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa41017 NM_001102217.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19031 XM_005206032.2
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19033 XM_005206034.2
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa19034 XM_005206035.2
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa234518 XM_024991984.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa234519 XM_024991985.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa234520 XM_024991986.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa234521 XM_024991987.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa234522 XM_024991988.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa90693 XM_015470877.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa234515 XM_024991981.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa234516 XM_024991982.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa234517 XM_024991983.1
Latest version!
Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa41017
    Clone ID Related Accession (Same CDS sequence) NM_001102217.1
    Accession Version NM_001102217.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC149332.1. On Aug 21, 2007 this sequence version replaced XM_865032.2. ##Evidence-Data-START## Transcript exon combination :: BC149332.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCGGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAAAC AATAGGGAGG AGTCGTTCCA CCCGAACCAG
    AGAACAAGAA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095687.1
    CDS9..2183
    Translation

    Target ORF information:

    RefSeq Version NM_001102217.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102217.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCGGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAAAC AATAGGGAGG AGTCGTTCCA CCCGAACCAG
    AGAACAAGAA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19031
    Clone ID Related Accession (Same CDS sequence) XM_005206032.2
    Accession Version XM_005206032.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103914.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005206032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAACA ATAGGGAGGA GTCGTTCCAC CCGAACCAGA
    GAACAAGAAA ATGAAGAAAA AGAGGAAAAA GAGAAACAGG ATAAAGAAAA GCAGGAAGAA
    AAGAAGGAAT CAGAAACATC TAGAGAAGAT GAATATAAAC AAAAGTACTC CAGAACATAT
    GATGAGGCTT ATCAACGCTA CAGACCAGTA TCAACTTCAA GTTCAACCAC TCCCTCCTCT
    TTACTTTCTA CAATGAGCAG TTCACTTTAT GCTTCAAGTC AATTAAACAG GCCAAATAGT
    CTTGTAGGTA TAACTTCTGC TTACTCCAGA GGATTAACTA AGGAAAATGA AAGAGAGGGA
    GAAAAGAAAG AAGAAGAAAA AGAGGGGGAA GATAAATCAC AACCTAAATC AATCAGAGAA
    CGAAGGCGAC CAAGAGAGAA AAGAAGATCT ACGGGAGTTT CGTTTTGGAC ACAAGATAGT
    GATGAAAATG AACAAGAACA ACAATCTGAT ACAGAGGAGG GATCCAATAA GAAAGAAGTT
    CAGACGGATT CCATTTCTAG ATATGAAATC AGTTCTACAT CAGCTAGTGA TCGATACGAT
    TCCTTGCTGG GTCGCTCTGG ATCATACAGT TACTCAGAAG AAAGAAAACC TTACAGTAGC
    AGGCTAGAAA AGGATGATTC AACTGACTTT AAAAAGCTTT ATGAACAAAT TCTAGCTGAA
    AATGAAAAGC TGAAAGCGCA GCTCCATGAT ACAAATATGG AACTGACAGA TCTTAAATTA
    CAATTGGAAA AAGCCACCCA GAGACAAGAA AGATTCGCTG ATAGGTCACT ATTAGAAATG
    GAAAAAAGGG TAAGTGTTGA TCTTAATGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206089.1
    CDS251..3223
    Translation

    Target ORF information:

    RefSeq Version XM_005206032.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206032.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAACA ATAGGGAGGA GTCGTTCCAC CCGAACCAGA
    GAACAAGAAA ATGAAGAAAA AGAGGAAAAA GAGAAACAGG ATAAAGAAAA GCAGGAAGAA
    AAGAAGGAAT CAGAAACATC TAGAGAAGAT GAATATAAAC AAAAGTACTC CAGAACATAT
    GATGAGGCTT ATCAACGCTA CAGACCAGTA TCAACTTCAA GTTCAACCAC TCCCTCCTCT
    TTACTTTCTA CAATGAGCAG TTCACTTTAT GCTTCAAGTC AATTAAACAG GCCAAATAGT
    CTTGTAGGTA TAACTTCTGC TTACTCCAGA GGATTAACTA AGGAAAATGA AAGAGAGGGA
    GAAAAGAAAG AAGAAGAAAA AGAGGGGGAA GATAAATCAC AACCTAAATC AATCAGAGAA
    CGAAGGCGAC CAAGAGAGAA AAGAAGATCT ACGGGAGTTT CGTTTTGGAC ACAAGATAGT
    GATGAAAATG AACAAGAACA ACAATCTGAT ACAGAGGAGG GATCCAATAA GAAAGAAGTT
    CAGACGGATT CCATTTCTAG ATATGAAATC AGTTCTACAT CAGCTAGTGA TCGATACGAT
    TCCTTGCTGG GTCGCTCTGG ATCATACAGT TACTCAGAAG AAAGAAAACC TTACAGTAGC
    AGGCTAGAAA AGGATGATTC AACTGACTTT AAAAAGCTTT ATGAACAAAT TCTAGCTGAA
    AATGAAAAGC TGAAAGCGCA GCTCCATGAT ACAAATATGG AACTGACAGA TCTTAAATTA
    CAATTGGAAA AAGCCACCCA GAGACAAGAA AGATTCGCTG ATAGGTCACT ATTAGAAATG
    GAAAAAAGGG TAAGTGTTGA TCTTAATGAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19033
    Clone ID Related Accession (Same CDS sequence) XM_005206034.2
    Accession Version XM_005206034.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2805bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103914.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005206034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTACCT CAAATCGTTT GTGGGCTGAG
    GATAGTACTG AAAAGGAAAA GGACAGTGTT CCTACGGCAG TGACCATTCC TGTTGCTCCA
    ACTGTTGTTA ATGCTGCAGC TTCTACCACA ACCCTGACTA CAACTACTGC TGGCACTGTC
    TCCTCCACTG CAGAGGTCAG GGAGAGACGC AGATCATACC TCACTCCTGT TAGAGATGAA
    GAGTCTGAAT CCCAAAGAAA AGCAAGATCT AGACAAGCAA GACAGTCTAG AAGATCAACA
    CAGGGGGTAA CATTGACTGA TCTTCAGGAA GCTGAAAAAA CAATAGGGAG GAGTCGTTCC
    ACCCGAACCA GAGAACAAGA AAATGAAGAA AAAGAGGAAA AAGAGAAACA GGATAAAGAA
    AAGCAGGAAG AAAAGAAGGA ATCAGAAACA TCTAGAGAAG ATGAATATAA ACAAAAGTAC
    TCCAGAACAT ATGATGAGGC TTATCAACGC TACAGACCAG TATCAACTTC AAGTTCAACC
    ACTCCCTCCT CTTTACTTTC TACAATGAGC AGTTCACTTT ATGCTTCAAG TCAATTAAAC
    AGGCCAAATA GTCTTGTAGG TATAACTTCT GCTTACTCCA GAGGATTAAC TAAGGAAAAT
    GAAAGAGAGG GAGAAAAGAA AGAAGAAGAA AAAGAGGGGG AAGATAAATC ACAACCTAAA
    TCAATCAGAG AACGAAGGCG ACCAAGAGAG AAAAGAAGAT CTACGGGAGT TTCGTTTTGG
    ACACAAGATA GTGATGAAAA TGAACAAGAA CAACAATCTG ATACAGAGGA GGGATCCAAT
    AAGAAAGAAG TTCAGACGGA TTCCATTTCT AGATATGAAA TCAGTTCTAC ATCAGCTAGT
    GATCGATACG ATTCCTTGCT GGGTCGCTCT GGATCATACA GTTACTCAGA AGAAAGAAAA
    CCTTACAGTA GCAGGCTAGA AAAGGATGAT TCAACTGACT TTAAAAAGCT TTATGAACAA
    ATTCTAGCTG AAAATGAAAA GCTGAAAGCG CAGCTCCATG ATACAAATAT GGAACTGACA
    GATCTTAAAT TACAATTGGA AAAAGCCACC CAGAGACAAG AAAGATTCGC TGATAGGTCA
    CTATTAGAAA TGGAAAAAAG GGTAAGTGTT GATCTTAATG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206091.1
    CDS251..3055
    Translation

    Target ORF information:

    RefSeq Version XM_005206034.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206034.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTACCT CAAATCGTTT GTGGGCTGAG
    GATAGTACTG AAAAGGAAAA GGACAGTGTT CCTACGGCAG TGACCATTCC TGTTGCTCCA
    ACTGTTGTTA ATGCTGCAGC TTCTACCACA ACCCTGACTA CAACTACTGC TGGCACTGTC
    TCCTCCACTG CAGAGGTCAG GGAGAGACGC AGATCATACC TCACTCCTGT TAGAGATGAA
    GAGTCTGAAT CCCAAAGAAA AGCAAGATCT AGACAAGCAA GACAGTCTAG AAGATCAACA
    CAGGGGGTAA CATTGACTGA TCTTCAGGAA GCTGAAAAAA CAATAGGGAG GAGTCGTTCC
    ACCCGAACCA GAGAACAAGA AAATGAAGAA AAAGAGGAAA AAGAGAAACA GGATAAAGAA
    AAGCAGGAAG AAAAGAAGGA ATCAGAAACA TCTAGAGAAG ATGAATATAA ACAAAAGTAC
    TCCAGAACAT ATGATGAGGC TTATCAACGC TACAGACCAG TATCAACTTC AAGTTCAACC
    ACTCCCTCCT CTTTACTTTC TACAATGAGC AGTTCACTTT ATGCTTCAAG TCAATTAAAC
    AGGCCAAATA GTCTTGTAGG TATAACTTCT GCTTACTCCA GAGGATTAAC TAAGGAAAAT
    GAAAGAGAGG GAGAAAAGAA AGAAGAAGAA AAAGAGGGGG AAGATAAATC ACAACCTAAA
    TCAATCAGAG AACGAAGGCG ACCAAGAGAG AAAAGAAGAT CTACGGGAGT TTCGTTTTGG
    ACACAAGATA GTGATGAAAA TGAACAAGAA CAACAATCTG ATACAGAGGA GGGATCCAAT
    AAGAAAGAAG TTCAGACGGA TTCCATTTCT AGATATGAAA TCAGTTCTAC ATCAGCTAGT
    GATCGATACG ATTCCTTGCT GGGTCGCTCT GGATCATACA GTTACTCAGA AGAAAGAAAA
    CCTTACAGTA GCAGGCTAGA AAAGGATGAT TCAACTGACT TTAAAAAGCT TTATGAACAA
    ATTCTAGCTG AAAATGAAAA GCTGAAAGCG CAGCTCCATG ATACAAATAT GGAACTGACA
    GATCTTAAAT TACAATTGGA AAAAGCCACC CAGAGACAAG AAAGATTCGC TGATAGGTCA
    CTATTAGAAA TGGAAAAAAG GGTAAGTGTT GATCTTAATG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19034
    Clone ID Related Accession (Same CDS sequence) XM_005206035.2
    Accession Version XM_005206035.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103914.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005206035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGATCATAC CTCACTCCTG TTAGAGATGA AGAGTCTGAA
    TCCCAAAGAA AAGCAAGATC TAGACAAGCA AGACAGTCTA GAAGATCAAC ACAGGGGGTA
    ACATTGACTG ATCTTCAGGA AGCTGAAAAA ACAATAGGGA GGAGTCGTTC CACCCGAACC
    AGAGAACAAG AAAATGAAGA AAAAGAGGAA AAAGAGAAAC AGGATAAAGA AAAGCAGGAA
    GAAAAGAAGG AATCAGAAAC ATCTAGAGAA GATGAATATA AACAAAAGTA CTCCAGAACA
    TATGATGAGG CTTATCAACG CTACAGACCA GTATCAACTT CAAGTTCAAC CACTCCCTCC
    TCTTTACTTT CTACAATGAG CAGTTCACTT TATGCTTCAA GTCAATTAAA CAGGCCAAAT
    AGTCTTGTAG GTATAACTTC TGCTTACTCC AGAGGATTAA CTAAGGAAAA TGAAAGAGAG
    GGAGAAAAGA AAGAAGAAGA AAAAGAGGGG GAAGATAAAT CACAACCTAA ATCAATCAGA
    GAACGAAGGC GACCAAGAGA GAAAAGAAGA TCTACGGGAG TTTCGTTTTG GACACAAGAT
    AGTGATGAAA ATGAACAAGA ACAACAATCT GATACAGAGG AGGGATCCAA TAAGAAAGAA
    GTTCAGACGG ATTCCATTTC TAGATATGAA ATCAGTTCTA CATCAGCTAG TGATCGATAC
    GATTCCTTGC TGGGTCGCTC TGGATCATAC AGTTACTCAG AAGAAAGAAA ACCTTACAGT
    AGCAGGCTAG AAAAGGATGA TTCAACTGAC TTTAAAAAGC TTTATGAACA AATTCTAGCT
    GAAAATGAAA AGCTGAAAGC GCAGCTCCAT GATACAAATA TGGAACTGAC AGATCTTAAA
    TTACAATTGG AAAAAGCCAC CCAGAGACAA GAAAGATTCG CTGATAGGTC ACTATTAGAA
    ATGGAAAAAA GGGTAAGTGT TGATCTTAAT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206092.1
    CDS251..3046
    Translation

    Target ORF information:

    RefSeq Version XM_005206035.2
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206035.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGATCATAC CTCACTCCTG TTAGAGATGA AGAGTCTGAA
    TCCCAAAGAA AAGCAAGATC TAGACAAGCA AGACAGTCTA GAAGATCAAC ACAGGGGGTA
    ACATTGACTG ATCTTCAGGA AGCTGAAAAA ACAATAGGGA GGAGTCGTTC CACCCGAACC
    AGAGAACAAG AAAATGAAGA AAAAGAGGAA AAAGAGAAAC AGGATAAAGA AAAGCAGGAA
    GAAAAGAAGG AATCAGAAAC ATCTAGAGAA GATGAATATA AACAAAAGTA CTCCAGAACA
    TATGATGAGG CTTATCAACG CTACAGACCA GTATCAACTT CAAGTTCAAC CACTCCCTCC
    TCTTTACTTT CTACAATGAG CAGTTCACTT TATGCTTCAA GTCAATTAAA CAGGCCAAAT
    AGTCTTGTAG GTATAACTTC TGCTTACTCC AGAGGATTAA CTAAGGAAAA TGAAAGAGAG
    GGAGAAAAGA AAGAAGAAGA AAAAGAGGGG GAAGATAAAT CACAACCTAA ATCAATCAGA
    GAACGAAGGC GACCAAGAGA GAAAAGAAGA TCTACGGGAG TTTCGTTTTG GACACAAGAT
    AGTGATGAAA ATGAACAAGA ACAACAATCT GATACAGAGG AGGGATCCAA TAAGAAAGAA
    GTTCAGACGG ATTCCATTTC TAGATATGAA ATCAGTTCTA CATCAGCTAG TGATCGATAC
    GATTCCTTGC TGGGTCGCTC TGGATCATAC AGTTACTCAG AAGAAAGAAA ACCTTACAGT
    AGCAGGCTAG AAAAGGATGA TTCAACTGAC TTTAAAAAGC TTTATGAACA AATTCTAGCT
    GAAAATGAAA AGCTGAAAGC GCAGCTCCAT GATACAAATA TGGAACTGAC AGATCTTAAA
    TTACAATTGG AAAAAGCCAC CCAGAGACAA GAAAGATTCG CTGATAGGTC ACTATTAGAA
    ATGGAAAAAA GGGTAAGTGT TGATCTTAAT GAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234518
    Clone ID Related Accession (Same CDS sequence) XM_024991984.1
    Accession Version XM_024991984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTACCT CAAATCGTTT GTGGGCTGAG
    GATAGTACTG AAAAGGAAAA GGACAGTGTT CCTACGGCAG TGACCATTCC TGTTGCTCCA
    ACTGTTGTTA ATGCTGCAGC TTCTACCACA ACCCTGACTA CAACTACTGC TGGCACTGTC
    TCCTCCACTG CAGAGGTCAG GGAGAGACGC AGATCATACC TCACTCCTGT TAGAGATGAA
    GAGTCTGAAT CCCAAAGAAA AGCAAGATCT AGACAAGCAA GACAGTCTAG AAGATCAACA
    CAGGGGGTAA CATTGACTGA TCTTCAGGAA GCTGAAAAAA CAATAGGGAG GAGTCGTTCC
    ACCCGAACCA GAGAACAAGA AAATGAAGAA AAAGAGGAAA AAGAGAAACA GGATAAAGAA
    AAGCAGGAAG AAAAGAAGGA ATCAGAAACA TCTAGAGAAG ATGAATATAA ACAAAAGTAC
    TCCAGAACAT ATGATGAGGC TTATCAACGC TACAGACCAG TATCAACTTC AAGTTCAACC
    ACTCCCTCCT CTTTACTTTC TACAATGAGC AGTTCACTTT ATGCTTCAAG TCAATTAAAC
    AGGCCAAATA GTCTTGTAGG TATAACTTCT GCTTACTCCA GAGGATTAAC TAAGGAAAAT
    GAAAGAGAGG GAGAAAAGAA AGAAGAAGAA AAAGAGGGGG AAGATAAATC ACAACCTAAA
    TCAATCAGAG AACGAAGGCG ACCAAGAGAG AAAAGAAGAT CTACGGGAGT TTCGTTTTGG
    ACACAAGATA GTGATGAAAA TGAACAAGAA CAACAATCTG ATACAGAGGA GGGATCCAAT
    AAGAAAGAAG TTCAGACGGA TTCCATTTCT AGATATGAAA TCAGTTCTAC ATCAGCTAGT
    GATCGATACG ATTCCTTGCT GGGTCGCTCT GGATCATACA GTTACTCAGA AGAAAGAAAA
    CCTTACAGTA GCAGGCTAGA AAAGGATGAT TCAACTGACT TTAAAAAGCT TTATGAACAA
    ATTCTAGCTG AAAATGAAAA GCTGAAAGCG CAGCTCCATG ATACAAATAT GGAACTGACA
    GATCTTAAAT TACAATTGGA AAAAGCCACC CAGAGACAAG AAAGATTCGC TGATAGGTCA
    CTATTAGAAA TGGAAAAAAG GGAACGAAGA GCTCTAGAAA GAAGAATATC TGAAATGGAA
    GAAGAGCTGA AAATGTTACC AGACCTGAAA GCAGACAACC AGAGGCTAAA AGATGAAAAT
    GGGGCCTTGA TCAGAGTTAT AAGCAAACTT TCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847752.1
    CDS251..3169
    Translation

    Target ORF information:

    RefSeq Version XM_024991984.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTACCT CAAATCGTTT GTGGGCTGAG
    GATAGTACTG AAAAGGAAAA GGACAGTGTT CCTACGGCAG TGACCATTCC TGTTGCTCCA
    ACTGTTGTTA ATGCTGCAGC TTCTACCACA ACCCTGACTA CAACTACTGC TGGCACTGTC
    TCCTCCACTG CAGAGGTCAG GGAGAGACGC AGATCATACC TCACTCCTGT TAGAGATGAA
    GAGTCTGAAT CCCAAAGAAA AGCAAGATCT AGACAAGCAA GACAGTCTAG AAGATCAACA
    CAGGGGGTAA CATTGACTGA TCTTCAGGAA GCTGAAAAAA CAATAGGGAG GAGTCGTTCC
    ACCCGAACCA GAGAACAAGA AAATGAAGAA AAAGAGGAAA AAGAGAAACA GGATAAAGAA
    AAGCAGGAAG AAAAGAAGGA ATCAGAAACA TCTAGAGAAG ATGAATATAA ACAAAAGTAC
    TCCAGAACAT ATGATGAGGC TTATCAACGC TACAGACCAG TATCAACTTC AAGTTCAACC
    ACTCCCTCCT CTTTACTTTC TACAATGAGC AGTTCACTTT ATGCTTCAAG TCAATTAAAC
    AGGCCAAATA GTCTTGTAGG TATAACTTCT GCTTACTCCA GAGGATTAAC TAAGGAAAAT
    GAAAGAGAGG GAGAAAAGAA AGAAGAAGAA AAAGAGGGGG AAGATAAATC ACAACCTAAA
    TCAATCAGAG AACGAAGGCG ACCAAGAGAG AAAAGAAGAT CTACGGGAGT TTCGTTTTGG
    ACACAAGATA GTGATGAAAA TGAACAAGAA CAACAATCTG ATACAGAGGA GGGATCCAAT
    AAGAAAGAAG TTCAGACGGA TTCCATTTCT AGATATGAAA TCAGTTCTAC ATCAGCTAGT
    GATCGATACG ATTCCTTGCT GGGTCGCTCT GGATCATACA GTTACTCAGA AGAAAGAAAA
    CCTTACAGTA GCAGGCTAGA AAAGGATGAT TCAACTGACT TTAAAAAGCT TTATGAACAA
    ATTCTAGCTG AAAATGAAAA GCTGAAAGCG CAGCTCCATG ATACAAATAT GGAACTGACA
    GATCTTAAAT TACAATTGGA AAAAGCCACC CAGAGACAAG AAAGATTCGC TGATAGGTCA
    CTATTAGAAA TGGAAAAAAG GGAACGAAGA GCTCTAGAAA GAAGAATATC TGAAATGGAA
    GAAGAGCTGA AAATGTTACC AGACCTGAAA GCAGACAACC AGAGGCTAAA AGATGAAAAT
    GGGGCCTTGA TCAGAGTTAT AAGCAAACTT TCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234519
    Clone ID Related Accession (Same CDS sequence) XM_024991985.1
    Accession Version XM_024991985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGATCATAC CTCACTCCTG TTAGAGATGA AGAGTCTGAA
    TCCCAAAGAA AAGCAAGATC TAGACAAGCA AGACAGTCTA GAAGATCAAC ACAGGGGGTA
    ACATTGACTG ATCTTCAGGA AGCTGAAAAA ACAATAGGGA GGAGTCGTTC CACCCGAACC
    AGAGAACAAG AAAATGAAGA AAAAGAGGAA AAAGAGAAAC AGGATAAAGA AAAGCAGGAA
    GAAAAGAAGG AATCAGAAAC ATCTAGAGAA GATGAATATA AACAAAAGTA CTCCAGAACA
    TATGATGAGG CTTATCAACG CTACAGACCA GTATCAACTT CAAGTTCAAC CACTCCCTCC
    TCTTTACTTT CTACAATGAG CAGTTCACTT TATGCTTCAA GTCAATTAAA CAGGCCAAAT
    AGTCTTGTAG GTATAACTTC TGCTTACTCC AGAGGATTAA CTAAGGAAAA TGAAAGAGAG
    GGAGAAAAGA AAGAAGAAGA AAAAGAGGGG GAAGATAAAT CACAACCTAA ATCAATCAGA
    GAACGAAGGC GACCAAGAGA GAAAAGAAGA TCTACGGGAG TTTCGTTTTG GACACAAGAT
    AGTGATGAAA ATGAACAAGA ACAACAATCT GATACAGAGG AGGGATCCAA TAAGAAAGAA
    GTTCAGACGG ATTCCATTTC TAGATATGAA ATCAGTTCTA CATCAGCTAG TGATCGATAC
    GATTCCTTGC TGGGTCGCTC TGGATCATAC AGTTACTCAG AAGAAAGAAA ACCTTACAGT
    AGCAGGCTAG AAAAGGATGA TTCAACTGAC TTTAAAAAGC TTTATGAACA AATTCTAGCT
    GAAAATGAAA AGCTGAAAGC GCAGCTCCAT GATACAAATA TGGAACTGAC AGATCTTAAA
    TTACAATTGG AAAAAGCCAC CCAGAGACAA GAAAGATTCG CTGATAGGTC ACTATTAGAA
    ATGGAAAAAA GGGAACGAAG AGCTCTAGAA AGAAGAATAT CTGAAATGGA AGAAGAGCTG
    AAAATGTTAC CAGACCTGAA AGCAGACAAC CAGAGGCTAA AAGATGAAAA TGGGGCCTTG
    ATCAGAGTTA TAAGCAAACT TTCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847753.1
    CDS251..3160
    Translation

    Target ORF information:

    RefSeq Version XM_024991985.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGATCATAC CTCACTCCTG TTAGAGATGA AGAGTCTGAA
    TCCCAAAGAA AAGCAAGATC TAGACAAGCA AGACAGTCTA GAAGATCAAC ACAGGGGGTA
    ACATTGACTG ATCTTCAGGA AGCTGAAAAA ACAATAGGGA GGAGTCGTTC CACCCGAACC
    AGAGAACAAG AAAATGAAGA AAAAGAGGAA AAAGAGAAAC AGGATAAAGA AAAGCAGGAA
    GAAAAGAAGG AATCAGAAAC ATCTAGAGAA GATGAATATA AACAAAAGTA CTCCAGAACA
    TATGATGAGG CTTATCAACG CTACAGACCA GTATCAACTT CAAGTTCAAC CACTCCCTCC
    TCTTTACTTT CTACAATGAG CAGTTCACTT TATGCTTCAA GTCAATTAAA CAGGCCAAAT
    AGTCTTGTAG GTATAACTTC TGCTTACTCC AGAGGATTAA CTAAGGAAAA TGAAAGAGAG
    GGAGAAAAGA AAGAAGAAGA AAAAGAGGGG GAAGATAAAT CACAACCTAA ATCAATCAGA
    GAACGAAGGC GACCAAGAGA GAAAAGAAGA TCTACGGGAG TTTCGTTTTG GACACAAGAT
    AGTGATGAAA ATGAACAAGA ACAACAATCT GATACAGAGG AGGGATCCAA TAAGAAAGAA
    GTTCAGACGG ATTCCATTTC TAGATATGAA ATCAGTTCTA CATCAGCTAG TGATCGATAC
    GATTCCTTGC TGGGTCGCTC TGGATCATAC AGTTACTCAG AAGAAAGAAA ACCTTACAGT
    AGCAGGCTAG AAAAGGATGA TTCAACTGAC TTTAAAAAGC TTTATGAACA AATTCTAGCT
    GAAAATGAAA AGCTGAAAGC GCAGCTCCAT GATACAAATA TGGAACTGAC AGATCTTAAA
    TTACAATTGG AAAAAGCCAC CCAGAGACAA GAAAGATTCG CTGATAGGTC ACTATTAGAA
    ATGGAAAAAA GGGAACGAAG AGCTCTAGAA AGAAGAATAT CTGAAATGGA AGAAGAGCTG
    AAAATGTTAC CAGACCTGAA AGCAGACAAC CAGAGGCTAA AAGATGAAAA TGGGGCCTTG
    ATCAGAGTTA TAAGCAAACT TTCCAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234520
    Clone ID Related Accession (Same CDS sequence) XM_024991986.1
    Accession Version XM_024991986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTACCT CAAATCGTTT GTGGGCTGAG
    GATAGTACTG AAAAGGAAAA GGACAGTGTT CCTACGGCAG TGACCATTCC TGTTGCTCCA
    ACTGTTGTTA ATGCTGCAGC TTCTACCACA ACCCTGACTA CAACTACTGC TGGCACTGTC
    TCCTCCACTG CAGAGGTCAG GGAGAGACGC AGATCATACC TCACTCCTGT TAGAGATGAA
    GAGTCTGAAT CCCAAAGAAA AGCAAGATCT AGACAAGCAA GACAGTCTAG AAGATCAACA
    CAGGGGGTAA CATTGACTGA TCTTCAGGAA GCTGAAAAAA CAATAGGGAG GAGTCGTTCC
    ACCCGAACCA GAGAACAAGA AAATGAAGAA AAAGAGGAAA AAGAGAAACA GGATAAAGAA
    AAGCAGGAAG AAAAGAAGGA ATCAGAAACA TCTAGAGAAG ATGAATATAA ACAAAAGTAC
    TCCAGAACAT ATGATGAGGC TTATCAACGC TACAGACCAG TATCAACTTC AAGTTCAACC
    ACTCCCTCCT CTTTACTTTC TACAATGAGC AGTTCACTTT ATGCTTCAAG TCAATTAAAC
    AGGCCAAATA GTCTTGTAGG TATAACTTCT GCTTACTCCA GAGGATTAAC TAAGGAAAAT
    GAAAGAGAGG GAGAAAAGAA AGAAGAAGAA AAAGAGGGGG AAGATAAATC ACAACCTAAA
    TCAATCAGAG AACGAAGGCG ACCAAGAGAG AAAAGAAGAT CTACGGGAGT TTCGTTTTGG
    ACACAAGATA GTGATGAAAA TGAACAAGAA CAACAATCTG ATACAGAGGA GGGATCCAAT
    AAGAAAGAAG TTCAGACGGA TTCCATTTCT AGATATGAAA TCAGTTCTAC ATCAGCTAGT
    GATCGATACG ATTCCTTGCT GGGTCGCTCT GGATCATACA GTTACTCAGA AGAAAGAAAA
    CCTTACAGTA GCAGGCTAGA AAAGGATGAT TCAACTGACT TTAAAAAGCT TTATGAACAA
    ATTCTAGCTG AAAATGAAAA GCTGAAAGCG CAGCTCCATG ATACAAATAT GGAACTGACA
    GATCTTAAAT TACAATTGGA AAAAGCCACC CAGAGACAAG AAAGATTCGC TGATAGGTCA
    CTATTAGAAA TGGAAAAAAG GGTGACCGGC AAGAGTCAGT ATCTTCTCGG CGGAACGAAG
    AGCTCTAGAA AGAAGAATAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847754.1
    CDS256..3099
    Translation

    Target ORF information:

    RefSeq Version XM_024991986.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTACCT CAAATCGTTT GTGGGCTGAG
    GATAGTACTG AAAAGGAAAA GGACAGTGTT CCTACGGCAG TGACCATTCC TGTTGCTCCA
    ACTGTTGTTA ATGCTGCAGC TTCTACCACA ACCCTGACTA CAACTACTGC TGGCACTGTC
    TCCTCCACTG CAGAGGTCAG GGAGAGACGC AGATCATACC TCACTCCTGT TAGAGATGAA
    GAGTCTGAAT CCCAAAGAAA AGCAAGATCT AGACAAGCAA GACAGTCTAG AAGATCAACA
    CAGGGGGTAA CATTGACTGA TCTTCAGGAA GCTGAAAAAA CAATAGGGAG GAGTCGTTCC
    ACCCGAACCA GAGAACAAGA AAATGAAGAA AAAGAGGAAA AAGAGAAACA GGATAAAGAA
    AAGCAGGAAG AAAAGAAGGA ATCAGAAACA TCTAGAGAAG ATGAATATAA ACAAAAGTAC
    TCCAGAACAT ATGATGAGGC TTATCAACGC TACAGACCAG TATCAACTTC AAGTTCAACC
    ACTCCCTCCT CTTTACTTTC TACAATGAGC AGTTCACTTT ATGCTTCAAG TCAATTAAAC
    AGGCCAAATA GTCTTGTAGG TATAACTTCT GCTTACTCCA GAGGATTAAC TAAGGAAAAT
    GAAAGAGAGG GAGAAAAGAA AGAAGAAGAA AAAGAGGGGG AAGATAAATC ACAACCTAAA
    TCAATCAGAG AACGAAGGCG ACCAAGAGAG AAAAGAAGAT CTACGGGAGT TTCGTTTTGG
    ACACAAGATA GTGATGAAAA TGAACAAGAA CAACAATCTG ATACAGAGGA GGGATCCAAT
    AAGAAAGAAG TTCAGACGGA TTCCATTTCT AGATATGAAA TCAGTTCTAC ATCAGCTAGT
    GATCGATACG ATTCCTTGCT GGGTCGCTCT GGATCATACA GTTACTCAGA AGAAAGAAAA
    CCTTACAGTA GCAGGCTAGA AAAGGATGAT TCAACTGACT TTAAAAAGCT TTATGAACAA
    ATTCTAGCTG AAAATGAAAA GCTGAAAGCG CAGCTCCATG ATACAAATAT GGAACTGACA
    GATCTTAAAT TACAATTGGA AAAAGCCACC CAGAGACAAG AAAGATTCGC TGATAGGTCA
    CTATTAGAAA TGGAAAAAAG GGTGACCGGC AAGAGTCAGT ATCTTCTCGG CGGAACGAAG
    AGCTCTAGAA AGAAGAATAT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234521
    Clone ID Related Accession (Same CDS sequence) XM_024991987.1
    Accession Version XM_024991987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTGAAGT TTCTGGTAGA AAATGGAGCA AATATTAATC AACCTGATAA TGAAGGCTGG 
    ATACCTCTGC ATGCAGCTGC TTCCTGTGGA TATCTTGATA TTGCAGAGTT TTTGATTGGT
    CAGGGAGCAC ATGTAGGAGC TGTCAACAGT GAAGGAGACA CACCTTTAGA TATCGCTGAG
    GAGGAGGCAA TGGAAGAGCT ACTGCAGAAT GAAGTTAATC GGCAAGGGGT TGATATAGAA
    GCAGCTCGAA AAGAAGAGGA ACGAATAATG CTTAGAGATG CCAGACAGTG GTTAAATAGT
    GGTCATATAA ATGATGTCCG GCATGCAAAA TCTGGAGGTA CAGCGCTTCA CGTTGCAGCC
    GCCAAGGGCT ATACAGAAGT TTTAAAACTT TTAATACAGG CAGGCTATGA TGTTAATATT
    AAAGATTATG ATGGCTGGAC ACCTCTTCAT GCTGCAGCTC ATTGGGGTAA AGAAGAAGCA
    TGTCGGATTT TAGTGGACAA TCTGTGTGAT ATGGAGATGG TCAACAAAGT GGGCCAGACA
    GCCTTTGATG TAGCAGATGA GGACATTTTA GGATATTTAG AAGAGTTGCA AAAGAAGCAA
    AATCTGCTCC ATAGTGAAAA ACGGGAAAAG AAATCTCCAC TAATTGAATC AACAGCTAAT
    ATGGATAATA ATCAGTCACA GAAGACCTTT AAAAATAAAG AGACATTGAT TATTGAGCCT
    GAGAAAAATG CATCCCGTAT TGAATCTCTG GAACAAGAAA AGGTTGATGA TGAAGAAGAA
    GGAAAGAAAG ATGAATCTAG TTGCTCTAGT GAAGAAGATG AGGAAGATGA CTCAGAATCA
    GAAGCTGAAA CAGATAAGAC AAAACCCATG GCTTCTGTAA CTAATGCCAA TACTTCTAGT
    ACACAAGCAG CTCCTGTAGC TGTTACAACA CCTACTGTGT CATCTGGTCA GCCAACACCT
    ACATCACCTA TTAAAAAGTT TCCAGCTTCA GCTACAAAAA TTTCTCCCAA AGAAGAAGAG
    AGAAAAGATG AGTCCCCTGC ATCTTGGAGG TTAGGACTTA GAAAGACGGG CAGTTACGGT
    GCACTTGCAG AAATCACAGC ATCTAAAGAA GCTCAGAAGG AAAAAGACAC TGCAGGTGTC
    ATGCGCTCAG CTTCCAGTCC CAGACTTTCC TCCTCTTTGG ATAATAAAGA AAAGGAGAAA
    GATAGTAAAG GAACTAAGCT TGCATATGTT ACGCCTACAA TACCAAGGCG GCTTGCCAGT
    ACATCTGACA TTGAAGAGAA AGAAAACAGA GATTCTTCAA GTTTGCGAAC AAGTAGTTCA
    TACACAAGAA GAAAATGGGA AGATGATCTT AAAAAGAATA GCTCAATTAA TGAAGGATCA
    ACTTATCACA AAAGTTGCTC CTTTGGTAGA AGACAAGATG ATTTGATTAG TTCTAGTGTT
    CCAAGCACCA CATCAACACC AACAGTTACC TCTGCAGCTG GGCTTCAGAA AAGCCTGCTT
    TCCAGCACAA GCACTACTAC AAAGATTACA ACGGGTTCTT CCTCAGCAGG CACACAAAGC
    AGTACCTCAA ATCGTTTGTG GGCTGAGGAT AGTACTGAAA AGGAAAAGGA CAGTGTTCCT
    ACGGCAGTGA CCATTCCTGT TGCTCCAACT GTTGTTAATG CTGCAGCTTC TACCACAACC
    CTGACTACAA CTACTGCTGG CACTGTCTCC TCCACTGCAG AGGTCAGGGA GAGACGCAGA
    TCATACCTCA CTCCTGTTAG AGATGAAGAG TCTGAATCCC AAAGAAAAGC AAGATCTAGA
    CAAGCAAGAC AGTCTAGAAG ATCAACACAG GGGGTAACAT TGACTGATCT TCAGGAAGCT
    GAAAAAACAA TAGGGAGGAG TCGTTCCACC CGAACCAGAG AACAAGAAAA TGAAGAAAAA
    GAGGAAAAAG AGAAACAGGA TAAAGAAAAG CAGGAAGAAA AGAAGGAATC AGAAACATCT
    AGAGAAGATG AATATAAACA AAAGTACTCC AGAACATATG ATGAGGCTTA TCAACGCTAC
    AGACCAGTAT CAACTTCAAG TTCAACCACT CCCTCCTCTT TACTTTCTAC AATGAGCAGT
    TCACTTTATG CTTCAAGTCA ATTAAACAGG CCAAATAGTC TTGTAGGTAT AACTTCTGCT
    TACTCCAGAG GATTAACTAA GGAAAATGAA AGAGAGGGAG AAAAGAAAGA AGAAGAAAAA
    GAGGGGGAAG ATAAATCACA ACCTAAATCA ATCAGAGAAC GAAGGCGACC AAGAGAGAAA
    AGAAGATCTA CGGGAGTTTC GTTTTGGACA CAAGATAGTG ATGAAAATGA ACAAGAACAA
    CAATCTGATA CAGAGGAGGG ATCCAATAAG AAAGAAGTTC AGACGGATTC CATTTCTAGA
    TATGAAATCA GTTCTACATC AGCTAGTGAT CGATACGATT CCTTGCTGGG TCGCTCTGGA
    TCATACAGTT ACTCAGAAGA AAGAAAACCT TACAGTAGCA GGCTAGAAAA GGATGATTCA
    ACTGACTTTA AAAAGCTTTA TGAACAAATT CTAGCTGAAA ATGAAAAGCT GAAAGCGCAG
    CTCCATGATA CAAATATGGA ACTGACAGAT CTTAAATTAC AATTGGAAAA AGCCACCCAG
    AGACAAGAAA GATTCGCTGA TAGGTCACTA TTAGAAATGG AAAAAAGGGA ACGAAGAGCT
    CTAGAAAGAA GAATATCTGA AATGGAAGAA GAGCTGAAAA TGTTACCAGA CCTGAAAGCA
    GACAACCAGA GGCTAAAAGA TGAAAATGGG GCCTTGATCA GAGTTATAAG CAAACTTTCC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847755.1
    CDS203..3028
    Translation

    Target ORF information:

    RefSeq Version XM_024991987.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGTGAAGT TTCTGGTAGA AAATGGAGCA AATATTAATC AACCTGATAA TGAAGGCTGG 
    ATACCTCTGC ATGCAGCTGC TTCCTGTGGA TATCTTGATA TTGCAGAGTT TTTGATTGGT
    CAGGGAGCAC ATGTAGGAGC TGTCAACAGT GAAGGAGACA CACCTTTAGA TATCGCTGAG
    GAGGAGGCAA TGGAAGAGCT ACTGCAGAAT GAAGTTAATC GGCAAGGGGT TGATATAGAA
    GCAGCTCGAA AAGAAGAGGA ACGAATAATG CTTAGAGATG CCAGACAGTG GTTAAATAGT
    GGTCATATAA ATGATGTCCG GCATGCAAAA TCTGGAGGTA CAGCGCTTCA CGTTGCAGCC
    GCCAAGGGCT ATACAGAAGT TTTAAAACTT TTAATACAGG CAGGCTATGA TGTTAATATT
    AAAGATTATG ATGGCTGGAC ACCTCTTCAT GCTGCAGCTC ATTGGGGTAA AGAAGAAGCA
    TGTCGGATTT TAGTGGACAA TCTGTGTGAT ATGGAGATGG TCAACAAAGT GGGCCAGACA
    GCCTTTGATG TAGCAGATGA GGACATTTTA GGATATTTAG AAGAGTTGCA AAAGAAGCAA
    AATCTGCTCC ATAGTGAAAA ACGGGAAAAG AAATCTCCAC TAATTGAATC AACAGCTAAT
    ATGGATAATA ATCAGTCACA GAAGACCTTT AAAAATAAAG AGACATTGAT TATTGAGCCT
    GAGAAAAATG CATCCCGTAT TGAATCTCTG GAACAAGAAA AGGTTGATGA TGAAGAAGAA
    GGAAAGAAAG ATGAATCTAG TTGCTCTAGT GAAGAAGATG AGGAAGATGA CTCAGAATCA
    GAAGCTGAAA CAGATAAGAC AAAACCCATG GCTTCTGTAA CTAATGCCAA TACTTCTAGT
    ACACAAGCAG CTCCTGTAGC TGTTACAACA CCTACTGTGT CATCTGGTCA GCCAACACCT
    ACATCACCTA TTAAAAAGTT TCCAGCTTCA GCTACAAAAA TTTCTCCCAA AGAAGAAGAG
    AGAAAAGATG AGTCCCCTGC ATCTTGGAGG TTAGGACTTA GAAAGACGGG CAGTTACGGT
    GCACTTGCAG AAATCACAGC ATCTAAAGAA GCTCAGAAGG AAAAAGACAC TGCAGGTGTC
    ATGCGCTCAG CTTCCAGTCC CAGACTTTCC TCCTCTTTGG ATAATAAAGA AAAGGAGAAA
    GATAGTAAAG GAACTAAGCT TGCATATGTT ACGCCTACAA TACCAAGGCG GCTTGCCAGT
    ACATCTGACA TTGAAGAGAA AGAAAACAGA GATTCTTCAA GTTTGCGAAC AAGTAGTTCA
    TACACAAGAA GAAAATGGGA AGATGATCTT AAAAAGAATA GCTCAATTAA TGAAGGATCA
    ACTTATCACA AAAGTTGCTC CTTTGGTAGA AGACAAGATG ATTTGATTAG TTCTAGTGTT
    CCAAGCACCA CATCAACACC AACAGTTACC TCTGCAGCTG GGCTTCAGAA AAGCCTGCTT
    TCCAGCACAA GCACTACTAC AAAGATTACA ACGGGTTCTT CCTCAGCAGG CACACAAAGC
    AGTACCTCAA ATCGTTTGTG GGCTGAGGAT AGTACTGAAA AGGAAAAGGA CAGTGTTCCT
    ACGGCAGTGA CCATTCCTGT TGCTCCAACT GTTGTTAATG CTGCAGCTTC TACCACAACC
    CTGACTACAA CTACTGCTGG CACTGTCTCC TCCACTGCAG AGGTCAGGGA GAGACGCAGA
    TCATACCTCA CTCCTGTTAG AGATGAAGAG TCTGAATCCC AAAGAAAAGC AAGATCTAGA
    CAAGCAAGAC AGTCTAGAAG ATCAACACAG GGGGTAACAT TGACTGATCT TCAGGAAGCT
    GAAAAAACAA TAGGGAGGAG TCGTTCCACC CGAACCAGAG AACAAGAAAA TGAAGAAAAA
    GAGGAAAAAG AGAAACAGGA TAAAGAAAAG CAGGAAGAAA AGAAGGAATC AGAAACATCT
    AGAGAAGATG AATATAAACA AAAGTACTCC AGAACATATG ATGAGGCTTA TCAACGCTAC
    AGACCAGTAT CAACTTCAAG TTCAACCACT CCCTCCTCTT TACTTTCTAC AATGAGCAGT
    TCACTTTATG CTTCAAGTCA ATTAAACAGG CCAAATAGTC TTGTAGGTAT AACTTCTGCT
    TACTCCAGAG GATTAACTAA GGAAAATGAA AGAGAGGGAG AAAAGAAAGA AGAAGAAAAA
    GAGGGGGAAG ATAAATCACA ACCTAAATCA ATCAGAGAAC GAAGGCGACC AAGAGAGAAA
    AGAAGATCTA CGGGAGTTTC GTTTTGGACA CAAGATAGTG ATGAAAATGA ACAAGAACAA
    CAATCTGATA CAGAGGAGGG ATCCAATAAG AAAGAAGTTC AGACGGATTC CATTTCTAGA
    TATGAAATCA GTTCTACATC AGCTAGTGAT CGATACGATT CCTTGCTGGG TCGCTCTGGA
    TCATACAGTT ACTCAGAAGA AAGAAAACCT TACAGTAGCA GGCTAGAAAA GGATGATTCA
    ACTGACTTTA AAAAGCTTTA TGAACAAATT CTAGCTGAAA ATGAAAAGCT GAAAGCGCAG
    CTCCATGATA CAAATATGGA ACTGACAGAT CTTAAATTAC AATTGGAAAA AGCCACCCAG
    AGACAAGAAA GATTCGCTGA TAGGTCACTA TTAGAAATGG AAAAAAGGGA ACGAAGAGCT
    CTAGAAAGAA GAATATCTGA AATGGAAGAA GAGCTGAAAA TGTTACCAGA CCTGAAAGCA
    GACAACCAGA GGCTAAAAGA TGAAAATGGG GCCTTGATCA GAGTTATAAG CAAACTTTCC
    AAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234522
    Clone ID Related Accession (Same CDS sequence) XM_024991988.1
    Accession Version XM_024991988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2742bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGATCAT ACCTCACTCC TGTTAGAGAT
    GAAGAGTCTG AATCCCAAAG AAAAGCAAGA TCTAGACAAG CAAGACAGTC TAGAAGATCA
    ACACAGGGGG TAACATTGAC TGATCTTCAG GAAGCTGAAA AAACAATAGG GAGGAGTCGT
    TCCACCCGAA CCAGAGAACA AGAAAATGAA GAAAAAGAGG AAAAAGAGAA ACAGGATAAA
    GAAAAGCAGG AAGAAAAGAA GGAATCAGAA ACATCTAGAG AAGATGAATA TAAACAAAAG
    TACTCCAGAA CATATGATGA GGCTTATCAA CGCTACAGAC CAGTATCAAC TTCAAGTTCA
    ACCACTCCCT CCTCTTTACT TTCTACAATG AGCAGTTCAC TTTATGCTTC AAGTCAATTA
    AACAGGCCAA ATAGTCTTGT AGGTATAACT TCTGCTTACT CCAGAGGATT AACTAAGGAA
    AATGAAAGAG AGGGAGAAAA GAAAGAAGAA GAAAAAGAGG GGGAAGATAA ATCACAACCT
    AAATCAATCA GAGAACGAAG GCGACCAAGA GAGAAAAGAA GATCTACGGG AGTTTCGTTT
    TGGACACAAG ATAGTGATGA AAATGAACAA GAACAACAAT CTGATACAGA GGAGGGATCC
    AATAAGAAAG AAGTTCAGAC GGATTCCATT TCTAGATATG AAATCAGTTC TACATCAGCT
    AGTGATCGAT ACGATTCCTT GCTGGGTCGC TCTGGATCAT ACAGTTACTC AGAAGAAAGA
    AAACCTTACA GTAGCAGGCT AGAAAAGGAT GATTCAACTG ACTTTAAAAA GCTTTATGAA
    CAAATTCTAG CTGAAAATGA AAAGCTGAAA GCGCAGCTCC ATGATACAAA TATGGAACTG
    ACAGATCTTA AATTACAATT GGAAAAAGCC ACCCAGAGAC AAGAAAGATT CGCTGATAGG
    TCACTATTAG AAATGGAAAA AAGGGAACGA AGAGCTCTAG AAAGAAGAAT ATCTGAAATG
    GAAGAAGAGC TGAAAATGTT ACCAGACCTG AAAGCAGACA ACCAGAGGCT AAAAGATGAA
    AATGGGGCCT TGATCAGAGT TATAAGCAAA CTTTCCAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847756.1
    CDS251..2992
    Translation

    Target ORF information:

    RefSeq Version XM_024991988.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGATCAT ACCTCACTCC TGTTAGAGAT
    GAAGAGTCTG AATCCCAAAG AAAAGCAAGA TCTAGACAAG CAAGACAGTC TAGAAGATCA
    ACACAGGGGG TAACATTGAC TGATCTTCAG GAAGCTGAAA AAACAATAGG GAGGAGTCGT
    TCCACCCGAA CCAGAGAACA AGAAAATGAA GAAAAAGAGG AAAAAGAGAA ACAGGATAAA
    GAAAAGCAGG AAGAAAAGAA GGAATCAGAA ACATCTAGAG AAGATGAATA TAAACAAAAG
    TACTCCAGAA CATATGATGA GGCTTATCAA CGCTACAGAC CAGTATCAAC TTCAAGTTCA
    ACCACTCCCT CCTCTTTACT TTCTACAATG AGCAGTTCAC TTTATGCTTC AAGTCAATTA
    AACAGGCCAA ATAGTCTTGT AGGTATAACT TCTGCTTACT CCAGAGGATT AACTAAGGAA
    AATGAAAGAG AGGGAGAAAA GAAAGAAGAA GAAAAAGAGG GGGAAGATAA ATCACAACCT
    AAATCAATCA GAGAACGAAG GCGACCAAGA GAGAAAAGAA GATCTACGGG AGTTTCGTTT
    TGGACACAAG ATAGTGATGA AAATGAACAA GAACAACAAT CTGATACAGA GGAGGGATCC
    AATAAGAAAG AAGTTCAGAC GGATTCCATT TCTAGATATG AAATCAGTTC TACATCAGCT
    AGTGATCGAT ACGATTCCTT GCTGGGTCGC TCTGGATCAT ACAGTTACTC AGAAGAAAGA
    AAACCTTACA GTAGCAGGCT AGAAAAGGAT GATTCAACTG ACTTTAAAAA GCTTTATGAA
    CAAATTCTAG CTGAAAATGA AAAGCTGAAA GCGCAGCTCC ATGATACAAA TATGGAACTG
    ACAGATCTTA AATTACAATT GGAAAAAGCC ACCCAGAGAC AAGAAAGATT CGCTGATAGG
    TCACTATTAG AAATGGAAAA AAGGGAACGA AGAGCTCTAG AAAGAAGAAT ATCTGAAATG
    GAAGAAGAGC TGAAAATGTT ACCAGACCTG AAAGCAGACA ACCAGAGGCT AAAAGATGAA
    AATGGGGCCT TGATCAGAGT TATAAGCAAA CTTTCCAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90693
    Clone ID Related Accession (Same CDS sequence) XM_015470877.1
    Accession Version XM_015470877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2628bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103914.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGATCAT ACCTCACTCC TGTTAGAGAT
    GAAGAGTCTG AATCCCAAAG AAAAGCAAGA TCTAGACAAG CAAGACAGTC TAGAAGATCA
    ACACAGGGGG TAACATTGAC TGATCTTCAG GAAGCTGAAA AAACAATAGG GAGGAGTCGT
    TCCACCCGAA CCAGAGAACA AGAAAATGAA GAAAAAGAGG AAAAAGAGAA ACAGGATAAA
    GAAAAGCAGG AAGAAAAGAA GGAATCAGAA ACATCTAGAG AAGATGAATA TAAACAAAAG
    TACTCCAGAA CATATGATGA GGCTTATCAA CGCTACAGAC CAGTATCAAC TTCAAGTTCA
    ACCACTCCCT CCTCTTTACT TTCTACAATG AGCAGTTCAC TTTATGCTTC AAGTCAATTA
    AACAGGCCAA ATAGTCTTGT AGGTATAACT TCTGCTTACT CCAGAGGATT AACTAAGGAA
    AATGAAAGAG AGGGAGAAAA GAAAGAAGAA GAAAAAGAGG GGGAAGATAA ATCACAACCT
    AAATCAATCA GAGAACGAAG GCGACCAAGA GAGAAAAGAA GATCTACGGG AGTTTCGTTT
    TGGACACAAG ATAGTGATGA AAATGAACAA GAACAACAAT CTGATACAGA GGAGGGATCC
    AATAAGAAAG AAGTTCAGAC GGATTCCATT TCTAGATATG AAATCAGTTC TACATCAGCT
    AGTGATCGAT ACGATTCCTT GCTGGGTCGC TCTGGATCAT ACAGTTACTC AGAAGAAAGA
    AAACCTTACA GTAGCAGGCT AGAAAAGGAT GATTCAACTG ACTTTAAAAA GCTTTATGAA
    CAAATTCTAG CTGAAAATGA AAAGCTGAAA GCGCAGCTCC ATGATACAAA TATGGAACTG
    ACAGATCTTA AATTACAATT GGAAAAAGCC ACCCAGAGAC AAGAAAGATT CGCTGATAGG
    TCACTATTAG AAATGGAAAA AAGGGTAAGT GTTGATCTTA ATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326363.1
    CDS251..2878
    Translation

    Target ORF information:

    RefSeq Version XM_015470877.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGATCAT ACCTCACTCC TGTTAGAGAT
    GAAGAGTCTG AATCCCAAAG AAAAGCAAGA TCTAGACAAG CAAGACAGTC TAGAAGATCA
    ACACAGGGGG TAACATTGAC TGATCTTCAG GAAGCTGAAA AAACAATAGG GAGGAGTCGT
    TCCACCCGAA CCAGAGAACA AGAAAATGAA GAAAAAGAGG AAAAAGAGAA ACAGGATAAA
    GAAAAGCAGG AAGAAAAGAA GGAATCAGAA ACATCTAGAG AAGATGAATA TAAACAAAAG
    TACTCCAGAA CATATGATGA GGCTTATCAA CGCTACAGAC CAGTATCAAC TTCAAGTTCA
    ACCACTCCCT CCTCTTTACT TTCTACAATG AGCAGTTCAC TTTATGCTTC AAGTCAATTA
    AACAGGCCAA ATAGTCTTGT AGGTATAACT TCTGCTTACT CCAGAGGATT AACTAAGGAA
    AATGAAAGAG AGGGAGAAAA GAAAGAAGAA GAAAAAGAGG GGGAAGATAA ATCACAACCT
    AAATCAATCA GAGAACGAAG GCGACCAAGA GAGAAAAGAA GATCTACGGG AGTTTCGTTT
    TGGACACAAG ATAGTGATGA AAATGAACAA GAACAACAAT CTGATACAGA GGAGGGATCC
    AATAAGAAAG AAGTTCAGAC GGATTCCATT TCTAGATATG AAATCAGTTC TACATCAGCT
    AGTGATCGAT ACGATTCCTT GCTGGGTCGC TCTGGATCAT ACAGTTACTC AGAAGAAAGA
    AAACCTTACA GTAGCAGGCT AGAAAAGGAT GATTCAACTG ACTTTAAAAA GCTTTATGAA
    CAAATTCTAG CTGAAAATGA AAAGCTGAAA GCGCAGCTCC ATGATACAAA TATGGAACTG
    ACAGATCTTA AATTACAATT GGAAAAAGCC ACCCAGAGAC AAGAAAGATT CGCTGATAGG
    TCACTATTAG AAATGGAAAA AAGGGTAAGT GTTGATCTTA ATGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234515
    Clone ID Related Accession (Same CDS sequence) XM_024991981.1
    Accession Version XM_024991981.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3087bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAACA ATAGGGAGGA GTCGTTCCAC CCGAACCAGA
    GAACAAGAAA ATGAAGAAAA AGAGGAAAAA GAGAAACAGG ATAAAGAAAA GCAGGAAGAA
    AAGAAGGAAT CAGAAACATC TAGAGAAGAT GAATATAAAC AAAAGTACTC CAGAACATAT
    GATGAGGCTT ATCAACGCTA CAGACCAGTA TCAACTTCAA GTTCAACCAC TCCCTCCTCT
    TTACTTTCTA CAATGAGCAG TTCACTTTAT GCTTCAAGTC AATTAAACAG GCCAAATAGT
    CTTGTAGGTA TAACTTCTGC TTACTCCAGA GGATTAACTA AGGAAAATGA AAGAGAGGGA
    GAAAAGAAAG AAGAAGAAAA AGAGGGGGAA GATAAATCAC AACCTAAATC AATCAGAGAA
    CGAAGGCGAC CAAGAGAGAA AAGAAGATCT ACGGGAGTTT CGTTTTGGAC ACAAGATAGT
    GATGAAAATG AACAAGAACA ACAATCTGAT ACAGAGGAGG GATCCAATAA GAAAGAAGTT
    CAGACGGATT CCATTTCTAG ATATGAAATC AGTTCTACAT CAGCTAGTGA TCGATACGAT
    TCCTTGCTGG GTCGCTCTGG ATCATACAGT TACTCAGAAG AAAGAAAACC TTACAGTAGC
    AGGCTAGAAA AGGATGATTC AACTGACTTT AAAAAGCTTT ATGAACAAAT TCTAGCTGAA
    AATGAAAAGC TGAAAGCGCA GCTCCATGAT ACAAATATGG AACTGACAGA TCTTAAATTA
    CAATTGGAAA AAGCCACCCA GAGACAAGAA AGATTCGCTG ATAGGTCACT ATTAGAAATG
    GAAAAAAGGG AACGAAGAGC TCTAGAAAGA AGAATATCTG AAATGGAAGA AGAGCTGAAA
    ATGTTACCAG ACCTGAAAGC AGACAACCAG AGGCTAAAAG ATGAAAATGG GGCCTTGATC
    AGAGTTATAA GCAAACTTTC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847749.1
    CDS314..3400
    Translation

    Target ORF information:

    RefSeq Version XM_024991981.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991981.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAACA ATAGGGAGGA GTCGTTCCAC CCGAACCAGA
    GAACAAGAAA ATGAAGAAAA AGAGGAAAAA GAGAAACAGG ATAAAGAAAA GCAGGAAGAA
    AAGAAGGAAT CAGAAACATC TAGAGAAGAT GAATATAAAC AAAAGTACTC CAGAACATAT
    GATGAGGCTT ATCAACGCTA CAGACCAGTA TCAACTTCAA GTTCAACCAC TCCCTCCTCT
    TTACTTTCTA CAATGAGCAG TTCACTTTAT GCTTCAAGTC AATTAAACAG GCCAAATAGT
    CTTGTAGGTA TAACTTCTGC TTACTCCAGA GGATTAACTA AGGAAAATGA AAGAGAGGGA
    GAAAAGAAAG AAGAAGAAAA AGAGGGGGAA GATAAATCAC AACCTAAATC AATCAGAGAA
    CGAAGGCGAC CAAGAGAGAA AAGAAGATCT ACGGGAGTTT CGTTTTGGAC ACAAGATAGT
    GATGAAAATG AACAAGAACA ACAATCTGAT ACAGAGGAGG GATCCAATAA GAAAGAAGTT
    CAGACGGATT CCATTTCTAG ATATGAAATC AGTTCTACAT CAGCTAGTGA TCGATACGAT
    TCCTTGCTGG GTCGCTCTGG ATCATACAGT TACTCAGAAG AAAGAAAACC TTACAGTAGC
    AGGCTAGAAA AGGATGATTC AACTGACTTT AAAAAGCTTT ATGAACAAAT TCTAGCTGAA
    AATGAAAAGC TGAAAGCGCA GCTCCATGAT ACAAATATGG AACTGACAGA TCTTAAATTA
    CAATTGGAAA AAGCCACCCA GAGACAAGAA AGATTCGCTG ATAGGTCACT ATTAGAAATG
    GAAAAAAGGG AACGAAGAGC TCTAGAAAGA AGAATATCTG AAATGGAAGA AGAGCTGAAA
    ATGTTACCAG ACCTGAAAGC AGACAACCAG AGGCTAAAAG ATGAAAATGG GGCCTTGATC
    AGAGTTATAA GCAAACTTTC CAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234516
    Clone ID Related Accession (Same CDS sequence) XM_024991982.1
    Accession Version XM_024991982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3051bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTCTA GTGTTCCAAG CACCACATCA
    ACACCAACAG TTACCTCTGC AGCTGGGCTT CAGAAAAGCC TGCTTTCCAG CACAAGCACT
    ACTACAAAGA TTACAACGGG TTCTTCCTCA GCAGGCACAC AAAGCAGTAC CTCAAATCGT
    TTGTGGGCTG AGGATAGTAC TGAAAAGGAA AAGGACAGTG TTCCTACGGC AGTGACCATT
    CCTGTTGCTC CAACTGTTGT TAATGCTGCA GCTTCTACCA CAACCCTGAC TACAACTACT
    GCTGGCACTG TCTCCTCCAC TGCAGAGGTC AGGGAGAGAC GCAGATCATA CCTCACTCCT
    GTTAGAGATG AAGAGTCTGA ATCCCAAAGA AAAGCAAGAT CTAGACAAGC AAGACAGTCT
    AGAAGATCAA CACAGGGGGT AACATTGACT GATCTTCAGG AAGCTGAAAA AACAATAGGG
    AGGAGTCGTT CCACCCGAAC CAGAGAACAA GAAAATGAAG AAAAAGAGGA AAAAGAGAAA
    CAGGATAAAG AAAAGCAGGA AGAAAAGAAG GAATCAGAAA CATCTAGAGA AGATGAATAT
    AAACAAAAGT ACTCCAGAAC ATATGATGAG GCTTATCAAC GCTACAGACC AGTATCAACT
    TCAAGTTCAA CCACTCCCTC CTCTTTACTT TCTACAATGA GCAGTTCACT TTATGCTTCA
    AGTCAATTAA ACAGGCCAAA TAGTCTTGTA GGTATAACTT CTGCTTACTC CAGAGGATTA
    ACTAAGGAAA ATGAAAGAGA GGGAGAAAAG AAAGAAGAAG AAAAAGAGGG GGAAGATAAA
    TCACAACCTA AATCAATCAG AGAACGAAGG CGACCAAGAG AGAAAAGAAG ATCTACGGGA
    GTTTCGTTTT GGACACAAGA TAGTGATGAA AATGAACAAG AACAACAATC TGATACAGAG
    GAGGGATCCA ATAAGAAAGA AGTTCAGACG GATTCCATTT CTAGATATGA AATCAGTTCT
    ACATCAGCTA GTGATCGATA CGATTCCTTG CTGGGTCGCT CTGGATCATA CAGTTACTCA
    GAAGAAAGAA AACCTTACAG TAGCAGGCTA GAAAAGGATG ATTCAACTGA CTTTAAAAAG
    CTTTATGAAC AAATTCTAGC TGAAAATGAA AAGCTGAAAG CGCAGCTCCA TGATACAAAT
    ATGGAACTGA CAGATCTTAA ATTACAATTG GAAAAAGCCA CCCAGAGACA AGAAAGATTC
    GCTGATAGGT CACTATTAGA AATGGAAAAA AGGGAACGAA GAGCTCTAGA AAGAAGAATA
    TCTGAAATGG AAGAAGAGCT GAAAATGTTA CCAGACCTGA AAGCAGACAA CCAGAGGCTA
    AAAGATGAAA ATGGGGCCTT GATCAGAGTT ATAAGCAAAC TTTCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847750.1
    CDS251..3301
    Translation

    Target ORF information:

    RefSeq Version XM_024991982.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTCTA GTGTTCCAAG CACCACATCA
    ACACCAACAG TTACCTCTGC AGCTGGGCTT CAGAAAAGCC TGCTTTCCAG CACAAGCACT
    ACTACAAAGA TTACAACGGG TTCTTCCTCA GCAGGCACAC AAAGCAGTAC CTCAAATCGT
    TTGTGGGCTG AGGATAGTAC TGAAAAGGAA AAGGACAGTG TTCCTACGGC AGTGACCATT
    CCTGTTGCTC CAACTGTTGT TAATGCTGCA GCTTCTACCA CAACCCTGAC TACAACTACT
    GCTGGCACTG TCTCCTCCAC TGCAGAGGTC AGGGAGAGAC GCAGATCATA CCTCACTCCT
    GTTAGAGATG AAGAGTCTGA ATCCCAAAGA AAAGCAAGAT CTAGACAAGC AAGACAGTCT
    AGAAGATCAA CACAGGGGGT AACATTGACT GATCTTCAGG AAGCTGAAAA AACAATAGGG
    AGGAGTCGTT CCACCCGAAC CAGAGAACAA GAAAATGAAG AAAAAGAGGA AAAAGAGAAA
    CAGGATAAAG AAAAGCAGGA AGAAAAGAAG GAATCAGAAA CATCTAGAGA AGATGAATAT
    AAACAAAAGT ACTCCAGAAC ATATGATGAG GCTTATCAAC GCTACAGACC AGTATCAACT
    TCAAGTTCAA CCACTCCCTC CTCTTTACTT TCTACAATGA GCAGTTCACT TTATGCTTCA
    AGTCAATTAA ACAGGCCAAA TAGTCTTGTA GGTATAACTT CTGCTTACTC CAGAGGATTA
    ACTAAGGAAA ATGAAAGAGA GGGAGAAAAG AAAGAAGAAG AAAAAGAGGG GGAAGATAAA
    TCACAACCTA AATCAATCAG AGAACGAAGG CGACCAAGAG AGAAAAGAAG ATCTACGGGA
    GTTTCGTTTT GGACACAAGA TAGTGATGAA AATGAACAAG AACAACAATC TGATACAGAG
    GAGGGATCCA ATAAGAAAGA AGTTCAGACG GATTCCATTT CTAGATATGA AATCAGTTCT
    ACATCAGCTA GTGATCGATA CGATTCCTTG CTGGGTCGCT CTGGATCATA CAGTTACTCA
    GAAGAAAGAA AACCTTACAG TAGCAGGCTA GAAAAGGATG ATTCAACTGA CTTTAAAAAG
    CTTTATGAAC AAATTCTAGC TGAAAATGAA AAGCTGAAAG CGCAGCTCCA TGATACAAAT
    ATGGAACTGA CAGATCTTAA ATTACAATTG GAAAAAGCCA CCCAGAGACA AGAAAGATTC
    GCTGATAGGT CACTATTAGA AATGGAAAAA AGGGAACGAA GAGCTCTAGA AAGAAGAATA
    TCTGAAATGG AAGAAGAGCT GAAAATGTTA CCAGACCTGA AAGCAGACAA CCAGAGGCTA
    AAAGATGAAA ATGGGGCCTT GATCAGAGTT ATAAGCAAAC TTTCCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234517
    Clone ID Related Accession (Same CDS sequence) XM_024991983.1
    Accession Version XM_024991983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase 1 regulatory subunit 12A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAACA ATAGGGAGGA GTCGTTCCAC CCGAACCAGA
    GAACAAGAAA ATGAAGAAAA AGAGGAAAAA GAGAAACAGG ATAAAGAAAA GCAGGAAGAA
    AAGAAGGAAT CAGAAACATC TAGAGAAGAT GAATATAAAC AAAAGTACTC CAGAACATAT
    GATGAGGCTT ATCAACGCTA CAGACCAGTA TCAACTTCAA GTTCAACCAC TCCCTCCTCT
    TTACTTTCTA CAATGAGCAG TTCACTTTAT GCTTCAAGTC AATTAAACAG GCCAAATAGT
    CTTGTAGGTA TAACTTCTGC TTACTCCAGA GGATTAACTA AGGAAAATGA AAGAGAGGGA
    GAAAAGAAAG AAGAAGAAAA AGAGGGGGAA GATAAATCAC AACCTAAATC AATCAGAGAA
    CGAAGGCGAC CAAGAGAGAA AAGAAGATCT ACGGGAGTTT CGTTTTGGAC ACAAGATAGT
    GATGAAAATG AACAAGAACA ACAATCTGAT ACAGAGGAGG GATCCAATAA GAAAGAAGTT
    CAGACGGATT CCATTTCTAG ATATGAAATC AGTTCTACAT CAGCTAGTGA TCGATACGAT
    TCCTTGCTGG GTCGCTCTGG ATCATACAGT TACTCAGAAG AAAGAAAACC TTACAGTAGC
    AGGCTAGAAA AGGATGATTC AACTGACTTT AAAAAGCTTT ATGAACAAAT TCTAGCTGAA
    AATGAAAAGC TGAAAGCGCA GCTCCATGAT ACAAATATGG AACTGACAGA TCTTAAATTA
    CAATTGGAAA AAGCCACCCA GAGACAAGAA AGATTCGCTG ATAGGTCACT ATTAGAAATG
    GAAAAAAGGG TGACCGGCAA GAGTCAGTAT CTTCTCGGCG GAACGAAGAG CTCTAGAAAG
    AAGAATATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847751.1
    CDS251..3262
    Translation

    Target ORF information:

    RefSeq Version XM_024991983.1
    Organism Bos taurus(cattle)
    Definition Bos taurus protein phosphatase 1 regulatory subunit 12A (PPP1R12A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAAGATGG CGGACGCGAA GCAGAAGCGG AACGAGCAGC TGAAGCGCTG GATCGGCTCC 
    GAGACGGACC TCGAGCCTCC TGTGGTGAAG CGCCAGAAGA CCAAGGTGAA GTTCGATGAT
    GGCGCCGTCT TCCTGGCTGC TTGCTCCAGC GGCGACACGG ACGAGGTTCT CAAGCTGCTG
    CACCGCGGCG CCGACATCAA TTACGCCAAT GTGGACGGAC TCACTGCCCT GCACCAGGCT
    TGCATTGATG ACAATGTTGA TATGGTGAAG TTTCTGGTAG AAAATGGAGC AAATATTAAT
    CAACCTGATA ATGAAGGCTG GATACCTCTG CATGCAGCTG CTTCCTGTGG ATATCTTGAT
    ATTGCAGAGT TTTTGATTGG TCAGGGAGCA CATGTAGGAG CTGTCAACAG TGAAGGAGAC
    ACACCTTTAG ATATCGCTGA GGAGGAGGCA ATGGAAGAGC TACTGCAGAA TGAAGTTAAT
    CGGCAAGGGG TTGATATAGA AGCAGCTCGA AAAGAAGAGG AACGAATAAT GCTTAGAGAT
    GCCAGACAGT GGTTAAATAG TGGTCATATA AATGATGTCC GGCATGCAAA ATCTGGAGGT
    ACAGCGCTTC ACGTTGCAGC CGCCAAGGGC TATACAGAAG TTTTAAAACT TTTAATACAG
    GCAGGCTATG ATGTTAATAT TAAAGATTAT GATGGCTGGA CACCTCTTCA TGCTGCAGCT
    CATTGGGGTA AAGAAGAAGC ATGTCGGATT TTAGTGGACA ATCTGTGTGA TATGGAGATG
    GTCAACAAAG TGGGCCAGAC AGCCTTTGAT GTAGCAGATG AGGACATTTT AGGATATTTA
    GAAGAGTTGC AAAAGAAGCA AAATCTGCTC CATAGTGAAA AACGGGAAAA GAAATCTCCA
    CTAATTGAAT CAACAGCTAA TATGGATAAT AATCAGTCAC AGAAGACCTT TAAAAATAAA
    GAGACATTGA TTATTGAGCC TGAGAAAAAT GCATCCCGTA TTGAATCTCT GGAACAAGAA
    AAGGTTGATG ATGAAGAAGA AGGAAAGAAA GATGAATCTA GTTGCTCTAG TGAAGAAGAT
    GAGGAAGATG ACTCAGAATC AGAAGCTGAA ACAGATAAGA CAAAACCCAT GGCTTCTGTA
    ACTAATGCCA ATACTTCTAG TACACAAGCA GCTCCTGTAG CTGTTACAAC ACCTACTGTG
    TCATCTGGTC AGCCAACACC TACATCACCT ATTAAAAAGT TTCCAGCTTC AGCTACAAAA
    ATTTCTCCCA AAGAAGAAGA GAGAAAAGAT GAGTCCCCTG CATCTTGGAG GTTAGGACTT
    AGAAAGACGG GCAGTTACGG TGCACTTGCA GAAATCACAG CATCTAAAGA AGCTCAGAAG
    GAAAAAGACA CTGCAGGTGT CATGCGCTCA GCTTCCAGTC CCAGACTTTC CTCCTCTTTG
    GATAATAAAG AAAAGGAGAA AGATAGTAAA GGAACTAAGC TTGCATATGT TACGCCTACA
    ATACCAAGGC GGCTTGCCAG TACATCTGAC ATTGAAGAGA AAGAAAACAG AGATTCTTCA
    AGTTTGCGAA CAAGTAGTTC ATACACAAGA AGAAAATGGG AAGATGATCT TAAAAAGAAT
    AGCTCAATTA ATGAAGGATC AACTTATCAC AAAAGTTGCT CCTTTGGTAG AAGACAAGAT
    GATTTGATTA GTTCTAGTGT TCCAAGCACC ACATCAACAC CAACAGTTAC CTCTGCAGCT
    GGGCTTCAGA AAAGCCTGCT TTCCAGCACA AGCACTACTA CAAAGATTAC AACGGGTTCT
    TCCTCAGCAG GCACACAAAG CAGTACCTCA AATCGTTTGT GGGCTGAGGA TAGTACTGAA
    AAGGAAAAGG ACAGTGTTCC TACGGCAGTG ACCATTCCTG TTGCTCCAAC TGTTGTTAAT
    GCTGCAGCTT CTACCACAAC CCTGACTACA ACTACTGCTG GCACTGTCTC CTCCACTGCA
    GAGGTCAGGG AGAGACGCAG ATCATACCTC ACTCCTGTTA GAGATGAAGA GTCTGAATCC
    CAAAGAAAAG CAAGATCTAG ACAAGCAAGA CAGTCTAGAA GATCAACACA GGGGGTAACA
    TTGACTGATC TTCAGGAAGC TGAAAAAACA ATAGGGAGGA GTCGTTCCAC CCGAACCAGA
    GAACAAGAAA ATGAAGAAAA AGAGGAAAAA GAGAAACAGG ATAAAGAAAA GCAGGAAGAA
    AAGAAGGAAT CAGAAACATC TAGAGAAGAT GAATATAAAC AAAAGTACTC CAGAACATAT
    GATGAGGCTT ATCAACGCTA CAGACCAGTA TCAACTTCAA GTTCAACCAC TCCCTCCTCT
    TTACTTTCTA CAATGAGCAG TTCACTTTAT GCTTCAAGTC AATTAAACAG GCCAAATAGT
    CTTGTAGGTA TAACTTCTGC TTACTCCAGA GGATTAACTA AGGAAAATGA AAGAGAGGGA
    GAAAAGAAAG AAGAAGAAAA AGAGGGGGAA GATAAATCAC AACCTAAATC AATCAGAGAA
    CGAAGGCGAC CAAGAGAGAA AAGAAGATCT ACGGGAGTTT CGTTTTGGAC ACAAGATAGT
    GATGAAAATG AACAAGAACA ACAATCTGAT ACAGAGGAGG GATCCAATAA GAAAGAAGTT
    CAGACGGATT CCATTTCTAG ATATGAAATC AGTTCTACAT CAGCTAGTGA TCGATACGAT
    TCCTTGCTGG GTCGCTCTGG ATCATACAGT TACTCAGAAG AAAGAAAACC TTACAGTAGC
    AGGCTAGAAA AGGATGATTC AACTGACTTT AAAAAGCTTT ATGAACAAAT TCTAGCTGAA
    AATGAAAAGC TGAAAGCGCA GCTCCATGAT ACAAATATGG AACTGACAGA TCTTAAATTA
    CAATTGGAAA AAGCCACCCA GAGACAAGAA AGATTCGCTG ATAGGTCACT ATTAGAAATG
    GAAAAAAGGG TGACCGGCAA GAGTCAGTAT CTTCTCGGCG GAACGAAGAG CTCTAGAAAG
    AAGAATATCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.