DGKE cDNA ORF clone, Bos taurus(cattle)

The following DGKE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa01668 NM_001192930.3
Latest version!
Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa29372 NM_001192930.2
Latest version!
Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa01668 XM_005219865.4
Latest version!
Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa29372 XM_005219865.3
Latest version!
Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa53833 XM_010815687.2
Latest version!
Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa226465 XM_010815687.3
Latest version!
Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa01668 NM_001192930.1 Bos taurus diacylglycerol kinase, epsilon 64kDa (DGKE), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa01668
    Clone ID Related Accession (Same CDS sequence) NM_001192930.1 , XM_005219865.4 , NM_001192930.3
    Accession Version NM_001192930.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-23
    Organism Bos taurus(Cattle)
    Product diacylglycerol kinase epsilon
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03078107.1, AAFC03078167.1 and AAFC03124418.1. On Jul 17, 2010 this sequence version replaced XM_618342.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179859.1
    CDS1..1695
    Translation

    Target ORF information:

    RefSeq Version NM_001192930.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus diacylglycerol kinase, epsilon 64kDa (DGKE), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192930.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01668
    Clone ID Related Accession (Same CDS sequence) NM_001192930.1 , XM_005219865.4 , NM_001192930.3
    Accession Version NM_001192930.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase epsilon
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1. On May 29, 2018 this sequence version replaced NM_001192930.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571254.30610.1, SRR5571254.16311.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179859.3
    CDS1..1695
    Translation

    Target ORF information:

    RefSeq Version NM_001192930.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192930.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29372
    Clone ID Related Accession (Same CDS sequence) XM_005219865.3 , NM_001192930.2
    Accession Version NM_001192930.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase epsilon
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02048261.1. On Jun 30, 2017 this sequence version replaced NM_001192930.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403653, SAMN02822088 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179859.2
    CDS1..1695
    Translation

    Target ORF information:

    RefSeq Version NM_001192930.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase epsilon (DGKE), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192930.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01668
    Clone ID Related Accession (Same CDS sequence) NM_001192930.1 , XM_005219865.4 , NM_001192930.3
    Accession Version XM_005219865.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase epsilon isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219922.2
    CDS228..1922
    Translation

    Target ORF information:

    RefSeq Version XM_005219865.4
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005219865.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29372
    Clone ID Related Accession (Same CDS sequence) XM_005219865.3 , NM_001192930.2
    Accession Version XM_005219865.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase epsilon isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104487.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005219922.1
    CDS225..1919
    Translation

    Target ORF information:

    RefSeq Version XM_005219865.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005219865.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGCTAGC CTCTAAACTT GGAAAGCAGT GGACTCCATT AATAATCCTG
    GCCAACTCTC GTAGTGGGAC TAACATGGGA GAAGGACTGC TGGGAGAGTT CAGGATCCTC
    CTAAATCCCG TCCAGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa53833
    Clone ID Related Accession (Same CDS sequence) XM_010815687.2
    Accession Version XM_010815687.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase epsilon isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104487.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813989.1
    CDS226..1800
    Translation

    Target ORF information:

    RefSeq Version XM_010815687.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815687.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GAATAAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226465
    Clone ID Related Accession (Same CDS sequence) XM_010815687.3
    Accession Version XM_010815687.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase epsilon isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010813989.2
    CDS229..1803
    Translation

    Target ORF information:

    RefSeq Version XM_010815687.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase epsilon (DGKE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815687.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGGAAGGCC AGGAGCGGCC GGCGCCCCCC GCCAGCCTGT TTGCGGACGG GCACCTGGTC 
    CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGTTCTTCA CCTTCTGGTG CAGCGTCCAG
    CGGTCGCGCC GGCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGCTGGCGC
    GACACGGATC TGTTCAGCCA GCCCACCTAC TGCTGTGTGT GCGCGCAGCA CATCCTGCAG
    GGCGCCTTCT GCGACTGCTG TGGGCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC
    AAGCGCTTCC CCTGCAAGGA GATCATGCTC AAGAGTGACA GCAAGGCCGT GGACGCCATG
    CCCCACCACT GGATCCGGGG AAACGTCCCC CTGTGCAGTT ACTGTGTGGT TTGCAAGCAA
    CAGTGCGGCA ACCAGCCCAA GCTCTGCGAT TACAGGTGCG TTTGGTGTCA AAAAACAGTA
    CATGATGAGT GTATGAAAAA TAGCTTAAGG AATGAAAAGT GTGATTTTGG AGAATTCAAA
    AACCTCATCA TTCCACCAAG TTATTTAACC TCCATTAATC ATATGCGTAA AGACAAAAAA
    ACAGATTATG AAATGGTTTT TGATGTTACT AAAACTCCCC CCACCAAAGC CCTACAACTT
    TGTACTCTTC TTCCCTATGA CTCAGCTCGA GTGCTTGTCT GTGGAGGGGA TGGGACTGTC
    GGCTGGGTCC TGGATGCCCT TGACGAAATG AAAATTAAGG GACAAGAGAA ATACGTTCCA
    CGAGTTGCGG TCTTGCCTCT GGGAACAGGC AATGATCTCT CCAACACCTT GGGGTGGGGT
    ACAGGTTATG CGGGAGAAAT CCCAGTTGCA CAAGTCTTAA GAAACGTGAT GGAGGCAGAT
    GGAGTTAAAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA CAACTTAAGA
    AAACCCAAGG AATTCACAAT GAACAACTAT TTTTCTATTG GACCTGATGC TCTCATGGCC
    CTCAATTTTC ACGCTCATCG TGAGAAGGCC CCATCTCTGT TTTCTAGCAG AATTCTTAAT
    AAGGCTGTTT ACTTATTTTA TGGAACCAAA GATTGTTTAG TGCAAGAATG TAAAGATTTG
    AATAAAAAAG TTGAGCTAGA GCTGGATGGT GAGCGAGTAG AACTGCCGAA TTTGGAAGGC
    ATTATCGTTC TGAATATCGG CTACTGGGGT GGTGGCTGCA GACTGTGGGA AGGGATGGGA
    GACGAGACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAT TGGAAGTCGT CGGAGTATAT
    GGGTCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCGA ATCCTTTTCG AATAGGACAA
    GCACATACAG TGCGACTGAT CTTGAAGTGC TCGATGATGC CAATGCAGGT AGATGGGGAG
    CCGTGGGCCC AGGGGCCCTG CACTGTCACC ATAACCCACA AGACACACGC ACTGATGTTG
    TATTTCTCTG GAGAACAAAC GGATGACGAC GTCTCTAGTA CTTCAGATCA AGAGGATACA
    AAGGAAACGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.