GRIN2B cDNA ORF clone, Bos taurus(cattle)

The following GRIN2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRIN2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239137 NM_001192921.3
Latest version!
Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa19686 NM_001192921.2
Latest version!
Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa36350 NM_001192921.1
Latest version!
Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa19686 XM_005206988.3
Latest version!
Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239137
    Clone ID Related Accession (Same CDS sequence) NM_001192921.3
    Accession Version NM_001192921.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product glutamate receptor ionotropic, NMDA 2B precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On May 30, 2018 this sequence version replaced NM_001192921.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145419, SAMN03145422 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ACGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179850.1
    CDS1..4494
    Translation

    Target ORF information:

    RefSeq Version NM_001192921.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192921.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ACGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19686
    Clone ID Related Accession (Same CDS sequence) NM_001192921.2 , XM_005206988.3
    Accession Version NM_001192921.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product glutamate receptor ionotropic, NMDA 2B precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02014253.1, DAAA02014255.1, DAAA02014257.1, DAAA02014262.1 and DAAA02014263.1. On Jun 30, 2017 this sequence version replaced NM_001192921.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN04019643 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179850.1
    CDS1..4494
    Translation

    Target ORF information:

    RefSeq Version NM_001192921.2
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192921.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36350
    Clone ID Related Accession (Same CDS sequence) NM_001192921.1
    Accession Version NM_001192921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product glutamate receptor ionotropic, NMDA 2B precursor
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03086648.1, AAFC03071459.1, AAFC02060016.1 and AAFC03054766.1. On Jul 17, 2010 this sequence version replaced XM_617989.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179850.1
    CDS1..4494
    Translation

    Target ORF information:

    RefSeq Version NM_001192921.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CTGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19686
    Clone ID Related Accession (Same CDS sequence) NM_001192921.2 , XM_005206988.3
    Accession Version XM_005206988.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product glutamate receptor ionotropic, NMDA 2B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103935.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207045.1
    CDS780..5273
    Translation

    Target ORF information:

    RefSeq Version XM_005206988.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206988.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGCCCA GAGCGGAGTG CTGTTCCCCC AAGTTCTGGT TGGTGCTGGC TGTCCTGGCC 
    GTGTCCGGCA GCAGAGCTCG TTCTCAGAAG AGCCCGCCCA GCATCGGCAT TGCTGTCATC
    CTCGTGGGCA CTTCAGACGA GGTGGCCATC AAGGACGCCC ACGAGAAAGA TGATTTCCAC
    CATCTCTCCG TGGTGCCCCG CGTGGAGCTG GTGGCCATGA ACGAGACGGA CCCGAAGAGC
    ATCATAACCC GCATCTGTGA TCTCATGTCC GACCGGAAGA TCCAGGGGGT GGTGTTTGCT
    GATGACACAG ACCAGGAAGC CATTGCCCAG ATCCTCGACT TCATTTCAGC CCAGACTCTC
    ACGCCCATCC TGGGCATCCA CGGGGGCTCC TCGATGATAA TGGCGGACAA GGATGAATCC
    TCCATGTTCT TCCAGTTCGG CCCATCAATT GAACAGCAGG CTTCCGTGAT GCTCAACATC
    ATGGAGGAGT ATGACTGGTA CATCTTCTCT ATCGTCACCA CCTACTTCCC TGGCTACCAG
    GACTTTGTCA ACAAGATTCG CAGCACCATT GAGAACAGCT TCGTGGGCTG GGAGCTGGAG
    GAGGTCCTTC TGCTGGACAT GTCCTTGGAC GATGGGGATT CTAAGATCCA GAACCAGCTC
    AAGAAACTTC AGAGCCCCAT CATTCTTCTT TACTGTACTA AGGAAGAGGC CACCTACATC
    TTCGAAGTGG CTAACTCAGT AGGGCTGACC GGCTACGGCT ACACGTGGAT TGTGCCTAGT
    CTGGTGGCGG GGGACACGGA CACAGTGCCC TCGGAGTTCC CCACCGGCCT GGTCTCTGTG
    TCCTACGACG AGTGGGACTA CGGCCTCCCT GCCAGAGTGA GGGACGGGAT CGCCATCATC
    ACCACCGCGG CCTCCGACAT GCTGTCCGAG CACAGCTTCA TCCCCGAGCC CAAGAGCAGC
    TGCTACAACA CCCACGAGAA GAGGATCTAC CAGTCCAATA TGCTGAACAG GTATCTGATC
    AATGTGACTT TTGAAGGGAG AAATCTGTCC TTCAGTGAAG ACGGCTACCA GATGCACCCA
    AAGCTGGTGA TCATCCTTCT GAACAAGGAG AGGAAGTGGG AGAGGGTAGG GAAGTGGAAA
    GACAAGTCCC TGCAGATGAA GTACTACGTG TGGCCCCGAA TGTGTCCTGA GACGGAAGAG
    CAGGAGGATG ACCACCTGAG CATCGTAACG TTGGAGGAAG CCCCATTTGT CATTGTGGAG
    AGCGTGGACC CTCTGAGTGG AACCTGCATG AGGAACACGG TCCCCTGCCA AAAGCGCATC
    ATCTCCGAAA ATAAAACAGA TGAGGAGCCA GGTTACATCA AAAAGTGCTG CAAGGGGTTC
    TGTATTGATA TCCTTAAGAA AATTTCTAAA TCTGTGAAGT TCACCTATGA TCTTTACCTG
    GTTACCAATG GCAAGCATGG GAAGAAAATC AATGGAACCT GGAATGGTAT GATTGGAGAG
    GTGGTCATGA AGAGGGCCTA CATGGCAGTG GGGTCGCTGA CCATCAACGA GGAGCGATCC
    GAGGTGGTTG ATTTCTCCGT GCCCTTCATA GAGACTGGCA TCAGCGTCAT GGTGTCACGC
    AGCAATGGGA CTGTCTCACC TTCTGCCTTC TTAGAGCCAT TCAGCGCTGA CGTGTGGGTC
    ATGATGTTTG TGATGCTCCT CATCGTCTCA GCCGTGGCTG TCTTTGTCTT CGAGTACTTT
    AGCCCCGTGG GTTACAACAG GTGCCTCGCT GACGGCAGAG AGCCAGGTGG CCCATCTTTC
    ACCATCGGCA AAGCTATTTG GTTACTGTGG GGTCTGGTGT TTAACAACTC CGTCCCCGTG
    CAGAACCCCA AGGGTACCAC GTCCAAGATC ATGGTGTCCG TGTGGGCCTT CTTTGCCGTC
    ATCTTCCTGG CCAGCTACAC TGCCAACTTA GCTGCCTTCA TGATCCAAGA GGAGTATGTG
    GACCAGGTTT CCGGCCTGAG TGACAAAAAG TTCCAGAGAC CTAATGACTT CTCACCCCCT
    TTCCGCTTTG GCACTGTGCC CAACGGCAGC ACAGAGAGGA ATATTCGCAA TAACTATGCA
    GAAATGCATG CCTACATGGG GAAGTTCAAC CAGAGAGGTG TCGATGATGC ACTGCTCTCC
    CTGAAAACAG GGAAACTGGA TGCCTTCATC TACGATGCAG CCGTGCTGAA CTACATGGCC
    GGTAGAGATG AGGGCTGCAA GCTGGTGACC ATTGGCAGTG GGAAGGTCTT TGCCTCCACT
    GGTTATGGCA TTGCCATCCA AAAAGACTCT GGATGGAAGC GCCAGGTGGA CCTTGCTATC
    CTGCAGCTCT TCGGAGATGG GGAGATGGAG GAGCTGGAAG CTCTGTGGCT CACAGGCATC
    TGCCACAATG AGAAGAACGA GGTCATGAGT AGCCAGCTGG ACATCGACAA CATGGCGGGT
    GTCTTCTACA TGCTGGGGGC AGCCATGGCT CTCAGCCTCA TCACCTTCAT CTGTGAACAC
    CTTTTCTATT GGCAGTTCCG GCACTGCTTT ATGGGTGTCT GTTCTGGCAA GCCTGGCATG
    GTCTTCTCCA TCAGCAGAGG TATCTACAGC TGCATCCATG GCGTGGCCAT TGAGGAGCGC
    CAGTCCGTGA TGAACTCCCC CACCGCCACC ATGAACAACA CGCACTCCAA TATCCTGCGT
    CTGCTGCGCA CGGCCAAGAA CATGGCCAAC CTGTCGGGTG TGAATGGCTC ACCGCAGAGC
    GCCCTGGACT TCATCCGCCG CGAGTCGTCT GTCTATGACA TCTCCGAGCA CCGCCGCAGC
    TTCACGCACT CCGACTGCAA GTCCTACAAC AACCCGCCCT GCGAGGAGAA CCTGTTCAGT
    GACTACATCA GTGAGGTGGA AAGGACTTTC GGGAACCTGC AGCTCAAGGA CAGCAACGTG
    TACCAGGACC ACTACCACCA CCACCACCGG CCCCACAGCA TCGGCAGCGC CAGCTCCATA
    GATGGGCTCT ACGACTGTGA CAACCCGCCC TTCACCACGC AGCCCCGGGC CCTGGGCAAG
    AAGCCCCTGG ACATTGGCCT CCCCTCGTCC AAGCACAGCC AGCTCGGCGA CCTGTACGGC
    AAGTTCTCTT TCAAGAGTGA CCGCTATGGC GGCCACGACG ACCTGATCCG CTCCGACGTG
    TCGGACATCT CCACCCACAC GGTCACCTAC GGCAACATCG AGGGCAATGC GGCCAAGAGA
    CGGAAGCAGC AGTACAAGGA CAGCCTGAAG AAGCGTCCAG CCTCGGCCAA GTCCCGCCGC
    GAGTTCGACG AGATAGAGCT GGCCTACCGC CGCCGGCCGC CCCGCTCCCC AGACCACAAG
    CGCTACTTTC GGGACAAGGA CGGGCTACGG GACTTCTACC TGGACCAGTT CCGTGCCAAG
    GAGACCTCGC CGCACTGGGA GCACGTGGAC CTGACTGACC TCTATAAGGA GCGCGGCGAG
    GATTTCAAGC GCGACTCGGT GGGTGGCGGA GGGCCCTGTA CCAACCGAGC TCATCACCTT
    AAGCACGGCA CGGGCGACAA GCACGGCGGG GGGGTCGTCG GTGGGGTCCC GGCCCCCTGG
    GAGAAGAACC TGACCAACGT GGACTGGGAG GAGCGCCCTG GGGGCAACTT CTGCCGCAGC
    TGCCCATCCA AGCTGCACAA CTACTCGACG ACGACGGCAG CAACGGCAGT GGTGGCTGGC
    CAGAACTCAG GCAGGCCGGC CTGCATCCGC TGCGAGGCGT GCAAGAAGGC CGGCAACCTG
    TATGACATCA GCGAGGACAA CTCGCTGCAG GAGTTGGATC AGCCGGCCGC CCCGGTGGCG
    GCCGTGACAT CCGCCGCGTC TAAGTACCCG CAGAGCCCCA CCAACTCCAA GGCGCAGAAG
    AAGCCCCGGA ACAAGCTGCG GCGCCAACAC TCCTACGACA CGTTCGTGGA CCTGCAGAAA
    GAAGAGGCTG CCCTGGCCCC GCGCAGCGTG AGCCTCAAGG ACAAGGGCCG CTTCCTGGAC
    GGCAGCCCCT ATGCGCACAT GTTCGAGATG CCGGCGGGCG AGAGCGCTTT CAGTGGCAGC
    AACGACGGCA AGGCGGCGGC GCCGGCCGCA GGACATCACC ACCATGGCAA TCCCGGCGGC
    GGCGGTGGCT ACATGCTCAG CAAGTCACTC TACCCCGACC GGGTCACGCA GAACCCTTTC
    ATCCCCACTT TTGGGGACGA CCAGTGCTTG CTCCACGGCA GCAAATCCTA CTTCTTCAGG
    CAGCCCACGG TGGCTGGTGC ACCGAAGGCC AGGCCGGACT TCCGGGCCCT TGTGAGCAAC
    AAGCCGGTGG TCTCGGCCCT GCACGGGGCT GTGCCGGGCC GCTTCCAGAA AGACATCTGT
    ATAGGGAACC AGTCCAACCC CTGTGTGCCT AACAACAAAA ACCCCAGGGC TTTCAATGGC
    TCCAGCAATG GGCATGTGTA TGAGAAACTG TCTAGTATTG AGTCTGATGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.